ライフサイエンスコーパス: RNase III

1 RNase III catalyzed hydrolysis is slower at low pH, perm

2 RNase III cleaves a hairpin structure within the arfA-co

3 RNase III enzyme Drosha interacts with DGCR8 to form the

4 RNase III family enzymes, which are perhaps the most wid

5 RNase III family members also exhibit a "catalytic" doma

6 RNase III is a global regulator of gene expression in Es

7 RNase III is a key enzyme in the pathways of RNA degrada

8 RNase III is subject to multiple levels of control, refl

9 RNase III proteins recognize double-stranded RNA structu

10 RNase III recognizes its substrates and selects the scis

11 RNase III-deficient cells are hypersensitive to high iro

12 RNase III[D45A], RNase III[D45E], and RNase III[D45N] ex

13 RNase III[D45E] activity is partially rescued by Mn(2+).

14 RNase III[E38A], RNase III[E65A], and RNase III[E100A] a

15 here an RNA, derived from the T7 phage R1.1 RNase III substrate, that is resistant to cleavage in vi

16 In the presence of Mg(2+) RNase III[DeltadsRBD] is less efficient than the wild-ty

17 pecific knockout of Dicer, a gene encoding a RNase III endonuclease essential for microRNA (miRNA) pr

18 e biochemical properties of A. aeolicus (Aa)-RNase III and the reactivity epitopes of its substrates

19 Aa-RNase III cleavage of the pre-16S substrate is blocked b

20 atalytic activity of purified recombinant Aa-RNase III exhibits a temperature optimum of approximatel

21 that a conserved glutamine (Q157) in the Aa-RNase III dsRNA-binding domain (dsRBD) directly interact

22 tion-related mRNAs by the S. coelicolor AbsB/RNase III enzyme occurs largely by ribonucleolytic cleav

23 e of the nuclease domain of Aquifex aeolicus RNase III, the E41, D114, and E117 side chains of E. col

24 transcriptomes of S. coelicolor M145 and an RNase III (rnc)-null mutant of that strain.

25 g Dicers, QDE-2, the exonuclease QIP, and an RNase III domain-containing protein, MRPL3.

26 rough directed cleavage of transcripts by an RNase III enzyme, Rnt1p.

27 ed in S. antibioticus may be processed by an RNase III-like activity, transcripts originating from Pp

28 talyzes editing in T. brucei and contains an RNase III motif that suggests nuclease function.

29 For this study, we created an RNase III null mutant of Streptococcus pyogenes and its

30 Dicer, an RNase III endonuclease, is an essential component of the

31 amining the effect of knocking out Dicer, an RNase III enzyme required for miRNA and small interferin

32 Using conditional knock-out of Dicer, an RNase III enzyme required for miRNA maturation, previous

33 though it is clear that miRNAs and Dicer, an RNase III enzyme that is central to the production of ma

34 Dicer, an RNase III enzyme, initiates RNA interference by processi

35 NAs) in vivo through the action of Dicer, an RNase III-family enzyme.

36 Loss of Dicer, an RNase III-like enzyme critical in microRNA biogenesis, c

37 In support of this, Dicer, an RNase III-like enzyme that controls the maturation of mi

38 DICER1, an RNase III endonuclease encoded by Dicer1, is required fo

39 Microprocessor contains Drosha, an RNase III endonuclease, and DGCR8, a gene deleted in DiG

40 Streptomyces coelicolor absB gene encodes an RNase III family endoribonuclease and is normally essent

41 Primer extension with RNA from an RNase III null mutant of Streptomyces coelicolor M145 an

42 at editosomes with KREPB2, which also has an RNase III motif, specifically cleave cytochrome oxidase

43 the expression of the rpsO-pnp operon in an RNase III (rnc) mutant of Streptomyces coelicolor.

44 and RpoS protein levels are increased in an RNase III mutant strain with or without the sRNAs, sugge

45 hat PNPase expression is autoregulated in an RNase III-dependent manner in S. coelicolor.

