ライフサイエンスコーパス: NatA

1 NatA co-translationally acetylates the N termini of a wi

2 NatA is bound to Huntingtin-interacting protein K (HYPK)

3 Among them, N-terminal acetyltransferase A (NatA) acetylates the N terminus of ~40% of the eukaryoti

4 bunit in the N-terminal acetyltransferase A (NatA) complex.

5 peptidase (MetAP) and N-acetyltransferase A (NatA), respectively(1).

6 HYPK accelerates NatA dissociation from the ribosome to license multiple

7 rprisingly, this enzyme is able to acetylate NatA and NatE substrates and is believed to represent an

8 The N-terminal acetyltransferase NatA is a heterodimeric complex consisting of a catalyti

9 talyzed by the N-terminal acetyltransferases NatA, NatB, and NatC.

10 alyzed by seven N(alpha)-acetyltransferases (NatA-F and NatH).

11 , and the three N(alpha)-acetyltransferases, NatA, NatB, and NatC, which collectively modify approxim

12 pes of N(alpha)-terminal acetyltransferases, NatA, NatB, and NatC, with each having a different catal

13 two-hybrid protein K(4,5) (HYPK) to activate NatA on the ribosome, enforcing cotranslational N-termin

14 referring basic and aromatic amino acids and NatA preferring acidic or polar residues.

15 embles a multienzyme complex with MetAP1 and NatA early during translation and pre-positions the acti

16 tionship between ADO-catalysed oxidation and NatA-catalysed acetylation of a broad range of protein s

17 ases significantly after drought stress, and NatA abundance is rapidly downregulated by the phytohorm

18 t each other's NAT activity in vitro Because NatA and Naa50 exhibit distinct substrate specificity, w

19 tress and that imprinting of the proteome by NatA is an important switch for the control of metabolis

20 -terminal acetyltransferase (NAT) complexes (NatA-NatC), which co-translationally acetylate the N-ter

21 The core NatA complex consists of the catalytic subunit NAA10 and

22 yeast (Saccharomyces cerevisiae), this core NatA complex interacts with NAA50 to form the NatE compl

23 N-terminal acetyl transferases (NATs), i.e., NatA, NatB, and NatC, which require as few as two specif

24 pment, which is independent of the essential NatA activity.

25 HYPK acts as a ribosome exchange factor for NatA.

26 We present evidence that human NatA catalyses N-terminal cysteine acetylation in vitro

27 molecular dynamics simulations of the human NatA and its S37P mutant highlight differences in region

28 al analyses of variants as part of the human NatA complex, as well as enzymatic analyses with and wit

29 unctionally important differences with human NatA and Candida albicans NatB, resolves key hNatB prote

30 f a neurodegenerative disease and implicates NatA-mediated Htt acetylation as a new potential therape

31 interacting protein K (HYPK), which inhibits NatA activity in vitro but enhances function in vivo.

32 at) in Arabidopsis (Arabidopsis thaliana) is NatA, which cotranslationally catalyzes acetylation of ~

33 NAA50 expression did not affect NTA of known NatA substrates and caused the accumulation of proteins

34 +)] phenotype is reversed in strains lacking NatA.

35 f three N-terminal acetyltransferases (NAT), NatA, NatB, and NatC, which contain Ard1p, Nat3p and Mak

36 To date, two eukaryotic NATs, NatA and NatE, have been structurally characterized, of

37 Indeed, loss of NatB function, but not NatA, increased plant sensitivity toward osmotic and hig

38 the Arg/N-end rule pathway in the absence of NatA, and showed that a number of Hsp90 clients are prev

39 plex and reveal evolutionary conservation of NatA biochemical properties in higher eukaryotes and unc

40 Strikingly, co-depletion of NatA, a heterodimeric NAT complex that physically intera

41 Accordingly, transgenic downregulation of NatA induces the drought stress response and results in

42 uncover specific and essential functions of NatA for development, biosynthetic pathways and stress r

43 Based on our data, we propose the name of NatA (N-acyltransferase A) in lieu of YiaC to reflect th

44 ealed a decreased acetylation of a subset of NatA and NatE substrates in Ogden syndrome cells, suppor

45 arding reduced Nt-acetylation of a subset of NatA/NatE-type substrates as one etiology for Ogden synd

46 ene encoding Naa10, the catalytic subunit of NatA, the major human NAT involved in the co-translation

47 Here we characterize the plant NatA complex and reveal evolutionary conservation of Nat

48 AA50 is catalytically inactive and positions NatA at the ribosome tunnel exit.

49 HYPK, hyper-tight ribosome binding prevents NatA from accessing additional ribosomes following each

50 Despite this change in phenotype, [PSI(+)] NatA mutants continue to propagate heritable Sup35([PSI+

51 Rather, NatA null strains are specifically impaired in establish

52 Purified recombinant NatA and Naa50 do not affect each other's NAT activity i

53 t caused by Naa50 depletion, indicating that NatA and Naa50 play antagonistic roles in cohesion.

54 The NatA effect cannot be explained by the modification of k

55 Sir3 and, perhaps, also Orc1 are the NatA substrates whose lack of acetylation in ard1 and na

56 Thus, we propose that NTA by the NatA complex acts as a cellular surveillance mechanism d

57 otein Sir3 and of Orc1 are acetylated by the NatA Nalpha-acetyltransferase.

58 equired for N-terminal acetylation, i.e. the NatA, NatB, and NatC substrates, were evaluated by consi

59 N-terminus is an in vitro substrate for the NatA N-terminal acetyltransferase and show that N-termin

60 eleases the inhibitory interactions from the NatA regulatory protein huntingtin yeast two-hybrid prot

61 impaired in naa10Delta cells, which lack the NatA N(alpha)-terminal acetylase (Nt-acetylase) and ther

62 iates with the Nat1 and Ard1 subunits of the NatA acetyltransferase.

63 ive site of ssNAT represents a hybrid of the NatA and NatE active sites, and we highlight features of

64 The auxiliary and regulatory subunits of the NatA complex are NAA15 and Huntington-interacting protei

65 l as Naa50 (NatE), another interactor of the NatA complex.

66 ld be overridden not only by ablation of the NatA Nt-acetylase but also by overexpression of the Arg/

67 h a small fraction of San interacts with the NatA complex, San appears to mediate cohesion independen

68 minal-acetylated, and 24 of these (80%) were NatA substrates, unacetylated in solely the ard1-Delta m

69 ve been structurally characterized, of which NatA will acetylate the alpha-amino group of a number of

70 n of the in vivo protein substrates of yeast NatA and NatB has been performed by N-terminomics.