ライフサイエンスコーパス: RPS6

1 that of phosphorylated ribosomal protein S6 (RPS6).

2 ced phosphorylation of ribosomal protein S6 (RPS6).

3 in 1 (4E-BP1), but not ribosomal protein S6 (rpS6).

4 ion of mTOR signaling and phosphorylation of rpS6.

5 a ribosomal protein (rp)S6 kinase, S6K1, and rpS6.

6 ressed into soybean lines along with Rps4 or Rps6.

7 ation of Thr(389) and of the S6K1 substrate, RPS6.

8 to inhibition of the kinase as compared with rpS6.

9 4E-BP1 but also inhibited phosphorylation of rpS6.

10 or of parathyroid cell proliferation through rpS6.

11 slation through phosphorylation of LARP1 and rpS6.

12 sed phosphorylation of ribosomal protein S6 (rpS6), a downstream target of the mTOR pathway.

13 RPS6, a component of the mammalian target of rapamycin (

14 osophila melanogaster cells expressing human RPS6, a TORC1 effector whose phosphorylated form we dete

15 locked diurnal oscillations in eIF4E, 4EBP1, rpS6, Akt, and ERK1/2 phosphorylation and impaired memor

16 the mammalian target of rapamycin complex 1/rpS6/Akt1/2 signaling pathway to support NC1 peptide-med

17 se (RPS6KB1), and phosphorylated S6 protein (RPS6), all of which mediate cell growth and proliferatio

18 Here, we focus on ribosomal protein S6 (rpS6), an mTOR effector not implicated previously in can

19 locked UNX-increased phosphorylation of both rpS6 and 4E-BP1.

20 ts and partially restored phosphorylation of RPS6 and 4EBP1.

21 Additionally, we discovered that p70/RPS6 and 4EBP1/eIF4E acted downstream of mTORC1, regulat

22 We propose that the interaction between RPS6 and AtHD2B brings about a change in the chromatin s

23 Co-expression of RPS6 and AtHD2B caused a change in the location of both

24 HD2B caused a change in the location of both RPS6 and AtHD2B to one or several nucleolar spots.

25 RPS6 and AtHD2B were localized to the nucleolus.

26 In conclusion, the cloning of RPS6 and comparisons with RPS4 will contribute to a clos

27 sphorylation of S6 kinase and its substrates rps6 and eEF2 kinase that lead to dephosphorylation of e

28 ose-stimulated phosphorylation of S6 kinase, rps6 and eEF2 kinase, and inhibited the dephosphorylatio

29 The mTORC1 effectors, RPS6 and eIF4E, play distinct roles and are both necessa

30 chemical inhibitor, or knockdown of AKT1S1, RPS6 and EIF4EBP1 expression by small interfering RNA, m

31 mTORC1 signaling pathway, i.e. AKT, AKT1S1, RPS6 and EIF4EBP1, was upregulated in islets upon TGFBI

32 the phosphorylation of two S6K1 substrates, rpS6 and eukaryotic initiation factor 4B, was not repres

33 0S6K activation inhibited phosphorylation of RPS6 and IL-3-enhanced semaphorin-7A translation.

34 ivated MAPK signaling and phosphorylation of rpS6 and led to phosphorylation of GSK3beta-Ser(9), a no

35 ghts into the impact of the positive TOR-S6K-RPS6 and negative TOR-MAF1 modules on translation repres

36 c pair of NDDs, and uncouples the roles of p-RPS6 and p-4EBP as mechanistically actionable relays in

37 mTOR pathway as well as distinct roles of p-RPS6 and p-4EBP during neurogenesis.

38 phosphorylation of the p-AKT1, p-EIF4EBP1, p-RPS6 and p-EIF4E signaling proteins.

39 In addition, RPS6 and p70S6 kinase were hypophosphorylated, while the

40 nged environment, in vivo phosphorylation of RPS6 and p90S6K was enhanced in human airway compared wi

41 mediated by the resistance genes RPS2, RPS4, RPS6 and RPM1.

