ライフサイエンスコーパス: P. putida

1 P. putida arsH expressed in E. coli conferred resistance

2 P. putida cultivation in lignin-rich media is characteri

3 P. putida expressing the cloned protease IV gene had sig

4 P. putida F1 also responded weakly to cytidine, uridine,

5 P. putida FabB decarboxylates malonyl-ACP and condenses

6 P. putida IsoF utilizes this secretion system not only a

7 P. putida JS110 and JS112, mutant strains which do not e

8 P. putida KT2440 catabolized the d-stereoisomers of lysi

9 P. putida producing protease IV, relative to P. putida w

10 nd (iv) allowed mapping of its network to 82 P. putida sequenced strains revealing functional core th

11 A P. putida G7 nahY mutant grew on naphthalene but was not

12 A P. putida pcaK mutant was defective in its ability to ac

13 BkdR levels were elevated in a P. putida crc mutant, but bkdR transcript levels were th

14 in multiple copies, into the chromosome of a P. putida recipient.

15 the isolates in the P. fluorescens (57) and P. putida (19) groups.

16 However, Pseudomonas aeruginosa and P. putida biofilms remained insensitive to CCCP addition

17 hing expression systems in P. aeruginosa and P. putida, and 'GFP-tagging' Y. pestis.

18 orthologues from Pseudomonas aeruginosa and P. putida, we have determined that these operons encode

19 , two of which - Pseudomonas fluorescens and P. putida - were studied in depth.

20 mined E1beta structures from humans (HU) and P. putida (PP).

21 yl site concentrations for S. oneidensis and P. putida samples when grown in the TSB medium.

22 ter sp. strain ADP1, P. aeruginosa PAO1, and P. putida P111.

23 d V. fischeri moved parallel to the wall and P. putida and E. coli presented a stable movement parall

24 ved among environmental pseudomonads such as P. putida KT2440, P. fluorescens PfO1 and P. fluorescens

25 Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor p

26 fprA expression in both P. putida and P. aeruginosa was found to be regulated by

27 essary for effective sulfate assimilation by P. putida and that the effect of finR mutation on PDTC p

28 c) L-arginine influence biofilm formation by P. putida through changes in c-di-GMP content and altere

29 wall chemical editing by CAN is licensed by P. putida BSAR, a broad-spectrum racemase which catalyse

30 ion by higher PEf1 propagation was offset by P. putida lysis, which decreased stress from interspecie

31 ecreased survival of desiccation not only by P. putida but also by Pseudomonas aeruginosa PAO1 and Ps

32 are located on the EPS molecules produced by P. putida.

33 , each of these amino acids was racemized by P. putida KT2440 enzymes.

34 ) show that nano-Se particles synthesized by P. putida have a size range of 100 to 500 nm and that th

35 ts on the physiology and survival of certain P. putida strains throughout their natural history.

36 logous to sequences present in the completed P. putida KT2440 genome sequence or plasmid pWWO sequenc

37 In contrast, P. putida Idaho demonstrated an increase in phospholipid

38 of the iron-sulfur clusters in the different P. putida FNR proteins influence their reactivity with O

39 e, overexpression of arsH1 and arsH2 endowed P. putida with a high tolerance to the oxidative stress

40 ronucleotides and fluorosugars in engineered P. putida is demonstrated with mineral fluoride both as

41 Transcriptome analysis of DIMBOA-exposed P. putida identified increased transcription of genes co

42 Following o-xylene exposure, P. putida MW1200 exhibited a decrease in total phospholi

43 h Green Fluorescent Protein (GFP)-expressing P. putida showed that DIMBOA-producing roots of wild-typ

44 s of genomic information for P. fluorescens, P. putida, and P. stutzeri suggests that the findings re

45 al cells detached from biofilm (over 70% for P. putida and approximately 40% for polysaccharide produ

46 ow FFF and MALDI-TOF MS was demonstrated for P. putida and E. coli.

47 bon sources (acetate for E. coli, oleate for P. putida) that elevate steady-state ATP levels and boos

48 nstruction and new computable phenotypes for P. putida, which can be leveraged as a first step toward

49 A transport, and they were also required for P. putida to have a chemotactic response to 4-HBA.

50 s that do not serve as growth substrates for P. putida F1.

51 inellus sp. (5.1 x 10(-2) mM d(-1)) than for P. putida (2.32 mM d(-1)).

52 slightly motile, stationary-phase cells from P. putida G7 were mobilized effectively, but the activel

53 The structure of a QuiC1 enzyme from P. putida reveals that the protein is a fusion of two di

54 early biofilm formation, HSLs extracted from P. putida and pure C(12)-HSL were added to 6-h planktoni

55 he octapeptide in case of pseudobactins from P. putida ATCC 39167 is Chr-Ser(1)-Ala(1)-AcOHOrn-Gly-Al

56 unlike PchF obtained from PCMH purified from P. putida, FAD was bound noncovalently.

