ライフサイエンスコーパス: RFP

1 RFP expression was also shown by flow cytometry and repl

2 RFP expression was analyzed by flow cytometry and viral

3 RFP insertion marked putative pax8-lineage cells with fl

4 RFP involved eye exams, dilation and 40-degree fundus ph

5 RFP is expressed in the dorsal spinal cord.

6 RFP was able to repress transcriptional activation by E4

7 RFP-FimXDeltaREC, unlike RFP-FimX, is no longer localize

8 RFP-GRalpha receptor behaves similarly to the wild-type

9 RFP-GRalpha translocation was temperature sensitive, occ

10 RFP-Luc-Sk-Hep-1 were implanted into NOD-scid mice liver

11 including PC-3-RFP prostate cancer, HCT-116-RFP colon cancer, MDA-MB-435-RFP breast cancer, and HT10

12 Using IL-17F-RFP together with a Foxp3 reporter, we found that the de

13 The AtSRC2.2-RFP fusion also colocalized with two proteins previously

14 MIA-PaCa-2-RFP pancreatic cancer cells were transduced with the Dis

15 g signal 1 and peroxisome targeting signal 2-RFP in transgenic Arabidopsis.

16 sing human cancer cell lines, including PC-3-RFP prostate cancer, HCT-116-RFP colon cancer, MDA-MB-43

17 cancer, HCT-116-RFP colon cancer, MDA-MB-435-RFP breast cancer, and HT1080-RFP fibrosarcoma were tran

18 ells and M2 cells transfected with filamin A-RFP), caveolin-1-GFP was concentrated in intracellular v

19 Sla1-GFP and Abp1-RFP lifetimes were accelerated in arp2-7 mutants, which

20 imaging of Sla1-GFP, a coat marker, and Abp1-RFP, which marks the later actin phase of endocytosis.

21 larization process in vitro by using an ActA-RFP (red fluorescent protein) fusion.

22 Adding RFP to community eye clinics was associated with an incr

23 autophagy, neurons were infected with adeno-RFP-LC3 and subjected to trophic factor withdrawal, and

24 expression of Acan and Runx2 induced by Adv-RFP-LOXL2 was higher in females compared to males.

25 Interestingly, Adv-RFP-LOXL2 injection significantly increased Rankl expres

26 ales, as compared to those injected with Adv-RFP-Empty in respective groups.

27 jected to intraperitoneal injection with Adv-RFP-LOXL2 every 2 weeks for 12 weeks.

28 ferent patterns of gene expression: alphaSMA-RFP positive cells, collagen-EGFP positive cells, and ce

29 The alphaSMA-RFP mice were crossed with collagen-EGFP mice to generat

30 h rabbit myocardium and in mice harboring an RFP-GFP-LC3 transgene.

31 Standard care and RFP clusters were compared by the proportion of patients

32 reB, co-visualization of GFP-tagged DnaA and RFP-tagged MreB demonstrates that DnaA and MreB adopt di

33 is, histochemical GUS staining, and eGFP and RFP fluorescent microscopy.

34 conditions, nephrons expressed few EGFP and RFP puncta, but ischemia-reperfusion injury (IRI) led to

35 ete release of constituent proteins, GFP and RFP (mCherry), from a polyprotein precursor, in bacteria

36 el fluorescent proteins derived from GFP and RFP in an in vitro system that allows direct analysis of

37 cellular measurements, as shown for GFP and RFP in mammalian cells.

38 By revealing how GFP and RFP protein environments steer chemistry to favor fluoro

39 showed rapid spatial overlap between GFP and RFP signal at the site of fusion.

40 e light fluorescent proteins such as GFP and RFP suffers from poor tissue penetration and high backgr

41 d was produced in recombinant form (His- and RFP-tagged).

42 t recruitment of RFP approximately KLCR1 and RFP approximately CaM2 to microtubules.

43 Interestingly, while GFP-Wdr68 and RFP-Dyrk1a co-localized to the cell nucleus as expected

44 actions and localization of Apc2-GFP and Apc-RFP to branch points suggests that these proteins work t

45 Transient overexpression of ARK1-RFP (red fluorescent protein) increased microtubule cata

46 ute [mRNA] concentration and the associated [RFP] expressed from an inducible plasmid.