42 lysed for protein content of AKT/mTOR/p70S6K/rpS6 and their corresponding phosphorylation sites, REDD

43 -NB-LRR disease resistance proteins RPS4 and RPS6 and with the negative immune regulator SRFR1 at a c

44 its role in ribosome assembly suggests that RPS6 (and by extension other ribosomal proteins) contrib

45 s by regulating p70S6K/ribosomal protein S6 (RPS6) and eukaryotic translation initiation factor 4E-bi

46 easome activity; phosphorylation of S6K1 and rpS6) and rapamycin-insensitive (phosphorylation of eEF2

47 t nsP2 associates with ribosomal protein S6 (RpS6) and that nsP2 is present in the ribosome-containin

48 the phosphorylation of ribosomal protein S6 (rpS6) and the amount of phosphorylated rpS6 bound to the

49 phorylation of the 40S ribosomal protein S6 (rpS6) and the eukaryotic translation initiation factor (

50 through activation of ribosomal protein S6 (RPS6) and the upstream kinase 90-kDa ribosomal S6 kinase

51 sed ERK1/2, MAPK-activated protein kinase 2, rpS6, and CREB phosphorylation in fetal Tbet(+)CD4(+) T

52 on of mTOR and its effectors 4E-BP1, p70S6K, rpS6, and eukaryotic initiation factor 4G.

53 ns with Myo5a, Prkra (PACT), Gnb2l1 (RACK1), Rps6, and Syt2 were confirmed by Western blot analysis.

54 As both RSK and rpS6 are known to be important for cell proliferation an

55 all inhibitory RNA knockdown of RPS6 defined RPS6 as a critical regulator of 5' TOP translation.

56 we addressed the role of phosphorylation of rpS6 as an effector of mTOR function in T cell developme

57 Our results show that phosphorylation of RPS6 as well as 4E-binding protein 1 (4EBP1) was reduced

58 hosphorylation of S6 kinase and its effector rpS6, as well as phosphorylation of the translational re

59 For the first time, we show that RPS6 associates with multiple mRNAs containing a 5' TOP

60 , there was an increase in the activation of rpS6 at rest.

61 nd forskolin, lead to the phosphorylation of rpS6 at Ser235/Ser236 independently of the activation of

62 ls, phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 was mostly abrogated, and this BMK1-

63 Here we report the identification of RPS6 based on a forward-genetic screen and map-based clo

64 ating that the corresponding resistance gene RPS6 belongs to the TNL class.

65 n S6 (rpS6) and the amount of phosphorylated rpS6 bound to the translation initiation complex were in

66 nsP1 also was associated with RpS6, but other nonstructural proteins were not.

67 that protein phosphatase 1 (PP1) antagonizes rpS6 C terminus phosphorylation and cap binding in intac

68 Knockin rpS6(p-/-) mice, in which rpS6 cannot be phosphorylated because of substitution of

69 and Ser-244) whose modification potentiates rpS6 cap binding activity.

70 pression and the activity of MEK-ERK and S6K-RPS6 cascades but also displays a potent antiproliferati

71 come anabolic signaling deficits (mTORC1 and rpS6), corroborating the greater postprandial aminoacide

72 inishes phosphorylation of eIF4B, eIF4E, and rpS6, critical components of the intracellular machinery

73 h specific small inhibitory RNA knockdown of RPS6 defined RPS6 as a critical regulator of 5' TOP tran

74 ChIP analysis suggests that RPS6 directly interacts with the rRNA gene promoter.

75 expressing a nonphosphorylatable variant of Rps6 display a reduced growth rate and a 40S biogenesis

76 liferation, in the renal tubules of Tsc1 and rpS6 double-mutant mice.

77 onsistently, we found phosphorylated RPS6 (p-RPS6) downregulated in WBS and upregulated in 7Dup.

78 otein S6 kinase (S6k), ribosomal protein S6 (rpS6), eukaryotic elongation factor 2 (eEF2), and eukary

79 ther, we conclude that PKA can phosphorylate rpS6 exclusively at Ser235/Ser236 in vivo in pancreatic

80 Protoplasts overexpressing both AtHD2B and RPS6 exhibited down-regulation of pre-18 S rRNA synthesi

81 Depletion of RPS6 from primary effusion lymphoma (PEL) cells dramatic

82 of the mTOR signaling pathway that regulate rpS6 function.

83 Similar to RPS4 and other TNL genes, RPS6 generates alternatively spliced transcripts, althou

84 Two maize (Zea mays) rps6 genes were identified that encode polypeptides (30

85 The fact that RPS6 has a well-established nuclear function beyond its

86 tion of mTOR1 can suppress both the observed RPS6 hyperphosphorylation and increased cell size.

87 We observed ribosomal protein S6 (RPS6) hyperphosphorylation as a shared signaling alterat

88 diminished expression and phosphorylation of RPS6, impacting ribosomal protein synthesis, and a decre

89 des the translational machinery, implicating RPS6 in 5' TOP translation.

