1 LF3 is closely related to nuclear factor 90 (
NF90).
2 , which was rescued by ectopic expression of
NF90.
3 d suggests a role for NF45 as a regulator of
NF90.
4 ated at least in part by the RNA-BPs HuR and
NF90.
5 es that are more labile and contain multiple
NF90/
110 isoforms and additional proteins.
6 ments, we discovered that nuclear factor 90 (
NF90),
a cellular RNA binding protein, regulates P-TEFb
7 Here, we report that nuclear factor 90 (
NF90),
a double-stranded RNA (dsRNA) binding protein, re
8 stranded RNA-binding protein 76/NF90 (DRBP76/
NF90),
a specific isoform of the DRBP family, as a VEGF
9 oteins were identified as RNA helicase A and
NF90,
a component of the heterodimeric nuclear factor of
10 NF90,
a previously described RNA binding protein, binds
11 In
NF90,
a tandem pair of dsRBDs separated by a natively un
12 et mRNAs was not significantly influenced by
NF90 abundance, while their translation increased when N
13 Conversely,
NF90 activated the cytomegalovirus immediate-early promo
14 Knockdown of
NF90 also inhibited tumor growth of HCC cell lines in mo
15 Nuclear factor 90 (
NF90),
an RNA-binding protein implicated in the regulati
16 minor groove of dsRNA are conserved between
NF90 and ADAR2.
17 and found a significant correlation between
NF90 and cyclin E1 expression in HCC.
18 equires the posttranscriptional induction of
NF90 and cyclin T1, implicating NF90 in protein kinase C
19 d the association of MKP-1 mRNA with HuR and
NF90 and decreased its association with the translationa
20 We identified cyclin E1 as a new target of
NF90 and found a significant correlation between NF90 an
21 Cell fractionation studies showed that
NF90 and its heteromeric partner, NF45, are predominantl
22 erminal loop, and involves the ILF3 complex (
NF90 and its partner NF45/ILF2).
23 We conclude that
NF90 and NF110 engage RNA differentially and translocate
24 estigated the mechanism of translocation for
NF90 and NF110, its larger splice variant.
25 nied by a dramatic decrease in the levels of
NF90 and NF110.
26 rding to ion type and membrane type (Filmtec
NF90 and NF270).
27 NF90 and NF45 are known to interact with the DNA-depende
28 NF90 and NF45 dimerize through their common 'DZF' domain
29 Nuclear factors
NF90 and NF45 form a complex involved in a variety of ce
30 However, both
NF90 and NF45 have lost critical catalytic residues duri
31 Coregulation of
NF90 and NF45 is a posttranscriptional phenomenon, resul
32 Nuclear factors 90 and 45 (
NF90 and NF45) form a protein complex involved in the po
33 NF90 and splice variant NF110/ILF3/NFAR are double-stran
34 interaction occurs between the N terminus of
NF90 and the C-terminal region of PKR, and an RNA-depend
35 NF90(+/+)-
and NF90(-/-)-RAG chimeric mice showed grossl
36 Nuclear factor 90 (
NF90)
and its C-terminally extended isoform, NF110, have
37 nuclear extracts consisting of Mr = 90 000 (
NF90)
and Mr = 45 000 (NF45) subunits.
38 hy), as well as an in vitro antiviral model (
NF90),
and healthy pediatric controls.
39 nstrate that ADAR1 is associated with NF110,
NF90,
and NF45 through the bridge of cellular dsRNA.
40 ing with the NF90 proteins, including NF110,
NF90,
and NF45.
41 er, pristane immunization still induced anti-
NF90 antibodies in granzyme B-deficient mice.
