ライフサイエンスコーパス: RdRP

1 RdRP and E RT-RPA assays detected SARS-CoV-1 and 2 genom

2 RdRP mice also had considerably lower Friend retrovirus

3 RdRP, E, and N RT-RPA assays required approximately 15 m

4 RdRP-transgenic mice (RdRP mice) resist infection and di

5 RdRPs are targets for antiviral drug development, but th

6 RdRPs have been proposed to act through a variety of mec

7 Our study shows that when abundant RdRP-produced siRNAs are depleted, there is ectopic elev

8 by an RNA-dependent RNA polymerase activity (RdRP).

9 tions in conserved motif A drastically alter RdRP fidelity, which can be either increased or decrease

10 We show that orthologs of QDE-1, an RdRP component of the quelling pathway in Neurospora cra

11 ng evidence that mammalian Pol II acts as an RdRP to control the stability of a cellular RNA by exten

12 e is the first example of a gene encoding an RdRP-related protein with an essential developmental fun

13 R-1 Argonaute require the DRH-3 helicase, an RdRP component.

14 al development to adulthood in wild-type and RdRP mice.

15 d shell protein (CSP) interacts with VP4 and RdRP.

16 tion restricts movements of the fingers, and RdRPs use a subtle conformational change within the palm

17 Both RdRP recruitment and mRNA silencing require Argonaute pr

18 es from an independent de novo initiation by RdRP.

19 d by sequential use of downstream primers by RdRP.

20 ipts derived from cellular messenger RNAs by RdRP activity may have key roles in cellular regulation.

21 undant viral and host siRNAs by the cellular RdRPs.

22 ong different viruses by generating chimeric RdRPs from poliovirus and coxsackievirus B3.

23 riptomics data based on the highly conserved RdRP protein, enabling the detection of over 100,000 RNA

24 polymerase (RdRP) outside the viral context (RdRP mice) exhibit constitutive, MDA5-dependent, and qua

25 We have previously shown that this core RdRP structure and mechanism provide a platform for poly

26 nscriptional silencing using a single Dicer, RdRP, and Argonaute protein.

27 tes tightly with Dcr-2, similar to the Dicer-RdRP interaction observed in lower eukaryotes.

28 e for successive engagement of two different RdRPs in an endogenous siRNA-based mechanism targeting s

29 dsRNA from the same source or use different RdRPs to perform the same function.

30 Here, we show that distinct RdRPs function sequentially to produce small RNAs that t

31 yze the function of a Caenorhabditis elegans RdRP, RRF-3, during spermatogenesis.

32 There are four C. elegans RdRPs, including two with known germline functions.

33 Among C. elegans RdRPs, we find that only EGO-1 is required for H3K9me2 e

34 cDNAs representing only two genes encoding RdRPs were identified in N. benthamiana.

35 Extended RdRP phylogeny supports the monophyly of the five establ

36 Multiple active forms of the FCV RdRP were identified.

37 ng that Pro-Pol is an active form of the FCV RdRP.

38 al-time RT-PCR (n = 36) were 94 and 100% for RdRP; 65 and 77% for E; and 83 and 94% for the N RT-RPA

39 e importance of the palm domain movement for RdRP active site closure and demonstrate that protein en

40 ining new interactions that are required for RdRP activity.

41 l highlighting the key features required for RdRP inhibition is proposed.

42 which converts these RNAs into templates for RdRP-based antiviral siRNA production, thereby limiting

43 nteraction with L to form a fully functional RdRP.

44 fied and the cellular function of the Pol II RdRP activity is unknown.

45 lting from extension of B2 RNA by the Pol II RdRP can be removed from Pol II by a factor present in n

46 In RdRP mice, the proportion of upregulated ISGs increased

47 at, in the drh-3(ne4253) mutant deficient in RdRP-produced secondary endo-siRNAs, the silencing histo

48 We report the occurrence of a mutation in RdRP (D484Y) following treatment with remdesivir in a 76

49 res newly synthesized RdRP and that incoming RdRP can only generate mRNA.

50 The individual RdRP subcomplex contains all the characterized motifs an

51 equence to the salicylic acid (SA)-inducible RdRP from Nicotiana tabacum required for defense against

52 iana plants transformed with an SA-inducible RdRP gene from Medicago truncatula were more resistant t

53 hese results strongly suggest that inducible RdRP activity plays an important role in plant antiviral

54 iana lacks an active SA- and virus-inducible RdRP and thus is hypersusceptible to viruses normally li

55 es differed from those of other SA-inducible RdRPs in that they contained a 72-nt insert with tandem

56 We infected RdRP mice and wild-type (WT) mice with various doses of

57 During chronic infection, RdRP mice had 2.35 +/- 0.66 log(10) lower circulating vi

58 es an intimate association between influenza RdRP and cellular RNA polymerase II (Pol II), which is t

59 itiation of viral transcription by influenza RdRP.

