ライフサイエンスコーパス: RAPD

1 RAPD analysis demonstrated a 1.4-kb band in all O19 stra

2 RAPD and PFGE showed that the 24 strains were a genetica

3 RAPD as measured in log units significantly correlated w

4 RAPD fingerprints obtained from primary culture plate co

5 RAPD fingerprints were much more informative than the pl

6 RAPD genotyping significantly correlated with resistance

7 RAPD marker analysis is more reliable and provides highe

8 RAPD markers cosegregated into 12 different S. brevidens

9 RAPD markers showed an overall bias toward vitripennis a

10 RAPD size correlated significantly with both RNFL thickn

11 RAPD type A was also isolated from the rubber liners of

12 RAPD was measured by the swinging flashlight method usin

13 RAPD-PCR demonstrated identical patterns among M. chimae

14 RAPD-PCR polymorphisms at 57 presumptive loci were used

15 RAPD-PCR polymorphisms were used to examine breeding str

16 RAPD-SSCP analysis revealed segregation of codominant al

17 RAPDs indicate that L. robustum ssp. walkeri in Sri Lank

18 in A aygpti, 94 markers were mapped with 10 RAPD primers in five weeks.

19 etailed map was constructed that includes 10 RAPDs.

20 ChxA locus (cycloheximide resistance) and 11 RAPDs (randomly amplified polymorphic DNAs).

21 n B. hebetor, 79 markers were mapped with 12 RAPD primers in six weeks; in A aygpti, 94 markers were

22 by the RAPD fingerprinting method alone, 14 RAPD subtypes were revealed.

23 quence-tagged site markers was initiated: 18 RAPD markers were cloned and sequenced, and single-stran

24 Out of the 20 RAPD markers, 19 were polymorphic, while OPA-03 was the

25 Polymorphisms were scored at 47-48 RAPD loci in each species.

26 Genotypes were also scored at 57 RAPD-SSCP loci, 5 (TAG)(n) microsatellite loci, and 6 se

27 The map includes 71 RAPD markers and one phenotypic marker, blonde.

28 ntains 79 markers (six microsatellite and 73 RAPD markers) in 21 linkage groups and spans over 953.1

29 y nullisomic mapping, does show linkage to a RAPD not yet assignable to chromosomes by nullisomic map

30 AFLP-, RAPD-, and RFLP-derived markers were used to saturate th

31 A total of 347 loci (AFLPs, RAPDs, and protein-coding loci) were mapped using an int

32 An RAPD was observed in 9% to 82% of patients with glaucoma

33 ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with

34 between South American classes 5 and 6, and RAPD-PCR showed 18 distinct genetic fingerprints in 20 i

35 0.320), demonstrating the value of AFLP and RAPD analyses in the characterization of disease-causing

36 Both AFLP and RAPD separated the isolates into two distinct groups, bu

37 c linkage map composed of 841 SSR, AFLP, and RAPD markers and phenotypic data from 310 progeny were u

38 on of Fst estimates across both allozyme and RAPD loci.

39 ARDRA and RAPD data showed three distinct lactobacilli strains, in

40 ation of bulked segregant analysis (BSA) and RAPD techniques.

41 study used quantitative characteristics and RAPD markers to examine the molecular and morphological

42 or a close relative of CGA (by ERIC2 PCR and RAPD analysis, respectively) and yielded a positive assa

43 PFGE and RAPD fingerprinting are best suited to addressing small-

44 r of the methods was variable, with PFGE and RAPD typing having a higher index of discrimination than

45 ethods employed, including PT, RT, PFGE, and RAPD analysis.

46 on, worse visual acuity on presentation, and RAPD on presentation.

47 presence of DNase, antibiotic resistance and RAPD profiles indicated that transfer was unidirectional

48 . coli with similar levels of resistance and RAPD-genotypes on days 0 and 42.

49 A total of 125 RFLP and RAPD markers were mapped into 117 different loci on nine

50 Ribotyping and RAPD clearly demonstrated the household transmission of

51 ere indistinguishable by both ribotyping and RAPD.

52 were less discriminatory than ribotyping and RAPD.

53 d M. szulgai strain with a gene sequence and RAPD pattern identical to those of the pseudoepidemic st

54 CYP51A sequencing and RAPD genotyping was performed.

55 e as dominant alleles, (2) that genotypes at RAPD loci are in Hardy-Weinberg proportions, (3) identit

56 Relationships between RAPD and RNFL thickness difference/ratio between the two

57 Both RAPD and ERIC genotyping methods had no correlation with

58 shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses.

