ライフサイエンスコーパス: PAS domain

1 a diminished inhibitory interaction with the PAS domain.

2 s in Escherichia coli with an FAD-containing PAS domain.

3 will adopt a fold similar to the ubiquitous PAS domain.

4 e periplasmic (PP) domain, and a cytoplasmic PAS domain.

5 nges in the N-terminal cap (Ncap) of the VVD PAS domain.

6 , which has both a HAMP domain and a sensory PAS domain.

7 vin adenine dinucleotide cofactor bound to a PAS domain.

8 -sheet interface between the monomers of the PAS domain.

9 of N- or C-terminal helices attached to the PAS domain.

10 portant for stabilizing the structure of the PAS domain.

11 at GAC63 interacts with AHR through its bHLH-PAS domain.

12 face for the interaction with the HIF-2alpha PAS domain.

13 AMP domain that abolished FAD binding to the PAS domain.

14 he Aer sensor is an N-terminal, FAD-binding, PAS domain.

15 could be activated by ligand binding to the PAS domain.

16 redox potential via FAD bound to a cytosolic PAS domain.

17 vs) that recognize different epitopes on the PAS domain.

18 lators of hERG that specifically bind to the PAS domain.

19 channels directly through the binding to the PAS domain.

20 code an [FeFe]-hydrogenase with a C-terminal PAS domain.

21 noticeably shorter than those of the typical PAS domains.

22 in both isolated and tandem arrangements of PAS domains.

23 olution of ligand recognition and binding by PAS domains.

24 tem via the FAD cofactor associated with its PAS domains.

25 allosteric switching that is conserved among PAS domains.

26 rotation of the heme-binding beta H-NOX and PAS domains.

27 asparagine receptor McpB revealed two tandem PAS domains.

28 ed HIF-2alpha ubiquitination at the bHLH and PAS domains.

29 e closely resembles prokaryotic heme-binding PAS domains.

30 rotein with the mixed alpha/beta topology of PAS domains.

31 ns containing the basic-helix-loop-helix and PAS domains.

32 are regulated by an N-terminal Per-Arnt-Sim (PAS) domain.

33 PYP is also the prototypical Per-Arnt-Sim (PAS) domain.

34 the presence of an N-terminal Per-Arnt-Sim (PAS) domain.

35 factor 1 subunit alpha (HIF1A), endothelial PAS domain 1 protein (EPAS1, also called HIF2A), CA9, so

36 entrainment requires expression of Neuronal PAS domain 2 (Npas2) and Period2 (Per2) genes, component

37 The circadian transcription factor neuronal PAS domain 2 (NPAS2) is linked to psychiatric disorders

38 YybT homologs contain a small Per-ARNT-Sim (PAS) domain (~80 amino acids) that can bind b-type heme

39 tio via binding of NAD(+) to the kinase in a PAS domain A-dependent manner.

40 The PAS domain (a sensory domain named after three proteins

41 OX domains (one with heme, one without), two PAS domains, a coiled-coil domain, and two cyclase domai

42 In oxygen-sensing PAS domains, a conserved polar residue on the proximal s

43 ygen binding (H-NOX) domain, a Per/ARNT/Sim (PAS) domain, a coiled-coil (CC) domain, and a catalytic

44 ase predicted to have multiple Per/Arnt/Sim (PAS) domains, a histidine kinase domain and a C-terminal

45 sequence level, the structures of dozens of PAS domains across a broad section of sequence space hav

46 implications for the molecular mechanism of PAS domain activation and indicate that stimulus-induced

47 PAS domain activation often involves conformational chan

48 or function by the interaction with the bHLH-PAS domain (AD3) of p160 coactivators.

49 both PAS and GAF domains, rather than in the PAS domain alone as in wild-type BphPs.

50 Both domains adopt the typical PAS domain alpha/beta topology and are structurally simi

51 taining an ACT domain, a connecting helix, a PAS domain and a C-terminal helix.

52 ge-charge interaction between Arg(56) of the PAS domain and Asp(803) of the cNBH domain, as well an a

53 rongly protected two regions of the sGCbeta1 PAS domain and caused local structural relaxation in oth

54 d the region encoding residues 14-119 of the PAS domain and found 72 aerotaxis-defective mutants, 24

55 In ReFixL, the core heme-containing PAS domain and kinase region is preceded by an N-termina

56 co-factor located within the amino-terminal PAS domain and responds to the fixed nitrogen status by

57 ctivity was also shown to depend on the WalK PAS domain and to limit cross-talk and the recovery of W

58 toplasmic BdlA protein harboring two sensory PAS domains and a chemoreceptor domain, TarH.

