ライフサイエンスコーパス: Nef protein

1 , an effect that is antagonized by the viral Nef protein.

2 IV-1(NL4-3) also created a dominant-negative Nef protein.

3 also generated in vitro using DC pulsed with Nef protein.

4 sine-based activation motif within the viral Nef protein.

5 ctivation of the c-kit promoter by the HIV-1 Nef protein.

6 T) from the cytoplasmic tail of CD4 binds to Nef protein.

7 ions which contained only 27-kDa full-length Nef protein.

8 SER5 from domestic cats and protected by its Nef protein.

9 rus-1 (HIV-1), which is antagonized by HIV-1 Nef protein.

10 ast in part, to be attributable to the HIV-2 Nef protein.

11 the SMR domain ((66)VGFPV(70)) of the HIV-1 Nef protein.

12 infectivity but is antagonized by the viral Nef protein.

13 booster immunizations with SIV gp140 and SIV Nef protein.

14 aining about 82% of the activity of the full Nef protein.

15 Tat, and Nef RNAs but do not express Tat and Nef proteins.

16 ength (32- to 36-kDa) or truncated (<32 kDa) Nef proteins.

17 imian or human immunodeficiency virus type 1 Nef proteins.

18 cells is indeed differentially modulated by Nef proteins.

19 scripts frequently encoded HIV-1 Gag-p17 and Nef proteins.

20 ally competent and produce the HIV-1 Gag and Nef proteins.

21 topes derived from virion-associated Gag and Nef proteins.

22 and -SIV nef recombinants and Tat, Env, and Nef proteins.

23 were predominantly focused on viral Gag and Nef proteins.

24 directed against epitopes within the Gag and Nef proteins.

25 t and Rev) and accessory (Vif, Vpr, Vpu, and Nef) proteins.

26 It has been presumed that Nef proteins accelerate endocytosis of CD4 by linking th

27 HIV-1 group M subtypes, we demonstrate that Nef proteins across all lentiviral lineages downregulate

28 We analyzed the anti-SERINC5 activity of Nef proteins across primate lentiviruses and examined wh

29 s unknown whether this property is common to Nef proteins across primate lentiviruses and how antivir

30 with the human immunodeficiency virus type I Nef protein activates Hck tyrosine kinase and biological

31 The Nef protein alters T cell receptor (TCR) signaling in T

32 st from the cytosol, to be replaced by novel NEF proteins, among which the Hsp110 family stands out.

33 were exposed to baculovirus-expressed HIV-1 Nef protein, an HIV-1-based vector expressing Nef, splee

34 We show here that negative factor (Nef) protein, an immunosuppressive human immunodeficienc

35 shed by mutations in the central part of the Nef protein and in particular by those known to disrupt

36 fectivity requires the presence of the viral Nef protein and the cellular protein cyclophilin A (CyPA

37 By using chimeric Nef proteins and site-directed mutagenesis, the amino ac

38 ressure to restore expression of a truncated Nef protein, and this reversion was linked to increased

39 SIV in chimpanzee (SIVcpz), and that SIVcpz Nef protein antagonizes chimpanzee tetherin.

40 Thus, specific sequences in the Nef protein appear to be necessary for Nef protein-induc

41 opithecini tetherin and demonstrate that all Nef proteins are capable of antagonizing ancestral Cerco

42 d human immunodeficiency virus (SIV and HIV) Nef proteins are important for virulence.

43 human immunodeficiency virus type 1 (HIV-1) Nef proteins are related regulatory proteins that share

44 Using the HIV-1 nef protein as a model tumor Ag, we found that in mice i

45 The role of the accessory viral Nef protein as a multifunctional manipulator of the host

46 ce: These data indicate that using the whole Nef protein as a vaccine immunogen likely allows immunod

47 c CTL responses generated to exogenous HIV-1 Nef protein as well as intracellularly expressed nef tra

48 s 174 and 175 in the full-length recombinant Nef protein background completely abrogated binding of c

49 I MHC nearly as effectively as the wild-type Nef protein, but was only about 60% as effective for CD4

50 ion of cell-surface CD4 induced by the viral Nef protein can fully reverse this inhibition and is, th

51 The HIV-1 Nef protein can impair ATP cassette binding transporter

52 cell cycle, we investigated whether the SIV Nef protein can modulate cell proliferation and apoptosi

53 Functionally, Nef proteins containing leucine motifs down-regulated CD

54 and 75 kDa, which do not interact with other Nef proteins, correlated with the decreased virion infec

55 ation of NefF12 suggested that the wild-type Nef protein could bind to assembly intermediates in late

56 This study suggests that extracellular Nef protein could contribute to the decline of CD4 count

57 ncy of disrupted nef open reading frames and Nef proteins defective for CD4 downregulation.

