ライフサイエンスコーパス: NF45

1 we examined the cellular nuclear factor 45 (NF45).

2 hesis that this pathway is regulated by NF90/NF45.

3 sion, exists as a heterodimeric complex with NF45.

4 teins together with the less-studied protein NF45.

5 ut not of NF110 greatly reduces the level of NF45.

6 he NF90 proteins, including NF110, NF90, and NF45.

7 at in IBDV-infected cells the mainly nuclear NF45 accumulated at the sites of viral replication in th

8 NF90.NF45 and M phase phosphoprotein 4 belong to a large grou

9 show that heterodimeric core complexes, NF90-NF45 and NF110-NF45, exist within larger complexes that

10 NF45 and NF90 are enriched in nucleoli and cosediment wi

11 Together, these data indicate that NF45 and NF90 are novel higher-eukaryote-specific factor

12 m affinity purification, we identified human NF45 and NF90 as components of precursors to 60S (pre-60

13 Collectively, our results identify NF45 and NF90 as novel regulators of HS4-dependent human

14 binding domains of NF90, while depletion of NF45 and NF90 by RNA interference leads to a defect in 6

15 Nucleoli of cells depleted of NF45 and NF90 have altered morphology and display a char

16 of 60S subunit biogenesis, downregulation of NF45 and NF90 leads to a p53 response, accompanied by in

17 NA-binding subunits were identified as NF90, NF45 and systemic lupus erythematosis autoantigens, Ku80

18 dicated protein-protein associations between NF45 and VP1, VP2, and VP3.

19 sitely dependent on the levels of endogenous NF45 (and to a lesser degree NF90), because HS4-dependen

20 e interleukin enhancer binding factors ILF2 (NF45) and ILF3 (NF90/NF110) have been implicated in vari

21 mplementary DNAs encoding nuclear factor 45 (NF45) and NF90 were used to generate (35)S-methionine-la

22 M, hnRNP H, hnRNP C, Matrin3, NF110/NFAR-2, NF45, and DDX5, all approximately equimolar to Rbfox.

23 associated regulatory proteins p68, hnRNPA1, NF45, and NF90 in nuclei of PDAC and other tumor cells.

24 A granule assembly and disassembly by NFAR2, NF45, and phosphorylation at double-stranded RNA-activat

25 ecipitation analysis demonstrates that NF90, NF45, and PKR form a complex in both nuclear and cytosol

26 dues on NF90 that make up its interface with NF45 are conserved in two related proteins, spermatid pe

27 NF90 and NF45 are known to interact with the DNA-dependent protei

28 howed that NF90 and its heteromeric partner, NF45, are predominantly nuclear and largely chromatin-as

29 he promoter context, and suggests a role for NF45 as a regulator of NF90.

30 This study identified NF45 as an unstable regulatory subunit of NF90-NF45 comp

31 dentified in complex with nuclear factor 45 (NF45) as a sequence-specific DNA-binding protein, NF90 c

32 ulation and bound nuclear factor (NF) 90 and NF45, as demonstrated by DNA affinity chromatography cou

33 Transfection assays showed that NF45 binds NF90 strongly and stimulates its ability to a

34 ic nuclear factor (NF) 90/NF45 complex (NF90/NF45) binds nucleic acids and is a multifunctional regul

35 Knockdown of either NF90 or NF45 by RNA interference led to greatly elevated levels

36 ease of virus yield, our study suggests that NF45, by association with viral proteins, is part of a y

37 t IL13 expression was virtually abrogated in NF45(+/-) cells and reduced in NF90(+/-) cells.

38 The heterodimeric nuclear factor (NF) 90/NF45 complex (NF90/NF45) binds nucleic acids and is a mu

39 These results identify the NF90/NF45 complex as a regulator of DNA damage repair mediate

40 isk strains of HPV utilize the cellular NF90/NF45 complex for viral E6 expression in infected cervica

41 eviously reported that depletion of the NF90/NF45 complex results in a multinucleated phenotype.