59 suming (1) that genomic regions amplified by RAPD-PCR segregate as dominant alleles, (2) that genotyp

60 dom association between loci demonstrated by RAPD analysis and MLEE provide evidence for strong linka

61 The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs wit

62 f one of the three clusters discriminated by RAPD analysis, MLEE, and Ca3 fingerprinting, supporting

63 concordant with the genotypes identified by RAPD analysis, MLEE, and Ca3 Southern hybridization, res

64 ith a mixture of seven strains identified by RAPD-polymerase chain reaction, H. pylori Lewis expressi

65 breast tissues from the same individuals by RAPD (random amplified polymorphic DNA)/AP-PCR (arbitrar

66 olates closely resembled chicken isolates by RAPD and PFGE analysis.

67 that were of low intensity were observed by RAPD analysis, making interpretation more difficult.

68 Thirty types were observed by RAPD analysis.

69 rogram prepared from the results obtained by RAPD analysis, the isolates from the United States and E

70 were generally different from each other by RAPD analysis.

71 he large number of polymorphisms revealed by RAPD-PCR allowed the distribution of FST and linkage dis

72 mportant Aspergillus species was screened by RAPD-PCR to identify section- or species-specific amplic

73 , and inconclusive when comparing strains by RAPD-PCR.

74 PFGE gave evaluable patterns when studied by RAPD-PCR, with isolate clustering being consistent with

75 re resolved into three different subtypes by RAPD analysis.

76 early distinguished from Indian Ligustrum by RAPDs.

77 The process of converting RAPD markers to sequence-tagged site markers was initiat

78 study demonstrates the efficacy of coupling RAPD and HRS for a rapid and efficient screening of the

79 fluences the relative afferent pupil defect (RAPD).

80 .034), a relative afferent pupillary defect (RAPD) (P = .002), or a history of systemic hypertension

81 cause a relative afferent pupillary defect (RAPD).

82 ts with relative afferent pupillary defects (RAPDs) confirmed using the swinging flashlight method (m

83 entified similar subtle genomic differences, RAPD analysis provided only 77% of the detail provided b

84 However, the differentiated RAPDs and mtDNA were concordant in dividing the populati

85 A total of 62 distinct RAPD types were categorized from the five genera studied

86 th data from random amplified polymeric DNA (RAPD) and Penner serotyping analyses.

87 re done by random amplified polymorphic DNA (RAPD) analysis and amplified fragment length polymorphis

88 s based on random amplified polymorphic DNA (RAPD) analysis and multilocus enzyme electrophoresis (ML

89 gens using random amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFG

90 Randomly amplified polymorphic DNA (RAPD) analysis generated DNA profiles that clustered the

91 obes using random amplified polymorphic DNA (RAPD) analysis revealed genetic similarities and differe

92 , based on random amplified polymorphic DNA (RAPD) analysis was developed for its efficient and accur

93 C2 PCR and random amplified polymorphic DNA (RAPD) analysis, a PCR assay incorporating the new primer

94 bjected to random amplified polymorphic DNA (RAPD) analysis, and the data were analysed phenetically.

95 Randomly amplified polymorphic DNA (RAPD) analysis, multilocus enzyme electrophoresis (MLEE)

96 g methods: random amplified polymorphic DNA (RAPD) analysis, sequence-specific DNA primer (SSDP) anal

97 ugh random amplification of polymorphic DNA (RAPD) analysis.

98 AFLP), and random amplified polymorphic DNA (RAPD) analysis.

99 at couples Random Amplified Polymorphic DNA (RAPD) and a high-resolution capillary electrophoresis sy

100 xamined by random amplified polymorphic DNA (RAPD) and distinctive lactobacilli were sequenced.

101 ysed using random amplified polymorphic DNA (RAPD) and mtDNA RFLPs markers.

102 other by randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism analy

103 CR-based randomly amplified polymorphic DNA (RAPD) assay as well as to pulsed-field gel electrophores

104 an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Sile

105 of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were

106 A random amplified polymorphic DNA (RAPD) fingerprinting method has been developed to differ

107 Randomly amplified polymorphic DNA (RAPD) fingerprints generated from a single primer correc

108 mined with randomly applied polymorphic DNA (RAPD) markers in a second backcross population derived f

109 f the nine randomly applied polymorphic DNA (RAPD) markers we examined were more strongly differentia

110 s, using randomly amplified polymorphic DNA (RAPD) markers, conclusively showed that somatically comp

111 s of 122 randomly amplified polymorphic DNA (RAPD) markers, six molecular markers representing identi

112 cted using random amplified polymorphic DNA (RAPD) markers.

113 kers and randomly amplified polymorphic DNA (RAPD) markers.

