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1 ICU admission were tested using a multiplex PCR assay.
2 g a previously validated EV-D68-specific rRT-PCR assay.
3 , a dual-target total nucleic acid real-time PCR assay.
4 th 1484 (12%) of these cases confirmed on RT-PCR assay.
5 rcial stool antigen assays, and a commercial PCR assay.
6 h those demonstrated by the duplex real-time PCR assay.
7 ty and technical time may be possible with a PCR assay.
8 as measured by an ultrasensitive single-copy PCR assay.
9 A fraction was quantified by quantitative RT-PCR assay.
10 and tested for SARS-CoV-2 using a real-time PCR assay.
11 la invasion antigen, IpaH3, into a multiplex PCR assay.
12 NAs (miRNA), termed S-Poly(T) Plus real-time PCR assay.
13 edding and VL using a real-time quantitative PCR assay.
14 and 65 (47%) were positive by the real-time PCR assay.
15 genes were assayed by quantitative real-time PCR assay.
16 amples and obtain consistent results with RT-PCR assay.
17 positive visits were measured by a real-time PCR assay.
18 A-Seq were corroborated through real-time RT-PCR assay.
19 ies, showing comparable results to real-time PCR assay.
20 the gold standard of tear collection for RT-PCR assay.
21 to reduced inhibition with the one-step ddRT-PCR assay.
22 ults alone, and both also tested positive by PCR assay.
23 t were corroborated by culture or a clinical PCR assay.
24 nosinic-polycytidylic acid using a sensitive PCR assay.
25 as 7.5 h compared to 40.3 h for the standard PCR assay.
26 ts between established LAMP and conventional PCR assays.
27 the reliance on more expensive and laborious PCR assays.
28 en, the Netherlands) and published multiplex PCR assays.
29 ith mRNA detection based principally upon RT-PCR assays.
30 ssion in single samples using repetitive qRT-PCR assays.
31 as revealed by chromatin immunoprecipitation-PCR assays.
32 NA was isolated and used as template for the PCR assays.
33 e G and P genotyped using nested VP4 and VP7 PCR assays.
34 transcription factors was analyzed by using PCR assays.
35 ound to be more sensitive than the real-time PCR assays.
36 f acanthamoebae with four separate real-time PCR assays.
37 n compared to species-specific SARS-CoV-2 RT-PCR assays.
38 We compared the results obtained with ELISA/PCR assays.
39 vity and specificity compared to centralized PCR assays.
40 L, making it an attractive alternative to RT-PCR assays.
41 pared to 10 to 10(4) copies per reaction for PCR assays.
42 including HRV, using quantitative real-time PCR assays.
43 itative real-time polymerase chain reaction (PCR) assay.
44 triplex real-time polymerase chain reaction (PCR) assay.
45 croscopy and by a polymerase chain reaction (PCR) assay.
46 -transcriptase-polymerase-chain-reaction (RT-PCR) assay.
47 in West Africa by polymerase-chain-reaction (PCR) assay.
48 transcription-polymerase chain reaction (RT-PCR) assay.
49 transcriptase-polymerase-chain-reaction (rRT-PCR) assay.
50 -transcriptase-polymerase-chain-reaction (RT-PCR) assay.
51 and mycobacterial polymerase chain reaction (PCR) assays.
52 donors whose samples tested positive on the PCR assay 2 to 7 months after the implicated donation.
53 by culture and negative for norovirus by RT-PCR assay; 4 specimens were positive for rotavirus by en
55 cted from cultured isolates and biopsies for PCR assay after which samples were investigated using st
56 nsitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish
57 ea lion populations and results suggest that PCR assays alone may grossly underestimate ZcAV exposure
59 ted with both ELISAs and the three real-time PCR assays although mustard was not indicated on the foo
62 step real-time reverse transcription PCR (RT-PCR) assays; an accurate sample-to-answer RT-PCR test wo
63 ng using another commercially available HAdV PCR assay and a molecular typing assay for species ident
64 uenza infection was confirmed by means of RT-PCR assay and culture of nasopharyngeal swabs obtained f
65 total of 905 patients tested by real-time RT-PCR assay and next-generation sequencing RT-PCR, 419 (46
66 as demonstrated by using WHV strain-specific PCR assays and (i) finding WHVNY relaxed circular DNA in
68 pective samples were resolved with alternate PCR assays and bidirectional sequencing of amplicons.