42 NF45 and
NF90 are enriched in nucleoli and cosediment with pre-60
43 Although ILF3 and
NF90 are highly similar, they differ in their carboxyl-t
44 d with wild-type cells, T cells deficient in
NF90 are impaired in ARRE and IL-2 transcriptional activ
45 We conclude that hnRNP and
NF90 are important host factors for HCV replication, at
46 Together, these data indicate that NF45 and
NF90 are novel higher-eukaryote-specific factors require
47 nism of cyclin T1 regulation and establishes
NF90 as a regulator of HIV-1 replication during both pro
48 This report characterizes
NF90 as both a positive and negative regulator of gene e
49 These data identify RNA helicase A and
NF90 as cellular proteins with an affinity for dsRNA and
50 y purification, we identified human NF45 and
NF90 as components of precursors to 60S (pre-60S) riboso
51 Collectively, our results identify NF45 and
NF90 as novel regulators of HS4-dependent human IL13 tra
52 randed RNA binding protein 76 (DRBP76/NFAR-1/
NF90)
as a cellular factor that associates with the VP35
53 ation analysis to identify a large subset of
NF90-
associated mRNAs.
54 ent small RNA genes, including snaR-A (small
NF90-
associated RNA isoform A), a hominid-specific nonco
55 ecipitation, we discovered a family of small
NF90-
associated RNAs (snaR).
56 Indeed, depletion of
NF90 attenuated the expression of E6 RNA and inhibited t
57 s of endogenous NF45 (and to a lesser degree
NF90),
because HS4-dependent IL13 expression was virtual
58 uring mitosis, about half of the cytoplasmic
NF90 becomes dissociated from RNA, but phosphorylation d
59 Ku80, Ku70 and
NF90 bind specifically to the IL-2 gene promoter in vivo
60 Notably, the CTGTT NF45/
NF90-
binding motif within HS4-3' was critical for HS4-de
61 NF90 binds to PKR through two independent mechanisms: an
62 single-stranded RNA, while DHX9, ADAR1, and
NF90 bound a cognate double-stranded RNA bait.
63 Furthermore,
NF90 bound to the 3' untranslated regions (3'UTRs) of cy
64 Reciprocally, depletion of
NF90 but not of NF110 greatly reduces the level of NF45.
65 constrictions accumulate when either NF45 or
NF90,
but not NF110, is depleted.
66 membrane performance were also observed for
NF90,
BW30, and NF270 membranes.
67 domains of NF90, while depletion of NF45 and
NF90 by RNA interference leads to a defect in 60S biogen
68 Suppression of
NF90 caused a decrease in the half-life of cyclin E1 mRN
69 abrogated in NF45(+/-) cells and reduced in
NF90(+/-)
cells.
70 ron microscopy, we generated a model of NF45-
NF90 complex formation on dsRNA.
71 This property of the NF45-
NF90 complex has important implications for how long, nu
72 p45 and p90 were identified as the NF45.
NF90 complex, which binds the interleukin-2 promoter as
73 a reveal that different interactions of NF45-
NF90 complexes allow these proteins to coat long stretch
74 NF45-
NF90 complexes recognize double-stranded RNA (dsRNA) and
75 Both RNA helicase A and
NF90 contain two copies of a double-stranded (ds) RNA bi
76 as a sequence-specific DNA-binding protein,
NF90 contains two double stranded RNA-binding motifs (ds
77 ted in HCC specimens and that suppression of
NF90 decreases HCC cell growth and delays G1/S transitio
78 -NF90m mRNA was specifically repressed in an
NF90-
dependent manner, as determined by analysing nascen
79 NF90 depletion reduced cyclin T1 protein levels by inhib
80 iption elongation factor b) levels caused by
NF90 depletion.
81 Because of this difference,
NF90 does not interact with PRMT1, is a much poorer subs
82 We examined domain reorganization of NF45-
NF90 domains on dsRNAs exceeding 50 bp to gain insight i
83 ified double-stranded RNA-binding protein 76/
NF90 (
DRBP76/NF90), a specific isoform of the DRBP famil
84 gene expression through interacting with the
NF90 family proteins.