90 OS or NIH 3T3 cells phosphorylates mammalian RPS6 in a mitogen-dependent wortmannin- and rapamycin-se

91 BP1, eIF2alpha, AMPKalpha, p70 S6 kinase and rpS6 in muscle homogenates.

92 tle is known about other possible role(s) of RPS6 in plants, besides being a component of the 40 S ri

93 adient, suggesting that nsP2 associates with RpS6 in the context of the whole ribosome.

94 Finding a role for RpS6 in the negative regulation of efferocytosis provide

95 Notably, knockin of a nonphosphorylatable rpS6 in these Tsc1-mutant mice exacerbated cystogenesis

96 presence of BMK1 and the phosphorylation of rpS6 in tumor-associated endothelial cells of blood vess

97 sed phosphorylation of ribosomal protein S6 (rpS6) in activated renal tubules.

98 d phosphorylation of ribosomal protein S6 (p-rpS6) in SNpc neurons, a readout of trkB activation.

99 protein 1 (RCCD1) and ribosomal protein S6 (RPS6) in vitro.

100 K1) and its substrate, ribosomal S6 protein (rpS6), in quiescent 3T3 cells.

101 apamycin (mTOR) effectors 4EBP1, p70S6K, and rpS6, independent of HIF.

102 of the ribosomal proteins, such as RPL24 and RPS6, inhibits polysome assembly by stabilizing eIF6 bin

103 ther nonstructural proteins was required for RpS6 interaction with nsP2.

104 Here, we show that ribosomal protein S6 (RPS6) interacts with LANA.

105 RPS6 is a potential novel disease-related gene.

106 Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subu

107 Ribosomal protein S6 (rpS6) is an essential component of the ribosome and is i

108 y, we demonstrate that ribosomal protein S6 (RPS6) is highly expressed in primary diffuse large B-cel

109 Ribosomal protein S6 (RPS6) is located in the mRNA binding site of the 40S sub

110 The ribosomal protein S6 (RPS6) is one of the well known downstream components of

111 Ribosomal protein S6 (rpS6) is phosphorylated in vivo by isoforms of p70 S6 pr

112 Nine RPS6 isoforms were resolved in a two-dimensional basic-u

113 This cAMP-dependent rpS6 kinase activity is also sensitive to PKI in vitro,

114 henotypes are not observed in cells in which Rps6 kinase activity is compromised.

115 d phosphorylation of the ERK1 and the 90-kDa rpS6 kinase p90(rsk).

116 Treatment with rapamycin or an RPS6-kinase inhibitor (LY2584702) suppressed RPS6 phosph

117 he activation of the currently known in vivo rpS6 kinases via a pathway that is sensitive to inhibito

118 disorder genotypes, inhibition of mTORC1 or RPS6 kinases warrants further investigation as potential

119 osphorylation (either by generating congenic rpS6 knock-in mice expressing non-phosphorylatable rpS6

120 and demonstrates that observations made with Rps6 knock-ins must be interpreted cautiously.

121 Indeed, rpS6 knockin mice are completely sensitive to the inhibi

122 ss of SNpc neuron, nor did STN DBS elevate p-rpS6 levels further.

123 reatment with an inhibitor increased phospho-rpS6 levels, promoted podocyte hypertrophy and exacerbat

124 Here, we report that RPS6 may have a novel function via interaction with hist

125 additionally show that TOR function involves RPS6-mediated nutrition and light-dependent growth and l

126 A reduction in RPS6 mRNA capping, protein expression, and phosphorylati

127 The rpS6 mutants expressing oncogenic Kras showed increased

128 R1 also enhanced HopA1-triggered immunity in rps6 mutants.

129 - and loss-of-function Ribosomal Protein S6 (RPS6) mutants additionally show that TOR function involv

130 of phosphorylated (p)-ribosomal protein S6 (rpS6) (namely, p-rpS6-S235/S236) and a concomitant down-

131 predicted secondary structure similarity to RPS6 of other eukaryotes.

132 d PKA exclusively phosphorylates recombinant rpS6 on Ser235/Ser236 in vitro.

133 nce that PKA is also likely to phosphorylate rpS6 on Ser235/Ser236 in vivo in a number of other mamma

134 nock-in mice expressing non-phosphorylatable rpS6 or by inhibiting ribosomal protein S6 kinase 1-medi

135 similar effect is observed upon depletion of RPS6 or RPL11.

136 ts activated downstream targets p-RPS6KB1, p-RPS6, or p-EIF4EBP1 was associated with adverse clinical

137 es of resistance to P. sojae, such as Rps3a, Rps6, or Rps11, into commercial cultivars to effectively

138 uronal migration delay and cytomegaly, while RPS6 p.R232H prompted increased cell proliferation.

