ライフサイエンスコーパス: Pol delta

1 ric mutator variant of DNA polymerase delta (Pol delta).

2 B and identified it as DNA polymerase delta (Pol delta).

3 DL but does not affect primer recognition by Pol delta.

4 ry subunit of the replicative DNA polymerase Pol delta.

5 nal mismatches to enable primer extension by Pol delta.

6 s PCNA at the DNA nick generated by FEN1 and Pol delta.

7 s with accessory subunits of replicative DNA Pol delta.

8 n cellular DNA replication than depletion of Pol delta.

9 properties in comparison with Pol alpha and Pol delta.

10 es the rate and fidelity of DNA synthesis by Pol delta.

11 ogous mutations at position Leu-606 in human pol delta.

12 ady state kinetic studies of the fidelity of pol delta.

13 on by the active site than that of wild type pol delta.

14 n are distinct from the errors made by human Pol delta.

15 nd processivity similar to that of wild type pol delta.

16 than for those same mismatches when made by Pol delta.

17 d recombinagenic responses to the failure of Pol delta.

18 prevented by the 3.-exonuclease activity of Pol delta.

19 t implicates PP1 in the dephosphorylation of Pol delta.

20 ved motif A in the polymerase active site of Pol delta.

21 spectrum characteristic of mistakes made by Pol delta.

22 fidelity of DNA synthesis in vitro by yeast pol delta.

23 condary UV irradiation, and DNA synthesis by Pol delta.

24 of the rate and fidelity of DNA synthesis by pol delta.

25 shared with the replicative DNA polymerase, pol delta.

26 MMEJ substrates following DSB induction than Pol delta.

27 Mammalian DNA polymerase delta (pol delta), a key enzyme of chromosomal DNA replication,

28 periments with catalytically inactive mutant Pol delta(AA).

29 Depletion of the Pol delta accessory subunit POLD2 destabilizes the compl

30 ase PCNA is an allosteric modulator of other Pol delta activities.

31 His-tagged mature PDIP38 inhibited pol delta activity in vitro and interacted with human pa

32 zyme, suggesting that CMG may bind and alter Pol delta activity or position it on the lagging strand.

33 icate that p12 contributes to PCNA-dependent pol delta activity, i.e. the p12-PCNA interaction is fun

34 ain the regulation of the oxidation state on Pol delta activity, possibly useful during cellular oxid

35 at both p12 and p68 are required for optimal pol delta activity.

36 s in Saccharomyces cerevisiae using a mutant Pol delta allele (pol3-L612M) whose error rate is higher

37 ase errors are similar to those generated by pol delta alone, indicating that pol delta itself is by

38 Pol delta also extends mismatched primers 3 times more s

39 L612M pol delta also has normal 3' exonuclease activity, yet i

40 As described in yeast and unlike Pol theta, Pol delta also promotes homology-directed repair.

41 ide or short oligonucleotide displacement by pol delta and cleavage by FEN1.

42 Furthermore, mutations in Pol delta and DNA polymerase 4 (Pol lambda), the DNA pol

43 and LigI remain on the DNA, indicating that Pol delta and FEN1 dissociate during 5' end processing a

44 CNA binding, as well as purified recombinant pol delta and its subassemblies, were analyzed.

45 Here we describe a novel interaction between Pol delta and LigI that is critical for Okazaki fragment

46 Pol delta and Mgs1 may collaborate either in strand anne

47 the hypothesis that the interaction between pol delta and PCNA is a divalent one that involves p12 a

48 lication by the replicative DNA polymerases, Pol delta and Pol .

49 Pol delta and Pol alpha, which conduct lagging strand re

50 Pol delta and pol epsilon are essential, but their roles

51 ubstitution of the high-fidelity replicative pol delta and pol epsilon with the TLS pol theta, pol et

52 he multi-subunit DNA polymerases: Pol alpha, Pol delta and Pol epsilon.

53 chain elongation by proofreading-proficient pol delta and pol epsilon.

54 A onto the DL and the extension of the DL by Pol delta and Pol eta.

55 locking lesions occur by the exchange of the Pol delta and Pol zeta catalytic subunits on a preassemb

56 conserved and similar C-terminal domains of Pol delta and Pol zeta contain a [4Fe-4S] cluster coordi

57 ating cell nuclear antigen) binds tightly to Pol delta and recruits it to the lagging strand.