47 an cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

48 h one of the following constructs: RCASBP(B)-RFP, RCASBP(B)-RFP-scrambled RNAi, or RCASBP(B)-RFP-GluA

49 , RCASBP(B)-RFP-scrambled RNAi, or RCASBP(B)-RFP-GluA2 RNAi.

50 llowing constructs: RCASBP(B)-RFP, RCASBP(B)-RFP-scrambled RNAi, or RCASBP(B)-RFP-GluA2 RNAi.

51 in the ND-GFP mice transplanted with B16F10-RFP.

52 and bone marrow neutrophils (Nphs) from BAFF-RFP mice expressed the highest constitutive levels of BA

53 Treatment of BAFF-RFP mice with polyinosinic:polycytidylic acid increased

54 FF functions, we created BAFF reporter (BAFF-RFP) mice and Baff floxed (Baff(fl/fl) ) mice.

55 ronment of the autolysosomes, whereas bright RFP signals remained.

56 that some Rim20-GFP foci correspond to Bro1-RFP foci, whereas others do not.

57 s having both Ure2p-GFP aggregates and Btn2p-RFP dots display striking colocalization.

58 ation of rKSHV.219 occurred, as evidenced by RFP expression, the expression of the late virion protei

59 Transcriptional repression by RFP was trichostatin A sensitive and did not involve an

60 nt protein (EGFP; pKa 5.9), we generated CAG-RFP-EGFP-LC3 mice to distinguish early autophagic vacuol

61 t deprivation, renal epithelial cells in CAG-RFP-EGFP-LC3 mice produce autophagic vacuoles expressing

62 h genetically color-coded macrophages (Ccr2 (RFP)) and microglia (Cx3cr1 (GFP)).

63 absence of phagocytizing macrophages (Ccr2 (RFP/RFP)), microglia are effector cells of tumor cell ph

64 C57BL/6J, Ccr2(RFP/+)Cx3cr1(GFP/+), Ikk(F/F) mice and LysM-Cre/Ikk(F/F)

65 ing T. gondii infection of C57BL/6J and CCR2(RFP/+)CX3CR1(GFP/+) mice.

66 Using CX3CR1(GFP/+)CCR2(RFP/+) reporter mice, we show that TBI initiated a tempo

67 roglia before tMCAO in P9 Cx3cr1(GFP/+)/Ccr2(RFP/+) mice exacerbated injury and induced hemorrhages a

68 cts were independent of infiltration of Ccr2(RFP/+) monocytes into injured regions.

69 dels in combination with CX3CR1(GFP/WT);CCR2(RFP/WT) double knock-in mice.

70 ption unit activation in Cx3cr1(+/GFP)CCR2(+/RFP) knockin fluorescent protein reporter mice.

71 infiltrating peripheral myeloid cells (CCR2-RFP) and related changes in these cells to Ia synaptic l

72 n through cranial windows revealed that CCR2-RFP monocytes were recruited to the blood-brain barrier

73 in myeloid cells exist, among them the CCR2-RFP and the CX3CR1GFP mouse.

74 In plant cells, CHX20-RFP co-localized with an endoplasmic reticulum marker, w

75 LdSar1 in Ldgp63 trafficking, we coexpressed RFP-Ldgp63 along with LdSar1:WT-GFP or LdSar1:T34N-GFP a

76 proliferation decreased in cells containing RFP puncta, suggesting that autophagic cells are less li

77 rtment (IGC), with more glomeruli containing RFP(+)CoRL and, within these glomeruli, more RFP(+)CoRL.

78 ng approaches are discussed for conventional RFPs, far-red FPs, RFPs with a large Stokes shift, fluor

79 scence signals of all jellyfish GFP or coral RFP derivatives, respectively.

80 nant rMERS-CoV, rMERS-CoV*ORF5, and MERS-CoV-RFP replicated to high titers, whereas MERS-DeltaORF3-5

81 CXCR7 caused abnormal accumulation of CXCL12-RFP at CXCR4-positive sites in the nasal area of CXCL12-

82 4-positive sites in the nasal area of CXCL12-RFP-transgenic mice and excessive CXCL12-dependent intra

83 tural features in other maturation-deficient RFPs may result in RFPs with faster and more complete ma

84 red chromophore maturation in DsRed-derived RFPs.

