ライフサイエンスコーパス: PCR amplification

1 cific mtDNA from wastewater before and after PCR amplification.

2 s without the need for long culture steps or PCR amplification.

3 nts suffer from distortions occurring during PCR amplification.

4 e amplification of wild type DNA during LATE-PCR amplification.

5 o the start of the exponential phase of LATE-PCR amplification.

6 challenges for studies based on conventional PCR amplification.

7 roximately 36 molecules per sample), without PCR amplification.

8 ld and mutant DNA in genomic samples without PCR amplification.

9 gene was confirmed by failure of 3'- and 5'-PCR amplification.

10 ve patients were also positive for H. pylori PCR amplification.

11 f the formation of chimeric sequences during PCR amplification.

12 s to heat the sample and perform a real-time PCR amplification.

13 at low copy numbers intrinsic to exponential PCR amplification.

14 s compatible with downstream microchip-based PCR amplification.

15 of purifying both RNA and DNA for downstream PCR amplification.

16 dundancy that arises from multiple rounds of PCR amplification.

17 horesed to a recovery chamber for subsequent PCR amplification.

18 s genotyping accuracy and removes noise from PCR amplification.

19 or dynamic solid phase extraction (dSPE) and PCR amplification.

20 identical length can also be generated using PCR amplification.

21 The s type and the m type were determined by PCR amplification.

22 strand displacement amplification (SDA), and PCR amplification.

23 mited by the extraction procedure and not by PCR amplification.

24 3 days prior to DNA extraction and real-time PCR amplification.

25 tides and that operates without the need for PCR amplification.

26 sulting in rapid (55-s cycles) and efficient PCR amplification.

27 ime of 1.5 h and without the need for sample PCR amplification.

28 croarray following reverse transcription and PCR amplification.

29 t the lower temperatures that exist prior to PCR amplification.

30 the limitations associated with conventional PCR amplification.

31 iosynthesis; it can be completed 45 min post-PCR amplification.

32 being converted into a sequencing library by PCR amplification.

33 olecule, allowing real-time detection during PCR amplification.

34 f target BWAs in spiked sewage samples after PCR amplification.

35 o a 550-nl chamber for rapid target sequence PCR amplification.

36 omavirus 2 (EcPV2) infection, detected using PCR amplification.

37 ing an Abbott platform, and HCV subtype with PCR amplification.

38 sed purification, without the need to employ PCR amplification.

39 PCR chambers enables real-time monitoring of PCR amplification.

40 es we show that the latter are not skewed by PCR amplification.

41 pendent experiments and by performing direct PCR amplification.

42 tic processes like reverse transcription and PCR amplification.

43 ause of the stochastic nature of exponential PCR amplification.

44 large quantities of marked DNA to be made by PCR amplification.

45 extracted from bacterial culture and without PCR amplification.

46 artifacts such as template switching during PCR amplification.

47 ic microorganisms and eliminated the need of PCR amplifications.

48 l (TRAP) based on polymerase chain reaction (PCR) amplification.

49 of extraction and polymerase chain reaction (PCR) amplification.

50 o inform robust primer design when employing PCR amplification, a factor that is often lacking when w

51 typically employ polymerase chain reaction (PCR) amplification after bisulfite conversion, thereby l

52 PCR amplification, agarose gel electrophoresis, and sequ

53 tive transcription factors were generated by PCR amplification and assessed for transcriptional activ

54 These ESTs allow for specific PCR amplification and broad coverage across the genome,

55 influence of probe length and sample age on PCR amplification and co-expression of candidate genes o

56 tion and an m2000rt instrument for real-time PCR amplification and detection.

