ライフサイエンスコーパス: S-nitrosylated

1 ocysteine antibody indicated that PARP-1 was S-nitrosylated.

2 the zinc-tetrathiolate cysteines of eNOS are S-nitrosylated.

3 , the majority of mitochondrial caspase-9 is S-nitrosylated.

4 mulated to produce NO, the exogenous tTG was S-nitrosylated.

5 n, revealed that E1A and E7 are both protein-S-nitrosylated.

6 eines of TGFbeta1 which were predicted to be S-nitrosylated.

7 on of exogenous reagents (three of 21) or as S-nitrosylated.

8 complex formation was impeded when actin was S-nitrosylated.

9 Here, we show that PSD-95 is physiologically S-nitrosylated.

10 kinson's or Alzheimer's disease, that PDI is S-nitrosylated, a reaction transferring a nitric oxide (

11 Prior work has shown that hyperoxia causes S-nitrosylated actin (SNO-actin) formation, which mediat

12 mented polymerization normally observed with S-nitrosylated actin, VASP binding to actin, elevated Ra

13 , heterologously expressed human DDAH II was S-nitrosylated after cytokine induced expression of the

14 Thus, DR4 residue C336 becomes S nitrosylated and promotes apoptosis following NO-Cbl t

15 ne-permeable S-nitrosothiols and that sGC is S-nitrosylated and desensitized in the tolerant, treated

16 stasis, which were reflected by increases in S-nitrosylated and nitrated proteins in the lungs from i

17 of the HR, the bacterial effector HopAI1 is S-nitrosylated and that this modification inhibits its p

18 refore, we investigated whether Panx1 can be S-nitrosylated and whether this modification can affect

19 ), is progressively PKA-hyperphosphorylated, S-nitrosylated, and depleted of the phosphodiesterase PD

20 Here we show that cPLA(2)alpha protein is S-nitrosylated, and its activity is enhanced by nitric o

21 These findings suggest that S-nitrosylated arginase1 can compete with NOS for L-argi

22 t in GPCR trafficking, interacts with and is S-nitrosylated at a single cysteine by endothelial NO sy

23 ynamin, which interacts with NO synthase, is S-nitrosylated at a single cysteine residue (C607) after

24 or by iNOS induction caused GAPDH to become S-nitrosylated at Cys152.

25 As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay)

26 bc) release NO when hemoglobin that has been S-nitrosylated at Cys93 of the beta-chain (betaCys93) tr

27 ing cells, a subset of caspase-3 zymogens is S-nitrosylated at the active site cysteine, inhibiting e

28 lling H2O2 levels in plants, was found to be S-nitrosylated at the onset of both PCDs.

29 Low micromolar levels of S-nitrosylated bovine serum albumin (BSA), but not contr

30 Also, p50 was S-nitrosylated by DETANONOate resulting in inhibition of

31 udies also indicate that Ras Cys(118) can be S-nitrosylated by direct reaction of Cys(118) with a glu

32 ned from our studies suggest that Ras can be S-nitrosylated by direct reaction of Cys(118) with nitro

33 We report that NSF is physiologically S-nitrosylated by endogenous, neuronally derived nitric

34 A family of proteins S-nitrosylated by iNOS-S100A8/A9 were revealed by proteo

35 ase and sarcosine dehydrogenase (SarDH), are S-nitrosylated by NO under strictly anaerobic conditions

36 In both cells and tissues, GRK2 is S-nitrosylated by SNOs as well as by NO synthases, and G

37 Here we show that C. difficile toxins are S-nitrosylated by the infected host and that S-nitrosyla

38 Tissue TG can be poly-S-nitrosylated by the NO carrier, S-nitrosocysteine (Cys

39 Increased levels of S-nitrosylated C3 were present in female over male AD br

40 sult of the conformational change induced by S-nitrosylated CLIC4 that leads to unfolding of the prot

41 of PDZ-containing nNOS (nNOSalpha) increases S-nitrosylated CREB with consequent augmented binding on

42 Previous studies suggested that S-nitrosylated Cys may be flanked by an acid-base motif

43 ling proteomic strategy, we identified 1,276 S-nitrosylated cysteine residues [S-nitrosothiol (SNO)]

44 ed to ultraviolet light which photo-reverses S-nitrosylated cysteine residues and by co-incubations w

45 lls exposed to UV light, which photoreverses S-nitrosylated cysteine residues and by co-incubations w

46 Structural analyses revealed that S-nitrosylated cysteine residues were equally distribute

47 Seventy percent of the S-nitrosylated cysteine residues were surrounded by nega

48 Two potential S-nitrosylated cysteines in the alpha- and beta-subunits

49 , but unlike RyR1, RyR2 was not activated or S-nitrosylated directly by NO.

50 bl and subjected to the biotin switch assay; S-nitrosylated DR4 was detected in all three cell lines.

51 po2L/TRAIL death receptor DR4 (TRAIL R1) was S nitrosylated following NO-Cbl treatment.

52 in SP-D, including significant amounts of an S-nitrosylated form.

