ライフサイエンスコーパス: Pichia

1 synthesis of correctly processed proteins in Pichia.

2 in Xenopus oocytes and for overexpression in Pichia.

3 and could be overexpressed and purified from Pichia.

4 S. cerevisiae Cse4 and the noncomplementing Pichia angusta ortholog showed that species specificity

5 The methylotrophic Pichia angusta VKM Y-2559 and the oleaginous Cryptococcu

6 tact monomeric ATP synthase from the fungus, Pichia angusta, has been solved by electron cryo-microsc

7 crystal structure of the PROPPIN Atg18 from Pichia angusta.

8 species, and a eukaryote (the yeast species Pichia anomalia) were investigated.

9 tory peptide and placed under control of the Pichia AOX1 promoter in the vector pPIC9.

10 and was applied to two model microorganisms, Pichia augusta and Escherichia coli, cultivated on (13)C

11 rsion to prokaryote (E. coli)- or eukaryote (Pichia)-based codon usage dramatically improved the leve

12 Our results suggest that the Pichia-based EBA-F2 vaccine construct has further potent

13 ric Sup35p, containing the Sup35NM region of Pichia, can be turned into a prion in S. cerevisiae by o

14 Microsomal preparations from Pichia cells expressing AtGALT2 incorporated [(14)C]Gal

15 ased on P. falciparum, Escherichia coli, and Pichia codon usage and expressed recombinant F2 in E. co

16 The Pichia-derived antigen stimulated stronger major histoco

17 Pichia-expressed 35-kDa polypeptide, designated iridopti

18 d refolded from E. coli or produced from the Pichia expression system, is equivalent and mimics the f

19 The enzyme cDNA was subcloned into a Pichia expression vector pPIC9K to produce a recombinant

20 one each C. intermedia, C. pelliculosa, and Pichia fabianni).

21 C. inconspicua produced white colonies; the Pichia fermentans group and C. krusei did not.

22 cing the 5.8S internal transcribed spacer as Pichia fermentans, Wickerhamomyces anomalus and Candida

23 t abundant species being Trichoderma sp. and Pichia guilliermondi.

24 between another five more divergent species, Pichia guilliermondii, C. glabrata, C. zeylanoides, C. h

25 tes (Saccharomyces cerevisiae - KF551990 and Pichia gummiguttae - MCC 1273) and influence of jamun se

26 ression systems, including Escherichia coli, Pichia, insect cells, and mammalian cells.

27 anamorphic yeast Candida utilis (teleomorph: Pichia jadinii) by conventional methods.

28 da parapsilosis, Torulaspora delbrueckii and Pichia kluyveri showed greater changes in the reducing s

29 In this Opinion piece, we use Pichia (Komagataella) pastoris to illustrate the limitat

30 fluence of Saccharomyces cerevisiae (Sc) and Pichia kudriavzevii (Pk) during on-farm fermentation on

31 light subunit LAT2 in detergent-solubilized Pichia membranes, allowing functional reconstitution of

32 ing from the distantly related yeast species Pichia methanolica.

33 d and purified the p36 component of ANXII in Pichia methanolica.

34 OA is situated on the opposite inner face in Pichia mitochondria.

35 the cytosolic face of the outer membrane in Pichia mitochondria.

36 ed as a non-covalent dimer from secretion in Pichia pastoris (115 mg/l) and was a potent inhibitor of

37 hains was tested with human LAL expressed in Pichia pastoris (phLAL) and CHO cells (chLAL), respectiv

38 ng mannose terminated human LAL expressed in Pichia pastoris (phLAL), purified, and administered by t

39 Pichia pastoris (Pp) Pex8p, the only known intraperoxiso

40 been co-produced in the methylotrophic yeast Pichia pastoris (Pp).

41 s produced in both H. jecorina (HjCel3A) and Pichia pastoris (Pp-HjCel3A).