114 y of the randomly amplified polymorphic DNA (RAPD) method to uncover genetic variation in this highly

115 A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of ad

116 reas the randomly amplified polymorphic DNA (RAPD) patterns of all nonpigmented strains were identica

117 s by using random amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) cloning and the TaqMan LS50B fluoro

118 PFGE by randomly amplified polymorphic DNA (RAPD) PCR, including isolates from eight nosocomial outb

119 Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Te

120 iii) the randomly amplified polymorphic DNA (RAPD) technique, and (iv) pulsed-field gel electrophores

121 and random amplification of polymorphic DNA (RAPD) type-specific differences in gluconate production,

122 inting and random amplified polymorphic DNA (RAPD) typing.

123 sis (MEE), random amplified polymorphic DNA (RAPD), and single-strand conformation polymorphism-were

124 ping (EK), random-amplified polymorphic DNA (RAPD), and the CNRE-1 DNA probe, MICs of amphotericin B,

125 sed random amplification of polymorphic DNA (RAPD), have already been utilized as powerful tools to d

126 ted by the random amplified polymorphic DNA (RAPD), multilocus enzyme electrophoresis (MLEE), and Ca3

127 ing random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE), and DNA-

128 by Random Amplification of Polymorphic DNA (RAPD)-PCR fingerprinting was used to group the genotypes

129 ared using random amplified polymorphic DNA (RAPD)-PCR typing.

130 by random amplification of polymorphic DNA (RAPD)-polymerase chain reaction (PCR).

131 (ERIC) and Random Amplified Polymorphic DNA (RAPD).

132 as random amplification of polymorphic DNA (RAPD)] and isozyme mapping resulted in the production of

133 CR-based randomly amplified polymorphic DNA (RAPD-PCR) method.

134 A, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AF

135 LPs) and randomly amplified polymorphic DNA (RAPDs), the methods of data analysis can be split into t

136 arkers and random amplified polymorphic DNA (RAPDs).

137 E], random amplification of polymorphic DNA [RAPD], and amplified fragment length polymorphism [AFLP]

138 a genetically diverse population with eight RAPD profiles.

139 ted primer sets are identified and employed, RAPD analysis provides a simple, rapid, and powerful sub

140 ica, from Mexico to Panama, and analysed for RAPD DNA variation.

141 ncordance of the results is demonstrated for RAPD analysis, MLEE, and Southern blot hybridization wit

142 The methodologies for RAPD typing, ribotyping, and PFGE typing of C. diphtheri

143 two known, noninbred parents was scored for RAPD and AFLP markers, in order to develop a linkage map

144 anisocoria produced a significant change in RAPD from baseline (mean = 1.60 dB in the miotic eye, P

145 A total of 32decamer primers were used in RAPD analysis; 19 of them provided at least one polymorp

146 To test EstA and AcpA for linkage to known RAPD loci on their respective chromosomes, a panel of Ro

147 markers were consistent with those of linked RAPD markers.

148 specificity and the lack of codominance make RAPD markers less practical for mapping than microsatell

149 ci are employed (eg 50 loci, typical of many RAPD studies).

150 rase chain reaction-based molecular markers (RAPDs).

151 the samples were screened with eight 10-mer RAPD primers, a total of 12 polymorphic bands were detec

152 Moreover RAPD markers have been applied in order to describe thei

153 Moreover, RAPDs showed that introduced material in La Reunion has

154 nalyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing

155 d several weeks later was caused by multiple RAPD types, which rules out contagious transmission and

156 Nevertheless, RAPD-PCR allowed recording a relevant genotypic biodiver

157 The diagnostic accuracy of RAPD was inferior to the RAPDx and is likely related to

158 conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps in a haplodiploid

159 The analysis of RAPD products with HRS showed differences between the pr

160 SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of

161 2% and a specificity of 94% for detection of RAPD whereas the binocular OCT had a sensitivity of 74%

162 The magnitude of RAPD was calculated using the log of the ratio of the co

163 The discriminatory power of RAPD analysis was slightly lower (D = 0.971), whereas SS

164 poor postoperative outcome were presence of RAPD (p = .014), wound extending into zone III (p = 0.02

165 Better detection of RAPDs may be one way to identify persons with glaucoma.

166 ere identified using a combined approach of (RAPD)-PCR and 16S rRNA gene sequencing.

167 With the OPZ19 RAPD primer a 1,264-bp product was amplified from all A.

168 No changes in MEE or RAPD subtypes were detected in serial isolates from any

169 ither testing on CHROMagar Candida plates or RAPD fingerprinting.

170 ne-third as many distinct subtypes as REA or RAPD analysis; the Singaporean isolates were distributed

171 to interpret than those obtained by RFLP or RAPD analysis.

172 integration genes were present in two other RAPD types.

173 of all 4 coincided with those of the parent RAPD markers.

