ライフサイエンスコーパス: PH domain

1 ith PI(3,4)P2 on the plasma membrane via its PH domain.

2 s of the full-length protein compared to its PH domain.

3 dient was dependent on its PI(3,4)P2-binding PH domain.

4 exhibited strong binding affinities to GAB1 PH domain.

5 ily as the most likely origin of the kindlin PH domain.

6 Instead it is negatively controlled by the PH domain.

7 inositol 4,5-bisphosphate (PIP2) through the PH domain.

8 e autoinhibitory elements in addition to the PH domain.

9 nge of its interaction partner from Ras to a PH domain.

10 h nuclear localization-dependent on the Vav3 PH domain.

11 ization is an important function of the Vav3 PH domain.

12 h homology to Ras superfamily proteins and a PH domain.

13 y N-terminal to the first beta-strand of the PH domain.

14 tes with PtdIns(4)P for association with the PH domain.

15 ositions near the beta3/beta4 loops of their PH domains.

16 d by overexpression of five of the RhoGEF DH-PH domains.

17 mbrane insertion of its pleckstrin homology (PH) domain.

18 domain with Akt at the pleckstrin homology (PH) domain.

19 targeting site-2 of the pleckstrin homology (PH) domain.

20 f these affect the formation of a functional PHD domain.

21 fy the mechanism of KDM2A played by its CXXC-PHD domain.

22 one-binding affinity by interacting with the PHD domain.

23 lighting the multifunctional nature of ATXR5 PHD domain.

24 nding motifs, SET methyltransferase, HMG and PHD domains.

25 Arf GAP) with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) establishes a connection between the

26 )/ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)-dependent manner.

27 in, ankyrin repeat, and pleckstrin homology (PH) domain 1 (ASAP1) is a multidomain GTPase-activating

28 tein [ArfGAP] with dual pleckstrin homology [PH] domains 1) has been suggested to control dendrite br

29 ding protein-like domain, Ankyrin repeat and PH domain 2) isoform 2 is considered a proto-oncogene, b

30 d that ArfGAP with dual pleckstrin homology (PH) domains 2 (ADAP2), gamma-interferon-inducible lysoso

31 ociating (RA) domain, a pleckstrin-homology (PH) domain, a family-specific BPS (between PH and SH2) r

32 thesis that binding of activated RhoA to the PH domains acts as a positive feedback mechanism.

33 e by site-directed mutagenesis increases the PH domain affinity for Ins(3,4,5)P(3) as measured by sur

34 ast, unmyristoylated HIV-1 MA, RSV MA, and a PH domain all preferred to interact with free PIP2.

35 Overexpression of these PH domains alone can block RhoA-dependent signaling in c

36 We showed previously that the RA and PH domains, along with the BPS region and SH2 domain, ar

37 GAC PH domain also binds to phosphatidic acid, a lipid media

38 that reduced ARF1 N-terminal binding to the PH domain also reduced the effect of ASAP1 on cellular a

39 ptor with neurofibromin Pleckstrin Homology (PH) domain also inhibits receptor constitutive activity,

40 ependently bound to a protein containing the PH domain and a 17-amino acid-long interdomain linker im

41 BAR (Bin-amphiphysin-Rvs) domain linked to a PH domain and a C-terminal phosphotyrosine-binding domai

42 mall molecules that interact with the P-Rex1 PH domain and block binding of and activation by PIP(3)

43 molecule, PH-687, that directly targets the PH domain and disrupts Mcl-1/Akt binding, leading to sup

44 localization to the plasma membrane via the PH domain and implicate this mechanism in the inhibition

45 her form a positively charged surface on the PH domain and interact with the negatively charged headg

46 t is known that the interactions between the PH domain and kinase domain (KD) are important for maint

47 or protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) signaling endosom

48 some 10 (PTEN) overexpression down-regulated PH domain and leucine-rich repeat phosphatase (PHLPP) an

49 in the degradation of two splice variants of PH domain and Leucine-rich repeat Protein Phosphatase 1

50 ion via inhibition of the translation of the PH domain and leucine-rich repeat protein phosphatases 1

51 terically regulated by PIP(2) binding to the PH domain and that activity depends on the interdomain l

52 Thus, the PH domain and the interdomain linker of Brag2 may be tar

53 the N-terminal extension of ARF1 to ASAP1's PH domain and thereby regulates its GAP activity.

