ライフサイエンスコーパス: PKC-theta

1 PKC theta and 14-3-3 tau coimmunoprecipitated from Jurka

2 PKC-theta activation of NF-kappaB is mediated through th

3 PKC-theta acts directly or indirectly to stimulate phosp

4 PKC-theta and calcineurin are also partially responsible

5 PKC-theta is thus required for maximum up-regulation of

6 PKC-theta is thus required for promoting FasL expression

7 PKC-theta plays a central role in Teff cell activation a

8 PKC-theta specifically cooperates with calcineurin, and

9 PKC-theta then couples IkappaB kinases (IKKs) to the CBM

10 PKC-theta transduces the signal from the TCR through act

11 PKC-theta(-/-) murine platelets showed significantly low

12 PKC-theta(-/-) T cells had reduced activity of the AKT k

13 PKC-theta-deficient CD4(+) T cells show an impaired prol

14 PKC-theta-mediated activation of Bcl-xL reporter was inh

15 PKC-theta-mediated activation of the Stat3 promoter was

16 also accumulates in the immune synapse in a PKC theta-dependent manner upon antigen recognition by T

17 struct driven by two tandem -180 sites and a PKC-theta construct caused a significant increase in the

18 7 region of the IL-2 promoter/enhancer and a PKC-theta construct caused a similar increase in the rep

19 Th1 cell differentiation, whereas activated PKC-theta reverted only GATA3 transcription factor and t

20 ansfected wild-type or constitutively active PKC theta, as well as by endogenous PKC in ionomycin- an

21 Constitutively active PKC-theta, or WT PKC-theta activated by either PMA or TC

22 In addition, PKC theta-/- T cells failed to up-regulate LFA-1 express

23 In addition, PKC-theta levels were decreased in both membrane and cyt

24 In addition, PKC-theta was associated with Lck in the rafts.

25 In addition, PKC-theta(-/-) T cells displayed resistance to Fas-media

26 SLAM engagement amplified PKC-theta recruitment in wt but not SAP- or Fyn-deficien

27 The interaction between 14-3-3 and PKC theta may represent an important general mechanism f

28 ys confirmed the association of PKC beta and PKC theta with spectrin following its reorganization.

29 rologous cultures combining normal nerve and PKC theta-deficient muscle, as might be expected from th

30 APCs polymerize actin and organize talin and PKC theta normally, forming an immune synapse that is st

31 icient T cells show less efficient talin and PKC theta polarization.

32 s study the potential roles of PKC-alpha and PKC-theta in MDR1 gene transcriptional regulation were e

33 nscription can be regulated by PKC-alpha and PKC-theta.

34 Similarly, calcineurin and PKC-theta specifically synergize to induce transcription

35 iated coordinately by both Akt-dependent and PKC-theta-dependent signaling pathways in primary T cell

36 glycerol content and reduced PKC-epsilon and PKC-theta activity in liver and muscle respectively.

37 ild-type mice was performed in wild-type and PKC-theta-deficient (PKC-theta(-/-)) mice.

38 lycemic clamps did not differ between WT and PKC-theta KO mice following saline infusion.

39 marrow from LysM-GFP(+) (wild-type [WT]) and PKC-theta gene-deficient (Prkcq(-/-)) mice.

40 on of a kinase-deficient mutant or antisense PKC-theta selectively inhibits CD3-CD28 costimulation, b

41 We identified the kinase activity as PKC-theta on the basis of immunodepletion of the moesin

42 or treatment with PMA was found to attenuate PKC theta; and CN synergism.

43 re, all these molecules (spectrin, PKC beta, PKC theta, and receptor for activated C kinase-1) cotran

44 physical and functional interactions between PKC theta, a Ca(2+)-independent PKC enzyme which is expr

45 Preferential cooperation between PKC-theta and calcineurin is observed in Jurkat T cells

46 ognition by T cells and is phosphorylated by PKC theta at Ser-225, which is required for lipid raft t

47 P is phosphorylated in the immune synapse by PKC theta and thereby targeted to lipid rafts to temper

48 reas modulation of suppressive activities by PKC-theta and Dlgh1 signaling pathways are shared.