53 Specifically, we show that DJ-1 is S-nitrosylated (forming SNO-DJ-1); subsequently, the NO

54 Here we report that Prx2 is S-nitrosylated (forming SNO-Prx2) by reaction with nitri

55 the BST using differentially S-oxidized and S-nitrosylated forms of protein tyrosine phosphatase 1B,

56 This is mediated by S-nitrosylated-GAPDH, which simultaneously inactivates S

57 A 2-fold increase in S-nitrosylated GCK was also observed in mouse islets.

58 atalyzing TrxR1-dependent denitrosylation of S-nitrosylated glutathione or of HEK293 cell-derived S-n

59 S-nitrosylated hemoglobin (SNO-Hb) within red blood cell

60 athway for the formation of beta-93 cysteine S-nitrosylated hemoglobin [SNOHb:S-nitrosohemoglobin], w

61 study reveal a previously unexplored role of S-nitrosylated hemoglobin in cardioprotection.

62 tivities and in assays of endogenous Fe- and S-nitrosylated hemoglobin.

63 We further show that S-nitrosylated HIF-1alpha binds to the vascular endothel

64 In contexts where pp71 is not protein S-nitrosylated, host antiviral response was inhibited.

65 is evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, sugg

66 ed that protein disulfide isomerase (PDI) is S-nitrosylated in brains of patients with sporadic neuro

67 ecently reported that eNOS is constitutively S-nitrosylated in endothelial cells and that agonists pr

68 s involved in contraction and relaxation are S-nitrosylated in laboring and nonlaboring muscle and th

69 aute protein (ALG-1)-at a site conserved and S-nitrosylated in mammalian Argonaute 2 (AGO2)-alters it

70 and that many of these proteins are uniquely S-nitrosylated in only one state of the tissue.

71 We now show that sGC can be S-nitrosylated in primary aortic smooth muscle cells by

72 h inducible nitric oxide synthase (iNOS) and S-nitrosylated in proinflammatory peritoneal macrophages

73 We report that eNOS is tonically S-nitrosylated in resting bovine aortic endothelial cell

74 ve recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury e

75 dynamic cycle exists in which haemoglobin is S-nitrosylated in the lung when red blood cells are oxyg

76 Remarkably, SRG3 but not SRG2 was S-nitrosylated in vitro and in vivo.

77 We show that parkin is S-nitrosylated in vitro, as well as in vivo in a mouse m

78 contains approximately 84 free thiols and is S-nitrosylated in vivo.

79 Furthermore, we identified that gephyrin is S-nitrosylated in vivo.

80 In addition, treatment with VEGF-A decreased S-nitrosylated laminin in cultured podocytes.

81 We show that SR is physiologically S-nitrosylated leading to marked inhibition of enzyme ac

82 , we report that E1A and E7 are both protein-S-nitrosylated like pp71.

83 o systematically investigate oxidized and/or S-nitrosylated mitochondrial proteins and to use a bioti

84 d markedly increased numbers of oxidized and S-nitrosylated mitochondrial proteins following hepatic

85 The oxidized and/or S-nitrosylated mitochondrial proteins from I/R-injured m

86 e biotin-N-maleimide-labeled oxidized and/or S-nitrosylated mitochondrial proteins from pair-fed cont

87 easing synthesis of nitric oxide (NO), which S-nitrosylated N-ethylmaleimide sensitive factor (NSF),

88 NO increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels decrease within 3 h after expo

89 Endogenously synthesized NO increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels

90 Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosi

91 and NSF, and endogenous TRX1 removes NO from S-nitrosylated NSF.

92 Kdm1a, a well-known epigenetic modifier, is S-nitrosylated on Cys334.

93 ondition, the class I member HDAC2 was found S-nitrosylated on cysteine, a post-transduction modifica

94 Furthermore, we show that BVR is S-nitrosylated on one of three cysteines and that this p

95 Caspase-3 zymogens were found to be S-nitrosylated on their catalytic-site cysteine in unsti

96 Finally S-nitrosylated ornithine decarboxylase was isolated from

97 or was protein kinase A-hyperphosphorylated, S-nitrosylated, oxidized, and depleted of its stabilizin

98 Further, an increase in S-nitrosylated p50 was detected in cells, and the level

99 nt gene transcription, and nuclear levels of S-nitrosylated p65 correlate with decreased DNA binding

100 We demonstrate that COP1 is S-nitrosylated, particularly under shade.

101 steinyl affinity resin to selectively enrich S-nitrosylated peptides reduced by ascorbate followed by

102 Upon M.tb infection, secreted S-nitrosylated PknG phosphorylates macrophage Silencer o

103 A subset of proteins are constitutively S-nitrosylated, playing roles in the regulation of tissu

104 ce that inhibition of the denitrosylation of S-nitrosylated procaspase-3 mediated by the redox protei

105 Levels of S-nitrosylated protein are controlled in part by the den

106 ase, eliminated the light-evoked increase in S-nitrosylated protein immunofluorescence (SNI) in the r

107 2+ channel alpha1 subunit as the predominant S-nitrosylated protein in membrane fractions, and follow

108 ury and improved survival rates by restoring S-nitrosylated protein levels in the brain after CA/CPR

109 art, liver, spleen, and plasma, and restored S-nitrosylated protein levels in the brain.