54 ort high-resolution structures of the DH and PH domains and characterize conformations of the DH-PH u

55 termined the crystal structure of the RA and PH domains and found that, despite an intervening linker

56 alpha(s) interacted with isolated PRG DH and PH domains and their linker.

57 structure consists of a Pleckstrin Homology (PH) domain and a Phosphotyrosine Binding (PTB) domain.

58 we have focused on its pleckstrin homology (PH) domain and find that deletion of this domain elimina

59 wed that the PLCepsilon pleckstrin homology (PH) domain and first two EF hands (EF1/2) are required f

60 between its N-terminal pleckstrin homology (PH) domain and kinase domain, which is relieved by C-tai

61 Pleckstrin homology (PH) domain and leucine-rich repeat protein phosphatase 1

62 M2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protei

63 ded PHD domain (ePHD(6)) involving the sixth PHD domain and its preceding zinc finger in MLL3 and MLL

64 daptor Protein, Phosphotyrosine Interaction, PH domain, and leucine zipper containing 1 (APPL1) that

65 Brag2 contains a pleckstrin homology (PH) domain, and its nucleotide exchange activity is stim

66 hotyrosine interaction, pleckstrin homology (PH) domain, and leucine zipper-containing protein)) are

67 domain, Fbxl10 consists of a CxxC domain, a PHD domain, and an Fbox domain.

68 Specific residues within the PH domain are responsible for both membrane binding and

69 PH domains are often involved in membrane localization o

70 Pleckstrin homology (PH) domains are lipid-binding modules present in periphe

71 we report that acidic residues in the BAZ2B PHD domain are essential for H3 binding and that BAZ2B P

72 identify a hydrophobic face of the PLC-beta PH domain as the Gbetagamma binding interface.

73 fic small-molecule targeting PIP3 binding by PH domains as potential anticancer agents that can simul

74 tor MS1 (MALE STERILITY 1), which contains a PHD domain associated with chromatin re-organization.

75 lin 3-IPRR, has a four-residue insert in the PH domain at a critical site that influences phosphoinos

76 ated the search and bind process in the GRP1 PH domain at the molecular scale.

77 revealed that the START domain binds to the PH domain at the same site for PtdIns(4)P-binding, sugge

78 ity of DMIB for PIP(2)/PIP(3) similar to the PH domain-based specificity of other class I myosins.

79 onstrate that PITs inhibit PIP3/ARNO or GRP1 PH domain binding and membrane localization, resulting i

80 PH domain binding leads to local clustering of PIP molec

81 kt phosphatase, through pleckstrin homology (PH) domain binding.

82 Although lipid overlay assays suggested the PH domain binds inositol monophosphates, surface plasmon

83 nine nucleotide exchange factor, whereas the PH domain binds to various phosphatidylinositol-phosphat

84 tylation while a double pleckstrin-homology (PH) domain binds the K56-containing region of H3.

85 as association (RA) and pleckstrin homology (PH) domains, both of which were required for lymphocyte

86 The PH domain bound phosphoinositol 4,5-bisphosphate (PI(4,5

87 iled molecular characterization of tandem RA-PH domains bound to a small GTPase and provides insights

88 e of Pak1/2, we found that ArhGAP15, via its PH domain, bound to these kinases.

89 Lipid binding is mediated by the PH domain, but is further enhanced by a central helical

90 Hence, the PH domain, but not the DH domain, plays an important rol

91 ealed that phosphoinositide recognition by a PH domain can be switched through its phosphorylation.

