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1 S. pneumoniae (98/458; 21.4%) and Mycobacterium tubercul
2 S. pneumoniae density was substantially higher in vaccin
3 S. pneumoniae established effective commensal colonizati
4 S. pneumoniae invades the myocardium and induces cardiac
5 S. pneumoniae isolates were serotyped and tested for ant
6 S. pneumoniae responds to exogenous fatty acids by suppr
7 S. pneumoniae strain JMG1 (DeltafakB3) was deficient in
8 S. pneumoniae that colonized the respiratory epithelium
9 S. pneumoniae was detected in the myocardium of all NHPs
10 S. pneumoniae was the most common bacterial pathogen, ac
11 S. pneumoniae was the most common pathogen detected (n =
12 S. pneumoniae-induced suppression of the P2Y(2)-mediated
13 S. pneumoniae-PR8 coinfection elicited a robust IL-17A r
14 ia coli isolates by MIC and 30 S. aureus, 15 S. pneumoniae, and 15 S. pyogenes isolates by disk diffu
16 uency and other associated phenotypes of 208 S. pneumoniae clinical isolates representing at least 30
19 value identification identified correctly 46 S. pneumoniae and 4 S. pseudopneumoniae but misidentifie
20 onfirmed as N. meningitidis (n = 2433; 56%), S. pneumoniae (n = 1758; 40%), or H. influenzae (n = 180
21 istant sequence type 156 (ST156) serotype 9V S. pneumoniae in 3 respiratory patients that resulted in
22 In addition, the UAD-2 assay identified a S. pneumoniae serotype in 3.72% of nonbacteremic CAP cas
24 hibited their antibacterial activity against S. pneumoniae but did not affect their ability to activa
25 all displayed bactericidal activity against S. pneumoniae, but only CCL26 and CCL28 retained high an
28 as the host response to AA protected against S. pneumoniae lung disease, the IL-6 deficiency abrogate
30 efore a promising drug lead scaffold against S. pneumoniae infections that could be administered indi
32 spectra evaluation correctly identified all S. pneumoniae and S. pseudopneumoniae strains but miside
34 red by PAF and the bacterial cell wall allow S. pneumoniae to leverage a ChoP-remodeling enzyme (Pce)
35 d to the virulence of S. pneumoniae Although S. pneumoniae is known to use a sophisticated enzyme mac
37 studied histidine triad protein D (PhtD), an S. pneumoniae adhesin vaccine candidate, for its ability
38 del of secondary bacterial pneumonia with an S. pneumoniae strain that is innocuous to mice in the ab
39 Specific qPCR tests targeting S. aureus and S. pneumoniae did not provide additional diagnoses but p
41 mice from septicemia caused by S. aureus and S. pneumoniae, whereas untreated mice die within 24-33 h
45 ainst nontypeable Haemophilus influenzae and S. pneumoniae, engendering protection against acute otit
50 % CI = 1.29-4.88; n = 921 participants), and S. pneumoniae community-acquired pneumonia (OR = 2.15; 9
51 neuraminidase-expressing influenza virus and S. pneumoniae potentiates both colonization and infectio
53 tic fluoroquinolone-resistant MRSA, VRE, and S. pneumoniae, and the possibility to offer patients an
58 or the immunochromatographic (ICT) BinaxNow S. pneumoniae test (composite diagnostic) was positive.
