ライフサイエンスコーパス: P. mirabilis

1 P. mirabilis bacteriuria may lead to acute pyelonephriti

2 P. mirabilis HI4320 carrying the UreD-GFP fusion plasmid

3 P. mirabilis is capable of swarming, a form of multicell

4 P. mirabilis strain HI4320 encodes two putative nickel i

5 P. mirabilis(pBAC001), which expresses green fluorescent

6 By performing primary screening of 2088 P. mirabilis transposon mutants, we identified 502 mutan

7 , of 683 E. coli, 371 K. pneumoniae, and 232 P. mirabilis isolates tested, 13 (1.9%), 28 (7.6%), and

8 lows: A. baumannii (5%), K. pneumoniae (3%), P. mirabilis (3%), Enterobacter species (3%), Citrobacte

9 On agar, a P. mirabilis colony grows outward in a bull's-eye patter

10 A cosmid clone, isolated from a P. mirabilis genomic library by hybridization with the 3

11 In a P. mirabilis speA mutant with greatly reduced levels of

12 were not observed during coinfection with a P. mirabilis urease mutant.

13 ed significant anti-biofilm activity against P. mirabilis, reducing 24-hour established biofilms by 5

14 Screening for the presence of ICEPm1 among P. mirabilis colonizing isolates showed that ICEPm1 is m

15 To identify interactions among P. mirabilis accessory proteins, in vitro immunoprecipit

16 this study, microarrays were used to analyze P. mirabilis gene expression in vivo from experimentally

17 Fluorescent E. coli and P. mirabilis bacteria were observed by fluorescence micr

18 In particular, for both the E. coli and P. mirabilis orthologs, Lrp responsiveness to methionine

19 E. coli and P. mirabilis remained susceptible to most of the drugs t

20 y urea in both Escherichia coli DH5alpha and P. mirabilis HI4320.

21 onal start sites for the plasmid-encoded and P. mirabilis divergent promoters were similar in an Esch

22 ranscription at both the plasmid-encoded and P. mirabilis promoters.

23 inding sites in both the plasmid-encoded and P. mirabilis ureRp-ureDp intergenic regions.

24 ility against S. aureus, S. epidermidis, and P. mirabilis without any cytotoxic impact on mammalian c

25 tracellular survival of L. monocytogenes and P. mirabilis was assayed.

26 our institution, E. coli, K. pneumoniae, and P. mirabilis harbor plasmid-mediated AmpC enzymes.

27 s), Enterococcus faecalis (two strains), and P. mirabilis, E. coli (two strains), with statistically

28 r ureolytic bacteria such as P. stuartii and P. mirabilis, even in the presence of a non-ureolytic ba

29 imited zinc present in the urinary tract and P. mirabilis must scavenge this ion to colonize and pers

30 reased internalization of S. typhimurium and P. mirabilis by both HT-29 and Caco-2 enterocytes and wi

31 ined the epidemiology of catheter-associated P. mirabilis infections by use of pulsed-field gel elect

32 viously determined that interactions between P. mirabilis and other uropathogens can enhance P. mirab

33 Our results establish a relationship between P. mirabilis flagellum density and cell motility in visc

34 While there are similarities between P. mirabilis MR/P and E. coli P fimbriae, there are more

35 pectedly and unlike other fliL mutants, both P. mirabilis and E. coli DeltafliL cells swarm (Swr(+)).

36 ing the formation of crystalline biofilms by P. mirabilis.

37 /P) fimbriae, a surface antigen expressed by P. mirabilis during experimental urinary tract infection

38 nsight into crystalline biofilm formation by P. mirabilis, including the link between biofilm formati

39 rotection against urinary tract infection by P. mirabilis (P < 0.002).

40 ce from ascending urinary tract infection by P. mirabilis (P < 0.05).

41 obial catheter colonization, particularly by P. mirabilis and other urease-positive bacteria.