57 a(KPC) genes and was combined with selective PCR amplification and DNA sequencing for complete charac

58 The i type was identified by PCR amplification and DNA sequencing.

59 A challenging target sequence for both PCR amplification and electrochemical detection allowed

60 ID system is a novel technology that couples PCR amplification and electrospray ionization-mass spect

61 PCR amplification and enzyme digestions were used to ana

62 Targeted PCR amplification and high-throughput sequencing (amplic

63 The combined use of emulsion PCR amplification and high-throughput sequencing allows

64 PCR amplification and high-throughput sequencing theoret

65 DNA in situ ligation with a bivalent linker, PCR amplification and high-throughput sequencing.

66 tion with a bridge linker, Tn5 tagmentation, PCR amplification and high-throughput sequencing.

67 sduced with a lentiviral vector, followed by PCR amplification and Illumina sequencing of virus-genom

68 Masking DNA lacks primer regions for PCR amplification and is typically taken in excess to th

69 A system that automatically performs the PCR amplification and microchip electrophoretic (ME) sep

70 nomic DNA from bacteria without the need for PCR amplification and molecular labeling is described.

71 We performed PCR amplification and next generation deep immune repert

72 DNA synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) t

73 hes to molecular diagnostics rely heavily on PCR amplification and optical detection methods which ha

74 ver salmon testes DNA as it had no effect on PCR amplification and provided the lowest relative expan

75 PCR amplification and pyrosequencing was carried out to

76 we demonstrate PCR-chimera formation during PCR amplification and reference alignment bias are pitfa

77 Genomic PCR amplification and resequencing indicated that the ge

78 ongenital infection was determined by direct PCR amplification and restriction fragment length polymo

79 Also, based on PCR amplification and sequence analysis of the DNA joint

80 e present here an analysis of the utility of PCR amplification and sequence analysis of the hypervari

81 nguishing true nucleotide substitutions from PCR amplification and sequencing artifacts.

82 % of the Acidobacteria Group 1 sequences, by PCR amplification and sequencing of DNA from soil.

83 al masses, previously formally identified by PCR amplification and sequencing of internal transcribed

84 ction of Pathogenic Fungi were identified by PCR amplification and sequencing of internal transcribed

85 We used PCR amplification and sequencing of retroviral fragments

86 as developed to detect and identify, through PCR amplification and sequencing of the 16S-rRNA gene, t

87 2 isolates, and bla(KPC-3) was identified by PCR amplification and sequencing of the amplicon.

88 biochemical tests (AUXACOLOR2) and following PCR amplification and sequencing of the D1-D2 portion of

89 Further, we show that PCR amplification and sequencing of the D1/D2 region rel

90 PCR amplification and sequencing of the D1D2 and interna

91 r the MICs of cefpodoxime were >4 microg/ml, PCR amplification and sequencing of the ESBL and pAmpC g

92 PCR amplification and sequencing of the most variable N-

93 ication compared with ribosomal markers, low PCR amplification and sequencing success eliminated them

94 a null allele (Lim2(Gt)), as verified by RT-PCR amplification and sequencing, RNA blotting, immunobl

95 ified from the DMNQ-resistant VSMC clones by PCR amplification and sequencing.

96 PCR amplification and serological testing identified the

97 suitable for downstream applications such as PCR amplification and shotgun sequencing.

98 The use of more than 20 cycles of PCR amplification and/or more than 50 ng of starting gen

99 By RT-PCR amplifications and DNA sequencing, numerous in-frame

100 owever, biases in polymerase chain reaction (PCR) amplification and in cloning can skew the results.

101 ional strategy of polymerase chain reaction (PCR) amplification and sequencing of individual exons.

102 g unmethylated Cs to Us and then to Ts after PCR amplification) and next generation sequencing (NGS)

103 ecules of miRNA per 30 muL of sample without PCR amplification, and can be operated within the dynami

104 followed by fragmentation, cDNA generation, PCR amplification, and deep sequencing.