59 rotein assumes the global fold common to all PAS domains and dimerizes via a hydrophobic interface.

60 late these changes to those in other dimeric PAS domains and discuss the role of quaternary structura

61 ose described in other crystal structures of PAS domains and identifies a conserved dimerization moti

62 t establish DNA binding and the stability of PAS domains and pockets.

63 new insight into the architecture of tandem PAS domains and suggests specific residues that may play

64 or functional interactions between the N-Cap/PAS domains and the cNBH domain.

65 domain, which is composed of a Per-Arnt-Sim (PAS) domain and a PAS-cap domain, whereas the carboxy-te

66 RmcA contains four Per-Arnt-Sim (PAS) domains and domains with the potential to catalyze

67 n lesions mimic the 'signal-on' state of the PAS domain, and therefore may be markers for the signal-

68 the complex formed in solution by these two PAS domains, and confirm the validity of this model usin

69 hich dimerize via basic helix-loop-helix and PAS domains, and recruit coactivators via HIF-alpha C-te

70 s Sma0113, an HWE histidine kinase with five PAS domains, and Sma0114, a CheY-like response regulator

71 op-helix (bHLH) domain and two Per-Arnt-Sim (PAS) domains, and HdHIF-1alpha has a oxygen-dependent de

72 oiled-coil platform upon which the H-NOX and PAS domains are assembled.

73 In the bacterial kingdom, PAS domains are commonly positioned at the amino terminu

74 PAS domains are defined by a small number of conserved r

75 The beta1 H-NOX and alpha1 PAS domains are in contact and form the core signaling c

76 nt and could differ depending on whether the PAS domains are isolated or are part of a full-length pr

77 PAS domains are often dimeric and act as versatile senso

78 Multiple PAS domains are often found within a single protein.

79 Physically, HIF1alpha HLH and PAS domains are required for its interaction with STAT3

80 Per/Arnt/Sim (PAS) domains are ubiquitous sensors in thousands of spec

81 ted ERG and ELK K(+) channels and places the PAS domain as a new target for drug discovery in EAG and

82 ng spectroscopy and kinetics, identified the PAS domain as the location for heme binding.

83 lK(Spn) phosphatase activity depended on the PAS domain as well as residues in the DHp domain.

84 ed by the N-terminal N-Cap and Per-Arnt-Sim (PAS) domains, as well as the C-terminal cyclic nucleotid

85 omplex containing the mouse CLOCK:BMAL1 bHLH-PAS domains at 2.3 A resolution.

86 The basic helix-loop-helix PAS domain (bHLH-PAS) transcription factor CLOCK:BMAL1 (

87 In other proteins, PAS domains bind ligands and modulate effector domains.

88 -HAMP function, we determined that the Aer-2 PAS domain binds penta-co-ordinated b-type haem and that

89 Spectroscopy reveals that whereas the PAS domain binds to both the ferric and ferrous forms of

90 nscription factor; the AhR Per-AhR/Arnt-Sim (PAS) domain binds ligands.

91 arginine and tyrosine residues of the upper PAS domain but not in any residues of the lower PAS doma

92 similar to those of other flavin-containing PAS domains, but homodimeric interactions in other struc

93 e of the N-cap (residues 1 to 19) in the Aer PAS domain by missense and truncation mutagenesis.

94 annel function through ligand binding to the PAS domain can be attained.

95 Our data reveal that the ubiquitous PAS domain can make the transition from sensor to enzyme

96 ural similarity, it is not known whether the PAS domain can regulate EAG channel function via ligand

97 f ligand affinity and signal transduction by PAS domains can be direct or indirect.

98 he eag domain, consisting of a Per-ARNT-Sim (PAS) domain capped by a short sequence containing an amp

99 g interactions as observed for several other PAS domain complexes.

100 Because disruption of individual PAS domains compromise HIF function independent of the m

101 PAS domain containing protein kinase (Pask) is an evolut

102 The basic helix-loop-helix (bHLH) -PAS domain containing transcription factors CLOCK and BM

103 ins, CetA (HAMP domain containing) and CetB (PAS domain containing).

104 e sensor domains are the first structures of PAS domains containing covalently bound heme.

