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1 2), and Pi using a single contrast agent and Overhauser-enhanced magnetic resonance imaging technique
2 r with electron spin-resonance lineshape and Overhauser dynamic nuclear polarization analysis to cons
4 ed into heterogeneous regions of the PEM and Overhauser dynamic nuclear polarization relaxometry, thi
6 by using a hyperpolarized (1)H-MRI, known as Overhauser enhanced MRI (OMRI), and an oxygen-sensitive
9 rface, is analyzed by the recently developed Overhauser dynamic nuclear polarization (ODNP) technique
12 n distances obtained from the rotating frame Overhauser effect spectroscopy (ROESY) NMR experiment, w
13 tion spectroscopy (TOCSY) and rotating frame Overhauser enhancement spectroscopy (ROESY) spectra of t
14 ward this end, we have used a rotating-frame Overhauser effect spectroscopy-type NMR pulse sequence w
17 vely transferring the spin polarization from Overhauser dynamic nuclear polarization (ODNP)-enhanced
18 hydration water using an emerging tool, (1)H Overhauser dynamic nuclear polarization (ODNP)-enhanced
19 eta turn that, from a [1H]-15N heteronuclear Overhauser effect experiment, appears to enjoy substanti
20 relaxation rates and [1H]-15N heteronuclear Overhauser effects of the backbone amides of the free an
23 verified by either (19)F-(1)H heteronuclear Overhauser spectroscopy (HOESY) or X-ray crystallography
25 to each other as demonstrated by interligand Overhauser effects between ubiquinone-1 and DBMIB or 2-n
41 me b5 were quantified using {1H}-15N nuclear Overhauser effect (nOe) measurements, which characterize
44 mics are primarily conducted with 1D nuclear Overhauser enhancement spectroscopy (NOESY) presat for w
45 nd angular restraints based on 1H-1H nuclear Overhauser effects (NOEs), hydrogen-bonding networks, 3J
46 ty complexes using (15)N R(1), R(2), nuclear Overhauser effect, and chemical-shift anisotropy dipolar
47 ithout an inhibitor is based on 2813 nuclear Overhauser effects (NOEs) and has an average RMSD to the
48 us their free components, whereas 2D nuclear Overhauser effect spectroscopy (NOESY) spectra suggest c
49 e tend to correlate together in a 2D nuclear Overhauser effect spectroscopy (NOESY) spectrum, thus op
51 d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 r
52 man RBCC protein, MID1) based on 670 nuclear Overhauser effect (NOE)-derived distance restraints, 12
53 d hydroxyl group was assigned with a nuclear Overhauser correlated spectroscopy experiment (1 alpha-H
59 ling constants, relaxation rates and nuclear Overhauser effect prediction applied to the three levels
60 Quantum mechanics calculations and nuclear Overhauser effect spectroscopy NMR studies suggest that
61 a, total correlated spectroscopy and nuclear Overhauser effect spectroscopy, show that the molecule e
64 Chemical shift indices (CSI) and nuclear Overhauser effects (NOE) with 600 MHz NMR and CD confirm
65 ings, isotropic chemical shifts, and nuclear Overhauser effects (NOEs)/rotational frame nuclear Overh
69 nuclear single quantum coherence and nuclear Overhauser enhancement spectroscopy spectra for the trik
73 based on 2778 unambiguously assigned nuclear Overhauser effect (NOE) connectivities, 297 ambiguous NO
75 of NMR chemical shifts and backbone nuclear Overhauser effect (NOE) connectivities showed that OspA[
81 p to 19,000 M(-1)), and is shown--by nuclear Overhauser effect spectroscopy--to adopt the threading g
83 ent alignment media, supplemented by nuclear Overhauser enhancement data and torsion angle restraints
84 hatic resonances in [Ca(2+)](4)-CaM (nuclear Overhauser effect) and increases the Ca(2+) affinity of
85 n this procedure, the time-consuming nuclear Overhauser enhancement (NOE)-based sequential assignment
86 s), intensities of NOESY crosspeaks [nuclear Overhauser effects (NOEs)], and residual dipolar couplin
88 was assigned through two-dimensional nuclear Overhauser effect spectroscopic analysis coupled with co
89 in combination with two-dimensional nuclear Overhauser effect spectroscopy (NOESY) results, demonstr
90 tion of (1)H NMR and two-dimensional Nuclear Overhauser Effect Spectroscopy (NOESY) which revealed fu
91 mined by transferred two-dimensional nuclear Overhauser effect spectroscopy (TRNOESY) measurements an
