ライフサイエンスコーパス: SERCA

1 SERCA [sarco(endo)plasmic reticulum calcium ATPase] is r

2 SERCA activity in muscle can be regulated by phospholamb

3 SERCA activity is regulated by a variety of small transm

4 SERCA block likely produces mild SR depletion in normal

5 SERCA function remains constant during compensated hyper

6 SERCA function remains constant in CH but decreases (tau

7 SERCA inhibition was maximally relieved by P16-PLB (the

8 SERCA resides in an environment made up largely by the l

9 SERCA uses separate proton and metal ion pathways during

10 SERCA-mediated Ca(2+) uptake was measured with the ER-ta

11 SERCAs (sarco-endoplasmic reticulum Ca(2+)-ATPases) pump

12 In addition to transporting Ca(2+), SERCA facilitates bidirectional proton transport across

13 that mitochondria supply ATP to the ER and a SERCA-dependent Ca(2+) gradient across the ER membrane i

14 f SERCA, though the PLN oligomer straddles a SERCA-SERCA crystal contact.

15 e with a model in which PLB interacts with a SERCA homodimer in a stoichiometry of 1:2.

16 ) in ICC, and blocking Ca(2+) release with a SERCA inhibitor (thapsigargin) or a store-operated Ca(2+

17 MetS abolished SERCA activation by GLP-1 receptor agonists.

18 e GLP-1 receptor agonist exenatide activated SERCA but did not alter other Ca(2+) transporters.

19 Glucose metabolism also activates SERCA pumps, which fills the endoplasmic reticulum and h

20 PLN inhibition is length-dependent, allowing SERCA activity to be restored incrementally.

21 elation between oligomerization affinity and SERCA-binding.

22 TBBPA activated RyR1 and inhibited DHPR and SERCA, inducing a net efflux of Ca(2+) from loaded micro

23 to quantify micropeptide oligomerization and SERCA-binding.

24 quaternary conformation of PLB pentamer and SERCA-PLB regulatory complex.

25 e energy transfer (FRET) from PLB to PLB and SERCA to PLB, suggesting a change in quaternary conforma

26 e, molecular dynamics simulations of SLN and SERCA interaction showed a rearrangement of SERCA residu

27 strated a novel interaction between WFS1 and SERCA by co-immunoprecipitation in Cos7 cells and with e

28 The sarcoplasmic reticulum Ca(2+)-ATPase SERCA promotes muscle relaxation by pumping calcium ions

29 tory membrane proteins of the calcium ATPase SERCA, namely sarcolipin and phospholamban, in explicit

30 (3)H]ryanodine binding and SR Ca(2+) ATPase (SERCA) activity were also tested.

31 d sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) activity.

32 nd sarcoendoplasmic reticulum Ca(2+) ATPase (SERCA) activity.

33 THADA binds the sarco/ER Ca(2+) ATPase (SERCA) and acts on it as an uncoupler.

34 co/endoplasmic reticulum (SR) Ca(2+) ATPase (SERCA) and is abnormally elevated in the muscle of Duche

35 tor of sarcoplasmic reticulum Ca(2+) ATPase (SERCA) in muscle.

36 a sarcoendoplasmic reticulum Ca(2+) ATPase (SERCA) inhibitor, on a panel of unselected patient-deriv

37 ion of sarcoplasmic reticulum Ca(2+) ATPase (SERCA) pump is one of the key determinants of the phenot

38 sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA) pump, could contribute to heat production in skel

39 d sarco/endoplasmic reticulum Ca(2+) ATPase (SERCA)-mediated reuptake rather than changes in Ca(2+) i

40 plasmic/endoplasmic reticulum Ca(2+) ATPase (SERCA).

41 sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA).

42 er sarcoendoplasmic reticulum Ca(2+) ATPase (SERCA).

43 sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) activity, (2) CAMKII modulation of SERCA, L-type

44 sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) as the principal regulators of systolic and diast

45 sarco-endoplasmic reticulum Ca(2+) -ATPase (SERCA) at the propagation front elevates local [Ca(2+) ]

46 sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) pump and blockers of inositol triphosphate recept

47 sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA) pump is necessary for maintenance of spontaneity.

48 sarco/endoplasmic reticulum Ca(2+) -ATPase (SERCA).

49 rcoendoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA) activity.

50 ctions by repressing sarco/ER Ca(2+)-ATPase (SERCA) activity.

51 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) and are implicated in breast cancer and Hailey-Ha

52 plasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA) and Na(+), K(+)-ATPase.