85 Members of the nuclear factor 90 (
NF90)
family of double-stranded RNA (dsRNA)-binding prot
86 Members of the nuclear factor 90 (
NF90)
family of human double-stranded RNA (dsRNA) bindin
87 NF90-
Gal4 fusion proteins inhibited transcription from t
88 NF90 gene-targeted mice exhibit perinatal lethality.
89 Fetal liver cells from
NF90 gene-targeted mice were transplanted into irradiate
90 NF90 has additional double-stranded RNA-binding domains
91 These results suggest that
NF90 has an important role in HCC pathogenesis and that
92 The RNA-binding protein nuclear factor 90 (
NF90)
has been implicated in the stabilization, transpor
93 Nucleoli of cells depleted of NF45 and
NF90 have altered morphology and display a characteristi
94 Consistent with a role of the NF45/
NF90 heterodimer in nucleolar steps of 60S subunit bioge
95 We show that association of the NF45/
NF90 heterodimer with pre-60S ribosomal particles requir
96 including IGF2BP1, hnRNP L, DHX9, ADAR1, and
NF90 (
ILF3).
97 ssociate in vivo with the nuclear factor 90 (
NF90/
ILF3) protein.
98 a crystal structure of the tandem dsRBDs of
NF90 in complex with a synthetic dsRNA.
99 Taken together, downregulation of
NF90 in HCC cell lines can delay cell-cycle progression,
100 aracterize the transcriptional properties of
NF90 in mammalian cells, we examined its ability to modu
101 Stable repression of DRBP76/
NF90 in MDA-MB-435 breast cancer cells demonstrated redu
102 Our results support a role for
NF90 in modulating key genes implicated in the immune re
103 regulatory proteins p68, hnRNPA1, NF45, and
NF90 in nuclei of PDAC and other tumor cells.
104 induction of NF90 and cyclin T1, implicating
NF90 in protein kinase C signaling pathways.
105 NF90 interacts with RNAs in the following order of affin
106 NF90 is a member of the dsRNA binding domain (dsRBD) fam
107 Furthermore, the study revealed that
NF90 is functionally distinct from NF110 and is more imp
108 In nonstimulated cells,
NF90 is mostly nuclear, but T cell activation results in
109 NF90 is phosphorylated by PKR in its RNA-binding domain,
110 During interphase,
NF90 is predominantly nuclear, NF110 is exclusively nucl
111 NF90 is present in cellular complexes together with the
112 The nuclear export of
NF90 is required for IL-2 mRNA stabilization.
113 About half of the
NF90 is tethered in the nucleus by RNA bound to the prot
114 Northwestern blotting revealed that
NF90 is the principal and specific p21WAF1/CIP1 and MyoD
115 NF90 is uniquely cleaved by granzyme B in vitro; however
116 We demonstrate that
NF90 is upregulated in HCC specimens and that suppressio
117 Nuclear factor 90 (
NF90)
is a double-stranded RNA-binding protein implicate
118 Nuclear factor 90 (
NF90)
is a member of an expanding family of double-stran
119 Nuclear Factor 90 (
NF90)
is an RBP encoded by the interleukin enhancer-bind
120 These data demonstrate that the DRBP76/
NF90 isoform facilitates VEGF expression by promoting VE
121 RNA interference targeting the DRBP76/
NF90 isoform limited hypoxia-inducible VEGF mRNA and pro
122 Cells with stable repression of the DRBP76/
NF90 isoform showed reduced tumorigenic and angiogenic p
123 Both NF110 isoforms are more active than
NF90 isoforms in stimulating transcription from the prol
124 levels of cyclin E1 were downregulated upon
NF90 knockdown.
125 bunit biogenesis, downregulation of NF45 and
NF90 leads to a p53 response, accompanied by induction o
126 ance, while their translation increased when
NF90 levels were reduced.
127 ADAR1 upregulates
NF90-
mediated gene expression by interacting with the NF
128 e complete function of ADAR1 in upregulating
NF90-
mediated gene expression.