59 acological inhibition of this enzyme blocked S. pneumoniae-induced PMN transepithelial migration in v
60 intercepting the competence regulon in both S. pneumoniae specificity groups with activities at the
62 2013-2015, and 2016-2018 in cases caused by S. pneumoniae (5.1% [15/294], 65.9% [58/88], and 52.1% [
64 xacerbates nasal colonization and disease by S. pneumoniae, in part via the synergistic contributions
66 ssion and middle ear inflammation induced by S. pneumoniae and reduced hearing loss and pneumococcal
67 thophysiology of oxidative stress induced by S. pneumoniae and the role of nuclear factor erythroid 2
69 with acute pneumonia, and H2O2 production by S. pneumoniae in vivo contributes to its genotoxicity an
70 tibodies against host proteins recognized by S. pneumoniae adhesins, we showed that S. pneumoniae upt
71 geal colonisation model, black carbon caused S. pneumoniae to spread from the nasopharynx to the lung
72 tion, and influenza virus coinfection caused S. pneumoniae NP density to increase, resulting in bacte
73 absence of Pce, neutrophils rapidly cleared S. pneumoniae from the airway and impeded invasive disea
74 The TAC method was evaluated on 146 clinical S. pneumoniae isolates and 13 nonpneumococcal species th
75 such as influenza A virus, induces commensal S. pneumoniae to disseminate beyond the nasopharynx and
77 utrophils were indispensable for controlling S. pneumoniae outgrowth but contributed to alveolar barr
79 -specific IgGs in serum, the newly developed S. pneumoniae CPS microarrays offer the advantage of ena
81 arbon sources on CPS production in different S. pneumoniae serotypes may contribute to a better under
83 -off absorbance-value of 2.1, differentiated S. pneumoniae from all but one other mitis group strepto
84 uring both colonization and invasive disease S. pneumoniae ferments host-derived carbohydrates as its
86 data demonstrate a key role for PAR-1 during S. pneumoniae lung infection that is mediated, at least
87 , we show that Il22ra2 inhibits IL-22 during S. pneumoniae lung infection and that Il22ra2 deficiency
89 As (miRs) in lung neutrophils in mice during S. pneumoniae pneumonia and performed in depth in silico
90 on with a high-dose inoculum of encapsulated S. pneumoniae, alveolar macrophage-independent clearance
94 injured mice had greater mortality following S. pneumoniae infection, which suggests that respiratory
95 with pneumonia who had positive cultures for S. pneumoniae from January 1, 2000 to December 31, 2013.
98 d here confirm the importance of pilus I for S. pneumoniae pathogenesis and the potential use of anti
100 aecalis ATCC 29212, 0.008 to 0.03 mug/ml for S. pneumoniae ATCC 49619, and 2 to 8 mug/ml for H. influ
101 mm for S. aureus ATCC 25923, 25 to 31 mm for S. pneumoniae ATCC 49619, and 16 to 20 mm for H. influen
102 oncentration (MIC) of RSM-932A and MN58b for S. pneumoniae was 0.4 muM and 10 muM, respectively, and
103 ): of 35 samples that were qPCR positive for S. pneumoniae, N. meningitidis, and H. influenzae, only
105 in (91% sensitivity and 100% specificity for S. pneumoniae and 81% sensitivity and 100% specificity f
106 on (75% sensitivity and 100% specificity for S. pneumoniae) for clinical metagenomic sputum samples.
107 es and also with potassium and thymidine for S. pneumoniae For all other variations, gepotidacin MIC
108 and coculture of these respective APCs from S. pneumoniae- or OVA-immunized mice with OVA-specific T
111 drate-binding module (CBM), originating from S. pneumoniae, with a synthetic B type 2 neoglycolipid,
112 ultiple-antigen vaccines (MAV) prepared from S. pneumoniae TIGR4 lysates enriched for surface protein
113 vide immediate and essential protection from S. pneumoniae through production of natural Ig, which ha
114 nge of H(2)O(2) concentrations in vitro from S. pneumoniae, highlighting their potential for facile r
116 ern because - in contrast to HRSV and HMPV - S. pneumoniae can become part of the nasopharyngeal flor
117 enotypic TaqMan array card (TAC) to identify S. pneumoniae strains, including lytA-based sequences, a
118 he A- and B-site SczA mutant variants impact S. pneumoniae resistance to zinc toxicity and survival i
120 tial role for neutrophil-derived IL-1beta in S. pneumoniae infection, and they elucidate the role of
122 the mgtA gene encoding a PII-type ATPase in S. pneumoniae, suggested previously to function in Ca2+
124 reverse-engineer gene expression control in S. pneumoniae A selection platform is described that all
133 is shown here through functional studies in S. pneumoniae that an unannotated homodimeric TetR from
134 ster than observed over longer timescales in S. pneumoniae and other bacteria drives high within-host