42 olded (EcCSP) or in the methylotrophic yeast Pichia pastoris (PpCSP) for structural analyses.

43 We show that the ScPex11p homologue in Pichia pastoris (PpPex11p) is phosphorylated at serine 1

44 roteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic ch

45 garicus meleagris recombinantly expressed in Pichia pastoris .

46 When Pichia pastoris adapts from methanol to glucose growth,

47 Expression of an intronless ckx1 in Pichia pastoris allowed production of large amounts of r

48 Recombinant Pichia pastoris AMA1-FVO (PpAMA1-FVO) and PpAMA1-3D7 are

49 bacter globiformis amine oxidase (AGAO), and Pichia pastoris amine oxidase (PPLO) have been examined.

50 gC2 and gD2 were produced in glycoengineered Pichia pastoris and administered intramuscularly as a bi

51 The proteins were expressed in Pichia pastoris and adsorbed on Alhydrogel.

52 1-15 domains were expressed in high yield in Pichia pastoris and baculovirus, respectively.

53 In this report, we described two systems (Pichia pastoris and baculovirus/Sf9 cells) for expressio

54 eport that MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic

55 rDer p 5 was produced in Pichia pastoris and characterized by mass spectrometry,

56 d N- and O-linked mannosylation in the yeast Pichia pastoris and compared them to their unglycosylate

57 is rat Kv1.2 which has been overexpressed in Pichia pastoris and crystallised.

58 tide, SDF-2, are conserved between the yeast Pichia pastoris and D. discoideum.

59 1 and -DBL5 recombinant proteins produced in Pichia pastoris and developed a panel of seven chondroit

60 from barley aleurone tissue was expressed in Pichia pastoris and Escherichia coli.

61 s two recombinant forms (single-chain Fab in Pichia pastoris and Fab in Escherichia coli).

62 an in vitro cell-free ER-budding assay using Pichia pastoris and followed two endogenous PMPs, Pex11p

63 e redox enzyme was recombinantly produced in Pichia pastoris and homogeneously purified, and its gluc

64 d Rlm7, the AvrLm4-7 protein was produced in Pichia pastoris and its crystal structure was determined

65 GSA12 in Pichia pastoris and its Saccharomyces cerevisiae counter

66 tic properties of VKORC1L1 when expressed in Pichia pastoris and more particularly its susceptibility

67 and the former is additionally conserved in Pichia pastoris and Paracoccus denitrificans, suggesting

68 7Y-hGALE proteins were also overexpressed in Pichia pastoris and purified for analysis.

69 d Cys-less P-glycoproteins were expressed in Pichia pastoris and purified in high yield in detergent-

70 d this aquaporin in the methylotrophic yeast Pichia pastoris and purified the hexahistidine-tagged pr

71 ant human ABCG5 and ABCG8 genes in the yeast Pichia pastoris and purified the proteins to near homoge

72 Human MATN-1 was cloned and expressed in Pichia pastoris and purified to homogeneity.

73 .1) was produced in the methylotrophic yeast Pichia pastoris and purified to near-electrophoretic hom

74 d multiple Bla g 2 mutants were expressed in Pichia pastoris and purified.

75 a-amylase expressed in amylolytic strains of Pichia pastoris and Saccharomyces cerevisiae.

76 rexpressed the human cytosolic ISCS in yeast Pichia pastoris and showed that the cytosolic form of IS

77 t was expressed in both Escherichia coli and Pichia pastoris and shown to be active.

78 x Arabidopsis GT31 members were expressed in Pichia pastoris and tested for enzyme activity.

79 oduced in glycoengineered lines of the yeast Pichia pastoris and that antibody-mediated effector func

80 WCI5 and WCI2 were expressed in Pichia pastoris and the recombinant proteins were assaye

81 hesis, the candidate genes were expressed in Pichia pastoris and their activities measured with the b

82 Recombinant thaumatin was expressed in Pichia pastoris and through a co-expression strategy wit

83 Here, we expressed recombinant hSMVT in Pichia pastoris and used affinity chromatography to puri

84 S-7 cells and the methylotropic yeast strain Pichia pastoris and was shown capable of penetrating int

85 ing peptides were expressed recombinantly in Pichia pastoris and were tested for their ability to bin

86 loned by RACE-PCR and expressed in the yeast Pichia pastoris as a secreted enzyme.

