ライフサイエンスコーパス: PDO

1 PDO and AMO are also contributing to this multi-time sca

2 PDO cultures were established from surgical specimens an

3 PDO has been proposed as an alternative to anastomosis t

4 PDO has long served as a model for understanding ROs des

5 PDO in vitro chemosensitivities to standard-of-care chem

6 PDO mixtures were directly analyzed by mass spectrometry

7 PDO pre-screening can refine therapy by identifying (in)

8 PDO with neoprene-based glue is a safe technique that eq

9 PDOs demonstrated unique apoptotic and tumor-stroma modu

10 PDOs poorly-matched to the neoadjuvant regimen received

11 PDOs preserve the histological and molecular heterogenei

12 PDOs recapitulate tumor histology and contain genetic al

13 PDOs recapitulated patient tumor intrinsic properties an

14 PDOs were established from pre-treatment biopsies in a m

15 PDOs with actionable biomarkers showed clear differentia

16 PDOs' response towards the single substances of the FLOT

17 ssed by multivariate methods to classify 183 PDO honeys produced in three regions of Spain (Liebana,

18 ion and CIELAB parameters determination of 9 PDO EVOOs from Spain were developed.

19 Due to high price as a PDO product, commercial products of "Fava Santorinis" ar

20 Mozzarella di Bufala Campana (MBC) is a PDO cheese produced from whole buffalo milk in specific

21 to demonstrate the clinical feasibility of a PDO-guided precision medicine framework of care.

22 e effects that grape-must caramel has upon a PDO vinegar's final quality.

23 el amounts under 2% v/v in both Balsamic and PDO wine vinegars.

24 aluable archives to detect regional ENSO and PDO impacts, and their interaction with the Asian-Austra

25 We show that ENSO and PDO together predicted (i) maximum sea-surface temperatu

26 the molecular heterogeneity of PDAC PDX and PDO models and comparison with matched human tumour rema

27 'trios' of matched primary tumour, PDX, and PDO.

28 y and the NINO 3, NINO 3.4, NINO 4, SOI, and PDO indices.

29 ted that heterotrophic bacteria with SQR and PDO can rapidly oxidise sulfide to thiosulfate and sulfi

30 or using heterotrophic bacteria with SQR and PDO for sulfide bioremediation.

31 Recombinant E. coli cells with SQR and PDO rapidly oxidised sulfide to thiosulfate and sulfite.

32 generated in 94%, transcriptomes in 74%, and PDOs in 50%.

33 ed EGC (n = 120) were taken into culture and PDOs expanded.

34 SOS1 degradation in both CRC cell lines and PDOs with excellent specificity.

35 investigations highlight that while PDXs and PDOs may serve as tractable and transplantable systems f

36 model for use in the authentication of Arnad PDO lard.

37 and compared to patient responses to assess PDO predictive values and map the drug sensitivity lands

38 ery low levels of sugars detected, authentic PDO Grana Padano could be safely included in the diet of

39 ethod allowing us to detect and authenticate PDO "Fava Santorinis".

40 ctiveness alternative method to authenticate PDO "Fava Santorinis" and to quantitatively detect adult

41 Neoprene-based PDO, however, triples the risk of diabetes at 1 and 3 ye

42 SVM also allows the differentiation between PDOs.

43 olved in substrate hydroxylation employed by PDO and either NDO or BZDO.

44 oal of determining whether esophageal cancer PDOs created from treatment naive patients can model or

45 nter were used to generate esophageal cancer PDOs, which were treated with standard-of-care chemother

46 In the exploratory cohort, PDOs response to single 5-FU and FLOT combination treatm

47 fusions between the non-heme iron containing PDO and rhodanese, a thiol sulfurtransferase, exist in s

48 ke center was oxidized in either WT or D178N PDO, NO bound to the mononuclear iron in the presence or

49 oreductase (SQR) and persulfide dioxygenase (PDO) genes.

50 none oxidoreductase, persulfide dioxygenase (PDO), rhodanese, and sulfite oxidase and converts H2S to

51 Phthalate dioxygenase (PDO) and its reductase (PDR) are parts of a two-componen

52 Phthalate dioxygenase (PDO) and its reductase are parts of a two-component Ries

53 The metalloenzyme phthalate dioxygenase (PDO) contains two iron-based sites.

54 4)N]histidine-labeled phthalate dioxygenase (PDO) from Pseudomonas cepacia were recorded and found to

55 , has two components: phthalate dioxygenase (PDO), a multimer with one Rieske-type [2Fe-2S] and one F

56 t has two components: phthalate dioxygenase (PDO), a multimer with one Rieske-type [2Fe-2S] and one m

57 ituted derivatives of phthalate dioxygenase (PDO).

