ライフサイエンスコーパス: NRSF

1 NRSF binds to the NRSE of the MOR gene in a sequence-spe

2 NRSF is normally down-regulated upon neuronal differenti

3 NRSF/REST has been demonstrated to regulate at least 30

4 NRSF/REST is a protein that silences transcription of a

5 he human transcription factors SRF, GABP and NRSF at an average resolution of about 20 base pairs.

6 ng transcription factor (REST, also known as NRSF) is a master repressor of neuronal gene expression

7 ilencing transcription factor; also known as NRSF) to its cognate RE1 sequences is temporally regulat

8 lencer of transcription (REST; also known as NRSF) transcription factor.

9 ng transcription factor (REST, also known as NRSF).

10 uggest that the synergic interaction between NRSF and Sp3 is required to negatively regulate MOR gene

11 was used to demonstrate interaction between NRSF/REST and REST4.

12 Blocking NRSF transiently after eFSE prevented granule cell dysma

13 Many of these putative NRSEs bound NRSF in vitro and repressed transcription in vivo.

14 elix is reversed compared to that adopted by NRSF, a transcription factor unrelated to SAP25, upon bi

15 repression of the cholinergic gene locus by NRSF/REST.

16 Down-regulation of NRP1 by NRSF overexpression reduced Sema3A activity.

17 is dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional

18 by GNAO1, is transcriptionally regulated by NRSF and is increased in the ventricles of several mouse

19 by GNAO1, is transcriptionally regulated by NRSF and is increased in the ventricles of several mouse

20 Genes selectively regulated by NRSF during epileptogenesis coded for ion channels, rece

21 Unexpectedly, genes selectively repressed by NRSF had mid-range binding frequencies to the repressor,

22 isms involving transcriptional repression by NRSF.

23 cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can exp

24 The DNA-binding protein REST (also called NRSF) is a transcriptional repressor that targets many n

25 -silencing transcription factor; also called NRSF) is expressed at high levels in mouse embryonic ste

26 posite NRSE to identify additional candidate NRSF target genes.

27 his cis-activating NRSE element also confers NRSF-dependent modulation in the context of the native p

28 a DNA-protein complex with NRSF, and confers NRSF-dependent transcriptional repression in the context

29 Although nuclear extracts containing NRSF/REST from A126.1B2 exhibited binding to NRSE/RE-1,

30 814, 5813 and 73 956 binding sites for CTCF, NRSF and STAT1 proteins, respectively, which is 32, 299

31 targeted mutation of Rest, the gene encoding NRSF, caused derepression of neuron-specific tubulin in

32 In the co-immunoprecipitation experiment, NRSF interacted with the full-length Sp3 factor, but not

33 fferentiated while constitutively expressing NRSF showed a significantly increased frequency of axon

34 The neuron-restrictive silencer factor NRSF (also known as REST and XBR) can silence transcript

35 sion is mediated by the transcription factor NRSF, which recruits the NADH-binding co-repressor CtBP

36 d ChIP-seq data for the transcription factor NRSF/REST, a study of ChIP-seq analysis with or without

37 ending on neuron-restriction silence factor (NRSF) expression levels.

38 that the neuron restrictive silencer factor (NRSF) binds to NRSE.

39 r (REST)/neuron-restrictive silencer factor (NRSF) can repress several terminal neuronal differentiat

40 r (REST)/neuron-restrictive silencer factor (NRSF) can repress transcription of a battery of neuronal

41 et al.'s neuron-restrictive silencer factor (NRSF) ChIP-Seq data without relying on extensive qPCR va

42 r (REST)/neuron-restrictive silencer factor (NRSF) plays a critical role in elaboration of the neuron

43 The neuron-restrictive silencer factor (NRSF) represses transcription of several neuronal genes

44 gulator, neuron restrictive silencer factor (NRSF), and its downstream target genes.

45 ulators, neuron-restrictive silencer factor (NRSF), has been shown to repress the expression of neuro

46 known as Neuron-Restrictive Silencer Factor (NRSF), is a key regulator of this process.