87 Our results demonstrate the utility of using Pichia pastoris as an efficient eukaryotic host to expre

88 We used the methylotrophic yeast Pichia pastoris as an expression host to produce a large

89 odium/D-glucose co-transporter 1 (hSGLT1) in Pichia pastoris as representative example of a useful st

90 DH mutants were recombinantly produced using Pichia pastoris as the host microorganism, and their IET

91 ry and expressed in the methylotrophic yeast Pichia pastoris at a secretion yield of approximately 10

92 lic forms of AMA1 that were both produced in Pichia pastoris at a sufficient economy of scale to be u

93 termined by autophagy receptors, such as the Pichia pastoris autophagy-related protein 30 (Atg30), wh

94 and pH 8.0 was purified from the engineered Pichia pastoris broth to homogeneity by anion exchange c

95 unaffected in most pex mutants of the yeast Pichia pastoris but is severely reduced in pex4 and pex2

96 lization pathway in the methylotrophic yeast Pichia pastoris by binding to Mxr1p response elements (M

97 romyces cerevisiae, the methylotrophic yeast Pichia pastoris can assimilate amino acids as the sole s

98 peroxisomal matrix, respectively, in living Pichia pastoris cells and followed by fluorescence micro

99 binant staphylokinase (SakSTAR) expressed in Pichia pastoris cells have been determined.

100 G/E56D/L72Y], was generated and expressed in Pichia pastoris cells in yields exceeding 100 mg/liter.

101 aE chain, was expressed in and purified from Pichia pastoris cells.

102 The variants were expressed in Pichia pastoris cells.

103 GT43-4, TaGT47-13, TaGT75-3, and TaGT75-4 in Pichia pastoris confirmed that these proteins form a com

104 The budding yeast Pichia pastoris contains discrete tER sites and is, ther

105 The budding yeast Pichia pastoris contains ordered Golgi stacks next to di

106 go selection.We have identified Cvt9 and its Pichia pastoris counterpart Gsa9.

107 thod is demonstrated on a flux experiment of Pichia pastoris employing two different tracers, i.e., 1

108 The recombinant enzyme expressed in Pichia pastoris established a 1.3:1 equilibrium between

109 otein obtained by heterologous expression in Pichia pastoris exhibited greater XET activity against x

110 a streamlined workflow for the generation of Pichia pastoris expression strains, reducing the timelin

111 eine-rich domain of PyP140/RON4 by using the Pichia pastoris expression system and characterized the

112 alone or domain 9 alone were expressed in a Pichia pastoris expression system and tested for their a

113 erologous expression of this cDNA clone in a Pichia pastoris expression system led to the secretion i

114 The Pichia pastoris expression system offers economy, ease o

115 To investigate this hypothesis, we used a Pichia pastoris expression system to produce large amoun

116 binant human MD-2 (rhMD-2) was produced in a Pichia pastoris expression system, and the interaction b

117 Using the Pichia pastoris expression system, we show that cleavage

118 engineering of the DNA encoding VCP into the Pichia pastoris expression system, were used to localize

119 ession by small-scale fermentation using the Pichia pastoris expression system.

120 , were produced using Escherichia coli and a Pichia pastoris expression system.

121 ase after its heterologous expression in the Pichia pastoris expression system.

122 nt maize chitinase, rChiA, was purified from Pichia pastoris extracellular medium by differential pre

123 ernal standardization by fully (13)C labeled Pichia pastoris extracts enabled absolute quantification

124 is exemplified using unclarified broth from Pichia pastoris fermentation as feedstock.

