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1 S. pyogenes bound purified fibulin-1 in a dose-dependent
2 S. pyogenes expresses HSA-binding surface proteins, and
3 S. pyogenes grew from one or both swabs for 198 (37%) of
4 S. pyogenes MHC class II-bound peptide-specific CD4(+) T
5 S. pyogenes NCTC 8198(T) and CCUG 4207(T) are derived fr
6 S. pyogenes strains with this type of polymorphism cause
7 S. pyogenes-induced Th17 formation depended on TGF-beta1
10 In all, 1,800 Staphylococcus aureus, 259 S. pyogenes, 226 Streptococcus pneumoniae, 93 Enterococc
11 2 opsonized SOF-positive M type 2, 4, and 28 S. pyogenes in human blood but had no effect on SOF-nega
15 rated in erythromycin-resistant M/emm type 6 S. pyogenes, which raises concern for the emergence of m
16 ysis shows that in contrast to the type II-A S. pyogenes Cas9 that is widely used for genome engineer
20 gm shift from type-specific immunity against S. pyogenes to emm-cluster based immunity for this bacte
26 examine this, we analyzed the behavior of an S. pyogenes mutant deficient in expression of the cytoly
27 nally, an allelic replacement analysis of an S. pyogenes strain with a naturally occurring insertion
28 ant to in vitro penetration by S. aureus and S. pyogenes and partially resistant to P. aeruginosa.
29 also prevented penetration by S. aureus and S. pyogenes; NeoForm was less effective in withstanding
31 tween S. dysgalactiae subsp. equisimilis and S. pyogenes alleles revealed a history of interspecies r
35 he surface of Gram-positive bacteria such as S. pyogenes will enable professional phagocytes to elimi
36 y can be applied for specific agents such as S. pyogenes, or commercial multiplex NAATs for detection
41 ology, and public health communities because S. pyogenes has remained universally susceptible to beta
43 c toxin B (SPE B) on the interaction between S. pyogenes strain NZ131 (serotype M49) and mammalian ce
44 en grown in sugar-limited Todd-Hewitt broth, S. pyogenes cells remained culturable for more than 1 ye
46 ds, the induction of caspase-1 activation by S. pyogenes did not require exogenous ATP or the P2X7R.
55 that fluoride causes decreased expression by S. pyogenes proteins used to respond to stress, virulenc
58 cells prevented nasopharyngeal infection by S. pyogenes, but not by Streptococcus pneumoniae, a bact
59 y-phase culture supernatant proteins made by S. pyogenes NZ131 rgg and NZ131 speB were separated by t
61 Binding of complement regulatory proteins by S. pyogenes has previously been attributed to the strept
62 ed proteomic analysis of protein released by S. pyogenes into the culture supernatant and observed de
63 , and NaCl may mimic relevant cues sensed by S. pyogenes during infection; and that identification of
69 udy, we quantitatively analyzed and compared S. pyogenes proteins in the growth medium of a strain th
70 sing bioinformatics analysis of the complete S. pyogenes strain SF370 genome, we have identified a no
71 gest that under certain in vitro conditions, S. pyogenes cells can persist for greater than 1 year as
74 ensors were also able to specifically detect S. pyogenes in 50% (v/v) human saliva, with good selecti
75 trations of saliva and plasma, and different S. pyogenes serotypes and their isogenic mutants, reveal
79 ome sequences of type emm1, emm28, and emm89 S. pyogenes clinical strains recovered from intercontine
80 we successfully delivered a plasmid encoding S. pyogenes Cas9 and sgRNA to the corneal epithelium by
83 val niche and conduit to the bloodstream for S. pyogenes, explaining the phenomenon of occult bactera
84 e clinical settings, by a throat culture for S. pyogenes to increase the sensitivity of its detection
85 roxidase in S. pyogenes and is essential for S. pyogenes pathogenesis in several murine models that m
87 based system as a standard typing scheme for S. pyogenes will facilitate the design of future studies
88 often highly related, but they differed from S. pyogenes, in that S. dysgalactiae subsp. equisimilis
89 strate that the multidomain protein Epf from S. pyogenes serotype M49 is a streptococcal adhesin.
90 nce analyses of larger regions of FnBPs from S. pyogenes and S. aureus reveal a repeating pattern of
91 his is the first methyltransferase gene from S. pyogenes to be cloned and to have its activity charac
92 o compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to pro
93 sphoribosyltransferase activity of NadC from S. pyogenes allows the organism to sustain growth when Q
94 monstrated that the released M1 protein from S. pyogenes activates platelets, and this activation is
95 from those recognized by Cas9 proteins from S. pyogenes and S. thermophilus (SpCas9 and StCas9, resp
96 rated cellular and supernatant proteins from S. pyogenes cultures by high-resolution two-dimensional
97 28-kDa streptococcal protease purified from S. pyogenes processed the 40-kDa mutant zymogen to a 28-
98 ial pathogen the group A Streptococcus (GAS; S. pyogenes) as a model organism, we review the types an
99 and virulence in group A Streptococcus (GAS; S. pyogenes), the precise role of the co-transcribing se
102 an insertionally inactivated degP allele in S. pyogenes is similar to that reported for E. coli, wit
105 sion, the elucidation of GAC biosynthesis in S. pyogenes reported here enhances our understanding of
108 Taken together, the CvfA-enolase complex in S. pyogenes is involved in the regulation of virulence g
109 s aureus and Listeria monocytogenes, DacA in S. pyogenes was not essential for growth in rich media.
