ライフサイエンスコーパス: RACE

1 RACE (Rapid Amplification of cDNA Ends) identified a 1.5

2 RACE (rapid amplification of cDNA ends) PCR is useful fo

3 RACE analysis extended the sequence and identified this

4 RACE and RT-PCR identified a splice variant of FKBP8 lac

5 RACE describes all alveoli that visibly change volume wi

6 RACE experiments then mapped the 3' terminus of the upst

7 RACE is 55-330 times faster and 2-5 times more accurate

8 RACE is easy to use, as it requires adjustment of only t

9 RACE mapping revealed that lpiA/acvB were co-transcribed

10 RACE processed these terabyte-sized datasets on a single

11 RACE was used to identify one major and two minor transc

12 RACE-RepSeq VJ full-length sequencing additionally revea

13 3' RACE provided evidence for variability in the 3' UTR due

14 Real-time PCR and 5' RACE and 3' RACE experiments revealed that this locus encodes two tr

15 for the QQS mRNA was identified by 5' and 3' RACE experiments.

16 were obtained by assembling their 5' and 3' RACE polymerase chain reaction products.

17 hain reaction (RT-PCR) followed by 5' and 3' RACE showed that Sh-NOS is a protein of 1,517 amino acid

18 lable immunoglobulin sequences and 5' and 3' RACE to clone and sequence heavy and light chain immunog

19 some 2 were designed, and, through 5' and 3' RACE, clones from 506 genes were sequenced and cDNA sequ

20 nce, reverse transcription-PCR and 5' and 3' RACE.

21 bout 6 kb through conventional RT-PCR and 3' RACE.

22 gene structures were delineated by 5' and 3' RACE.

23 and polyadenylation sites were defined by 3' RACE.

24 In 3' RACE, the poly(A) tail functions as a non-specific tag a

25 o nt 7299 or 7307, respectively, by using 3' RACE.

26 Examination of mGL50 cDNA transcripts by 3'RACE revealed an alternatively spliced form, mGL50-B, th

27 Using 3'RACE, we confirmed expression of two distinct forms of t

28 In addition, using 3'RACE, we identified three previously unknown mGlu1 recep

29 5'-and 3'-RACE experiments have subsequently identified a 900-bp c

30 5'- and 3'-RACE experiments identified a total of 11 R. reniformis

31 T47D breast carcinoma cells by RT-PCR and 3'-RACE PCR and identified a novel extended form of QSOX1 t

32 e use of these conditions yielded 5'- and 3'-RACE products that were approximately 80% GC over 213 an

33 108-158 nt) through genomic analysis and 3'-RACE technique, which was confirmed by RNA blot analysis

34 As an initial step, we performed 5'- and 3'-RACE with SULT1C1 cDNA.

35 ed and verified using RPE/choroid 5'- and 3'-RACE.

36 uence of MIH, in conjunction with 5'- and 3'-RACE.

37 RNA-sequencing data, followed by 5'- and 3'-RACE.

38 ctive polyadenylation sites identified by 3'-RACE are conserved in human, mouse, and chicken SCN8A.

39 from the one for UT-A2 and identified by 3'-RACE new transcripts of UT-A1, UT-A2, and UT-A3, charact

40 5'-methylguanosine-capped RNA followed by 3'-RACE sequencing.

41 amplification of complementary DNA ends (3'-RACE) polymerase chain reaction, we identified a chimeri

42 We used 5'-or 3'-RACE, GenomeWalking, reverse transcriptase polymerase ch

43 Subsequent 3'-RACE, 5'-RACE and high fidelity PCR were then used to pr

44 d RNA-Seq procedures, as well as a 1200 bp 5 RACE product coupled with PACBio sequencing that can ide

45 5' RACE analysis revealed the presence of a previously unde

46 5' RACE analysis revealed the presence of the transcription

47 5' RACE analysis was performed to determine the functional

48 5' RACE analysis, promoter-reporter assays, and semiquantit

49 5' RACE carried out with CWR22Rv1 and HepG2 cells detected

50 5' RACE indicates a transcription start site for HYDIN2 out

51 5' RACE studies localized the initiation sites to a <100 bp

52 l clones in mediating tumor regression, a 5' RACE technique was used to determine the distribution of

53 Real-time PCR and 5' RACE and 3' RACE experiments revealed that this locus en

54 validation using RNA cleavage assays, and 5' RACE identified the prooncogenic basic helix-loop-helix

55 that gene was then cloned by both 3' and 5' RACE.