92 We show that the isolated PH domain can compete with full-length PLC-beta3 for bin

93 PH domains can be grouped by the relative strength of th

94 Furthermore, PH domains carrying mutations identified in NF1 patients

95 arbours a non-canonical pleckstrin homology (PH) domain connected to a 16-leucine-rich repeat domain.

96 Ventricular zone expressed PH domain-containing 1 (VEPH1) is the mammalian ortholog

97 helial specific gene named FYVE, RhoGEF, and PH domain-containing 5 (FGD5) that plays a crucial role

98 that Arf-GAP with coil-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) functions as an a

99 Furthermore, we identify a tandem PH domain-containing protein, Opy1, as a novel Mss4-inte

100 lso suggest that additional conserved tandem PH domain-containing proteins may play important roles i

101 3,4)P(2,) which regulates the recruitment of PH domain-containing scaffolds such as lamellipodin to i

102 we identify an acylated pleckstrin-homology (PH) domain-containing protein (APH) on the microneme sur

103 t that mutations in the pleckstrin homology (PH) domain-containing protein AtPH1 rescue the iron-defi

104 ophila melted encodes a pleckstrin homology (PH) domain-containing protein that enables normal tissue

105 conserved armadillo and pleckstrin homology (PH) domain-containing protein, termed glideosome-associa

106 ppressor of Ras 1) is a pleckstrin homology (PH) domain-containing scaffold protein that increases th

107 nd consequently recruit pleckstrin homology (PH) domain-containing signaling proteins.

108 chromodomain-containing Eaf3 subunit and the PHD domain-containing Rco1 subunit to recognize nucleoso

109 taneous binding of multiple PIP molecules by PH domains contributes to high-affinity membrane interac

110 linker or the C-terminal alpha-helix of the PH domain decreased [2-17]ARF1 binding and GAP activity.

111 of a serine-rich motif immediately after the PH domain decreases both PtdIns(4)P binding and ceramide

112 ed at the C-terminal region encompassing the PH domain, decreases SMAD2 retention at TbetaRI and enha

113 In contrast, PKD1-DeltaPH (a PH domain deletion mutant) is recovered as a constitutiv

114 g a two-amino acid deletion within the SH2B1 PH domain (DeltaP317, R318 [DeltaPR]) were studied.

115 protected from drug induced cell death in a PH-domain dependent manner.

116 However, the molecular mechanisms underlying PH domain-dependent membrane fission remain obscure.

117 oactivates AR in a Vav3 pleckstrin homology (PH) domain-dependent but GEF-independent manner.

118 The binding of Mcl-1/PH domain disrupted intramolecular PH/KD interactions to

119 P3 search process, before and after the GRP1 PH domain docks with the PIP3 lipid.

120 techniques, we demonstrate that the isolated PH domain does not act to bend membranes but instead sen

121 Our results indicate that tilt of the PH domain drives deformation and fragmentation of the ol

122 Third, we show that ATXR5 PHD domain employs a narrow binding pocket to selectivel

123 protein marks by PCGEM1-recruited pygopus 2 PHD domain enhances selective looping of androgen-recept

124 Here we show that an extended PHD domain (ePHD(6)) involving the sixth PHD domain and

125 The PHD domain exerts a dual function in binding H3K4me3 and

126 ns in the number of PIPs associated with the PH domain exhibit 1/f noise.

127 inhibits receptor constitutive activity, and PH domain expression rescues 5-HT6 receptor-operated cAM

128 a confirming the importance of the kindlin-1 PH domain for integrin activation and its x-ray crystal

129 allel dimerization, properly positioning the PH domains for simultaneous membrane interaction.

130 ous studies have reported specificity of the PHD domain for the unmodified N terminus of histone H3 a

131 The structure reveals that the Grb14 RA and PH domains form an integrated structural unit capable of

132 hosphorylation of PTEN to release the P-REX2 PH domain from its neighboring diffuse B-cell lymphoma h

133 he Warts kinase of the Hippo pathway and the PH-domain growth regulator Melted regulates the choice b

134 We show that the kindlin 3 PH domain has binding affinity for phosphoinositide PI(3

135 (Arfgap with Coil-coil, Ankryin repeat, and PH domain I) into an unusual lattice structure that also

136 In cells, deletion of the PH domain impairs leading edge localization.

137 ed that the linker between the sec7d and the PH domain in Brag2 may directly contact Arf.