58 though high-fidelity DNA polymerases such as pol delta and the bacteriophage T4 DNA polymerase replic

59 Unexpectedly, replicative pol delta and the error-prone translesion synthesis pol

60 Both Pol delta and WRN encode 3' --> 5' DNA exonuclease activ

61 Yeast Pol delta and yeast Pol eta both bypass 8-oxoG and misin

62 OFP due to excessive strand displacement by pol delta and/or by an as yet unidentified helicase.

63 DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon) are

64 H2, Ku, DNA-PKcs, MCM proteins, PCNA and DNA Pol delta) and in protein metabolic processes, including

65 primer and extended by DNA polymerase delta (pol delta) and the replication clamp proliferating cell

66 DNA Polymerase delta (Pol delta) and the Werner syndrome protein, WRN, are inv

67 at takes place in both DNA polymerase delta (Pol delta)- and DNA polymerase (Pol )-dependent MMR reac

68 yeast Pol eta is 10-fold more efficient than Pol delta, and following bypass Pol eta switches to less

69 y three B-family DNA polymerases: Pol alpha, Pol delta, and Pol .

70 erases alpha, delta, and epsilon (Pol alpha, Pol delta, and Pol epsilon), are responsible for eukaryo

71 three replicative DNA polymerases Pol alpha, Pol delta, and Pol epsilon; and canonical maturation of

72 ivity of proliferating cell nuclear antigen, Pol-delta, and Pol-epsilon, and it contributes to the ph

73 Intercrosses of Pol epsilon-, Pol delta-, and mismatch repair-mutant mice show that Po

74 Error rates in vitro for L612M pol delta are elevated for both base substitutions and s

75 ken together, the changes that p12 exerts on Pol delta are ones that can modulate its fidelity of DNA

76 ts, deficient in the exonuclease activity of Pol delta are partially deficient in the repair of these

77 lon as the leading strand DNA polymerase and Pol delta as the lagging strand polymerase during undist

78 ent issue of Molecular Cell, they now assign Pol delta as the lagging strand polymerase.

79 its of the replicative DNA polymerase delta (Pol delta) as promoters of Alt-NHEJ that results in more

80 This leads to stalling of pol delta at 8-oxo-G after incorporation of correct C.

81 promised more than lagging-strand polymerase Pol delta at low dNTP concentrations in vitro.

82 ced telomere synthesis requires the RFC-PCNA-Pol delta axis, but is independent of other canonical re

83 ilon binds CMG with a Kd value of 12 nM, but Pol delta binding CMG is undetectable.

84 Unexpectedly, Pol delta binds only one subunit of the PCNA trimer.

85 Pol delta binds to proliferating cell nuclear antigen (P

86 isingly, the main lagging-strand polymerase, Pol delta, binds the leading strand upon uncoupling and

87 We find that Pol delta bound to DNA is indeed redox-active at physiol

88 In eukaryotes, DNA polymerase delta (Pol delta) bound to the proliferating cell nuclear antig

89 imulates the strand displacement activity of Pol delta but it also allows efficient replication throu

90 d upon inactivation of the 3' exonuclease of pol delta but not that of pol epsilon.

91 m the fork progression, or by recruitment of Pol delta, but not Pol epsilon, to post-replicative proc

92 e catalytic rate of Saccharomyces cerevisiae Pol delta by >10-fold.

93 This transformation of Pol delta by modification of its quaternary structure is

94 ults demonstrate that the [4Fe4S] cluster in Pol delta can act as a redox switch for activity, and we

95 hat Pol alpha-primase and the lagging-strand Pol delta can be re-used within the replisome to support

96 irmed that the 3'-5'-exonuclease activity of Pol delta can efficiently remove these DNA lesions.

97 Under conditions where Pol delta carries out strand displacement synthesis, the

98 The Pol delta catalytic rates in the absence and presence of

99 wer CMG, and in the absence of a stabilizing Pol delta-CMG interaction, the collision release process

100 nucleotide incorporation by a preformed DNA-Pol delta complex in the absence of PCNA was 40 s-1.

101 In contrast to rad51Delta mutants, the Pol delta complex mutants are proficient for repair of a

102 due to strand invasion failure, whereas the Pol delta complex mutants are proficient for strand inva

103 The half crossovers formed in Pol delta complex mutants show evidence of limited homol

104 for components of the DNA polymerase delta (Pol delta) complex.

105 ar results were obtained for yeast and human Pol delta complexes.