46 Dicer is an RNase III endonuclease which processes miRNA precursors

47 DICER1 is an RNase III endoribonuclease central to miRNA biogenesis,

48 Dicer is an RNase III enzyme essential for the maturation of the maj

49 Drosha is an RNase III enzyme that was recently implicated in miRNA p

50 Dicer is an RNase III-family nuclease that initiates RNA interferenc

51 Here, we present the crystal structure of an RNase III-product complex, the first catalytic complex o

52 Rnc70, an RNase III mutant that binds but does not cleave rIII, al

53 A contains, in this order: the NUTL site, an RNase III-sensitive hairpin and the N ribosome-binding s

54 RNase III[D45A], RNase III[D45E], and RNase III[D45N] exhibit negligible activities, regardles

55 While RNase III[E41A] and RNase III[D114A] have K(Mg) values that are approximatel

56 t is shown here that the RNase III[E41A] and RNase III[D114A] mutants exhibit catalytic activities in

57 RNase III[E38A], RNase III[E65A], and RNase III[E100A] also require higher Mg(2+) concentratio

58 suring co-ordination of 16S rRNA folding and RNase III processing that results in production of prope

59 egulated by transcriptional interference and RNase III processing at the overlapping region.

60 The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base

61 In fact, by preventing N autoregulation, RNase III activates N gene translation at least 200-fold

62 Bacterial RNase III orthologs cleave their substrates in a highly

63 Bacterial RNase III orthologs exhibit the simplest structures, wit

64 n on a post-catalytic complex of a bacterial RNase III bound to the cleaved minimal substrate.

65 (RNase III) family represented by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer.

66 t role in substrate recognition by bacterial RNase III.

67 -nt siRNA-like species produced by bacterial RNase III.

68 gous RNase III domains from either bacterial RNase III enzymes or Giardia Dicer.

69 led a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, an

70 tive determinants of reactivity of bacterial RNase III substrates.

71 es coelicolor encodes a homolog of bacterial RNase III.

72 interest, the structurally simpler bacterial RNase III serves as a paradigm for the entire family.

73 For mechanistic studies, bacterial RNase III has been a valuable model system for the famil

74 he Tetrahymena genome, two that contain both RNase III and RNA helicase motifs, Dicer 1 (DCR1) and DC

75 Bs-RNase III was found to cleave precursor scRNA at two sit

76 t of the RNase III-like endoribonuclease, Bs-RNase III, in scRNA processing.

77 Neither Bs-RNase III nor RNase M5, the two known narrow-specificity

78 Here we show that Bs-RNase III cleaves primarily at the 5' cleavage site and

79 no acids that are essential for catalysis by RNase III and Dicer are missing from the RNase III domai

80 stand substrate recognition and catalysis by RNase III, we examined steady-state and pre-steady-state

81 tudies on the mechanism of dsRNA cleavage by RNase III have focused mainly on the enzymes from mesoph

82 and Pasteurella multocida are all cleaved by RNase III as predicted, whereas the hairpin from Neisser

83 tains a hairpin structure that is cleaved by RNase III to produce a non-stop transcript.

84 ii and Salmonella typhimurium are cleaved by RNase III when expressed in E. coli.

85 dsRNA elimination by RNase III treatment prior to DRIPc-seq allowed the genom

86 ssed from viral replication intermediates by RNase III-like enzyme Dicer guide sequence-specific anti

87 dy, we show that cleavage of the N leader by RNase III does not inhibit antitermination but prevents

88 dation by an additional pathway, mediated by RNase III, which, in contrast to the RNase E-mediated pa

89 involves cleavage of structured precursor by RNase III-like endonucleases.

90 R1.1[CL3B] RNA is recognized by RNase III in the same manner as R1.1 RNA, as revealed by

91 es from other bacteria are also regulated by RNase III and tmRNA.

92 ADI1 is controlled post-transcriptionally by RNase III cleavage.

93 as a major antideterminant in S. cerevisiae RNase III activity, and suggest a rationale for their ap

94 nditions in the absence of the S. cerevisiae RNase III ortholog Rnt1p or of the nuclear exosome compo

95 he structure of the Saccharomyces cerevisiae RNase III (Rnt1p) postcleavage complex and explain why R

96 t1p, the only known Saccharomyces cerevisiae RNase III double-stranded RNA endonuclease, plays import

97 The Saccharomyces cerevisiae RNase III enzyme Rnt1p preferentially binds to double-st

98 e in assays employing purified S. coelicolor RNase III.