105 nvironmental ligand-dependent, per ARNT-sim (PAS) domain containing bHLH transcription factor that me

106 investigation of the role of the endothelial PAS domain-containing protein 1 (EPAS1), a regulatory al

107 -gamma, coactivator 1 (Pgc-1alpha), neuronal PAS domain-containing protein 2 (Npas2), and retinoic ac

108 In this review, we summarize the roles of PAS domain-containing proteins in mammals.

109 Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) is an evolu

110 eurospora circadian clock, the PER-ARNT-SIM (PAS) domain-containing transcription factor, WHITE COLLA

111 cloche gene and discovered that it encodes a PAS-domain-containing bHLH transcription factor, and tha

112 esis analogous to the functions performed by PAS-domain-containing regulatory proteins found in compl

113 We show that upon citrate binding, the PAS domain contracts, resulting in a shortening of the C

114 Here we show that CRY1 binds directly to the PAS domain core of CLOCK:BMAL1, driven primarily by inte

115 ponicum is a PAS sensor protein in which two PAS domains covalently linked to a histidine kinase doma

116 tilis, we constructed a series of systematic PAS domain deletion mutants and analyzed their activitie

117 It consists of an N-terminal heme-bound PAS domain, denoted bjFixLH, and a C-terminal histidine

118 The more C-terminal PAS domain, denoted bjFixLH, contains a heme cofactor th

119 The crystal structure of the MmoS tandem PAS domains, designated PAS-A and PAS-B, has been determ

120 odules, such as the widespread Per-ARNT-Sim (PAS) domains, detect signals and, in response, regulate

121 esent homology models of the murine AhR:ARNT PAS domain dimer developed using recently available X-ra

122 ion, early doors (Edo), in the PER-ARNT-SIM (PAS) domain dimerization region of period 2 (PER2) (I324

123 hat permit small-molecule binding, including PAS domain encapsulated sites and an interfacial cavity

124 domain but not in any residues of the lower PAS domain exhibited a chemotactic defect in both swarm

125 onto the transcription factor alongside the PAS domains, extending above the DNA-binding bHLH domain

126 t of 63 PAS structures demonstrates that the PAS domain family forms structural clades on the basis o

127 ll-molecule metabolites is a hallmark of the PAS domain family.

128 ive yellow protein (PYP), a prototype of the PAS domain family.

129 o be distinct from the superficially similar PAS domain fold.

130 l aromatic hydrocarbon-sensing Per-Arnt-Sim (PAS) domain, followed by an autokinase domain, a respons

131 Sma0113 may use its five PAS domains for redox level or energy state monitoring a

132 of erWalK revealed a canonical Per-Arnt-Sim (PAS) domain for signal sensing.

133 PAS domains, found in diverse multi-domain proteins from

134 Here we present crystal structures of PAS domain fragments of mPER1 and mPER3 and compare them

135 of the heterodimer formed by the C-terminal PAS domains from the HIF2alpha and ARNT subunits of the

136 The universal Per-ARNT-Sim (PAS) domain functions as a signal transduction module in

137 The PAS domain has also been implicated in the assembly and

138 ytic activity, to our knowledge no enzymatic PAS domain has been found.

139 at least twice in evolutionary history, the PAS domain has been lost and it is omitted by alternate

140 ired to mediate other HIF functions in which PAS domains have been implicated.

141 el of voltage gating, but that the N-Cap and PAS domains have different roles in these channels.

142 The crystal structure of the heme-binding PAS domain in the ferric, ligand-free form, in compariso

143 of the environmental sensing role played by PAS domains in a wide range of proteins, including other

144 Given the essential role of PAS domains in forming active HIF heterodimers, these re

145 KinA contains three PAS domains in its amino-terminal sensor domain, which a

146 To characterize the role of the three PAS domains in KinA, the major sporulation kinase in Bac

147 he PAS domain is structurally similar to the PAS domains in non-ion channel proteins, where these dom

148 , as also borne out by comparison to several PAS domains in which mutations leading to disruption of

149 EAG channels contain a Per-Arnt-Sim (PAS) domain in their intracellular N-terminal region.

150 show that both antibodies, on binding to the PAS domain, increase the total amount of current that pe

151 on, we propose a model for the mode of multi-PAS domain interaction in bHLH-PAS transcriptional activ

152 The second PAS domain interacts with the asymmetrically partitioned

153 Putting the extracytoplasmic PAS domain into context of the membrane-embedded CitA co

154 Intriguingly, the hallmark PAS domain is dispensable for LIN-42 function in transge

155 antitative Western blotting to show that the PAS domain is not required for normal channel traffickin

156 However, when the PAS domain is present, the N-Cap amphipathic helix must

157 abilization of full-length proteins when the PAS domain is present.