106 In addition to its catalytic subunit (p125), pol delta comprises three regulatory subunits (p50, p68,

107 In contrast, although Pol delta contacts the nascent lagging strands of active

108 ivities and reaction products of recombinant pol delta containing a p12 mutant defective in PCNA bind

109 In the more common short flap pathway, Pol delta coordinates with the flap endonuclease FEN1 to

110 On the lagging strand, Pol delta corrects errors made by Pol alpha during Okaza

111 It was found that pol delta could catalyze high-fidelity synthesis past NM

112 We now observe that similarly to WRN, Pol delta degrades alternate DNA structures including bu

113 alysis of DNA synthesis progression by human Pol delta demonstrated significant synthesis perturbatio

114 The association of Pol delta depended on RAD1, which encodes the flap endon

115 e extent of strand displacement synthesis by Pol delta determines whether maturation proceeds by the

116 e proposed pathway for flap removal involves pol delta displacement of long flaps, coating of those f

117 howed that in the presence of PCNA and FEN1, pol delta displacement synthesis favors formation and cl

118 Primer extension by DNA polymerase delta (pol delta) displaces the downstream fragment into an RNA

119 Our finding that Pol delta dissociates at specific CFS sequences is signi

120 This approach identifies DNA pol alpha, DNA pol delta, DNA pol varepsilon, MCM2-7, Cdc45, GINS, and

121 on cryo-EM structure of the human processive Pol delta-DNA-PCNA complex in the absence and presence o

122 is at least 10-fold more accurate than yeast Pol delta during 8-oxoG bypass.

123 3'-ends of Okazaki fragments synthesized by Pol delta during DNA replication.

124 Both LigI and FEN1 associate with PCNA-Pol delta during gap-filling synthesis, suggesting that

125 trand synthesis switches from Pol epsilon to Pol delta during replication termination.

126 l zeta as well as diminished proofreading by Pol delta during replication.

127 2) also regulates the subunit composition of Pol delta during the cell cycle.

128 ex' lesions, demonstrating the importance of Pol delta during the repair of DNA lesions in close prox

129 ed by a "collision release" process in which pol delta ejects from PCNA upon extending a DNA template

130 GMP or A-dCMP for T to C transitions), L612M pol delta error rates are substantially higher for one m

131 the basic biochemical properties that allow Pol delta-exo(-) to carry out strand displacement synthe

132 Pol delta exonuclease removes 3'-terminal mismatched nuc

133 Pol delta extends the upstream Okazaki fragment and disp

134 Polymerase delta (Pol delta) extends the upstream Okazaki fragment and dis

135 human specialized Pols from three families: Pol delta (family B), Pol eta and Pol iota (family Y), a

136 nthesis is carried out by a complex of PCNA, Pol delta, FEN1 and LigI.

137 y efficiently coordinating the activities of Pol delta, FEN1, and Lig1.

138 The p12 subunit alters Pol delta fidelity by modulating the proofreading 3' to

139 n of the activities of DNA polymerase delta (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I

140 racts with the enzymes DNA polymerase delta (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I

141 Moreover, WRN and Pol delta form a complex with enhanced ability to hydrol

142 eta binds to the Pol31 and Pol32 subunits of Pol delta, forming a four-subunit Pol zeta(4) complex (R

143 We provide evidence that Pol delta forms higher ordered complexes upon binding to

144 f this conclusion, we show that depletion of Pol delta from cells reduces BIR, whereas depletion of P

145 d DNA and promotes the rapid dissociation of pol delta from PCNA on stalling at a DNA lesion.

146 Dpb2 does not affect Pol delta function with CMG, and thus we propose that th

147 ovel in vivo mechanism for the modulation of Pol delta function.

148 polymerase in eukaryotes, polymerase delta (pol delta), functions with the proliferating cell nuclea

149 Compared with wild type pol delta, L612M pol delta has normal processivity and slightly higher po

150 intrinsic to the pol3/pol31 subunits of the pol delta heterotrimer.

151 cerevisae where the extreme stability of the pol delta holoenzyme ensures that every nucleobase withi

152 However, the stability of the human pol delta holoenzyme is unknown.

153 nalyze the processivity and stability of the pol delta holoenzyme.