93 cular dichroism, and two-dimensional nuclear Overhauser enhancement spectra conclusively proves that
94 -relaxation rates in two-dimensional nuclear Overhauser enhancement spectroscopy NMR experiments show
96 paramagnetic probes and protein-DPC nuclear Overhauser effects (NOEs), we define portions of the gro
99 ntegration of simulations with exact nuclear Overhauser enhancements data allowed us to characterize
100 e techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( app
103 tored by two-dimensional (19)F-(19)F nuclear Overhauser effect, the distance between two phenylalanin
104 ) selective detection of (19)F-(19)F nuclear Overhauser effects in the Escherichia coli peptidyl-prol
106 dispersion experiments, and filtered nuclear Overhauser effects suggest that CCL27 does not adopt a d
108 ation parameters, and rotating-frame nuclear Overhauser effect spectroscopy (ROESY) crosspeaks that c
109 troscopy (NOESY), and rotating frame nuclear Overhauser effect spectroscopy (ROESY) data were recorde
110 red spectroscopy, and rotating-frame nuclear Overhauser effect spectroscopy and high-resolution elect
112 distinct structures are derived from nuclear Overhauser effect spectroscopic distance restraints coup
113 ing using structural restraints from nuclear Overhauser effect spectroscopy, and scalar and residual
116 straints (1074) were determined from nuclear Overhauser enhancements and main-chain torsion-angle con
117 ion of the cations as extracted from nuclear Overhauser experiments is in line with the preferred con
119 differences in homonuclear (1)H-(1)H nuclear Overhauser effects (NOEs) and heteronuclear (1)H-(15)N N
120 ntermolecular (1)H(19)F and (1)H(1)H nuclear Overhauser effects were used to explore interaction of s
121 ng (15)N-(13)C-labeled protein, (1)H nuclear Overhauser effects, and longitudinal relaxation data ide
122 these interactions through both (1)H nuclear Overhauser enhancement (NOE) and paramagnetic relaxation
123 ation rate (R(2)), and heteronuclear nuclear Overhauser effect (NOE) have been carried out at 11.7T a
124 axation rate (R2), and heteronuclear nuclear Overhauser effect (NOE)] measured at two temperatures (2
126 attice, spin-spin, and heteronuclear nuclear Overhauser effect relaxation data for backbone amide (15
127 13)C T(1), T(1rho) and heteronuclear nuclear Overhauser effects (NOEs) for sugar and base nuclei, as
128 ady-state {(1)H}-(15)N heteronuclear nuclear Overhauser effects indicate that the protein's core is r
132 ion NMR experiments of heteronuclear nuclear Overhauser enhancement (NOE), spin-lattice (R(1)), and s
133 The PBEs, in combination with HN-HN nuclear Overhauser effects (NOEs) and chemical shift index (CSI)
134 (15)N-T(1), (15)N-T(2), (15)N-{(1)H} nuclear Overhauser effect, reduced spectral density, and general
135 n cross-peaks were well dispersed in nuclear Overhauser effect and heteronuclear single quantum coher
136 eling and NMR spectroscopy including nuclear Overhauser effects and residual dipolar coupling of a sa
138 ANA to build a network of interchain nuclear Overhauser effect constraints that can be used to accura
139 on as evidenced by an intermolecular nuclear Overhauser effect (NOE) between each metallopeptide His
140 rane, as supported by intermolecular nuclear Overhauser effect cross-peaks between the peptide and sh
141 on the observation of intermolecular nuclear Overhauser effects (NOE) and their assignments, which ar
143 hemical shifts and 24 intermolecular nuclear Overhauser effects (NOEs) identify the 5'-ApG and 5'-GpT
144 cal shift mapping and intermolecular nuclear Overhauser effects (NOEs) indicate the presence of at le
146 zone derived from the intermolecular nuclear Overhauser effects is in agreement with recent biochemic
147 the talin rod and use intermolecular nuclear Overhauser effects to determine the structure of the com
148 itration calorimetry, intermolecular nuclear Overhauser effects, mutagenesis, and protection from par
150 aling on the basis of intermolecular nuclear Overhauser enhancement data and residual dipolar couplin
151 translational (i.e., intermolecular nuclear Overhauser enhancement, NOE, data) and orientational (i.