69 na in situ hybridisation (ISH), quantitative PCR assays and biomarkers of productive and transforming
70 mized set of oligonucleotide primers for qRT-PCR assays and cloned cDNA plasmids corresponding to the
71 re and broad-range and E. rostratum-specific PCR assays and confirmed the presence of fungal DNA in 2
73 ime points was confirmed by variant-specific PCR assays and sequence analysis of single-colony cloned
74 -transcription polymerase chain reaction (RT-PCR) assay and a clinical reference standard established
75 , 41 (29%) were positive by the conventional PCR assay, and 65 (47%) were positive by the real-time P
77 ession was analyzed by using RNA-sequencing, PCR assays, and immunostaining in patients with EoE; cyt
78 es by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/31
79 ole in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool
81 B/RIF Ultra (Xpert Ultra), a fully automated PCR assay, as the initial tuberculous meningitis (TBM) d
85 yme-linked immunosorbent assay (ELISA) and a PCR assay based on amplification of the pan-Aspergillus
89 ignal produced from probe-based RT-RPA or RT-PCR assays can be monitored using LEDs and a smartphone
90 C, Salt Lake City, UT), a highly multiplexed PCR assay, can identify 24 etiologic agents of sepsis (8
91 he 24 human chromosomes that allow for rapid PCR assays capable of capturing the genomic landscape of
92 We compared an FDA-cleared rapid (<20 min) PCR assay (Cobas Liat; Roche Diagnostics) to our routine
93 tica and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity and specifi
96 was assessed with a multiamplicon, real-time PCR assay designed to analyze locations that are regular
97 otypes IA, IB, and IIIA and a singleplex rRT-PCR assay designed to detect all HAV genotypes infecting
99 ng the 201 specimens tested, the CDC Flu A/B PCR assay detected Flu A/B virus in 107 samples (Flu A v
104 mposite reference method (CRM; two validated PCR assays followed by bidirectional sequencing of ampli
105 isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temper
107 erium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed 95% (38/4
110 y (Luminex, Austin, TX) is a fully automated PCR assay for direct detection of GAS in throat swab spe
111 vailable genomic sequence data to evaluate a PCR assay for distinguishing Campylobacter species.
112 nducible Cas9 (ciCas9) and a droplet digital PCR assay for double-strand breaks (DSB-ddPCR) to invest
113 City, QC, Canada) is an automated real-time PCR assay for GAS detection from throat swab specimens w
115 ice junction, we developed a quantitative RT-PCR assay for K-Ras4A and K-Ras4B message capable of mea
116 ssay was more appropriate than the real-time PCR assay for monitoring the response to treatment.
117 re, we describe a SYBR green-based real-time PCR assay for Plasmodium species identification from who
118 sed-tube Linear-After-The-Exponential (LATE)-PCR assay for pyrazinamide susceptibility in Mycobacteri
119 and extensively validated a novel multiplex PCR assay for ST1193 and its characteristic fimH64 (type
120 t and evaluation of a novel TaqMan real-time PCR assay for the detection and identification of Ricket
121 rformance of a simple, inexpensive real-time PCR assay for the detection of 13 carcinogenic HPV types
123 lopment and validation of a TaqMan real-time PCR assay for the detection of E. rostratum in body flui
124 the present study was to develop a real-time PCR assay for the identification and quantification of f
126 of an in-house developed C. auris real-time PCR assay for the rapid screening of patient and environ
129 oV-2 and Hologic Panther Fusion real-time RT-PCR assays for detection of viral RNA in stool specimens
130 and tested by clinically validated real-time PCR assays for Ehrlichia spp., Anaplasma phagocytophilum
131 dized approaches (Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxi
132 the case of a DNA standard used in real-time PCR assays for human erythropoietin gene, cDNA or transc
133 ted at baseline and at 6 months by real-time PCR assays for MG detection in urine samples, oro-pharyn
134 ensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses, including an assay f
135 e of the RP panel in comparison to real-time PCR assays for the detection of respiratory pathogens.