138 s to characterize structural features of the PH domain in its autoinhibited and activated states.

139 Therefore, intrinsic movement of the PH domain in PLC-beta modulates Gbetagamma access to its

140 es rich in PtdIns, suggesting a role for the PH domain in the biological activity of Cnk1.

141 the two membrane binding states of the GRP1 PH domain in the PIP3 search process, before and after t

142 ture at 2.4-A resolution of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras (G12V).

143 fragment of P-Rex1 and interacts with the DH/PH domains in solution.

144 of the (H3-H4)(2) tetramer while two double PH domains in the Rtt106 dimer interact with each of the

145 our understanding of membrane recognition by PH domains in vivo.

146 actions between both the peckstrin homology (PH) domain in Lpd and phosphatidylinositol (3,4)-bisphos

147 tal structure defines a pleckstrin homology (PH) domain in REC114 and its direct intermolecular conta

148 These ArfGEFs carry a pleckstrin homology (PH) domain in tandem with their catalytic Sec7 domain, w

149 PHLPP1, as its binding affinities for other PH domains, including those of ILK and PDK1, were an ord

150 resents the discovery of first-in-class GAB1 PH domain inhibitors with potential for targeted breast

151 The BAR domain adjacent to the PH domain instead interacts with the BAR domains of neig

152 ecular dynamics (MD) simulations of the GRP1 PH domain interacting with phosphatidylinositol (3,4,5)-

153 nists (PITs) that block pleckstrin homology (PH) domain interaction, including activation of Akt, and

154 We investigated the molecular basis of FAPP1-PH domain interactions with PI4P bilayers in liposome se

155 tate and found that the pleckstrin homology (PH) domain interacts with the Dbl homology (DH) domain i

156 region comprising three pleckstrin homology (PH) domains interacts with the N-terminal region compris

157 toinhibitory elements in grooves at the Arf6/PH domain interface.

158 NA-binding ARID domain and a histone-binding PHD domain into the Jumonji domain, which separates the

159 us, the correct functioning of the kindlin 3 PH domain is central to the role that kindlin 3 performs

160 in homology (PH) domain, suggesting that the PH domain is essential for SH2B1's function.

161 The specific PIP3 lipid docking of GRP1 PH domain is essential to protein cellular function and

162 The initial encounter of the Dok7 PH domain is followed by formation of additional interac

163 buted, but it remains to be seen whether the PH domain is involved in either process independent of d

164 he phosphate binding pocket in the kindlin-1 PH domain is more occluded than in kindlins-2 and -3 due

165 st the physiological ligand of the kindlin-1 PH domain is most likely not an inositol phosphate but a

166 on membranes and that, similar to BRAGs, its PH domain is not autoinhibitory and strongly potentiates

167 However, in the context of ORP4L, the PH domain is required for normal organization of the vim

168 omology (PH) domain, we demonstrate that the PH domain is the minimal Gbetagamma binding region in PL

169 al studies suggest that the key role of this PH domain is to mediate the Ste5-Ste11 interaction.

170 The pleckstrin homology (PH) domain is one of the most widespread, binding specif

171 The pleckstrin homology (PH) domain is well known for its phospholipid targeting

172 Intramolecular pleckstrin homology (PH) domain-kinase domain (KD) interactions are important

173 teraction by competitive binding of the Akt2-PH domain led to reduced cell viability and invasion.