139 not change the expressions of Akt (p = 0.9), rpS6 (p = 0.3), Foxo3a (p = 0.12) and anti-apoptotic Bcl

140 Consistently, we found phosphorylated RPS6 (p-RPS6) downregulated in WBS and upregulated in 7D

141 Uremic rpS6(p-/-) mice had no increase in parathyroid cell prol

142 five phosphorylatable sites in rpS6 (termed rpS6(P-/-) mice).

143 Knockin rpS6(p-/-) mice, in which rpS6 cannot be phosphorylated

144 r DMBA or mutant Kras was greatly reduced in rpS6(P-/-) mice.

145 hes, we identified the ribosomal protein S6 (RPS6) p.R232H variant, present as somatic mosaicism at ~

146 Here, we show that inhibition of the RPS6 pathway by rapamycin effectively suppressed, wherea

147 mTOR complex 1/ribosomal protein S6 (mTORC1/RPS6) pathway as well as the reduced expression of sever

148 These findings further our understanding of rpS6 phospho-regulation and define a direct link between

149 Importantly, blocking rpS6 phosphorylation (either by generating congenic rpS6

150 trate that Csnk1a1 inhibition causes reduced Rps6 phosphorylation and activation of p53, resulting in

151 of acinar ductal metaplasia, suggesting that rpS6 phosphorylation attenuates Kras-induced DNA damage

152 dence indicating that specifically targeting rpS6 phosphorylation can attenuate the development of FS

153 rscore the importance of mTOR activation and rpS6 phosphorylation for the pathogenesis of secondary h

154 with rpS6 phosphorylation, thus dissociating rpS6 phosphorylation from the translational control of t

155 3-acetate, known activators of PKC, leads to rpS6 phosphorylation in a rapamycin-dependent manner.

156 RPS6-kinase inhibitor (LY2584702) suppressed RPS6 phosphorylation in both, suggesting upstream activa

157 depicts the signaling cascades orchestrating Rps6 phosphorylation in budding yeast, challenges the no

158 duced cell size and dose-dependently reduced RPS6 phosphorylation in E420K-variant cells, suggesting

159 me profiling, we failed to uncover a role of Rps6 phosphorylation in either global translation or tra

160 e model of FSGS, we investigated the role of rpS6 phosphorylation in podocyte hypertrophy and loss du

161 However, the role of rpS6 phosphorylation in podocyte hypertrophy and podocyt

162 These results reveal that rpS6 phosphorylation is important for the initiation of

163 Furthermore, PMT-induced rpS6 phosphorylation is inhibited by PKC inhibitor, Go69

164 Surprisingly, we demonstrate that rpS6 phosphorylation is not required for any of these pr

165 Here, we examined rpS6 phosphorylation levels in kidney biopsy specimens f

166 cerevisiae, we found that the regulation of Rps6 phosphorylation on Ser-232 and Ser-233 is mediated

167 in budding yeast, challenges the notion that Rps6 phosphorylation plays a role in translation, and de

168 Heterogeneity in RPS6 phosphorylation was a consequence of the presence o

169 RpS6 phosphorylation was dramatically diminished within

170 as insulin-like growth factor I receptor and RPS6 phosphorylation were enriched in the "proliferation

171 iting ribosomal protein S6 kinase 1-mediated rpS6 phosphorylation with an inhibitor) significantly bl

172 genes as significant on-target regulators of RPS6 phosphorylation, and we characterized them with ext

173 that Csnk1a1 knockdown results in decreased Rps6 phosphorylation, increased p53 activity, and myeloi

174 ough regulatory mechanisms such as eIF4E and rpS6 phosphorylation, mediated by activation of the ERK1

175 B and mTOR activity, as measured by p-65 and RPS6 phosphorylation, respectively, and upregulated the

176 n target of rapamycin complex 1 signaling to rpS6 phosphorylation, resulting in podocyte hypertrophy

177 us administration of IGF-1 rescued defective rpS6 phosphorylation, spine density, and PSD-95 expressi

178 oligopyrimidine tract, did not coincide with rpS6 phosphorylation, thus dissociating rpS6 phosphoryla

179 hosphatase 1 is known to negatively regulate rpS6 phosphorylation, treatment with an inhibitor increa

180 To examine the functional significance of rpS6 phosphorylation, we used knockin mice lacking all f

181 size, insulin levels, glucose tolerance, and RPS6 phosphorylation, without rescuing IUGR.

182 inal phospho-residues is required to sustain rpS6 phosphorylation.

