ライフサイエンスコーパス: PCR primer

1 placed 6-10 nt away from the 5'-end of each PCR primer.

2 ined BSMV RNA nucleotides not present on the PCR primer.

3 ion junction for the addition of a real-time PCR primer.

4 ze corresponding allele-specific tags on the PCR primers.

5 ing the physico-chemical constraints of good PCR primers.

6 inct alleles with one pair of microsatellite PCR primers.

7 nscribed spacer 2 (ITS2) Malassezia-specific PCR primers.

8 -specific probes for expression analyses and PCR primers.

9 des to tags attached to the allele- specific PCR primers.

10 uclease restriction sites into the 5'-end of PCR primers.

11 ing genus- and species-specific helicobacter PCR primers.

12 m this enzyme were used to design degenerate PCR primers.

13 binations of oligo(dT) and various arbitrary PCR primers.

14 tellite loci amplified using published human PCR primers.

15 genes were amplified by means of broad-range PCR primers.

16 rate gene identification with relatively few PCR primers.

17 veloped that facilitates the design of these PCR primers.

18 The modified oligonucleotides were tested as PCR primers.

19 mer design tool that designs target-specific PCR primers.

20 limited serotype coverage requiring multiple PCR primers.

21 Ralstonia, for selecting loci and designing PCR primers.

22 mologies to targeted regions incorporated on PCR primers.

23 hamsters by the use of Helicobacter-specific PCR primers.

24 icient as a mean of identifying acceptable Q-PCR primers.

25 the human genome, which may prove useful as PCR primers.

26 evaluation of the polymerase chain reaction (PCR) primer.

27 rrently available polymerase chain reaction (PCR) primers.

28 ans should not rely on results using current PCR primers alone to decide the H. pylori status of an i

29 nts a set of recommended forward and reverse PCR primers, along with a sequencing primer, which is op

30 The standard PCR primers amplify approximately 450 bp of VP1 for most

31 unique oligo problem and has applications in PCR primer and microarray probe designs, and library scr

32 proving nucleic acid recovery, designing qRT-PCR primers and a probe for a highly conserved region of

33 eviously developed methods using fluorescent PCR primers and amplicon sizing on a Sanger-style sequen

34 verified as the WTD agent by using specific PCR primers and by DNA sequencing.

35 (451-846)) was subcloned using site-specific PCR primers and expressed as six smaller fragments: OmpB

36 ults may be used in the design of degenerate PCR primers and for degenerate microarray probes.

37 The Oligo data table contains PCR primers and hybridization probes used for detection

38 due to potential applications as degenerate PCR primers and microarray probes.

39 The selected PCR primers and probe did not generate fluorescent signa

40 A perfect match with the PCR primers and probe was seen in 81 sequences.

41 ithm was used to design degenerate real-time PCR primers and probes based upon a large number (n = 34

42 PCR primers and probes were designed specifically for de

43 PCR primers and probes were designed to identify gene se

44 alleles were identified with allele-specific PCR primers and probes.

45 ftware tool for designing sequence-selective PCR primers and probes.

46 use of standardized reference materials and PCR primers and probes.

47 the bacterial 16S rRNA gene, using universal PCR primers and querying the identity of specific single

48 een extremely slow, and key reagents such as PCR primers and RNA extraction kits are at critical shor

49 ting of mirror-image L-DNA surrogates of the PCR primers and targets.

50 The TRUGENE assay using prototype 1.5 RT-PCR primers and the ViroSeq assay were both successful f

51 ted by a core sequence composed of universal PCR primers, and a qRT-PCR reporter binding site.