49 The induction of NF-kappaB by PKC-theta is mediated through the activation of IkappaB

50 er/enhancer in normal T cells is mediated by PKC-theta.

51 hat this site is functional and regulated by PKC-theta.

52 of SHP-1 in NK cells on the S591 residue by PKC-theta promotes the inhibited SHP-1 'folded' state.

53 rtial T-cell activation that failed to cause PKC-theta translocation also failed to cause T-cell prol

54 is article, we show that purified, naive CD4 PKC-theta(-/-) T cells were defective in Th17 differenti

55 In response to TCR stimulation, CD4+ PKC-theta-/- T cells failed to up-regulate Bcl-xL, and u

56 several PKC isoenzymes expressed in T cells, PKC-theta is unique in being rapidly recruited to the si

57 In conclusion, PKC-theta isoform plays a significant role in platelet f

58 In contrast, PKC-theta activated only two MDR1 fragments, -982 and -6

59 In contrast, PKC-theta inactivation prevented fat-induced defects in

60 In contrast, PKC-theta was sequestered away from the Treg immunologic

61 KC-theta activation via Vav-1, which couples PKC-theta to PI3-K and allows it to be phosphorylated.

62 vation in SAP-deficient cells, but decreased PKC-theta recruitment, Bcl-10 phosphorylation, IkappaB-a

63 formed in wild-type and PKC-theta-deficient (PKC-theta(-/-)) mice.

64 ockade enhanced Treg function, demonstrating PKC-theta inhibits Treg-mediated suppression.

65 f MKK4, suggesting that interferon-dependent PKC-theta activation regulates downstream engagement of

66 d that the TCR-derived signaling that drives PKC theta; membrane translocation requires the Src famil

67 Overexpression of either PKC-theta in MCF-7 cells or PKC-epsilon in R6 and NIH 3T

68 Furthermore, knockdown of endogenous PKC-theta expression in Jurkat cells resulted in signifi

69 form of PKC-theta or knockdown of endogenous PKC-theta led to inhibition of Bcl-xL reporter.

70 ing that a SAP/Fyn-mediated pathway enhances PKC-theta/NF-kappaB1 activation and suggesting a role fo

71 PMA, or by CD28 crosslinking, which enhances PKC-theta activation.

72 G vesicle binding experiments with expressed PKC-theta and moesin demonstrate that both bind to vesic

73 Finally, PKC-theta(-/-) mice displayed unstable thrombus formatio

74 n, suggesting the potential compensation for PKC-theta function.

75 nd association with CD28 to be essential for PKC-theta-mediated downstream signaling and the differen

76 JNK activation is an important function for PKC-theta in T-cell activation.

77 the SUMO E3 ligase PIASxbeta as a ligase for PKC-theta.

78 ion, further demonstrating a requirement for PKC-theta in IFN-dependent transcriptional activation.

79 type T cells, suggesting a positive role for PKC-theta in TCR-mediated Ca2+ mobilization.

80 tely absent in antigen-specific T cells from PKC theta-/- mice.

81 itiated NF-kappaB activation was absent from PKC-theta(-/-) mature T lymphocytes, but was intact in t

82 ion, and segregation of TCR-MHC-peptide from PKC-theta-enriched signaling complexes.

83 Furthermore, PKC-theta blockade enhanced Treg function, demonstrating

84 Furthermore, PKC-theta(-/-) T cells had notably lower levels of Stat3

85 Therefore our findings identify PKC-theta as a critical factor for ILC2 activation that

86 duced in the spleens of MOG(35-55)-immunized PKC theta-/- mice as well as in in vitro-stimulated CD4+

87 tion of splenic T lymphocytes from immunized PKC theta-/- mice revealed significantly reduced product

88 Together, these results implicate PKC-theta in one pathway of CD3-CD28 costimulation leadi

89 In PKC-theta-/- mice, peripheral mature T cells, but not de

90 nd diminished joint pathology (p < 0.005) in PKC-theta-deficient compared with wild-type littermates.