58 General model was not suited to distinguish PDO red wines from samples, with similar chemical finger

59 a, the model was effective in distinguishing PDO and non-PDO mozzarella, particularly when non-PDO ch

60 lesion edge areas had fewer and less diverse PDOs than healthy tissues, possibly due to metabolic by-

61 Hydrogel mechanical properties control EAC PDO formation, growth, proliferation, and activation of

62 ion of patient-derived EAC 3D organoids (EAC PDOs).

63 minate the wine vinegar category within each PDO, for which SVM models obtained better results (>92%

64 EGC PDOs reflected patients' diverse responses to single che

65 In vitro drug testing of EGC PDOs has a high predictive accuracy in classifying patie

66 In our study, we establish PDO cultures from different BLCa stages and grades.

67 argeted SIFT-MS volatile fingerprints of ewe PDO cheeses in a real farm model, using mixOmics methods

68 on were identified by determination of exact PDO molecular masses and tandem mass spectrometry via co

69 Pharmacotyping rapidly-expanded PDOs was completed in a median of 48 (range 18-102) days

70 In conclusion, F-PDO thus acts as a novel (pro)-fluorescent probe for mon

71 s, these photoreduced adducts derived from F-PDO are characterized by a bright yellowish emission due

72 l data from rice analysis were evaluated for PDO certification of Argentine brown rice.

73 ) is an interesting analytical technique for PDO certification, since it provides fast multielemental

74 We have identified four PDO series that were generated through the breakdown of

75 Fifty-four PDOs were generated from 87 pretreated patients (take-on

76 the product forms rapidly, its release from PDO is slow in these reactions with oxygen that do not i

77 e and caprine species and authenticate Greek PDO Feta cheese.

78 lysulfides with glutathione to produce GSSH; PDO oxidises GSSH to sulfite; sulfite spontaneously reac

79 In summary, HGSOC PDO cultures provide validated genomic models for studie

80 otocol allows for long-term culturing of HR+ PDOs that retain estrogen receptor expression and show r

81 tution of D178 with alanine or asparagine in PDO resulted in proteins with significantly increased Fe

82 , whereas only the reduced Rieske centers in PDO become oxidized during production of DHD.

83 When the Rieske centers in PDO were oxidized, D178N and D178A substitutions disrupt

84 etween the Rieske and mononuclear centers in PDO.

85 ented, to address authenticity challenges in PDO feta cheese which is legally manufactured by the mix

86 The effects of substituting D178 in PDO with alanine or asparagine on the reactivity of the

87 Aspartate D178 in PDO, located near its ferrous mononuclear center, is hig

88 Aspartate D178 in PDO, which lies between the Rieske [2Fe-2S] center of on

89 lst the percentage of 18:2 n-6 was higher in PDO beef, relative to other two meats.

90 PGI veal, and that of 18:1 c9 was higher in PDO veal, whilst the percentage of 18:2 n-6 was higher i

91 higher, and the n-6/n-3 ratio was lower, in PDO veal, relative to the other two meats.

92 o selumetinib, gefitinib, and regorafenib in PDOs and to 5-fluorouracil and oxaliplatin in PDX in viv

93 thenticate milk and dairy products including PDO Feta cheese and to quantitatively detect its sheep m

94 nfirm the geographical origin of 177 Italian PDO (Protected Denomination of Origin) olive oils and 86

95 f C(4) plants allowed to distinguish Italian PDO from Spanish hams.

96 With a molecular mass of 200 kDa, PDO is one of the largest proteins studied to date by pa

97 pulsed and CW ENDOR spectra of 57Fe-labeled PDO, and the resulting data were used to derive the full

98 Lambrusco PDO (Protected Designation of Origin) wines coming from

99 content was a discriminant marker of Liebana PDO and El Bierzo, that could also be differentiated fro

100 vation of 30 patient-derived organoid lines (PDOs) from tumors arising in the pancreas and distal bil

101 he substrate and PDR was synergistic, making PDO fully catalytically active.