154 characteristic of the human DNA polymerase (Pol delta) holoenzyme (150 bp for one binding event), co

155 function of the human DNA polymerase delta (Pol delta) holoenzyme by comparing the kinetics of DNA s

156 inct from that of L868M/F Pol alpha or L612M Pol delta, implying that each polymerase has different a

157 eta, or eta, all of which are able to assist pol delta in 8-oxo-G bypass by translesion synthesis (TL

158 tions generated over 33 generations by L612M Pol delta in a mismatch repair defective strain.

159 d DNA, but the activity was not shut down by Pol delta in accord with rapid association/dissociation

160 eavage products on flaps strand-displaced by pol delta in an oligonucleotide system reconstituted wit

161 lso impacts our understanding of the role of Pol delta in both DNA replication and DNA repair.

162 the 3.2- angstrom cryo-EM structure of S.c. Pol delta in complex with primed DNA, an incoming ddTTP,

163 nuclease activity and its cooperativity with Pol delta in preserving genome stability, which is compr

164 l zeta extended the DL more efficiently than Pol delta in the absence of PCNA, but little or no stimu

165 NA binding affinities and stoichiometries by Pol delta in the absence of PCNA, using electrophoretic

166 UV treatment converts Pol delta in vivo to the three-subunit form lacking p12.

167 s of recombinant human DNA polymerase delta (Pol delta) in which one or two of the three conserved As

168 We found that pol delta incorporates nucleotides with an error frequen

169 ination positively regulates TLS to overcome Pol delta inhibition.

170 DNA polymerase delta (Pol delta) initiates BIR; however, it is not known wheth

171 elta (lagging strand polymerase) and Mgs1 (a pol delta interacting protein) lead to a defect in fusio

172 DNA Pol delta-interacting protein 2 (PolDIP2) has been found

173 We propose a model in which pol delta interacts with PCNA via at least two of its su

174 Yeast pol delta is a heterotrimer comprised of three subunits:

175 Saccharomyces cerevisiae Pol delta is a three-subunit enzyme (Pol3-Pol31-Pol32).

176 Pol delta is also crucial for efficient recoupling of le

177 Pol delta is anchored to one of the three PCNA monomers

178 In this report, Saccharomyces cerevisiae pol delta is examined on model substrates to gain insigh

179 Surprisingly, we find pol delta is highly processive with PCNA, over at least

180 In the absence of p12, Pol delta is more likely to proofread DNA synthesis beca

181 In vitro, the generation of short flaps by Pol delta is not affected by the presence of Pol32; howe

182 to function on the lagging strand, even when Pol delta is not present.

183 Although pol delta is one of the major eukaryotic replicative pol

184 strand bias strongly supports the idea that Pol delta is primarily a lagging strand polymerase durin

185 Previous studies show that Pol delta is slow and distributive with CMG on the leadi

186 DNA polymerase delta (pol delta) is a high fidelity eukaryotic enzyme that par

187 DNA polymerase delta (Pol delta) is a key enzyme in mammalian chromosomal repl

188 Eukaryotic DNA polymerase delta (pol delta) is a member of the B family of polymerases an

189 veral types of repair, DNA polymerase delta (pol delta) is assisted by replication protein A (RPA), t

190 Mammalian DNA polymerase delta (Pol delta) is believed to replicate a large portion of t

191 rtantly, we found that DNA polymerase delta (Pol delta) is critical for MMEJ, independent of microhom

192 DNA polymerase delta (Pol delta) is one of the major replicative DNA polymeras

193 DNA polymerase delta (pol delta) is one of the two main replicative polymerase

194 In eukaryotes, DNA polymerase delta (pol delta) is responsible for replicating the lagging st

195 DNA polymerase delta (Pol delta) is responsible for the elongation and maturat

196 DNA polymerase delta (Pol delta) is thought to catalyze DNA synthesis to fill

197 Although Pol32 is an integral component of Pol delta, it is also required for translesion synthesis

198 enerated by pol delta alone, indicating that pol delta itself is by far the prime determinant of fide

199 he overall pol delta-PCNA complex as well as pol delta itself.