91 on of a constitutively active form of AKT in PKC-theta(-/-) T cells restored the ability to inhibit i

92 constitutively active form of calcineurin in PKC-theta-/- Jurkat cells could readily overcome the abo

93 receptor was also significantly decreased in PKC-theta(-/-) mice compared with wild-type littermates.

94 of Vbeta8(+) CD4(+) T cells was defective in PKC-theta(-/-) mice.

95 ingly, the single and combined deficiency in PKC-theta or CARMA-1-the two signal transducers at the N

96 inhibit or promote iTreg differentiation in PKC-theta(-/-) T cells accordingly, indicating that the

97 nificantly increased Th17 differentiation in PKC-theta(-/-) T cells, suggesting that reduced Stat3 le

98 nduced iTreg differentiation was enhanced in PKC-theta(-/-) T cells or wild-type cells treated with a

99 IgG1 and IgG2a anti-collagen II Ab levels in PKC-theta-deficient mice.

100 r Th1 and Th2 differentiation, was normal in PKC-theta(-/-) T cells.

101 sufficient to inhibit apoptosis observed in PKC-theta knockdown cells.

102 L, but not Fas, was significantly reduced in PKC-theta(-/-) mice.

103 Transgenic expression of Bcl-xL in PKC-theta-/- T cells was sufficient to restore cardiac a

104 Expression of catalytically inactive PKC theta; or a dominant-negative mutant of PKC theta; f

105 crosslinking alone was sufficient to induce PKC theta; capping in naive CD4(+) T cells.

106 3-3 tau also inhibited phorbol ester-induced PKC theta translocation from the cytosol to the membrane

107 on TCR stimulation and regulates TCR-induced PKC-theta activation via Vav-1, which couples PKC-theta

108 d the therapeutic implications of inhibiting PKC-theta in Teff cells, to reduce effector function, an

109 ast to adoptive transfer experiments, intact PKC-theta-/- mice displayed delayed, but successful card

110 , introgression of the Bcl2l1 transgene into PKC-theta null mouse failed to rescue NKT cell developme

111 Novel PKC isoform PKC-theta is activated by glycoprotein VI (GPVI) and pro

112 e, we assessed whether one specific isoform, PKC-theta, was expressed in critical CNS regions that re

113 We found that the kinase PKC-theta was sumoylated upon costimulation with antigen

114 Mutation of two kinases (PKC-theta and p21-activated kinase 1 (PAK1)) confirmed c

115 and T cells, is disrupted in T cells lacking PKC theta and in B and T cells lacking Bcl10, a caspase

116 TCR stimulation, peripheral T cells lacking PKC-theta; failed to activate NF-kappaB and AP-1, and to

117 Mice lacking PKC-theta had reduced ILC2 numbers, TH2 cell numbers and

118 Consistently, in murine platelets lacking PKC-theta, platelet aggregation and secretion were also

119 On the other hand, siRNA-mediated PKC-theta inhibition blocked phosphorylation/activation

120 FN-activated STAT pathway, as siRNA-mediated PKC-theta knockdown had no effects on STAT1 phosphorylat

121 Moreover, PKC-theta, like SAP, was required for SLAM-mediated incr

122 ity by overexpression of a dominant-negative PKC-theta mutant also blocked GAS-driven transcription,

123 ulated the expression of Stat3 in WT but not PKC-theta(-/-) T cells.

124 17 differentiation in wild type (WT) but not PKC-theta(-/-) T cells.