102 lysis of drug response profiles with matched PDO genomic analysis is used to determine enrichment thr

103 By merging MBC-PDO samples with non-PDO samples of buffalo mozzarella p

104 Two typical Mediterranean PDO salami produced in Calabria, a region in the Souther

105 PGI veal, Mertolenga-PDO veal and Mertolenga-PDO beef were analysed for cholesterol (HPLC-UV), alpha-

106 Tradicional do Montado"-PGI veal, Mertolenga-PDO veal and Mertolenga-PDO beef were analysed for chole

107 reduced Rieske center in both WT and mutant PDO.

108 the oxidation of the Rieske center in native PDO(red) [which contains Fe(II) at the mononuclear site]

109 Addition of PDR(ox) to native PDO(red) resulted in a large fraction of the Rieske cent

110 the 1930s (positive PDO) and 1950s (negative PDO) drought.

111 In a negative PDO phase, the Kuroshio Extension Current (KEC) is stron

112 stern and tropical Pacific cooling (negative PDO).

113 As a result, annual cohorts from negative PDO years may face a lack of resources, whereas cohorts

114 was effective in distinguishing PDO and non-PDO mozzarella, particularly when non-PDO cheeses were m

115 nd non-PDO mozzarella, particularly when non-PDO cheeses were made outside the MBC reference area.

116 By merging MBC-PDO samples with non-PDO samples of buffalo mozzarella produced both inside a

117 rrects for this error suggests that observed PDO impacts-including the ongoing multidecadal drought i

118 y and efficacy of pancreatic duct occlusion (PDO) with neoprene-based glue in selected patients under

119 hat can be identified using SHMT analysis of PDO radiation responses.

120 n, thus offering a way for authentication of PDO sample origin.

121 se maturation) as markers of authenticity of PDO cheeses in terms of ripening time and milk admixture

122 technique for detecting the authenticity of PDO cheeses.

123 al origins and confirmed the authenticity of PDO labelling as Granada product with the determination

124 timal one for evaluating the authenticity of PDO MBC cheese products.

125 A categorization of PDO EVOOs was proposed using the following 7 phenolic co

126 uclear site and the reduced Rieske center of PDO suggest that the two sites are at least 12 A apart,

127 electrons from NADH to the Rieske center of PDO, and the Rieske center supplies electrons to the mon

128 Complexes of PDO(KF1) with phthalate and terephthalate revealed that

129 with emphasis on an authenticity control of PDO Feta cheese products.

130 Ex-vivo generation of PDO for pharmacotyping may serve as predictive biomarker

131 aphical discrimination and identification of PDO olive oils.

132 This emphasizes the importance of PDO subunit interaction in catalysis.

133 rate interacts with the mononuclear metal of PDO and provide the first structural characterization of

134 d its use is mandatory for the production of PDO cheeses such as Parmigiano Reggiano and Grana Padano

135 ry use of animal rennet in the production of PDO cheeses.

136 O(red) each results in greater reactivity of PDO with O(2).

137 Reduction of PDO by reduced PDR was also slower in the D178A and D178

138 rmly labeled with 15N but not in a sample of PDO labeled with [delta-15N,epsilon-14N]histidine, but t

139 he defined protocol is applied to samples of PDO Nimes olive oil.

140 solution than those obtained with samples of PDO, our analysis nevertheless permits the conclusion th

141 s acidity and its authentication in terms of PDO (Protected Designation of Origin) and PGI (Protected

142 fects is presented and discussed in terms of PDO subunit cooperativity.

143 th elemental (REE) fingerprint variations of PDO (Protected Designation of Origin) "Fava Santorinis"

144 Our function matched 91% of PDOs to a standard-of-care regimen (9% pan-resistant).

145 responses in the clinic, but the ability of PDOs to predict responses to chemoradiation in cancer pa

146 Rapid development of PDOs from patients undergoing surgery for PDAC is eminen

147 maintain viability and allowed generation of PDOs with 77% success.

148 A framework for rapid pharmacotyping of PDOs was established across a multi-institutional consor

149 A classifier based on FLOT response of PDOs was established in an exploratory cohort (n = 13) a

150 ons maintained the differentiation status of PDOs and enabled rapid assessment of drug response and t

151 t tumor intrinsic properties and a subset of PDOs can be propagated for long-term culture (LT-TNBC).

