ライフサイエンスコーパス: PEDF

1 PEDF blocked VEGF-induced phosphorylation of extracellul

2 PEDF bound the PEDF-R ectodomain L4 (Leu(159)-Met(325))

3 PEDF bound to LRP6, a Wnt coreceptor, with high affinity

4 PEDF distribution was evaluated by immunofluorescence of

5 PEDF expression and/or regulation during melanoma develo

6 PEDF expression was consistently decreased in invasive a

7 PEDF expression was reduced in 12-HETE-treated rMCs, ast

8 PEDF immunostaining around CNV lesions diminished after

9 PEDF inhibited adipogenesis and promoted osteoblast diff

10 PEDF or DHA alone did not produce a significant increase

11 PEDF overexpression suppressed thrombospondin-1 levels i

12 PEDF up-regulates expression of tight junction-associate

13 PEDF up-regulation of full-length presenilin 1 (Fl.PS1)

14 PEDF was lost in thicker melanomas (P = 0.003), and corr

15 PEDF, VEGFR3, beta-3 tubulin, CD45, CD11b, and F4/80 exp

16 PEDF-dependent SOD2 expression in PanIN lesions was reca

17 PEDF-null mice, however, demonstrated enhanced early fib

18 inear trends of greater reductions in PAI-1, PEDF, and VEGF.

19 oduction of BrM components, including TSP-1, PEDF, and tropoelastin in vitro and increased the antian

20 Combined decline rate of KLK-7, PEDF, MDC and ANGPTL4 by Week 16 represented biomarkers

21 The factor involved is likely PEDF, as a PEDF-antiserum blocked the repulsing action.

22 Recently, a PEDF receptor has shown an elevated binding affinity for

23 in turn is critical for NFkappaB activation, PEDF-dependent apoptosis, and anti-angiogenesis.

24 Thus, adding PEDF enhanced Wnt-beta-catenin signal transduction in hu

25 In addition, PEDF+DHA accelerated corneal wound healing, selectively

26 In addition, PEDF-Tg mice with OIR had significantly lower vascular l

27 1 associated with VEGFR1 within 15 min after PEDF treatment.

28 e in VEGFR3 (57.5% reduction, P = 0.001) and PEDF protein expression (64% reduction, P < 0.001).

29 MMP-2, MMP-9, and PEDF protein levels were determined by ELISA.

30 alculations, these data implicate VEGF-A and PEDF as key RPE-derived factors promoting preservation o

31 rm homooligomers under basal conditions, and PEDF dissociates the homooligomer to activate the recept

32 lculations, these data may implicate HGF and PEDF as key factors promoting the preservation of retina

33 d by osteoblasts, while MIA, LECT1, NGAL and PEDF are expressed by other calvarial cells.

34 In the in vitro cultures, NPD1 and PEDF+DHA induced a 3-fold increase in neurite outgrowth

35 s demonstrated to bind immobilized PEDF, and PEDF was shown to bind to immobilized C1q, in particular

36 ntravitreal injections of these peptides and PEDF in the rd1 mouse model of retinal degeneration decr

37 GF-E rapidly increased RPE permeability, and PEDF inhibited the VEGF-E response dose-dependently.

38 eu(232)) and internally truncated PEDF-R and PEDF-R4 (DeltaHis(203)-Leu(232)) retained phospholipase

39 ockdown by small interfering RNA (siRNA) and PEDF knockout in PEDF(-/-) mice induced activation of Wn

40 y i.p. injections) protocol in wild-type and PEDF-null mice.

41 of retinal NV through modulation of VEGF and PEDF expression and could provide a new therapeutic targ

42 ne the effects of NT on RPE-derived VEGF and PEDF expression in the context of passive smoking.

43 VEGF and PEDF expression was assessed by ELISA, Western blot and

44 VEGF and PEDF expression was assessed by ELISA, Western blot, and

45 The effects of 12-HETE on VEGF and PEDF expression were evaluated in Muller cells (rMCs), p

46 and nAChR-mediated upregulation of VEGF and PEDF were observed in RPE from rats exposed to NT.