47 epressor neuron-restrictive silencer factor (NRSF), which negatively regulates Crh gene transcription

48 Neuron-restrictive silencer factor (NRSF)/RE-1-silencing transcription factor (REST), the tr

49 n of the neuron-restrictive silencer factor (NRSF/REST), an important transcription factor that influ

50 The neuron-restrictive silencer factor (NRSF; also known as REST for repressor element-1 silenci

51 g of the neuron-restrictive silencer factor (NRSF; also known as REST, for repressor element-1 silenc

52 pressor neuron restrictive silencing factor (NRSF or REST).

53 factor/neuron restrictive silencing factor (NRSF).

54 ent-1 (RE-1) silencing transcription factor (NRSF/REST) contains nine zinc finger domains and binds t

55 The neuron-restrictive silencer factor [NRSF (RE-1 silencing transcription factor/X box represso

56 iption factors: CTCF (CCCTC-binding factor), NRSF (neuron-restrictive silencer factor) and STAT1 (sig

57 or element-1 silencing transcription factor)/NRSF (neuron-restrictive silencing factor) and REST-depe

58 r (REST; Neuron-Restrictive Silencer Factor, NRSF) as a predicted upstream suppressor of a pro-regene

59 known as neuron-restrictive silencer factor, NRSF) is a universal feature of normal ageing in human c

60 known as neuron restrictive silencer factor, NRSF).

61 known as neuron restrictive silencer factor, NRSF).

62 or element 1 silencing transcription factor]/NRSF (neuron-restrictive silencer factor) actively repre

63 ChIP-seq data for the transcription factors NRSF and GABP.

64 -factor genes, and these tested positive for NRSF/REST occupancy in vivo.

65 ession of the pax4 gene and infer a role for NRSF in pancreatic islet development.

66 ngs suggest an important functional role for NRSF in the expression of the pax4 gene and infer a role

67 Acquired HCN1 channelopathy derives from NRSF-mediated transcriptional repression that endures vi

68 he NRSE of the MOR promoter is functional in NRSF-positive cells (NS20Y and HeLa) but not in NRSF-neg

69 F-positive cells (NS20Y and HeLa) but not in NRSF-negative cells (PC12).

70 Increased NRSF binding to chromatin was accompanied by sequential

71 g neurons dissipated by adulthood, increased NRSF levels and repression of CRH expression persisted,

72 nvertebrate and protochordate genomes, as is NRSF itself.

73 pilepsy-provoking seizures increased the low NRSF levels in mature hippocampus several fold yet surpr

74 ng the transcription factor REST (also named NRSF or XBR).

75 RNA and protein, whereas a dominant negative NRSF increased NRP1.

76 either trichostatin A or a dominant-negative NRSF induced MOR promoter activity and transcription of

77 -specifically expressing a dominant-negative NRSF mutant.

78 tifs and allowed us to identify noncanonical NRSF-binding motifs.

79 hromatin immunoprecipitation-seq analyses of NRSF targets identified gene networks that, in addition

80 REST4 inhibited binding of NRSF/REST to NRSE/RE-1 as determined by gel mobility shi

81 Constitutive expression of NRSF in the developing spinal cord of chicken embryos ca

82 d them sensitive to moderate fluctuations of NRSF levels.

83 n embryos, using a dominant-negative form of NRSF, also caused derepression of neuronal tubulin, as w

84 REST4, a C-terminally truncated form of NRSF/REST, contains the five N-terminal zinc fingers and

85 Mosaic inhibition of NRSF in chicken embryos, using a dominant-negative form

86 se inhibitor trichostatin A, an inhibitor of NRSF silencing activity, also increased NRP1 levels.

87 Levels of NRSF and its physical binding to the Hcn1 gene were augm

88 Overexpression of NRSF in HaCaT cells decreased NRP1 RNA and protein, wher

89 PCR validated NRSF sites and the presence of NRSF binding motifs for setting thresholds.

90 These data suggest that down-regulation of NRSF is necessary for the proper development of at least

91 Thus, dynamic, selective regulation of NRSF target genes may play a role in influencing neurona

92 ression, concomitant with down-regulation of NRSF.