56 ' end of the K12 transcript was mapped by 5' RACE (rapid amplification of cDNA ends) and S1 nuclease

57 ranscription start site was identified by 5' RACE (rapid amplification of complementary DNA ends).

58 criptional start sites were determined by 5' RACE analysis.

59 y and late transcripts were identified by 5' RACE and primer walking techniques.

60 ha were mapped within the third intron by 5' RACE and S1 nuclease protection.

61 transcript initiation sites identified by 5' RACE is located 159 nucleotides upstream of the putative

62 onic promoter of miR-9-5 was validated by 5' RACE PCR.

63 ptional initiation site was identified by 5' RACE techniques using total RNA from NIH 3T3 cells.

64 This was followed by sequencing of cloned 5' RACE products and of products re-amplified from excised

65 We employed 5' RACE to isolate the 5' end of the fusion transcript and

66 ed by rapid amplification of 5' cDNA end (5' RACE), RT-PCR analysis and genome sequence analyses.

67 r to rapid amplification of 5' cDNA ends (5' RACE) for HIV-1 RNA and quantitative reverse transcripta

68 and 5' rapid amplification of cDNA ends (5' RACE) to be located 25 nt upstream of the ATG in exon 1.

69 The classical scheme for 5' RACE described here is simple, suffices in many instance

70 led with library screening and a modified 5' RACE-PCR strategy, resulted in the identification and ch

71 ys, and independently with inverse PCR of 5' RACE clones, common mRNA initiation sites were identifie

72 By means of 5' RACE cloning methodology, a novel putative full-length h

73 The 3000 BP upstream of 5' RACE product were subcloned into a pGL3 luciferase repor

74 Interestingly, analysis of 5' RACE products for antisense transcripts and the GenBank

75 The approach was based on 5' RACE with [32P]-labelled primers and separation of produ

76 genomic structure of BMPR1A, we performed 5' RACE from lymphoblastoid cell lines and normal colon tis

77 We performed 5' RACE on mRNA isolated from murine chondroprogenitor cell

78 le in mediating tumor regression and that 5' RACE analysis may provide an important tool for the anal

79 The 5' RACE analysis of the human testis mRNA revealed the exis

80 f the sequence variation defined from the 5' RACE products.

81 Results from the 5' RACE-PCR methods were consistent among themselves but di

82 with yeast or human DBR1 enzyme prior to 5' RACE and qRT-PCR.

83 rt sites of 69 rpoH-dependent genes using 5' RACE (5' rapid amplification of cDNA ends), which allowe

84 Using 5' RACE (rapid amplification of cDNA ends) analysis, multip

85 rame (ORF) of 3.5 kb was elucidated using 5' RACE and genomic sequence.

86 e 5' end of the murine Rad51l2 cDNA using 5' RACE technique as well as by sequencing the genomic regi

87 MS1 proximal promoter, initially by using 5' RACE to map transcription start sites.

88 Using 5' RACE we have now identified a novel BCL-X non-coding exo

89 Using 5' RACE, two transcription initiation sites were identified

90 Using 5' RACE, we have isolated four additional exons of the mu o

91 Using 5' RACE, we identified three promoters, designated A, B and

92 Using 5' RACE, we identified three transcription start sites and

93 rt sites in human MTHFR were mapped using 5' RACE.

94 5' -RACE and primer extension identified a long 5' -untransl

95 5'RACE followed by functional promoter analysis in multipl

96 cer-testis Ag NY-ESO-1 were cloned using a 5'RACE method from RNA isolated from a CTL generated by in

97 Bioinformatic and 5'RACE (rapid amplification of cDNA ends) analyses of the

98 RT-PCR and 5'RACE experiments confirm the presence of four additional

99 The results of RT-PCR and 5'RACE suggest that only PRB is inactivated, although PRA

100 results obtained using global RNA-Seq and 5'RACE.

101 We applied 5'RACE and a deep sequencing-based approach to investigate

102 H1 and vnfH transcriptional start sites by 5'RACE (5' rapid amplification of cDNA ends) revealed that

103 DNA sequence analysis of cDNA generated by 5'RACE from CSF1R coding sequences identified a novel fusi

104 ' rapid amplification of cDNA ends method (5'RACE), and immunohistochemical staining.