47 and nAChR-mediated upregulation of VEGF and PEDF were observed in RPE from rats exposed to NT.

48 ent by altering the balance between VEGF and PEDF.

49 hMSCs, whereas the combination of Wnt3a and PEDF potentiated mineralization.

50 In wild-type animals, PEDF expression increased with pancreatitis and was more

51 l, which was ablated by the presence of anti-PEDF antibody.

52 treatment of the conditioned media with anti-PEDF antibodies allowed fhRPE cells to fully respond to

53 ch's membrane and promote the antiangiogenic PEDF to inhibit neovascularization.

54 ence of various amounts of complexes between PEDF and C4d in the SF from 30 RA patients, whereas none

55 produced PDGF-BB and TGFbeta, which blocked PEDF production in fibroblasts.

56 increase in permeability was blocked by both PEDF and the same kinase inhibitors.

57 Blockade of adipogenesis by PEDF suppressed peroxisome proliferator-activated recept

58 -catenin and uPAR expression were blocked by PEDF and by inhibitors of p38 and MEK.

59 red here an active cornea-TG axis, driven by PEDF-R activation, that fosters axon outgrowth in the co

60 l control of Sod2 expression was mediated by PEDF-induced NFkappaB nuclear translocation.

61 Differentiation to osteoblasts by PEDF resulted in a common pathway that involved PPARgamm

62 4T1-BR breast cancer cells was suppressed by PEDF expression, as supported by in vitro analyses as we

63 s suggested that, in arthritis patients, C4d-PEDF complexes found in sera arise from the joints, as w

64 ts cytotoxic effects on breast cancer cells, PEDF also exerted a prosurvival effect on neurons that s

65 sum, demonstrate induction of endogenous CNS PEDF production following demyelination, and make PEDF a

66 l-time PCR and microarray analyses confirmed PEDF down-regulation at the mRNA level in several melano

67 Purified HC.HA did not contain PEDF and TSP-1 but did contain IGFBP-1 and platelet fact

68 Similarly, continuous PEDF administration into the lateral ventricles of adult

69 In contrast, PEDF-Tg animals with oxygen-induced retinopathy (OIR) de

70 investigated the role of fibroblast-derived PEDF in melanoma progression.

71 feedback loop was discovered between DGAT1, PEDF, and GM130.

72 gative MEK5 mutant and Erk5 shRNA diminished PEDF-dependent apoptosis, inhibition of the endothelial

73 Using siRNA to selectively knock down PEDF-R in retina cells, we demonstrated that PEDF-R is e

74 1 nAchR, upregulated VEGF, and downregulated PEDF expression through nAChR in ARPE-19 cells.

75 a1 nAchR, upregulated VEGF and downregulated PEDF expression through nAChR in ARPE-19 cells.

76 Doxycycline elevated PEDF levels in plasma and did not affect the active and

77 idase Inhibitor, Clade F) gene which encodes PEDF (pigment epithelium-derived factor), a potent inhib

78 s showed a robust upregulation of endogenous PEDF in the corpus callosum upon lysolecithin-induced de

79 Our findings establish PEDF as both a metastatic suppressor and a neuroprotecta

80 ta-treated normal fibroblasts with exogenous PEDF decreased the expression of CAF markers and restore

81 se melanoma cell lines were found to express PEDF mRNA.

82 dying neurons adjacent to tumors expressing PEDF.

83 encoding pigment epithelium-derived factor (PEDF) (SERPINF1) was performed.

