ライフサイエンスコーパス: RNA cap

1 cursors to nucleoside tetraphosphate (Np(4)) RNA caps.

2 ose caps set them apart from other bacterial RNA caps.

3 t remove the y phosphates of ppp-RNAs during RNA capping.

4 TbCMT1 did not affect parasite growth or SL RNA capping.

5 at are essential to virus replication and to RNA capping.

6 gation but also in stimulating nascent viral RNA capping.

7 move the gamma phosphates of ppp-RNAs during RNA capping.

8 is is the first observation of ATP-dependent RNA capping.

9 the 5' RNA cap: G0pppAG-RNA --> (m7)G0pppAG-RNA ("cap-0")-->(m7)G0pppAm2'-O-G-RNA ("cap-1").

10 m7)G0pppAG-RNA ("cap-0")-->(m7)G0pppAm2'-O-G-RNA ("cap-1").

11 D6; mRNA capping enzyme subunits D1 and D12; RNA cap 2'-O-methyltransferase; A18 DNA helicase; DNA-de

12 y-mismatched receptor-binding o1 trimers and RNA-capping 2 pentamers balance competing needs of capsi

13 quent modifications characteristic of the SL RNA cap 4 were added successively in a 5' to 3' directio

14 le of this domain in the formation of the 5' RNA cap(6).

15 itute the N7- and 2'-O-methylated SARS-CoV-2 RNA cap ((7Me)GpppA(2'-O-Me)) using virally encoded non-

16 oded methyltransferases (MTases) involved in RNA capping, a guanine-N7-MTase and a ribose-2'-O-MTase.

17 e in complex with guanosine triphosphate and RNA cap analog.

18 establish NAD(+) as an alternative mammalian RNA cap and DXO as a deNADding enzyme modulating cellula

19 an alternatively be targeted, in addition to RNA cap and SAM pockets, for antiviral development.

20 Here, we introduce single cell RNA Cap And Tail sequencing (scRCAT-seq), a method to de

21 ein 5 (NS5) contains a methyltransferase for RNA capping and a polymerase for viral RNA synthesis.

22 replication protein 1a, which has N-terminal RNA capping and C-terminal helicase domains.

23 Here, we report a mechanism for SARS-CoV-2 RNA capping and document structural details at atomic re

24 transition from transcription initiation to RNA capping and elongation.

25 al surfaces of two of its proteins; that the RNA capping and export apparatus is a hollow cylinder, w

26 al protein 1 (nsP1) is responsible for viral RNA capping and gates the replication organelles by asse

27 ation factors 1a, with domains implicated in RNA capping and helicase functions, and 2a, with a centr

28 RNA replication proteins: 1a, which contains RNA capping and helicase-like domains, and 2a, which is

29 RNA replication proteins: 1a, which contains RNA capping and helicase-like domains, and the polymeras

30 1a, which encodes RNA capping and helicase-like domains, localizes to endo

31 esults extend our understanding of nidovirus RNA capping and methylation beyond coronaviruses and als

32 only improve the understanding of SARS-CoV-2 RNA capping and the mode of action of NAIs but also prov

33 will end with a summary of novel findings in RNA capping and the questions these findings pose.

34 l and functional understanding of alphavirus RNA-capping and the design of antivirals.

35 and an N-terminal domain implicated in viral RNA capping, and 2a contains a central polymerase-like d

36 icase-like domain and a domain implicated in RNA capping, and 2a, which contains a polymerase-like do

37 es incorporated into IAV mRNA, "snatched" 5' RNA caps, and corresponding RNA sequences from host RNAs

38 (ii) mutations D63A and Y248A, blocking the RNA capping; and (iii) mutation R252E, affecting nsP1 me

39 d that SARS-CoV-2 nsp12 is involved in viral RNA capping as a GTase, carrying out the addition of a G

40 Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that

41 Like the RNA-binding activity, RNA capping assays performed with open cores indicates t

42 our understanding of the mechanism of viral RNA cap binding we performed a detailed structural and b

43 dc42 effector, pp70 S6 kinase, stimulate the RNA cap-binding activity of the CBC.