52 These PCR primers annealed to E. platys 16S rDNA in blood samp

53 ariations in DNA fine structure that prevent PCR primer annealing may occur in this region and make S

54 tion of this technique where mismatches in a PCR primer are used to create a polymorphism based on th

55 Historically, PCR primers are designed so that each primer occurs uniq

56 PCR primers are designed to maximize amplification succe

57 PCR primers are located in the unique regions bordering

58 n to occur in an allele-specific manner, the PCR primers are manipulated such that an extra mismatch

59 uencing, a novel procedure in which multiple PCR primers are used in a single sequencing reaction, is

60 In our system, fluorescently labeled PCR primers are used to produce PCR amplification produc

61 p PCR', involves addition of a radiolabelled PCR primer at the final cycle.

62 Traditional asymmetric PCR uses conventional PCR primers at unequal concentrations to generate single

63 By designing degenerate PCR primers based on amino acid sequences that are highl

64 PCR primers based on consensus gene sequences conserved

65 Degenerate PCR primers based on highly conserved areas of other fun

66 PCR primers based on sequence differences between the kn

67 -kDa proteins were sequenced, and degenerate PCR primers based on these sequences were used to amplif

68 PCR primers based on this sequence were used to screen a

69 d at each terminus and an interposed generic PCR primer binding region.

70 ons enables identification of conserved core PCR primer binding sites flanking polymorphic haplotypes

71 ossible A/B recombinants exhibiting DWV-B at PCR primer binding sites, may be a major cause of elevat

72 This region was amplified using PCR primers binding to all known MV genotypes.

73 These target DNA regions and PCR primers can then be utilized to type bacterial isola

74 within each internal gene, and gene-specific PCR primers capable of amplifying all three virus subtyp

75 the design of reverse transcription-PCR (RT-PCR) primers capable of detecting these markers.

76 fied by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequenci

77 we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosi

78 ted from doubly infected plants by using one PCR primer corresponding to the 5' termini of the BSMV R

79 by ovarian carcinoma, we designed degenerate PCR primers corresponding to the conserved regions of th

80 PCR primer design aims to amplify as many different 16S

81 uch web-based or standard-alone programs for PCR primer design are available but vary in quality and

82 Using degenerate PCR primer design based on published and aligned MAP kin

83 Integrated PCR primer design is also provided for each signature.

84 t step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality

85 The high-throughput PCR primer design pipeline has been very successful in p

86 e developed a fully integrated computational PCR primer design pipeline that plays a key role in our

87 ides (or simply, oligos) that can be used in PCR primer design, microarray experiments and genomic li

88 ons such as restriction digestion, ligation, PCR, primer design, Gibson assembly and homologous recom

89 ation, we developed species-specific PCR (SS-PCR) primers, designated SM1 and SM4, directed to the 23

90 Here, a PCR primer designed to generate a PCR amplicon tagged wi

91 PCR primers designed around exon 3 of the PRH1 locus gav

92 Utilizing PCR primers designed by Hill et al. to amplify across th

93 A typing scheme using PCR primers designed for four of these locations was app

94 r 348 bp of the Dhc-Yh3 gene, and degenerate PCR primers designed from comparisons of the sea urchin

95 PCR primers designed from sequences in the genomic datab

96 PCR primers designed to amplify an ETV6-CDX2 fusion iden

97 NA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and rando

98 Using PCR primers designed to specifically target archaeal amo

99 Multiple PCR primers designed to this region amplified products t

100 f families not present in the database and a PCR primer designer that implements a new strategy for g

101 ntron and exon position data critical to qRT-PCR primer development, and supplies these sites as iden

102 ChIP using a specific anti-Brd4 antibody and PCR primers directed against the transcriptional start s

103 DNA was extracted and amplified by using PCR primers directed toward the RDR of the rpoB, katG, a

104 We describe a method for producing specific PCR primers directly from PCR product, bypassing the usu

105 ypical nos), and that previous nosZ-targeted PCR primers do not capture the atypical nosZ diversity.

106 o require bespoke species- or group-specific PCR primers due to extensive genetic diversity across th

107 arcode success rate, most likely due to poor PCR primer efficiency.

108 Internally labeled PCR primers eliminate EndoV cleavage at the 5' terminus,

109 Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis.

