ライフサイエンスコーパス: Northern blot

1 the Pneumocystis life cycle was analyzed by Northern blot.

2 8, and 24 hrs in septic and control rats by Northern blot.

3 icroRNA, however it could not be detected by Northern blot.

4 sitivity similar to small RNA sequencing and northern blots.

5 ; nine of these appeared to be stabilized on Northern blots.

6 mples from frontal and parietal cortex using Northern blots.

7 d cells using next-generation sequencing and northern blots.

8 pletion, confirmed experimentally using tRNA Northern blotting.

9 enic tiling microarray and were confirmed by northern blotting.

10 press Slc34a1 sense/antisense transcripts by northern blotting.

11 ing real-time quantitative PCR (RT-qPCR) and Northern blotting.

12 eal-time polymerase chain reaction (PCR) and northern blotting.

13 p in GCB-DLBCL cells were verified using RIP-Northern blotting.

14 was used to detect mRNA for the Lcn2 gene in Northern blotting.

15 nal for BPAG1 in the brain of dt-Alb mice by Northern blotting.

16 transcription polymerase chain reaction, and Northern blotting.

17 eq data and nine microRNAs were confirmed by northern blotting.

18 RNA sequencing, quantitative PCR (qPCR), and Northern blotting.

19 RNA samples and their subsequent analysis by Northern blotting.

20 in cancer tissue is confirmed by qRT-PCR and northern blot after oxidation/beta-elimination procedure

21 Northern blot analyses also demonstrated a positive role

22 ld restore GADD45beta expression in HepG2 in Northern blot analyses and quantitative real-time polyme

23 Northern blot analyses confirmed the associated molecula

24 Northern blot analyses demonstrate that CvMT-IV is down-

25 Southern blot and Northern blot analyses demonstrated hybridization of a r

26 Northern blot analyses demonstrated that liver expressed

27 Northern blot analyses detected full-length replicon RNA

28 rapid amplification of cDNA ends (RACE) and Northern blot analyses in several EBV-positive (EBV(+))

29 Transcriptional fusions and Northern blot analyses indicate that NanR represses the

30 Northern blot analyses indicate that SA suppresses GA-in

31 and polyamine transport and metabolism, and Northern blot analyses indicate that the AbcR sRNAs acce

32 In agreement, strand-specific RT-PCR and Northern blot analyses indicated that antisense transcri

33 Northern blot analyses of a subset of the neuronal genes

34 Northern blot analyses of infected-cell RNA showed that

35 Northern blot analyses of mitochondrial transcripts indi

36 Northern blot analyses provided evidence that three mRNA

37 Northern blot analyses revealed low expression levels of

38 Surprisingly, Northern blot analyses revealed that cidABC and lrgAB tr

39 Northern blot analyses revealed that the cidR mutation c

40 Northern blot analyses showed expression of EDIII antige

41 Furthermore, reverse transcriptase PCR and Northern blot analyses showed that cpb2 genes in all of

42 Microarray and Northern blot analyses showed that RR06 is specifically

43 Subsequent Northern blot analyses showed that the up-regulated tran

44 ure rodent microRNAs and quantitative RT-PCR/Northern blot analyses showed that Trx1 upregulates memb

45 onal analyses by transient transfections and Northern blot analyses suggest APBP-1 has the capacity t

46 Microarray and Northern blot analyses suggested that the expression of

47 In this study, we used microarray and Northern blot analyses to determine the S. pyogenes mRNA

48 oarray and/or quantitative real-time PCR and northern blot analyses, microRNA-218 (miR-218) was speci

49 oliferation and colony reduction assays, and Northern blot analyses, neither blocking of the binding

50 By Western and Northern blot analyses, NK1-FL and NK1-Tr were coexpress

51 ssed for immunocytochemistry and Western and Northern blot analyses, to determine stromal cell densit

52 Using 3'-rapid amplification cDNA end and Northern blot analyses, we identified the complete 3'-un

53 Several of these changes were confirmed by Northern blot analyses.

54 ion, Western blot, immunohistochemistry, and Northern blot analyses.

55 n as confirmed by transcriptional fusion and Northern blot analyses.

56 pairs identified in dsRNAs were confirmed by Northern blot analyses.

57 tive reverse transcription-PCR (qRT-PCR) and Northern blot analyses.

58 ression using quantitative real-time PCR and Northern blots analyses.