53 Sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA) and phospholamban (PLB) are essential for intrace

54 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) and phospholamban (PLN) complex regulates heart r

55 a sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) Ca(2+) pump inhibitor, reproducibly displayed sig

56 m pump sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) counter-transports Ca(2+) and H(+) at the expense

57 e sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) has emerged as a major contributor to ER stress.

58 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) is a P-type ATPase that transports Ca(2+) from th

59 he sarcoendoplasmic reticulum Ca(2+)-ATPase (SERCA) is responsible for intracellular Ca(2+) homeostas

60 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) protein expression or activity was not altered, i

61 irectly binds to the sarco/ER Ca(2+)-ATPase (SERCA) pump at the ER, changing its oxidative state and

62 e sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump during Ca(2+) refilling of the ER.

63 e sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump is a major regulatory axis in cardiac muscle

64 The sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) transports two Ca(2+) ions from the cytoplasm to

65 f sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) was tissue-specifically knocked down.

66 pump (sarcoplasmic reticulum Ca(2+)-ATPase (SERCA)) in complex with phospholamban (PLB).

67 Sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA), a member of the P-type ATPases family, transport

68 , sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA), and decreases levels of the pro-apoptotic protei

69 co/endoplasmic reticulum (ER) Ca(2+)-ATPase (SERCA), disrupts Ca(2+) homeostasis, and causes cell dea

70 he sarcoendoplasmic reticulum Ca(2+)-ATPase (SERCA), which plays a lead role in muscle contractility.

71 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA).

72 ed back into the SR by the SR Ca(2+)-ATPase (SERCA).

73 sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA)2a signalling and decreased myocardial energy meta

74 rcoendoplasmic reticulum Ca(2+)alpha ATPase (SERCA) expression is downregulated and mitochondrial fun

75 The sarco/endoplasmic reticulum Ca ATPase (SERCA) pump then refills SR Ca stores.

76 d/or decreased activity of the SR Ca ATPase (SERCA).

77 the sarco-/endoplasmic reticulum Ca-ATPase (SERCA) pump, inositol-1,4,5-triphosphate receptor (IP3R)

78 ity of the sarcoplasmic reticulum Ca-ATPase (SERCA).

79 coplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) activity was reduced and western blot analysis sh

80 s of the sarcoplasmic reticulum Ca2+ ATPase (SERCA) regulatory protein sarcolipin, which is predomina

81 ncreased sarcoplasmic reticulum Ca2+-ATPase (SERCA) Ca2+ reuptake, modulated by increased phospholamb

82 sarco/endoplasmic reticulum calcium ATPase (SERCA) establishes the intracellular calcium gradient ac

83 sarco(endo)plasmic reticulum calcium ATPase (SERCA) in cardiac myocytes is modulated by an inhibitory

84 tor of the endo/sarcoplasmic calcium ATPase (SERCA), is presented.

85 sarco/endoplasmic reticulum calcium ATPase (SERCA), which plays a key role in the maintenance of Ca(

86 sarco/endoplasmic reticulum calcium ATPase (SERCA).

87 lasmic/endoplasmic reticulum calcium ATPase (SERCA).

88 butions of RyRs and sarco/ER calcium-ATPase (SERCA) pumps that we predict using a computational model

89 sarco/endoplasmic reticulum calcium-ATPase (SERCA).

90 ed that sarco(endo)plasmic reticulum ATPase (SERCA) expression was elevated in several WFS1-depleted

91 dulation of the SR Ca(2+)-stimulated ATPase (SERCA) and RyRs by K201.

92 doplasmic reticulum calcium trasport ATPase (SERCA) pump activity with thapsigargin prolonged NMDAR-D

93 doplasmic reticulum calcium trasport ATPase (SERCA) pump prolonged NMDAR-DeltaCa(2+) responses in sha

94 In myocytes from SERCA knock-in mice, basal SERCA activity and SR calcium content were decreased.

95 N pentamer and the novel interaction between SERCA and an SLN monomer.

96 noted multiple modes of interaction between SERCA and phospholamban and observed that once a particu

97 ormational memory in the interaction between SERCA and phospholamban, thus providing insights into th

98 microscopy to study the interaction between SERCA and PLN.

99 d peptide can target and constitutively bind SERCA.