84 DNF), and pigment epithelium-derived factor (PEDF) and significantly lower concentrations of leukemia

85 usly that pigment epithelium-derived factor (PEDF) can, via gamma-secretase-mediated events, inhibit

86 BRIL) and pigment epithelium-derived factor (PEDF) defects cause types V and VI osteogenesis imperfec

87 acellular pigment epithelium-derived factor (PEDF) displays retina survival activity by interacting w

88 ment with pigment epithelium-derived factor (PEDF) in combination with docosahexaenoic acid (DHA) on

89 ith NPD1, pigment epithelial-derived factor (PEDF) in combination with docosahexaenoic acid (DHA) or

90 Pigment epithelium-derived factor (PEDF) is a multifunctional protein which was initially d

91 Pigment epithelium-derived factor (PEDF) is a potent inhibitor of vascular endothelial grow

92 Pigment Epithelium Derived Factor (PEDF) is a secreted factor that has broad biological act

93 Pigment epithelium-derived factor (PEDF) is a serine protease inhibitor (serpin) protein wi

94 Pigment epithelium-derived factor (PEDF) is a serine proteinase inhibitor with antiangiogen

95 Pigment epithelium-derived factor (PEDF) is a serpin with antiangiogenic properties.

96 cytokine pigment epithelium-derived factor (PEDF) is downregulated in resected human brain metastase

97 Pigment epithelium-derived factor (PEDF) is important for maintaining the normal extracellu

98 Pigment epithelium-derived factor (PEDF) is known to be an angiogenesis suppressor and to h

99 o measure pigment epithelium-derived factor (PEDF) levels from conditioned media.

100 ngiogenic pigment epithelium derived factor (PEDF) may cause choroidal neovascularization (CNV), a ke

101 ngiogenic pigment epithelium derived factor (PEDF) may cause choroidal neovascularization (CNV), key

102 endent on pigment epithelium-derived factor (PEDF) on the outer surface of exosomes.

103 to either pigment epithelial derived factor (PEDF) or an AAV2 vector containing a PS1 gene driven by

104 ated that pigment epithelium-derived factor (PEDF) plus docosahexaenoic acid (DHA) promotes corneal n

105 molecule, pigment epithelium-derived factor (PEDF) plus docosahexaenoic acid (DHA), has been shown to

106 VEGF) and pigment epithelium-derived factor (PEDF) protein, and mRNA levels were analyzed by Western

107 ffects of pigment epithelium-derived factor (PEDF) require interactions between an as of a yet undefi

108 retion of pigment epithelium-derived factor (PEDF) via activation of signal transducer and activator

109 retion of pigment-epithelium-derived factor (PEDF) was directed apically in both cultures, but fhRPE

110 I-1), and pigment epithelium-derived factor (PEDF) were measured by immunoassay at baseline and 12 mo

111 pathway, pigment epithelium-derived factor (PEDF), a multifunctional serine proteinase inhibitor.

112 thermore, pigment epithelium-derived factor (PEDF), a secreted glycoprotein known for its anti-tumor

113 acts via pigment epithelium-derived factor (PEDF), an antiangiogenic protein, to regulate retinal pi

114 (IGF-1), pigment epithelial-derived factor (PEDF), and serum monocyte differentiation antigen (CD-14

115 P-1), and pigment epithelium-derived factor (PEDF), as well as by degeneration of the elastic layer.

116 olecules, pigment epithelium-derived factor (PEDF), is a broadly expressed multifunctional member of

117 ns in for pigment epithelium-derived factor (PEDF), the protein product of the SERPINF1 gene, are the

118 Pigment epithelium-derived factor (PEDF), the protein product of the SERPINF1 gene, has bee

119 EGF), and pigment epithelium-derived factor (PEDF).

120 (HGF) and pigment epithelium-derived factor (PEDF).

121 nhibitor, pigment epithelial-derived factor (PEDF).

122 nd -9 and pigment epithelium-derived factor (PEDF).

123 onized by pigment epithelium-derived factor (PEDF).

124 Loss of pigment epithelium-derived factor (PEDF, SERPINF1) in cancer cells is associated with poor

125 er pigment epithelial-derived growth factor (PEDF), together with docosahexaenoic acid (DHA), enhance

126 molecule, pigment epithelium growth factor (PEDF).

127 EGFR3 and pigment epithelium-derived factor [PEDF]) and for quantitative RT-PCR (VEGFR3, PEDF, and CD

128 y secrete pigment epithelium-derived factor, PEDF, which is known to exert anti-angiogenic actions.