158 The PAS domain is structurally similar to the PAS domains in

159 An FAD-binding PAS domain is the sensor for Aer and a HAMP domain inter

160 The results showed that any one of the three PAS domains is sufficient to maintain the kinase activit

161 The Per-ARNT-Sim (PAS) domain is a conserved alpha/beta fold present withi

162 The Per-Arnt-Sim (PAS) domain is a ubiquitous protein module with a common

163 The Per-Arnt-Sim (PAS) domain is conserved across the kingdoms of life and

164 the Helix-Loop-Helix (HLH) and PER-ARNT-SIM (PAS) domains, is needed to convert the AhR into its tran

165 s that, in contrast to previous studies, the PAS domain itself is extended by approximately 3 nm (at

166 elices, with RsbQ hydrolase activity and the PAS domain jointly comprising a positive sensing module

167 Mutations located on one face of the PAS domain (K28E, F29L, N33T, R56Q, and M124R) caused de

168 eractions between the region upstream of the PAS domain knot and the bilin A and B pyrrole rings.

169 However, its Per/Arnt/Sim (PAS) domain, knot region, and helical spine show distinc

170 small percentage of hERG channels containing PAS domain LQT2 mutations (hERG PAS-LQT2) have been char

171 and CW-biased mutations suggest that the Aer PAS domain may engage in two different interactions with

172 These include the PAS-domain metabolic sensor kinase Psk2, the mitochondri

173 S-like domains do not match sequence-derived PAS domain models, and thus their distribution across th

174 reconstituted on liposomes, we show that one PAS domain modulates kinase activity in a CckA density-d

175 A deletion of the N-terminus PAS domain, mutation R4AR5A or the LQT2-causing mutation

176 We used a genetically encoded hERG PAS domain (NPAS) to examine whether channel dysfunction

177 Per-Arnt-Sim (PAS) domains occur in proteins from all kingdoms of life

178 the solution structure of the corresponding PAS domain of ARNT and show that it utilizes a very simi

179 ipsychotic medication, binds to the isolated PAS domain of EAG channels and inhibits currents from th

180 All these mutations were in the cytoplasmic PAS domain of EvgS, and were shown to be solely responsi

181 The N-terminal PAS domain of FixL contains a histidine-coordinated heme

182 We determined the solution structure of the PAS domain of GtYybT from Geobacillus thermodenitrifican

183 In addition, we expressed the Cap and PAS domain of hERG3 in Escherichia coli and, using spect

184 mined the crystal structure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A

185 ellular redox is monitored by the N-terminal PAS domain of NifL which contains an FAD cofactor.

186 BDSF is shown to bind to the PAS domain of RpfR with high affinity and stimulates its

187 pport a model whereby the disulfide bond and PAS domain of SrrB sense and respond to the cellular red

188 the Aer dimer stabilized FAD binding to the PAS domain of the cognate monomer.

189 ind an internal cavity within the C-terminal PAS domain of the HIF-2alpha subunit.

190 Here it is shown that the cytoplasmic PAS domain of this multi-domain transmembrane kinase is

191 d kinase region is preceded by an N-terminal PAS domain of unknown function and followed by a C-termi

192 e report here that CoCoA also binds the bHLH-PAS domains of AHR and ARNT and functions as a potent pr

193 We have previously shown that the C-terminal PAS domains of an HIF-alpha isoform (HIF-2alpha) and ARN

194 S1P specifically binds to the PAS domains of HIF-1alpha.

195 mains bind HIF-2alpha and that both bHLH and PAS domains of HIF-2alpha interacted with ORF34.

196 However, the PAS domains of KCNH channels are orphan receptors.

197 primary structure within the two N-terminal PAS domains of LovhK have distinct sensory roles under s

198 to recent models, our data reveal that both PAS domains of the HIF-alpha subunit are necessary for h

199 Flavin-based PAS domains of this type have also been referred to as L

200 and its M domain bound to the Per-Arnt-Sim (PAS) domain of apo-sGC-beta1(1-359), which lies adjacent

201 eract directly with the second PER-ARNT-SIM (PAS) domain of ARNT (ARNT PAS-B).