110 d alternative splicing variants (ASVs) using PCR, primer extension and matrix-assisted laser desorpti

111 the electrophoretic mobility shift assay, RT-PCR, primer extension, and beta-galactosidase assay resu

112 screening method, termed PPEM (PNA-directed PCR, primer extension, MALDI-TOF), that addresses these

113 Twenty multiplex PCR/primer extension reactions were set up and each samp

114 ism and implements Primer3 for the design of PCR primers flanking polymorphic microsatellite loci.

115 PCR primers flanking the microsatellite repeats were des

116 tem for the t(11;22) has been developed with PCR primers flanking the PATRRs of both chromosomes, to

117 PCR primers flanking the TGC repeat were used to amplify

118 a monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification.

119 SNPsFinder can generate PCR primers for all predicted SNP regions according to u

120 ly, we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered re

121 n serve as good candidates for selecting the PCR primers for experimental work for gene structure ver

122 l challenges which include: the designing of PCR primers for FFPE tumor tissue samples versus normal

123 Because the rates at which junction-testing PCR primers for genomic survey sequences (90-92%) amplif

124 lly as P. aeruginosa, all were positive with PCR primers for gyrB or oprI, 98% were positive with exo

125 eviously been used to develop genus-specific PCR primers for identification of enterococci.

126 lso isolated and used to generate probes and PCR primers for mapping the pccb locus in the mouse.

127 ased on Primer 3, is a program for designing PCR primers for methylation mapping.

128 he implementation of an algorithm to predict PCR primers for most known human and mouse genes.

129 ckage that automates the design of multiplex PCR primers for next-generation sequencing.

130 d using a rapid DNA extraction technique and PCR primers for sdrCDE, clfA, clfB, sspA, and spa.

131 Using degenerate PCR primers for the conserved metalloprotease and disint

132 Therefore, we designed and tested new PCR primers for the identification of E. equi.

133 ment of recA sequences enabled the design of PCR primers for the specific detection of each of the si

134 program for identifying 16S rRNA probes and PCR primers for use as phylogenetic and ecological tools

135 Although reverse transcription-PCR (RT-PCR) primers for human NLV recognize NLV-BECs, the genet

136 e program has been written to design CODEHOP PCR primers from conserved blocks of amino acids within

137 n for designing conserved genomic PCR and RT-PCR primers from multigenome alignments targeting specif

138 g conserved sequences within the H19 gene as PCR primers, genomic DNA fragments were amplified from a

139 equence including polymerase chain reaction (PCR) primers, high-fidelity DNA amplification by PCR and

140 PNA probes were designed to compete with PCR primers hybridizing to the HH mutation site.

141 medium strength binding sites to a candidate PCR primer in an average of 34.5 minutes.

142 of LNA on the performance of sequencing and PCR primers in AT-rich regions, where short primers yiel

143 limited amplification using adapter-specific PCR primers in preparation for sequencing.

144 re used to test the utility of the novel PET-PCR primers in the diagnosis of clinical samples.

145 The mismatches in the PCR primers, in combination with the single-nucleotide c

146 rase was not required for ligation to the LM-PCR primer, indicating that the 3' DNA ends are extended

147 PCR primer information is provided for all array indels,

148 K-ras mutation status was determined by the PCR-primer introduced restriction with enrichment for mu

149 e-nucleotide changes by utilizing mismatched PCR primers is described.

150 The quality of PCR primers is further evaluated using melting temperatu

151 One of the PCR primers is modified by an oligonucleotide "tail" flu

152 PCR primers matched to normal and hypermutated sequences

153 d merging, barcode demultiplexing, 5' and 3' PCR primer matching, and duplicate reads collapsing.

154 ated in a diploid organism, a single pair of PCR primers may amplify as many as four distinct alleles

155 es present in any wheat cDNA sample to which PCR primers may then be designed.