152 lung tumors, coupled with ex vivo testing of PDOs, identifies continuous AXL, TGFbeta, and JAK1-STAT3

153 However, the use of PDOs created from treatment-naive patient biopsies for p

154 r clinical response, suggesting the value of PDOs in prediction of clinical response to new therapeut

155 f cell wall pectin-derived oligosaccharides (PDOs) in three regions of Botrytis cinerea-infected toma

156 sequently, the method is preliminary used on PDO Nimes and Moroccan oils to evaluate the feasibility

157 Patient-derived organoid (PDO) can recapitulate the biological features of the pri

158 enografts and LUAD patient-derived organoid (PDO) intracardiac injection metastasis in vivo models.

159 r activity in patient-derived CRC organoids (PDO).

160 Patient-derived organoids (PDO) generated from bladder cancer samples before and af

161 responsiveness of patient-derived organoids (PDO) generated from normal human intestines and rectal t

162 Patient-derived organoids (PDO) obtained from tumors with high AXL and JAK1 were se

163 ografts (PDX) and patient-derived organoids (PDO) serve as promising tools to identify new drugs with

164 and breast cancer patient-derived organoids (PDO) with a focus on TNBCs.

165 yc in HNSCC using Patient-Derived Organoids (PDO), Cell Line-Derived Xenografts, and Patients-Derived

166 us propagation of patient-derived organoids (PDOs) and matching CAFs, from primary and metastatic HR+

167 Patient derived organoids (PDOs) are three-dimensional (3D) tissue cultures that pr

168 ce indicates that patient-derived organoids (PDOs) can predict drug responses in the clinic, but the

169 3D patient-derived organoids (PDOs) have been utilized to evaluate potential therapies

170 C) cell lines and patient-derived organoids (PDOs).

171 es with the protected designation of origin (PDO) 'Kaki Ribera del Xuquer' Valencia (Spain).

172 no Reggiano protected designation of origin (PDO) cheese inherently exhibits variability due to the c

173 da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has

174 ong-ripened Protected Designation of Origin (PDO) Coppa Piacentina.

175 Honey with Protected Denomination of Origin (PDO) could be an attractive target for fraudsters.

176 an Italian protected designation of origin (PDO) dry-cured product obtained from the muscle of pork

177 the mark of Protected Designation of Origin (PDO) has been assigned to MBC in relation to its geograp

178 ldwide, therefore its designation of origin (PDO) is very useful.

179 Aosta Arnad Protected Designation of Origin (PDO) lard.

180 duction of Protected Denomination of Origin (PDO) Mozzarella di Bufala Campana (MBC) cheese has promo

181 ucing it as Protected Designation of Origin (PDO) or Protected Geographical Indication (PGI) product

182 n under the protected designation of origin (PDO) standardized procedures leads to more quality produ

183 proteins in Protected Designation of Origin (PDO) Teruel dry-cured ham have been identified by nanoli

184 negars with Protected Designation of Origin (PDO) were described and compared for the first time by g

185 roduct with protected designation of origin (PDO) which includes six monofloral honeys and two multif

186 of Spanish Protected Designation of Origin (PDO) wine vinegars.

187 inis", is a protected designation of origin (PDO) yellow split pea species growing only in the island

188 d in Spain, protected designation of origin (PDO), and saffron packaged in Spain of unknown origin, l

189 ed with the Protected Designation of Origin (PDO), ensuring their quality and uniqueness.

190 hose with a protected designation of origin (PDO), for unifying the final colour of different batches

191 carrying a Protected Designation of Origin (PDO).

192 EVOOs) with protected designation of origin (PDO).

193 e final product and undervalues the original PDO cheese.

194 odulated by the Pacific Decadal Oscillation (PDO) and increased vertical wind shear.

195 related to the Pacific Decadal Oscillation (PDO) and Pacific North American mode (PNA).

196 fluenced by the Pacific Decadal Oscillation (PDO) and Siberian high (SH).

197 relating to the Pacific Decadal Oscillation (PDO) and the Arctic Oscillation (AO).

198 ibutable to the Pacific Decadal Oscillation (PDO) and the Atlantic Multidecadal Oscillation (AMO).

199 iability of the Pacific Decadal Oscillation (PDO) and the Atlantic Multidecadal Oscillation (AMO).