93 This provides direct evidence the role of NRSF in the cells and also indicates that NRSF expressio

94 Since silencing of NRSF is known to initiate neural differentiation, it sug

95 The suppression of NRSF activity with either trichostatin A or a dominant-n

96 d downregulation of Tbx3 and upregulation of NRSF and miR-1 (transcriptional regulators) that explain

97 sion in specific neuronal cells depending on NRSF expression level.

98 llers, including candidate feedback loops on NRSF and its corepressor, CoREST.

99 epressive epigenetic changes which outlasted NRSF binding.

100 y a subset ( approximately 10%) of potential NRSF target genes.

101 ement [NRSE]), in vitro and in vivo, reduced NRSF binding to Hcn1, prevented its repression, and rest

102 cally tested site for the neuronal repressor NRSF/REST, Cistematic generated a refined PSFM (position

103 Physical interaction of the repressor, NRSF, was abolished using decoy oligodeoxynucleotides (O

104 y reported that a transcriptional repressor, NRSF (neuron restrictive silencer factor), suppresses th

105 hat bind the transcriptional repressor REST (NRSF) encode in vivo DNA binding affinity hierarchies th

106 r RE-1-silencing transcription factor (REST)/NRSF, both the NRSE and sequences in the first intron we

107 REST/NRSF (repressor-element-1-silencing transcription factor

108 REST/NRSF acts as a regulator of neuron-specific gene express

109 REST/NRSF is a transcriptional repressor that acts at the ter

110 REST/NRSF is expressed most highly in non-neural tissues, whe

111 REST/NRSF is generally downregulated during induction of neur

112 REST/NRSF recruits CoREST and mSin3A corepressors to stem cel

113 REST/NRSF was first identified as a transcriptional repressor

114 To determine whether c-Myc and REST/NRSF act together to cause medulloblastomas, we used a p

115 sential link between H3K4 complexes and REST/NRSF and provide the first direct genetic evidence that

116 hese experiments show that the NRSE and REST/NRSF are important components in restricting L1 expressi

117 t fashion in both muscle-derived L6 and REST/NRSF co-transfected neuronal PC12 cells.

118 of neural progenitor proliferation, and REST/NRSF, a transcriptional repressor of neuronal differenti

119 nscription and recruits RBPJ/Sin3A- and REST/NRSF-repressive complexes to repress p14(ARF) and p16(IN

120 g the nuclear localization of REST4 and REST/NRSF.

121 trol by ARID1A, possibly through ARID1A-REST/NRSF interaction.

122 ereas Esco2 is infrequently enriched at REST/NRSF target genes.

123 te system showed an interaction between REST/NRSF and RILP as well as between RILP and dynactin p150(

124 ferase constructs was down-regulated by REST/NRSF in a RE-1/NRSE-dependent fashion in both muscle-der

125 sing hormone (CRH) gene is regulated by REST/NRSF, in part through the RE-1/NRSE.

126 long-term repression events mediated by REST/NRSF.

127 malian cell types and activate cellular REST/NRSF target genes, even in the absence of factors that a

128 al units that do not themselves contain REST/NRSF response elements.

129 into the nucleus and that HAP1 controls REST/NRSF cellular localization in neurons.

130 dulloblastoma cells was able to counter REST/NRSF-mediated repression of neuronal promoters, stimulat

131 In addition, countering REST/NRSF function blocked the tumorigenic potential of NSC-M

132 ds to the same DNA binding site as does REST/NRSF but functions as an activator instead of a represso

133 n did not interact directly with either REST/NRSF or RILP, but did interact with dynactin p150(Glued)

134 was able to compete with the endogenous REST/NRSF for DNA binding and stimulate neuronal promoters.

135 entiation pathway, countered endogenous REST/NRSF-dependent repression, activated the REST/NRSF targe

136 tor/neuron-restrictive silencer factor (REST/NRSF) at very high levels compared with either neuronal

137 r/neuronal restricted silencing factor (REST/NRSF) can mediate extraneuronal restriction by imposing

138 tor/neuron-restrictive silencer factor (REST/NRSF) complex.

139 ion/neuron-restrictive silencer factor (REST/NRSF) controls hundreds of neuron-specific genes.

140 or/neuron-restrictive silencing factor (REST/NRSF) in many neuron-specific genes.

