ライフサイエンスコーパス: R. rubrum

1 R. rubrum puf interposon mutants do not form intracytopl

2 that encode a CO-oxidation system, allowing R. rubrum to use CO as a sole energy source.

3 f the redox dependence of CO(2) reduction by R. rubrum CODH show that (1) CODH is unable to catalyze

4 the gas-sensing regulation mechanism used by R. rubrum CooA and its homologs in other organisms, we c

5 Instead, under aerobic growth conditions, R. rubrum RLP employs another intermediate of the MSP, 5

6 recently described hybrid enzyme containing R. rubrum alpha and beta subunits and the CF1 gamma subu

7 In contrast, R. rubrum CooA, which is exquisitely specific for CO, fo

8 This system enables R. rubrum to grow in the dark on CO as the sole energy s

9 nt potential of the [Fe(4)S(4)] cluster from R. rubrum Fe protein.

10 Previously, CooJ from R. rubrum (RrCooJ) has been described as a nickel chaper

11 I) and Escherichia coli (ecI), and dIII from R. rubrum (rrIII) and E. coli (ecIII) were overexpressed

12 +/2+) cluster of nitrogenase Fe protein from R. rubrum or Azotobacter vinelandii.

13 -fold more efficient than DraT purified from R. rubrum, but with a similar K(m) value for NAD(+).

14 an be functionally promiscuous, RuBisCO from R. rubrum is not promiscuous for either of the known RLP

15 We then examined the ability of RuBisCO from R. rubrum to catalyze both of the RLP-catalyzed reaction

16 thionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway

17 , whose activity is regulated by NH(4)(+) in R. rubrum.

18 tional regulation of nitrogenase activity in R. rubrum under these conditions.

19 required for activation of NifA activity in R. rubrum, GlnK and GlnJ do not appear to be involved in

20 nzymes are regulated in vivo as described in R. rubrum.

21 When examined for physiological effects in R. rubrum, these GlnB* variants activated NifA in the pr

22 sting that a paralog of P(II) might exist in R. rubrum and regulate the modification of GS.

23 When expressed in R. rubrum either as single-copy integrants or on multipl

24 The effect of the lack of bluB function in R. rubrum was reflected by the impaired ability of a Del

25 a, glnD deletion mutations are not lethal in R. rubrum.

26 resence of functional, yet separate, MSPs in R. rubrum under both aerobic (chemoheterotrophic) and an

27 P-ribosylation, of nitrogenase Fe protein in R. rubrum.

28 ave implications for DRAT-DRAG regulation in R. rubrum.

29 d intermediate for the biosynthesis of RQ in R. rubrum.

30 causative basis for the poor growth seen in R. rubrum mutants lacking P(II) and presumably in mutant

31 ity appears to be comparable to that seen in R. rubrum.

32 r uptake and accumulation of (63)Ni(2+) into R. rubrum and to observe the effect of mutations in the

33 with several deletions, were introduced into R. rubrum by homologous recombination.

34 DPH (H2NADPH) bound very tightly to isolated R. rubrum dIII, but the rate constant for dissociation w

35 ne encoding the RLP abolishes the ability of R. rubrum to utilize 5'-methylthioadenosine as a sole su

36 The identification of the acidocalcisomes of R. rubrum was carried out by using transmission electron

37 Cultures of R. rubrum were grown in the presence of synthetic analog

38 s, we studied the heterologous expression of R. rubrum draTG in Klebsiella pneumoniae glnB and glnK m

39 PHA accumulation in enhancing the fitness of R. rubrum was indicated by the relationship between PHA

40 A mutant of R. rubrum in which the rate of oxidation of Fe protein w

41 these results suggest that the H(+)-PPase of R. rubrum has two distinct roles depending on its locati

42 ly important residues in the CooA protein of R. rubrum.

43 e results indicate that the C(red1) state of R. rubrum CODH (E(m) = -110 mV; g(zyx)() = 2.03, 1.88, 1

44 mplete genome sequence of a mutant strain of R. rubrum (F11), which cannot grow anaerobically and doe

45 ucture is also compared to the structures of R. rubrum domain I with NAD bound (PDB code 1F8G) and wi

46 ckout strains and complementation studies of R. rubrum.

47 of wild-type CooA from its native organism, R. rubrum, to greater than 95% purity.

48 DRAG had <2% activity with oxidized R. rubrum Fe protein relative to activity with reduced F

49 ibiting low (<20%) activity with 87% reduced R. rubrum Fe protein relative to activity with fully oxi

50 ependent transcription of the CooA-regulated R. rubrum promoter PcooF in vitro, which indicates that

51 ast few dissimilar residues from the related R. rubrum homolog increased the enzyme's kcat for carbox

52 However, unlike B. subtilis RLP, R. rubrum RLP does not catalyze the enolization of 2,3-d

53 genes encoded in a contiguous region of the R. rubrum genome.

54 quired to sustain CO-dependent growth of the R. rubrum mutants demonstrated different phenotypes: whe

55 We show that the R. rubrum porins Por39 and Por41 form a helical ribbon-l

56 The CODH family, for which the R. rubrum enzyme is the prototype, catalyzes the biologi

57 I+ecIII mixtures as well as in the wild-type R. rubrum and possibly in the E. coli enzyme.

58 e generated a deletion mutant from wild-type R. rubrum by the targeted replacement of rquA with a gen

59 Analysis of wild-type R. rubrum grown on nickel-depleted medium indicates a re

60 Surprisingly, and unlike R. rubrum CooA, C. hydrogenoformans CooA binds NO to for

61 ibe several in vivo feeding experiments with R. rubrum used for the identification of RQ biosynthetic