125 ) inhibited Ca2+ influx in wild-type but not PKC-theta-/- T cells, suggesting that PKC-theta plays a

126 PKC isoforms, classical PKC alpha and novel PKC theta, have been shown to be enriched in skeletal mu

127 ver, the in vivo consequences of ablation of PKC theta on T cell function in inflammatory autoimmune

128 The presynaptic action of PKC theta implied by this observation is blocked by TTX,

129 pathway in CD3/CD28-stimulated activation of PKC theta and NF-kappaB, and suggest that Vav-1 associat

130 ade and may be mediated by FFA activation of PKC theta.

131 ssion was dramatically reduced in the CNS of PKC theta-/- mice compared with wild-type mice during th

132 n cluster as defined by punctate focusing of PKC theta, phospho-PKC theta, and phospho-ZAP70.

133 ing that 14-3-3 tau inhibits the function of PKC theta not only by preventing its translocation to th

134 These results underscore the importance of PKC theta in the regulation of multiple T cell functions

135 Depletion of Carma1 and inhibition of PKC theta impair accumulation of NF-kappaB complexes in

136 to investigate the potential involvement of PKC theta in the development of experimental autoimmune

137 14-3-3 tau caused increased localization of PKC theta in the particulate fraction in unstimulated ce

138 led to a diffuse pattern of localization of PKC theta; and lymphocyte function-associated antigen-1

139 molecular activation cluster localization of PKC theta; in CD28(+) T cells.

140 Loss of PKC theta expression by induction of "RNA interference"

141 ll activation, a dominant negative mutant of PKC theta decreased the interaction.

142 PKC theta; or a dominant-negative mutant of PKC theta; failed to synergize with CN and did not incre

143 In the absence of PKC-theta, Fas-induced activation of apoptotic molecules

144 gical synapse and central co-accumulation of PKC-theta and CD28.

145 iacylglycerol, with subsequent activation of PKC-theta and inhibition of insulin signaling in muscle.

146 ncentrations, which results in activation of PKC-theta leading to increased IRS-1 Ser(307) phosphoryl

147 Activation of PKC-theta with PMA promoted Th17 differentiation in wild

148 ion did not affect the catalytic activity of PKC-theta but inhibited the association of CD28 with PKC

149 owed a selective increase in the activity of PKC-theta, indicating that the translocated enzyme is ac

150 r, AICD as well as Fas-mediated apoptosis of PKC-theta(-/-) T cells were restored in the presence of

151 Similarly, blocking of PKC-theta kinase activity by overexpression of a dominan

152 moesin kinase activity and copurification of PKC-theta with the enzymic activity.

153 Here we found that the V3 domain of PKC-theta was necessary and sufficient for localization

154 10-R98S cells, in part via downregulation of PKC-theta, with no effect on RPL10-WT cells.

155 entiation, and that Stat3 lies downstream of PKC-theta.

156 Treg differentiation of iTregs downstream of PKC-theta.

157 The enhancing effect of PKC-theta on Ca2+ influx is not only dependent on the st

158 We examined the effects of PKC-theta deficiency on two Th1-dependent models of Ag-i

159 The effects of PKC-theta on IFN-dependent gene transcription were not m

160 Furthermore, ectopic expression of PKC-theta stimulated a reporter gene driven by a mouse B

161 Whereas an inactive form of PKC-theta or knockdown of endogenous PKC-theta led to in

162 Treg freed of PKC-theta-mediated inhibition can function in the presen

163 utoimmune encephalomyelitis, the function of PKC-theta in Th17 differentiation remains unknown.

164 e examined whether mice with inactivation of PKC-theta are protected from fat-induced insulin resista

165 complex, which appears to be independent of PKC-theta.

