ライフサイエンスコーパス: RAMP2

1 with a receptor activity-modifying protein (RAMP2).

2 the receptor associated modifying protein 2 (RAMP2).

3 ecifically with RAMP3, but not with RAMP1 or RAMP2.

4 hanisms that regulate the trafficking of CLR*RAMP2.

5 F-1 when CRLR was co-expressed with RAMP1 or RAMP2.

6 regulation of AM-induced degradation of CLR*RAMP2.

7 t 2.9 angstrom resolution in the presence of RAMP2.

8 CGRP and AM prefer the RAMP1 and RAMP2/3 complexes, respectively, whereas AM2/IMD is thou

9 Our studies suggest that RAMP2 acts as a negative allosteric modulator of GCGR by

10 downstream signaling, we describe here that RAMP2 acts as a specific allosteric modulator of PTH1R,

11 We demonstrate that RAMP2 alters both ligand selectivity and G protein prefe

12 ing protein (RAMP)-1, RAMP1 (AMY(1)R), human RAMP2 (AMY(2)R), or human RAMP3 (AMY(3)R).

13 To understand the role of RAMP2 and -3 on the activation and conformation of the C

14 enes such as Expi (Wdnm1), Cyp1b1, Gelsolin, Ramp2 and class I MHC genes.

15 Degradation of CLR*RAMP2 and CLRDelta9KR*RAMP2 was not dependent on the dur

16 Therefore, to determine whether RAMP2 and RAMP3 have distinct functions in vivo, we gene

17 Nevertheless, our studies reveal that RAMP2 and RAMP3 have distinct physiological functions th

18 However, mRNA encoding RAMP2 and RAMP3 was also detected in the gastrointestina

19 IMD and CRLR, RAMP1, RAMP2 and RAMP3 were expressed in all cell types.When ce

20 eceptor activity-modifying proteins 2 and 3 (RAMP2 and RAMP3), respectively.

21 vo, including the ligand-receptor couple ADM-RAMP2 and SVEP1.

22 otein receptor activity-modifying protein-2 (RAMP2), and the role of individual G protein alpha-subun

23 VAT gene expression of ADM receptors Crlr, Ramp2, and Ramp3 was increased (p < 0.05) in P-HFHS dams

24 RAMP2 apparently does not interact with GCGR in an order

25 In addition, mice with reduced expression of RAMP2 (but not RAMP3) display remarkable subfertility.

26 The interaction of MRGPRX4 with RAMP2, but not RAMP1 or 3, causes attenuation of basal a

27 Pre-treatment (4 days) of HAECs with CRLR or RAMP2, but not RAMP1 or RAMP3, siRNAs abolished protecti

28 LR-RAMP3 complex, but not CRLR-RAMP1 or CRLR-RAMP2 complex, altered receptor trafficking to a recycli

29 imer to predict the structure of the MRGPRX4-RAMP2 complex.

30 d the receptor activity-modifying protein 2 (RAMP2) comprise a receptor for adrenomedullin (AM).

31 ellular domain (ECD) and tethered RAMP1- and RAMP2-CTR ECD fusion proteins and antagonist peptides.

32 ut not to HRS knockdown, whereas CLRDelta9KR*RAMP2 degradation was unaffected.

33 We show that RAMP2 directly interacts with the glucagon receptor (GCG

34 Accordingly, mutant RAMP1 W84A- and RAMP2 E101A-CTR ECD retained AC413/rAmy binding.

35 HDX-MS experiments demonstrate that RAMP2 enhances local flexibility in select locations in

36 CGRP analog-bound CLR:RAMP1 and AM-bound CLR:RAMP2 extracellular domain heterodimers at 2.5 and 1.8 A

37 CLR/RAMP1 forms a CGRP receptor, CLR/RAMP2 forms an adrenomedullin-1 (AM(1)) receptor, and CL

38 ivity for CGRP/AM in part by RAMP1 Trp-84 or RAMP2 Glu-101 contacting the distinct CGRP/AM C-terminal

39 receptor agonists and highlights a role for RAMP2 in regulating its pharmacology.

40 Additionally, RAMP2 increases both PTH- and PTHrP-triggered beta-arres

41 t the glucagon receptor that is abolished by RAMP2 interaction.

42 Strikingly, we found that, although RAMP2 is required for survival, mice that lack RAMP3 app

43 the C terminus of RAMP3, but not in RAMP1 or RAMP2, leads to protein-protein interactions that determ

44 known that AM induces internalization of CLR*RAMP2, little is known about the molecular mechanisms th

45 Moreover, RAMP2 modulates PTH1R downstream signaling in an agonist

46 AM-, calcrl-, or RAMP2-null mice died mid-gestation after development of

47 e, adrenomedullin receptors are comprised of RAMP2 or RAMP3 (AM1R and AM2R, respectively) and calcito

48 ligand, adrenomedullin (AM), is comprised of RAMP2 or RAMP3 and calcitonin receptor-like receptor (CR

49 d mice with targeted deletions of either the RAMP2 or RAMP3 gene.

50 Co-expression of CRLR with RAMP2 or RAMP3 resulted in a response with the pharmacol

51 The interaction of RAMP2 or RAMP3 with CLR induces conformational variation

52 ion of CRLR when co-expressed with RAMP1 and RAMP2 or RAMP3, respectively, intermedin represents a no

53 otein-coupled receptor, paired with a RAMP1, RAMP2, or RAMP3 accessory subunit, respectively, which i

54 upted when P321A was coexpressed with RAMP1, RAMP2, or RAMP3.

55 e observed that AM-induced activation of CLR*RAMP2 promoted ubiquitination of CLR.

56 Because RAMP2, RAMP3, and CLR transduce the signaling of the two

57 intact CTR, AMY1 (CTR.RAMP1), and AMY2 (CTR.RAMP2) receptors using purified CTR extracellular domain

58 The discovery that RAMP2 regulates MRGPRX4 may have direct implications for

59 cells express the putative ADM-receptor CRLR-RAMP2 the production and secretion of ADM with the conse

60 RAMP2-transported receptors are core-glycosylated and ar

61 Degradation of CLR*RAMP2 and CLRDelta9KR*RAMP2 was not dependent on the duration of AM stimulatio

62 Degradation of CLR*RAMP2 was sensitive to overexpression of hepatocyte grow