ライフサイエンスコーパス: Methanosarcina

1 le for genetic studies in the archaeal genus Methanosarcina.

2 stic methanogenesis in both Methanosaeta and Methanosarcina.

3 ed a markerless tRNA(Pyl) deletion strain of Methanosarcina acetivorans (DeltapylT) that cannot decod

4 t comparison of histones from M. jannaschii, Methanosarcina acetivorans (largest Archaeal genome, 5.8

5 ere identified in M. jannaschii (Mj0601) and Methanosarcina acetivorans (Ma2851), and recombinant Ma2

6 TLPs from Bacillus thuringiensis (BtTLP) and Methanosarcina acetivorans (MaTLP) display biochemical p

7 naturally "truncated" homologs of NifB from Methanosarcina acetivorans (NifB(Ma)) and Methanobacteri

8 old/one zinc finger replication protein A in Methanosarcina acetivorans and Methanopyrus kandleri exh

9 ence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei.

10 nesis, methyl-coenzyme M reductase (MCR), in Methanosarcina acetivorans and tested whether its cellul

11 e isozymes of methanol methyltransferases in Methanosarcina acetivorans C2A and are among the most hi

12 The mtaA1, mtaA2, and mtbA genes of Methanosarcina acetivorans C2A encode putative methanol-

13 Methanosarcina acetivorans C2A encodes three putative hy

14 Methanosarcina acetivorans C2A is able to convert severa

15 clones were isolated after transformation of Methanosarcina acetivorans C2A with the mutagenized plas

16 en in this process, we tested the ability of Methanosarcina acetivorans C2A, a metabolically versatil

17 oson mutagenesis of a methanogenic archaeon, Methanosarcina acetivorans C2A, which because of its ind

18 The corresponding mutation in Methanosarcina acetivorans C2A, which cannot grow on H(2

19 sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A.

20 ere the first use of directed mutagenesis in Methanosarcina acetivorans C2A.

21 s and characterized the encoded enzymes from Methanosarcina acetivorans C2A.

22 a methanogen into an acetogen and show that Methanosarcina acetivorans can dispense with methanogene

23 lyses to study the structure/function of the Methanosarcina acetivorans clamp loader or replication f

24 rolysine, a 761 base-pair genomic segment in Methanosarcina acetivorans containing the pylT gene (enc

25 The genome of Methanosarcina acetivorans encodes three homologs, initi

26 Unlike most organisms, Methanosarcina acetivorans harbors multiple functional R

27 Interestingly, the euryarchaeon Methanosarcina acetivorans harbors multiple functional R

28 The Methanosarcina acetivorans hdrED1 operon is constitutive

29 on of the slow-growing methanogenic archaeon Methanosarcina acetivorans Introduction of both insertio

30 multiheme c-type cytochrome called MmcA from Methanosarcina acetivorans is important for intracellula

31 ation of McrA from the methanogenic archaeon Methanosarcina acetivorans lacking tfuA and/or ycaO reve

32 se 1 operons (mtaCB1, mtaCB2, and mtaCB3) in Methanosarcina acetivorans led to the suggestion that ea

33 onsible for formation of S-methylcysteine in Methanosarcina acetivorans McrA.

34 n methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically labeled with 14

35 The regulation of the Methanosarcina acetivorans mtsD, mtsF and mtsH genes, wh

36 onance analyses to establish histidine-43 of Methanosarcina acetivorans NifB (MaNifB) as the nitrogen

37 containing NifB protein upon coexpression of Methanosarcina acetivorans nifB, nifS, and nifU genes al

38 standing of this family, a flavoredoxin from Methanosarcina acetivorans of the Archaea domain was pro

39 As the Methanosarcina acetivorans PFOR is comprised of multiple

40 Here we show that Methanosarcina acetivorans possesses three operons encod

41 Methanosarcina acetivorans produces acetate, formate, an

42 , 3046 unique peptides covering 566 distinct Methanosarcina acetivorans proteins were identified from

43 tandem polypeptide repeats that comprise the Methanosarcina acetivorans S-layer protein and propose a

44 haea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical behaviour.