156 For numerous experimental applications, PCR primers must be designed to efficiently amplify a se

157 In assays with PCR primers not included within that region, only four c

158 se DNA, acting therefore as an intracellular PCR primer of mdr1 mRNA, but not in cells exposed to sen

159 or the identification of transcript-specific PCR primers on a genomic scale that can be applied to re

160 highly degenerate, serine-protease-specific PCR primers on a midgut-specific cDNA library it was est

161 icans due to the use of intron-complementary PCR primers on genomic DNA samples.

162 his process frequently amounts to 'guessing' PCR primers on top of unreliable gene predictions and fr

163 Using universal bacterial PCR primers, one nearly full-length 16S rRNA gene could

164 ld also have been co-amplified with the same PCR primers; only the ligated C-probes were amplified.

165 The presence of a single universal PCR primer pair in solution accelerates amplification wi

166 vel sequence-based method employing a single PCR primer pair is cost-effective and simple.

167 ore, previous studies in which only a single PCR primer pair was used may have significantly underest

168 A novel PCR primer pair was used to detect the presence of cmeC

169 ved and replaced with sequences to match any PCR primer pair.

170 d regions for 48 DNA samples and hundreds of PCR primer pairs (producing up to 23,040 PCR products) d

171 uences were used to design 13 taxon-specific PCR primer pairs for those taxa most commonly implicated

172 However, designing subtype-specific PCR primer pairs is a very challenging task: on one hand

173 Detection limits of the 26 PCR primer pairs previously described for detection of H

174 d by endonuclease restriction maps, we chose PCR primer pairs that approximated and framed junctions

175 In this work, we identified four PCR primer pairs that collectively amplified cdt genes i

176 We designed PCR primer pairs to amplify genomic DNA flanking each of

177 demonstrate this principle, we designed 384 PCR primer pairs to conserved exonic regions flanking in

178 ch information was unavailable, selection of PCR primer pairs was guided by general insights gleaned

179 A set of four species-specific real-time PCR primer pairs were also designed, capable of detectin

180 fore confound standard-design "RNA-specific" PCR primer pairs which rely, for cDNA versus gDNA specif

181 nments of these genes, and designs potential PCR primer pairs.

182 ific reverse transcription primers, specific PCR primers pairs, TaqMan probe, double-hairpin probe an

183 es using specific polymerase chain reaction (PCR) primer pairs.

184 monstrate its utility in compiling Multiplex PCR primer panels for detection and differentiation of v

185 Here, we have used microarrays and a qRT-PCR primer platform covering 1,880 genes encoding transc

186 o-dT adapter incorporating a dUTP-containing PCR primer primes first-strand cDNA synthesis incorporat

187 We compared seven TaqMan real-time PCR primer/probe sets (three designed at this institutio

188 ns that includes programs for analysis of RT-PCR primer/probe sets, effectors of RNAi, microarrays, a

189 ain reaction (RT-PCR), novel quantitative RT-PCR primers/probe were developed, and whole genome seque

190 Researchers can select PCR primers, probes and antisense oligonucleotides, find

191 However, standardized real-time PCR primers, probes, and standards are unavailable, and

192 romosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s)

193 pplications such as simplified templates for PCR primers, randomized sequencing and DNA based devices

194 me polymorphism and oligonucleotide probe or PCR primer reactivity are useful for the detection of sp

195 small-subunit ribosomal DNA (16S rDNA)-based PCR primers recommended for use in the identification of

196 ve nucleotides included at the 3'-end of the PCR primers result in additional genome reduction as com

197 repeat clustering, polymorphism prediction, PCR primer selection, data visualization and data downlo

198 We designed new PCR primer sequences for the markers selected for multip

199 Our study provides PCR primer sequences to all of the known human miRNA pre

200 hisms (SNPs) and generates CAPS and/or dCAPS PCR primer sequences.

201 cribed, published polymerase chain reaction (PCR) primer sequences are reported, and validation studi

202 uencing reaction to proceed, with the excess PCR primer serving as the sequencing primer.