100 om Ca(2+)-bound SERCA, SLN continues to bind SERCA throughout its kinetic cycle and promotes uncoupli

101 lthough PLB gets dislodged from Ca(2+)-bound SERCA, SLN continues to bind SERCA throughout its kineti

102 found that the transport sites of PLB-bound SERCA are completely exposed to the cytosol and that K(+

103 to determine structural changes of PLB-bound SERCA in response to binding of a single Ca(2+) ion.

104 pal component analysis showed that PLB-bound SERCA lies exclusively along the structural ensemble of

105 normal intracellular pH (7.1-7.2), PLB-bound SERCA populates an E1 state that is deprotonated at resi

106 calcium translocation and ATP hydrolysis by SERCA under conditions that mimic environments in sarcop

107 ying the active transport of calcium ions by SERCA.

108 and that dimer formation is not modulated by SERCA conformational poise, PLB binding, or PLB phosphor

109 tered ATP-dependent calcium translocation by SERCA within the first transport cycle, whereas sarcolip

110 ole in optimizing active Ca(2+) transport by SERCA.

111 results from the leak opposing Ca uptake by SERCA.

112 is accelerated by depletion of the cellular SERCA-like Pmr1 ATPase-driven Ca(2+)/Mn(2+) pump, regula

113 ny with its distinct role as a PLB-competing SERCA activator, in contrast to the inhibitory function

114 ent measurements, proteoliposomes containing SERCA and phospholamban or sarcolipin were adsorbed to a

115 However, despite decreased SERCA-PLB binding, intermolecular FRET for Val(49)-stop

116 conclude that R9C mutation of PLB decreases SERCA inhibition by decreasing the amount of the regulat

117 k and junctional SR, cytosolic Ca diffusion, SERCA uptake activity, and RyR open probability.

118 ed intermediate of the pump populated during SERCA regulation.

119 no therapeutics that directly target either SERCA or PLN.

120 alizes to the SR membrane, where it enhances SERCA activity by displacing the SERCA inhibitors, phosp

121 Aside from clinical features, SERCA activity measurement and SERCA1 western blot can a

122 ing the leaked SR Ca(2+) more accessible for SERCA.

123 in the binding affinity of truncated PLB for SERCA and loss of inhibitory potency.

124 increase in binding affinity of V49A-PLB for SERCA, and a gain of inhibitory function as quantified b

125 emains constant in CH but decreases (tau for SERCA-mediated Ca(2+) removal changed from 6.3 to 3.0 s(

126 roximately 0.5 muM) was much larger than for SERCA (IC50 > 285 muM).

127 In both crystal forms, SERCA molecules are organized into identical antiparalle

128 n for decades as an assembly of calcium-free SERCA molecules induced by the addition of decavanadate.

129 In myocytes from SERCA knock-in mice, basal SERCA activity and SR calcium

130 g membrane repolarization and alterations in SERCA activity that reduce cardiomyocyte contractility.

131 Hence, we hypothesized that a decrease in SERCA pump expression and/or activity in lymphatic muscl

132 ng but explained by the observed increase in SERCA activity.

133 olic failure, all of which were inhibited in SERCA knock-in mice.

134 xistence of a transient water-filled pore in SERCA that connects the Ca(2+) binding sites with the lu

135 a distinct structural and functional role in SERCA regulation.

136 Large-scale transitions in SERCA occur at time-scales beyond the current reach of u

137 traction even when it successfully increases SERCA expression.

138 an (PLN) and sarcolipin (SLN), which inhibit SERCA, the membrane pump that controls muscle relaxation

139 some Pen derivatives significantly inhibited SERCA at concentrations required to modulate RyRs.

140 i and colleagues demonstrate that inhibiting SERCA calcium pumps preferentially impairs the maturatio

141 ally occurring p53 missense mutants inhibits SERCA pump activity at the ER, leading to a reduction of

142 n that unphosphorylated PLB (U-PLB) inhibits SERCA and that phosphorylation of PLB at Ser-16 or Thr-1

143 ies alone have led to a greater insight into SERCA-PLB regulation, the structural mechanisms for Ca(2

144 We investigated SERCA dimerization in rabbit left ventricular myocytes u

145 impaired cellular signaling steps involving SERCA.

146 t phosphorylation may lead to longer-lasting SERCA stimulation and may sustain maladaptive Ca(2+) han

147 Western blotting of cross-linked SERCA revealed higher-molecular-weight species consisten

148 graphy studies have suggested that PLB locks SERCA in a low-Ca(2+)-affinity E2 state that is incompat

149 Lowering SERCA level will enable intracellular calcium oscillatio

150 a mechanistic understanding of PLB-mediated SERCA transport regulation.