141 tor/Neuron-Restrictive Silencer Factor (REST/NRSF) is a gene-silencing factor that is widely expresse

142 tor/neuron-restrictive silencer factor (REST/NRSF) silences neuronal genes in neural stem cells (NSCs

143 or/neuron-restrictive silencing factor (REST/NRSF) target genes activate UL growth due to the near ub

144 or/neuron-restrictive silencing factor (REST/NRSF), a known tumor suppressor, transcriptionally repre

145 ion/neuron-restrictive silencer factor (REST/NRSF)--thought to regulate hundreds of neuron-specific g

146 the RE1-silencing transcription factor (REST/NRSF).

147 tor/neuron-restrictive silencer factor (REST/NRSF).

148 nown neural restrictive silencer factor REST/NRSF.

149 ions identify the transcription factors REST/NRSF and FOXOs as critical for the diapause gene express

150 evealed seemingly paradoxical roles for REST/NRSF in neurogenesis, neural plasticity, tumour suppress

151 ion in PRICKLE1 (also known as RILP for REST/NRSF interacting LIM domain protein) in all three of the

152 unrecognized tumor suppressor role for REST/NRSF, a transcriptional repressor of neuronal gene expre

153 aller set of genes, including those for REST/NRSF, Groucho, nucleophosmin, and Ubc4/5E2.

154 ears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors.

155 ndent genes contained binding sites for REST/NRSF, suggesting that release from general repression in

156 We propose the name RILP, for REST/NRSF-interacting LIM domain protein, to label this novel

157 ene (kaposin A)-mediated decreased host REST/NRSF (RE1-silencing transcription factor/neuron-restrict

158 However, REST/NRSF(-/-) mice suggest that the absence of REST/NRSF-dep

159 Our work identifies REST/NRSF as a master negative regulator of adult NSC differe

160 ere, we show that hyperactivity-induced REST/NRSF activation, triggers a homeostatic rearrangement of

161 Moreover, mice lacking REST/NRSF specifically in NSCs display a transient increase i

162 In another neuronal cell line, NG108, REST/NRSF also repressed expression from constructs containin

163 udies have shown that neither c-Myc nor REST/NRSF alone could cause tumor formation.

164 gs indicate that abnormal expression of REST/NRSF and Myc in NSCs causes cerebellum-specific tumors b

165 e shown that heterologous expression of REST/NRSF in Saccharomyces cerevisiae is able to repress tran

166 findings highlight the central role of REST/NRSF in the complex transcriptional responses aimed at r

167 omplex involved in the translocation of REST/NRSF into the nucleus and that HAP1 controls REST/NRSF c

168 implicated in the homeostatic action of REST/NRSF is unknown.

169 1/NRSE-mediated repressive influence of REST/NRSF is well established, results in transgenic studies

170 esults showed that direct activation of REST/NRSF target genes in NSCs with a single transgene, REST-

171 sults suggest that direct activation of REST/NRSF target genes with a single transgene, REST-VP16, is

172 directly activate the transcription of REST/NRSF target genes.

173 VP16, by replacing repressor domains of REST/NRSF with the activation domain of a viral activator VP1

174 ucted by replacing repressor domains of REST/NRSF with the activation domain of viral protein (VP16).

175 To counter the effect of REST/NRSF, we used a recombinant transcription factor, REST-V

176 F(-/-) mice suggest that the absence of REST/NRSF-dependent repression alone is not sufficient for th

177 the N-terminal Sin3p binding domain of REST/NRSF.

178 cells mediated by the repressor protein REST/NRSF (RE1 silencing transcription factor/neural-restrict

179 Its cognate binding protein, REST/NRSF, is an essential transcription factor; its null mut

180 (RILP) has also been shown to regulate REST/NRSF nuclear translocation.