44 Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important f

45 effects on pre-mRNA splicing is the nuclear RNA cap-binding complex (CBC), which plays a key role in

46 tion is followed by formation of a messenger RNA cap-binding complex that includes the initiation fac

47 protein 2 (ARS2), a component of the nuclear RNA CAP-binding complex that is crucial for biogenesis o

48 supported and the mechanism for GTP-mediated RNA capping by the SARS-CoV-2 NiRAN domain remains unres

49 Our data support the idea that coronavirus RNA capping could be targeted for development of antivir

50 h mTOR is known as a controller of messenger RNA cap-dependent translation initiation, new advances i

51 a multifunctional protein with an N-terminal RNA capping domain and a C-terminal helicase-like domain

52 on, RNA-dependent RNA polymerase (RdRp), and RNA capping domains.

53 The 1a protein has putative helicase and RNA-capping domains, whereas 2a contains a polymerase-li

54 RNA capping enzyme (CE) is recruited specifically to RNA

55 t of the RNA polymerase and also encodes the RNA capping enzyme guanylyltransferase.

56 -molecule inhibitors of the dengue virus NS5 RNA capping enzyme.

57 anylyltransferase activity of the flavivirus RNA capping enzyme.

58 We have examined the RNA-capping enzyme activities of bluetongue virus (BTV)

59 its well-characterized function as the viral RNA-capping enzyme.IMPORTANCE Rotaviruses are significan

60 Recent structural studies have shown that RNA capping enzymes and DNA ligases have similar protein

61 (+)-dependent DNA ligases, and GTP-dependent RNA capping enzymes are members of a covalent nucleotidy

62 t contemporary DNA ligases, RNA ligases, and RNA capping enzymes evolved by the fusion of ancillary e

63 Novel applications of RNA capping enzymes in the discovery of new RNA species

64 For example, RNA capping enzymes possess triphosphatase domains that

65 For example, RNA capping enzymes possess triphosphatase domains that

66 at comprise the active sites of DNA ligases, RNA capping enzymes, and T4 RNA ligase 2.

67 at comprise the active sites of DNA ligases, RNA capping enzymes, and T4 RNA ligases 1 and 2.

68 f a shared structural basis for catalysis by RNA capping enzymes, DNA ligases, and RNA ligases, which

69 superfamily of RNA ligases, DNA ligases, and RNA capping enzymes.

70 anism and origin of RNA and DNA ligases, and RNA capping enzymes.

71 and V that are conserved in DNA ligases and RNA capping enzymes.

72 ped' by the addition of GMP to the 5" end by RNA capping enzymes.

73 polynucleotide ligases and the GTP-dependent RNA capping enzymes.

74 polynucleotide ligases and the GTP-dependent RNA capping enzymes.

75 xtend our current understanding of nidovirus RNA cap formation and methylation beyond the coronavirus

76 e, and the recently recognized Mesoniviridae RNA cap formation and methylation have been best studied

77 ping enzyme guanylyltransferase activity and RNA cap formation by transcription factor IIH-mediated C

78 sferase activity of human capping enzyme and RNA cap formation.

79 protein catalyzes two reactions involved in RNA cap formation.

80 ect evidence for the importance of the viral RNA capping function.

81 protein contains a helicase-like domain and RNA capping functions.

82 es, responsible for replication fidelity and RNA cap G-N-7 methylation, respectively.

83 pAG-RNA), leading to the formation of the 5' RNA cap: G0pppAG-RNA --> (m7)G0pppAG-RNA ("cap-0")-->(m7

84 lates the N7 and 2'-O positions of the viral RNA cap (GpppA-RNA --> m(7)GpppA-RNA --> m(7)GpppAm-RNA)

85 7 and ribose 2'-OH methylations of the viral RNA cap (GpppA-RNA-->m(7)GpppAm-RNA).