200 nstrated that heterozygous expression of the pol delta L604G variant in mice results in normal life s

201 The pol delta L606G and L606K holoenzymes retain catalytic a

202 pol delta L606G is highly error prone, incorporating sin

203 high frequency during DNA synthesis, whereas pol delta L606K is extremely accurate, with a higher fid

204 However, pol delta L606K is impaired in the bypass of DNA adducts

205 amino acid in yeast Pol alpha (L868M/F) and Pol delta (L612M), these data indicate that the active s

206 Compared with wild type pol delta, L612M pol delta has normal processivity and s

207 g mutants of DNA polymerases; defects in DNA pol delta (lagging strand polymerase) and Mgs1 (a pol de

208 Pol delta, like bacterial replicases, undergoes collisio

209 urprisingly, the results indicate that human pol delta maintains a loose association with PCNA while

210 We suggest that reduction in the level of Pol delta may be less deleterious because of its collisi

211 gesting that PDIP38 might play a role in the pol delta-mediated viral DNA replication.

212 post-replication act as partial barriers to Pol-delta-mediated displacement of Pol-alpha-synthesized

213 icate that the exonuclease activity of human Pol delta modulates its intrinsic bypass efficiency on t

214 tivity can be explained by the dependency of pol delta mutants on Chk1 activation for survival.

215 showed substantially less stimulation of the Pol delta nucleotide incorporation rate, identifying the

216 The high processivity of pol delta observed in this report contrasts with its rol

217 by Pol epsilon on the leading strand and by Pol delta on the lagging strand.

218 ision release process is triggered, ejecting Pol delta on the leading strand.

219 s in humans as it limits the processivity of pol delta on undamaged DNA and promotes the rapid dissoc

220 bulk replication by proofreading-proficient Pol delta or Pol epsilon.

221 that deletion of the POL32 (third subunit of Pol delta) or PIF1 helicase genes can suppress lethality

222 We also show that CK2 phosphorylates the Pol delta p125, p68, and p12 subunits and that these pho

223 s with 7-deaza-G residues, did not alleviate Pol delta pause sites within the G residues.

224 Here we show that replicative pol delta pauses in front of 8-oxo-G and displays diffic

225 Using DNA trap experiments, we show that Pol delta pauses within CFS sequences are sites of enzym

226 upon completing replication, and we propose Pol delta-PCNA collides with the slower CMG, and in the

227 could play a role in stabilizing the overall pol delta-PCNA complex as well as pol delta itself.

228 However, Saccharomyces cerevisiae Pol delta-PCNA is a rapid and processive enzyme, suggest

229 We have shown earlier that Pol delta-PCNA is suppressed on the leading strand with

230 Conversely, Pol delta-PCNA is the only enzyme capable of extending O

231 nd eluted column fraction II (CFII) included pol delta, pol eta, and RFC.

232 ppressed by further deletion of a subunit of pol delta, POL32.

233 However, in the presence of pol beta, pol delta preferentially facilitates hairpin retention r

234 Pol delta processivity was enhanced by full length Werne

235 plates with the G4 ligand BRACO-19 inhibited Pol delta progression into the G-rich repeats.

236 ining Okazaki fragment length by restricting Pol delta progression.

237 ogether, these data support a model in which Pol delta promotes Alt-NHEJ in human cells at DSBs, incl

238 mutator phenotype), and inactivation of both Pol delta proofreading (pol3-01) and MMR is lethal due t

239 MMEJ recombinants showed evidence that Pol delta proofreading function is active during MMEJ-me

240 ly different from those triggered by loss of Pol delta proofreading.

241 hich causes defects in DNA polymerase delta (Pol delta) proofreading (pol3-01) and nucleotide selecti

242 ng the hypothesis that the 3' exonuclease of pol delta proofreads errors generated by pol alpha durin

243 nd PCNA are included in reactions containing pol delta, rates for single base errors are similar to t

244 on was observed for Pol epsilon but not with Pol delta, RB69 gp43 or Pol eta.

245 However, Pif1 working with pol delta readily unwound a full-length Okazaki fragment

246 Moreover, Pol delta recruitment was diminished in cells lacking Po

247 Pol delta replicates and matures Okazaki fragments on th

248 d by hydrolytic end sequencing, we show that Pol delta replicates both the invading and the complemen

249 main of Pol3, suggesting that all aspects of Pol delta replication are important to human health and

250 ubstitutions identified "hotspot" motifs for Pol delta replication errors.

251 on origin, in a manner implying that >90% of Pol delta replication is performed using the lagging str

252 t that, unlike its prokaryotic counterparts, pol delta requires replication accessory factors like pr

253 s of many of the amino acid substitutions in Pol delta resemble those of previously identified antimu

254 ates are primarily copied by Pol epsilon and Pol delta, respectively.