125 in product (LeMir) was produced in the yeast Pichia pastoris for generation of antibodies.

126 CS proteins expressed in Escherichia coli or Pichia pastoris for their ability to induce immunity and

127 However, FBP1 when expressed in Pichia pastoris generated H2O2 using cysteine at pH 7.2,

128 nt protein to be expressed in Yeast (such as Pichia pastoris GS115) and purified rapidly and easily w

129 Recombinant EhCP5 expressed in Pichia pastoris had kinetic properties similar to those

130 lasminogen (amino acids 93-470) expressed in Pichia pastoris had physical properties (molecular size,

131 Because yeasts such as Pichia pastoris have been shown to O-glycosylate some pr

132 Finally, we demonstrate that the Pichia pastoris homologue Gsa7p that is required for per

133 protein expressed in the methylotropic yeast Pichia pastoris indicate that it catalyzes the 4-epimeri

134 WFhb1-1 protein produced in Pichia pastoris inhibits growth of both F. graminearum a

135 Pichia pastoris is a methylotrophic yeast that has been

136 The methylotrophic yeast Pichia pastoris is a popular host for the production of

137 Pichia pastoris is a simple and powerful expression plat

138 Pichia pastoris is a yeast capable of expressing large a

139 r methanol assimilation are synthesized when Pichia pastoris is grown in methanol.

140 nd NOP-1 protein heterologously expressed in Pichia pastoris is labeled by using all-trans [3H]retina

141 The yeast Pichia pastoris is used extensively as the host cell for

142 The expression of a CSLA protein in Pichia pastoris led to the abundant production of plant

143 (EPAO), Pisum sativum amine oxidase (PSAO), Pichia pastoris lysyl oxidase (PPLO), bovine plasma amin

144 , Escherichia coli amine oxidase (ECAO), and Pichia pastoris lysyl oxidase (PPLO).

145 uman pancreatic alpha-amylase, concanavalin, Pichia pastoris lysyl oxidase, and Klebsiella pneumoniae

146 We isolated a Pichia pastoris mutant that was unable to grow on the pe

147 Growth of the yeast Pichia pastoris on methanol induces the expression of ge

148 ood yields cultivating the heterologous host Pichia pastoris on the 5L bioreactor scale (reUmChlE; 45

149 uffy binding-like (DBL) domains expressed in Pichia pastoris or var2csa plasmid DNA and sera were scr

150 the interaction between Pex19p and all known Pichia pastoris Pex proteins by the yeast two-hybrid ass

151 mport, we examined the behavior of PMPs in a Pichia pastoris pex17 mutant.

152 Pichia pastoris PEX17 was cloned by complementation of a

153 e report the cloning and characterization of Pichia pastoris PEX19 by complementation of a peroxisome

154 We show that Pichia pastoris Pex8p (PpPex8p) enters the peroxisome ma

155 The Pichia pastoris pexophagy receptor Atg30 is recruited to

156 Furthermore, we also demonstrated that Pichia pastoris produces XynCDBFV with higher catalytic

157 in of rat neural agrin (AgG3z8) expressed in Pichia pastoris promoted AChR clustering on surface of C

158 We show how a Pichia pastoris protein, PpAtg30, mediates peroxisome se

159 e effects of bile acids on ATP hydrolysis in Pichia pastoris purified ABCG5/G8 and found that they st

160 se mutation in a cysteine protease, G79E, in Pichia pastoris resulted in an unstable precursor protei

161 ologous expression of this gene in the yeast Pichia pastoris resulted in the production of a beta-1,4

162 P2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression o

163 inant Tum-5 produced in Escherichia coli and Pichia Pastoris specifically inhibited proliferation and

164 ructosidase (BfrA) secreted by a recombinant Pichia pastoris strain was optimally immobilised on Glyo

165 oped a platform using genetically engineered Pichia pastoris strains designed to secrete multiple pro