200 tation, and the Pacific Decadal Oscillation (PDO) in the year of collection affected plant height, le

201 apping onto the Pacific Decadal Oscillation (PDO) index and North Pacific Gyre Oscillation (NPGO) ind

202 tuations in the Pacific Decadal Oscillation (PDO) index have slowed down markedly over the observatio

203 ns) in negative Pacific Decadal Oscillation (PDO) years, as well as with sea-level-rise and surface w

204 illation (NAO), Pacific Decadal Oscillation (PDO), and Atlantic Multidecadal Oscillation (AMO).

205 illation (NAO), Pacific Decadal Oscillation (PDO), Arctic Oscillation (AO), and Atlantic Multi-decada

206 ty, such as the Pacific Decadal Oscillation (PDO), are poorly understood.

207 The Pacific Decadal Oscillation (PDO), as the primary variability mode in the NE Pacific

208 e phases of the Pacific Decadal Oscillation (PDO), as well as highlight potential concerns for marine

209 ns, such as the Pacific Decadal Oscillation (PDO), have influenced these fisheries, while diminished

210 te variability [Pacific Decadal Oscillation (PDO), North Pacific Gyre Oscillation (NPGO), Arctic Osci

211 ely tracked the Pacific Decadal Oscillation (PDO), which is driven on these timescales by an 18.6-yea

212 e driven by the Pacific Decadal Oscillation (PDO).

213 tion (NAO), and Pacific Decadal Oscillation (PDO).

214 istent with the Pacific Decadal Oscillation (PDO).

215 (ENSO) and the Pacific Decadal Oscillation (PDO).

216 , driven by the Pacific Decadal Oscillation (PDO).

217 c came with the Pacific Decadal Oscillation (PDO).

218 y driven by the Pacific Decadal Oscillation (PDO).

219 Finally, we leveraged our PDO collection as a platform for drug validation and com

220 ls in hard cheese, specifically Grana Padano PDO cheese.

221 od was applied to 59 samples of Grana Padano PDO cheese: galactose showed the highest concentration a

222 Reduced PDO (PDO(red)) that lacks Fe(II) at the mononuclear metal sit

223 biomarkers of long-ripened Coppa Piacentina PDO.

224 se to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch intended for tendo

225 The casein fraction of 13 Portuguese PDO cheeses were analysed using Urea-PAGE and reverse ph

226 nental-scale patterns of the 1930s (positive PDO) and 1950s (negative PDO) drought.

227 15/17 ENSO event was coupled with a positive PDO and resulted in high SST and Acanthaster abundances

228 During a positive PDO phase, however, the KEC weakens facilitating the neo

229 of resources, whereas cohorts from positive PDO years may find sufficient resources during their piv

230 Asynchrony between the positive PDO and negative ENSO (i.e., La Nina) was associated wit

231 By contrast, synchrony between the positive PDO and positive ENSO (i.e., El Nino) was associated wit

232 second case study compares 1,3-propanediol (PDO) produced from fossil fuels and from biomass, which

233 Here we purified PDO(KF1) from Comamonas testosteroni KF1 and found that

234 Reduced PDO (PDO(red)) that lacks Fe(II) at the mononuclear meta

235 However, in reactions of reduced PDO with O(2), only about 0.5 DHD is formed per Rieske c

236 s proposed that, during oxidation of reduced PDO, each of two Rieske centers on separate subunits tra

237 nding of phthalate and/or PDR(ox) to reduced PDO-APO increases the reactivity of the Rieske center wi

238 -olfactive complexity of Parmigiano Reggiano PDO cheese, with spicy, umami and intense vegetal aromat

239 Grana Padano (GP) cheese is a renowned PDO Italian cheese whose nutritional characteristics and

240 The B. phytofirmans PRF exhibited robust PDO activity and preferentially catalyzed sulfur transfe

241 s uni-floral honey and producers of the same PDO.

242 lacks Fe(II) at the mononuclear metal site (PDO-APO) reacts slowly with O(2) (1.4 x 10(-3) s(-1) at

243 hich is not allowed to produce milk for some PDO cheeses as Parmigiano-Reggiano.

244 l in Balsamic vinegars of Modena and Spanish PDO wine vinegars.

245 sis method for the authentication of Spanish PDO wine vinegars.