151 structural mechanisms by which SLN modulates SERCA-dependent contractility and thermogenesis remain u

152 lish an inhibitory interaction with multiple SERCA conformational states with distinct effects on SER

153 n a crystal contact that bridges neighboring SERCA dimer ribbons.

154 d by phospholamban removal, which normalized SERCA function.

155 lation domain and the cytosolic ends of 5 of SERCA's 10 transmembrane helices.

156 or MG132 resulted in reduced accumulation of SERCA levels compared with wild-type cells.

157 Redox activation of SERCA C674 regulates basal SR calcium content, thereby m

158 Moreover, activation of SERCA promotes fusion in a BafilomycinA1-sensitive manne

159 Ca(2+)-dependent activation of SERCA-PLB provides a control function that regulates cyt

160 necessary for Ca(2+)-dependent activation of SERCA.

161 The activity of SERCA is regulated by small membrane protein subunits, t

162 y of SLN to decrease the maximal activity of SERCA, which is distinct from the ability of PLN to incr

163 pling skeletal muscle tissue for analyses of SERCA activity as well as gene expression of SERCA1a and

164 The multiscale correlation of SERCA group (scaling exponent: 0.77 +/- 0.07), on the ot

165 ll helical crystals and large 2D crystals of SERCA in the absence and presence of PLN.

166 ext, we analyzed two-dimensional crystals of SERCA in the presence of wild-type SLN by electron cryom

167 echanism, SLN promotes the futile cycling of SERCA, contributing to muscle heat production.

168 on details of the structural determinants of SERCA regulation have been elusive because of the dynami

169 Diversification of SERCA regulators was much less extensive, indicating the

170 tions to evaluate the structural dynamics of SERCA-PLB in a solution containing 100 mM K(+) and 3 mM

171 mitochondrial function and the efficiency of SERCA in HF.

172 hat oligomerization occurs at the expense of SERCA-binding.

173 face proximal to the calcium entry funnel of SERCA.

174 uptake, likely due to S-glutathionylation of SERCA pumps.

175 ailability attenuated S-glutathionylation of SERCA, resulting in an increase in cytosolic calcium, en

176 ling model involves reversible inhibition of SERCA by monomeric PLN and storage of PLN as an inactive

177 h strong affinity and relieves inhibition of SERCA in a length-dependent manner.

178 d PLN strongly and relieve PLN inhibition of SERCA to a greater extent than a similar length random s

179 of the headpiece underlie PLB inhibition of SERCA, and binding of a single Ca(2+) ion is sufficient

180 201 displayed Ca(2+)-dependent inhibition of SERCA-dependent ATPase activity, which was measured in m

181 rongly (Kd <10 nm) and relieve inhibition of SERCA.

182 rstand the significance of altered levels of SERCA, IP3R, and RyR on the intracellular calcium dynami

183 cs can be modified by changing the levels of SERCA, IP3R, and/or RyR.

184 phosphomimetic R9C-PLB oxidation and loss of SERCA inhibition, leading to impaired calcium regulation

185 interacts with transmembrane segments M3 of SERCA and participates in a crystal contact that bridges

186 pentameric form of PLN interacts with M3 of SERCA and that it plays a distinct structural and functi

187 to interact with transmembrane segment M3 of SERCA, although the interaction appeared to be indirect

188 omeric form of PLN also interacts with M3 of SERCA, though the PLN oligomer straddles a SERCA-SERCA c

189 approaches to develop a structural model of SERCA-PLB interactions to gain a mechanistic understandi

190 mportance to guide therapeutic modulation of SERCA and other evolutionarily related ion-motive ATPase

191 reveal a major role for CAMKII modulation of SERCA in the peak Ca(2+) -frequency response, driven mos

192 e (SERCA) activity, (2) CAMKII modulation of SERCA, L-type channel and transient outward K(+) current

193 y discovered compounds with the potential of SERCA inhibition, discusses their mechanism of action, a

194 LN using co-reconstituted proteoliposomes of SERCA and SLN.

195 echanism for increasing the turnover rate of SERCA.

196 SERCA interaction showed a rearrangement of SERCA residues that is altered when the SLN N terminus i

197 We conclude that PLB-mediated regulation of SERCA activity in the heart results from biochemical and

198 role for WFS1 in the negative regulation of SERCA and provide further insights into the function of

199 anner similar to that of known regulators of SERCA activity, phospholamban (PLB) and sarcolipin (SLN)

200 FXYDs and regulators of SERCA are present in fishes, as well as terrestrial vert

201 We propose that reversal of SERCA-PLB inhibition is achieved by stringing together i

202 e crystals are antiparallel dimer ribbons of SERCA, known for decades as an assembly of calcium-free

203 y and structural dynamics of the E2 state of SERCA.