42 A recombinant plasmid containing cloned P. mirabilis hns was able to complement and restore repr

43 used by Enterobacterales, including E. coli, P. mirabilis, and Klebsiella pneumoniae, as well as gram

44 entification of 37 consistently out-competed P. mirabilis transposon mutants, 25 of which were out-co

45 ate metabolites, seven reproducibly decrease P. mirabilis urease activity.

46 5) transconjugants/donor to ICEPm1-deficient P. mirabilis using plate mating assays with clinical iso

47 ability to sense a surface: e.g., DeltafliL P. mirabilis cells swarm precociously over surfaces with

48 form between swarming colonies of different P. mirabilis strains but not between colonies of a singl

49 ic of urease-mediated urea hydrolysis during P. mirabilis infection.

50 ormed significantly more biofilm than either P. mirabilis HI4320 (P = 0.03) or MR/P OFF (P = 0.05).

51 mirabilis and other uropathogens can enhance P. mirabilis urease activity, resulting in greater disea

52 by this organism, was sufficient to enhance P. mirabilis urease activity and increase disease severi

53 Other uropathogens also enhanced P. mirabilis urease activity in vitro, including recent

54 Excluding P. mirabilis, MEV Etest MEV demonstrated 95.8% EA, 99.3%

55 ant) isolates of Enterobacterales (excluding P. mirabilis) and P. aeruginosa demonstrated an unaccept

56 othesized that nickel import is critical for P. mirabilis urease activity and pathogenesis during inf

57 In order for P. mirabilis to swarm, it first needs to detect a surfac

58 d key metabolic pathways as requirements for P. mirabilis infection of the urinary tract.

59 ine metabolism as an adaptation strategy for P. mirabilis and contributes to better understand the ec

60 rtance of siderophore production in vivo for P. mirabilis.

61 Modeling CaiT from P. mirabilis in the outward-open and closed states on th

62 How P. mirabilis senses a surface is not fully understood; h

63 Recent work has elucidated how P. mirabilis causes all of these disease states.

64 After 7 days, however, P. mirabilis HI4320 formed a 65-mum-thick biofilm, while

65 monia availability due to urease activity in P. mirabilis did not drive this gene expression.

66 Inactivation of aipA in P. mirabilis strains significantly (P < 0.01) reduced th

67 Although in P. mirabilis HI4320, ICEPm1 is annotated as integrated i

68 ty and antigenicity of an autotransporter in P. mirabilis and its use in vaccine development.

69 characterizing trimeric autotransporters in P. mirabilis as afimbrial surface adhesins and autoagglu

70 unclear how energetically costly changes in P. mirabilis cell morphology translate into an advantage

71 ve putrescine importer, was characterized in P. mirabilis.

72 ar details of self-nonself discrimination in P. mirabilis.

73 oxetine and thioridazine inhibited efflux in P. mirabilis, and molecular modelling predicted both dru

74 uoxetine and thioridazine) to act as EPIs in P. mirabilis, and control crystalline biofilm formation.

75 insertion was mapped to a speA homologue in P. mirabilis.

76 However, in P. mirabilis HI4320, transcription of ureR initiated pre

77 ellar basal body protein FliL is involved in P. mirabilis surface sensing.

78 Swimming and swarming motilies in P. mirabilis were also significantly reduced by both EPI

79 ivity and repression of the urease operon in P. mirabilis.

80 at the 3' end of the mrp fimbrial operon in P. mirabilis.

81 eus mirabilis; ectopic expression of papX in P. mirabilis reduces motility.

82 as an immunogenic outer membrane protein in P. mirabilis.

83 the first report of carbapenem resistance in P. mirabilis caused by the acquisition of bla(KPC).

84 This chromosome integration system in P. mirabilis provides an important tool for animal and b

85 veral related Phytophthora species including P. mirabilis, P. ipomoeae, and possibly P. phaseoli.

86 study shows that Premi effectively inhibits P. mirabilis biofilms and could be a promising antimicro

87 y tract, a ureR mutation was introduced into P. mirabilis HI4320 by homologous recombination.