166 In the Pichia pastoris system, the protease domain was expresse

167 mutant) were overproduced recombinantly in a Pichia pastoris system, they displayed the dual inhibito

168 in and expressed in the methylotrophic yeast Pichia pastoris to obtain a post-translationally modifie

169 ed and expressed in the methylotrophic yeast Pichia pastoris to probe for the proposed phosphatidylch

170 Here, we established an expression system in Pichia pastoris to recombinantly produce and purify Cx43

171 on of the glycosylation pathway in the yeast Pichia pastoris to secrete a human glycoprotein with uni

172 xpanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with full

173 Here we successfully generated a Pichia pastoris transformant expressing and secreting ap

174 into the genome of the methylotrophic yeast Pichia pastoris under the control of an AOX promoter and

175 s expressed as a secreted soluble protein in Pichia pastoris under the regulation of alcohol oxidase

176 s nivalis agglutinin; GNA) were expressed in Pichia pastoris using native signal peptides, or the Sac

177 s were observed against both rh-Endo and the Pichia pastoris vector, but no allergic reactions were o

178 The istk gene was expressed in Pichia pastoris vectors.

179 xpression system in the methylotrophic yeast Pichia pastoris was developed.

180 e recombinant protein expressed in the yeast Pichia pastoris was found to have activity against the i

181 The secretory pathway of Pichia pastoris was genetically re-engineered to perform

182 herapy, an Fv-p53 fusion protein produced in Pichia pastoris was tested on CT26.CL25 colon cancer cel

183 In-cell NMR in the yeast Pichia pastoris was used to study the influence of metab

184 ress this, we expressed human CFH mutants in Pichia pastoris We found that recombinant I62-CFH (prote

185 ACC synthase is now expressed in Pichia pastoris with an improved yield of 10 mg/L.

186 ed in overexpressing PfCRT to high levels in Pichia pastoris yeast by synthesizing a codon-optimized

187 uced in single Escherichia coli bacteria and Pichia pastoris yeast cell in the current study.

188 sma membrane of Saccharomyces cerevisiae and Pichia pastoris yeast.

189 hancer, we expressed recombinant alpha2AP in Pichia pastoris yeast.

190 Chy1 was expressed in Pichia pastoris yielding an enzyme with a chymotrypsin s

191 s of 27,653 Daltons, was expressed in yeast (Pichia pastoris) and purified by anion exchange column c

192 In Komagataella phaffii (formerly called Pichia pastoris) specifically, the indirect traffic of P

193 e full-length enzyme (expressed in the yeast Pichia pastoris) were quantified.

194 study of expression of the RK gene in yeast (Pichia pastoris), COS-1 cells and in an HEK293 stable ce

195 lus nidulans) and in a methylotrophic yeast (Pichia pastoris), the latter expression system producing

196 Mutant enzymes were expressed in Pichia pastoris, a methylotrophic yeast strain, and thei

197 ; EC1.6.6.1) were cytosolically expressed in Pichia pastoris, a methylotrophic yeast, using spinach (

198 CPTI and CPTII were separately expressed in Pichia pastoris, a yeast with no endogenous CPT activity

199 es were expressed in Escherichia coli and in Pichia pastoris, and analyzed for monoclonal antibody an

200 n domain 11, expressed these mutant forms in Pichia pastoris, and determined binding kinetics with hu

201 ic human cysteine protease, was expressed in Pichia pastoris, and its physicokinetic properties were

202 Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, and mammalian cell lines.

203 eu, or Trp, the mutant proteins expressed in Pichia pastoris, and purified to homogeneity.