57 d for the homologous mandelate racemase from P. putida, also a member of the enolase superfamily whos

58 of Ppmt-2 sigma70 with that of sigma70 from P. putida strain G1 shows that the two proteins differ i

59 wo such omega-TAs (TA_5182 and TA_2799) from P. putida KT2440 strain were overexpressed and purified

60 l subunits (Mr = 142,000), whereas that from P. putida is composed of two functionally different prot

61 The N-DBP yield from P. putida EPS was two times higher than that of P. aerug

62 Functional P. putida and P. aeruginosa transporters were identified

63 Furthermore, P. putida cells containing the structurally related AlkB

64 GE P. putida showed high arsenic methylation and volatiliza

65 le expression of an arsM-gfp fusion gene (GE P. putida), which was inserted into the bacterial chromo

66 that was administered to the patients, grew P. putida with a pulsed-field gel electrophoresis (PFGE)

67 In P. putida, all these genes (with the exception of pcaH,G

68 ed as the source of the ion near m/z 7684 in P. putida.

69 higher concentrations by reproducing also in P. putida (7.2 +/- 0.4 vs 6.0 +/- 1.0 log10PFU/mL).

70 hat has been described in other bacteria, in P. putida these proteins seem not to be directly respons

71 sferable Hg(R) captured to the chromosome in P. putida A simple mathematical model suggests these dif

72 on rates and pellicle biofilm development in P. putida GB-1, which has implications for toxic metal b

73 Homologous enzymes in P. putida and A. tumefaciens were identified based on a

74 f genetic tools and epistasis experiments in P. putida, we uncovered an 'upstream' cascade of three c

75 KAS III enzymes are not essential for FAS in P. putida F1, we sought the P. putida initiation enzyme

76 X: free linolenic acid accumulated faster in P. putida BTP1-treated plants than in control.

77 lso observed to occur at high frequencies in P. putida PaW340.

78 nt arrangement of cym, cmt, and tod genes in P. putida F1.

79 ate of turnover was significantly greater in P. putida Idaho than in P. putida MW1200.

80 ting wound outcome, including an increase in P. putida sequence reads.

81 f the known stress tolerance traits known in P. putida but also recognizes the capacity of this bacte

82 aromatic compounds are intimately linked in P. putida.

83 for efficient ethylene glycol metabolism in P. putida KT2440.

84 am of the tod (toluene catabolism) operon in P. putida F1.

85 in the same orientation as the cmt operon in P. putida F1.

86 P. fluorescens allowed pQBR57 to persist in P. putida via source-sink transfer dynamics.

87 have a role in polar flagellar placement in P. putida.

88 use polyvalence enhanced PEf1 propagation in P. putida.

89 The reaction with proteins in P. putida EPS multiplied both the time and the monochlor

90 formation regulation by the Rsm proteins in P. putida.

91 BR57 by nontransferable chromosomal Hg(R) in P. putida was slowed in coculture.

92 57's lower intraspecific conjugation rate in P. putida By contrast, in two-species communities, both

93 llular metabolism and c-di-GMP signalling in P. putida.

94 romoter with a consensus FNR-binding site in P. putida and E. coli strains expressing only one FNR pr

95 we revealed the catabolic pathway for SQ in P. putida SQ1 through differential proteomics and transc

96 ificantly greater in P. putida Idaho than in P. putida MW1200.

97 th, which is the first known phage infecting P. putida S12, a strain increasingly used as a synthetic

98 Ppu12 derivative introduced exogenously into P. putida PP3 via the suicide donor pAWT50 resulted in s

99 s C, plasmid concentration of 0.8 ng/microl, P. putida UWC1 cell concentration of 2.5 x 10(9) CFU (co

100 Finally, benzoate represses the ability of P. putida to transport 4-hydroxybenzoate (4-HBA) by prev

101 ic architecture that affords adaptability of P. putida to divergent carbon substrates and highlight r

102 catalase probe, generated by PCR analysis of P. putida genomic DNA with oligomers based on typical ca

103 ly expressed following initial attachment of P. putida.

104 ctively motile, exponentially grown cells of P. putida G7 were not mobilized.

105 ry, but not in exponentially grown, cells of P. putida G7.

106 As a proof of principle, induced cultures of P. putida KT2440 producing an EGFP-fused model protein b

107 HSL were added to 6-h planktonic cultures of P. putida, and cell extracts were analyzed by 2-D gel pr

108 The results also suggest the degree of P. putida's preference in channeling carbon towards PHA

109 A derivative of P. putida with both arsH genes deleted is sensitive to M

110 on, recovers the mean-square displacement of P. putida if the two distinct swimming speeds are taken

111 that the bimodal turn angle distribution of P. putida reduced collisions with obstacles in porous me

112 on rates we observe competitive exclusion of P. putida.

113 rsH1 and (to a lesser extent) arsH2 genes of P. putida KT2440 strengthened its tolerance to both inor

114 on yields pyruvate, which supports growth of P. putida, and 3-sulfolactaldehyde (SLA), which is oxidi

115 ptake processes in the genome-scale model of P. putida.