203 ed for enteric caliciviruses by using six RT-PCR primer sets designed for the detection of human NLVs

204 PyV in immunocompetent SCC patients, we used PCR primer sets directed against the large T (LT) antige

205 The design of PCR primer sets for PAMP is challenging because a large

206 We designed PCR primer sets targeting clades of the xoxF gene, and u

207 Selective PCR primer sets targeting this sequence were evaluated f

208 vities (limits of detection) of 13 published PCR primer sets were compared using DNA extracted from s

209 Exon-spanning PCR primer sets were identified by serial testing of pri

210 For the latter two PCR primer sets were used as these can affect the niche

211 The PCR primer sets were validated with 416 isolates of stap

212 equenced and four polymerase chain reaction (PCR) primer sets flanking (CA/GT)n sequences were constr

213 tivities of 2 TTV polymerase chain reaction (PCR) primer sets showed that the majority of samples wer

214 A PCR assay using serotype-specific PCR primers showed that 3 out of 20 LJP patients surveye

215 rphisms (SSAPs) amplify the region between a PCR primer site near the end of an element and an adjace

216 Using a single DOP-PCR primer, SNP loci spread throughout a genome can be a

217 in one of the two polymerase chain reaction (PCR) primers so that the restriction endonuclease cuts i

218 Using universal PCR primers, some 80 fragments of retroelement reverse t

219 hain CDR3-length distribution analysis using PCR primers specific for 23 Vbeta families performed in

220 Clinical isolates were screened with PCR primers specific for either mef(A) or mef(E) and for

221 , we expanded the repertoire of quantitative PCR primers specific for MCPyV to improve the detection

222 as determined in each of the clones by using PCR primers specific for regions unique to each plasmid,

223 n each step several nucleotides are changed, PCR primers specific for such alterations can be designe

224 PCR primers specific for the 16S ribosomal DNA (rDNA) of

225 PCR primers specific for the recently described antimicr

226 PCR primers specific for this inserted region confirmed

227 Using PCR primers specific for vector sequences, we amplified

228 PCR primers specific to both clades were used to generat

229 PCR primers specific to individual ORFs from Shewanella

230 PCR primers specific to the Reticulamoeba clade confirm

231 PCR primers targeting loci in the current Burkholderia c

232 A set of PCR primers targeting the identified genes is then desig

233 PCR primers targeting these two genes were designed and

234 gn and testing of polymerase chain reaction (PCR) primers targeting isoA, the gene encoding the activ

235 It uses target-specific pairs of PCR primers that are physically separated by surface imm

236 The ROC method entails using PCR primers that contain regions of RNA sequence that ca

237 be a novel strategy for design of diagnostic PCR primers that exploited this rapid draft bacterial ge

238 PCR primers that flanked the deleted TbetaR-I region amp

239 apture method to discover, clone and develop PCR primers that permit the use of simple sequence repea

240 ovel degenerate oligonucleotide-primed (DOP) PCR primers that preferentially amplify mouse genomic se

241 were selected to generate a set of real-time PCR primers that target 93.6% of all bacterial 16S rRNA

242 iplex PCR assay with four sets of degenerate PCR primers that target the E1A, fiber, and hexon genes

243 Further, we find that the PCR primers that they utilized for amplification of the

244 developed generic reverse transcription (RT)-PCR primers that will amplify all human enterovirus sero

245 entifies a set of polymerase chain reaction (PCR) primers that will bind across the alignment.

246 When suitable ligands are attached to the PCR primers, the cruciform structures can be detected by

247 By varying the design of the PCR primers, the protocol is easily adapted to perform o

248 is RT primer is compatible with this reverse PCR primer, thus eliminating confounding signal from con

249 To amplify breakpoints with PCR, primers tiling specified target regions are careful

250 mploys the covalent attachment of one of two PCR primers to a particle surface either directly during

251 ence (EOC00242) was found and used to design PCR primers to acquire the full-length cDNA sequence.