204 he polypeptide was recombinantly produced in Pichia pastoris, and the three-dimensional structure was

205 genesis-defective (pex) mutants of the yeast Pichia pastoris, AOX fails to assemble into active octam

206 and a deglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocat

207 When expressed in Pichia pastoris, AxlA had activity comparable to the nat

208 of a form of P. falciparum AMA1, produced in Pichia pastoris, by vaccinating Aotus vociferans monkeys

209 Purified recombinant PHACS, expressed in Pichia pastoris, contains bound pyridoxal-5'-phosphate (

210 All three Na-GSTs, when expressed in Pichia pastoris, exhibited low lipid peroxidase and glut

211 we report that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost nor

212 coprotein (Pgp; mouse MDR3) was expressed in Pichia pastoris, grown in fermentor culture, and purifie

213 The methylotrophic yeast, Pichia pastoris, has been genetically engineered to prod

214 man bile salt-stimulated lipase expressed in Pichia pastoris, hen ovalbumin, bovine fetuin, bovine th

215 lypeptide ABC transporter TAPL, expressed in Pichia pastoris, into lipid vesicles (liposomes) and per

216 A. fumigatus Cu,Zn SOD has been expressed in Pichia pastoris, is enzymatically active, and has bioche

217 a water transporting aquaporin of the yeast Pichia pastoris, is suggested to be gated by chemo-mecha

218 (AOXI) promoter of the methylotrophic yeast, Pichia pastoris, is used widely for the production of re

219 ressed this gene heterologously in the yeast Pichia pastoris, obtaining a relatively high yield of 2.

220 alpha2beta1, alpha2beta2, and alpha2beta3 in Pichia pastoris, purified the complexes, and compared th

221 d recombinant PI8 in the methylotropic yeast Pichia pastoris, purified the inhibitor to homogeneity,

222 ssed different variants of MDR3 in the yeast Pichia pastoris, purified the proteins via tandem affini

223 n was expressed recombinantly in E. coli and Pichia pastoris, resulting in unglycosylated and mannosy

224 common with a glycosylated form expressed in Pichia pastoris, the [(15)N,(1)H]-correlation spectra of

225 In Pichia pastoris, the orientation of a 138-kb invertible

226 In Pichia pastoris, the peroxisomal targeting signal 2 (PTS

227 AT2) heterodimers overexpressed in the yeast Pichia pastoris, together with docking analysis and cros

228 almitoyltransferase I (L-CPT I) expressed in Pichia pastoris, two contiguous discrete sequences withi

229 er liter levels in the methylotrophic yeast, Pichia pastoris, using the methanol oxidase promoter.

230 A binding domain [BoNT/A(Hc)], expressed in Pichia pastoris, was developed as a vaccine candidate fo

231 f human recombinant cathepsin F, produced in Pichia pastoris, was processed to its active mature form

232 ion assays of BdCSLF6 expressed in the yeast Pichia pastoris, we also demonstrate that the catalytic

233 e isolation of peroxisomal import mutants in Pichia pastoris, we have isolated a mutant (pex7) that i

234 e this idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Sa

235 m freezing damage, we transformed the yeast, Pichia pastoris, with an inducible DEA1 construct.

236 enicity of the natural allergen, whereas the Pichia pastoris-derived glycosylation does not.

237 CD4 chimeric proteins in mammalian cells and Pichia pastoris.

238 g dominant-negative mutant forms of Sar1p in Pichia pastoris.

239 se by PCR, and the mutants were expressed in Pichia pastoris.

240 In this work, nicaTf was expressed in Pichia pastoris.

241 Human IgG1-Fc was first overproduced in Pichia pastoris.

242 amples of human growth hormone secreted from Pichia pastoris.

243 ation of human ABC transporters in the yeast Pichia pastoris.

244 strate membrane insertion in both E.coli and Pichia pastoris.

245 ylase has now been successfully expressed in Pichia pastoris.

246 pexophagy of methanol-induced peroxisomes in Pichia pastoris.

247 mbinant protein expressed in and secreted by Pichia pastoris.

248 secretion pathway of the methylotropic yeast Pichia pastoris.

249 es domain 5 alone (Dom5His) was expressed in Pichia pastoris.