60 ructural studies have revealed how influenza RdRP binds to Pol II and how this binding promotes the i

61 he biochemical characterization of influenza RdRP subcomplex comprising PA, PB1, and N terminus of PB

62 , as both a substrate and a template for its RdRP activity.

63 tein of RABV and the characterization of its RdRP activity in vitro The study provides a new assay th

64 system for study of this process, since its RdRP (VP1) is catalytically active and can specifically

65 to the N-RNA template and orchestrate the L(RdRP), L(PRNT), and L(MT), an oligomeric phosphoprotein

66 tion of a capping enzyme with a picorna-like RdRP in the AfuTmV-1 genome is a striking case of chimer

67 We identified viruses encoding HsRV-like RdRPs in marine water, river sediments and salt marshes,

68 RdRP-transgenic mice (RdRP mice) resist infection and disease caused by severa

69 Compared to WT mice, RdRP mice had significantly reduced splenomegaly, viral

70 D-elp1 is a noncanonical RdRP that can synthesize dsRNA from different ssRNA temp

71 Second, the NS5 RdRP domain also binds the amino-terminal domain of hSTA

72 core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as esse

73 Due to the general conservation of RdRP structures, these results suggest that the specific

74 We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV i

75 n conclusion, the upregulated ISG profile of RdRP mice is mostly triggered early postnatally, is main

76 ine the requirements in P for stimulation of RdRP activity as residues 11 to 50 of P and formally dem

77 teraction between the PA and PB1 subunits of RdRP, we have designed and synthesized a series of analo

78 To further our understanding of RdRP function, we assembled, purified, and then crystall

79 g a new space in the chemical variability of RdRP inhibitors.

80 enger RNA (mRNA) leads to the recruitment of RdRPs and synthesis of secondary siRNAs using the target

81 plication of SARS-CoV-2 via direct action on RdRP, with both models being useful.

82 to the active site had only minor effects on RdRP function, but the stacking interaction between Phe(

83 esis and the consequences of these events on RdRP function.

84 One RdRP was similar in sequence to SDE1/SGS2 required for m

85 igonucleotides specific for either N gene or RdRP gene that can form silver nanoclusters (AgNCs) with

86 We conclude that divergence of orthologous RdRPs can result in functional innovation while retainin

87 lls, copurifying proteins included the other RdRP subunits (PB1 and PA) and the viral nucleoprotein a

88 iral siRNAs by RNA-dependent RNA polymerase (RdRP) 1 (RDR1) and RDR6 and of the endogenous virus-acti

89 Plants contain RNA-dependent RNA polymerase (RdRP) activities that synthesize short cRNAs by using ce

90 ransferase and RNA-dependent RNA polymerase (RdRP) activities.

91 regulating the RNA-dependent RNA polymerase (RdRP) activity of NSP12.

92 ported to have RNA-dependent RNA polymerase (RdRP) activity.

93 r complex, has RNA-dependent RNA polymerase (RdRP) activity.

94 g subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4.

95 ies include an RNA-dependent RNA polymerase (RdRP) and an RNA endonuclease that cleaves capped primer

96 ing a putative RNA-dependent RNA polymerase (RdRP) and coat protein (CP), respectively, as confirmed

97 regenerated by RNA-dependent RNA polymerase (RdRP) and Dicer, but siRNAs from single-copy sequences a

98 ubstrate of PV RNA-dependent RNA polymerase (RdRP) and is incorporated into RNA mimicking both ATP an

99 d to the tomato RNA-directed RNA polymerase (RdRP) and to Neurospora crassa QDE-1, two proteins impli

100 y and in vitro RNA-dependent RNA polymerase (RdRP) assays that AVG compounds bind to the viral polyme

101 the large (L) RNA-dependent RNA polymerase (RdRP) bound to a nucleoprotein (NP)-encapsidated genomic

102 nfluenza virus RNA-dependent RNA polymerase (RdRP) cleaves the 5' end of nascent capped host RNAs and

103 that the viral RNA-dependent RNA polymerase (RdRP) complex can be an optimal target of protein-protei

104 e component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA s

105 s) produced by RNA-dependent RNA polymerase (RdRP) complexes.