105 beta-joining(J)beta fingerprint generated by PCR amplification, and determined the primary structure

106 DNA extraction, PCR amplification, and direct sequencing of the COL8A1 a

107 DNA extraction, PCR amplification, and direct sequencing of the VSX1 gen

108 abrication of microchips for DNA extraction, PCR amplification, and DNA fragment separation, includin

109 omplete assay, including sample preparation, PCR amplification, and electrochemical detection of Camp

110 oratory steps, DNA quantitation and pooling, PCR amplification, and electrophoresis, accounted for 23

111 tandard Sanger sequencing, genotype-specific PCR amplification, and non-HCV-specific Illumina RNA seq

112 g to errors in sampling, random mutations in PCR amplification, and probably mostly variations in rea

113 xtraction procedures, reverse transcription, PCR amplification, and real-time detection at target con

114 rimers were used for nonspecific (universal) PCR amplification, and serotype-specific probes coupled

115 rom bacterial ribosomes without the need for PCR amplification, and single DNA strands produced from

116 mber of templates are used in initiating the PCR amplification, and this can lead to unrecognized seq

117 ortant issues such as adapter ligation bias, PCR amplification artefacts or to include internal contr

118 ed in 50% examined transgenic cotton through PCR amplification assay and sequencing analyses.

119 CR (smPCR) amplification would eliminate the PCR amplification bias because competition between templ

120 w per-base sequencing quality at the 3' end, PCR amplification bias, and bisulfite conversion rates.

121 ng and detect cross-sample contamination and PCR amplification bias.

122 /deflation of DNA variant proportions due to PCR amplification biases and sequencing errors.

123 es of noise owing to incomplete RNA capture, PCR amplification biases and/or batch effects specific t

124 ved from metabarcoding due to taxon specific PCR amplification biases.

125 After the subsequent bisulfite treatment and PCR amplification, both cytosine and 5-caC (derived from

126 However, PCR amplification can introduce significant quantitative

127 RT-PCR amplification, cloning, and sequencing of the monocl

128 ed from small water samples using wide-range PCR amplification combined with high-throughput sequenci

129 Real-time RT-PCR amplification confirmed the presence of specific gen

130 Fluorescent output of multiplexed PCR amplification could also be imaged with the cell pho

131 However, in vitro recombination during PCR amplification could not be excluded.

132 PCR amplification coupled with electrospray ionization m

133 s and analyzes the samples using multiplexed PCR amplification coupled with microsphere array detecti

134 g multiplexed reverse transcription-PCR (mRT-PCR) amplification coupled with a microsphere hybridizat

135 through Poisson statistics, showed real-time PCR amplification curves with a cycle threshold of appro

136 e developed probes that, in combination with PCR amplification, detect low concentrations of variant

137 tudy, the use of the Endo IV assay as a post-PCR amplification detection system is demonstrated.

138 After RT-PCR amplification, DNAs amplified from bunyaviruses of i

139 rary generation relies on multiple rounds of PCR amplification, during which the accumulation of erro

140 despite poor and non-uniform extraction and PCR amplification efficiencies.

141 lfite-modified genomic DNA to a second-round PCR amplification employing GC-tagged primers.

142 After PCR amplification, end-point detection is achieved using

143 We also examined two strategies for PCR amplification-flanking PCR, which uses primers that

144 enables this information to be recovered by PCR amplification followed by DNA sequencing.

145 ducts), which uses target-enriched multiplex PCR amplification followed by liquid array identificatio

146 e-genome sequencing (WGS) strategy, based on PCR amplification followed by next-generation sequencing

147 th; this is presumably caused by inefficient PCR amplification for long fragments.

148 In addition, PCR amplification formulations were optimized to tolerat

149 After limited PCR amplification, fragments are sequenced using a high-

150 paper we report on a bisulfite treatment and PCR amplification-free method for sensitive and selectiv

151 used by A. terreus and A. niger, culture and PCR amplification from BAL fluid yielded similar sensiti

152 RT-PCR amplification from human hippocampal mRNA confirmed

153 dge that included both sample processing and PCR amplification functions was loaded with all reagents

154 ELP data analysis is complicated not only by PCR amplification heterogeneity but also by a complex an

155 and instrumentation to accomplish universal PCR amplification, High Resolution Melting (HRM), and ma

156 es that underpin genetic analysis, including PCR amplification, hybridization, whole-genome, target-e