250 mh2 was heterologously produced in the yeast Pichia pastoris.

251 sed as a recombinant proprotein in the yeast Pichia pastoris.

252 d at discrete tER sites in the budding yeast Pichia pastoris.

253 ase (nXGase) were produced heterologously in Pichia pastoris.

254 and expressed recombinant F2 in E. coli and Pichia pastoris.

255 ne and cytoplasmic domains, was expressed in Pichia pastoris.

256 sed recombinant human clusterin in the yeast Pichia pastoris.

257 We explored this issue using the yeast Pichia pastoris.

258 ified recombinant Epo (R103A) from the yeast Pichia pastoris.

259 albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris.

260 1, PH2, and PH4 proteins after expression in Pichia pastoris.

261 a soluble, secretory protein using the yeast Pichia pastoris.

262 ADE1, and URA3-from the methylotrophic yeast Pichia pastoris.

263 nds retinal after heterologous expression in Pichia pastoris.

264 ltration of recombinant proteins produced by Pichia pastoris.

265 inant form of AChE C was highly expressed by Pichia pastoris.

266 ve (pex) mutants of the methylotrophic yeast Pichia pastoris.

267 acterization of the FLD1 gene from the yeast Pichia pastoris.

268 deletion mutants were expressed in the yeast Pichia pastoris.

269 n both chinese hamster ovary (CHO) cells and Pichia pastoris.

270 uired for peroxisome biogenesis in the yeast Pichia pastoris.

271 and the recombinant protein was expressed in Pichia pastoris.

272 ed 50% of the total supernatant protein from Pichia pastoris.

273 C-terminal half of VCP has been expressed in Pichia pastoris.

274 hate dehydrogenase gene (GAP) from the yeast Pichia pastoris.

275 in and secreted by the methylotrophic yeast Pichia pastoris.

276 Z2 formed single rings in cells of the yeast Pichia pastoris.

277 ytic domain of CA IX in methylotrophic yeast Pichia pastoris.

278 myces boulardii, Saccharomyces paradoxus, or Pichia pastoris.

279 We tested these ideas using the yeast Pichia pastoris.

280 roduced in 293T cells, Escherichia coli, and Pichia pastoris.

281 llus subtilis xylanase A (XynA) expressed in Pichia pastoris.

282 duced by recombinant expression in the yeast Pichia pastoris.

283 r protein expression in Escherichia coli and Pichia pastoris.

284 The abf3 gene was thus expressed in Pichia pastoris.

285 iogenesis genes in the methylotrophic yeast, Pichia pastoris.

286 ence selected for heterologous expression in Pichia pastoris.

287 pecies were synthesized in the yeast species Pichia pastoris: K195M, K199M, F211V, W214L, R218M, R222

288 ed in the methylotrophic yeast Komagataella (Pichia) pastoris.

289 n three yeast species, Candida parapsilosis, Pichia philodendra and Candida salmanticensis, is charac

290 ations, the antibodies generated against the Pichia-produced protein prevented the binding of recombi

291 the microsomal fractions of Arabidopsis and Pichia, respectively.

292 coccus, Filobasidium, Kloeckera, Malassezia, Pichia, Sporidiobolus, Rhodotorula, Zygosaccharomyces, a

293 s, such as Brettanomyces, Hanseniaspora, and Pichia spp.

294 Pichia stipitis is a well-studied, native xylose-ferment

295 tive sequence contains Ile at position 116 ( Pichia stipitis OYE 2.6) revealed that this enzyme's ste

296 We studied a mutant strain of Pichia stipitis, a yeast capable of converting xylose to

297 erevisiae by overproduction of the identical Pichia Sup35NM.

298 n the heterologous system, overproduction of Pichia Sup35p or Sup35NM induced formation of the prion

299 ng MLG not only in tobacco cells but also in Pichia, which generally does not produce MLG.

300 We demonstrate that Pichia yeast produce 'normally functioning' mammalian Kv