85 econd group, VSV-CT1, VSV-dG-GFP, and VSV-dG-RFP, had significantly diminished toxicity toward normal

86 Ts and were encountered once per 44 s of EB3-RFP comet growth time with about 5 s half-lifetime.

87 The vectors express either RFP or GFP markers, allowing simple in vivo tracking of

88 w fluorescent mPlum mutant to a red-emitting RFP without reverting any of the mutations causing the m

89 pressing macrophages were observed engulfing RFP-expressing cancer cells.

90 These enhanced RFPs provide new possibilities to study biological proce

91 For most imaging experiments, RFPs that mature quickly to the red chromophore and prod

92 ascending (CiA) interneurons do not express RFP.

93 ed movement and fusion than cells expressing RFP-Htt protein with 28 polyglutamine repeats.

94 one expressing GFP and the other expressing RFP, were simultaneously injected in the inguinal lymph

95 mice produce autophagic vacuoles expressing RFP and EGFP puncta.

96 expression of Ruby2, a mutant of the red FP (RFP), is not affected.

97 iscussed for conventional RFPs, far-red FPs, RFPs with a large Stokes shift, fluorescent timers, irre

98 ersibly switch their fluorescent marker from RFP(+) to GFP(+) due to mesenchymal-specific Cre express

99 ations of red chromophores allows the future RFP phenotypes and their respective novel imaging applic

100 rs (agroinfection) to express functional GBP:RFP fusion (chromobody) in the model plant Nicotiana ben

101 Most interestingly, GBP:RFP can be applied to interfere with the function of GFP

102 eated with cyclophosphamide, the HCT-116-GFP-RFP cells also survived and formed colonies in the liver

103 ost cellular system attacked the HCT-116-GFP-RFP cells but could not effectively kill the MMT-GFP-RFP

104 The data suggest rapid death of HCT-116-GFP-RFP cells in the portal vein.

105 ruction of the cytoplasm) of the HCT-116-GFP-RFP cells occurred within 6 hours.

106 Most HCT-116-GFP-RFP cells remained in sinusoids near peripheral portal v

107 Human HCT-116-GFP-RFP colon cancer and mouse mammary tumor (MMT) cells wer

108 e mammary tumor (MMT) cells were HCT-116-GFP-RFP in the portal vein of nude mice.

109 protein (RFP) in the cytoplasm (HCT-116-GFP-RFP) were injected in either the PV or spleen of nude mi

110 In contrast, dual-color MMT-GFP-RFP cells injected into the portal vein mostly survived

111 Many surviving MMT-GFP-RFP cells showed invasive figures with cytoplasmic protr

112 s but could not effectively kill the MMT-GFP-RFP cells.

113 SV-CT1/CT9), G protein deletions (VSV-dG-GFP/RFP), and combinations thereof (VSV-CT9-M51).

114 as partially restored in the presence of GFP/RFP packaging constructs.

115 enuations included gene shifting (VSV-p1-GFP/RFP), M protein mutation (VSV-M51), G protein cytoplasmi

116 A total of 42 of 69 had RFP at rest, which reverted to non-RFP at stress in 24 (

117 er, MDA-MB-435-RFP breast cancer, and HT1080-RFP fibrosarcoma were transplanted to the transgenic GFP

118 e quest for further red-shifted and improved RFPs continues.

119 All clusters took part in RFP during the last step.

120 eased by 60%, 40%, and 49%, respectively, in RFP-positive Sk-Hep-1 recovered by fluorescence-activate

121 ther maturation-deficient RFPs may result in RFPs with faster and more complete maturation to the red

122 roaden the definition of BRCAness to include RFP and regulatory mechanisms that cause synthetic letha

123 over, RAAS inhibition in FSGS mice increased RFP(+)CoRL transdifferentiation in the IGC to phenotypes

124 ersely, inhibition of mTOR complex 1 induced RFP and EGFP expression and decreased cell proliferation

125 cation; however, 17AAG prevented DEX-induced RFP-GRalpha nuclear translocation.