116 Chemotaxis assays confirmed motility of P. putida towards DIMBOA.

117 Chromosomal mutants of P. putida deficient in catalase C, obtained by gene inte

118 aize attract significantly higher numbers of P. putida cells than roots of the DIMBOA-deficient bx1 m

119 regulates the plasmid-borne pheBA operon of P. putida PaW85, which is involved in phenol catabolism.

120 The bkd operons of P. putida and P. aeruginosa encode the inducible multien

121 We investigated the in vitro persistence of P. putida in heparinized saline: even under refrigerated

122 1 g/L nZVI induced a persistent phenotype of P. putida F1 as indicated by smaller colony morphology,

123 The annotated proline racemase ProR of P. putida KT2440 showed negligible activity with either

124 Microbial growth rates of P. putida in subsurface environments can only be accurat

125 A screen of P. putida F1 mutants, each lacking one of the genes enco

126 ficant increase in the copper sensitivity of P. putida KT2440 under the conditions tested.

127 revealed that the default metabolic state of P. putida KT2440 is characterized by a slight catabolic

128 However, most strains of P. putida cannot metabolize pentose sugars derived from

129 of different samples with respect to that of P. putida.

130 of nano-Se and the metabolic versatility of P. putida offer the opportunity to use this model organi

131 .e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction a

132 Single inoculations with R. irregularis or P. putida had differential growth effects on both cultiv

133 r single inoculations with R. irregularis or P. putida, only the cultivar with high mycorrhizal compa

134 host and was also found to infect four other P. putida strains.

135 gene clusters were found in genomes of other P. putida strains, in other gamma-Proteobacteria, and in

136 rvivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediate

137 We show here that recombinant P. putida glyoxalase I is an active dimer (kcat approxim

138 ed on such quantum formulation, representing P. putida's PHA biosynthesis with respect to external C/

139 Analysis of phospholipid biosynthesis showed P. putida Idaho to have a higher basal rate of phospholi

140 s with more competing soil bacteria species, P. putida lysis was less critical in mitigating interspe

141 , using cocultures of two bacterial species, P. putida and P. veronii.

142 a protease IV-negative Pseudomonas species, P. putida.

143 clustered with plant growth-promoting strain P. putida W619 (Strain M2), while the third isolate repr

144 utida Idaho, and a solvent-sensitive strain, P. putida MW1200, were examined in terms of phospholipid

145 The responses of a solvent-tolerant strain, P. putida Idaho, and a solvent-sensitive strain, P. puti

146 Like other prokaryotic swimmers, P. putida exhibits a motion pattern dominated by persist

147 The results presented here demonstrate that P. putida undergoes a global change in gene expression f

148 A transport and chemotaxis demonstrates that P. putida has a chemoreceptor that differs from the clas

149 These data show that P. putida FabB, unlike the paradigm E. coli FabB, can ca

150 Our results show that P. putida is able to reduce selenite aerobically, but no

151 the initial Mn(II) concentration, shows that P. putida fine-tunes the regulation of multiple Mn oxida

152 These results suggest that P. putida Idaho has a greater ability than the solvent-s

153 The P. putida enzyme had a Km for NG of 52 +/- 4 microM, a K

154 The P. putida gene, xenA, encodes a 39,702-Da monomeric, NAD

155 A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced.

156 We genetically engineered the P. putida KT2440 with stable expression of an arsM-gfp f

157 when it expressed the five enzymes from the P. putida operon.

158 ayer between bulk and biofilm surface in the P. putida biofilm compared to those of P. aeruginosa bio

159 erent diversity in pentose catabolism in the P. putida group and may provide alternative hosts for bi

160 view of the pentose sugar catabolism in the P. putida group.

161 Like the P. putida enzyme, hh4-OT requires the amino-terminal pro

162 her persistence in the middle section of the P. putida biofilm compared to the P. aeruginosa biofilms

163 ntial for FAS in P. putida F1, we sought the P. putida initiation enzyme and unexpectedly found that

164 ics by numerical integration showed that the P. putida enzyme produced an approximately 2-fold molar

165 ruginosa helicase and significantly with the P. putida helicase, whereas deletion of amino acids 71-8

166 ced activity in vitro, particularly with the P. putida helicase.

167 loped an adenoviral vector inserted with the P. putida methioninase (MET) gene (rAd-MET).

168 BLAST analyses conducted with the P. putida xenA and the P. fluorescens xenB sequences dem

169 tor for Pnao is unknown; however, within the P. putida S16 genome, pnao forms an operon with cycN and

170 These P. putida cell extracts produced a protein with the same

171 P. putida producing protease IV, relative to P. putida with the vector alone, caused a threefold incr

172 We report that although two P. putida F1 fabH genes (PpfabH1 and PpfabH2) both encod

173 from garden soil under a tomato plant using P. putida S12 as a host and was also found to infect fou

174 mass yield of the evolved D-xylose utilizing P. putida strain.

175 nstrated that these enzymes are induced when P. putida is grown in the presence of 3-chlorobenzoate,

176 Fractionation obtained with P. putida GPo1 was similar to acid hydrolysis and M. aus

177 emoval, the measured sulfhydryl sites within P. putida samples was 34.9 +/- 9.5 mumol/g, and no sulfh