129 Furthermore, we demonstrate that an
NF90 membrane is capable of removing 99.5% of HFPO-DA fr
130 Compared with newborn
NF90(+/+)
mice, newborn NF90(-/-) mice demonstrate sever
131 ated Th2 cells from wild-type, NF45(+/-), or
NF90(+/-)
mice showed that HS4 activity was exquisitely
132 ompared with newborn NF90(+/+) mice, newborn
NF90(-/-)
mice demonstrate severe impairment of IL-2 exp
133 The diaphragm and other skeletal muscles in
NF90(-/-)
mice demonstrated disorganized arrangement and
134 NF90(-/-)
mice were born small and weak and succumbed to
135 Lung inflation and morphology were normal in
NF90(-/-)
mice.
136 and p21WAF1/CIP1, was severely decreased in
NF90(-/-)
mice.
137 We hypothesized that
NF90 modulates the activity of DNA-PK.
138 Insertion of the AU-rich
NF90 motif ('NF90m') in the 3'UTR of an EGFP heterologou
139 ombinant biotinylated transcripts containing
NF90 motif hits.
140 nd this reaction is partially blocked by the
NF90 N-terminal region.
141 In this study,
NF90 nanofiltration membrane was treated with sodium hyp
142 hancer binding factors ILF2 (NF45) and ILF3 (
NF90/
NF110) have been implicated in various cellular pat
143 ARRE DNA-binding subunits were identified as
NF90,
NF45 and systemic lupus erythematosis autoantigens
144 munoprecipitation analysis demonstrates that
NF90,
NF45, and PKR form a complex in both nuclear and c
145 We show that heterodimeric core complexes,
NF90-
NF45 and NF110-NF45, exist within larger complexes
146 ed NF45 as an unstable regulatory subunit of
NF90-
NF45 complexes and uncovered their critical role in
147 Depletion of
NF90-
NF45 complexes retards cell growth by inhibition of
148 NF90.
NF45 and M phase phosphoprotein 4 belong to a large
149 Besides binding dsRNA,
NF90.
NF45, p110, and p130 had single-stranded and dsDNA
150 These results identify the
NF90/
NF45 complex as a regulator of DNA damage repair me
151 igh-risk strains of HPV utilize the cellular
NF90/
NF45 complex for viral E6 expression in infected ce
152 We previously reported that depletion of the
NF90/
NF45 complex results in a multinucleated phenotype.
153 We present the crystal structure of the
NF90/
NF45 dimerization complex at 1.9-A resolution.
154 Interference with
NF90/
NF45 function could assist in controlling cervical
155 Interference with
NF90/
NF45 function could assist in controlling cervical
156 assay system, DNA end joining was reduced by
NF90/
NF45 immunodepletion or by RNA digestion to an exte
157 HPV early promoter, revealing a new role for
NF90/
NF45 in HPV gene expression.
158 Further,
NF90/
NF45 knockdown reduced end-joining activity in vivo
159 Here we report that depletion of
NF90/
NF45 restores the expression of the p53 and p21 pro
160 dimeric nuclear factor (NF) 90/NF45 complex (
NF90/
NF45) binds nucleic acids and is a multifunctional
161 In vivo,
NF90/
NF45-depleted cells displayed increased gamma-histo
162 Consistent with p53 derepression,
NF90/
NF45-depleted HeLa cells displayed elevated poly AD
163 hypothesis that this pathway is regulated by
NF90/
NF45.
164 et of targets and investigated the impact of
NF90 on their expression levels.
165 es not impair the binding affinity of either
NF90 or NF110 for dsRNA.
166 Knockdown of either
NF90 or NF45 by RNA interference led to greatly elevated
167 A model for
NF90-
PKR interactions is proposed.
168 Complexes of nuclear factors 45 and 90 (NF45-
NF90)
play a multitude of roles in co- and post-transcri
169 The DRBP76/
NF90 protein binds to a human VEGF 3' untranslated mRNA
170 Transient overexpression of the DRBP76/
NF90 protein increased both VEGF mRNA and protein levels
171 The
NF90 protein was found to be expressed not only in T-cel
172 ated gene expression by interacting with the
NF90 proteins, including NF110, NF90, and NF45.