126 oth nuclear import and export of DEX-induced RFP-GRalpha were faster than RU-486-induced nuclear shut

127 chicine blocked DEX-induced or RU486-induced RFP-GRalpha nuclear translocation; however, 17AAG preven

128 Instead, RFP(+) cells exhibited axons descending ipsilaterally, a

129 GnRH neurons expressing actin-GFP or Lifeact-RFP, calcium release was found to stimulate leading proc

130 u-NPs triggered autophagy in macrophage-like RFP-GFP-LC3 reporter cells, and cell death was aggravate

131 Comparisons with mKeima LSS RFP suggest that similar proton relays could be engineer

132 t proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level ex

133 nt protein (RFP)-labeled peroxisomal markers RFP-peroxisome targeting signal 1 and peroxisome targeti

134 ravital multiphoton microscopy of DC(GFP)/MC(RFP) reporter mice, we herein provide in vivo evidence t

135 he dominant negative effect of the monomeric RFP-ORF23 (mRFP23) fusion protein.

136 RFP(+)CoRL and, within these glomeruli, more RFP(+)CoRL.

137 ast, mCherry fused to an internal loop (MreB-RFP(SW)) does not induce helices.

138 tate, "patchy" localization patterns of MreB-RFP(SW), even by standard light microscopy.

139 ows microspore-specific activity and a MYB81-RFP fusion protein is only expressed in a narrow window

140 Myom2-RFP(+) PSC-CMs exhibited more mature phenotypes than RFP

141 resent feasibility of an in vivo assay (NFAT-RFP) that reports transcriptional activity of NFAT via e

142 Furthermore, NFAT-RFP can be used simultaneously with NFAT-GFP fusion prot

143 TOR), which prevents autophagy, contained no RFP puncta.

144 , but persisted in 18 (EE); 27 of 69 had non-RFP at rest and peak stress (AA).

145 of 69 had RFP at rest, which reverted to non-RFP at stress in 24 (EA), but persisted in 18 (EE); 27 o

146 lutamate receptors, the intensity of the NPY-RFP-labeled puncta declined in a step-like manner indica

147 ysosome fusion measured by colocalization of RFP-LC3 and LysoSensor Green was accelerated by IGF-I.

148 37 patients with an established diagnosis of RFP (the diagnosis was proven by biopsy in 31 patients),

149 Nuclear export of RFP-GRalpha was studied using confocal microscopy follow

150 Expression of RFP accounted for viral transduction efficiency and long

151 s at E2 resulted in widespread expression of RFP throughout the spinal cord with >/=60% of Islet1/2-p

152 Imaging of RFP-tagged end binding protein 1 (EB1) and YFP-tagged al

153 3 days after IRI, whereas the high levels of RFP puncta persisted, indicating autophagy initiation at

154 Nuclear localization of RFP-GRalpha in NTM5 cells treated with vehicle (ethanol)

155 ent discoveries, which include mechanisms of RFP regulation, DNA damage checkpoint proteins, as well

156 proximal tubules, with increased numbers of RFP and EGFP puncta that peaked at 1 day after IRI.

157 Time-lapse recordings of RFP-labeled stable synaptic sites demonstrate that Dkk1

158 approximately IQD1-dependent recruitment of RFP approximately KLCR1 and RFP approximately CaM2 to mi

159 ed the B-box and first coiled-coil region of RFP together with the bHLH domain of SCL.

160 hoton Ca(2+) imaging to map the responses of RFP-positive and neighboring L2/3 neurons to whisker def

161 re raised on the fidelity and sensitivity of RFP-to-GFP switch of this model in reporting EMT of meta

162 rossing to Rosa26:tdRFP mice, and sorting of RFP(+): glucagon(+) cells from KO mice, revealed recombi

163 retion of Ldgp63, whereas the trafficking of RFP-Ldgp63 in GFP-LdRab1:WT-expressing cells is unaltere

164 on had no effect on nuclear translocation of RFP-GRalpha.

165 cells showed robust nuclear translocation of RFP-STAT3 or STAT1-GFP, respectively.

166 The prognostic value of RFP at peak stress in ICM is unknown.

167 DNA coding for a non-fluorescent variant of RFP.

168 ch to red-shift the fluorescence emission of RFPs.

169 . maximus populations grown monoxenically on RFP labeled Escherichia coli in a soil slurry.

170 lusters) were randomized to standard care or RFP across five 2-week study periods (steps).

