ライフサイエンスコーパス: RNA probe

1 chemistry using a 35S-labelled complementary RNA probe.

2 ridization using a CYP2J2-specific antisense RNA probe.

3 t DNA to liberate and hybridize with another RNA probe.

4 ZFP36L1 mutant, bound to the Fgf21 3'UTR ARE RNA probe.

5 ry using an intron-specific VP heteronuclear RNA probe.

6 und to bind strongly and specifically to the RNA probe.

7 lity of the fusion protein to stably bind an RNA probe.

8 e by in situ hybridization using 35S-labeled RNA probes.

9 ine pyrene as a fluorescent label for DNA or RNA probes.

10 75 mRNA using digoxigenin-labeled anti-sense RNA probes.

11 tive miRNA detection is achieved using 2'OMe-RNA probes.

12 tinas after labeling with cell type-specific RNA probes.

13 situ hybridization with digoxigenin-labeled RNA probes.

14 d it does not bind to single-stranded DNA or RNA probes.

15 sections of chick scleras using 33P-labeled RNA probes.

16 eq for transcriptome-wide structure-specific RNA probing.

17 In situ hybridization with an RNA probe against vascular endothelial growth factor rec

18 eptible to RNAse H enzymatic cleavage of the RNA probe, allowing the target DNA to liberate and hybri

19 RNA probing analyses outlined a specific UTR1 conformati

20 hybridization using a specific 16S ribosomal RNA probe and genomic DNA probe.

21 ation assay using chemiluminescence with HPV RNA probes and by polymerase chain reaction using SPF10

22 uorescently labeled mRNA-specific anti-sense RNA probes and dsRNA-binding protein to identify the exp

23 -linking with synthetic diazirine-containing RNA probes and quantitative proteomics to profile RNA-pr

24 ection of five differently labeled antisense RNA probes and up to seven differ-ent transcripts in a s

25 Using RNA probing and mutagenesis, we demonstrate that the HIV

26 ic activity both in vitro, using a synthetic RNA probe, and in vivo, by quantifying endogenous levels

27 luorescein-urease conjugate with the labeled RNA probe, and quantitative detection of the membrane-bo

28 pecific sequences, pathogen-specific DNA and RNA probes, and identification of pathogen-specific ions

29 We used messenger RNA probes, antibodies, a LacZ reporter mouse model, and

30 Next, the short DNA sequence and the RNA probe are linked using a T4 DNA ligase.

31 This continuously happens until all of the RNA probes are cleaved, leaving the nanoparticles unprot

32 ular genetic strategy in which two different RNA probes are hybridized to adjacent positions on a tar

33 RNA probes are hybridized to fixed, mixed-staged Drosoph

34 DNA and RNA probes are important analytical reagents in molecula

35 Digoxygenin (dig)-labeled antisense RNA probes are in vitro transcribed from a template synt

36 ) and light (NF-L) NF mRNAs when radioactive RNA probes are incubated with high-speed supernatants (S

37 Aptamers, single-stranded DNA/RNA probes, are poised to become a chemist's antibody an

38 TP potentiated TTP binding to ARE-containing RNA probes, as determined by RNA gel shift assays; AUF1p

39 lished with the target miRNA for a thiolated RNA probe assembled onto a gold nanoparticles (AuNPs) mo

40 itu hybridization, by employing an antisense RNA probe; BDNF protein was detected by employing a sens

41 bes are an attractive alternative to DNA and RNA probes because they are chemically and biologically

42 mic (MD) simulations recently suggested that RNA-probe binding can be cooperative, leading to measure

43 onucleotides are incorporated into antisense RNA probes by in vitro transcription.

44 g the nCounter system and 1,412 genes (4,998 RNA probes) by using next-generation sequencing (NGS).

