ライフサイエンスコーパス: REDOR

1 REDOR indicated that phosphorus was still distant, but n

2 REDOR NMR and synthetic studies established the T-taxol

3 REDOR NMR studies of a 151 kDa complex of uniformly 15N-

4 REDOR revealed that the phosphorus of PEP was cleaved.

5 REDOR-constrained docking simulations indicate that thes

6 ge deformations upon ligand binding, 31P-19F REDOR measurements can also serve as an assay for comple

7 We have performed solid-state 31P-19F REDOR nuclear magnetic resonance (NMR) experiments to mo

8 These 31P-19F REDOR results demonstrate the ability of solid-state NMR

9 we present experimental results from 2H{19F} REDOR NMR that provide direct confirmation that paclitax

10 The 15N{31P} REDOR NMR spectra of three metabolically stable phosphon

11 positions that agree with the type-assigned REDOR dephasing.

12 sult suggests that the K3 dimers detected by REDOR at L/P = 20 are not on the surface of the bilayer

13 luorine and carbon nuclei were determined by REDOR.

14 ical distance has been precisely measured by REDOR solid-state NMR spectroscopy in the transmembrane

15 Interhelical (15)N...(13)C REDOR measurements between Gln664 side chains on opposin

16 enrichment, and measured it with (19)F-(13)C REDOR.

17 (15)N{(13)C} REDOR measurements are consistent with a single (13)C-(1

18 d heteronuclear spin pair under conventional REDOR.

19 CP-REDOR NMR reveals that each Asp is embedded in a perturb

20 NMR, CP rotational-echo double resonance (CP-REDOR) NMR, and heteronuclear correlation (HETCOR) NMR s

21 from a new solid-state NMR experiment, DANTE-REDOR, which can determine global secondary structure in

22 simulations of binding restrained by double REDOR-determined intramolecular C-F distances.

23 ble rotational-echo double resonance (double REDOR) has been used to investigate the bound conformati

24 ble rotational-echo double resonance (double REDOR) NMR was used to investigate the conformation of a

25 ar dynamics simulations restrained by double-REDOR-determined intramolecular (13)C-(19)F distances re

26 g a new solid-state NMR technique called DSQ-REDOR, are consistent with hydrogen bonds between side c

27 Full-echo REDOR spectra of cell-wall complexes from cells labeled

28 This efficient REDOR simulation is combined with simulated annealing to

29 tance measurement approach, using (13)C(19)F REDOR, to measure a ligand-induced change of 1.0 +/- 0.3

30 sive peptide-headgroup contact, (13)C[(19)F] REDOR experiments on MLVs containing specifically (19)F-

31 Complementary (31)P[(19)F] REDOR experiments on these MLVs show an enhanced headgro

32 lely on results for epimer B, a (15)N[(19)F] REDOR NMR study was performed on the complex formed from

33 olar couplings were measured by (13)C[(19)F] REDOR.

34 obtained from (13)C{(19)F} and (15)N{(19)F} REDOR dephasing allow a correlation of structure and ant

35 The (13)C{(19)F} REDOR dephasing maximum indicates that the secondary bin

36 (15)N{(19)F} REDOR dephasing shows the proximity of the fluorine to t

37 The (13)C{(19)F} and (15)N{(19)F} REDOR spectra show that, in situ, DFPBV binds to the pep

38 yostatin analogues strategically labeled for REDOR NMR analysis.

39 ogues were synthesized, and, as required for REDOR analysis, all proved highly potent with PKC affini

40 e of positions found by this assignment-free REDOR approach.

41 From REDOR dipolar recoupling data, the structure and orienta

42 X-Pro peptide bonds in bR are assigned from REDOR difference spectra of pairwise labeled samples, an

43 he distance and orientation information from REDOR is consistent with a parallel (N-N) dimer structur

44 ing calculated to experimental (13) C{(1) H} REDOR and (13) C chemical shift anisotropy (CSA) tensor

45 urements, (27) Al MAS NMR and (27) Al{(1) H} REDOR experiments, and computational spectroscopy reveal

46 uclear correlation spectra, and (87)Rb{(1)H} REDOR spectra.

47 However, REDOR NMR requires strategically labeled, high affinity

48 ed question mark(19)F inverted question mark REDOR with natural-abundance background interferences re

49 ed question mark(15)N inverted question mark REDOR.

50 We simulate the measured REDOR dephasing using a second-moment approximation with

51 (17)O-(15)N REDOR curves are presented for glycine.(2)HCl; fits of t

52 ll-echo spectra were removed by (13)C[(15)N] REDOR.

53 In contrast, both (13)C{(15)N} REDOR and (13)C CPMAS are consistent with multiple (13)C

54 The (13)C{(15)N} REDOR experiments resonant at 165 ppm show an incomplete

55 ouble-resonance (REDOR) NMR and (13)C{(15)N} REDOR to determine the chemical identity of these produc

56 In the present study, solid-state NMR REDOR methods were applied for detection of oligomeric b

57 Comparisons of simulated and observed REDOR dephasing were used to deduce a closest approach d

58 f the His37 side chain based on the observed REDOR distance.

59 A combination of REDOR, rotational-echo double-resonance, and conventiona

60 Comparison of REDOR spectra on samples that were labeled at different

61 Based on REDOR results, we determined that cheatgrass (a plant th

62 This model is also consistent with six other REDOR-determined internuclear distances, most of which a

63 Our REDOR experiments indicate that alpha-helical Ala19-maga

64 ues (Ala(46) and Ala(49)) using (13)C{(31)P} REDOR and one lysine residue (Lys(52)) using (15)N{(31)P

65 lysine residue (Lys(52)) using (15)N{(31)P} REDOR.