171 reproductive isolation, we expressed GFP or RFP in sperm heads of recently diverged sister species,

172 n (RFP) in the cytoplasm or with GFP only or RFP only were injected into the inguinal lymph node of n

173 cell-specific expression of CFP, GFP, YFP or RFP revealed the simultaneous migration of multiple podo

174 om embryonic day 12.5 (E12.5) and E13.5 Osr2(RFP/+) and Osr2(RFP/-) mutant mouse embryos and performe

175 12.5 (E12.5) and E13.5 Osr2(RFP/+) and Osr2(RFP/-) mutant mouse embryos and performed whole transcri

176 1.5-fold at both E12.5 and E13.5 in the Osr2(RFP/-) palatal mesenchyme cells, in comparison with Osr2

177 al mesenchyme cells, in comparison with Osr2(RFP/+) littermates.

178 VSV-M51, VSV-CT9-M51, VSV-p1-GFP, and VSV-p1-RFP.

179 Furthermore, P6-RFP coimmunoprecipitated with PDLP1-GFP.

180 We examined the colocalization of P6-RFP I-LBs with PDLP1-green fluorescent protein (GFP) and

181 observed at plasmodesmata) and found that P6-RFP I-LBs were associated with each of these markers.

182 termine whether restrictive filling pattern (RFP) at peak stress has prognostic value.

183 We first identified a circularly permuted RFP (cpRFP) scaffold, which maintained its autocatalytic

184 toactivatable and reversibly photoswitchable RFPs.

185 ed with shorter recurrence-free probability (RFP; P = .043), the relative proportion of stromal FoxP3

186 lude defects in replication fork protection (RFP).

187 for the first time that Ret finger protein (RFP), a member of the TRIM family of proteins initially

188 L-fucosidase and a red fluorescence protein (RFP) exhibited increased fluorescence intensity in media

189 e protein (GFP) or red fluorescence protein (RFP).

190 carrying the marker red fluorescent protein (RFP) and a GluA2 RNAi construct to downregulate GluA2 ex

191 017 with N-terminal red fluorescent protein (RFP) and C-terminal green fluorescent protein (GFP) reve

192 with the Discosoma red fluorescent protein (RFP) and orthotopically implanted onto the pancreas of n

193 PV(+) neurons with red fluorescent protein (RFP) and targeted them for cell-attached electrophysiolo

194 the Discomsoma sp. red fluorescent protein (RFP) and the enhanced green fluorescent protein (EGFP) r

195 in (GFP) and Myo3/5-red fluorescent protein (RFP) at nascent endocytic sites was revealed by two-colo

196 ha promoter and the red fluorescent protein (RFP) during lytic replication from the viral early PAN p

197 Indeed, red fluorescent protein (RFP) expression from mutated T-strand can be restored by

198 TH) promoter-driven red fluorescent protein (RFP) fluorescent reporter.

199 overy of functional red fluorescent protein (RFP) from DNA coding for a non-fluorescent variant of RF

200 luding an exon, and red fluorescent protein (RFP) from the mRNA lacking the exon; the other switches

201 ssion of ORF23 as a red fluorescent protein (RFP) fusion protein appeared to have a dominant negative

202 genetically fuse a red fluorescent protein (RFP) gene and two binding sites for an RNA-binding prote

203 T via expression of red fluorescent protein (RFP) in individual cells.

204 in the nucleus and red fluorescent protein (RFP) in the cytoplasm (HCT-116-GFP-RFP) were injected in

205 in the nucleus and red fluorescent protein (RFP) in the cytoplasm or with GFP only or RFP only were

206 in the nucleus and red fluorescent protein (RFP) in the cytoplasm.

207 leus and retroviral red fluorescent protein (RFP) in the cytoplasm.

208 was inserted into a red fluorescent protein (RFP) mCherry and a human Src tyrosine kinase to create i

209 porter mouse with a red fluorescent protein (RFP) sequence inserted into the interleukin-17F (IL-17F)

210 of exogenous Ealpha-red fluorescent protein (RFP) to the Ealpha-mCh yeast boosted the number of cytok

211 ) were labeled with red fluorescent protein (RFP) using retrograde viral infection.