106 ich encodes an RNA-dependent RNA polymerase (RdRP) containing a unique GDNQ motif normally characteri

107 ransferase and RNA-dependent RNA polymerase (RdRP) domains form a conserved interdomain cleft harbori

108 her csr-1, the RNA-dependent RNA polymerase (RdRP) ego-1, or the dicer-related helicase drh-3, leads

109 including the RNA-dependent RNA polymerase (RdRP) EGO-1, the Dicer-related helicase DRH-3, the Tudor

110 which recruit RNA-dependent RNA polymerase (RdRP) enzymes that drive gene silencing by synthesizing

111 R6, a putative RNA-dependent RNA polymerase (RdRP) from Arabidopsis thaliana, has previously been fou

112 lly within the RNA-dependent RNA polymerase (RdRP) gene.

113 an analysis of RNA-directed RNA polymerase (RdRP) hallmark gene sequences of flaviviruses and 'flavi

114 The RNA-dependent RNA polymerase (RdRP) of nonsegmented negative-sense RNA viruses consist

115 assay for the RNA-dependent RNA polymerase (RdRP) of rabies virus (RABV).

116 ly inhibit the RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2.

117 e of the viral RNA-dependent RNA polymerase (RdRP) on host factors makes it a major host range determ

118 he error-prone RNA-dependent RNA polymerase (RdRP) or through genetic reassortment enables perpetuati

119 a picornavirus RNA-dependent RNA polymerase (RdRP) outside the viral context (RdRP mice) exhibit cons

120 nts coding for RNA-dependent RNA Polymerase (RdRP) produced more vRNA than mRNA while some other segm

121 thesized by an RNA-dependent RNA polymerase (RdRP) requires the PIR-1 phosphatase.

122 The viral RNA-dependent RNA polymerase (RdRP) resides within an approximately 250-kDa large prot

123 by poliovirus RNA-dependent RNA polymerase (RdRP) revealed that 3-NPNTP was not accepted universally

124 ext, the viral RNA-dependent RNA polymerase (RdRP) subunits assembly has emerged as an attractive tar

125 y of a targeted RNA-directed RNA polymerase (RdRP) system that can convert a small population of exog

126 RRF-1, a worm RNA-dependent RNA polymerase (RdRP) that is known to produce single-stranded secondary

127 1 is a putative RNA-directed RNA polymerase (RdRP) that is required for multiple aspects of C. elegan

128 more than one RNA-dependent RNA polymerase (RdRP) that probably emerged as a result of gene duplicat

129 irally encoded RNA-dependent RNA polymerase (RdRP) that uses a unique palm domain active site closure

130 y requires the RNA-dependent RNA polymerase (RdRP) to use 10-12 different mRNAs as templates for (-)

131 ly encode only RNA-dependent RNA polymerase (RdRP), capping enzyme and capsid protein.

132 depend on its RNA-dependent RNA polymerase (RdRP), composed of the PA, PB1, and PB2 subunits.

133 d by the viral RNA-dependent RNA polymerase (RdRP), consisting of the polymerase (L) protein complexe

134 to detect the RNA-dependent RNA polymerase (RdRP), envelope protein (E), and nucleocapsid protein (N

135 that SAD-1, an RNA-directed RNA polymerase (RdRP), is required for MSUD.

136 novo initiating RNA-directed RNA polymerase (RdRP), P2, forms the central machinery in the infection

137 recruitment of RNA-dependent RNA polymerase (RdRP), resulting in trans-generational gene silencing.

138 distinct viral RNA-directed RNA polymerase (RdRP), the hallmark gene of ribovirian kingdom Orthornav

139 ultifunctional RNA-dependent RNA polymerase (RdRP), which is composed of a large (L) protein that cat

140 a picornaviral RNA-dependent RNA polymerase (RdRP), which synthesizes double-stranded RNAs that are s

141 t depletion of RNA-dependent RNA polymerase (RdRP)-derived secondary small RNAs termed 22G-RNAs.

142 iated with the RNA-dependent RNA polymerase (RdRP).

143 and the RRF-3 RNA-dependent RNA polymerase (RdRP).

144 ute, dicer and RNA-dependent RNA polymerase (RdRP).

145 kinase and an RNA-dependent RNA polymerase (RdRP).

146 include viral RNA-dependent RNA polymerase (RdRP).

147 icivirus (FCV) RNA-dependent RNA polymerase (RdRP).