174 We show that the phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP

175 In this study, we demonstrated that PH domain leucine-rich repeat protein phosphatase (PHLPP

176 we report that deletion of the gene encoding PH domain Leucine-rich repeat Protein Phosphatase 1 (PHL

177 PHLPP1 (PH domain leucine-rich repeat protein phosphatase 1) is

178 membrane co-localization of Akt and PHLPP1 (PH domain leucine-rich repeat protein phosphatase isofor

179 PH domain leucine-rich-repeats protein phosphatase (PHLP

180 Akt phosphatase PHLPP1 (pleckstrin homology (PH) domain leucine-rich repeat protein phosphatase) to A

181 ve the C1 domain (but not the autoinhibitory PH domain) limit maximal PKD1 activity toward physiologi

182 Pleckstrin homology (PH) domains mediate protein-membrane interactions by bin

183 ensory function, disruption of the predicted PH domain-mediated interactions with Gbetagamma and phos

184 esults indicate a functional role for P-REX2 PH-domain-mediated inhibition of PTEN in regulating insu

185 utational analysis supports the bifunctional PH domain mediation of DH-RhoA interactions and explains

186 ate, GTP-insensitive "electrostatic mode" of PH domain-membrane interactions that retains dynamin on

187 Blocking PH domain motion in PLC-beta by cross-linking it to the

188 tructures and an open conformation where the PH domain moves away from the EF hands.

189 PH domains must locate specific PIPs in the presence of

190 PH-domain mutants alter subcellular localization and res

191 Now we show that PH domains occur in all Dictyostelium myosin 1s and that

192 Using the Mcl-1-binding PH domain of Akt as a docking site, we identified a nove

193 phenylquinolin-2(1H)-one interacted with the PH domain of Akt, apparently inducing a conformation tha

194 SC79 specifically binds to the PH domain of Akt.

195 between an internal domain of AEG-1 and the PH domain of Akt2, a major driver in GBM.

196 The effect of PIP(2) required the PH domain of Brag2 and the N terminus of Arf and was lar

197 The unique tandem C2-PH domain of CAPS may serve as a PI(4,5)P2-triggered swi

198 pid transfer protein CERT that relied on the PH domain of CERT.

199 The PH domain of Cnk1 bound with greater affinity to PtdIns(

200 Thus, the PH domain of Cnk1 is a druggable target whose inhibition

201 mpound PHT-7.3 that bound selectively to the PH domain of Cnk1, preventing plasma membrane colocaliza

202 ly block oncogenic KRas activity through the PH domain of Cnk1, which reduces its cell membrane bindi

203 Our simulations indicate that the PH domain of Dok7 associates with membranes containing p

204 als that fit into a surface groove of the DH-PH domain of LARG, a G-protein-regulated Rho GEF involve

205 The PH domain of ORP4L also binds phosphatidylinositol 4-pho

206 These results suggest that the PH domain of P-Rex1 is a tractable drug target and that

207 d domain) with a subsequent insertion of the PH domain of separate origin.

208 not interact with Ras, but instead binds the PH domain of the Ste5 scaffold.

209 ify a potential role for the auto-inhibitory PH domain of Tiam1 in regulating Tiam1 function at these

210 The GLD and PH domains of AGAP1 bound the motor domain of Kif2A.

211 AGAP1, and a protein composed of the GLD and PH domains of AGAP1 increased ATPase activity of Kif2A.

212 Like 3, 20 preferentially recognized the PH domains of Akt and PHLPP1, as its binding affinities

213 ed pool of PI4P as well as the expression of PH domains of Golgi proteins that specifically recognize

214 Y16 binds to the junction site of the DH-PH domains of LARG with a approximately 80 nM K(d) and s

215 , mutation of the BH site located within the PH domains of PIP3-specific Myo1D and Myo1F completely e

216 Thus, the RA-PH domains of RIAM function as a proximity detector for

217 m4p interacted with the pleckstrin homology (PH) domain of Cdc24p, which functions in an autoinhibito

218 over, we identified the pleckstrin homology (PH) domain of G protein-coupled receptor kinase 2 (Gprk2

219 y, we aim to target the pleckstrin homology (PH) domain of GAB1 for cancer treatment.