212 onal RTE1 fusion to red fluorescent protein (RFP) was expressed under the control of the native RTE1

213 rafish in which the red fluorescent protein (RFP) was inserted into the pax8 gene.

214 .2 protein fused to red fluorescent protein (RFP) was localized to the plasma membrane and specifical

215 g a gene encoding a red fluorescent protein (RFP) whose expression correlates with the timing and lev

216 nt protein (GFP) or red fluorescent protein (RFP) with HA packaging regions (45 3' and 80 5' nucleoti

217 A GBP fusion to the red fluorescent protein (RFP), a molecule termed a chromobody, was previously use

218 of PV-Cre mice with red fluorescent protein (RFP), followed by targeted loose-patch recordings and tw

219 Red fluorescent protein (RFP)-expressing human cancer cell lines, including PC-3-

220 Using a red fluorescent protein (RFP)-expressing recombinant RRV (RRV-RFP), we show that

221 Stably transfected red fluorescent protein (RFP)-GRalpha NTM5 cell lines were developed.

222 ructures as well as red fluorescent protein (RFP)-labeled neurons within the mouse hippocampus are im

223 olocalizes with the red fluorescent protein (RFP)-labeled peroxisomal markers RFP-peroxisome targetin

224 olocalized with the red fluorescent protein (RFP)-tagged EV marker, CD63, after LPC treatment of cotr

225 at cells expressing red fluorescent protein (RFP)-tagged Htt protein containing 74 polyglutamine repe

226 pressing MIST1 with red fluorescent protein (RFP)-tagged pepsinogen C, a key secretory product of ZCs

227 ytes expressing NPY-red fluorescent protein (RFP).

228 AV vectors carrying red fluorescent protein (RFP).

229 ns, EB3, fused with red fluorescent protein (RFP).

230 nant RSV expressing red fluorescent protein (RFP).

231 , both small [i.e., red fluorescent protein (RFP)] and large (i.e., peroxisomes) substrates are effic

232 pH sensitivities of red fluorescent protein (RFP; pKa 4.5) and enhanced green fluorescent protein (EG

233 of spectrally distinct fluorescent proteins (RFP, YFP and CFP) in neighboring cells, creating a 'Brai

234 Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have

235 e advanced red-shifted fluorescent proteins (RFPs) has been developed.

236 ndicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visu

237 ion wavelengths of red fluorescent proteins (RFPs) make them attractive for whole-animal imaging beca

238 ing kinetics in 13 red fluorescent proteins (RFPs) with different chromophore environment and show th

239 ate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for

240 mly chosen cluster crossed over to providing RFP to eligible patients.

241 sistent with its attenuated activities, RecA-RFP nucleates onto double-stranded DNA approximately 3-f

242 As a consequence, RecA-RFP is proficient for DNA strand exchange with dATP or a

243 he wild-type protein, the activities of RecA-RFP are further enhanced by shifting the pH to 6.2.

244 mRFP1) to produce a functional protein (RecA-RFP) that is suitable for in vivo and in vitro analysis.

245 In vitro, the purified RecA-RFP protein forms a nucleoprotein filament whose k(cat)

246 rotein is also a dATPase; dATP supports RecA-RFP nucleoprotein filament formation in the presence of

247 In vivo, the RecA-RFP partially restores UV resistance, conjugational reco

248 Thus, RecA-RFP reveals that its attenuated biological functions cor

249 y, using single molecule visualization, RecA-RFP was seen to assemble into a continuous filament on d

250 nd that the GFPNESWdr68 fusion redistributed RFP-Dyrk1a to the cell cytoplasm potentially disconnecti

251 tein levels and accumulation of LC3 reporter RFP+ GFP+ (yellow) puncta, suggesting that HIV-1 infecti

252 f GFP-LdRab1:Q67L or GFP-LdRab1:S22N retains RFP-Ldgp63 in Golgi and blocks the secretion of Ldgp63,

253 t with tomato red fluorescent protein (rMERS-RFP) or deleted the entire ORF3, 4, and 5 accessory clus

254 rotein (RFP)-expressing recombinant RRV (RRV-RFP), we show that RRV particles are colocalized with ma

255 oci with fluorescent protein (FP) sequences (RFP and GFP respectively) by targeted integration via zi

256 In response to light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP

257 hortcoming, we developed a long Stokes shift RFP-based Ca(2+) indicator, REX-GECO1, with optimal two-

258 ells expressing red fluorescent protein (SL4-RFP) (1 x 10(6)) were injected into the spleen.