45 Methanosarcina acetivorans strain C2A is a marine methan

46 Methanosarcina acetivorans strains bearing deletions of

47 ein the purification and characterization of Methanosarcina acetivorans subunit D in complex with sub

48 MacDinB-1, the homolog from the euryarchaeon Methanosarcina acetivorans that is characterized in this

49 The Methanosarcina acetivorans Thg1 (MaThg1) gene contains a

50 otein overproduction system was developed in Methanosarcina acetivorans to facilitate biochemical cha

51 ein 10 gene in the methane-producing archaea Methanosarcina acetivorans Using an array of approaches,

52 Here, we study the methanogenic archaeon Methanosarcina acetivorans using assays of ATP hydrolysi

53 ltisubunit sodium/proton antiporter (Mrp) of Methanosarcina acetivorans was investigated with a mutan

54 rotein MA4561 from the methanogenic archaeon Methanosarcina acetivorans was originally predicted to b

55 ologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using ge

56 opy (cryo-EM) structure of the ribosome from Methanosarcina acetivorans, a previously unreported high

57 Methanosarcina acetivorans, a strictly anaerobic methane

58 ization of an unusual flavodoxin (FldA) from Methanosarcina acetivorans, an acetate-utilizing methane

59 iogenesis in the model methanogenic archaeon Methanosarcina acetivorans, and have also identified sub

60 nococcus jannaschii, Archaeoglobus fulgidus, Methanosarcina acetivorans, and Methanosarcina barkeri p

61 chaeoglobus fulgidus, Methanopyrus kandleri, Methanosarcina acetivorans, and Methanosarcina mazei.

62 hlorobaculum tepidum, Magnetococcus marinus, Methanosarcina acetivorans, as well as revisiting the si

63 bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene dele

64 SH) on >14 500 archaeal and bacterial cells (Methanosarcina acetivorans, Sulfolobus acidocaldarius an

65 Gene-deletion and growth experiments in Methanosarcina acetivorans, using sulfide as the sole su

66 In the model methanogen, Methanosarcina acetivorans, we demonstrate that the UAG

67 a representative of this new group of RPA in Methanosarcina acetivorans, we made two deletion mutants

68 termed Hsp60-4 and Hsp60-5, in the archaeon Methanosarcina acetivorans, which also has Hsp60-1, Hsp6

69 Here, the role of SufBC in Methanosarcina acetivorans, which contains two sufCB gen

70 methyltransferase MtmB1, was introduced into Methanosarcina acetivorans.

71 a new form of clamp loader from the archaeon Methanosarcina acetivorans.

72 MaPgb from the strictly anaerobic methanogen Methanosarcina acetivorans.

73 ogs, RPA1, RPA2, and RPA3, from the archaeon Methanosarcina acetivorans.

74 on the naturally occurring plasmid pC2A from Methanosarcina acetivorans.

75 genic growth on methylamines in the archaeon Methanosarcina acetivorans.

76 rized a member of the YrbG family, MaX1 from Methanosarcina acetivorans.

77 mp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans.

78 e family from the methane-producing archaeon Methanosarcina acetivorans.

79 d in Escherichia coli expressing pylBCD from Methanosarcina acetivorans.

80 Although the aceticlastic methanoarchaea Methanosarcina and Methanosaeta employ different enzymes

81 me, genetic analysis in Archaea of the genus Methanosarcina are presented.

82 RNA pyrosequencing revealed the dominance of Methanosarcina at 55-60 degrees C.

83 h electron uptake mechanism of a methanogen, Methanosarcina barkeri (M. barkeri), by coculturing it w

84 to the cultures of a methanogenic archaeon, Methanosarcina barkeri (M. barkeri).