220 The pleckstrin homology (PH) domain of Lbc is located at the C-terminal end of th

221 inding of PIP(3) to the pleckstrin homology (PH) domain of P-Rex1 is required for its activation in c

222 The pleckstrin homology (PH) domain of P-REX2 inhibits PTEN by interacting with t

223 RhoA also binds to the pleckstrin homology (PH) domain of PDZRhoGEF.

224 ng proteins such as the pleckstrin homology (PH) domain of phospholipase Cdelta1.

225 The pleckstrin homology (PH) domain of the general receptor of phosphoinositides

226 osphate (PIP(3)) to the Pleckstrin Homology (PH) domain of the Tec family protein tyrosine kinase, In

227 e Dbl-homology (DH) and Pleckstrin-homology (PH) domains of Bcr-Abl p210, which constitute the only s

228 tive recruitment of the pleckstrin homology (PH) domains of FAPP proteins to the trans-Golgi network.

229 h the Dbl homology (DH)/pleckstrin homology (PH) domains of SOS, bringing GDP-Ras to the proximity of

230 aging of InsP7 leads to translocation of the PH-domain of Akt, an important signalling-node kinase in

231 posing pathomechanism due to variants in the PH-domain of WDFY3 leads to microcephaly.

232 and the only missense-variant located in the PH-domain of WDFY3.

233 By purifying the JmjC and the PHD domain of Fbxl10 and using different approaches we w

234 Recently, the PHD domains of MLL3/4 have been reported to recruit the

235 ranslational and rotational diffusion of the PH domain on the lipid membrane surface exhibit transien

236 lization and anomalous dynamics of the DAPP1 PH domain on the surface of a PIP-containing lipid bilay

237 binding may occur through PIP3/PIP2-specific PH domains or nonspecific ionic interactions involving b

238 ce energy transfer approaches to distinguish PH-domain orientation from proximity at the membrane sur

239 and a variant without a pleckstrin homology (PH) domain (ORP4S) bind 25-hydroxycholesterol and extrac

240 d the reappearance of the PI(4,5)P2-specific PH domain PH-phospholipase C delta-EGFP at the PM after

241 or protein containing a pleckstrin homology (PH) domain, phosphotyrosine-binding (PTB) domain, and le

242 or protein containing a pleckstrin-homology (PH) domain, phosphotyrosine-binding (PTB) domain, and le

243 domain (MTD) families, such as C1-, C2- and PH domains, play a key role in signal transduction and m

244 These studies demonstrate that the PH domain plays a crucial role in how SH2B1 controls ene

245 In vivo, ARAP3 PH domain point mutant bone marrow chimeras exhibit redu

246 ARAP3 PH domain point mutant neutrophils are characterized by

247 utrophils from an ARAP3 pleckstrin homology (PH) domain point mutation knock-in mouse (R302, 303A), i

248 A PH-domain probe revealed that the regulatory lipid phosp

249 (Arfgap with Coil coil, Ankyrin repeat, and PH domain protein 1) contains a BAR domain.

250 This work identifies a PH domain protein as a regulator of plant metal transpor

251 ArfGAP with coiled-coil, ankyrin repeat, and PH domains protein 1) is an ARF6 GAP that also acts as a

252 porter localization, providing evidence that PH domain proteins may be effectors of PI3P for protein

253 ery, but involving PI3-kinase and downstream PH domain proteins, CRAC and PhdA.

254 possessing independent GEF activity and the PH domain providing a broad seat for RhoA-GTP docking ra

255 demonstrate that deletion or mutation of the PHD domain reduces the catalytic efficiency (kcat/Km of

256 e-bound dynamin spirals, indicating that the PH domain regulates membrane fission through the control

257 ether with the XRCC5/6 complex, and the CXXC-PHD domain regulates the CpG-rich IGFBPL1 promoter.

258 rientation of dynamin's pleckstrin homology (PH) domain relative to the underlying membrane.