259 endosomes, because they colocalize with Snf7-RFP and because they correspond to a perivacuolar compar

260 Advantages and limitations of specific RFPs for each technique are presented.

261 Doppler echocardiography at rest and stress; RFP was defined as transmitral E:A ratio > or =1.0, isov

262 a single member of the p24 delta-1 subclass, RFP-p24delta5, which was dependent on the coiled-coil do

263 termined binding of GFP-tagged CaMKII to tag-RFP-labeled actin cytoskeleton within live cells using t

264 , lysosome function was normal, but a tandem RFP-GFP-LC3 failed to reach the lysosome even under cond

265 t protein (GFP) revealed that the N-terminal RFP domain migrated into the nucleus, while the C-termin

266 -clamp recordings of DA amacrine cells in TH-RFP mice and M1 ipRGCs in OPN4-EGFP mice.

267 brain dopaminergic neurons obtained from TH::RFP mice, immortalized DA neurons, and a heterologous sy

268 cal recordings of fluorescent neurons in TH::RFP (tyrosine hydroxylase gene promoter::red fluorescent

269 SC-CMs exhibited more mature phenotypes than RFP(-) cells in morphology, function and transcriptional

270 ses, and sequence analysis demonstrated that RFP was expressed from the appropriate consensus sequenc

271 ination of the axon morphology revealed that RFP(+) neurons include commissural bifurcating longitudi

272 The RFP intervention was associated with a 2-fold increased

273 When GFP spleen cells and the RFP cancer cells were coinjected in the PV, liver metast

274 Compared with standard care, the RFP group had a higher proportion of referrals due to fu

275 In contrast, the RFP(+) cells of pax2a/pax8 double mutants displayed alte

276 In pax8 homozygous fish, the RFP(+) cells underwent differentiation similar to that o

277 37 in the standard care group and 710 in the RFP group.

278 The luciferase insertion was made in the RFP-STAT3/STAT1-GFP cell line to have all three reporter

279 ing protein, whose translated product is the RFP protein alone.

280 ffects in the conformational dynamics of the RFP chromophore.

281 ortional to the associated expression of the RFP protein.

282 mphocytes were seen surrounding cells of the RFP tumor, which eventually regressed.

283 , the presence of 50 g/L lactose reduced the RFP intensity due to lactose-induced cytotoxicity.

284 e relationship between the properties of the RFPs of different phenotypes and their applications to v

285 Tumor RFP fluorescence facilitated real-time, sequential imagi

286 RNA oligonucleotides with partial wild-type RFP sequence, implying the involvement of plant DNA repa

287 lls expressing red fluorescent protein (U251-RFP) in zebrafish embryos.

288 After injection into the embryos, the U251-RFP cells proliferated and the resultant tumors, and eve

289 RFP-FimXDeltaREC, unlike RFP-FimX, is no longer localized to the bacterial pole,

290 Although several useful RFPs have been developed using directed evolution, the q

291 ith an endoplasmic reticulum marker, whereas RFP-tagged CHX17-CHX19 co-localized with prevacuolar com

292 To address this, we generated a fly in which RFP-Moesin and GFP-Moesin are expressed in mutually excl

293 ivo we find that EHBP-1-GFP colocalizes with RFP-RAB-10 on endosomal structures of the intestine and

294 by a previously undescribed interaction with RFP, which functions to recruit HDAC and/or Polycomb pro

295 vector is used for cytoplasmic labeling with RFP.

296 icol acetyltransferase, and thioredoxin with RFP, respectively, further substantiates the general app

297 NTM5 cells transfected with RFP-GRalpha showed a clear cytosolic localization of rec

298 7R was associated with worse outcome (5-year RFP, 76% for high v 86% for low expression; P = .001).

299 s a stronger predictor of recurrence (5-year RFP, 85% for high v 77% for low ratio; P = .004).

300 ) was associated with better outcome (5-year RFP, 90% for high v 80% for low expression; P = .026), w

301 ronments can be rationally modified to yield RFPs with novel photochemical properties.