166 NF-kappaB activation, whereas inhibition of PKC-theta and PKC-zeta inhibited IL-1beta-stimulated act

167 Inhibition of PKC-theta and PKC-zeta using pseudosubstrates inhibited

168 ination with other treatments, inhibition of PKC-theta may facilitate achieving long-term survival of

169 Inhibition of PKC-theta protected Treg from inactivation by TNF-alpha,

170 Consistent with this, inhibition of PKC-theta protein expression using small interfering RNA

171 Pharmacological interruption of PKC-theta increases and Dlgh1 silencing decreases the ab

172 ation, further suggesting the involvement of PKC-theta in T-cell activation.

173 Finally, arcuate-specific knockdown of PKC-theta attenuated diet-induced obesity and improved i

174 small interfering RNA-mediated knockdown of PKC-theta in Jurkat cells also resulted in apoptosis upo

175 tion induced the highest activation level of PKC-theta.

176 or the immunological synapse localization of PKC-theta and indicate V3-based 'decoys' may be therapeu

177 , neither increased membrane localization of PKC-theta nor induced insulin resistance.

178 by oral gavage increased the localization of PKC-theta to cell membranes in the hypothalamus, which w

179 ression of a constitutively active mutant of PKC-theta potently induces NF-kappaB activation and stim

180 e II (gamma) IFNs induced phosphorylation of PKC-theta in human T-cell lines and primary human T-lymp

181 Such phosphorylation of PKC-theta resulted in activation of its kinase domain, s

182 induced marked calcium flux, recruitment of PKC-theta and F-actin to the cell/bead contact site, and

183 ed redundantly to promote the recruitment of PKC-theta and subsequent polarization responses.

184 t CARMA1(L808P), restores the recruitment of PKC-theta, Bcl10, and IKKbeta into lipid rafts in CARMA1

185 To investigate the role of PKC-theta in neutrophil adhesion, we performed intravita

186 These findings suggest that the role of PKC-theta in outside-in signaling following engagement o

187 inding, it has become clear that the role of PKC-theta in T cells is complex.

188 Here, we examine the role of PKC-theta in the IS, evidence for its distinct localizat

189 R-proximal signaling and that sumoylation of PKC-theta is essential for the formation of a mature imm

190 d human T cells revealed that sumoylation of PKC-theta was essential for T cell activation.

191 ility complex (MHC) induces translocation of PKC-theta to membrane lipid rafts, which localize to the

192 at stimulation of FasL expression depends on PKC-theta-mediated activation of NF-AT pathway.

193 Surprisingly, only PKC-theta translocated to the site of cell contact.

194 lack constitutive expression of PKC-alpha or PKC-theta at detectable levels were transfected with ful

195 re transfected with full-length PKC-alpha or PKC-theta genes driven by the ecdysone promoter.

196 ad, Bid, Akt1, Akt2, PKC-mu, PKC-epsilon, or PKC-theta was achieved by transient transfection using s

197 The pharmacologic PKC-theta inhibitor (Compound 20) administered during al

198 d by punctate focusing of PKC theta, phospho-PKC theta, and phospho-ZAP70.

199 In human platelets, PKC-theta-selective antagonistic (RACK; receptor for act

200 ial molecular mediator to integrate positive PKC-theta-dependent TCR signals to induce peak RORgammat

201 se strengthening is decreased by presynaptic PKC theta.

202 h-affinity antigenic peptide did not promote PKC theta focusing in naive cells.

203 n degradation of two key signaling proteins, PKC-theta and PLC-gamma1.

204 14-3-3 tau interacted directly with purified PKC theta in vitro.