45 hybridization of the cDNA PCR product to an RNA probe, capture of the RNA-DNA hybrid on a solid phas

46 RNA probes carrying the sequence GCGC in place of the ce

47 yses of datasets generated with a variety of RNA probing chemistries demonstrate the value of diffBUM

48 nd monkeys, using in situ hybridization with RNA probes complementary to Cad6, Dlx1, Dlx2, Dlx5, Gbx2

49 With this approach, RNA probes complementary to mRNA targets trigger chain r

50 A Northern blot assay using single-stranded RNA probes complementary to the spliced in vivo 8.4-kb l

51 Proteins in cell extracts bind to an RNA probe containing six copies of the cycling sequence.

52 A Gtx RNA probe containing this complementarity could be photo

53 e screened a cDNA expression library with an RNA probe containing this sequence.

54 As high-quality RNA probing data become widely available, structurally-i

55 l-principled framework, which in turn allows RNA probing data to be easily integrated into a wide ran

56 Biotinylated RNA probes deleted of the conserved CAGA motif in the te

57 RNA probing demonstrated that the drug protects a specif

58 The 526 base pair antisense, but not sense, RNA probe derived from exons 10-13 stained cystic fibros

59 tic mobility-shift assays using radiolabeled RNA probes derived from different regions of cyclin D1 m

60 In situ hybridization using RNA probes derived from this clone revealed that alveola

61 7 allergen coding sequence with appropriate RNA probes designed and described for the first time in

62 method takes advantage of inexpensive, long RNA probes detected with antibodies, and we present nove

63 RNA probes encoding the APP IRE stem loop exhibited the

64 obility gel shift analyses using a series of RNA probes encompassing the entire GROalpha transcript.

65 e reaction via the formation of single probe-RNA-probe (enzyme) complex on magnetic beads.

66 RNA probing experiments demonstrated that the fourU elem

67 lection from the lysate using a biotinylated-RNA probe followed by mass spectrometry identified methy

68 We used the cDNA to make an RNA probe for a ribonuclease protection assay (RPA).

69 We then used the RNA probe for in situ hybridization (ISH) experiments.

70 and nucleoprotein, double stranded RNA, and RNA probe for spike genes were evaluated for the ability

71 Subsequently, a biotinylated RNA probe for the HIC was transcribed from a nested sequ

72 A similar fraction hybridize with RNA probes for GAD65 and GAD67.

73 was cloned and used to synthesize antisense RNA probes for in situ hybridization analyses of zebrafi

74 We have developed RNA probes for the direct identification of wild poliovi

75 Using RNA probes from SR30 intron 10, whose splicing is altere

76 RNA probes from wild-type and Brn3b(-/-) E14.5, E16.5 an

77 olarization assay that works well, even with RNA probes >90 nucleotides long.

78 Also, RNA probe hybridization confirmed their belonging to the

79 Single-stranded U16 and U17 gene-specific RNA probes hybridized with at least five RNA species fro

80 his system utilizes capture of the protected RNA probe hybrids to streptavidin-coated membranes attac

81 esults from Northwestern analysis using a PU-RNA probe identified the regions within Puralpha that ar

82 ic protein to more than 240 000 unstructured RNA probes in one experiment.

83 ric reorganization of protein, ribosome, and RNA probes in the cytoplasm.

84 situ hybridization with digoxigenin-labeled RNA probes in tissue sections of human jejunum.

85 situ hybridization using digoxigenin-labeled RNA probes in wholemounts and tissue sections.

86 iling of up to 44 proteins and 96 genes (928 RNA probes) in lymphoid, colorectal tumor and autoimmune

87 he electroporation-based delivery of DNA and RNA probes into mammalian cells.

88 e used antisense, sense, and double-stranded RNA probes made from the Marsilea centrin cDNA, MvCen1,

89 1 open cores were incubated with 32P-labeled RNA probes of viral and nonviral origin and the reaction

90 ve antigens for protein detection and enable RNA probe penetration for IF/FISH, we perform IF before

91 Unlike conventional sequence-specific DNA or RNA probes, polyamides can recognize their target sequen

92 With the use of RNA probes progressively upstream of the translational s

93 By RNA probe protection we found that transcripts of SpRunt

94 f each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay.