110 MMP activity colocalized with immunoreactive S-nitrosylated protein.

111 Here, we discuss how aberrantly S-nitrosylated proteins (SNO-proteins) play a crucial ro

112 Steady-state levels of S-nitrosylated proteins are largely determined by denitr

113 placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxida

114 We identified several candidate S-nitrosylated proteins by proteomic analysis following

115 h S-nitrosoglutathione or denitrosylation of S-nitrosylated proteins by reduced GSH.

116 n increase of up to 11-fold in the number of S-nitrosylated proteins during regeneration.

117 ective of the type of S-nitrosylating agent, S-nitrosylated proteins formed upon exposure to both of

118 Only a few intracellular S-nitrosylated proteins have been identified, and it is

119 2809 SNO sites, representing a wide range of S-nitrosylated proteins in 40 postmortem AD and non-AD h

120 Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chem

121 We identify endogenously S-nitrosylated proteins in subcellular organelles, inclu

122 ion prevented the reduction in the number of S-nitrosylated proteins in the brain, mitigated brain in

123 PR activated GSNOR and reduced the number of S-nitrosylated proteins in the brain.

124 An unbiased proteomic approach to identify S-nitrosylated proteins in valve cells found enrichment

125 Functional analysis of differentially S-nitrosylated proteins indicated their involvement in a

126 Determination of S-nitrosylated proteins is of great importance for funda

127 -RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitate

128 The overall patterns of oxidized and/or S-nitrosylated proteins resolved by 2-dimensional polyac

129 gical outcome, GSNOR activity, and levels of S-nitrosylated proteins were evaluated.

130 In addition, S-nitrosylated proteins were quantitated using the fluor

131 proteomic identification of over a thousand S-nitrosylated proteins, few S-nitrosylases have been id

132 ntrols intracellular levels of both GSNO and S-nitrosylated proteins.

133 groups, including many previously unrevealed S-nitrosylated proteins.

134 a modified biotin switch method to identify S-nitrosylated proteins.

135 s associated with alterations in the nuclear S-nitrosylated proteome.

136 Accordingly, by decreasing S-nitrosylated Prx2 (SNO-Prx2), overexpression of Srxn1

137 S-Nitrosylated Ras (Ras-SNO) can be formed when NO serve

138 pathways affected by iNOS indicated that NO S-nitrosylated Ras.

139 dithiothreitol, which triggers reduction of S-nitrosylated residues.

140 ry neurons that stargazin is physiologically S-nitrosylated, resulting in increased surface expressio

141 he RyR1 macromolecular complex was oxidized, S-nitrosylated, Ser-2844 phosphorylated, and depleted of

142 ed protein (VASP) exhibits high affinity for S-nitrosylated short filamentous actin, which increases

143 e SHP-2 at its active site cysteine, forming S-nitrosylated SHP-2 (SNO-SHP-2).

144 litates identification and quantification of S-nitrosylated sites by mass spectrometry.

145 These are 408 S-nitrosylated sites in 360 protein groups showing an ac

146 the ease with which it can detect individual S-nitrosylated (SNO) proteins in biological samples.

147 By native PAGE, formation of S-nitrosylated SP-D in vivo resulted in disruption of SP

148 y recruitment of effector cells modulated by S-nitrosylated SP-D.

149 with the Cys(85) thiol group in reduced and S-nitrosylated states.

150 The cellular content of S-nitrosylated Syntaxin 4 peaked acutely, within 5 min o

151 l treatments, including well known and novel S-nitrosylated targets.

152 , whereas specific, high affinity binding of S-nitrosylated TG2 (SNO-TG2) to endothelial surfaces res

153 a set of mitochondrial proteins that can be S-nitrosylated through multiple reaction channels, inclu

154 ion suggested that multiple cysteines may be S-nitrosylated to regulate Panx1 channel function.

155 This was associated with the appearance of S-nitrosylated TonEBP/NFAT5, as monitored by the biotin-

156 Furthermore, S-nitrosylated tTG inhibited platelet aggregation induce

157 ry revealed more than 300 proteins that were S-nitrosylated upon illumination, many of which are know

158 Furthermore, S-nitrosylated USP9X was shown to deubiquitinate and sta

159 es that encode a pp65 that cannot be protein-S-nitrosylated, we evaluated the impact of this PTM on v

160 While wild-type eNOS is robustly S-nitrosylated, we found that S-nitrosylation of the Myr

161 amino acids we identified in pp71 as protein S-nitrosylated, we found this pp71 PTM diminishes its ab

162 To identify which cysteine residue(s) was S-nitrosylated, we made single cysteine-to-alanine subst

163 ive site Cys(453) determined by isolation of S-nitrosylated wild type but not active site Cys(453) --