58 Analysis of a large number of mutant Nef proteins demonstrated that the effects of Nef on CD4

59 Here, we show that Nef proteins derived from many primary isolates of HIV-1

60 Most of the Nef proteins derived from three of four individuals with

61 Though lentiviral Nef proteins differed markedly in their absolute MHC-A a

62 Here, we analyzed the effect of Nef proteins differing in their T cell receptor (TCR)-CD

63 A fluorescently tagged motif peptide and Nef protein displayed physical binding to CXCR4-transfec

64 SIV and HIV Nef proteins disrupt T-cell receptor machinery by down-m

65 iciency virus (HIV) immune response, the HIV Nef protein disrupts major histocompatibility complex cl

66 The HIV Nef protein down-regulates the cell surface expression o

67 HIV-1 and SIV Nef proteins downregulate cell surface CD4 and MHC class

68 tion existed between the extent to which the Nef proteins downregulated cell surface MHC-I and CD4 an

69 Since the viral Nef protein downregulates MHC-I and provides infected ce

70 The HIV Nef protein downregulates the cell-surface expression of

71 CD4 downregulation induced by the Nef protein encoded by HIV-2 is shown to require a CD4 t

72 Although the Nef proteins encoded by human immunodeficiency virus typ

73 The Nef protein enhances human immunodeficiency virus type 1

74 The Nef protein enhances human immunodeficiency virus type 1

75 human immunodeficiency virus type 1 (HIV-1) Nef protein enhances viral infectivity by an unknown mec

76 The HIV-1 Nef protein enhances viral pathogenesis through multiple

77 mote kinase activity and show that the HIV-1 Nef protein evolved distinct mechanisms to activate Src

78 Human immunodeficiency virus type 1 (HIV-1) Nef protein exerts several effects, both on infected cel

79 ination assay showed that both HIV-1 and SIV Nef proteins expressed in Jurkat T cells and 293T cells

80 ein degradation; how the viral Vpu, Env, and Nef proteins facilitate internalization and degradation

81 Expression of the viral Nef protein from an adenovirus vector suggests that Nef

82 The Nef protein from human or simian immunodeficiency virus

83 ssue of Cell, Schindler et al. show that the Nef protein from nonpathogenic strains of simian immunod

84 A conserved feature of Nef proteins from different lentiviral lineages is the a

85 Here we show that Nef proteins from diverse groups of primate lentiviruses

86 Nef proteins from HIV type 1 (HIV-1), HIV-2, and SIV con

87 actor Vav as the specific binding partner of Nef proteins from HIV-1 and SIV.

88 Inspection of diverse Nef proteins from HIV-1, HIV-2, and simian immunodeficie

89 Nef proteins from human immunodeficiency virus type 1 (H

90 Nef proteins from human immunodeficiency virus type 1 is

91 site-specific mutagenesis to generate mutant Nef proteins fused to bacterial glutathione S-transferas

92 on of the human immunodeficiency virus (HIV) Nef protein has been linked to both decreased cell surfa

93 e, a highly conserved activity of lentiviral Nef proteins has dual effects and imposes both fitness c

94 The lentiviral Nef protein helps viruses evade innate and adaptive immu

95 Finally, viruses expressing a full-length Nef protein in conjunction with the changes in the TM ha

96 by Nef has been obtaining good expression of Nef protein in T cells.

97 V-1 peptides spanning the Gag, Pol, Env, and Nef proteins in addition to the baculovirus-derived p24

98 HIV and simian immunodeficiency virus (SIV) Nef proteins in the context of recent structural advance

99 Many functions have been ascribed to the Nef protein, including the down-regulation of cell surfa

100 ions via different surfaces in SIV and HIV-1 Nef proteins indicates that these interactions have crit

101 human immunodeficiency virus type 1 (HIV-1) Nef proteins induce the endocytosis of CD4 and class I M

102 tion of human macrophages with exogenous HIV Nef protein induced phosphorylation of Akt and GSK-3beta

103 n the Nef protein appear to be necessary for Nef protein-induced apoptosis as well as for physical in

104 HIV Nef protein inhibits ATP-binding cassette transporter (A

105 of evidence support the hypothesis that the Nef protein interacts directly with the cellular protein

106 The Nef protein is an important HIV virulence factor that pr

107 The HIV Nef protein is an important pathogenic factor that modul

108 human immunodeficiency virus type 1 (HIV-1) Nef protein is an important virulence factor.