95 In situ hybridization using a radioactive RNA probe resulted in distinct signals in the developing

96 orthern analysis using a VMAT2 complementary RNA probe revealed a single 4 kb mRNA species in corpus

97 In situ hybridization with an RNA probe revealed that the gene is widely expressed; ho

98 ort, biotinylated DNA oligonucleotide and an RNA probe sequence to DNA templates spotted onto a maste

99 er average in a moving window around a given RNA probe set, biologically relevant information can be

100 tu hybridization using a digoxigenin-labeled RNA probe showed that the tissue distribution of amelobl

101 pen) virion-derived cores and virus-specific RNA probes showed that VP3 has affinity for single-stran

102 studies using EBV Epstein-Barr viral-encoded RNA probes showed viral RNA expression in both CD8(+) T-

103 platform involves immobilization of a 40-mer RNA probe specific for a characteristic fragment of the

104 o assess PIC gene expression using antisense RNA probes specific for bovine interleukin-1alpha (IL-1a

105 Finally, using RNA probes specific for RSV genomic RNA, we found that v

106 mRNAs in the adult gut with single-stranded RNA probes specific for trkA, trkB, and trkC.

107 We designed radiolabeled antisense RNA probes, specific for the "adult" Na(V)1.4 and "fetal

108 ER-Library; and a DNA capture approach using RNA probes targeting both DNA strands, termed DEEPER-Cap

109 DNA Input with Probe-based DNA Enrichment by RNA probes targeting DNA duplex (DEEPER-Seq).

110 ge in the dynamics of single-stranded DNA or RNA probes tethered to an Au electrode on immunospecific

111 tracts bound less well to an AU-rich element RNA probe than did the same amount of TTP following deph

112 exhibit a similar increase in binding to the RNA probe that contains the direct repeat of the pH-RE.

113 protection assays using a 710 bp anti-sense RNA probe that spanned the alternatively spliced and nat

114 sults were obtained with a small interfering RNA probe that specifically inhibited PP2A expression.

115 d a nonisotopic RNase protection assay using RNA probes that are dual-labeled with biotin and fluores

116 The assay uses RNA probes that hybridize to ACT1 intron RNA.

117 sing digoxigenin-labeled sense and antisense RNA probes that recognize chicken Pax-6 and Prox 1 (whos

118 sample sequences are hybridized to the same RNA probe, the hybrids are partially digested with RNAse

119 attached to affinity reagents (antibodies or RNA probes) through a photocleavable (PC) linker; and (3

120 RNA probe titrations and RT-PCR demonstrated that BRCA1

121 we have used an antibody and a complementary RNA probe to explore the distribution of neurons that ex

122 In situ hybridization, using RNA probes to 20 of these sequences, demonstrates differ

123 was directly correlated with the ability of RNA probes to bind to ribosomes.

124 Furthermore, we use RNA probes to show that selective inhibition of E2F3, no

125 scribes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos.

126 s based on hybridization of complex (cDNA or RNA) probes to cDNA microarrays, either on glass slides

127 el shift assays; AUF1p45 did not bind to the RNA probes under these conditions.

128 Percent relative cleavage of RNA probes was recorded at multiple time points from tri

129 zation (ISH) using digoxigenin (DIG)-labeled RNA probes was used to localize mRNA for IL-6 and TNF al

130 Using a single-strand RNA probe, we demonstrated that FSHR mRNA expression is

131 protein has no detectable activity toward an RNA probe, we engineered an RNA-reactive variant, called

132 In hindbrain, AptCB1R RNA probe weakly labeled inhibitory interneurons in the

133 In diencephalon, AptCB1R RNA probe weakly stained the central-posterior (CP) and

134 ellular localization, Y2 sense and antisense RNA probes were hybridized to female mouse lacrimal glan

135 Biotinylated RNA probes were used to isolate additional proteins that

136 a template for the synthesis of an antisense RNA probe, which is labeled with digoxigenin-linked nucl

137 sizes, on Northern blots of Caco-2 poly(A)+ RNA probed with a 630 bp 5' hPepT1 cDNA probe, correspon

138 We hybridized ovule RNA probes with Affymetrix ATH1 genome arrays and valida

139 nsfer interactions yield fluorescent DNA and RNA probes with dual emission color readout.

140 RNA probes with perfect complementarity to 18S or 28S rR