204 romoting particular conformational states of SERCA, we found that the effect of phospholamban on SERC

205 n calcium cycling and compared with those of SERCA inhibition.

206 slow (millisecond) structural transitions of SERCA, the existence of simultaneous metal and proton pa

207 al regions of SLN that mediate uncoupling of SERCA, we employed mutagenesis and generated chimeras of

208 VF was not observed with an upregulation of SERCA, a potential drug therapy, using the same protocol

209 Platelets exhibit 2 types of SERCAs, SERCA2b and SERCA3 (SERCA3 deficient mice), whic

210 Moreover, the unique oligomerization/SERCA-binding profile of DWORF is in harmony with its di

211 domain to human PLN had a minimal effect on SERCA inhibition.

212 f phospholamban and its regulatory effect on SERCA transport activity.

213 diac tissue, and their functional effects on SERCA have not been determined directly.

214 nformational states with distinct effects on SERCA's kinetic properties.

215 to the effects of annular and bulk lipids on SERCA activation, but the role of a nonannular lipid sit

216 we found that the effect of phospholamban on SERCA depends on substrate preincubation conditions.

217 interacts with a specific inhibitory site on SERCA, and low-resolution structural evidence suggests t

218 ive affinities of PLB for candidate sites on SERCA.

219 interacts with distinct alternative sites on SERCA.

220 specificity became more ambivalent for other SERCA conformers.

221 rast to the inhibitory function of the other SERCA-binding micropeptides.

222 ant flies rescues their obesity, pinpointing SERCA as a key effector of THADA function.

223 effect of R9C on PLB oligomerization and PLB-SERCA binding.

224 ge effects on PLB pentamer structure and PLB-SERCA regulatory complex conformation, increasing and de

225 o SERCA and altered the structure of the PLB-SERCA regulatory complex.

226 tem studies may relate to potentially potent SERCA block under resting Ca(2+) conditions.

227 e of SR Ca(2+) loading, suggesting potential SERCA block and/or RyR agonism.

228 ghly charged transport site, thus preserving SERCA's structural stability during active Ca(2+) transp

229 c-stimulated cardiomyocytes led to prolonged SERCA activation, presumably because 14-3-3 protected PL

230 In addition, the ER transmembrane proteins SERCA and calnexin were not detected in viroplasm-associ

231 phenocopied by depletion of the Ca(2+) pump SERCA, a secondary target of this drug.

232 The calcium pump SERCA is a transmembrane protein that is critical for ca

233 as adenylate kinase, ATP-driven calcium pump SERCA, leucine transporter and glutamate transporter sho

234 ks the sarco/endoplasmic Ca(2+) ATPase pump (SERCA 2), depleting the SER of calcium.

235 ate the sarcoplasmic reticulum calcium pump (SERCA) by relieving its inhibition by PLN.

236 The sarcoplasmic reticulum calcium pump (SERCA) is regulated by the small integral membrane prote

237 Reduced SERCA activity underlies dysregulation of Ca(2+) homeost

238 Ca(2+) clearance and relaxation and reduced SERCA activity.

239 This research suggests that reduced SERCA level is the main factor responsible for the reduc

240 Reducing SERCA activity in THADA mutant flies rescues their obesi

241 r, how phospholamban and sarcolipin regulate SERCA is not fully understood.

242 d PLB without losing the ability to regulate SERCA activity; however, the resulting chimeras acquire

243 structural changes and motions that relieve SERCA inhibition by PLB.

244 SLN gene normalizes SLN expression, restores SERCA function, mitigates skeletal muscle and cardiac pa

245 ractive partner of the endoplasmic reticulum SERCA pumps and treatment with the SERCA-inhibitor Thaps

246 To date, several SERCA inhibitors have been thoroughly studied and the mo

247 We modeled the binding of PLB to several SERCA conformations, representing different enzymatic st

248 phospholamban, the other well studied small SERCA-regulatory proteins, oligomerize either alone or t

249 Results demonstrated that SERCA inhibitor, thapsigargin, significantly reduced lym

250 eactive oxygen species, we hypothesized that SERCA oxidation at C674 would modulate the effects of re

251 It has been proposed that SERCA forms homooligomers that increase the catalytic ra

252 zation of this isoform (zfPLN) revealed that SERCA inhibition and reversal by phosphorylation were co

253 fluorescently labeled SERCA2a revealed that SERCA readily forms homodimers.