136 e against extracellular pathogens, including S. pneumoniae, we hypothesized that ethanol impairs muco
138 ivative of the alkaloid vincamine, inhibited S. pneumoniae-induced mucin MUC5AC upregulation in cultu
140 derived cells, depleted by CLs, internalized S. pneumoniae in vivo, whereas CD11c(low) dendritic cell
144 utcome with regard to prevention of invasive S. pneumoniae pathogenesis with a protein vaccine simila
146 rrow neutrophils stimulated with heat-killed S. pneumoniae (signal 1) and pneumolysin (signal 2) exhi
147 s of IP children stimulated with heat-killed S. pneumoniae had significantly reduced percentages of C
148 dependent cytolysin (CDC) family, is a major S. pneumoniae virulence factor that generates ~25-nm dia
150 erage annual incidences for N. meningitidis, S. pneumoniae, and H. influenzae, respectively, were 7.5
151 e presence of interesting antibacterial [MIC(S. pneumoniae) approximately 1.2 muM] and anticancer [IC
152 actiae, S. dysgalactiae, S. equi, S. mutans, S. pneumoniae, S. suis and S. uberis, as well as represe
154 erstanding the epidemiology of nonbacteremic S. pneumoniae CAP and for assessing the efficacy of futu
162 nother tool that is unique in the arsenal of S. pneumoniae and that it may implement the effort of th
163 n stress, but only in genetic backgrounds of S. pneumoniae and Bacillus subtilis that exhibit Mn2+ se
164 cifically affecting the adhesive capacity of S. pneumoniae led to the identification of the monoclona
165 r understanding of the extensive capacity of S. pneumoniae to process host glycans and the likely rol
168 hat CozE is a member of the MreCD complex of S. pneumoniae that directs the activity of PBP1a to the
170 mples for the serotype-specific detection of S. pneumoniae Sputum optimization involved liquefaction
171 at qPCR significantly increases detection of S. pneumoniae, N. meningitidis, and H. influenzae in CSF
172 , direct, and serotype-specific detection of S. pneumoniae, which may improve postvaccination serotyp
175 th cases and controls, with the exception of S. pneumoniae in exposed controls, which was detected 25
176 molysin (PLY) is a major virulence factor of S. pneumoniae and a target for both small molecule drug
180 -induced oxidative stress was independent of S. pneumoniae-derived H2O2 and pneumolysin but depended
184 d whole-genome sequencing of 140 isolates of S. pneumoniae recovered from bloodstream infection (n =
188 tudy, we used a model of low multiplicity of S. pneumoniae infection with HL-1 mouse cardiomyocytes t
189 necroptosis inhibition reduced the number of S. pneumoniae foci observed in hearts of acutely infecte
193 V also significantly reduced phagocytosis of S. pneumoniae by 33% in COPD MDM (n = 20; P = 0.0192).
195 ed response is suppressed in the presence of S. pneumoniae in A549 and isolated primary alveolar cell
196 pre-PCV and post-PCV eras, the prevalence of S. pneumoniae bacteremia dropped across all age groups (
197 as observed with regard to the prevention of S. pneumoniae bacteremia, and there was no difference in
199 n patients with pneumonia, the proportion of S. pneumoniae-specific plasmablasts expressing L-selecti
200 ion with PCV13 led to a greater reduction of S. pneumoniae NP density (>2.5 log units) than PhtD vacc
201 s, attesting to intracellular replication of S. pneumoniae as a key first step in pneumococcal pathog
202 an hydrolase CbpD that targets the septum of S. pneumoniae cells to show that class A PBPs have an au
203 ressure by human CMV and the 23F serotype of S. pneumoniae acted on the IGVK3-11 and IGVH3-30 genes a
204 o both an invasive and noninvasive strain of S. pneumoniae (D39 and EF3030) but that PAR-1 antagonism
205 , we compared an isogenic deletion strain of S. pneumoniae TIGR4 in polyamine transport operon (Delta
207 wofold higher expression compared to that of S. pneumoniae R6, could also confer increased resistance
209 y that is tightly linked to the virulence of S. pneumoniae Although S. pneumoniae is known to use a s
210 tic activity, colonization, and virulence of S. pneumoniae, as well as host cell myeloperoxidase acti
212 opsonic capacity by increasing C3 binding on S. pneumoniae Taken together, endogenous IL-22 and hepat
213 n, and cutaneous lymphocyte antigen (CLA) on S. pneumoniae-specific plasmablasts was examined in pati
215 lide use and PCV7 and PCV13 introductions on S. pneumoniae were associated with changes in macrolide
217 t in one of the CBPs, demonstrated that only S. pneumoniae lacking the CBP pneumococcal surface prote
218 dens upon superinfection with either MRSA or S. pneumoniae Surprisingly, adhesion molecule expression