105 Rapid amplification of 5'-cDNA ends-PCR (5'RACE-PCR) revealed at least three novel forms of the unt

106 o alternative promoter was observed by RLM-5'RACE PCR and reverse transcriptase PCR analyses during e

107 amplification of 5' cDNA ends by PCR (RLM-5'RACE PCR) analysis of C. cellulovorans RNA identified a

108 Additionally, sequence analysis of the 5'RACE-PCR products revealed multiple transcriptional star

109 Using 5'RACE, we demonstrate that Giardia promoters are a source

110 in vitro DNA-binding assays combined with 5'RACE, that BrlR binds to its own promoter, likely via a

111 5'-RACE (rapid amplification of cDNA ends) data indicate th

112 5'-RACE analysis defined transcript start sites for sbi, ef

113 5'-RACE analysis of mouse embryo cDNA targeting five candid

114 5'-RACE identified p53 mRNA transcribed from a novel start

115 5'-RACE mapped the transcription start site for one of the

116 d several alternative splice sites with a 5'-RACE approach.

117 rmed by RNA-Sequencing, and extended by a 5'-RACE assay and Northern blotting, showing that meiotic c

118 A 5'-RACE study determined the transcriptional start site of

119 This prompted additional 5'-RACE experiments that established a second, TATA box-con

120 Database analysis and 5'-RACE (rapid amplification of cDNA ends) identified a 419

121 RT-PCR and 5'-RACE analysis revealed that cdr and bb0729 are co-transc

122 ive RT-PCR and used for Northern blot and 5'-RACE analysis.

123 scripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcripts with

124 RT-PCR and 5'-RACE mapping showed that the 5' UTR has a length of 1.94

125 ere cloned from a skin library by 3'- and 5'-RACE reactions.

126 cDNA library screening and 5'-RACE revealed that the major transcript encoded an open-

127 ning strategy (RT-PCR followed by 3'- and 5'-RACE) to clone from Y-organs of the blue crab (Callinect

128 Using RT-PCR and 5'-RACE, we observed that following transcriptional activat

129 enes by using a combination of RT-PCR and 5'-RACE.

130 cleavage of a subset of these targets by 5'-RACE analysis.

131 anscriptional start of human CRLR cDNA by 5'-RACE and cloned the proximal 5'-flanking region of the g

132 by comparing the repertoires generated by 5'-RACE PCR and multiplex PCR.

133 tes of all three genes were determined by 5'-RACE revealing large leader sequences for each transcrip

134 d associated promoter (P1), was mapped by 5'-RACE to a region 19 kb upstream of the ZFP106 translatio

135 nt upstream of the ATG initiation site by 5'-RACE.

136 Rapid amplification of 5'-cDNA ends (5'-RACE) analysis demonstrated exclusive use of the CBS -1b

137 5'-rapid amplification of cDNA ends (5'-RACE) and computational analyses were used to identify c

138 Rapid amplification of 5'-cDNA ends (5'-RACE) and reverse transcription-PCR assays identified sh

139 and 5'-rapid amplification of cDNA ends (5'-RACE) revealed five major ADAR1 transcriptional start si

140 Rapid amplification of cDNA ends (5'-RACE) was used to isolate the CDT6 promoter from an adap

141 cs analysis, rapid analysis of cDNA ends (5'-RACE), and reverse transcription coupled with qPCR using

142 ing 5' random amplification of cDNA ends (5'-RACE), and the binding sites for purified HlyU were disc

143 ing rapid amplification of the cDNA ends (5'-RACE), we identified one transcription start site (TSS).

144 entary deoxyribonucleic acid (cDNA) ends (5'-RACE).

145 ngest 5'-untranslated region derived from 5'-RACE and apparently generated by the distal promoter has

146 Here we report the isolation by genomic 5'-RACE PCR and in vitro analysis of the mouse PIASgamma pr

147 oth endothelial cells and liver; however, 5'-RACE analysis (rapid amplification of cDNA ends) identif

148 We obtained evidence by modified 5'-RACE for a MYB mRNA cleavage product guided by miR828 in

149 RNA, whose cleavage was shown by modified 5'-RACE.

150 question, we conducted deep sequencing of 5'-RACE products of the Igh repertoire in pro-B cells, ampl

151 he neuronal channel SCN8A, we carried out 5'-RACE (rapid amplification of cDNA ends) with RNA from hu

152 We also performed 5'-RACE experiments on 62 novel regions, and 76% of the reg

153 Subsequent 3'-RACE, 5'-RACE and high fidelity PCR were then used to produce a f

154 eight were further confirmed with the RLM 5'-RACE experiments.