157 here longer fragments are more available for PCR amplification in air dried material compared to alco

158 DNA extraction or polymerase chain reaction (PCR) amplification in just 75 min.

159 chastic capture of low input RNA rather than PCR amplification influences the biased quantitation of

160 PCR amplification introduces redundant reads in the sequ

161 PCR amplification is an important step in the preparatio

162 y characterize DNA variant proportions using PCR amplification is key to many genetic studies, includ

163 sequence polymorphism in Pacific oysters, Q-PCR amplification is sub-optimal in some individuals bec

164 A major influence on the PCR amplification is the size of the restriction fragmen

165 rectly extracted from lymphocytes (bypassing PCR amplification), is reported.

166 ng sample-specific tags in the primers prior PCR amplification, it is possible to multiplex hundreds

167 pproach with those of more-conventional bulk PCR amplification methods performed on the same patient

168 solution despite sequence-dependent bias and PCR-amplification noise, and is analogous to digital PCR

169 PCR amplification of 16S rRNA genes with universal prime

170 Applied to the PCR amplification of 47,000-60,000-year-old cave bear DN

171 as indicated by the subsequent chip-based IR-PCR amplification of a 211-bp fragment of the B. anthrac

172 irst method, the isolates were studied using PCR amplification of a fragment of the C. parapsilosis s

173 = 3), and the purified DNA was suitable for PCR amplification of a fragment of the gelsolin gene.

174 Although assays utilizing PCR amplification of bisulfite-converted DNA are widely

175 conditions and increased sensitivity in the PCR amplification of bisulfite-treated DNA.

176 template for DNA polymerization, that allows PCR amplification of boronic acid-labeled DNA.

177 In addition, simultaneous real-time PCR amplification of both multiple different samples and

178 quence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistan

179 the Dpo4-like enzymes ideally suited for the PCR amplification of damaged DNA samples.

180 Here, we examine the PCR amplification of DNA containing one or more d5SICS-d

181 tified as Enterococcus hirae on the basis of PCR amplification of DNA extracted from the formalin-fix

182 Typically, PCR amplification of DNA from each panel cell line is fo

183 PCR amplification of DNA is a key preliminary step in ma

184 Finally, we document PCR amplification of DNA with two dimeric nucleotides, a

185 ase has served as the stalwart enzyme in the PCR amplification of DNA.

186 hanisms involved in chimera formation during PCR amplification of environmentally derived DNA.

187 to those of natural isolates sequenced by RT-PCR amplification of field samples.

188 perature and can be used as the template for PCR amplification of fragments of at least 8 kb.

189 Brain inflammation was measured by TaqMan RT-PCR amplification of genes known to be up-regulated in r

190 PCR amplification of genomic DNA and sequencing of the g

191 Here, we describe a method for PCR amplification of lesion-containing DNA in which the

192 The C(T) values for PCR amplification of lysed samples using primers specifi

193 , in the inoculated models, was confirmed by PCR amplification of M13 minisatellite.

194 In the DNA-based method, PCR amplification of mitochondrial D loop gene using spe

195 The PCR amplification of oligonucleotides enables the evolut

196 g sequence is labeled with a unique OMT, and PCR amplification of OMTs is performed after removing no

197 ns on streptavidin-coated magnetic beads and PCR amplification of single-cell Hi-C libraries.

198 fluorescence-labeled oligonucleotide probes, PCR amplification of species-specific gene sequences, an

199 where conventional thermocycling allowed for PCR amplification of specific DNA target sequences.

200 Traditional STR analyses were based on the PCR amplification of STR loci followed by gel electropho

201 ps: pooled growth, isolation of genomic DNA, PCR amplification of the barcodes, array hybridization a

202 with sodium bisulphite, usually followed by PCR amplification of the chosen target region.

203 on through enzymatic ligation; and (iii) qRT-PCR amplification of the circularized PLP after removal

204 samples by Ms-SNuPE in less than 5 h, after PCR amplification of the desired target sequence and pre

205 PCR amplification of the GAPDH gene was demonstrated at

206 ently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (

207 liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes.