148 arget mRNAs by RNA-dependent RNA polymerase (RdRP).

149 virus-encoded RNA-dependent RNA polymerase (RdRP).

150 d by the viral RNA-dependent RNA polymerase (RdRP).

151 sis requires an RNA-directed RNA polymerase (RdRP)].

152 Dicer (Dcr1), RNA-dependent RNA polymerase (RdRP; Rdp1), and Argonaute (Ago1).

153 assay for the RNA-dependent RNA-polymerase (RdRP) of the arenavirus Machupo (MACV), we demonstrate t

154 d bound by the RNA-dependent RNA-polymerase (RdRP).

155 certain viral RNA-dependent RNA polymerases (RdRP) synthesizing RNA on RNA templates.

156 rally encoded RNA-dependent RNA polymerases (RdRP) with a common active-site structure and closure me

157 by the viral RNA-dependent RNA polymerases (RdRP).

158 e by cellular RNA-dependent RNA polymerases (RdRPs) and is required for the maturation of 26G-RNAs, D

159 Endogenous RNA-directed RNA polymerases (RdRPs) are cellular components capable of synthesizing n

160 Cellular RNA-dependent RNA polymerases (RdRPs) function in development and RNA-mediated silencin

161 Picornaviral RNA-dependent RNA polymerases (RdRPs) have low replication fidelity that is essential f

162 d the role of RNA-dependent RNA polymerases (RdRPs) in N. benthamiana antiviral defense.

163 The RNA-dependent RNA polymerases (RdRPs) of Cystoviridae bacteriophages, like those of euk

164 RNA-dependent RNA polymerases (RdRPs) of the Flaviviridae family catalyze replication o

165 ose essential RNA-dependent RNA polymerases (RdRPs) share a structurally homologous core with an enci

166 s of >330,000 RNA-dependent RNA polymerases (RdRPs) shows that this expansion corresponds to a 5-fold

167 l RNAs by two RNA-dependent RNA polymerases (RdRPs) that are thought to be tissue-specific - EGO-1 in

168 with distinct RNA-directed RNA polymerases (RdRPs) that seem to be intermediates between typical rib

169 des, cellular RNA-dependent RNA polymerases (RdRPs) use AGO targets as templates for amplification of

170 ng signals by RNA-dependent RNA polymerases (RdRPs).

171 tep involving RNA-dependent RNA polymerases (RdRPs).

172 es that act as RNA-directed RNA polymerases (RdRPs).

173 oth involving RNA-dependent RNA polymerases (RdRPs).

174 volutionary relationships based on predicted RdRP protein structures.

175 We also find that RDE-8 promotes RdRP activity, thereby ensuring amplification of siRNAs.

176 t multiple loci spanning the viral protease, RdRP, and capsid ORFs and isolated individual recombinan

177 We purified RdRPs using a recombinant influenza virus in which the P

178 By comparing the properties of RABV RdRP with those of the related rhabdovirus, vesicular st

179 e replaced by those of VSV P stimulated RABV RdRP activity on naked RNA but was insufficient to permi

180 The results show that the reconfigured RdRP mouse innate immune system substantially reduced re

181 Remarkably, RdRP mice never develop autoinflammation, interferonopat

182 o be intermediates between typical ribovirus RdRPs and viral reverse transcriptases.

183 ts for template recognition by the rotavirus RdRP and compared those to the requirements for formatio

184 his study maps a druggable target in the RSV RdRP and establishes clinical potential of the AVG chemo

185 iption and replication of the virus, the RSV RdRP is a logical target for novel antiviral drugs.

186 which targeting by ERGO-1 recruits a second RdRP (RRF-1 or EGO-1), which in turn transcribes 22G-RNA

187 nt antisense RNAs, dependent on a secondary RdRP (RRF-1) and associating with at least one distinct

188 Several RdRP chimeras supported the growth of infectious poliovi

189 Several RdRP-linked protein domains not previously detected in a

190 polymerases, implying that promoter-specific RdRP activation extends beyond the arenaviruses.

191 Such RdRP paralogs often participate in distinct RNA silencin

192 at cRNA synthesis requires newly synthesized RdRP and that incoming RdRP can only generate mRNA.

193 We also found that RdRP recognition signals are distinct from cis-acting si

194 The structures indicate that RdRPs use a fully prepositioned templating base for nucl

195 The RdRP (protein P2) is assembled within the procapsid, and

196 The RdRP is contained within a viral large (L) protein, whic

197 The RdRP RRF-1 colocalizes with MUT-16 at Mutator foci, sugg

198 We show that DCR-1, the RdRP RRF-3, and the dsRNA-binding protein RDE-4 are requ

199 by limited structural information about the RdRP catalytic cycle.