155 uently full-length cDNA was cloned by the 5'-RACE (rapid amplification of cDNA ends) technique and se

156 The 5'-RACE analysis of RARbeta2 mRNA in these cells demonstrat

157 , both the RNase protection assay and the 5'-RACE assay detected endogenous pim-1 transcripts with sh

158 d" antibody repertoires by sequencing the 5'-RACE PCR products of B-cell transcripts from IAVI donor

159 By using 5'-RACE, alignment of EST sequences, and a luciferase-repor

160 o be mediated by an RNAi mechanism, using 5'-RACE.

161 s in vitro and murine enterocytes in vivo.5'-RACE identified two novel exons, 1A and 1B, which encode

162 Using Primer walking, 5'-RACE PCR and in silico analyses, we characterized nine J

163 EST libraries for OSC fragments to use in a RACE PCR-based approach and cloned three full-length OSC

164 Additional RACE and Northern analysis revealed that at least five d

165 end-polymerase chain reaction amplification (RACE-PCR).

166 Reverse transcription-PCR amplification and RACE were used to acquire the former menthone:(-)-(3R)-m

167 Reverse transcription-PCR amplification and RACE were used to acquire the remaining 5'-sequence from

168 which 6 were confirmed by northern blot and RACE.

169 This work demonstrates Raman-DIP and RACE are effective culture-independent approach for rapi

170 was obtained and verified through RT-PCR and RACE (rapid amplification of cDNA ends).

171 The gene was amplified by RT-PCR and RACE of the poly(A)+RNA isolated from the mouse melanoma

172 s of this novel gene using interexon PCR and RACE technologies.

173 ction of oligonucleotide primers for PCR and RACE-derived cDNAs from which the complete sequence of f

174 novel sequences by using degenerate PCR and RACE.

175 dii Using a combination of transcriptome and RACE sequencing, we identified 33 opsin transcripts expr

176 We apply RACE-Seq to 398 human lncRNA genes in seven tissues, lea

177 orporating other transcriptomic data such as RACE, CAGE, and EST into its model to further increase i

178 yosuroides hydrolase (Amgdsh1) was cloned by RACE-PCR and expressed in the yeast Pichia pastoris as a

179 sequence of the DCAL-1 gene was confirmed by RACE-PCR; however, based on sequence alignment with geno

180 All corresponding cDNAs were confirmed by RACE.

181 using bioinformatic predictions, followed by RACE, RT-PCR, and sequencing.

182 We then cloned the novel gene by RACE-PCR, and analysis of the deduced cDNA sequence foun

183 ons of the mouse OR genes were identified by RACE-PCR.

184 ouse ovarian adapter-ligated cDNA library by RACE-PCR, and a unique 2043-bp open reading frame was de

185 s or siRNA and analyzed cleavage products by RACE.

186 ate Control Versus Electrical Cardioversion (RACE) trials that anticoagulation should not be disconti

187 Current RACE methods often produce a high background of nonspeci

188 e extremely GC-rich sequences, using current RACE methods.

189 nfarction in Carolina Emergency Departments (RACE) project, transported via emergency medical service

190 Here we describe RACE-Seq, an experimental workflow designed to address t

191 Many different RACE methods have been developed to meet various require

192 Raman activated cell ejection (RACE) was applied to isolate single AMR bacteria for the

193 5' Random amplification of cDNA ends (RACE) analyses identified two distinct promoters, p1 and

194 5' rapid amplification of cDNA ends (RACE) analyses of RNAs prepared from G50DblKo and wild-t

195 3'-Rapid amplification of cDNA ends (RACE) analyses of the terminated RNA products allowed pr

196 5' rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify the di

197 erns using rapid amplification of cDNA ends (RACE) and full-length cDNA sequencing, revealed four ind

198 such as 5'-Rapid Amplification of cDNA Ends (RACE) and inverse polymerase chain reaction (PCR).

199 fied by 3' rapid amplification of cDNA ends (RACE) and Northern blot analyses in several EBV-positive

200 ations, 3' rapid amplification of cDNA ends (RACE) and polymerase chain reactions (PCR) were performe

201 luding the rapid amplification of cDNA ends (RACE) and tiling array technologies that was used to fur

202 ted by the rapid amplification of cDNA ends (RACE) approach.