85 Methanosarcina barkeri 227 possesses two clusters of gen

86 nitrogenase structural genes (nifHDK2) from Methanosarcina barkeri 227 was completed in this study b

87 tial reactions of acetyl-CoA cleavage by the Methanosarcina barkeri acetyl-CoA decarbonylase synthase

88 io desulfuricans, Desulfovibrio vulgaris and Methanosarcina barkeri AhbA/B have been produced and the

89 meric iron-sulfur protein, was isolated from Methanosarcina barkeri and is required for in vitro ATP-

90 s of these putative hydrogenases to those of Methanosarcina barkeri and Methanosarcina mazei shows th

91 Species close to Methanosarcina barkeri and Methanothermobacter thermauto

92 extend earlier findings that the CODHs from Methanosarcina barkeri and Oligotropha carboxydovorans e

93 We show here that wild type PylS from Methanosarcina barkeri and PylSn from Desulfitobacterium

94 iting domain homologues (AlaX proteins) from Methanosarcina barkeri and Sulfolobus solfataricus hydro

95 most similar to those of the archaebacterium Methanosarcina barkeri and the delta-purple bacterium De

96 volution reaction (HER) electrocatalysts and Methanosarcina barkeri as a biocatalyst for CO2 fixation

97 an F(420) -reducing [NiFe]-hydrogenase from Methanosarcina barkeri as a model enzyme, we show that t

98 ine methyltransferase of the archaebacterium Methanosarcina barkeri contains a novel amino acid, pyrr

99 ethylamine methyltransferase of the archaeon Methanosarcina barkeri contains a rare amino acid, pyrro

100 coli strain NK3 led to the isolation of the Methanosarcina barkeri cysK gene [encoding O-acetylserin

101 However, Methanosarcina barkeri Delta fpo mutants constructed in

102 lamine methyltransferase, were detectable in Methanosarcina barkeri during early log growth on trimet

103 Activity staining of extracts of Methanosarcina barkeri electrophoresed in polyacrylamide

104 During growth on acetate, Methanosarcina barkeri expresses catabolic enzymes for o

105 In the methanogenic archaeon Methanosarcina barkeri Fusaro, the N5-methyl-tetrahydros

106 e isoleucyl-tRNA synthetase gene (ileS) from Methanosarcina barkeri Fusaro.

107 Methanosarcina barkeri genes cmtA and cmtM encoding both

108 enzyme MT2-A important for methanogenesis in Methanosarcina barkeri growing on methylamines.

109 plays a central role in energy metabolism in Methanosarcina barkeri grown on acetate.

110 llireducens with Geobacter sulfurreducens or Methanosarcina barkeri in which ethanol was the electron

111 These proteins isolated from Methanosarcina barkeri included the previously unidentif

112 onally inserted amino acid, was found in the Methanosarcina barkeri monomethylamine methyltransferase

113 a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase

114 We demonstrate that an orthogonal Methanosarcina barkeri MS pyrrolysyl-tRNA synthetase/tRN

115 bility of hydrogenotrophic methanogenesis in Methanosarcina barkeri MS under simulated Martian surfac

116 e proteins are clustered on 6.8 kb of DNA in Methanosarcina barkeri MS.

117 A hexahistidine-tagged Methanosarcina barkeri mtmB1 gene, encoding monomethylam

118 A series of Methanosarcina barkeri mutants lacking the genes encodin

119 that transcripts of nifHDK2 genes, encoding Methanosarcina barkeri nitrogenase, are present in N2-gr

120 Evidence is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essent

121 cryptofilum PE (CkoPE; 117 amino acids) and Methanosarcina barkeri PE (MbaPE; 151 amino acids)--and

122 us fulgidus, Methanosarcina acetivorans, and Methanosarcina barkeri possess open reading frames with

123 hetase-tRNA(CUA) (MjTyrRS-tRNA(CUA)) and the Methanosarcina barkeri pyrrolysyl-tRNA synthetase-tRNA(C

124 The Methanosarcina barkeri serC gene, encoding phosphoserine

125 lobus acidocaldarius, Haloferax volcanii and Methanosarcina barkeri the secondary structure of RNase

126 Forty-two Methanosarcina barkeri tRNA(Pyl) variants were tested in

127 sozymes from buffer soluble cell extracts of Methanosarcina barkeri was accomplished by use of immobi

128 recently discovered coenzyme M methylase in Methanosarcina barkeri were analyzed.

129 nechocystis PCC6803 and methanogenic archaea Methanosarcina barkeri with the redox cycling of iron, C

130 mparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acet

131 rganisms (e.g., Geobacter sulfurreducens and Methanosarcina barkeri).