138 by a multiplexed polymerase chain reaction (PCR) assay for 22 bacterial, viral, and protozoan gastro
140 multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection but 10-fold
141 ed a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (E
142 time, reverse transcription-PCR (triplex rRT-PCR) assay for the identification and discrimination of
143 ltiplex real-time polymerase-chain reaction (PCR) assays for 26 respiratory bacteria and viruses.
144 ies and real-time polymerase-chain-reaction (PCR) assays for B. microti DNA on blood-donation samples
145 -transcriptase-polymerase-chain-reaction (RT-PCR) assays for ZIKV in blood, cerebrospinal fluid, and
146 ormed using a laboratory-developed real-time PCR assay; for the postimplementation group, CSF samples
148 tative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prev
149 aph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) f
153 ial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization fr
154 of the BD Max enteric bacterial panel (EBP) PCR assay in comparison to culture for the detection of
155 (kappa of >/=0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic samples.
156 lico approaches for designing and validating PCR assays in the clinical microbiology laboratory.
158 -transcriptase-polymerase-chain-reaction (RT-PCR) assay in urine or blood in an enhanced arboviral cl
162 s detection in the upper airway by multiplex PCR assay is common in critically ill hematology patient
163 results also demonstrate that the real-time PCR assay is extremely susceptible to contamination and
164 ssibility for some assays, in well-optimized PCR assays it can reduce the number of amplifiable targe
168 ffectiveness, and the simplicity of the ARMS-PCR assay make it a suitable tool to monitor HIVDR patte
170 ssay results it has been concluded that SDRT-PCR assay might be an efficient tool for the verificatio
174 proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstein-Barr virus
177 loped and validated a TaqMan-based real-time PCR assay on the BD Max platform targeting ribosomal DNA
178 fficiency of the SAF microlens array, the SP-PCR assay on the LOC platform demonstrated a high sensit
179 y assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the durati
180 report results of measurements from multiple PCR assays, on four human genomic DNAs treated with four
181 In contrast to conventional quantitative PCR assays or decoy cell counts, quantitative urinary PV
182 We evaluated a genus- and group-specific PCR assay panel using 284 prosthetic knee synovial fluid
183 Henceforth, this DNAzyme-based fiber optic PCR assay provides a universally applicable, real-time s
184 action of RNA, the multiplex quantitative RT-PCR assay provides rapid diagnosis for the differential
185 wed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the clinical
186 age, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice for the
190 Global expression analysis and real-time PCR assays revealed that RhERF1 and RhERF4 modulate the
191 lyzed using two EV-D68-specific real-time RT-PCR assays, Sanger sequencing and metatranscriptomic nex
194 , chromatin immunoprecipitation-quantitative PCR assay showed enrichment of p63 on Sun1, Syne3, and P
196 ) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focus Diagnost
197 otal) and three in-house developed real-time PCR assays (singleplex assay for white mustard, singlepl
199 etection of M. pneumoniae The ELITe InGenius PCR assay successfully detected 31 distinct M. pneumonia
200 We developed a novel reverse transcription-PCR assay targeting HHV-6B U38, which identified U38 mRN
201 th those of a laboratory-developed real-time PCR assay targeting repMp1 for detection of M. pneumonia
205 ted a laboratory-developed real-time PCR (LD-PCR) assay targeting stx1, stx2, and rfbEO157 with 2,386
206 eport a tetraplex polymerase chain reaction (PCR) assay targeting two different DNA regions in beef (
208 n using real-time polymerase chain reaction (PCR) assays targeting the opa gene and porA pseudogene.
209 with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes.