203 5' and 3' rapid amplification of cDNA ends (RACE) experiments and findings of novel splicing events

204 ction, and rapid amplification of cDNA ends (RACE) experiments indicate the presence of multiple tran

205 m adapting rapid amplification of cDNA ends (RACE) for large-scale structural transcript annotation.

206 ies and by rapid amplification of cDNA ends (RACE) from liver, spleen and lung RNA.

207 sis and 3' rapid amplification of cDNA ends (RACE) in placenta confirmed the existence of distal intr

208 Rapid amplification of cDNA ends (RACE) is widely used to determine the 5'- and 3'-termina

209 mRNA using rapid amplification of cDNA ends (RACE) PCR as long as part of the mRNA sequence is known;

210 sults from rapid amplification of cDNA ends (RACE) PCR suggest that there are multiple transcriptiona

211 t using 5' rapid amplification of cDNA ends (RACE) PCR.

212 The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clo

213 5'- and 3'-rapid amplification of cDNA ends (RACE) product and assembling the sequences, we generated

214 5'- and 3'-Rapid Amplification of cDNA Ends (RACE) revealed IGH/CHST11 as well as CHST11/IGH fusion R

215 have used rapid amplification of cDNA ends (RACE) to identify multiple transcription initiation and

216 Rapid amplification of cDNA ends (RACE) was performed on RNA isolated from human RPE cells

217 Rapid amplification of cDNA ends (RACE) was used to obtain potentially full-length transcr

218 method of rapid amplification of cDNA ends (RACE) was used to obtain their cDNA sequences from 11 cD

219 A by 5'/3' rapid amplification of cDNA ends (RACE), 5' radiolabeling, and exonuclease digestion, whic

220 ned using random amplification of cDNA ends (RACE), and promoter regions were compared with orthologu

221 encing, 3' rapid amplification of cDNA ends (RACE), and tiled microarray analyses.

222 (RT-PCR), rapid amplification of cDNA ends (RACE), and transient expression of minigene constructs.

223 ed with 5' rapid amplification of cDNA ends (RACE), in vitro transcription assays, real-time quantita

224 etected by rapid amplification of cDNA ends (RACE), primer extension, and ribonuclease protection ass

225 Using rapid amplification of cDNA ends (RACE), reverse-transcription polymerase chain reaction (

226 , using 5' rapid amplification of cDNA ends (RACE), two transcriptional start sites (TSSs) and two pu

227 Using 3' rapid amplification of cDNA ends (RACE), we mapped the 3' end of the N and NSs mRNAs, show

228 y using 5' rapid amplification of cDNA ends (RACE), we mapped two HPV18 transcription start sites (TS

229 Rapid amplification of cDNA ends (RACE)-PCR extension of expressed sequence-tag sequences

230 solated by rapid amplification of cDNA ends (RACE).

231 loning and rapid amplification of cDNA ends (RACE).

232 scripts by rapid amplification of cDNA ends (RACE).

233 T-PCR) and rapid amplification of cDNA ends (RACE).

234 y by using rapid amplification of cDNA ends (RACE).

235 ermined by rapid amplification of cDNA ends (RACE).

236 ssed by 5' Rapid Amplification of cDNA Ends (RACE).

237 pid amplification of complementary DNA ends (RACE), chemical inhibition experiments, and genetic disr

238 pid amplification of complementary DNA ends (RACE), two transcriptional start sites were identified.

239 pid amplification of complementary DNA ends (RACE)-polymerase chain reaction (PCR) and three multiple

240 repetitive alveolar collapse and expansion" (RACE).

241 the Real-time Accurate Cell-shape Extractor (RACE), a high-throughput image analysis framework for au

242 We describe here an improved RACE procedure using circular cDNA templates and demonst

243 Thus, the term "collapse" in RACE refers to a visibly obvious collapse of the alveolu

244 by the development of alveolar instability (RACE) and the increase in alveolar size at peak inspirat

245 We furthermore integrated RACE with our framework for automated cell lineaging and

246 We have used RNA ligase mediated RACE and in silico analyses to locate two sets of transc

247 tokine termed IL-17D was cloned using nested RACE PCR.

248 kine, termed IL-17F, was cloned using nested RACE PCR.