208 Genotyping was performed after PCR amplification of the region encompassing the polymor

209 ; ligation of an adapter to the genomic DNA; PCR amplification of the regions flanking the T-DNA inse

210 erse transcription of the associated RNA and PCR amplification of the resultant cDNA with gene-specif

211 be distinguished from those arising through PCR amplification of the same molecule.

212 ligation of an adapter to the genomic DNA; a PCR amplification of the T-DNA/genomic DNA junction with

213 results were compared to those of ELISA and PCR amplification of the toxin genes.

214 ted from the plaque samples and subjected to PCR amplification of the V1-V3 region of the 16S rDNA.

215 cell RNA, enabling reverse transcription and PCR amplification of these sequences.

216 PCR amplification of three unrelated genes resulted in c

217 shearing of genomic DNA followed by specific PCR amplification of transposon-containing fragments and

218 ird CDR of the H chain intervals obtained by PCR amplification of V(H) domain DNA from DEX-specific p

219 tive immune receptor repertoires depend upon PCR amplification of VDJ rearrangements followed by long

220 the experimental conditions are suitable for PCR amplification of VEGF-A165b mRNA for both q-PCR and

221 ese determinations were made by either using PCR amplification of viral transcripts in bulk cell popu

222 man GLTP gene, in silico EST evaluations, RT-PCR amplifications of GLTP transcript(s), and methylatio

223 e progress of DNA polymerase chain reaction (PCR) amplification of a minor component of a DNA extract

224 opic observation, polymerase chain reaction (PCR) amplification of blood samples, and serological tes

225 id was extracted, polymerase chain reaction (PCR) amplification of desmosome-encoding genes was perfo

226 transcription polymerase chain reaction (qRT-PCR) amplification of miRNA extracted directly from 500

227 lfite followed by polymerase chain reaction (PCR) amplification of specific regions of interest that,

228 was performed by polymerase chain reaction (PCR) amplification of tail genomic DNA and resequencing.

229 s accomplished by polymerase chain reaction (PCR) amplification of the 16S rRNA and hsp65 genes.

230 ncing followed by polymerase chain reaction (PCR) amplification of the cyclin A promoter (-267/37) sh

231 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (>83%) DNA target

232 However, PCR-amplification of GC-rich templates is often hampered

233 s validated using polymerase chain reaction (PCR) amplification, of which 167 primers gave expected P

234 proach includes the design and validation of PCR amplification on bisulfite-treated DNA and pyroseque

235 revent successful downstream DNA extraction, PCR amplification or sequencing.

236 single DBS, 2 DBS samples with insufficient PCR amplification, or known genetic risk of immunodefici

237 PCR amplification over GC-rich and/or long repetitive se

238 icient ligation to target DNA and subsequent PCR amplification primed by the T oligo alone.

239 s an algorithm for reverse transcription and PCR amplification primer design using all of the publica

240 was generated by polymerase chain reaction (PCR) amplification, primer-walking sequencing and fragme

241 indered by the need of standard or real-time PCR amplification procedures.

242 detection without reverse transcription and PCR amplification processes.

243 ORF14, ORF16, and Ori regions of pMR during PCR amplification produced a temperature-sensitive plasm

244 PCR/ESI-MS) and base composition analysis of PCR amplification products can quickly and accurately id

245 plied a genomewide adapter-mediated emulsion PCR amplification protocol for ancient mammalian samples

246 ures as well as other microarray designs and PCR amplification protocols.

247 eet the criteria for success with one of two PCR amplification protocols.

248 lated positively with the higher sample-wide PCR amplification rate, lower template-to-template PCR b

249 Allele-specific polymerase chain reaction (PCR) (amplification-refractory mutation system, ARMS) is

250 in real-time, without any cDNA synthesis or PCR amplification requirements.