99 e E41, D114, and E117 side chains of E. coli RNase III are expected to be coordinated to a divalent m

100 Some E. coli RNase III substrates contain an internal loop, in which

101 E. coli RNase III-dependent cleavage events can regulate gene ex

102 is resistant to cleavage in vitro by E.coli RNase III but retains comparable binding affinity.

103 It has been proposed that E.coli RNase III can function in a non-catalytic manner, by bin

104 ns in their catalytic sites--inhibits E.coli RNase III cleavage of R1.1 RNA.

105 E.coli RNase III is a member of a structurally distinct superfa

106 E.coli RNase III requires a divalent metal ion for activity, wi

107 A minimal substrate of E.coli RNase III, muR1.1 RNA, was characterized and used to def

108 cidic amino acids, which in Escherichia coli RNase III are E38, E41, D45, E65, E100, D114, and E117.

109 ws that a truncated form of Escherichia coli RNase III lacking the dsRBD (RNase III[DeltadsRBD]) can

110 Escherichia coli RNase III participates in the maturation of the ribosoma

111 processing reactivities of Escherichia coli RNase III substrates are determined in part by the seque

112 Here we report the use of Escherichia coli RNase III to cleave double-stranded RNA (dsRNA) into end

113 munoprecipitated dsRNA from Escherichia coli RNase III WT and mutant strains were deep-sequenced.

114 he generation and use of an Escherichia coli RNase III-prepared human kinesin/dynein esiRNA library t

115 NMD can also complement RNase III-mediated nuclear degradation of unspliced RPS2

116 DICER is a cytoplasmic RNase III enzyme that not only cleaves precursor miRNAs

117 The next cleavage employs the cytoplasmic RNase III Dicer producing miRNA duplexes [3, 4].

118 RNase III[D45A], RNase III[D45E], and RNase III[D45N] exhibit negligible

119 Hence, the degenerate RNase III domain and a newly identified domain are criti

120 precursor substrates by the Drosha and Dicer RNase III enzymes.

121 istinct ~20S editosomes contains a different RNase III-type endonuclease, 1 of 3 related proteins and

122 tional processing of transcripts by directed RNase III (Rnt1p) cleavage, were shown to provide predic

123 Drosha RNase III catalyzes the first excision event, the releas

124 scherichia coli RNase III lacking the dsRBD (RNase III[DeltadsRBD]) can accurately cleave small proce

125 RNase III[E38A], RNase III[E65A], and RNase III[E100A] also require highe

126 Escherichia coli (Ec) RNase III catalytic activity is known to increase during

127 previously shown that Escherichia coli (Ec) RNase III recognizes dsRNA with little sequence specific

128 nition determinant for Escherichia coli (Ec) RNase III substrates.

129 In fact, Tm-RNase III cleaves an Ec-RNase III substrate with identical specificity and is in

130 d by antideterminant bp that also inhibit Ec-RNase III.

131 This study shows that Ec-RNase III is phosphorylated on serine in vitro by purifi

132 sequence is similar to that observed with Ec-RNase III substrates.

133 export membrane protein), and rnc (encoding RNase III) genes in a secF-nuoF-lepB-rnc cluster.

134 interactions between ybeY and rnc (encoding RNase III), ybeY and rnr (encoding RNase R), and ybeY an

135 double-strand RNA-specific endoribonuclease RNase III.

136 that the narrow-specificity endoribonuclease RNase III and the 5' exonuclease RNase J1 are not essent

137 The endoribonuclease RNase III cleaves double stranded RNAs, which can be for

138 oscopy and a human kinesin endoribonucleases RNase III-prepared short interfering RNA (esiRNA) librar

139 e deleted Dicer1, which encodes an essential RNase III enzyme for miRNA biogenesis, in murine CCAAT/e

140 tic residues conserved throughout eukaryotic RNase III enzymes.

141 been well characterized, but how eukaryotic RNase IIIs work is less clear.

142 ide a framework for understanding eukaryotic RNase IIIs.

143 eins, but are typified by mutually exclusive RNase III endonucleases with distinct cleavage specifici

144 her the catalytic properties of extremophile RNases III nor the structures and reactivities of their

145 ative constraints that would incur following RNase III processing of a genomic hairpin.