109 The Nef protein is expressed early in infection and is neces

110 The HIV-1 Nef protein is important for pathogenesis, enhances vira

111 imal viral infectivity, the possibility that Nef protein is included in the virion was investigated.

112 HIV-1 particles have been demonstrated, the Nef protein is incorporated into HIV-1 particles.

113 In addition, a full-length Nef protein is necessary for optimum virus replication i

114 human immunodeficiency virus type 1 (HIV-1) Nef protein is required for efficient virus replication

115 In this study, HIV Nef protein is shown to associate with a serine-threonin

116 In the present study, extracellular Nef protein is shown to induce interleukin (IL)-10 mRNA

117 n of the human immunodeficiency virus type 1 Nef protein is the downregulation of CD4 from the surfac

118 The HIV Nef protein is thought to promote HIV immune evasion by

119 The Nef protein is unique to primate lentiviruses and is clo

120 CD4 left at the surface of cells expressing Nef proteins isolated from ECs are sufficient to allow E

121 Cells treated with Nef/protein kinase inhibitor complexes were protected fr

122 vitro binding studies using recombinant SIV Nef proteins lacking CAIDs and recombinant CD3-zeta cyto

123 ncomitant genetic diversity, the majority of Nef proteins maintained robust abilities to down-regulat

124 ormational change in the myristoylated HIV-1 Nef protein (myrNef): at high lipid packing density, myr

125 from Gag (Gag(71-79) GY9), and one from the Nef protein (Nef(159-167) YY9).

126 The atypical Nef protein (NefF12) from human immunodeficiency virus t

127 s revealed that while neither the mutant SIV Nef protein nor 8-zeta colocalized with AP-2 when expres

128 The Nef protein of HIV-1 and the entirely unrelated glycosyl

129 The Nef protein of HIV-1 downregulates the cell surface co-r

130 The Nef protein of HIV-1 facilitates viral replication and d

131 The multifunctional Nef protein of HIV-1 is important for the progression to

132 After two decades of research the Nef protein of human immunodeficiency virus (HIV) remain

133 uine infectious anemia virus (EIAV), and the Nef protein of human immunodeficiency virus type 1 (HIV-

134 The Nef protein of human immunodeficiency virus type 1 (HIV-

135 The Nef protein of human immunodeficiency virus type 1 downr

136 The Nef protein of human immunodeficiency virus-1 (HIV-1) ha

137 The Nef protein of human immunodeficiency virus-1 (HIV-1) is

138 A key function of the Nef protein of immunodeficiency viruses is the downregul

139 lved Vpu as a tetherin antagonist, while the Nef protein of less widespread HIV-1 group O strains acq

140 ment of amino acid residues 17 and 18 of the Nef protein of SIVmac239 with the corresponding amino ac

141 the corresponding amino acid residues of the Nef protein of SIVsmmPBj14 yielded a PBj-like virus that

142 ctivation motif (ITAM) that is unique to the Nef protein of the acutely pathogenic simian immunodefic

143 The Nef protein of the human immunodeficiency virus type 1 (

144 The Nef protein of the inferred common ancestor of group O v

145 The Nef protein of the primate lentiviruses human immunodefi

146 The Nef protein of the type 1 human immunodeficiency virus (

147 Here we show that the Nef proteins of certain HIV-1 group M isolates can acqui

148 Among the pleiotropic effects of Nef proteins of HIV and simian immunodeficiency virus (S

149 Here, we show that the Nef proteins of HIV-1 and other lentiviruses antagonize

150 The Nef proteins of human and simian immunodeficiency viruse

151 The Nef proteins of human immunodeficiency virus and simian

152 Nef proteins of primate lentiviruses promote viral repli

153 tagonists of Tetherin, including the Vpu and Nef proteins of primate lentiviruses.