113 pA, the gene which encodes trigger factor in S. pyogenes, produced mutant proteins deficient in PPIas
116 he first hemoprotein receptors identified in S. pyogenes; their possible role in iron capture is disc
121 idase is the major glutathione peroxidase in S. pyogenes and is essential for S. pyogenes pathogenesi
122 acterized an intergenic VNTR polymorphism in S. pyogenes that affects toxin production and virulence.
127 rify the function of HSA-binding proteins in S. pyogenes and underline the power of the quantitative
129 ion of NadD confirmed its functional role in S. pyogenes, and its potential as an antibacterial targe
131 or induced resistance to peroxide stress in S. pyogenes, genes for a novel mechanism of managing per
133 oper response to stress and for virulence in S. pyogenes, suggesting that its signaling pathway could
134 Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-p
135 m by which Gram-positive bacteria, including S. pyogenes, coordinate multiple environmental cues, all
136 , and some Gram-positive pathogens including S. pyogenes use this cyclic nucleotide derivative as a s
137 gainst most streptococcal species, including S. pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S.
138 hat M. catarrhalis can dramatically increase S. pyogenes adherence to human epithelial cells and that
139 ne of these inhibitors efficiently inhibited S. pyogenes NadD (sp.NadD) in vitro (50% inhibitory conc
140 signaling protects the host against invasive S. pyogenes infection by restricting inflammation-driven
142 pooled human immunoglobulin during invasive S. pyogenes infection, and demonstrate a potential route
144 ernatants prepared from cultures of invasive S. pyogenes strains of varying serotypes in the stationa
145 and the finding that patients with invasive S. pyogenes infection respond with antibody production a
146 ysis of samples from a patient with invasive S. pyogenes infection revealed dramatic differences in t
147 The utility of our model for investigating S. pyogenes factors contributing to mucosal carriage was
149 retion were induced by live, but not killed, S. pyogenes, and required expression of the pore-forming
150 tudy it is shown that AspA from serotype M28 S. pyogenes, when expressed on surrogate host Lactococcu
152 SPN and SLO in epidemic serotype M1 and M89 S. pyogenes strains is associated with rapid intercontin
153 0% S), and MIC(90)s ranged from 0.03 mug/ml (S. pyogenes/S. pneumoniae) to 1 mug/ml (Enterobacteriace
156 ht of reports of hypervirulent SpeB-negative S. pyogenes variants present during invasive infections.
158 In conclusion, we have identified a novel S. pyogenes enzyme with 5'-nucleotidase activity and imm
159 ain SF370 genome, we have identified a novel S. pyogenes virulence factor, which we termed streptococ
161 mbinant SOF from M types 2, 4, 28, and 75 of S. pyogenes, indicating that the fibulin-1-binding domai
162 nsporter may be an example of the ability of S. pyogenes to adapt and evolve new survival strategies
163 of zinc homeostasis inhibits the ability of S. pyogenes to cause disease in a zinc-limited host mili
164 l regulatory system governing the ability of S. pyogenes to colonize the nasopharynx and provides kno
165 s rescued, demonstrating that the ability of S. pyogenes to utilize arginine was dispensable in the a
166 in the absence of FBP, Pi is an activator of S. pyogenes LDH, E. faecalis LDH1, and L. lactis LDH1 an
167 ered metabolism, the catabolic activities of S. pyogenes strain NZ131 (serotype M49) and an isogenic
172 factors for the survival and colonization of S. pyogenes is well established, and many of these facto
173 plicated in non-suppurative complications of S. pyogenes, including glomerulonephritis and rheumatic
176 ity factor (SOF), a virulence determinant of S. pyogenes, reduced binding by approximately 50%, and a
178 the role of prophages in diversification of S. pyogenes and the close relationship between strain Ma
184 ow the intracellular proteome homeostasis of S. pyogenes is influenced by the presence of human plasm
186 This study was a unique investigation of S. pyogenes factors required for successful invasive inf
187 vival of clinical and laboratory isolates of S. pyogenes and S. pneumoniae as both organisms are thou
188 ng system conserved in sequenced isolates of S. pyogenes Deletion of the QS system's transcriptional
193 on that promoted opsonophagocytic killing of S. pyogenes in vitro and provided passive immunity in vi
196 resistant isolates from a recent outbreak of S. pyogenes infection in Pittsburgh and in the Lancefiel
197 ibution the toxin has to the pathogenesis of S. pyogenes and that both versions of SPN play an import
201 as titin and fibronectin, the giant pili of S. pyogenes evolved to abrogate mechanical extensibility
202 otide preferences at the seventh position of S. pyogenes Cas9 PAM (5'-NGRNNNT-3'), which was experime
203 -moving outbreak highlights the potential of S. pyogenes to cause a range of diseases in the puerperi
204 comparing genomewide transcript profiles of S. pyogenes NZ131 and isogenic derivative NZ131 rgg duri
208 re supernatants from multiple M serotypes of S. pyogenes isolates or a commercially available SLS pre
211 ased SpeB production in a clinical strain of S. pyogenes and relieved its growth phase dependency.