251 ive restriction endonucleases followed by AP-PCR amplification resulted in the detection of unknown u

252 The PCR amplification results of extracted DNA from the thre

253 udies of the intraepithelial lymphocytes and PCR amplification revealed surface expression of CD3 and

254 Using straightforward PCR amplifications, Sanger sequencing and blast analysis

255 During polymerase chain reaction (PCR) amplification, selective nucleotides included at th

256 was purified, and polymerase chain reaction (PCR) amplification showed similar threshold cycle values

257 icroplate format without the need for sample PCR amplification, showing the potential suitability of

258 s may be caused in part by a bias during the PCR amplification step that discriminates against longer

259 ompleting the assay are included in a single PCR amplification step, assay costs, preparation time, a

260 abeling, and replicate clones created during PCR amplification steps can be identified and assigned t

261 rmance in terms of DNA purity and yield, and PCR amplification success as measured by using three dif

262 tes in a large, downloaded DNA database, and PCR amplification supported the general organization of

263 Most PCR amplification systems provided similar detection thr

264 ing rolling circle amplification with random PCR amplification tagging (RCA-RA-PCR), high-throughput

265 PCR amplification targeting the 16S rRNA gene was used t

266 Using PCR amplification techniques, a genomic clone correspond

267 After bulk PCR amplification, the droplets are lysed and the beads

268 above the microfluidic chip, which expedited PCR amplification to 18.8 min for a 30-cycle, three-temp

269 s verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obt

270 shotgun sequencing (RNA-Seq) method without PCR amplification to cultured viral stocks and patient p

271 ease IV, followed by primer extension and/or PCR amplification to detect the endonuclease-generated s

272 kbone that is sufficiently biocompatible for PCR amplification to generate a cDNA library for RNAseq.

273 Recent Patch-Seq studies utilizes PCR amplification to increase amount of nucleic acid mat

274 NA from low-abundance RNA and the subsequent PCR amplification under conditions which provide amplico

275 subjected to insert-specific primer-mediated PCR amplification using a high-fidelity DNA Pfu polymera

276 PCR amplification using COnsensus DEgenerate Hybrid Olig

277 P and 2-thioTTP were optimal to best support PCR amplification using thermostable polymerases of a si

278 When PCR amplification was combined with DNA extraction, 50%

279 PCR amplification was done for nine highly polymorphic s

280 Real-time PCR analysis showed that the PCR amplification was not impacted by time for any swab

281 PCR amplification was performed targeting 16S rRNA/tRNA(

282 A method using adapter-ligation and PCR amplification was successfully applied to visualize

283 al limit of detection (LOD) of the Genedrive PCR amplification was tested with genomic DNA; the perfo

284 TaqMan real-time PCR amplification was used to detect the presence of H.

285 and quantity for polymerase chain reaction (PCR) amplification was recovered from the MIL extraction

286 e modified, since cDNA yields obtained by RT-PCR amplification were severely reduced and no infectiou

287 PCR amplifications were performed for T-Ag sequences, an

288 transcription and polymerase chain reaction (PCR) amplification were no longer needed.

289 Previous methods have relied on PCR amplification, which is problematic due to primer de

290 argeted resequencing, including microfluidic PCR amplification, which may be enhanced by multiplex PC

291 Following PCR amplification with indexing primers, the subnucleoso

292 PCR amplification with primers not reaching a high speci

293 Bisulfite-modified DNA is subjected to PCR amplification with primers that would differentiate

294 tion, followed by reverse transcription, and PCR amplification with real-time fluorescence detection

295 system for picoliter droplet generation and PCR amplification with real-time fluorescence detection

296 Using PCR amplification with targeted and degenerate primers f

297 A gene amplicon sequencing based on two-step PCR amplifications with tagged primers for plates, rows,

298 verse transcription and 30 cycles of PCR (RT-PCR) amplification with capillary electrophoresis (CE) s

299 ing for reverse transcription and subsequent PCR amplification without droplet motion.

300 of substrate pairs, in vitro selection, and PCR amplification, yet does not require reaction conditi