132 mbrane-bound NiFe-hydrogenase complexes from Methanosarcina barkeri, Escherichia coli, and Rhodospiri

133 Like the HDR from Methanosarcina barkeri, it was found to contain two disc

134 Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotroph

135 fer RNA (tRNA) synthetases (SerRSs) exist in Methanosarcina barkeri, one of bacterial type and the ot

136 the metabolic networks of Escherichia coli, Methanosarcina barkeri, Staphylococcus aureus, and Sacch

137 ibody detected primarily a 50-kDa protein in Methanosarcina barkeri, which is the mass predicted for

138 omparative transcriptomics on the methanogen Methanosarcina barkeri, which was incubated at 30 C and

139 the chromosome of the methanogenic archaeon Methanosarcina barkeri.

140 vinelandii, Rhodopseudomonas palustris, and Methanosarcina barkeri.

141 on in methylamine methyltransferase genes of Methanosarcina barkeri.

142 These in vitro data suggest that Methanosarcina cells have two pathways for acylating the

143 r hgcA-harboring acetoclastic strains (e.g., Methanosarcina), decreasing methanogen abundance (18-98%

144 We propose that MA3736 be renamed mdrA (methanosarcina disulfide reductase).

145 w that the efficient acetoclastic pathway in Methanosarcina emerged at a time statistically indisting

146 The ileS gene was cloned into a Methanosarcina-Escherichia coli shuttle vector and mutag

147 ented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.

148 in the methanogens Methanothrix sp. MA6 and Methanosarcina flavescens MX5, which switched their meta

149 horizontally transferred to the ancestor of Methanosarcina from a derived cellulolytic organism in t

150 l lysine codon usage is encountered in other Methanosarcina genes.

151 Microbial species assigned to the Methanosarcina genus increased in relative abundance aft

152 ) and ADP-Acs, the extant methanogenic genus Methanosarcina is the only identified Archaeal genus tha

153 ribe a structural dissection of the archaeal Methanosarcina mazei (Mm) GS and its regulation.

154 elated class II photolyase from the archaeon Methanosarcina mazei (MmCPDII) as well as plantal orthol

155 Characterization of SepRS from the mesophile Methanosarcina mazei by gel filtration and nondenaturing

156 We show here that the Methanosarcina mazei casposase can integrate varied form

157 In pulldown experiments with Methanosarcina mazei cell extract, we identify a physiol

158 In contrast, Methanosarcina mazei contains seven AAA proteins, five o

159 ly expressed genes of the freshwater isolate Methanosarcina mazei determined by transcriptional profi

160 the A-type molecular motor A3B3DF, from the Methanosarcina mazei Go1 A-ATP synthase, and the thermop

161 Methanosarcina mazei PylRS (MmPylRS) is arguably one of

162 e determined three crystal structures of the Methanosarcina mazei PylRS complexed with either AMP-PNP

163 e demonstrate that stable integration of the Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)/

164 Using VADER we developed improved mutants of Methanosarcina mazei pyrrolysyl-tRNA, as well as a bacte

165 3', were found in the genome of the archaeon Methanosarcina mazei S-6 that encode the deduced protein

166 nases to those of Methanosarcina barkeri and Methanosarcina mazei shows that each predicted subunit c

167 the ATP:co(I)rrinoid adenosyltransferase in Methanosarcina mazei strain Go1 (open reading frame MM31

168 me (ORF) Mm2058 of the methanogenic archaeon Methanosarcina mazei strain Go1 was shown in vivo and in

169 The cbiZ gene of the methanogenic archaeon Methanosarcina mazei strain Gol was cloned, was overprod

170 Here we show that the archaebacterial Methanosarcina mazei ThrRS efficiently misactivates seri

171 s that exhibit topology simplification), and Methanosarcina mazei topo VI and Sulfolobus shibatae top

172 g ensemble biochemistry, we demonstrate that Methanosarcina mazei topo VI preferentially unlinks, or

173 dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 m

174 propose that MM1854, a homolog of AfpA from Methanosarcina mazei, catalyzes the last step of H4MPT b

175 In the methanogenic archaeon Methanosarcina mazei, it has been shown that the metabol

176 lic activity for three methanogenic archaea: Methanosarcina mazei, M. barkeri and M. soligelidi, was

177 alophilic Archaea (Methanococcus jannaschii, Methanosarcina mazei, Methanobrevibacter smithii) are le

178 with those of Methanosarcina acetivorans and Methanosarcina mazei.