202 The C-terminal Per-ARNT-Sim (PAS) domain of HIF2alpha (HIF2alpha PAS-B) contains a pr

203 racytoplasmic, citrate-sensing Per-Arnt-Sim (PAS) domain of HK CitA are identical for the isolated do

204 domain used to associate with the analogous PAS domain on its heterodimeric bHLH-PAS partner HIF-2al

205 runcation of the N-Cap domain, Per-Arnt-Sim (PAS) domain, or both in K(V)10.2 abolished the current a

206 onal enzyme and that the most amino-terminal PAS domain (PAS-A) plays an important role in sensing th

207 nsible for redox sensing, whereas the second PAS domain, PAS2, has no apparent cofactor and its funct

208 Per-Arnt-Sim (PAS) domains play a critical role in signal transduction

209 HIF1A (encoding HIF-1alpha) and endothelial PAS domain protein 1 (EPAS1 encoding HIF-2alpha), inhibi

210 nducible factor (HIF) 1alpha and endothelial PAS domain protein 1 (EPAS1 or HIF2alpha), which are ind

211 subunits, HIF1A (HIF-1alpha) and endothelial PAS domain protein 1 (EPAS1; HIF-2alpha), are overexpres

212 muscle Arnt-like protein 1 (Bmal1), neuronal PAS domain protein 2 (Npas2) and cryptochrome 1 (Cry1),

213 -PAS domain proteins like mammalian neuronal PAS domain protein 2 (NPAS2) and the direct oxygen senso

214 The circadian transcription factor neuronal PAS domain protein 2 (NPAS2) is enriched in reward-relat

215 s recapitulated with a knockdown of neuronal PAS domain protein 2 (NPAS2) specifically in the NAc, de

216 s kaput (Clock) in combination with neuronal PAS domain protein 2 (Npas2), induced severe age-depende

217 Mice missing the gene encoding neuronal PAS domain protein 3 (NPAS3) are devoid of hippocampal n

218 The neuronal PAS domain protein 3 (NPAS3) gene encoding a brain-enric

219 In mice, loss of neuronal PAS domain protein 3 (NPAS3) impairs postnatal hippocamp

220 ew loci, nucleobindin 1 (NUCB1) and neuronal PAS domain protein 4 (NPAS4) (p <6x 10(-6)).

221 Neuronal PAS domain protein 4 (Npas4), a recently discovered IEG,

222 unction of the novel beta-cell IEG, neuronal PAS domain protein 4 (Npas4).

223 he transcription factor (TF) NPAS4 (neuronal PAS domain protein 4) has been found to provide activity

224 transcription factor encoded by endothelial PAS domain protein-1 (EPAS1).

225 wo basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) domain protein subunits, CLOCK and BMAL1.

226 basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor.

227 romoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1.

228 RcoM-1 and BxRcoM-2, are gas-responsive heme-PAS domain proteins like mammalian neuronal PAS domain p

229 Acting through intestinal bHLH-PAS domain proteins Methoprene-tolerant (Met) and Germ c

230 The neuronal PAS domain proteins NPAS1 and NPAS3 are members of the b

231 nisms of AhR transformation, dimerization of PAS domain proteins, and Hsp90 dissociation in activatio

232 anges in PYP plus commonalities shared among PAS domain proteins, we further propose that PAS domains

233 r N-terminal helices are identified in other PAS domain proteins.

234 2 basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) domain proteins-CLOCK and BMAL1.

235 ominant regulatory effect was exerted by the PAS domain proximal to the effector domain.

236 mutants that probe different regions of the PAS domain quantify the anisotropy in stability and chan

237 s interaction is regulated by the PAS kinase PAS domain, raising the possibility that this interactio

238 specific DivL mutants revealed that the DivL PAS domains regulate binding specificity for DivK approx

239 Here, we show that the PAS domain regulates both kinase and phosphatase enzyme

240 proteins, extracytoplasmic sigma factors and PAS-domain regulators.

241 protein variants strongly suggested that the PAS domain residues His74 and Met104 serve as the heme F

242 alanine replacement mutagenesis of the BdlA PAS domain residues previously demonstrated to be essent

243 c channel, with many clinical mutants in the PAS domain resulting in reduced stability of the domain

244 haracterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this pro

245 ne kinase upstream of CtrA, employs a tandem-PAS domain sensor to integrate two distinct spatiotempor

246 PAS domain proteins, we further propose that PAS domains share this conformational mechanism, which e

247 Mutant EAG channels that lack the PAS domain show significantly lower inhibition by CH, su

248 ctural data on natural receptors with tandem PAS domains show that these are predominantly linked by

249 In contrast, absence of the HAMP-PAS domains significantly diminished the phosphatase act

250 In contrast, PAS domains (similar to GAF domains in structure but not

251 ha subunit reveals that it is a haem-binding PAS domain, similar in structure to PAS gas sensors.