88 Tn7 site-specific transposition pathway into P. mirabilis by transformation, followed by selection of

89 In the lab, P. mirabilis cells become long and multinucleate and inc

90 In liquid, P. mirabilis cells are 1.5- to 2.0-mum swimmer cells wit

91 hat common urinary tract colonizers modulate P. mirabilis urease activity via secreted small molecule

92 rovidencia stuartii, and Morganella morganii P. mirabilis infections are particularly challenging due

93 ded that MR/P fimbriae are expressed by most P. mirabilis cells infecting the urinary tract, dictate

94 a contribute significantly to the ability of P. mirabilis to colonize the urinary tract and cause acu

95 us, ids genes are involved in the ability of P. mirabilis to distinguish self from nonself.

96 Microarray analysis of P. mirabilis HI4320 cultured under iron limitation ident

97 ity, the AT also promoted autoaggregation of P. mirabilis and this function was independent of its pr

98 The crystalline biofilms of P. mirabilis can cause serious complications for patient

99 the flagellar operon, in vegetative cells of P. mirabilis and found that increased flagellum density

100 challenged transurethrally with 10(7) CFU of P. mirabilis BA6163 (wild type) (n = 16), WPM111 (hpmA m

101 rom infection (mean log(10) number of CFU of P. mirabilis Nal(r) HI4320 per milliliter or gram in vac

102 he initial epidemiologic characterization of P. mirabilis isolates.

103 (encoding the entire urease gene cluster of P. mirabilis) was equivalent in both the H-NS(-) backgro

104 may be a significant virulence component of P. mirabilis in urinary tract infections.

105 eye colony often associated with cultures of P. mirabilis.

106 n identified as important for development of P. mirabilis crystalline biofilms, highlighting the pote

107 e often invasive, and, with the exception of P. mirabilis, are multiclonal.

108 and Enterobacterales, with the exception of P. mirabilis, using CLSI/FDA breakpoints.

109 nces swarming-associated colony expansion of P. mirabilis under anaerobic conditions on a solid surfa

110 To investigate in vivo expression of P. mirabilis urease, the gene encoding green fluorescent

111 pproach to investigate in vivo expression of P. mirabilis virulence genes in experimental urinary tra

112 Ambient-temperature fimbriae of P. mirabilis may represent a novel type of fimbriae of e

113 patients revealed that a single genotype of P. mirabilis can persist in the urinary tract despite ma

114 Transurethral inoculation of P. mirabilis(pBAC001) resulted in ascending urinary trac

115 mrpI null mutants from a clinical isolate of P. mirabilis, HI4320.

116 ity against 4 out of 30 clinical isolates of P. mirabilis tested and is stable when exposed to pH val

117 Examination of a set of 55 isolates of P. mirabilis, each from a different clinical or environm

118 Immunogold electron microscopy of P. mirabilis HI4320 revealed that MrpH was located at th

119 ated autoagglutination, and a taaP mutant of P. mirabilis showed significantly (P < 0.05) more reduce

120 H5alpha and an isogenic mrpH::aphA mutant of P. mirabilis were unable to produce normal MR/P fimbriae

121 otype on an otherwise nonhemolytic mutant of P. mirabilis.

122 icantly reduced in an isogenic pta mutant of P. mirabilis.

123 ucted and characterized DeltafliL mutants of P. mirabilis and Escherichia coli.

124 I to evaluate the colonization of mutants of P. mirabilis HI4320 that were generated by signature-tag

125 The number of P. mirabilis cells adhering to bladder tissue did not ap

126 ociated and important for the persistence of P. mirabilis in the host, it was selected as a vaccine c

127 y contributes to the pathogenic potential of P. mirabilis in the urinary tract.

128 microscopy, we demonstrated the presence of P. mirabilis within the urease-induced stone matrix.

129 inhibitors in the treatment or prevention of P. mirabilis crystalline biofilms.

130 so found to significantly reduce the rate of P. mirabilis crystalline biofilm formation on catheters,

131 port a role of MrpJ as a global regulator of P. mirabilis virulence.