154 in human tetherin prevents antagonism by the Nef proteins of SIVcpz and SIVgor, which represent the a

155 Furthermore, the Nef proteins of the highly pathogenic variants only slig

156 tional organization is conserved between the Nef proteins of the human and simian immunodeficiency vi

157 Overall, 6 (10%) of 60 persons had truncated Nef proteins; of these, 5 were among the 36 asymptomatic

158 hocytes (CTLs) due to the effects of the HIV Nef protein on antigen presentation by major histocompat

159 The effects of soluble Nef protein on CD4(+) T cells were examined.

160 This report describes a novel effect of the Nef protein on human T cells.

161 The human immunodeficiency virus type 1 Nef protein performs several functions potentially impor

162 The HIV-1 Nef protein plays a critical role in viral infectivity,

163 The Nef protein produced by the viruses HIV-1 and SIV drives

164 These Nef proteins promoted virus release and tetherin downmod

165 Here we examined HIV-1 Nef protein promotion of KSHV oncoprotein K1-induced ang

166 ade A, B, and C proteins was substantial for Nef proteins (ratio, 0.97 [95% confidence interval, 0.89

167 Lentiviral Nef proteins regulate the release of chemokines (MIP-1 a

168 We found that similar amounts of Nef protein resulted in a much more dramatic downmodulat

169 The analysis of three rare mutations in this Nef protein revealed that these effects could be separat

170 Amino acid sequence alignment with active Nef proteins revealed differences in regions not previou

171 HIV-1 Nef protein shares a significant homology with the immun

172 inding of a prolyl-rich segment from the HIV Nef protein slows unfolding by a factor of 3.

173 However, these modified Nef proteins still retained the ability to enhance viral

174 The HIV-1 Nef protein suppresses multiple immune surveillance mech

175 rus type 1 (HIV-1) and the majority of HIV-2 Nef proteins tested did not have such strong effects.

176 tyrosine substitution at position 17 in the Nef protein that is a major determinant of virulence but

177 -mediated anti-tetherin activity, acquired a Nef protein that is able to target a region adjacent to

178 We have designed a Nef protein that is readily expressed in T-cell lines an

179 s.IMPORTANCE Primate lentiviruses encode the Nef protein that plays an essential role in establishing

180 meric HIV-1 constructs expressing lentiviral Nef proteins that differ in their ability to down-modula

181 tyrosine motifs are not present in the HIV-1 Nef protein, the molecular basis for the presumed intera

182 ques with disease produced a novel truncated Nef protein, tNef.

183 st simian immunodeficiency viruses use their Nef protein to antagonize the host restriction factor te

184 r vesicles increased transfer of cytosol and Nef protein to endothelial monolayers in a Rac1-dependen

185 an immunodeficiency viruses (SIVs) use their Nef proteins to counteract the restriction factor tether

186 text of gene transfection, HIV infection, or Nef protein transduction.

187 human immunodeficiency virus type 1 (HIV-1) Nef protein upregulates the expression of the invariant

188 Thus, while SIV and HIV-1 Nef proteins use a similar mechanism to downregulate cla

189 nce between HIV-1 and HIV-2/SIV in which the Nef proteins utilize structurally distinct motifs for bi

190 Furthermore, we showed that the Nef protein was also required for the activation of pDCs

191 The HIV-1 Nef protein was analyzed for apoptotic structural motifs

192 found entirely in rafts while the wild-type Nef protein was distributed 10% in rafts and 90% in the

193 Since the LAT-Nef protein was found entirely in rafts while the wild-t

194 Greater than 90% of the LAT-Nef protein was found in the raft fraction.

195 The LAT-Nef protein was more efficient than its nonraft mutant c

196 n lipid rafts were not affected, this mutant Nef protein was poorly incorporated into viral particles

197 e constitutively expressing the native HIV-1 Nef protein was used to coimmunoprecipitate a stable Nef

198 ively high concentrations of exogenous HIV-1 Nef protein were able to induce apoptosis in MVECs.

199 ilar quantities of proteolytically processed Nef protein were detected in gradient fractions of HIV-1

200 ading frames that directed the expression of Nef protein were recovered from all of the individuals.

201 onses to clade E and B Gag, Pol-RT, Env, and Nef proteins were compared in 12 HLA-characterized, clad

202 While functional Nef proteins were demonstrated early in the course of in

203 The HIV-1 Rev, Tat, p17(Gag), and Nef proteins were directly phosphorylated by MAPK in vit

204 HIV-1 SF2 and 248 and SIVmac239 Nef proteins were found associated with the kinase.

205 HIV-1 NL4-3 and 233 Nef proteins were found weakly associated or not associa

206 We show that addition of the HIV-1 Nef protein, which is a high-affinity ligand for the Hck

207 mate lentiviruses (HIV-1, HIV-2, SIV) encode Nef proteins, which are important for viral replication

208 e physical binding of a fluorescently tagged Nef protein, while CCR5 antibodies were ineffective.

209 loops in the Nef molecule to co-localize the Nef protein with AP-2 adaptor complexes at the cell marg

210 eterologous SH4 domains resulted in chimeric Nef proteins with distinct steady state subcellular loca