129 blasts can be regulated by specific factors, PEDF-directed dependency of murine and human MSCs was as

130 r has shown an elevated binding affinity for PEDF.

131 EDF on retina cells and has determinants for PEDF binding within its L4 ectodomain that are critical

132 we demonstrated that PEDF-R is essential for PEDF-mediated cell survival and antiapoptotic activities

133 DC1 and PLXDC2 as cell-surface receptors for PEDF.

134 8-114 of PEDF contains critical residues for PEDF-R interaction that mediates survival effects, the f

135 Searching for PEDF regulatory mechanisms revealed two occupied conserv

136 Furthermore, PEDF intracerebral infusion enhanced survival and matura

137 Furthermore, PEDF knockdown by small interfering RNA (siRNA) and PEDF

138 lar neovascularization in vivo, we generated PEDF transgenic (PEDF-Tg) mice that ubiquitously express

139 Accordingly, mice with global PEDF knockout were more susceptible to melanoma metastas

140 at normal dermal fibroblasts expressing high PEDF levels attenuated melanoma growth and angiogenesis

141 A long-standing challenge is to reveal how PEDF acts on its target cells and the identities of the

142 DHT) increased PEDF (qPCR) in HTPCs, however PEDF expression in the testis of a non-human primate occ

143 However, PEDF-R polypeptides without the His(203)-Leu(232) region

144 EDF-Tg) mice that ubiquitously express human PEDF driven by the beta-actin promoter.

145 Recombinant human PEDF (rhuPEDF) was cleaved at its serpin-exposed loop by

146 protein was demonstrated to bind immobilized PEDF, and PEDF was shown to bind to immobilized C1q, in

147 Immunoreactive PEDF receptor was detectable in multiple cell types in b

148 In PEDF knockout (KO) mice, total body adiposity was increa

149 Here we characterized the area in PEDF that interacts with PEDF-R to promote photoreceptor

150 a 2.5-fold increase in corneal nerve area in PEDF+DHA-treated animals compared with control animals.

151 irius red staining revealed more fibrosis in PEDF-null versus wild-type pancreas 1 week after pancrea

152 agen I, and thrombospondin-1) were higher in PEDF-null mice at baseline.

153 interfering RNA (siRNA) and PEDF knockout in PEDF(-/-) mice induced activation of Wnt signaling.

154 ammatory factors were significantly lower in PEDF-Tg mice with OIR than in the Wt mice with OIR.

155 nal pigment epithelium of Wt mice but not in PEDF-Tg mice.

156 stically significantly greater reductions in PEDF (-9.20%, -9.90%, respectively, both P < 0.0001) and

157 Inhibition of MMP-2/9 activity increased PEDF and decreased VEGF levels in the apical and basal s

158 Dihydrotestosterone (DHT) increased PEDF (qPCR) in HTPCs, however PEDF expression in the tes

159 ding and experimental pancreatitis increased PEDF expression in wild-type mice.

160 5)) with affinity similar to the full-length PEDF-R (Met(1)-Leu(504)).

161 ed phospholipase activity of the full-length PEDF-R.

162 The factor involved is likely PEDF, as a PEDF-antiserum blocked the repulsing action.

163 e contact with PEDF-null melanoma cells lost PEDF expression and tumor-suppressive properties.

164 production following demyelination, and make PEDF a strong candidate for pharmacological intervention

165 Mechanistically, PEDF sustained GSC self-renewal by Notch1 cleavage, and

166 Mutation of a single amino acid on a 34-mer PEDF peptide increased mineralization of hMSC cultures c

167 In young (19-d-old) PEDF-KO mice, PEDF restoration increased bone volume fraction by 35% a

168 e mice is down-regulation of PVM/M modulated PEDF production.

169 ) in the first intron of the human and mouse PEDF promoter regions, confirmed by binding assays.