132 re we report the complete genome sequence of P. mirabilis HI4320, a representative strain cultured in

133 with the newly completed genome sequence of P. mirabilis HI4320, was used to identify surface-expose

134 Strains of P. mirabilis with mutations in three of the correspondin

135 ethods for identifying individual strains of P. mirabilis.

136 ng deposits in bladder and kidney tissues of P. mirabilis-infected mice.

137 also contributes to the uropathogenicity of P. mirabilis.

138 tion of urease by urea, and for virulence of P. mirabilis in the urinary tract.

139 resent work reveals that M. morganii acts on P. mirabilis in a contact-independent manner to decrease

140 eted into the culture medium by the original P. mirabilis flgN mutant demonstrated that export of Flg

141 Overall, P. mirabilis exhibits an extraordinary lifestyle, and fu

142 odes a 135-amino acid residue protein, PMTR (P. mirabilis transcription regulator), a new member of t

143 One of seven positive P. mirabilis isolates was in group II, with the remainde

144 imicrobial agent for treating drug-resistant P. mirabilis infection.

145 Five of seven P. mirabilis isolates were from blood cultures.

146 the CTX-M-positive isolates showed that six P. mirabilis isolates were clonal and that there were se

147 We conclude that P. mirabilis and P. stuartii coinfection promotes urolit

148 These results support the hypothesis that P. mirabilis ascertains its location in the environment

149 Further analysis revealed that P. mirabilis DeltafliL cells also exhibit an alteration

150 In this study, we show that P. mirabilis CaUTI isolates initiate swarming in respons

151 -feeding and biochemical analysis shows that P. mirabilis is unable to utilize or produce yersiniabac

152 annot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor car

153 In addition, the P. mirabilis HI4320 genome possesses four tandem copies

154 sbA (regulator of swarming behavior) and the P. mirabilis homologs to RcsB and RcsC.

155 Sequencing of the P. mirabilis genome revealed 14 additional paralogues of

156 These data underscore the importance of the P. mirabilis IgA-degrading metalloprotease in UTI.

157 und to be localized to a 5.4-kb locus on the P. mirabilis genome encoding RsbA (regulator of swarming

158 The results presented here suggest that the P. mirabilis and plasmid-encoded urease gene clusters ut

159 In this report, we demonstrate that the P. mirabilis urease gene cluster contains similar diverg

160 We show that the P. mirabilis, Vibrio harveyi, and E. coli Crl homologs f

161 Thus, P. mirabilis appears to use a related mechanism to inhib

162 and TaaP individually offered advantages to P. mirabilis in a murine model.

163 more prevalent in urine isolates compared to P. mirabilis strains isolated from other body sites (P<0

164 The majority of wild-type P. mirabilis cells in transurethrally infected mice prod

165 Wild-type P. mirabilis was usually found colonizing intact uroepit

166 In wild-type P. mirabilis, increased expression of the flhDC operon o

167 ncatheterized mice to infection by wild-type P. mirabilis.

168 bladder colonization factor of uropathogenic P. mirabilis and also suggested that the ability to swit

169 This report suggests that in vivo, P. mirabilis UreD may be important for recruitment of Ur

170 When P. mirabilis encounters a highly viscous environment, e.

171 When P. mirabilis encounters a solid surface, where flagellar

172 on and motility, a result also observed when P. mirabilis fliL+ was expressed in Escherichia coli.

173 the consolidation phase is a state in which P. mirabilis prepares for the next wave of swarming.

174 ull's-eye colonies typically associated with P. mirabilis.

175 reviously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease ac

176 Furthermore, colonization with P. mirabilis promoted intestinal inflammation upon intes

177 Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combi

178 neys from mice transurethrally infected with P. mirabilis were used to prepare template DNA for PCR a

179 sera from mice experimentally infected with P. mirabilis.

180 We conclude that prior infection with P. mirabilis does not protect significantly against homo

181 Etest MEV should not be used with P. mirabilis due to unacceptable analytical performance.