66 We performed REDOR solid-state NMR experiments on lyophilized samples

67 measurements suggesting that the present PTX REDOR distances may not provide a precise model for bioa

68 )Nb{(31)P} rotational echo double resonance (REDOR) and, for the first time, (31)P{(93)Nb} rotational

69 restingly, Rotational Echo DOuble Resonance (REDOR) difference spectroscopy of [20-13C]retinal,[indol

70 1)B{(31)P} rotational echo double resonance (REDOR) experiments show systematic deviations from calcu

71 contrast, rotational echo double resonance (REDOR) NMR experiments revealed that the sequence Ala24-

72 using the rotational echo double resonance (REDOR) NMR method.

73 Rotational-echo double resonance (REDOR) NMR provided internuclear distances from the 19F

74 Rotational echo double resonance (REDOR) NMR spectroscopy could uniquely address this chal

75 17O-1H rotational echo double resonance (REDOR) NMR was applied to probe the O-H distances in zeo

76 DR-CT) and rotational echo double resonance (REDOR) solid-state NMR techniques, demonstrate that octa

77 DRAMA) and rotational-echo double resonance (REDOR) to determine intra- and interligand internuclear

78 technique, rotational-echo double resonance (REDOR), can be used to measure both intra- and intermole

79 3)C{(15)N} Rotational Echo DOuble Resonance (REDOR), the structure of the C-terminus was found to be

80 3)C{(31)P} rotational echo double resonance (REDOR)NMR experiments reveal coprecipitated phosphate th

81 Rotational-echo double-resonance (REDOR) (13)C[(31)P] and (15)N[(31)P] experiments on the

82 Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been ob

83 3)C{(19)F} rotational-echo double-resonance (REDOR) dephasing for the cell-wall (13)C-labeled bridgin

84 sured in a rotational-echo double-resonance (REDOR) experiment performed on mixtures of differently l

85 Rotational-echo double-resonance (REDOR) experiments on [15N]Ser bound to a [1-13C]Phe-rec

86 3)C{(19)F} rotational-echo double-resonance (REDOR) experiments on whole cells enriched with l-[1-(13

87 (19)F-(1)H rotational-echo double-resonance (REDOR) experiments.

88 13)C/(15)N rotational echo double-resonance (REDOR) measurements indicate an antiparallel organizatio

89 5)N{(13)C} rotational-echo double-resonance (REDOR) NMR and (13)C{(15)N} REDOR to determine the chemi

90 1)Ga{(1)H} rotational-echo double-resonance (REDOR) NMR and other data indicate that PVA achieves opt

91 S-echo and rotational-echo double-resonance (REDOR) NMR experiments, employing a slow catalytic EPSP

92 1)P[(19)F] rotational-echo double-resonance (REDOR) NMR measurements, we establish that UDG partially

93 onance and rotational echo double-resonance (REDOR) NMR methods.

94 fforded by rotational-echo double-resonance (REDOR) NMR to interrogate (13) C-(1) H distances is expl

95 olid state rotational-echo double-resonance (REDOR) NMR was used to probe the internuclear distance b

96 ermined by rotational-echo double-resonance (REDOR) NMR.

97 The rotational-echo double-resonance (REDOR) pulse sequence was used to measure the internucle

98 -selective rotational-echo double-resonance (REDOR) solid-state NMR experiment to measure the concent

99 thors used rotational-echo double-resonance (REDOR) solid-state NMR to measure intermolecular and int

100 13)C-(19)F rotational-echo double-resonance (REDOR) spectra that probe protein-ligand proximities and

101 using the rotational-echo double-resonance (REDOR) technique under magic-angle spinning.

102 Fast MAS (1)H{(195)Pt} dipolar-HMQC and S-REDOR experiments were implemented on both the molecular

103 (13) C{(27) Al} RESPDOR and (1) H{(19) F} S-REDOR experiments are consistent with a weakly coordinat

104 ce (1)H{(133)Cs} RESPDOR and (1)H{(207)Pb} S-REDOR experiments.

105 experiment, termed FSR (frequency selective REDOR), combines the REDOR pulse sequence with a frequen

106 sed on the interpretation of two solid-state REDOR (13)C-(19)F distances in a fluorinated PTX derivat

107 The REDOR results indicate a single fluorine dephasing cente

108 The REDOR spectra show that aromatic carbons from the polyst

109 The REDOR technique allows (19)F...(31)P distances to be mea

110 SR (frequency selective REDOR), combines the REDOR pulse sequence with a frequency selective spin-ech

111 ng restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptopha

112 no significant dephasing was observed in the REDOR experiment in the dark or upon light activation.

113 In the REDOR measurements, observation of reduced (13)C intensi

114 at 165 ppm show an incomplete buildup of the REDOR data to approximately 90% of the expected maximum.

115 Simulation of the REDOR dephasing curves suggests up to ~60 % FA(+) incorp

116 d torsions and a justifiable increase of the REDOR distance error to > or = +/-0.7 A readily resolves

117 This study demonstrates the utility of the REDOR NMR technique for the elucidation of the oligomeri

118 (13)C-(19)F separations compatible with the REDOR measurements suggesting that the present PTX REDOR

119 etastable state that are consistent with the REDOR NMR measurements.

120 odel of the binding site consistent with the REDOR results positions the vancomycin cleft around an u

121 ar dynamics simulations constrained by three REDOR-determined distances to His156.

122 We also use REDOR to measure dipolar interactions in [20-13C]retinal

123 roposed covalent intermediates, we have used REDOR to measure the same distances in enzyme-free and e

124 determine the single 31P-31P distance, while REDOR was used to determine one 31P-15N distance and fiv

125 and (31)P spins in the crystal surface with REDOR NMR show that, in the peptide fragment derived fro