152 generated a number of intermolecular nuclear Overhauser enhancements (NOEs) and chemical shift pertur
153 perimentally resolved intermolecular nuclear Overhauser enhancements (NOEs) are extremely weak; most
154 produced a number of intermolecular nuclear Overhauser enhancements (NOEs) to residues in TMs 6 and
155 resonance assignments, interpreting nuclear Overhauser effect (NOE) spectroscopy (NOESY) spectra, an
156 itrogens, and (1)H-(1)H interresidue nuclear Overhauser effects (NOEs) for the two mutants with those
162 ential NMR assignments, intramonomer nuclear Overhauser effects, and circular dichroism spectra are c
166 he pattern of observed peptide-lipid nuclear Overhauser effects is consistent with a parallel orienta
167 and (1)H magic angle spinning (MAS) nuclear Overhauser effect spectroscopy (NOESY) techniques, we sh
170 has a high tolerance for misassigned nuclear Overhauser effect restraints, greatly simplifying NMR st
173 s interpretation of multidimensional nuclear Overhauser spectra for high-resolution structure determi
174 m-range alphaN(i,i+2) of each mutant nuclear Overhauser enhancements were observed in the urea-unfold
175 Similar chemical shifts and (15)N nuclear Overhauser effect (NOE) patterns of the peptide in compl
178 red by NMR experiments of (1)H-(15)N nuclear Overhauser effect, spin-lattice relaxation, and spin-spi
180 on data and steady-state (1)H- (15)N nuclear Overhauser effects were analyzed using model-free formal
181 elaxation times and the {(1)H}-(15)N nuclear Overhauser enhancement (nOe) of uniformly (15)N-enriched
183 acterized using isotope-edited (15)N nuclear Overhauser enhancement spectroscopy heteronuclear single
184 C) domain (as probed by {(1)H}-(15)N nuclear Overhauser enhancements) is progressively less ordered.
189 ns in solution from experimental NMR nuclear Overhauser effect data only and with minimal assignments
190 reflected in optical spectra and NMR nuclear Overhauser effect spectroscopy cross-peak and hyperfine
191 distance restraints derived from NMR nuclear Overhauser enhancement (NOE) data to predict protein str
192 A combination of FT-IR, (1)H NMR, nuclear Overhauser effect (NOESY), and diffusion-ordered (DOSY)
193 ton of one of the G.A base-pairs, no nuclear Overhauser enhancement cross-peaks between the cobalt li
196 these H3(+) resonance; and observed nuclear Overhauser effects consistent with Hoogsteen and Watson-
197 assigned through (a) measurement of nuclear Overhauser effect connectivities, (b) prediction of the
198 s method includes the acquisition of nuclear Overhauser effect spectroscopy one-dimensional and J-res
199 ucture as indicated by the number of nuclear Overhauser effects and is shown to play a critical role
200 assignments and detailed analysis of nuclear Overhauser effects permit the direct comparison of the f
204 ess, structure calculations based on nuclear Overhauser effect spectroscopic data combined with (15)N
209 ted peptide and by weak medium-range nuclear Overhauser effect contacts indicative of alpha-helical c
210 ty of both sequential and long-range nuclear Overhauser effects (NOEs) between backbone amide protons
212 lly inconsistent group of long range nuclear Overhauser effects suggest a close proximity of the heli
213 s fewer helix-related and long range nuclear Overhauser effects than does the d-Ser(B8) analog or nat
216 eteronuclear cross relaxation rates (nuclear Overhauser effect), suggesting that the 14-38 disulfide
218 ng range (|i - j| > or = 5 residues) nuclear Overhauser enhancement restraints were derived exclusive
220 ions lead to attenuation of selected nuclear Overhauser enhancements and accelerated amide proton exc
221 rt these assignments with sequential nuclear Overhauser effect (NOE) information obtained from a two-
223 riments (correlated chemical shifts, nuclear Overhauser effects, residual dipolar couplings) to predi
226 experiments with the through-space (nuclear Overhauser enhancement spectroscopy, NOESY) experiment.