173 n by interacting with the nuclear factor 90 (
NF90)
proteins, known regulators that bind the antigen r
174 Compared with littermates,
NF90(-/-)
RAG chimeric mice exhibited profound T cell ly
175 NF90(+/+)- and
NF90(-/-)-
RAG chimeric mice showed grossly normal repopu
176 erlying sequences in dsRNA may influence how
NF90 recognizes its target RNAs.
177 Thus,
NF90 regulates inducible IL-2 transcription, mRNA stabil
178 NF90 regulates transcription factors and a cell cycle in
179 target mRNAs and have obtained evidence that
NF90 represses the translation of this subset of mRNAs.
180 It interacts with
NF90'
s double-stranded RNA-binding motifs.
181 Moreover, we showed that inhibition of
NF90 sensitized HCC cells to the cyclin-dependent kinase
182 fering RNA-mediated depletion of hnRNP L and
NF90 significantly impaired viral replication and reduce
183 HuR or
NF90 silencing significantly diminished the H(2)O(2)-sti
184 The C-terminally extended
NF90 species, NF110, are almost exclusively chromatin-bo
185 ated polypeptides, we have demonstrated that
NF90 specifically binds to double-stranded RNA.
186 Monoclonal antibodies to Ku80, Ku70 and
NF90 specifically inhibit constitutive and inducible ARR
187 Transfection assays showed that NF45 binds
NF90 strongly and stimulates its ability to activate but
188 opulation of the thymus and spleen, but only
NF90(-/-)
T cells were severely impaired in IL-2 gene ex
189 e identified an AU-rich RNA motif present in
NF90 target mRNAs and have obtained evidence that NF90 r
190 However, a systematic analysis of
NF90 target mRNAs has not been performed.
191 ysis showed that the stability of endogenous
NF90 target mRNAs was not significantly influenced by NF
192 Residues on
NF90 that make up its interface with NF45 are conserved
193 tic testing of the TTR-RBPs AUF1, HuR, KSRP,
NF90,
TIA-1, and TIAR led to three key discoveries.
194 uence mRNA turnover and/or translation (HuR,
NF90,
TIAR, and TIA-1) were found to bind to biotinylate
195 Inducible binding of
NF90 to the IL-2 promoter in vivo is shown by chromatin
196 ary spleen cells induces binding of Ku80 and
NF90 to the IL-2 promoter in vivo, and decreases binding
197 Dynamic changes in binding of Ku80, Ku70 and
NF90 to the IL-2 proximal promoter in vivo correlate wit
198 ts of this heterodimer have been cloned, and
NF90 was found to encode a protein containing two domain
199 The double-stranded RNA-binding protein
NF90 was identified as a novel substrate for caspases an
200 Furthermore,
NF90 was phosphorylated in a double-stranded RNA-depende
201 Nuclear factor 90 (
NF90)
was originally isolated in a complex that binds to
202 tically identify the RNAs that interact with
NF90,
we carried out iCLIP (individual-nucleotide resolu
203 Mice with targeted disruption of
NF90 were engineered.
204 hnRNP L and
NF90 were found to associate with HCV RNA in infected ce
205 y DNAs encoding nuclear factor 45 (NF45) and
NF90 were used to generate (35)S-methionine-labeled prot
206 but overlapping domains in the C terminus of
NF90,
which contains a functional nuclear localization s
207 s the double-stranded RNA binding domains of
NF90,
while depletion of NF45 and NF90 by RNA interferen
208 A knockdown of
NF90 with small interfering RNA suppresses this function
209 The interaction of endogenous
NF90 with target mRNAs was validated after testing both
210 ly nuclear RNA-binding proteins, hnRNP L and
NF90,
with previously unrecognized proviral roles in HCV