210 evaluated the performance of a new real-time PCR assay - targeting SP2020 (putative transcriptional r
211 he development and evaluation of a real-time PCR assay that can be performed directly on crude DNA ex
212 a hydrolysis probe-based tetraplex real-time PCR assay that can simultaneously detect and differentia
213 sequence, we developed a multiplex touchdown PCR assay that in a single reaction can confirm the spec
214 We demonstrate a simple droplet digital PCR assay that quickly quantitates a range of indel muta
215 data to evaluate a duplex real-time PCR (RT-PCR) assay that targets mapA and ceuE for the detection
216 volving real-time polymerase chain reaction (PCR) assays that facilitate direct analysis of a single
217 llele-specific polymerase chain reaction (AS-PCR) assays that we developed for Bruton tyrosine kinase
218 were positive by an alternative real-time RT-PCR assay (the Trombley assay); three (17%) of 18 sample
219 Compared to fungal culture and the previous PCR assays, this real-time PCR assay was more sensitive.
225 ing the concentration obtained with the deer PCR assay to that obtained with a reference system which
227 The ability of PfLDH- or PfIDEh-based immuno-PCR assays to detect <1 parasite/microL suggests that im
228 ed 783 subjects with RDT-/PCR+ results using PCR assays to detect and confirm deletions of the pfhrp2
230 y-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Can
231 f South Africa were used in duplex real-time PCR assays to simultaneously detect A. marginale and A.
232 loped a sensitive polymerase chain reaction (PCR) assay to quantify the HBV RNA load in a supernatant
233 cing and novel reverse transcription-PCR (RT-PCR) assays to distinguish all RNA species in the KSHV l
235 irus (EBV) load testing in four quantitative PCR assays, treating digital PCR as a reference assay.
239 herefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS system pr
240 species-specific polymerase chain reaction (PCR) assay using conserved regions of mitochondrial DNA
241 , sensitive, specific molecular-beacon-based PCR assay, using primers against pfoB gene of Trichomona
242 with those of laboratory-developed real-time PCR assays, using a variety of previously collected clin
244 ether, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and c
248 c species ofRickettsia The nested 23S-5S IGS PCR assay was coupled with reverse line blot hybridizati
250 lification refractory mutation system (ARMS)-PCR assay was developed and used to investigate the most
255 utatum in complex olive matrices a real-time PCR assay was developed, using olive drupe and oil sampl
263 ea, positivity of N. gonorrhoeae DNA on both PCR assays was present at days 7 or 14 in 13% (95% confi
265 At baseline, a polymerase-chain-reaction (PCR) assay was performed on blood samples obtained from
266 and sequential chromatin immunoprecipitation-PCR assay, we found that ZNF322A could form a complex wi
267 Here, using a very sensitive multiplex RT-PCR assay, we screened 1,645 formalin-fixed paraffin-emb
268 n assays, Rho activation and quantitative RT-PCR assays, we investigated the mechanism by which CS-GR
269 cer protection, sequencing, and quantitative PCR assays, we show here that the bacterial 3'-5' exonuc
277 R group" (the results of serial serum GM and PCR assays were provided to treating physicians) and "GM
278 amplification (RCA) and droplet digital (dd)PCR assays were used to quantify residual intrahepatic c
280 transcription polymerase chain reaction (RT-PCR) assays were compared together and with the final di
282 We have designed a novel triplex real-time PCR assay which simultaneously amplifies the MT-ND4 gene
283 nformation of ITS2, we developed a Real-Time PCR assay which successfully identified herbal material
284 ency, an advantage of the IPDA over standard PCR assays which generate false-negative results in such
286 lidated the first direct multiplex real-time PCR assay with melt curve analysis for the identificatio
287 we compared a laboratory-developed multiplex PCR assay with primers and probes specific for each grou
288 real-time quantitative reverse transcriptase PCR assay with single-copy sensitivity targeting the int
289 The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate po
290 of four quantitative Epstein-Bar virus (EBV) PCR assays with the WHO and ABI standards as examples.
291 -transcriptase-polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP an
293 CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMerieux] and
294 ntrol and Prevention SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 10
295 and Panther Fusion, and their associated RT-PCR assays, with a collection of 389 nasopharyngeal spec
296 tified in silico for all deployed diagnostic PCR assays, with the exception of the Cepheid GeneXpert
297 en tested by different real-time and digital PCR assays, with various magnitudes of bias compared to
298 -transcriptase-polymerase-chain-reaction (RT-PCR) assay, with consistent findings on electron microsc