174 A pathological RAPD was above +/-0.5 log units on both devices.

175 ofile and random arbitrarily primed DNA PCR (RAPD-PCR) profiles of the progeny indicated that DNA tra

176 random amplification of polymorphic DNA-PCR (RAPD-PCR).

177 random amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) cloning and the TaqMan LS50B fluorogenic detec

178 Linkage analysis of AP-PCR/RAPD and isozyme markers resulted in the addition of 16

179 nal resolution and reproducibility of AP-PCR/RAPD generated markers.

180 horus gene map by linkage analysis of AP-PCR/RAPD markers in conjunction with isozyme polymorphisms s

181 Addition of 5 AP-PCR/RAPD markers to linkage group U6 containing the Tailspot

182 n randomly chosen and characterized by PFGE, RAPD, and AFLP.

183 ly chosen and subjected to analysis by PFGE, RAPD, and AFLP.

184 by a single strain of Klebsiella pneumoniae, RAPD type A, which was detected in milk from eight cows.

185 en decamer primers generated 102 polymorphic RAPD bands and pairwise distances were calculated betwee

186 phic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated lin

187 d polymorphic DNA polymerase chain reaction (RAPD-PCR).

188 Six RAPD types and 15 PFGE patterns were identified among al

189 ast one, but not all, synteny group-specific RAPD markers.

190 We constructed a joint SSR/RAPD genetic linkage map of the Helianthus petiolaris ge

191 These findings suggest RAPD-PCR typing can distinguish unique CF isolates of S.

192 ) microsatellite loci, and 6 sequence-tagged RAPD loci.

193 PFGE with SpeI was more reproducible than RAPD and BOX-PCR fingerprinting.

194 This study demonstrates that RAPD-PCR can be used for genetic comparison of M. absces

195 We propose that RAPD and AFLP provide valuable tools for molecular typin

196 The RAPD data revealed within-section interspecies relations

197 The RAPD method appears useful for the construction of a gen

198 The RAPD profiles of four isolates obtained were again ident

199 The RAPD technique was adequate for biotype differentiation;

200 The RAPD-SCAR technique is here described, for the first tim

201 However, by the RAPD fingerprinting method alone, 14 RAPD subtypes were

202 ts minor differences were seen in either the RAPD genotype or the microsatellite allele composition d

203 All 10 primers used for the RAPD analyses can distinguish C. fetus strains of reptil

204 and Critical Care Center were involved, the RAPD assay revealed that all 20 isolates obtained from c

205 d in a shorter time, the introduction of the RAPD assay into hospital microbiology laboratories as a

206 Approximately a third of the RAPD markers analysed segregated in both the 87-25-1M x

207 the potential influence of anisocoria on the RAPD and also greater susceptibility of lightly pigmente

208 ll 25 linkage groups have been mapped on the RAPD zebrafish genetic map.

209 Since the RAPD assay could provide discriminatory potential and re

210 mmon source (the pseudoepidemic strain), the RAPD patterns of the other strains were varied.

211 Thus, our experience demonstrated that the RAPD assay is a useful and reliable tool for epidemiolog

212 electrophoretic karyotyping showed that the RAPD fingerprint was correct.

213 The main outcome measure was the RAPD, determined by computerized pupillography, at basel

214 he microsatellite-based genetic map with the RAPD map to identify markers most likely linked to the c

215 Molecular typing with the RAPD technique was useful in discriminating between pati

216 AcpA (on 3R), while unlinked to all the RAPDs assigned to chromosome 3 by nullisomic mapping, do

217 These RAPD data enabled us to pinpoint a possible source and t

218 These RAPD markers permit the PCR amplification of a DNA speci

219 This RAPD approach to S. enteritidis subtyping provided more

220 This RAPD-PCR and TaqMan assay offers promise as a nucleic ac

221 The bacterial DNA used in this RAPD protocol was obtained by boiling the bacterial samp

222 According to RAPD analysis, PDT-3-1-1 and PB Keshri have the highest

223 nd anthocyanin spotting (Rs)] were linked to RAPD loci, but only six of them could be placed on an ex

224 owed perfect amplification when subjected to RAPD, restriction digestion and amplification with DNA b

225 Twenty RAPD primers were used in molecular characterisation, pr

226 in (ctx) genes were found in isolates of two RAPD types, in each case embedded in CTXphi-like prophag

227 ntrogression in this tree was detected uisng RAPD markers.

228 First, a linkage map was produced using RAPD markers.

229 trains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gen

230 .3% direct concordance with MLEE, 82.7% with RAPD analysis, and 86.2% with the Ca3 Southern hybridiza

231 No new cases with RAPD type A were observed after implementation of interv

232 However, anisocoria correlated with RAPD only in subjects with darkly pigmented irides (Pear