170 Notably, PEDF infusion also resulted in an induction of doublecor

171 from 5-day-old mice were treated with NPD1, PEDF+DHA, lipoxin A4 (LXA4), 12- or 15-hydroxyeicosatetr

172 Additional rabbits were treated with NPD1, PEDF+DHA, or vehicle, and corneal sections were stained

173 t the region composed of positions 98-114 of PEDF contains critical residues for PEDF-R interaction t

174 of bone development; however, the ability of PEDF to restore bone mass in a mouse model of OI type VI

175 Dual antitumor/antiangiogenic activities of PEDF suggest that PEDF gene therapy may be considered an

176 The co-administration of PEDF and VEGF-E depleted the amount of VEGF-R2 in the me

177 EDF in transgenic mice and administration of PEDF protein attenuated Wnt signaling induced by retinal

178 tance were evaluated after administration of PEDF, placenta growth factor (VEGF-R1 agonist), and VEGF

179 In addition, binding of PEDF to LRP6 blocked Wnt ligand-induced LRP6-Frizzled re

180 We demonstrate that delivery of PEDF to the damaged ear ameliorates hearing loss by rest

181 Therefore, the inhibitory effect of PEDF appears to be mediated via the processing of VEGF-R

182 or the survival and antiapoptotic effects of PEDF on retina cells and has determinants for PEDF bindi

183 Here, they examined the effects of PEDF polypeptide fragments on vessel sprouting and on ch

184 nction by blocking the protective effects of PEDF to prevent VEGF from driving the dry to wet AMD tra

185 Notably, the suppressive effects of PEDF were not only rapid but independent of the effects

186 agonists prevented the inhibitory effects of PEDF.

187 In human PanIN lesions, co-expression of PEDF and SOD2 was observed in the majority of early PanI

188 rized hES-RPE showed prominent expression of PEDF in apical cytoplasm and a marked increase in secret

189 ohistochemistry, we determined expression of PEDF in common and dysplastic melanocytic nevi, melanoma

190 Inhibition of PEDF diminished GSC self-renewal and increased survival

191 es either received intravitreal injection of PEDF, DAPT (a gamma-secretase inhibitor) or PEDF + DAPT

192 The physical interaction of PEDF with LRP6 was confirmed by a coprecipitation assay,

193 zation, hES-RPE cells secrete high levels of PEDF that can support RPC survival.

194 hermore, a subpopulation with high levels of PEDF was capable of infiltration along corpus callosum.

195 ived tumors expressed markedly low levels of PEDF.

196 These findings suggest that loss of PEDF expression promotes early invasive melanoma growth.

197 creatic fibrosis is dependent on the loss of PEDF.

198 Both overexpression of PEDF in transgenic mice and administration of PEDF prote

199 these results suggest that overexpression of PEDF inhibits retinal inflammation and neovascularizatio

200 gates whether constitutive overexpression of PEDF inhibits the growth and hepatic micrometastasis of

201 Furthermore, preincubation of PEDF with P1 and E5b peptides blocked the PEDF.PEDF-R-me

202 o investigate the spatiotemporal presence of PEDF and PEDFR in the adult mouse brain, and to determin

203 Finally, expression profiling of PEDF-depleted fibroblasts revealed induction of IL8, SER

204 -R interacts with the neurotrophic region of PEDF (44-mer, positions 78-121).

205 and CNV resides within the 34-mer region of PEDF.

206 decreased VEGF expression and restoration of PEDF levels.

207 ether, these data indicate the novel role of PEDF as a key regulator of GSC and suggest clinical impl

208 l for further investigation into the role of PEDF in inflammatory processes in the joint, which, in c

209 oplasm and a marked increase in secretion of PEDF into the medium compared with nonpolarized culture.

210 However, the PEDF binding site of PEDF-R and its involvement in survival activity have not

211 es suggested that the ligand binding site of PEDF-R interacts with the neurotrophic region of PEDF (4

212 bone volume/total volume by 52% in 6-mo-old PEDF-KO mice but not in wild-type mice.

213 In young (19-d-old) PEDF-KO mice, PEDF restoration increased bone volume fra

214 direct transcriptional influence of MITF on PEDF, establishing the PEDF gene (SERPINF1) as a MITF ta

215 biologically relevant ligand-binding site on PEDF-R.