259 nding to a proline-tyrosine motif in the Akt-PH domain, SAV1 suppresses Akt activation by blocking Ak

260 allosteric inhibitor MK2206 drives distinct PH domain structural changes compared to baseline autoin

261 2B1 variants lie in the Pleckstrin homology (PH) domain, suggesting that the PH domain is essential f

262 Structural comparison with other PH domains suggests that the phosphate binding pocket in

263 This PH domain surface is not solvent-exposed in crystal stru

264 CERT's N-terminal pleckstrin homology (PH) domain targets it to the Golgi by binding to phospha

265 fy a role for a dynamic short segment in the PH domain that appears to regulate autoinhibition and PD

266 sphorylate Rok near the pleckstrin homology (PH) domain that mediates membrane association.

267 ma membrane through its pleckstrin homology (PH) domain that recognizes phosphatidylinositol 3,4,5-tr

268 (Slm1 and Slm2) contain pleckstrin homology (PH) domains that exhibit specificity for binding phospha

269 Unlike previously characterized CD and PHD domains that bind to their targets with micromolar a

270 ding site and unique peripheral sites of the PH domain, the Arf GTPase and, unexpectedly, the Sec7 do

271 Together with the tethering PH domain, three membrane-associating elements synergist

272 dle is coupled to the structurally dependent PH domain through a helical linker, which reduces its ac

273 that the phosphorylation of Grp1 induces its PH domain to recognize instead phosphatidylinositol 4-ph

274 ude that PIP(2) regulates binding of ASAP1's PH domain to the ARF1 N terminus, which may partially re

275 etween non-PIP anionic lipids attracting the PH domain to the bilayer surface in a favourable orienta

276 We have quantified the binding of the PH domain to the model bilayers by calculating a density

277 nstrate that binding of pleckstrin homology (PH) domains to phosphatidylinositol 4-phosphate (PI4P) i

278 hieved by the binding of the PCL1 N-terminal PHD domain to the C-terminal domain of p53 through two u

279 orientational electrostatic steering of the PH domain towards the PI(3,4,5)P(3)-containing anionic b

280 ng of all ORP4 variants (including a partial PH domain truncation termed ORP4M) in HEK293 and HeLa ce

281 domain in tandem with a Pleckstrin Homology (PH domain) underlies the assembly of ACAP1 (Arfgap with

282 molecular dynamics studies showed that GAB1 PH domain underwent large conformational changes upon li

283 ublished data, demonstrating that pathogenic PH-domain variants can lead to microcephaly via canonica

284 Membrane penetration by the FAPP1-PH domain was mediated by an exposed, conserved hydropho

285 over, truncated Avo1 (lacking its C-terminal PH domain) was still recruited to the PM and supported g

286 olecule at the canonical binding site of the PH domain, we observe additional PIP molecules in contac

287 P(2) with a green fluorescent protein-tagged PH domain, we show that PLCzeta effects minimal loss of

288 or 594-labeled PLC-beta pleckstrin homology (PH) domain, we demonstrate that the PH domain is the min

289 oinositide (PI) binding pleckstrin homology (PH) domain, we focused on genetic interactions between a

290 nt distributions of cationic residues on the PH domain, were observed, involving PIP interactions at

291 PPIs with the expression of nuclear-targeted PH domains, which inhibits recruitment of Ataxia telangi

292 Association of the Dok7 PH domain with PIP lipids is therefore seen as a key ste

293 and long-term correlated interaction of the PH domain with the membrane may contribute to an enhance

294 dscapes for the interactions of 12 different PH domains with membranes containing PIP2 or PIP3, allow

295 the complexities of the interactions of the PH domains with PIP molecules in membranes.

296 ular interaction of the pleckstrin homology (PH) domain with the von Willebrand type A (VWA) domain b

297 mology (PH) domain within Rgc2 and a partial PH domain within Fps1.

298 ain is interrupted by a pleckstrin homology (PH) domain within its F2 subdomain.

299 n involves a tripartite pleckstrin homology (PH) domain within Rgc2 and a partial PH domain within Fp

300 tural and dynamical insights into how DH and PH domains work together in solution to support regulate