42 45 as an unstable regulatory subunit of NF90-NF45 complexes and uncovered their critical role in norm

43 Depletion of NF90-NF45 complexes retards cell growth by inhibition of DNA

44 In vivo, NF90/NF45-depleted cells displayed increased gamma-histone 2A

45 Consistent with p53 derepression, NF90/NF45-depleted HeLa cells displayed elevated poly ADP-rib

46 We present the crystal structure of the NF90/NF45 dimerization complex at 1.9-A resolution.

47 NF90 and NF45 dimerize through their common 'DZF' domain (domain

48 odimeric core complexes, NF90-NF45 and NF110-NF45, exist within larger complexes that are more labile

49 Nuclear factors NF90 and NF45 form a complex involved in a variety of cellular pr

50 Nuclear factors 90 and 45 (NF90 and NF45) form a protein complex involved in the post-transc

51 Interference with NF90/NF45 function could assist in controlling cervical carci

52 Interference with NF90/NF45 function could assist in controlling cervical carci

53 However, both NF90 and NF45 have lost critical catalytic residues during evolut

54 The DRBP76:NF45 heterodimer binds to the HRV2 IRES in neuronal but

55 esults of this study suggest that the DRBP76:NF45 heterodimer selectively blocks HRV2 IRES-driven tra

56 olves the ILF3 complex (NF90 and its partner NF45/ILF2).

57 system, DNA end joining was reduced by NF90/NF45 immunodepletion or by RNA digestion to an extent si

58 arly promoter, revealing a new role for NF90/NF45 in HPV gene expression.

59 nuclear factor of activated T cells, 45 kDa (NF45), in neuronal but not in glioma cells.

60 Coregulation of NF90 and NF45 is a posttranscriptional phenomenon, resulting from

61 h the same binding interface, revealing that NF45 is able to form a variety of cellular complexes wit

62 d site-specific mutants, we demonstrate that NF45 is also able to recognize SPNR and Zfr through the

63 Further, NF90/NF45 knockdown reduced end-joining activity in vivo.

64 electron microscopy, we generated a model of NF45-NF90 complex formation on dsRNA.

65 This property of the NF45-NF90 complex has important implications for how lon

66 r data reveal that different interactions of NF45-NF90 complexes allow these proteins to coat long st

67 NF45-NF90 complexes recognize double-stranded RNA (dsRNA

68 We examined domain reorganization of NF45-NF90 domains on dsRNAs exceeding 50 bp to gain insi

69 Complexes of nuclear factors 45 and 90 (NF45-NF90) play a multitude of roles in co- and post-tra

70 p45 and p90 were identified as the NF45.NF90 complex, which binds the interleukin-2 promote

71 Consistent with a role of the NF45/NF90 heterodimer in nucleolar steps of 60S subunit

72 We show that association of the NF45/NF90 heterodimer with pre-60S ribosomal particles r

73 Notably, the CTGTT NF45/NF90-binding motif within HS4-3' was critical for H

74 ched by constrictions accumulate when either NF45 or NF90, but not NF110, is depleted.

75 tro differentiated Th2 cells from wild-type, NF45(+/-), or NF90(+/-) mice showed that HS4 activity wa

76 Besides binding dsRNA, NF90.NF45, p110, and p130 had single-stranded and dsDNA bindi

77 th dsRNA 2 (NFAR2), and we demonstrated that NF45 promotes disassembly of RNA granules, whereas NFAR2

78 sent in cellular complexes together with the NF45 protein.

79 tivated kinase sites and by association with NF45, respectively.

80 Here we report that depletion of NF90/NF45 restores the expression of the p53 and p21 proteins

81 fering RNA(siRNA)-mediated downregulation of NF45 resulted in an approximately 5-fold increase of vir

82 Depletion of the NF45 subunit by RNA interference is accompanied by a dra

83 sting of Mr = 90 000 (NF90) and Mr = 45 000 (NF45) subunits.

84 at ADAR1 is associated with NF110, NF90, and NF45 through the bridge of cellular dsRNA.

85 NF45 was previously indicated to be involved in the repl

86 NF45 was shown to specifically colocalize with the viral

87 t were identified, 4 (RACK1, Ku70, RPS3, and NF45) were expressed in rabbit reticulocyte lysate, bact

88 2 became dissociated from nuclear factor 45 (NF45), which was requisite for NFAR reshuttling, causing

89 ey copurified with the smaller NFAT subunit, NF45, which did not bind VA RNAII, and with an unidentif

90 not result in a cytoplasmic accumulation of NF45, which, among other data, showed that recruitment o