205 e results suggest that innervation regulates PKC theta and alpha isoform expression in skeletal muscl

206 F6, as well as upstream NF-kappaB regulators PKC theta, CARMA1, Bcl-10, and MALT1, which connect to t

207 cell receptor (TCR) crosslinking in the same PKC-theta-/- T cells did result in significantly decreas

208 SAP-PKC-theta interactions required R78 of SAP, a residue pr

209 Ectopic expression of V3 sequestered PKC-theta from the immunological synapse and interfered

210 Silencing PKC-theta maintains SHP-1 in the active conformation, re

211 s or wild-type cells treated with a specific PKC-theta inhibitor, but was inhibited by the PKC-theta

212 Rottlerin, a specific PKC-theta inhibitor, diminished p-CREB protein levels wh

213 KC-dependent signals and, more specifically, PKC theta-mediated functions during T-cell activation.

214 e shown that in response to TCR stimulation, PKC-theta-/- T cells undergo apoptosis due to greatly re

215 ive than wild-type 14-3-3 tau in suppressing PKC theta-dependent IL-2 promoter activity, suggesting t

216 on of SAP in T cells increased and sustained PKC-theta recruitment to the immune synapse and elevated

217 Expression of a human His6-tagged PKC-theta in Jurkat cells and purification by Ni2+ chela

218 oduction, and, conversely, membrane-targeted PKC-theta mutants rescued IL-4 expression in SAP(-/-) CD

219 We found that PKC theta-/- mice immunized with the myelin oligodendroc

220 es to the immunologic synapse indicated that PKC theta;, unlike TCR, persisted in the synapse for at

221 We conclude that PKC-theta is able to control T cell-mediated immune resp

222 We conclude that PKC-theta mediates integrin-dependent neutrophil functio

223 Collectively, our results demonstrate that PKC-theta deficiency results in an attenuated response t

224 We further demonstrate that PKC-theta displays a preference for PG vesicles over PI

225 In conclusion, our findings demonstrate that PKC-theta is a crucial component mediating fat-induced i

226 Altogether, these findings demonstrate that PKC-theta is an interferon-inducible kinase and strongly

227 In this study, we demonstrate that PKC-theta-mediated signals inhibit inducible regulatory

228 In this study, we demonstrate that PKC-theta-regulated expression of Bcl-xL is essential fo

229 Recent studies demonstrated that PKC-theta is required for the development of natural CD4

230 (+) T cells, providing genetic evidence that PKC-theta is a critical component of SLAM/SAP-mediated p

231 We found that PKC-theta but neither PKC-alpha nor PKC-epsilon particip

232 tion and immunohistochemistry, we found that PKC-theta was expressed in discrete neuronal populations

233 odels of Ag-induced arthritis and found that PKC-theta-deficient mice develop disease, but at a signi

234 We hypothesized that PKC-theta contributes to ILC2 activation and might be ne

235 These results indicate that PKC-theta functions in a unique pathway that links the T

236 Altogether, the data indicate that PKC-theta integrates the signals from TCR signaling and

237 Functional experiments indicated that PKC-theta was required for MTOC reorientation and that P

238 Here we report that PKC-theta is expressed in both human and mouse ILC2s.

239 mputerized tomographic imaging revealed that PKC-theta deficiency also protects from bone destruction

240 Here we show that PKC-theta is essential for TCR-mediated T-cell activatio

241 sistance in skeletal muscle and suggest that PKC-theta is a potential therapeutic target for the trea

242 h studies in T-cell lines had suggested that PKC-theta regulates activation of the JNK signalling pat

243 ut not PKC-theta-/- T cells, suggesting that PKC-theta plays a role in PMA-mediated inhibition of Ca2

244 P-1 and IkappaB kinase-beta, suggesting that PKC-theta stimulates Stat3 transcription via the AP-1 an

245 cardiac allograft rejection, suggesting that PKC-theta-regulated survival is required for T cell-medi

246 The PKC theta (PKCtheta) isoform is involved in TCR signal t

247 KC-theta inhibitor, but was inhibited by the PKC-theta activator PMA, or by CD28 crosslinking, which

248 Raft translocation was mediated by the PKC-theta regulatory domain and required Lck but not ZAP

249 -type mice, prevented heart rejection in the PKC-theta-/- mice.

250 articular cartilage damage were mild in the PKC-theta-deficient mice as compared with wild-type mice

251 Thus, the PKC-theta-mediated signals may function not only in the

252 , Zap70, Vav, and PI3K expression, and their PKC theta- and JNK-regulated expression/activation.