146 able to repress zorO in a strain deleted for RNase III, indicating that repression requires cleavage

147 the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction.

148 ation results to identify new substrates for RNase III cleavage.

149 -type E. coli and from strains defective for RNases III and E, two RNases reported to be involved in

150 Indeed CaDcr1, the sole functional RNase III enzyme in C. albicans, has additional function

151 The sequences of 79 fungal RNase III substrates were inspected to identify addition

152 s were used to identify potential new genes, RNase III cleavage sites and the direct or indirect cont

153 previously reported structures of homologous RNase III domains from either bacterial RNase III enzyme

154 We examine here how RNase III itself is regulated in response to growth and

155 ted from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface

156 protein that contains two ribonuclease III (RNase III) domains, the domain that harbors the active s

157 e such protein family, the ribonuclease III (RNase III) endonucleases, includes Rnt1, which functions

158 Ribonuclease III (RNase III) enzymes are a family of double-stranded RNA (

159 The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsR

160 Members of the ribonuclease III (RNase III) family are double-stranded RNA (dsRNA) specif

161 produced by members of the ribonuclease III (RNase III) family represented by bacterial RNase III and

162 Ribonuclease III (RNase III) is a conserved, gene-regulatory bacterial end

163 cessing are members of the ribonuclease III (RNase III) superfamily, which are highly conserved in eu

164 Escherichia coli ribonuclease III (RNase III; EC 3.1.24) is a double-stranded(ds)-RNA-speci

165 These data implicate RNase III recognition of viral RNA as an antiviral defen

166 tions, defined by four RNA binding motifs in RNase III and three protein-interacting boxes in dsRNA,

167 at a specific position in the pb to inhibit RNase III binding is due to the purine 2-amino group, wh

168 recognition antideterminants, which inhibit RNase III binding.

169 g double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes.

170 Although crRNA 3' end formation involves RNase III and trans-encoded tracrRNA, as in other type I

171 Editosomes with the KREN1 or KREN2 RNase III type endonucleases specifically cleave deletio

172 dthrough transcript requires mutants lacking RNase III, we detect readthrough transcripts in wild-typ

173 4, two Arabidopsis thaliana chloroplast Mini-RNase III-like enzymes sharing 75% amino acid sequence i

174 -strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively.

175 A model is proposed whereby one or more RNase III-type endonucleases mediate the initial binding

176 In Drosophila melanogaster, the multidomain RNase III Dicer-1 (Dcr-1) functions in tandem with the d

177 mRNA preparation from JSE1880 using a mutant RNase III protein that binds to transcripts but does not

178 ei which contains a degenerate, noncatalytic RNase III domain.

179 DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microR

180 icroprocessor, which consists of the nuclear RNase III Drosha and the double-stranded RNA-binding dom

181 The nuclear RNase III Drosha catalyzes the first processing step tog

182 at1p, the nuclear exosome and by the nuclear RNase III endonuclease Rnt1p to prevent undesired expres

183 ear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pr

184 ge of the primary transcripts by the nuclear RNase III enzyme Drosha.

185 ng sequences were affected in the absence of RNase III.

186 The broad cellular actions of RNase III family enzymes include ribosomal RNA (rRNA) pr

187 Optimal activity of RNase III[DeltadsRBD] is observed at low salt concentrat

188 The biochemical behavior of RNase III of the hyperthermophilic bacterium Thermotoga

189 is mediated by Microprocessor, comprised of RNase III enzyme Drosha and its cofactor DGCR8.

190 oth phenotypes are suppressed by deletion of RNase III.

191 tes that the db is a positive determinant of RNase III recognition, with the canonical UA/UG sequence

192 e C-terminal dsRNA-binding domain (dsRBD) of RNase III, indicating that EB perturbs substrate recogni

193 s, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (d

194 process requiring RNase E but independent of RNase III, the RNA chaperone Hfq, and the regulatory pep

195 cal role in lambda development, the level of RNase III activity therefore serves as an important sens

196 of these findings are discussed in light of RNase III substrate function as a gene regulatory elemen

197 roximately 2.8-fold larger than the K(Mg) of RNase III (0.46 mM), the RNase III[E41A/D114A] double mu

198 are cleaved by Rnt1p, the yeast ortholog of RNase III, which creates an entry site for complete degr

199 ed in the absence of the yeast orthologue of RNase III Rnt1p or of the 5' --> 3' exonucleases Xrn1p a

200 nce between nutL and nutR is the presence of RNase III processing sites (rIII) located immediately pr

201 We discovered that down-regulation of RNase III activity occurs during both stresses and is de

202 clease-binding proteins in the regulation of RNase III activity.