216 PEDF, DAPT (a gamma-secretase inhibitor) or PEDF + DAPT at the time of laser injury, or AAV2 infecti

217 noculated with constitutively overexpressing PEDF melanoma cells.

218 Endogenous overexpressing PEDF melanoma cells lost the ability to migrate and form

219 Pancreatic PEDF expression was assessed in wild-type mice fed eithe

220 hat PEDF binds and stimulates PEDF receptor (PEDF-R), a transmembrane phospholipase.

221 a distinct ectodomain on the PEDF receptor (PEDF-R).

222 spholipase A2 activity of the PEDF-receptor (PEDF-R) leading to the release of DHA; this free DHA led

223 When immobilized, recombinant PEDF expressed in eukaryotic cells activated the classic

224 ed oligodendrotrophic effects of recombinant PEDF on the adult SVZ and corpus callosum, demonstrate i

225 mice, we found that addition of recombinant PEDF to the medium enhanced expressions of oligodendrogl

226 d the expression of CAF markers and restored PEDF expression.

227 effects, the findings reveal distinct small PEDF fragments with neurotrophic effects on photorecepto

228 says were performed in constitutively stable PEDF-overexpressing cells and transduced lentiviral vect

229 usly reported that PEDF binds and stimulates PEDF receptor (PEDF-R), a transmembrane phospholipase.

230 metastasis, but the contribution of stromal PEDF to cancer evolution is poorly understood.

231 isms by which melanoma cells silence stromal PEDF to promote malignancy.

232 In this study, PEDF delivery increased trabecular bone volume/total vol

233 f the PEDFR labeling pattern was higher than PEDF and included some cortical and subventricular areas

234 d activation of the uPA/uPAR system and that PEDF-mediated inhibition of the VEGF-induced increase in

235 We first confirmed that PEDF + DHA increased nerve regeneration in the mouse cor

236 Collectively, our results demonstrate that PEDF maintains tumor-suppressive functions in fibroblast

237 Our group and others have demonstrated that PEDF directs human mesenchymal stem cell (hMSC) commitme

238 Binding assays demonstrated that PEDF-R bound the 44-mer peptide.

239 PEDF-R in retina cells, we demonstrated that PEDF-R is essential for PEDF-mediated cell survival and

240 It has been described that PEDF plays an important role in neuroprotection and data

241 Our findings establish that PEDF-R is required for the survival and antiapoptotic ef

242 These findings indicate that PEDF acts as a compensatory antifibrotic cytokine in pan

243 These results indicate that PEDF counters Wnt signaling to allow for osteoblast diff

244 These results indicate that PEDF regulates autophagy through coordinate Wnt signalin

245 We have previously reported that PEDF binds and stimulates PEDF receptor (PEDF-R), a tran

246 y a coprecipitation assay, which showed that PEDF bound to LRP6 at the E1E2 domain.

247 Previously, the authors showed that PEDF injected into the subconjunctiva reaches the choroi

248 g synthetic peptides spanning L4 showed that PEDF selectively bound E5b (Ile(193)-Leu(232)) and P1 (T

249 Cell surface labeling showed that PEDF-R is present in the plasma membranes of retina cell

250 Recent studies have also shown that PEDF enhances renewal of adult subventricular zone (SVZ)

251 tiangiogenic activities of PEDF suggest that PEDF gene therapy may be considered an approach for the

252 These results suggest that PEDF is an endogenous antagonist of LRP6, and blocking W

253 Our experiments suggest that PEDF receptors form homooligomers under basal conditions

254 The PEDF-knockout (KO) mouse captures crucial elements of th

255 The PEDF-R inhibitor, atglistatin, blocked all of these chan

256 The PEDF-Tg mice provide a useful model for studying the rol

257 The PEDF-Tg mice under normal conditions did not show any ab

258 of PEDF with P1 and E5b peptides blocked the PEDF.PEDF-R-mediated retina cell survival activity, impl

259 PEDF bound the PEDF-R ectodomain L4 (Leu(159)-Met(325)) with affinity s

260 the adult mouse brain, and to determine the PEDF blood level in mouse and human.