212 re infected in thigh muscle with a strain of S. pyogenes that expresses a high level of SpyCEP, or wi
213 plete genome sequences of the type strain of S. pyogenes will effectively serve as valuable taxonomic
214 aspA gene from two different M28 strains of S. pyogenes abrogated their abilities to form biofilms o
216 nteractions in attachment of skin strains of S. pyogenes to keratinocytes are unique and remain unide
221 factor SpeB and to the overall virulence of S. pyogenes, as both DacA and Pde2 null mutants were hig
225 ed a locus that is highly conserved in other S. pyogenes genomes and is homologous to an operon invol
228 ss the need for a refined model of prolonged S. pyogenes asymptomatic mucosal colonization, we have a
229 tive form of the streptococcal CXC protease, S. pyogenes cell envelope proteinase, we developed a com
231 The polC gene from Streptococcus pyogenes (S. pyogenes, strain SF370) has been cloned and expressed
235 s question, we discovered that all sequenced S. pyogenes strains possess the genes for the malic enzy
237 ed in a small subset of patients with severe S. pyogenes sepsis but not in patients with any other ca
238 lar pathogen carriage by phagocytes, we show S. pyogenes remain extracellular during transit, first i
239 st report to show natural induction of sigX, S. pyogenes remained nontransformable under laboratory c
243 AS; Streptococcus pyogenes) protease SpyCEP (S. pyogenes cell envelope protease) cleaves granulocyte
248 These findings justify the hypothesis that S. pyogenes infections are more important in the pathoge
254 Remarkably, these observations suggest that S. pyogenes uses SAgs to manipulate Vbeta-specific T cel
258 and Northern blot analyses to determine the S. pyogenes mRNA half-life of the transcriptome and to u
259 we examined the role of GacB, encoded in the S. pyogenes GAC gene cluster, in the GAC biosynthesis pa
260 two-cassette system expressing pieces of the S. pyogenes Cas9 (SpCas9) protein which splice together
261 system leverages the programmability of the S. pyogenes Cas9 and is based on flexible arrangements o
262 the highly recombinatorial FCT region of the S. pyogenes genome is under strong selection for change
264 ed the ExPortal, a unique microdomain of the S. pyogenes membrane, specialized for protein secretion
265 rain found that between 3.7 and 28.5% of the S. pyogenes transcripts were differentially expressed, d
267 ts with mutually permissive NGGRRT PAMs, the S. pyogenes Cas9 and S. aureus Cas9 yield indels at comp
268 between a known effector of the pathway, the S. pyogenes NAD(+) glycohydrolase (SPN), and a second se
273 f SLPI and lysozyme would be advantageous to S. pyogenes in establishing colonization on mucosal surf
274 ontrol protein modules 6 and 7) that bind to S. pyogenes, linked to the Fc region of IgG (FH6-7/Fc).
275 d structures of AcrIIA2 or AcrIIA2b bound to S. pyogenes Cas9 reveal a mode of competitive inhibition
277 ther, these data show that ME contributes to S. pyogenes' carbon source repertory, that malate utiliz
279 tion of Tn5-based transposome mutagenesis to S. pyogenes with initial screening for reduced expressio
280 ate that caspase-1 activation in response to S. pyogenes infection requires NF-kappaB and the virulen
282 both SPN and SLO contribute significantly to S. pyogenes pathogenesis in these virulence assays.
283 subsp. equisimilis isolates were similar to S. pyogenes isolates, in that strains of the same emm ty
285 a-lactam antibiotics, commonly used to treat S. pyogenes infections, do not readily permeate mammalia
291 tt and Robin Patel of the Mayo Clinic, where S. pyogenes NAATs have been used for well over a decade
292 ests a novel mechanism of virulence by which S. pyogenes uses its metabolism to modulate innate immun
294 design rules and paired S. aureus Cas9 with S. pyogenes Cas9 to achieve dual targeting in a high fra
299 recombinant S5nA acted synergistically with S. pyogenes nuclease A to generate macrophage-toxic deox