183 kinase(s) determine complex heterogeneity in RPS6 phosphorylation.

184 osmotic stress had no reproducible effect on RPS6 phosphorylation.

185 ion independently of its ability to regulate rpS6 phosphorylation.

186 tivation, it exerts no effect on PMT-induced rpS6 phosphorylation.

187 aradoxical increase in ribosomal protein S6 (rpS6) phosphorylation and a decrease in eukaryotic initi

188 Ribosomal protein S6 (rpS6) phosphorylation mediates the hypertrophic growth o

189 of MAPK signaling and the phosphorylation of rpS6 produced by D1 activation in D1-MSNs, paralleling b

190 this idea, CK1-dependent phosphorylation of rpS6 promotes its association with the mRNA cap-binding

191 Furthermore, a reduction in the level of RpS6 protein expression led to diminished expression fro

192 Genetic modulation of RPS6 protein levels with specifically targeted short hai

193 tracellular signal-regulated kinase) and S6K-RPS6 (ribosomal protein S6 kinase-ribosomal protein S6)

194 n- and stress-activated protein kinase 1 and rpS6 (ribosomal protein S6) was largely unchanged under

195 IL-5 or GM-CSF in maintaining the ERK/p90S6K/RPS6 ribosome-directed signaling pathway, leading to inc

196 0, RPS12, RPS18, RPL30, RPS20, RPL12, RPL7A, RPS6, RPL27A, RPLP2, RPS25, RPS3, RPL41, RPL6, RPLP0, RP

197 ation increased its association with PABPC4, RPS6, RPL7, and other translational factors.

198 ction of intron-encoded nascent peptides and RPS6/RPL7-carrying complexes in the perinucleolar compar

199 Here, we show that three resistance genes, Rps6, Rps7, and Rps8, contribute to immunity in barley t

200 on of p-Akt1-S473 and p-Akt2/S474, but not p-rpS6-S235/S236 up-regulation.

201 d (p)-ribosomal protein S6 (rpS6) (namely, p-rpS6-S235/S236) and a concomitant down-regulation of p-A

202 ondition interaction effects revealed that p-rpS6(S240/244) was greater in DEHY than EUHY at PRE and

203 orylation sites in a protein sequence (e.g., RPS6 S245) which did not appear in the training dataset,

204 ic Thr389 residue but not at Thr421/Ser424), rpS6 (Ser235/236) and 4E-BP1 (gel shift), as well as def

205 phorylation on Thr389 and phosphorylation of rpS6 (ser235/36), suggesting p70S6K kinase activity was

206 Immunoblotting for rpS6 showed modest polysomal disaggregation upon fasting

207 function, the deduced amino acid sequence of RPS6 shows highest similarity to the TNL resistance prot

208 Our screen revealed that the TORC1-S6K-RPS6 signaling axis is regulated by many subcellular com

209 hrough its role in the regulation of the RSK-rpS6 signaling module.

210 gh the alternative transcript structures are RPS6 specific.

211 s in RICTOR (25% of patients) and positive p-RPS6 staining correlated with recurrence.

212 the phosphorylation of ribosomal protein S6 (rpS6), suggesting activation of the ribosomal S6 kinases

213 bit phosphorylation of ribosomal S6 protein (rpS6), suggesting the reduced activity of mammalian targ

214 ing mTORC1 signaling, whereas phosphorylated rpS6 suppresses cystogenesis and fibrosis in Tsc1-delete

215 e lacking all five phosphorylatable sites in rpS6 (termed rpS6(P-/-) mice).

216 ts with phosphorylated ribosomal protein S6 (RpS6) to promote its ubiquitylation and proteasomal degr

217 In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70

218 Maize RPS6 was analyzed by the use of two-dimensional gel elec

219 olysome fractions showed that phosphorylated rpS6 was disproportionately present in translating polys

220 Phosphorylated rpS6 was found to be markedly increased in the podocytes

221 Phosphorylation of rpS6 was increased in pancreatic acinar cells upon impla

222 Aberrant mTOR signaling (p-RPS6) was present in half of the cases, associated with

223 ins the key regulatory ribosomal protein S6 (rpS6), we considered that myc loss might affect expressi

224 IF3A, eIF4G, and small ribosomal protein S6 (rpS6), were redistributed into foci located in the same

225 ts of the PI3K/mTOR pathway, MLST8, 4EBP and RPS6, were significant TSSs in productively infected cel

226 naling-related proteins, including a site on RPS6 with a decrease in phosphorylation.

227 The association of RpS6 with nsP2 was detected throughout the course of inf