219 s after intratracheal instillation of PBS or S. pneumoniae, and differentially expressed (DE) mRNAs a
221 (N. meningitidis), Streptococcus pneumoniae (S. pneumoniae), and Haemophilus influenzae type b (Hib)
222 eus (S. aureus) or Streptococcus pneumoniae (S. pneumoniae), respectively; and a qPCR assay targeting
223 with positive UAT more often had a positive S. pneumoniae culture (25.4% vs. 1.9%, p<0.001) and less
224 uenza virus on the surface of Gram-positive, S. pneumoniae and S. aureus, and Gram-negative, Moraxell
230 Our data show that although IL-17A reduces S. pneumoniae colonization, coinfection with influenza v
231 atients hospitalized for macrolide-resistant S. pneumoniae pneumonia were more severely ill on presen
233 e regulon can therefore be utilized to study S. pneumoniae cell-cell communication and behavioral cha
234 populations and MZ B cells regulate systemic S. pneumoniae clearance through complementary mechanisms
236 of evolutionary theory, we hypothesized that S. pneumoniae use owner-intruder asymmetries to settle c
237 contractility; (2) the new observation that S. pneumoniae is capable of translocation into the myoca
239 orescence microscopy (IFM), we observed that S. pneumoniae replication within the heart preceded visu
240 pinning-disk live-cell imaging revealed that S. pneumoniae induces P2Y(2) translocation into the cyto
241 umococcal carriage model (EHPC) to show that S. pneumoniae colonisation is associated with epithelial
244 smission electron microscopy, we showed that S. pneumoniae rapidly adhered to and invaded cardiomyocy
245 ed by S. pneumoniae adhesins, we showed that S. pneumoniae uptake by cardiomyocytes is not through th
248 al doubling time increased to 56 min and the S. pneumoniae alveolar macrophage-dependent clearance ha
249 We first described a UAD assay to detect the S. pneumoniae serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14,
250 r haptenic component of teichoic acid in the S. pneumoniae cell wall, and lipoteichoic acid in the S.
251 niae cell wall, and lipoteichoic acid in the S. pneumoniae membrane were previously reported to be im
252 eptococcus pneumoniae Some components of the S. pneumoniae glycoconjugate vaccine Prevnar13 that cont
255 In addition, our results suggest that the S. pneumoniae yybP-ykoY riboswitch functions to regulate
258 -binding residues, PspCNs from D39 and Tigr4 S. pneumoniae exhibit similar FH-anchoring and enhancing
259 pulmonary neutrophils, a level comparable to S. pneumoniae-challenged, conventionally fed young mice.
260 ht on the susceptibility of older persons to S. pneumoniae and provide a possible therapeutic to impr
261 he acute-phase protein C-reactive protein to S. pneumoniae, thereby reducing activation of the classi
263 the age-associated decline in resistance to S. pneumoniae, young (4 mo) and old (22-24 mo) C57BL/6 m
264 Il22ra2(-/-) mice are more resistant to S. pneumoniae infection, have increased IL-22 in lung ti
270 ver, Stat1(-/-) mice are more susceptible to S. pneumoniae infection, which can be rescued by the ser
272 cus pneumoniae [including the unencapsulated S. pneumoniae, serotype 2 strain (R36A)] markedly inhibi
273 form of PLA2 (cPLA2alpha) was activated upon S. pneumoniae infection of cultured lung epithelial cell
274 ive immune response against native CPS using S. pneumoniae serotype 5 (ST-5), a problematic CPS compo
275 of within-host competitive success utilizing S. pneumoniae colonization of the upper respiratory trac
277 oimmunization studies using cOVA and various S. pneumoniae mutants, each genetically deficient in one
283 systemic infection after lung challenge with S. pneumoniae As phospholipase A2 (PLA2) promotes the re
284 mice upon high-dose pulmonary challenge with S. pneumoniae The cPLA2alpha-deficient mice also suffere
286 logical consolidation or pleural fluid, with S. pneumoniae and S. aureus the leading pathogens identi
287 (+) T cells in response to immunization with S. pneumoniae expressing OVA peptide, did not inhibit T
288 6 (control) mice intravenously infected with S. pneumoniae were treated intravenously with PFCE or ph
289 In experiments with SCID mice infected with S. pneumoniae, we found passive transfer of IgG-depleted
293 he protection of mice against infection with S. pneumoniae in which iNKT cells have previously been f
297 7BL/6 mice were intranasally inoculated with S. pneumoniae serotype 6A to establish colonization and
298 rtical impact and infected intranasally with S. pneumoniae (1,500 colony-forming units) or vehicle (p
300 colonized the NPs of adult C57BL/6 mice with S. pneumoniae serotype (ST) 6A or 8 and then coinfected