254 The data suggest that SERCA calcium binding induces the pump to undergo a tran

255 Together, these results suggest that SERCA forms constitutive homodimers in live cells and th

256 The SERCA (sarco-endoplasmic reticulum Ca(2+) ATPase) inhibi

257 The SERCA activator, CDN 1163 partially restored lymphatic c

258 The SERCA group shows longer heart beat intervals (Mean +/-

259 nly endogenous peptide known to activate the SERCA pump by physical interaction and provides a means

260 it enhances SERCA activity by displacing the SERCA inhibitors, phospholamban, sarcolipin, and myoregu

261 Mice with heterozygous knock-in of the SERCA C674S mutation were subjected to chronic ascending

262 ovel sites on M3 and with the outside of the SERCA helix M9.

263 e structure and functional properties of the SERCA mutant E340A.

264 apsigargin, an irreversible inhibitor of the SERCA pump, exhibited anxiogenic-like behaviors and incr

265 terminant of the quaternary structure of the SERCA regulatory complex.

266 ers formed in the absence or presence of the SERCA regulatory partner, phospholamban (PLB) and were u

267 Agonist-stimulated phosphorylation of the SERCA regulatory protein phospholamban was increased in

268 A projection map of the SERCA-SLN complex was determined to a resolution of 8.5

269 iation is engaged it persists throughout the SERCA transport cycle and multiple turnover events.

270 er-regulin (ALN) are reported to bind to the SERCA calcium pump in a manner similar to that of known

271 ection was abolished by cotreatment with the SERCA inhibitor cyclopiazonic acid.

272 reticulum SERCA pumps and treatment with the SERCA-inhibitor Thapsigargin halted intracellular MRSA s

273 This SERCA-SLN complex correlated with the ability of SLN to

274 a(2+) to LCC density and diastolic Ca(2+) to SERCA density decreased by 16-fold and increased by 23%,

275 availability of the micropeptide to bind to SERCA in a regulatory complex, we used co-immunoprecipit

276 The R9C also decreased PLB binding to SERCA and altered the structure of the PLB-SERCA regulat

277 optosis and autophagy by directly binding to SERCA and causing endoplasmic reticulum (ER) stress and

278 We propose that PLB binding to SERCA populates a novel (to our knowledge) E1 intermedia

279 SLN binding to SERCA uncouples Ca(2+) transport from ATP hydrolysis.

280 merization are also important for binding to SERCA.

281 de that the luminal extension contributes to SERCA inhibition but only in the context of zfPLN.

282 ine (non-sensitising) had similar effects to SERCA inhibition: decreased systolic [Ca(2+)]i , increas

283 uptake by the Ca(2+)adenosine triphosphatase SERCA.

284 c reticulum Ca(2+) adenosine triphosphatase (SERCA)2a, a critical regulator of calcium homeostasis, i

285 at ventricular myocytes expressing wild-type SERCA, H(2)O(2) caused a 25% increase in mitochondrial c

286 fect of C674 oxidation on apoptosis in vivo, SERCA knock-in mice were subjected to chronic ascending

287 However, the exact mechanisms whereby SERCA inhibition induces cell death are incompletely und

288 Ca transient in the majority of cells while SERCA inhibition produced monophasic decay.

289 reduces its contractibility and explain why SERCA gene therapy, a change in calcium handling to trea

290 monstrating that PLN remains associated with SERCA and that the PLN pentamer is required for the regu

291 at during rest NCX effectively competes with SERCA for cytosolic Ca(2+) that leaks from the SR.

292 Meanwhile, new compounds with SERCA-inhibiting properties of natural, synthetic, or se

293 her-molecular-weight species consistent with SERCA oligomerization.

294 MLN interacts directly with SERCA and impedes Ca(2+) uptake into the SR.

295 eptide species still showed robust FRET with SERCA, and there was a surprising positive correlation b

296 rm of all micropeptides that interacted with SERCA.

297 ccess to potential sites of interaction with SERCA.

298 SLN physically interacts with SERCA and differentially regulates contractility in skel

299 , we show that a PLN oligomer interacts with SERCA in a similar manner in both crystal forms.

300 etic peptides in phospholipid membranes with SERCA and measured calcium-dependent ATPase activity.