203 te, reflecting the growth rate regulation of RNase III expression itself.

204 eviously unknown function, is a regulator of RNase III cleavages.

205 thout the sRNAs, suggesting that the role of RNase III in this context is to reduce the translation o

206 , the domain that harbors the active site of RNase III and Dicer enzymes.

207 e presence of hitherto-undiscovered sites of RNase III cleavage of the pnp transcript.

208 Here, we present three crystal structures of RNase III in complex with double-stranded RNA, demonstra

209 rocessing, gained from structural studies of RNase III, is reviewed.

210 trans-acting sRNAs that can be substrates of RNase III.

211 nic regions appeared to be direct targets of RNase III.

212 pre-rRNA processing is identical to that of RNase III in bacteria: to co-transcriptionally separate

213 nt evidence for cytoplasmic translocation of RNase III nucleases in response to virus in diverse euka

214 gene-loss events to generate the variety of RNase III enzymes found in modern-day budding yeasts.

215 ze substrate specificity and product size of RNase IIIs, we performed in vitro cleavage of dsRNAs by

216 ls in mutants defective in either RNase E or RNase III.

217 hydrolysis that can be extrapolated to other RNase III family members.

218 Prokaryotic RNase III enzymes have been well characterized, but how

219 ditional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.

220 KREPB1 with point mutations in the putative RNase III catalytic domain also blocked growth, in vivo

221 dependent, factors that dynamically regulate RNase III actions during normal cell growth.

222 Here we demonstrate that Drosha and related RNase III ribonucleases from all three domains of life a

223 Members of the ribonuclease (RNase) III family regulate gene expression by processing

224 Drosha is a member of the ribonuclease (RNase) III family that selectively processes RNAs with p

225 onuclease required in DNA repair, and Rnt1p (RNase III), an endoribonuclease required for RNA maturat

226 malian CNS, we used mice in which the second RNase III domain of Dicer was conditionally floxed.

227 n primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the ba

228 Each editosome requires the single RNase III domain in each endonuclease for catalysis.

229 tion intermediate against the dsRNA-specific RNase III.

230 The tandem RNase III (RIII) domains of Dcr-1 form an intramolecular

231 Dicer-2 contains C-terminal RNase III domains that mediate RNA cleavage and an N-ter

232 NAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box

233 amount of dsRNA is formed in the cell, that RNase III degrades or processes these dsRNAs, and that d

234 We find that RNase III rapidly and efficiently cleaved the transcript

235 Previously, we have shown that RNase III uses two catalytic sites to create the 2-nucle

236 nce and structure analyses, we conclude that RNase IIIs recognize +3G via a conserved glutamine (EcQ1

237 The RNase III endonuclease Dicer plays a key role in generat

238 The RNase III enzyme Dicer processes RNA into siRNAs and miR

239 The RNase III enzyme Drosha initiates microRNA (miRNA) bioge

240 The RNase III gene of Streptomyces coelicolor, which was dis

241 The RNase III polypeptide contains an N-terminal catalytic (

242 sha processing is currently unique among the RNase III enzymes.

243 8 is an RNA-binding protein that assists the RNase III enzyme Drosha in the processing of microRNAs (

244 These pre-miRNAs are cleaved by the RNase III Dicer to generate mature miRNAs that direct th

245 miRNA is processed from the pre-miRNA by the RNase III Dicer.

246 erminal tRNA-derived fragments (tRFs) by the RNase III endonuclease Dicer, we show that several RNase

247 partially double-stranded precursors by the RNase III endonucleases Drosha and Dicer, thereby genera

248 al microRNA biogenesis pathway driven by the RNase III enzymes Drosha and Dicer, an unexpected variet

249 taining RNA polymerase II transcripts by the RNase III enzymes Drosha in the nucleus and Dicer in the

250 onical miRNAs is sequentially cleaved by the RNase III enzymes, Drosha and Dicer, which generate 5' m

251 gions whose expression was influenced by the RNase III gene deletion.