181 ered to express a doxycycline-regulated REST/NRSF transgene (NSC-M-R), they no longer underwent termi

182 ethylation complex, directly regulating REST/NRSF, a master regulator of neural gene expression and c

183 8 may associate with another regulator, REST/NRSF, predominately at promoter regions via studying sev

184 The transcriptional repressor REST/NRSF (RE-1 silencing transcription factor/neuron-restric

185 tudies of the transcriptional repressor REST/NRSF (RE1 Silencing Transcription Factor or Neural Restr

186 The neuronal gene repressor REST/NRSF recruits corepressors, including CoREST, to modify

187 The repressor REST/NRSF restricts expression of a large set of genes to neu

188 We show that REST/NRSF binding to RE1/NRSE is accompanied by a decrease in

189 Furthermore, we show that REST/NRSF binds mammalian SIN3A and HDAC-2 and requires histo

190 These results suggest that REST/NRSF can act as both a repressor of Crh transcription, v

191 We showed that REST/NRSF downregulates glutamatergic transmission in respons

192 Here we report that REST/NRSF is required to maintain the adult neural stem cell

193 res both yeast Sin3p and Rpd3p and that REST/NRSF physically interacts with the product of the yeast

194 We show that REST/NRSF recruits SCPs to neuronal genes that contain RE-1 e

195 Taken together, these data suggest that REST/NRSF represses neuronal gene transcription by recruiting

196 cells with trichostatin A revealed that REST/NRSF repression depends, in part, on histone deacetylase

197 Thus, this study suggests that REST/NRSF, dynactin p150(Glued), huntingtin, HAP1, and RILP f

198 er activity requires the binding of the REST/NRSF repressor complex in nonneuronal cells.

199 RSF-dependent repression, activated the REST/NRSF target genes, and, surprisingly, activated other ne

200 The REST/NRSF-interacting LIM domain protein (RILP) has also been

201 Therefore, REST/NRSF may serve as a new target for therapeutic intervent

202 the same RE1/NRSE, but activates these REST/NRSF target genes.

203 revents this complex from translocating REST/NRSF to the nucleus.

204 However, unexpectedly, REST/NRSF up-regulated expression levels of constructs lackin

205 se-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P1

206 ecruits the histone methylase G9a to silence NRSF target genes in nonneuronal cells.

207 Some NRSF/REST sites reside in repeats, which suggests a mech

208 We generated cardiac-specific NRSF knockout mice and analyzed cardiac gene expression

209 scription-associated factors, including SRF, NRSF, GABP, Stat3 and p300 in different developmental co

210 g of the repressor protein REST (also termed NRSF or XBR) to the RE1 (also called NRSE) sequence in t

211 Analysis of two well-studied TFs, NRSF and CCCTC-binding factor (CTCF), also suggests that

212 It was concluded that NRSF is a transcription factor that silences NRP1 expres

213 in several nonneuronal genes, implying that NRSF may play a broader role than originally anticipated

214 These results indicate that NRSF is required to repress neuronal gene expression in

215 of NRSF in the cells and also indicates that NRSF expression is regulated by post-translational modif

216 Here we report that NRSF recruits the histone methylase G9a to silence NRSF

217 Our data suggested that NRSF can function as a repressor of MOR transcription in

218 factor genes contain NRSEs, suggesting that NRSF may repress neuronal differentiation both directly

219 The NRSF splice variant represents a specific clinical marke

220 The NRSF/REST isoform REST4 was expressed in PC12 cells but

221 Administration of decoy ODNs comprising the NRSF DNA-binding sequence (neuron restrictive silencer e

222 ent with NRSE in NS20Y cells, but not in the NRSF negative PC12 cells.

223 re we describe a novel splice variant of the NRSF transcript, which is highly expressed in SCLCs.

224 When the NRSF was disrupted in NS20Y and HeLa cells using small i

225 we have identified core residues within the NRSF and CTCF binding sites that are critical for a stro

226 Thus, up-regulated expression of this NRSF isoform may be a key early factor in defining the n

227 on and predicting translation of a truncated NRSF isoform.

228 without relying on extensive qPCR validated NRSF sites and the presence of NRSF binding motifs for s

229 gly, the repressed gene-set was rescued when NRSF binding to chromatin was blocked.

230 m to elucidate molecular mechanisms by which NRSF maintains normal cardiac function.

231 evolution, forms a DNA-protein complex with NRSF, and confers NRSF-dependent transcriptional repress

232 uron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the tr