200 Although the RdRP alone can specifically bind to viral mRNAs, our ana

201 vent efficient recognition of the RNA by the RdRP.

202 Excluding the RdRP segment, we identified 106 unique viral genome segm

203 s the importance of these interfaces for the RdRP activity of the NSP7-NSP8-NSP12 complex.

204 mRNA fragments to serve as templates for the RdRP-directed amplification of the silencing signal.

205 e report the occurrence of a mutation in the RdRP (D484Y) following failure of remdesivir in a 76-yea

206 he roles of two CoV-specific residues in the RdRP active site: Ala547, which replaces a conserved glu

207 ependently evolved domain permutation in the RdRP.

208 ting plant defense response also induced the RdRP activity, whereas biologically inactive analogs did

209 During infection, the RdRP replicates and transcribes the viral genome, which

210 at ribavirin triphosphate (RTP) inhibits the RdRP.

211 und NP to allow for template access into the RdRP active site.

212 xes leading to a selective impairment of the RdRP activity.

213 ely studied, the underlying mechanism of the RdRP complex is still unclear.

214 To determine the initial location of the RdRP inside the procapsid of bacteriophage Phi6, we perf

215 luenza virus in which the PB2 subunit of the RdRP is fused to a Strep-tag.

216 structure proximal to the active site of the RdRP.

217 hance the initiation and processivity of the RdRP.

218 irectly or indirectly, with a subunit of the RdRP.

219 phorylated antisense RNAs that depend on the RdRP homolog RRF-3, the Argonaute ERGO-1, DICER, and a s

220 vely to form a unique functional site on the RdRP responsible for JAK-STAT inhibition.

221 ectron microscopy, we have reconstructed the RdRP tetramer complex at 4.3 A, highlighting the assembl

222 We further determined that the RdRP is expressed at low levels and that blocking Ifnar1

223 domain that is similar in appearance to the RdRP of dsRNA viruses and multiple accessory appendages

224 assays were deployed to the field, where the RdRP RT-RPA assays confirmed to produce the most accurat

225 ging from 24.9 to 36.8 nm, together with the RdRP protein, which was of an unexpected size.

226 735 (of 903), a range which lies within the RdRP domain.

227 stretches of specific amino acids within the RdRP, 374 to 380 and 624 to 647, as critical for inhibit

228 analysis of the amino acid sequences of the RdRPs from ScPV1 and related mycoviruses placed ScPV1 wi

229 However, phylogenetic analysis of the RdRPs supports the previously established five-branch st

230 palm, fingers, and thumb domains, and these RdRPs also possess a unique contact between the fingers

231 rmally limited in their accumulation by this RdRP.

232 To elucidate the antiviral role of this RdRP in a different host plant and to evaluate whether p

233 icance of genetic diversity acquired through RdRP, we characterize an IAV fidelity variant derived fr

234 A tobacco RdRP gene, NtRDRP1, was isolated and found to be induced

235 ecently found that the activity of a tobacco RdRP was increased in virus-infected or SA-treated plant

236 The requirement for two RdRP/Argonaute combinations and initiation by a rare cla

237 To isolate the function of the viral RdRP (NS5) from that of other host or viral factors pres

238 ions induced by the recruitment of the viral RdRP and host factors to subcellular membrane microdomai

239 The viral RdRP is an important host range determinant, indicating

240 y with the PB1 and PB2 subunits of the viral RdRP, and small interfering RNA (siRNA)-mediated knockdo

241 oration of ribonucleotides into RNA by viral RdRPs, thus providing important considerations for the d

242 RNA virus replication by plant viral RdRPs occurs inside vesicle-like membrane invaginations

243 ins that interact with the influenza A virus RdRP in infected human cells.

244 egulates the activity of the influenza virus RdRP.

245 ch other when modeled on the West Nile virus RdRP crystal structure.

246 imental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomi

247 ly conserved among positive-strand RNA virus RdRPs.

248 The in vitro RdRP assay system that utilizes cytoplasmic extracts fro

249 EN), a flavivirus family member, an in vitro RdRP assay was established using cytoplasmic extracts of

250 alen-UV cross-linking method and an in vitro RdRP assay, we analyzed structural determinants for phys

251 ssay is described here that utilizes the WNV RdRP and subgenomic (-)- and (+)-strand template RNAs co