253 ich in turn recruits protein kinase C theta (PKC theta) and Bcl10 to the microdomains, causing subseq

254 Protein kinase C theta (PKC theta) is unique among PKC isozymes in its transloca

255 The protein kinase C theta (PKC theta) serine/threonine kinase has been implicated i

256 lymphocytes requires protein kinase C theta (PKC-theta) and an appropriately elevated free intracellu

257 8, to synergize with protein kinase C theta (PKC-theta) in the induction of RE/AP.

258 ) signaling molecule protein kinase C theta (PKC-theta)-mediated phosphorylation of SRC1 is important

259 Protein kinase C-theta (PKC-theta) is essential for mature T cell activation; ho

260 Protein kinase C-theta (PKC-theta) plays important roles in the activation and s

261 Protein kinase C-theta (PKC-theta) recruitment to the immunological synapse is r

262 Protein kinase C-theta (PKC-theta) translocates to the center of the immunologic

263 We now show that protein kinase C-theta (PKC-theta), a novel PKC isoform, plays a central role in

264 , the recruitment of protein kinase C-theta (PKC-theta), Bcl10, and IkappaB kinase beta (IKKbeta) int

265 se cascade involving protein kinase C-theta (PKC-theta), leading to defects in insulin signaling and

266 at in the absence of protein kinase C-theta (PKC-theta), superantigen (staphylococcal enterotoxin B)-

267 Although protein kinase C-theta (PKC-theta)-deficient mice are resistant to the induction

268 nase (PI3-K)/Akt and protein kinase C-theta (PKC-theta).

269 nant active mutant of protein kinase C theta(PKC theta) induced strong SMRT-receptor interaction in t

270 , which localize to the T cell synapse; this PKC-theta translocation is important for its function.

271 or-dependent transactivation in part through PKC theta-dependent enhancement of SMRT-receptor interac

272 Thus, PKC theta in both presynaptic and postsynaptic elements

273 Thus, PKC theta; and PKC alpha may function as positive and ne

274 Thus, PKC-theta can both positively and negatively regulate th

275 Thus, PKC-theta is identified as a major kinase within cells w

276 Similar to PKC-theta-deficient primary CD4+ T cells, small interfer

277 physiological T cell activation translocates PKC-theta to rafts, which localize to the T cell synapse

278 In this study we used PKC theta-deficient mice to investigate the potential in

279 hed with palmitic acid, are CNS mediated via PKC-theta activation, resulting in reduced insulin activ

280 o the lower levels of granule secretion when PKC-theta function is lost.

281 analysis, was significantly higher, whereas PKC-theta, -eta, and -mu were significantly lower in mus

282 first in a broad region of membrane, whereas PKC-theta arrived later in a smaller zone.

283 We determined whether PKC-theta is engaged in interferon (INF) signaling in T-

284 Preparations in which PKC theta-deficient neurons innervated normal muscle als

285 The mechanism by which PKC-theta drives innate immune cells to instruct TH2 res

286 Although the molecular interactions in which PKC-theta; engages have not been fully delineated, insig

287 In addition, CN synergizes with PKC theta; to induce RXRE-dependent activation, a cooper

288 Cbl-b associates with PKC-theta upon TCR stimulation and regulates TCR-induced

289 a but inhibited the association of CD28 with PKC-theta and filamin A and impaired the assembly of a m

290 Fyn recruitment, yet SAP's interactions with PKC-theta occurred independent of phosphotyrosine bindin

291 y reduced in human platelets pretreated with PKC-theta RACK peptide, which may contribute to the lowe

292 fts, whereas Rag1-/- mice reconstituted with PKC-theta-/- T cells failed to promote rejection.

293 Constitutively active PKC-theta, or WT PKC-theta activated by either PMA or TCR cross-linking,