228 old could be determined using sparse nuclear Overhauser enhancement (NOE) distance restraints involvi
229 een obtained using NMR spectrometry, nuclear Overhauser effects, and density functional theory to det
230 elaxation data, T1, and steady-state nuclear Overhauser effect (NOE) obtained at two different magnet
231 ), T(2), T(1)(rho), and steady-state nuclear Overhauser effects were measured at 500 and 600 MHz.
232 Distance bounds, calculated from the nuclear Overhauser effect (NOE) crosspeak intensities via a comp
233 distance restraints derived from the nuclear Overhauser effect (NOE) data were used to calculate the
236 different (1)H environments via the nuclear Overhauser effect (NOE) is included in the NMR pulse seq
237 istance restraints, analogous to the nuclear Overhauser effect (NOE) routinely used in solution NMR.
238 he resulting process is equal to the nuclear Overhauser effect (NOE) where typically continuous satur
241 on of conformational exchange to the nuclear Overhauser effect peak intensity, we applied inferential
242 nd guanine can be extracted from the nuclear Overhauser effect spectroscopy spectrum based on the clo
245 e was determined on the basis of the nuclear Overhauser effects (NOEs) and the hydrogen bond restrain
249 ods, using a model-based approach to nuclear Overhauser enhancement spectroscopy peak assignment.
250 al correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and rotating fra
251 R) experiments, and (5) NMR transfer nuclear Overhauser effect spectroscopy (NOESY) experiments that
252 Analysis by two-dimensional transfer nuclear Overhauser effect spectroscopy of the induced solution s
253 py experiments, inducing transferred nuclear Overhauser effect (trNOE) and saturation transfer differ
256 mined by two-dimensional transferred nuclear Overhauser effect (TRNOESY) measurements combined with m
257 mined by two-dimensional transferred nuclear Overhauser effect (TRNOESY) measurements combined with m
259 rmore, based on exchange-transferred nuclear Overhauser effect measurements, we established that MBM1
260 ed from a combination of transferred nuclear Overhauser effect NMR experiments and molecular dynamics
262 recovery method, and the transferred nuclear Overhauser effect spectroscopy was used to study the bin
266 tide was determined from transferred nuclear Overhauser effects (trnOe) experiments to determine inte
267 surfaces are studied by transferred nuclear Overhauser effects (trNOEs) and saturation transfer diff
272 ion times made it impractical to use nuclear Overhauser effect (NOE) measurements for assignment purp
274 ion of intermolecular contacts using nuclear Overhauser effect (NOE) experiments; (iv) structure dete
275 A structure calculated by using nuclear Overhauser effect and other NMR constraints reveals that
277 rmational ensemble obtained by using nuclear Overhauser effect data in structure calculations reveale
279 spholipid micelle interactions using nuclear Overhauser effect spectroscopy and showed that the micel
280 membrane proteins in nanodiscs using nuclear Overhauser enhancement spectroscopy (NOESY) spectroscopy
282 This is accomplished by utilizing nuclear Overhauser effect spectroscopy (NOESY) at subzero temper
284 l membrane proteins >15 kDa in size, Nuclear-Overhauser effect-derived distance restraints are diffic
285 e width of 2.1 G allowing for an increase of Overhauser enhancements and reduction in rf power deposi
288 le-temperature NMR studies and 2D rotational Overhauser effect spectroscopy NMR experiments have show
289 couplings, (15)N chemical shifts, rotational Overhauser effects, and residual dipolar couplings were
290 provided by the site-specific and selective Overhauser dynamic nuclear polarization of solvent molec
293 sample, the spin noise spectrum revealed the Overhauser field created by optically oriented nuclei an
295 l by up to 2 orders of magnitude through the Overhauser effect under ambient conditions at 0.35 tesla
297 continuous in situ hyperpolarization via the Overhauser mechanism, in combination with the excellent
300 led SOS-NMR for structural information using Overhauser effects and selective labeling and is validat