59 is study, we used proteomics and Western and Northern blotting analyses to demonstrate that alcohol d

60 roarray, reverse transcription (RT)-PCR, and Northern blotting analyses, we identified 11 virally enc

61 Northern-blot analyses performed on chloroplast transgen

62 Northern blot analysis and 3' rapid amplification of cDN

63 Northern blot analysis and 5' rapid amplification of cDN

64 Northern blot analysis and deep sequencing showed that i

65 Northern blot analysis and DNA binding studies of the pu

66 ally investigated six novel candidates using Northern blot analysis and found expression of three can

67 transcription-polymerase chain reaction and Northern blot analysis and of protein expression by West

68 Northern blot analysis and protein activity analysis dem

69 Using Northern blot analysis and transcriptional reporter gene

70 Northern blot analysis confirmed expression of all miRNA

71 Northern blot analysis confirmed that interferon-gamma h

72 Northern blot analysis confirmed the expression of many

73 Northern blot analysis confirmed upregulation of JIP-1.

74 Northern blot analysis demonstrated a close correlation

75 ption polymerase chain reaction (RT-PCR) and northern blot analysis demonstrated reduced expression o

76 Microarray and Northern blot analysis demonstrated that under low oxyge

77 In situ hybridization and Northern blot analysis demonstrated the presence of endo

78 Northern blot analysis detected the siRNAs products in v

79 Northern blot analysis detected the stable 2.0-kb LAT in

80 Northern blot analysis disclosed that teg49 and teg48 we

81 Northern blot analysis followed by sequencing of B3 prog

82 the count of cell number for proliferation, Northern blot analysis for gene expression (up to 10 day

83 adult cadaveric donors and were assessed by Northern blot analysis for growth and melanogenic respon

84 Northern blot analysis found that Wnt-1 strongly induced

85 findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon

86 TaqMan and Northern blot analysis indicate BTNL2 is predominantly e

87 Northern blot analysis indicated that Cgap1p regulates t

88 Northern blot analysis indicated that each TbAK isoform

89 Northern blot analysis indicates that human VIP1 is expr

90 Northern blot analysis involves the separation of RNA mo

91 Northern blot analysis of adult brain also showed a sele

92 ementary tissues in the developing limb, and Northern blot analysis of chick limb bud mRNA shows that

93 Northern blot analysis of EBOV-infected cells using GP-g

94 Northern blot analysis of PvCYP707As in bean showed a hi

95 Northern blot analysis of representative mRNAs confirmed

96 Northern blot analysis of RNA encapsidation in vivo of t

97 Northern blot analysis of RNA extracted from cells follo

98 , site-directed mutagenesis experiments, and Northern blot analysis of RNA produced by constructs ind

99 Northern blot analysis of total RNA from the O46E parent

100 IGFBP expression was evaluated by RT-PCR and Northern blot analysis of total RNA preparations.

101 Northern blot analysis revealed 1.5 kb hMCA transcripts

102 al samples by quantitative real-time PCR and Northern blot analysis revealed an inverse correlation b

103 Northern blot analysis revealed an inverse mRNA expressi

104 Northern blot analysis revealed that both of these genes

105 Northern blot analysis revealed that Bryo-1 treatment of

106 Northern blot analysis revealed that the expression of M

107 Northern blot analysis revealed that these transcripts w

108 Northern blot analysis revealed the presence of endogeno

109 Northern blot analysis revealed the sup70-65 tRNA(Gln)(C

110 Both promoterless dicistronic test and northern blot analysis show transcripts being derived fr

111 Northern blot analysis showed a 4.5-kb transcript that i

112 Northern blot analysis showed that croaker HIF-1alpha an

113 Northern blot analysis showed that miR160 is abundant in

114 Northern blot analysis showed that the mRNA level of ost

115 In the present study, northern blot analysis showed that, also in humans, NCBE

116 2 cluster of snoRNAs by RNase protection and northern blot analysis shows that the MBII-52 expressing

117 However, Northern blot analysis suggests that IGFBP-5 is the pred

118 atabases, RT-PCR, in situ hybridization, and Northern blot analysis that cells in the mouse retina ex

119 Microarray expression experiments and northern blot analysis using RNA obtained from human ski

120 Quantitative polymerase chain reaction and northern blot analysis verified that bound mRNA remained

121 To confirm this, Northern blot analysis was performed and showed that the

122 m (ArsRS) in this acid-inducible regulation, Northern blot analysis was performed with RNAs isolated

123 Northern blot analysis was used to measure hepatic mRNA

124 Northern blot analysis was used to verify ZBED4 mRNA exp

125 Using northern blot analysis we demonstrate that L1 splicing,

126 small RNA substrates were probed directly by Northern blot analysis with RNA from the RNase P TS muta