179 us kandleri, Methanosarcina acetivorans, and Methanosarcina mazei.

180 MK enzymes from alternative microbes such as Methanosarcina mazei.

181 25 of Deinococcus radiodurans and MM_0920 of Methanosarcina mazei.

182 s, with Fo and P(i) or with Fo and GDP, from Methanosarcina mazei.

183 l three enzymes in the mesophilic methanogen Methanosarcina mazei.

184 plus), in cells of the methanogenic archaeon Methanosarcina mazei.

185 ragment of chromosomal DNA from the archaeon Methanosarcina mazeii was sequenced and analyzed, and it

186 cus of the mesophilic, methanogenic archaeon Methanosarcina mazeii.

187 , Pyrobaculum, Aeropyrum) and euryarchaeota (Methanosarcina, Methanococcus, Archaeoglobus, Thermoplas

188 cts translation termination, its presence in Methanosarcina mRNA may lead to pyrrolysine (Pyl) incorp

189 at express the Himar1 transposase from known Methanosarcina promoters.

190 Enzymes from species within Methanosarcina, Pseudomonas, Bartonella, Nitrosomonas, T

191 a strong increase of the expression level in Methanosarcina sp. was evidenced after oleate addition.

192 with multicellular life-cycle phases, e.g., Methanosarcina sp., or Anabaena sp., which have more per

193 ry selectable markers that are functional in Methanosarcina species and that express the Himar1 trans

194 de resistance to pseudomonic acid (PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-t

195 Methanosarcina species possess three operons (mtaCB1, mt

196 dimethylamine, or monomethylamine by various Methanosarcina species possesses one naturally occurring

197 vidence suggests that methanol catabolism in Methanosarcina species requires the concerted effort of

198 acetivorans were aligned with those in other Methanosarcina species to identify conserved transcripti

199 ency in methylamine metabolism expected of a Methanosarcina species unable to decode UAG codons as py

200 olving Ech hydrogenase complex of freshwater Methanosarcina species with the Rnf complex, which gener

201 region is well conserved among the sequenced Methanosarcina species, while the second vht-type homolo

202 tbp1 is strictly conserved in the genomes of Methanosarcina species.

203 Methanosarcina spp. begin methanogenesis from methylamin

204 is well conserved with respect to the other Methanosarcina spp. in the region proximal to the origin

205 dehydrogenase/acetyl coenzyme A synthase in Methanosarcina spp. is coordinately regulated in respons

206 sual possession of both LysRS1 and LysRS2 by Methanosarcina spp. may also reflect differences in cata

207 These vectors replicate in 9 of 11 Methanosarcina strains tested and in Escherichia coli.

208 tate kinases from E. coli (Bacteria domain), Methanosarcina thermophila (Archaea domain), and four ot

209 ototypic gamma-class carbonic anhydrase from Methanosarcina thermophila (Cam) were characterized by s

210 and E84A carbonic anhydrase from the archeon Methanosarcina thermophila (gamma class).

211 ure of a methanogenic CODH and CODH/ACS from Methanosarcina thermophila (MetCODH/ACS).

212 etermined the three-dimensional structure of Methanosarcina thermophila acetate kinase bound to ADP t

213 denosine pocket as compared to ATP-utilizing Methanosarcina thermophila ACK (MtACK).

214 , has been established in the methanoarchaea Methanosarcina thermophila and Methanococcus jannaschii.

215 two different members of the domain Archaea, Methanosarcina thermophila and Sulfolobus solfataricus.