249 'New RACE' (rapid amplification of cDNA ends) PCR is a method

250 Thus, we developed a new RACE method that can be used for this purpose.

251 Although 'new RACE' can also be used to amplify 3' ends, only the prot

252 In 'new RACE', an anchor is ligated to the 5' end of the mRNA be

253 of effector action, the requirement for NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1) is shared.

254 Arabidopsis (Arabidopsis thaliana) NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1), a plasma membr

255 We obtained RACE data for approximately two-thirds of the examined t

256 Sequence analysis of RACE products showed that the truncation is due to a sin

257 We demonstrate the generality of RACE by extracting cell-shape information from entire Dr

258 By means of RACE PCR, three full-length cDNAs not reported previousl

259 l workflow designed to address this based on RACE (rapid amplification of cDNA ends) and long-read RN

260 ing 5' rapid amplification of cDNA ends-PCR (RACE-PCR), and strong sigma(54) and sigma(70) consensus

261 by PCR-rapid amplification of cDNA ends (PCR-RACE).

262 diated Rapid Amplification of cDNA Ends (PPM-RACE).

263 ementary DNA ends-polymerase chain reaction (RACE-PCR) on patient RNA, rabaptin-5 was identified as a

264 Gene reporter, RACE, and chemical inhibitor studies demonstrated that t

265 from Hydra vulgaris using 3'- and 5'- (RLM) RACE approaches.

266 Experiments using 5'RLM-RACE demonstrated that the genes EpSPL1, 2, 3, 4, 7, 9,

267 transcriptome profiling combined with 5'-RLM-RACE analysis in transgenic plants confirmed that amiRNA

268 In the current work, we have utilized 5'-RLM-RACE to examine the influence of CGG repeat number on th

269 diated rapid amplification of cDNA ends (RLM-RACE) and RT-PCR to identify four transcription start si

270 diated rapid amplification of cDNA ends (RLM-RACE) between -61 and -32 bp from the translation initia

271 diated rapid amplification of cDNA ends (RLM-RACE) PCR analysis indicated that the single transcripti

272 diated rapid amplification of cDNA ends (RLM-RACE) reads, and 50,000 cap-trapped expressed sequence t

273 their 5' and 3' UTR were amplified using RLM-RACE.

274 e corrected proactively with our large-scale RACE platform.

275 on of cDNA ends-based repertoire sequencing [RACE-RepSeq]), we detected a lambda LC monoclonal expans

276 otting, reverse transcription-PCR, and SMART-RACE analyses suggest that the dlt transcript begins 250

277 med systematic series of pseudogene-specific RACE analyses.

278 lve altered alveolar mechanics, specifically RACE and alveolar overdistension.

279 sted gene-specific primer and the 3' or 5' T-RACE primer results in specific amplification of cDNA en

280 Overall, we show that RACE-Seq is an effective tool to annotate an organism's

281 The RACE amplification technology was used on a novel CYP3A-

282 The RACE II (Rate Control Efficacy in Permanent Atrial Fibri

283 The sequences of the RACE sorted cells indicate that they were potential huma

284 In addition, high-throughput RACE successfully extended the 5' and/or 3' ends of >60%

285 sing this targeted approach, high-throughput RACE, we revealed numerous transcripts including many un

286 Thus, RACE-RepSeq appears as a sensitive, rapid, and specific

287 associated with the 5'-ends of at least two RACE products.

288 Using RACE PCR, reverse transcription-PCR (RT-PCR) and RNA-seq

289 Using RACE procedures, we cloned and sequenced the complete 4.

290 Using RACE, we automatically reconstructed cellular-resolution

291 sis of the 5'- and 3'-ends of the mRNA using RACE analysis determined that the ADH4 mRNA in C57BL/6 m

292 th cDNAs of each product were obtained using RACE-PCR.

293 nfirmed its 3' end nucleotide sequence using RACE-PCR.

294 of supporting evidence and tested them using RACE-PCR.

295 se results show that direct validation using RACE-PCR can be an important component of genome-wide va

296 simple, suffices in many instances in which RACE is needed and can be performed in 1-3 days.

297 With RACE, molecular cloning, and long read sequencing, we fo

298 thology, whereas lung tissue from areas with RACE mechanics demonstrated alveolar collapse, atelectas

299 RT-PCR sequencing in combination with RACE on ab initio gene predictions could be used to defi

300 ing strand-specific RT-PCR complemented with RACE and FISH.