261 In conclusion, we determined the PEDF-mediated events responsible for VEGFR1 signaling an

262 influence of MITF on PEDF, establishing the PEDF gene (SERPINF1) as a MITF target in melanocytes and

263 Moreover, peptide P1 from the PEDF-R ectodomain had affinity for the 44-mer and a shor

264 provides a mechanistic insight into how the PEDF null state results in OI type VI.

265 However, the PEDF binding site of PEDF-R and its involvement in survi

266 d at 1 week and was four times higher in the PEDF+DHA-treated group than in the controls.

267 he CNV area was significantly smaller in the PEDF-Tg mice than in the Wt mice.

268 ithout the His(203)-Leu(232) region lost the PEDF affinity that stimulated their enzymatic activity.

269 Interrogation of the PEDF sequence identified a conserved motif found in othe

270 tivates the phospholipase A2 activity of the PEDF-receptor (PEDF-R) leading to the release of DHA; th

271 ned region with a distinct ectodomain on the PEDF receptor (PEDF-R).

272 Thus PEDF could be involved in the establishment of the avasc

273 l activity, implying that peptide binding to PEDF excluded ligand-receptor interactions on the cell s

274 cells were infected with AAV2 or exposed to PEDF in the presence or absence of VEGF and in vitro ang

275 The effect was similar to PEDF+DHA-treated animals.

276 NT increased VEGF-to-PEDF ratio in RPE through nAchR in vitro and in vivo whi

277 NT increased the VEGF-to-PEDF ratio in the RPE through nAchR in vitro and in vivo

278 ation in vivo, we generated PEDF transgenic (PEDF-Tg) mice that ubiquitously express human PEDF drive

279 4 (Met(1)-Leu(232)) and internally truncated PEDF-R and PEDF-R4 (DeltaHis(203)-Leu(232)) retained pho

280 Recombinant C-terminal truncated PEDF-R4 (Met(1)-Leu(232)) and internally truncated PEDF-

281 ly associated with reduced circulating VEGF, PEDF, and PAI-1, and could provide incentive for reducin

282 xidative stress; complement activation; VEGF/PEDF ratio; and MMP activity.

283 [PEDF]) and for quantitative RT-PCR (VEGFR3, PEDF, and CD45).

284 Thus testicular peritubular cells, via PEDF, may prevent vascularization of human seminiferous

285 d in a murine model of PanIN formation where PEDF was deleted.

286 of early PanIN lesions (47/50, 94%), whereas PEDF and SOD2 immunolocalization in high-grade human Pan

287 oma growth and angiogenesis in vivo, whereas PEDF-depleted fibroblasts exerted tumor-promoting effect

288 To investigate whether PEDF overexpression has an impact on ocular neovasculari

289 udy was to understand the mechanism by which PEDF blocks VEGF-induced increases in vascular permeabil

290 rate that the human diseases associated with PEDF reflect its ability to modulate MSC differentiation

291 hat normal fibroblasts in close contact with PEDF-null melanoma cells lost PEDF expression and tumor-

292 MITF positively correlated with PEDF expression in invasive melanomas (P = 0.0003).

293 the right eye and treated in both eyes with PEDF+DHA for 2 weeks, there was a significant increase i

294 re compared between the mice inoculated with PEDF-overexpressing tumor cells and those mice with the

295 terized the area in PEDF that interacts with PEDF-R to promote photoreceptor survival.

296 ntiation pattern analogous to that seen with PEDF.

297 prisingly, corneal injury and treatment with PEDF + DHA induced transcription of neuropeptide y (npy)

298 Treatment with PEDF activates the phospholipase A2 activity of the PEDF

299 results suggest that topical treatment with PEDF+DHA promotes corneal nerve regeneration and wound h

300 observed in rabbits treated for 8 weeks with PEDF+DHA.