127 Northern blot analysis with sense RNA and strand-specifi

128 rythroid-specific expression was detected by Northern blot analysis, and maximal expression during th

129 ed by nuclear in vitro transcription run-on, northern blot analysis, and quantitative polymerase chai

130 Based on Northern blot analysis, exposure of PEL cells to hypoxia

131 d during chondrocyte maturation, as shown by Northern blot analysis, in situ hybridization, and real-

132 NAs (miRNAs), six of which were confirmed by Northern blot analysis, leaving four as provisional.

133 multiple molecular techniques, which include Northern blot analysis, real-time PCR, miRNA microarray,

134 Northern blot analysis, semiquantitative reverse transcr

135 features of IMPDH1 in the retina, including Northern blot analysis, serial analysis of gene expressi

136 ing an miRNA microarray screen, confirmed by Northern blot analysis, we defined a set of seven miRNAs

137 Using patch-clamp and Northern blot analysis, we explored the regulation of bT

138 xamining the expression pattern of miRNAs by Northern blot analysis, we found that Drosophila miRNAs

139 Using Northern blot analysis, we identified that miR-1 is spec

140 By Northern blot analysis, we identified the expected 7.5-k

141 ription run-on assays, promoter studies, and Northern blot analysis, while stability of the COL1A1 an

142 of these eight novel miRNAs was confirmed by Northern blot analysis.

143 A species (NP, F, HN, and L) was examined by Northern blot analysis.

144 e LPS binding protein CD14 were confirmed by Northern blot analysis.

145 miRNAs is validated by quantitative PCR and northern blot analysis.

146 Preferential gene induction was verified by Northern blot analysis.

147 te sRNAs, a third of which were validated by northern blot analysis.

148 ceptor for alpha-MSH (MC1-R), as assessed by Northern blot analysis.

149 by cDNA microarray analysis and confirmed by Northern blot analysis.

150 The mRNA expression pattern was examined by Northern blot analysis.

151 that miR-3676 and miR-4521 are tsRNAs using Northern blot analysis.

152 oop position and expression as determined by northern blot analysis.

153 in vivo primer extension experiments and by Northern blotting analysis.

154 rimer extension experiments, consistent with Northern blotting analysis.

155 obtained by degenerative RT-PCR and used for Northern blot and 5'-RACE analysis.

156 sence of nat-siRNAs is supported not only by Northern blot and genetic analyses, but also by the fact

157 ogs, and their metabolites was studied using Northern blot and patch clamp recording.

158 Northern blot and primer extension analyses identified t

159 Northern blot and qualitative PCR analyses demonstrated

160 Northern blot and quantitative real-time PCR analyses co

161 y expressed miRNAs were further confirmed by Northern blot and quantitative real-time polymerase chai

162 c-Myb overexpression was verified by Northern blot and quantitative reverse transcription-pol

163 Northern blot and quantitative reverse transcription-pol

164 Expression analysis using Northern Blot and quantitative RT-PCR confirmed 2.5- to

165 essed RNAs, out of which 6 were confirmed by northern blot and RACE.

166 Northern blot and real time PCR analyses revealed an ele

167 Northern blot and real-time PCR analyses suggested that

168 Using Northern blot and reporter replicon assays, we demonstra

169 Northern blot and reverse transcriptase PCR (RT-PCR) ana

170 Northern blot and reverse transcriptase-PCR studies indi

171 c tissues, including brain, as determined by Northern blot and reverse transcription-PCR analysis.

172 RNA could be identified in the cells by both Northern blot and RNase protection assay.

173 he presence of the variants was confirmed by Northern blot and RNase protection assays.

174 Northern blot and RT-PCR analyses confirmed an operon st

175 In silico as well as Northern blot and RT-PCR analyses indicated these genes

176 Northern blot and RT-qPCR experiments revealed specific

177 However, Northern blot and small RNA deep sequencing analyses ind

178 et of miRNAs with the results from small RNA Northern blot and that from miRNA quantitative RT-PCR.

179 Northern blot and transcription start site analyses reve

180 We confirmed this finding by Northern blot and Western blot analyses in cultured vasc

181 Both Northern blot and Western blot analyses were conducted o

182 hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments in wild-type

183 hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

184 hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

185 Genes with altered expression verified by Northern blot and/or quantitative PCR were considered ca

186 ysregulated miRNAs were further confirmed by Northern blot and/or real-time polymerase chain reaction

187 thelin 1 in culture media were measured with Northern blots and enzyme immunoassays, respectively.