86 ally catalyzes two methylations of the viral RNA cap, GpppA-RNA-->m(7)GpppA-RNA-->m(7)GpppAm-RNA, by

87 de for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' e

88 RNA cap guanine-N2 methyltransferases such as Schizosacc

89 In humans, two methyltransferases, RNA cap guanine-N7 methyltransferase (hRNMT) and cap-spe

90 The smaller size of RNA capping guanylyltransferases from other organisms su

91 The occurrence of NAD(+) as a non-canonical RNA cap has been demonstrated in diverse organisms.

92 NAD(+) was recently reported to serve as an RNA cap in both eukaryotes and prokaryotes.

93 ifampicin-binding pocket, suggesting altered RNA capping in Rifampicin-resistant strains.

94 nd Caenorhabditis elegans we have found that RNA capping is also essential for metazoan viability.

95 systems level, CapQuant can reveal both the RNA cap landscape and the transcription start site distr

96 the mRNA capping apparatus of VSV evolved an RNA capping machinery that functions in a sequence-speci

97 strikingly diverged positions of N-proximal RNA capping/membrane binding domains.

98 ture reveals many mechanistic details of the RNA capping/membrane binding domains.

99 Thus, RNA cap methylation is an attractive target for antivira

100 f SARS-CoV-2 nsp14 protein involved in viral RNA cap modification.

101 GFP) showed that sequences in the N-terminal RNA capping module of 1a mediate membrane association.

102 typically contains the adenosine 2'OH of the RNA-cap moiety.

103 ose a wide repertoire of potential bacterial RNA capping molecules, and provide mechanistic insights

104 nal enzyme bearing 3'-5' exoribonuclease and RNA cap N7-guanine methyltransferase activities involved

105 Enzymes involved in RNA capping of SARS-CoV-2 are essential for the stabilit

106 ty of accurately and sensitively quantifying RNA caps on a systems level, CapQuant can reveal both th

107 hosphatase (5'RTP), which is involved in the RNA capping process.

108 iphosphates and thiotriphosphates (including RNA cap reagents).

109 erases; however, the mechanism of SARS-CoV-2 RNA capping remains poorly understood.

110 ties involved in replication fidelity and 5'-RNA capping, respectively.

111 e central pore of the membrane-anchored nsP1 RNA-capping ring.

112 By establishing a small RNA Cap-seq method that employs the cap-binding protein

113 tivity which catalyses the first step in the RNA-capping sequence.

114 ty and amino acid requirements typical of an RNA cap-specific, m(7)G-dependent N2 methyltransferase.

115 g with cellular metabolites as a novel viral RNA-capping strategy, which could be used by other virus

116 These results define an additional 5' RNA cap structure in eukaryotes and raise the possibilit

117 The SL RNA cap structure in Trypanosoma brucei is unique among

118 The only known RNA cap structure in unicellular protists is the unusual

119 For coronaviruses, RNA cap structure is first methylated at the guanine-N-7

120 also utilize GTP to produce an authentic 5' RNA cap structure, though the GTP-mediated mechanism is

121 (N1 2'-O-Me), creating part of the mammalian RNA cap structure.

122 SARS-CoV-2 catalyzes the formation of the 5' RNA cap structure.

123 e, which are involved in the modification of RNA cap structure.

124 yme is responsible for methylating the viral RNA cap structure.

125 el of how the flavivirus MTase protein binds RNA cap structures is presented.

126 NSP14 plays a critical role in viral RNA cap synthesis and its inhibition represents a novel

127 s to both N7 and 2'-O positions of the viral RNA cap, the GTP-binding pocket functions only during th

128 Tgs1 is the enzyme that converts m(7)G RNA caps to the 2,2,7-trimethylguanosine (TMG) caps char

129 responsible for converting 7-methylguanosine RNA caps to the 2,2,7-trimethylguanosine cap structures

130 s initially identified as being required for RNA cap trimethylation in vivo in budding yeast.

131 erminal domain in the iris of the pentameric RNA-capping turret.

132 and the nsp16/nsp10 complex completes viral RNA capping via its 2'-O-methyltransferase.

133 ific binding of the methyltransferase to the RNA cap was demonstrated by UV cross-linking to [32P]GMP

134 To facilitate analysis of RNA caps, we developed a systems-level mass spectrometry

135 The RNA capped with the m(7(LNA))G[5']ppp[5']G 3 cap analogu