252 ts from NMR spectroscopy illustrate how this PAS domain simultaneously mediates interactions with HIF

253 A deletion of the first PAS domain strongly increased the oxygen affinity but es

254 where ligand binding induces alterations in PAS domain structure and subunit association that is rel

255 sensing domain exhibits a dual Per-Arnt-Sim (PAS) domain structure.

256 r within its N-terminal tandem Per-Arnt-Sim (PAS) domains, suggesting that it functions as a redox se

257 a 125-residue prototype of the PER-ARNT-SIM (PAS) domain superfamily of signaling proteins.

258 ithin the first of two amino-terminal tandem PAS domains, termed PAS1 and PAS2.

259 chia coli Aer protein contains an N-terminal PAS domain that binds flavin adenine dinucleotide (FAD),

260 The structure contains a dimer of the NifL PAS domain that is structurally very similar to those de

261 The Aer sensor is a cytoplasmic, N-terminal PAS domain that is tethered to the membrane by a 47-resi

262 volve the hydrophobic pocket within the PER2 PAS domains that in other PAS proteins commonly recogniz

263 a potassium channel contains a Per-Arnt-Sim (PAS) domain that is essential for the unique slow deacti

264 domain (eagD) that contains a Per-Arnt-Sim (PAS) domain that is preceded by a conserved sequence of

265 Thus, in oxygen-sensing PAS domains, the interactions of polar residues with the

266 from Azotobacter vinelandii contains tandem PAS domains, the most N-terminal of which, PAS1, contain

267 e mediated by genes containing Per-Arnt-Sim (PAS) domains, the aryl hydrocarbon receptor (AhR), and A

268 otates, extends and cooperates with a distal PAS domain to bind hypoxia response elements.

269 We developed homology models of the AhR PAS domain to characterize previously observed intra- an

270 for Aer and a HAMP domain interacts with the PAS domain to form an input-output module for signal tra

271 Here we show that ARNT uses a single PAS domain to interact with two coiled coil coactivators

272 exposed hydrophobic patch on the core of the PAS domain to stabilize the structure of this critical g

273 the signal transduction pathway from the Aer PAS domain to the signalling domain.

274 on of allosteric structural changes from the PAS domain to these helices is not clear.

275 which also interact with hsp90 through their PAS domains to control protein partner and small ligand

276 Met and FISC appear to use their PAS domains to form a dimer only in the presence of JH o

277 been shown to use its N-terminal bHLH and/or PAS domains to interact with several transcriptional coa

278 and, more broadly, to the abilities of some PAS domains to regulate their activities in response to

279 Trachealess (Trh) is a PAS domain transcription factor regulating Drosophila tr

280 basic helix-loop-helix (bHLH)-Per-Arnt-Sim (PAS) domain transcription factor BMAL1 is an essential c

281 like for the hERG channel in which N-Cap and PAS domain truncations mainly affected channel deactivat

282 demonstrating the partial unfolding of their PAS domain upon activation.

283 in an orientation orthogonal to that in the PAS domains via a highly conserved motif, including inva

284 e explored the function(s) of the HIF-2alpha PAS domains via mutational analysis.

285 The PAS domain was also critical for RscS activity; substitu

286 Although PAS domains were described as intracellular sensors, rec

287 forms of the R206A and wild-type BjFixL heme-PAS domains were similar, except for a more ruffled porp

288 sults reveal a putative "gating face" in the PAS domain where mutations within this region form funct

289 structure reveals a novel cavity within the PAS domain which contains two water molecules directly c

290 Aer is a flavin adenine dinucleotide-binding PAS domain, which is separated from a HAMP/signaling out

291 We demonstrate that this contraction of the PAS domain, which is well characterized for the isolated

292 t upstream of an N-terminal Period/Arnt/Sim (PAS) domain, which upon removal dramatically accelerates

293 CetB has a PAS domain, while CetA has a predicted transmembrane reg

294 some resemblance to LOV domains, a subset of PAS domains widely involved in signaling.

295 ed that DSF bound to the isolated N-terminal PAS domain with a Kd of 1.4 muM.

296 Together, the results unveil a compact PAS domain with a potential ligand-binding pocket and re

297 ross handshake interaction of the N-terminal PAS domain with alphaF of the opposing subunit.

298 The two PAS domains within ARNT, PAS-A and PAS-B, are essential

299 ional role for transcriptional regulation by PAS domains within bHLH-PAS transcription factors.

300 These results support the importance of PAS domains within KinA and other histidine kinases and