255 The corresponding mutation in purified human Pol delta results in a similar 30-fold increase in mutat

256 to-Gln substitution in the fingers domain of Pol delta results in an interaction with the N-terminal

257 ssays following electrochemical oxidation of Pol delta reveal a significant slowing of DNA synthesis

258 ested a role for yeast DNA polymerase delta (Pol delta), RFC and PCNA in LLR repair synthesis.

259 In the presence of purified FEN1, Pol delta, RFC and PCNA, repair occurred on heteroduplex

260 work sheds light on the structural basis of Pol delta's activity in replicating the human genome.

261 rinsic 3'-exonuclease activity, implies that Pol delta should have high base substitution fidelity.

262 lating the binding of Pol zeta with Pol32 in Pol delta stalled at a lesion site.

263 sis, are observed when either Pol epsilon or Pol delta stalls at leading-strand damage, and do not re

264 tionally, a fold-back in the template slowed pol delta synthesis, so that the fragment could be remov

265 Somewhat surprisingly, we found that pol delta synthesizes DNA at a slow rate with a k(pol) o

266 Pol delta synthesizes the lagging strand during replicat

267 rystal structure of Saccharomyces cerevisiae Pol delta template reveals four potential contacts for A

268 NA-DNA hybrids are more readily displaced by Pol delta than DNA hybrids, thereby favoring degradation

269 ions that encode amino acid substitutions in Pol delta that suppress this lethal phenotype.

270 ve for FEN1(RAD27) and the 3.-exonuclease of Pol delta) that produce long flaps and of dna2Delta muta

271 ated by two DNA polymerases, Pol epsilon and Pol delta, that function on the leading and lagging stra

272 ol epsilon being the main leading strand and Pol delta the lagging strand DNA polymerase.

273 e primer for the continuous DNA synthesis by pol delta, thereby leading to hairpin retention and repe

274 kappa was significantly more efficient than Pol delta through both types of repetitive elements.

275 ablish predominance of DNA polymerase delta (Pol delta) through its POLD3 subunit.

276 on exposure to DNA-damaging agents, converts Pol delta to a form that has an increased capacity for p

277 of a single Rap1 molecule; Pif1 also allows Pol delta to carry out DNA synthesis across an array of

278 nt of Pol delta to the DL but stimulation of Pol delta to displace a DNA strand during DL extension.

279 suggesting an active hand-off mechanism from Pol delta to FEN1(RAD27).

280 g its fidelity, facilitating the switch from Pol delta to Pol lambda during 8-oxo-G TLS.

281 t C:8-oxo-G can be overcome by a switch from pol delta to pols lambda, beta, or eta, all of which are

282 Here we investigate the contribution of Pol delta to replication of the leading and lagging stra

283 ial role of PCNA in HR is not recruitment of Pol delta to the DL but stimulation of Pol delta to disp

284 erating cell nuclear antigen (PCNA) recruits Pol delta to the DNA and serves as a processivity factor

285 nstituted in a purified system with FEN1 and Pol delta, together with PCNA and its loader RFC.

286 The released pol delta transfers to a new primed site, provided the n

287 expressing the error-prone but not wild-type Pol delta variant, which is not observed following treat

288 Four-subunit human pol delta variants that harbor or lack 3' --> 5'-exonucl

289 Pol delta was significantly inhibited in regions contain

290 Consistently, Pol epsilon, but not Pol delta, was found in nuclear matrix fraction througho

291 , the functional roles of the p12 subunit of pol delta were studied.

292 al errors generated by DNA polymerase delta (Pol delta), which takes over for Pol alpha to complete l

293 mical properties of Saccharomyces cerevisiae pol delta with a methionine replacing conserved leucine

294 Interaction of Pol delta with PCNA eliminates flap-mediated inhibition

295 xo1 substitutes for FEN1 and Pol epsilon for Pol delta with reasonable efficiency.

296 placement synthesis by DNA polymerase delta (Pol delta) with 5.-flap cutting by FEN1(RAD27) endonucle

297 icases in human cells, DNA polymerase delta (Pol delta), with an error-prone variant allows increased

298 ded PCNA greatly stimulates DNA synthesis by Pol delta within the DL but does not affect primer recog

299 we provide evidence that PolDIP2 stimulates Pol delta without affecting its fidelity, facilitating t

300 present evidence that WRN can proofread for Pol delta; WRN excises 3'-terminal mismatches to enable