188 Northern blots and enzyme-linked immunosorbent assay (EL

189 Ss) and validate novel RNA transcripts using Northern blots and luciferase promoter fusions.

190 While Northern blots and promoter:GUS expression patterns have

191 miRNA and their precursors was evaluated by Northern blots and real-time polymerase chain reaction,

192 Northern blots and RT-PCR analysis of total RNA revealed

193 by Livny et al., we tested 40 candidates by Northern blotting and confirmed the expression of nine n

194 Northern blotting and fluorescence microscopy indicate t

195 Using Northern blotting and quantitative reverse transcriptase

196 Northern blotting and reverse transcriptase PCR demonstr

197 both in vivo immunoprecipitation followed by Northern blotting and RNA degradation assays, we confirm

198 Northern blotting and studies with a series of chromosom

199 , (ii) extracted for total RNA isolation for Northern blotting and, (iii) analysed immunohistochemica

200 opargyl-puromycin incorporation experiments, Northern blot, and electron microscopy analyses demonstr

201 scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determin

202 with quantitative reverse transcription-PCR, Northern blot, and Western blot analyses, to characteriz

203 NA fluorescent in situ hybridization (FISH), Northern blots, and RNA sequencing-implicates MRP RNA in

204 and subgenomic RNA strands were detected by Northern blotting, and crystalline lattices of viral par

205 Pulse-chase, Northern blotting, and primer extension analyses in the

206 ghput deep RNA sequencing, quantitative PCR, Northern blotting, and rapid amplification of cDNA ends

207 els of siRNAs that are readily detectable by Northern blot, are loaded into RNA-induced silencing com

208 ncing, computational analysis, and sensitive northern blot assays, we show that constitutively expres

209 his shortcoming by demonstrating multiplexed northern blotting based on the mechanism of hybridizatio

210 We describe a new northern blot-based protocol (LED) for small RNA (approx

211 xes with MS-based protein identification and Northern blotting-based rRNA detection approaches identi

212 Northern blotting can be done in 2 d, but detection of a

213 RNase protection and northern blot confirmed microarray results.

214 Real-time RT-PCR and Northern blots confirmed that established core Mga regul

215 n, 195 small RNAs (sRNAs) were detected, and Northern blots confirmed that many sRNAs were induced by

216 Northern blots confirmed that the increase of codon-opti

217 ent quantitative real-time PCR (qRT-PCR) and Northern blotting demonstrated that the ebpABC mRNA tran

218 iab-8 RNA also encodes a miRNA, detected on Northern blots, derived from the hairpin complementary t

219 Expression of ncRNAs was studied by northern blotting during (i) the normal developmental cy

220 When using northern blotting during these studies, we discovered th

221 Northern blots enable detection of a target RNA of inter

222 Ribonuclease protection assays, Northern blotting, enzyme-linked immunosorbent assay, an

223 Northern blotting established that HCV dsRNA contained g

224 e sterile and very hard to generate, all the Northern blot experiments in these papers were performed

225 Northern blot experiments revealed that the amount of ms

226 tially, they were adopted from those used in Northern blot experiments, but an increasing number of p

227 Northern blotting experiments with rifampicin disclosed

228 d the detection of miRNA by hybridization in northern blotting experiments.

229 Northern blotting for individual miRNAs showed that the

230 Northern blotting found TCR mRNA expression predominantl

231 Northern blots from skeletal muscle total RNA showed sev

232 Northern blotting, gene-specific RT-PCR, RNA pull-down a

233 Northern blot hybridization analysis revealed that hlyBA

234 Northern blot hybridization analysis showed that the trx

235 re primarily 24 nt in length, as detected by Northern blot hybridization and by sequencing small RNAs

236 The microarray results were confirmed by Northern blot hybridization and quantitative reverse-tra

237 e detected during infection of Vero cells by Northern blot hybridization.

238 o various noxious agents were examined using Northern blot, in situ hybridization, and immunohistoche

239 Northern blotting, in situ hybridization, and reporter g

240 ains, and transcript analysis (by RT-PCR and northern blotting) indicated the effect was mainly trans

241 and quantitative, the main disadvantage of a northern blot is that it detects such RNA less sensitive

242 Currently, northern blot is the most widely used method for validat

243 This improved northern blotting is particularly useful to analyze prod

244 e alpha-subunit gene expression, assessed by Northern blots, it stimulated a luciferase reporter plas

245 This protocol describes an improved northern blot method that enhances detection of small RN

246 We established, using Northern blotting, nuclear run-on assays, and RT-PCR, th

247 Furthermore, Northern blots of PNPase or RhlB mutants showed that Rhl

248 High resolution northern blots of subject fibroblast RNA suggested incom

249 small interfering RNAs (siRNAs) followed by Northern blotting of GAPDH, expression of N(pro) had no

250 cE7 is detectable by both inverse RT-PCR and northern blotting of HPV16-transformed cells.