216 e of nickel in this process two forms of the Methanosarcina thermophila beta subunit were overexpress

217 scribe the inhibition of acetate kinase from Methanosarcina thermophila by preincubation with MgCl(2)

218 cetate kinase is the homodimeric enzyme from Methanosarcina thermophila containing ADP and sulfate in

219 The CO dehydrogenase enzyme complex from Methanosarcina thermophila contains a corrinoid/iron-sul

220 HDR from Methanosarcina thermophila contains two b-hemes and two

221 Inspection of the crystal structure of the Methanosarcina thermophila enzyme containing only ADP re

222 recently determined crystal structure of the Methanosarcina thermophila enzyme identifies it as a mem

223 The enzyme from Methanosarcina thermophila has been cloned and hyper-pro

224 Isf (iron-sulfur flavoprotein) from Methanosarcina thermophila has been produced in Escheric

225 A catalytic mechanism for the enzyme from Methanosarcina thermophila has been proposed on the basi

226 ransacetylase from the methanogenic archaeon Methanosarcina thermophila in complex with the substrate

227 c analysis of the phosphotransacetylase from Methanosarcina thermophila indicated that there is a ter

228 rogenase/acetyl-CoA synthase (CODH/ACS) from Methanosarcina thermophila is part of a five-subunit com

229 k, the reaction catalyzed by the enzyme from Methanosarcina thermophila is shown to be analogous to o

230 The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma

231 taining carbonic anhydrase from the archaeon Methanosarcina thermophila suggests that a very similar

232 nic anhydrase from the thermophilic archaeon Methanosarcina thermophila that exhibits no significant

233 zyme complex was isolated from acetate-grown Methanosarcina thermophila that oxidized CO and catalyze

234 ataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryar

235 and sequenced from the methanogenic archaeon Methanosarcina thermophila TM-1.

236 way for the biosynthesis of methanopterin in Methanosarcina thermophila to proceed by the following s

237 oding an iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila was cloned and sequenced.

238 ed against the gamma carbonic anhydrase from Methanosarcina thermophila was detected.

239 The gene encoding carbonic anhydrase from Methanosarcina thermophila was hyperexpressed in Escheri

240 e catalytic mechanism of acetate kinase from Methanosarcina thermophila was investigated by diethylpy

241 The 20S proteasome from the methanoarchaeon Methanosarcina thermophila was produced in Escherichia c

242 The heterodisulfide reductase (HDR) from Methanosarcina thermophila was purified to homogeneity f

243 the prototypic gamma-carbonic anhydrase from Methanosarcina thermophila were characterized by extende

244 yl-coenzyme synthase (CODH/ACS) complex from Methanosarcina thermophila were cloned and sequenced.

245 s of the iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila were identified in databases.

246 site A-cluster in the ACDS beta subunit from Methanosarcina thermophila were investigated.

247 bonic anhydrase Cam (carbonic anhydrase from Methanosarcina thermophila) was investigated.

248 systems from Clostridium thermoaceticum and Methanosarcina thermophila).

249 characterization of OASS from acetate-grown Methanosarcina thermophila, a moderately thermophilic me

250 t A cluster proteins, ACDS beta subunit from Methanosarcina thermophila, acetyl-CoA synthase of Carbo

251 ichia coli of the phosphotransacetylase from Methanosarcina thermophila, an obligately anaerobic meth

252 purified from the methane-producing archaeon Methanosarcina thermophila, and the N-terminal sequence

253 Cam, from the procaryote Methanosarcina thermophila, is the prototype of the gamm

254 Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Meth

255 flavoprotein (Isf) from the methanoarchaeaon Methanosarcina thermophila, which participates in electr

256 also found in the methanogenic CODH/ACS from Methanosarcina thermophila.

257 by using cell extracts of M. jannaschii and Methanosarcina thermophila.

258 estigated for the phosphotransacetylase from Methanosarcina thermophila.

259 characterized from the methanogenic archaeon Methanosarcina thermophila.

260 osphate) and biotin, has been established in Methanosarcina thermophila.

261 ive form of methyl-SCoM reductase (MCR) from Methanosarcina thermophila.

262 se II, and the gamma-carbonic anhydrase from Methanosarcina thermophilain an effort to outline common

263 Because there are no known Methanosarcina transposons we modified the mariner trans

264 increase to 65 degrees C resulted in loss of Methanosarcina, with an accumulation of organic acids an