252 tool to identify signature genes and design PCR primers to amplify related genes from uncultured pha

253 rays comes from the cost of synthesizing the PCR primers to amplify the desired DNA.

254 equences directly as the sense and antisense PCR primers to amplify the original DNA fragment.

255 dentify candidate signature genes and design PCR primers to amplify those genes from environmental sa

256 hat can facilitate the picking of mismatched PCR primers to artificially introduce or abolish a restr

257 uence data allowed for the design of precise PCR primers to clone the desired gene target fragments i

258 0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclea

259 sents two sets of degenerate oligonucleotide PCR primers to facilitate the cloning of cytosolic MDH (

260 ligation-mediated PCR (LM-PCR), using nested PCR primers to increase the specificity and sensitivity

261 Sperm DNA typing using allele-specific PCR primers to selectively amplify recombinant TAP2 mole

262 Our method can also be used to design PCR primers to specifically amplify homologs in the same

263 ication experiments by designing appropriate PCR primers to test cDNA libraries or applying RT-PCR, e

264 subsequences (such as parts of adapters and PCR primers used during the sample and sequencing librar

265 The prevalence varied by region, PCR primers used, publication calendar period and Gleaso

266 y been developed for the automatic design of PCR primers using DNA fragments that are specific to ind

267 To test this hypothesis, we have used the PCR primers utilized in the original report to amplify C

268 A complete set of PCR primers was created for all 4290 annotated open read

269 With specific diagnostic RT-PCR primers we identified several identical nucleotide s

270 Using BIOMED-2 PCR primers, we detected a high frequency of clonally re

271 ining and use of ortholog-specific universal PCR primers, we found that the PSY duplication is preval

272 The specificities of the real-time PCR primers were 94.2% for P. vivax (49/52) and 100% for

273 PCR primers were designed based on two unique features o

274 nces predicted from the SynMeta metagenomes, PCR primers were designed for amplification of one plasm

275 Oligonucleotide PCR primers were designed for each polymorphic locus suc

276 PCR primers were designed for either the broad-spectrum

277 of this amplicon, additional target-specific PCR primers were designed for use in subsequent rounds o

278 Degenerate PCR primers were designed from the amino acid sequences

279 Serotyping PCR primers were designed from variable regions of the L

280 Orthopoxvirus assay, consensus Orthopoxvirus PCR primers were designed to amplify 266-281 base-pair (

281 PCR primers were designed to amplify type-specific GBS c

282 PCR primers were designed to detect all Eurasian-African

283 Six multiplex-compatible PCR primers were designed to distinguish Streptococcus p

284 Eighteen sets of virus-specific multiplex PCR primers were developed based on the conserved sequen

285 ARMS PCR primers were developed to recognize polymorphisms th

286 nomic repeats were isolated, in context, and PCR primers were generated against the flanking single-c

287 nd fhbA2, were delineated, and type-specific PCR primers were generated to allow for rapid differenti

288 PCR primers were screened for their capacity to discrimi

289 PCR primers were used for amplification of the 3.4 kb 5'

290 Degenerate PCR primers were used on large DNA pools (n = 2,025 T-DN

291 Broad-range PCR primers were used to amplify part of the groESL oper

292 Degenerate polymerase chain reaction (PCR) primers were designed based on two conserved amino

293 Amplicons are derived from PCR primers which amplify short (<140-bp) regions of the

294 The conserved sequences were used to design PCR primers which efficiently amplified these polymorphi

295 on the 180 samples using 2 different sets of PCR primers (which target 2 different genes).

296 ntroduced by the unique design of one of the PCR primers, which carries a special 5'-flap sequence.

297 ed protein fragments, and specially designed PCR primers, which introduce N- and C-terminal markers f

298 ple species enabled the design of "universal PCR primers," which can be used to amplify the correspon

299 Scorpions are fluorogenic PCR primers with a probe element attached at the 5'-end

300 probe, a pair of Padlock probes or a pair of PCR primers with an optional TaqMantrade mark probe (i.e