251 alyzed by reverse transcription (RT)-PCR and Northern blotting of lens total RNA, immunoblotting of l

252 levated ocular transcripts were confirmed by Northern blotting of RNA from Bbs4(-/-) and three additi

253 aches that complement RNA-seq, discover that northern blotting of small RNAs is biased against short

254 our novel miRNAs (miR-x1- miR-x4), either by northern blot or by Ribonuclease Protection Assay.

255 t always be consistent with the results from Northern blot or miRNA quantitative RT-PCR.

256 er to perform and more sensitive than either northern blotting or ribonuclease protection assays.

257 The northern blot, or RNA gel blot, is a widely used method

258 The HCR northern blot protocol takes approximately 1.5 days inde

259 Cells were analyzed by Western blotting, Northern blotting, pulse-chase analysis, and immunofluor

260 estern blot, nuclear in vitro transcription, Northern blot, qPCR, and promoter transactivation analys

261 ipt of the human liver-specific MIR122 using Northern blot, quantitative real-time polymerase chain r

262 Gene validation was done by Northern blot, quantitative reverse transcriptase-polyme

263 ethods used in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR)

264 y used to monitor gene expression, including Northern blotting, real-time polymerase chain reaction,

265 tion of cDNA ends assay, DNA sequencing, and Northern blot revealed 1.9- and 1.73-kb transcripts, whi

266 Northern blots revealed 1.2 kb and 3.2 kb mRNA species t

267 Northern blots revealed lower levels of Nmt2 expression

268 Northern blotting revealed a complex transcription patte

269 Northern blotting revealed a coordinated time-of-day-dep

270 While northern blotting revealed only these two mRNAs, both PC

271 nation of enzyme-linked immunosorbent assay, Northern blot, reverse transcriptase-polymerase chain re

272 Analyses of GFP fusions, Northern blotting, reverse transcription polymerase chai

273 Northern blotting, reverse transcription-PCR, and SMART-

274 tro translation, quantitative real-time PCR, Northern blot, ribonuclease protection assay, and monoci

275 Subsequent experiments using Northern blots, RT-PCR and real-time RT-PCR confirmed th

276 urified RNA can be used for analyses such as northern blotting, RT-PCR and microarrays.

277 examined TLR mRNA and protein expression by Northern blotting, RT-PCR, real-time RT-PCR, Western blo

278 Northern blotting showed that transcription of 2.4/2.1-k

279 t and extend our RNA sequencing results with northern blots, showing that tRNAs and Y RNAs in the non

280 uencing, and extended by a 5'-RACE assay and Northern blotting, showing that meiotic cells induce the

281 Quantitative RT-PCR and Northern blotting studies showed reduced or loss of expr

282 Northern blotting studies showed that the levels of the

283 nbiased expression data derived from a large Northern blot study.

284 Northern blots suggested that NF95 may be expressed at v

285 Northern blotting suggests that these anti-CRISPRs manip

286 show by rapid amplification of cDNA ends and Northern blotting that Drosophila CCDC56 protein and mtT

287 We showed by using microarray analysis and Northern blotting that several genes are negatively regu

288 ocial evolution, we used deep sequencing and Northern blots to isolate caste-associated miRNAs in the

289 Toure et al, 2004 used Northern blotting to show that the Y-linked genes Ssty1

290 Northern blotting using probes specific to hrcA, igr66 o

291 e found that the standard dose of UV used in northern blots was not the most efficient at immobilizin

292 Using Northern blot, we showed that the eNOS-derived 27-nt sho

293 By reporter gene fusion and Northern blotting, we found that sbcDC regulated capsule

294 genes derived from other techniques (such as Northern blots) were used as reference genes in RT-qPCR

295 Keratin expression was studied by Northern blot, Western blot, and confocal microscopy.

296 By using Northern blot, Western blot, and immunofluorescent stain

297 enomedullary chromaffin cells as detected by Northern blotting, Western blotting, and specific PAI-1

298 Northern blots with RNA from adult mice and in situ hybr

299 Northern blotting with a CTV derived probe for assessmen

300 Northern blotting with comX probes revealed two distinct