252 ce with a conditional deletion of Dicer, the RNase III endonuclease that produces mature microRNAs in

253 and cd19-Cre-mediated deletion of Dicer, the RNase III enzyme critical for generating mature miRNAs.

254 short interfering RNA (siRNA) by DICER, the RNase III enzyme that initiates RNAi in human cells.

255 presence and absence of Dicer or Drosha, the RNase III nucleases responsible for generating small RNA

256 by RNase III and Dicer are missing from the RNase III domains in RNC1.

257 onal roles of two divalent metal ions in the RNase III catalytic mechanism.

258 functions by interacting with a site in the RNase III catalytic region, that expression of YmdB is t

259 ations in DICER1, an endoribonuclease in the RNase III family that is essential for processing microR

260 eles with single amino acid mutations in the RNase III motif had similar consequences.

261 lly functional asRNAs were identified in the RNase III mutant strain and are encoded primarily opposi

262 levels were higher or detectable only in the RNase III mutant strain.

263 tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae

264 r than the K(Mg) of RNase III (0.46 mM), the RNase III[E41A/D114A] double mutant has a K(Mg) of 39 mM

265 Deficient expression of the RNase III DICER1, which leads to the accumulation of cyt

266 -stranded RNA-binding protein partner of the RNase III enzyme Drosha.

267 Rnt1p, a member of the RNase III family of dsRNA endonucleases, is a key compon

268 ge is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcript

269 of miR166g, and require the activity of the RNase III helicase DCL1.

270 Deletion or mutation of the RNase III motif abolished this activity.

271 ous work demonstrated the involvement of the RNase III-like endoribonuclease, Bs-RNase III, in scRNA

272 The implications of these findings on the RNase III catalytic mechanism are discussed.

273 Essential insight on the RNase III mechanism of dsRNA cleavage has been provided

274 rabidopsis indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein

275 It is shown here that the RNase III[E41A] and RNase III[D114A] mutants exhibit cat

276 for siRNA-directed mRNA cleavage, though the RNase III activity of Dicer-2 is not required.

277 Our results suggest two models whereby the RNase III enzymes of a fungal ancestor, containing both

278 y miRNAs (pri-miRNAs) in connection with the RNase III enzyme Drosha.

279 ing in the context of a heterodimer with the RNase III-domain protein RNC1.

280 Seven of these were within the RNase III domain, and two were in the C-terminal region

281 During viral infection, the RNase-III-type endonuclease Dicer cleaves viral double-s

282 T. maritima (Tm) RNase III catalytic activity exhibits a broad optimal te

283 Tm-RNase III cleavage of substrate is optimally supported b

284 stem sequences are efficiently cleaved by Tm-RNase III at sites that are consistent with production i

285 In fact, Tm-RNase III cleaves an Ec-RNase III substrate with identic

286 s (miRNAs) are sequentially processed by two RNase III enzymes, Drosha and Dicer.

287 latory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer.

288 An involvement of two RNase III-containing core editing complex (L-complex) pr

289 ecent report in Cell reveals how Dicer's two RNase III domains collaborate during dsRNA processing an

290 and cold shock adaptation are dependent upon RNase III cleavage of an rpsO-pnp intergenic hairpin.

291 Biogenesis of CoV svRNAs was RNase III, cell type, and host species independent, but

292 s in the db to inhibit cleavage by weakening RNase III binding indicates that the db is a positive de

293 e Arg95 guanidinium group, thereby weakening RNase III engagement of product.

294 While RNase III[E41A] and RNase III[D114A] have K(Mg) values t

295 +) concentrations for optimal activity, with RNase III[E100A] exhibiting the largest K(Mg).

296 l to the nucleus and becomes associated with RNase III DROSHA and the RNA helicase p68.

297 and addition, 2 RNA ligases, 2 proteins with RNase III-like domains, and 6 proteins with predicted ol

298 Five related proteins with RNase III-like motifs also contain a U1-like zinc finger

299 Comparison of PARE peaks in strains with RNase III present or absent showed that, in addition to

300 ovel polypeptides, among which were two with RNase III, one with an AP endo/exonuclease and one with