246 es (aged and sweet) belonging to the Spanish PDOs "Vinagre de Jerez", "Vinagre de Montilla-Moriles" a

247 pring ENSO, summer NAO, and winter or spring PDO accounted for 40-54% of the variation in grasshopper

248 Our study suggests that PDO can recapitulate patient responses in the clinic wit

249 These data imply that PDOs predict LARC patient responses in the clinic and ma

250 The PDO and NPGO also show time-dependent skill as indices o

251 PDO), and increasing correlation between the PDO and NPGO patterns.

252 ir in vitro PDO responses, demonstrating the PDO's ability to mirror clinical response, suggesting th

253 gression model incorporating indices for the PDO and AO, as well as the declining trend, can account

254 hout PDO (El Bierzo) separated well from the PDO clusters.

255 However, the PDO is a climate index based on sea surface temperature

256 usively via N(delta) for both ligands in the PDO center.

257 that the main multidecadal variations in the PDO index during the twentieth century, including the on

258 and the kinetics of electron transfer in the PDO system indicated that each of these metal ions binds

259 tential fluorophores and their values in the PDO vinegars.

260 ity through externally forced changes in the PDO.

261 e co-occurrence of warm (cold) phases of the PDO and MEI with negative (positive) upwelling anomalies

262 in duration, suggesting the influence of the PDO and PNA teleconnections.

263 Since 2004, the strengthening of the PDO and SH explains approximately 54% and 18% of the aut

264 haracteristic, irregular oscillations of the PDO arise from a collection of mechanisms involving ocea

265 h PDR(ox) and phthalate present, most of the PDO(red) (approximately 80-85%) oxidizes at 42 s(-1), wi

266 ian Low (a leading atmospheric driver of the PDO), and increasing correlation between the PDO and NPG

267 in-wide warming and a favorable phase of the PDO, both the intensity and frequency of such cyclones a

268 wever, little is known about the role of the PDO-rhodanese fusion (PRF) proteins in sulfur metabolism

269 idated with 131 samples produced outside the PDO regions and El Bierzo.

270 rica indicate that the NPGO, rather than the PDO, explains salmon survival since the 1980s.

271 Two wave trains related to the PDO and the AMO appear to produce anomalous surface-air

272 llation (NPGO), whereas EPW is linked to the PDO, different indicators of northeast Pacific Ocean eco

273 h the AMO and negatively correlated with the PDO.

274 dine and conformation of subunits within the PDO multimer to create a more open structure with more s

275 The PDOs were isolated from lesion centers (extensively mace

276 t informative variables to differentiate the PDOs.

277 In the PDOs, depletion or pharmacologic inhibition of NPEPPS in

278 plete response, correlate closely with their PDO D0 values.

279 Overall, this study validates TNBC PDOs as robust models for understanding breast cancer bi

280 s demonstrates that fluorophthalate binds to PDO with a handedness, i.e., with the fluorine label fac

281 Thus, the binding of phthalate or PDR(ox) to PDO(red) each results in greater reactivity of PDO with

282 trials should test the use of pre-treatment PDOs to identify specific, targeted therapies for indivi

283 in interventional precision oncology trials, PDO collection already provides powerful opportunities f

284 Samples from two PDOs were analyzed by LIBS coupled to spark discharge.

285 Fifty-one patients underwent PDO and 49 PJA.

286 A subsequent prospective study using PDO pharmacotype matching could improve oncologic outcom

287 utics to target pancreatic tumor cells using PDOs.

288 drug screening pipeline is implemented using PDOs, testing standard-of-care and FDA-approved compound

289 Studies validating PDOs as a promising predictive biomarker are ongoing.

290 In vitro PDO pharmacotyping can inform PDAC therapy selection.

291 nt treatment were compared to their in vitro PDO responses, demonstrating the PDO's ability to mirror

292 Further, while PDOs are known to reflect the cognate in vivo biology of

293 E. coli cells with PDO and SQR-C94S, an active site mutant of the rhodanese

294 The use of (63)Cu(II), in combination with PDO obtained from cultures grown on media enriched in (1

295 ore Sardo cheese produced in compliance with PDO specifications which allow the use of only native LA

296 re (FRS) >15% (>=6 points) were treated with PDO using neoprene glue (study cohort); patients with FR

297 les (18) produced in a fourth region without PDO (El Bierzo) separated well from the PDO clusters.

298 dihydrodiol formation approached 100% in WT PDO but was only approximately 16% in D178A and approxim

299 variants, as opposed to that observed in WT PDO.

300 and D178N compared to that of wild-type (WT) PDO (>10(2)-fold) can be ascribed to the cumulative effe