ライフサイエンスコーパス: SH3 domain

1 up to nine spectrin repeats (SR1-SR9) and an SH3 domain.

2 a groove identified under the RT loop of the SH3 domain.

3 n the local environments upon binding to the SH3 domain.

4 c tail of the beta3 integrin subunit via its SH3 domain.

5 ft perturbations on the opposite face of the SH3 domain.

6 development does not require the C-terminal SH3 domain.

7 that overlaps with PPII binding site in its SH3 domain.

8 conserved Nef PXXPXR motif engaging the Hck SH3 domain.

9 fibrils occur by a domain-swap mechanism in SH3 domain.

10 ed of 95 mol% of zwitterionic lipids via the SH3 domain.

11 izing PEGylation site within the chicken Src SH3 domain.

12 binding proteins derived from the human Fyn SH3 domain.

13 of dAbp1, which are mediated largely by its SH3 domain.

14 a function-blocking mutation into the dAbp1 SH3 domain.

15 an inhibitory function exerted by plectin's SH3 domain.

16 the SKAP-Hom Y260 and Y297 residues and its SH3 domain.

17 e Pro-rich motif with acidic clusters in the SH3 domain.

18 in, ITK interacts with TSAD only through its SH3 domain.

19 eting of Ptc1p to proteins recognized by the SH3 domain.

20 by amino acid substitutions in the adjacent SH3 domain.

21 tion motif in a linker between the first two SH3 domains.

22 globular domains corresponding to the three SH3 domains.

23 ated by the kindlin-2 F0 and the Src SH2 and SH3 domains.

24 e that is up-regulated by N-terminal SH2 and SH3 domains.

25 ional changes in both the ankyrin repeat and SH3 domains.

26 insights into the recognition mechanisms of SH3 domains.

27 unctions have been assigned to the F-BAR and SH3 domains.

28 expressed with or without a src homology 3 (SH3) domain.

29 through their SRC homology 2 and 3 (SH2 and SH3) domains.

30 h region (PRR) through their Src homology 3 (SH3) domains.

31 CD2AP SH3 domains 1 and 2 generally bound with similar charact

32 resistant Nck1(K178R) or Nck2 containing the SH3 domain 2 of Nck1 restores stress fibres in synaptopo

33 the steps of binding between the yeast Abp1p SH3 domain (AbpSH3) and a proline-rich IDP, ArkA.

34 ntrol F-BAR-membrane interactions as well as SH3 domain activities, and suggest that these two functi

35 region (AIR) and the tandem Src homology 3 (SH3) domains, allowing the AIR to undergo phosphorylatio

36 ycete fungal genomes and that all possess an SH3 domain and a conserved novel Ptc1p binding motif.

37 More interestingly, beta1a SH3 domain and C terminus, when simultaneously engineere

38 directly to FAK PRR2 and PRR3 sites via its SH3 domain and cortactin expression is important in prom

39 tion and showed direct binding between dAmph-SH3 domain and dNedd4Lo N-terminus.

40 Tp53BP2 encodes ASPP2 (ankyrin repeats, SH3 domain and protein rich region containing protein 2)

41 inimally, this interaction requires UNC-89's SH3 domain and residues 294-376 of paramyosin and has a

42 A fragment comprising the SH3 domain and the guanylate kinase domain of synapse-as

43 synaptic proteins are known to bind to this SH3 domain and to thereby activate gephyrin clustering.

44 nonnative hydrophobic interactions for other SH3 domains and IDPs in general.

45 protein family that contains an SH2 and two SH3 domains and is involved in signal transduction from

46 rstand the interaction between the N-BAR and SH3 domains and its effect on biological function.

47 sted this prediction, using the pentamers of SH3 domains and proline-rich motifs (SH3(5) and PRM(5))

48 erent CaV isoforms as a binding site for the SH3 domains and report a crystal structure of the comple

49 distinct from others observed previously in SH3 domains and, to our knowledge, this is the first exa

50 ng F-BAR domain as well as a Src homology 3 (SH3) domain and a G protein-binding homology region 1 (H

51 vealed a pivotal role of the src homology 3 (SH3) domain and C terminus of beta1a in charge movement

52 DHHC6, contains a predicted Src-homology 3 (SH3) domain and DHHC6 is localized to the ER membrane.

53 IP1-R405C mutation is in the SRC homology 3 (SH3) domain and impairs Wiskott-Aldrich syndrome protein

54 of-function mutations in the Src homology 3 (SH3) domain and tetratricopeptide repeats 2 (SH3TC2) gen

55 o a number of different proteins through its SH3 domain, and a region N-terminal to the SH3 domain bi

56 ly through weak interactions with the second SH3 domain, and this effect appears to promote phase sep

57 with signature sequences of ankyrin repeat-, SH3 domain-, and proline-rich region-containing protein

58 Arf GTPase-activating protein (Arf GAP) with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) estab

59 ADP ribosylation factor 4 (Arf4)/ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)-depen

60 studies indicate that ACK1 and the cortactin SH3 domain are essential for ligand-mediated EGFR intern

61 SH3 domains are common protein interaction domains that

62 SH3 domains are one of the largest and most well-charact

63 identified the Nck1 SH2 domain and the first SH3 domain as critical for flow-induced endothelial acti

64 n the transition-state ensemble (TSE) in src SH3 domain as f is increased.

65 t containing distinct classes of ligands for SH3 domains, as well as on a new, an order of magnitude

66 iously studied tight complexes, we find that SH3 domain association rates are enhanced by long range

67 Experimental aggregation of Nck SH3 domains at the membrane induces actin comet tails--d

68 o better understand the connectivity of this SH3 domain-based protein network at the CR and its funct

69 We show in atomic detail that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of Aplip1/J

70 Src homology 3 (SH3) domains bind proline-rich linear motifs in eukaryot

71 tion and angiogenesis via directly targeting SH3 domain binding glutamate-rich protein (SH3BGR).

72 interactions are inhibited in the absence of SH3 domain binding ligands such as dynamin's prolin rich

73 -1), TIA1-related protein (TIAR), and RasGAP-SH3 domain binding protein 1 (G3BP1) are required for ef

74 lutamic acid did not alter BCAR3-induced Src SH3 domain binding to p130(cas).

75 Therefore, anionic lipids can modulate TIL/SH3 domain binding.

76 these two proteins block augmentation of Src SH3 domain binding.

77 entified in this screen is a RhoGAP protein, SH3-domain binding protein 1 (SH3BP1).

78 at is associated with point mutations in the SH3-domain binding protein 2 (SH3BP2) gene, which encode

79 localisation with GTPase Activating Protein (SH3 domain) Binding Proteins (G3BPs).

80 In addition, cyclophilin A increased Crk SH3 domain-binding guanine-nucleotide releasing factor (

81 ective interactions that conform to class II SH3 domain-binding peptides.

82 TYMP contains an N-terminal SH3 domain-binding proline-rich motif and forms a comple

83 SG-nucleating protein Ras-GTPase activating SH3 domain-binding protein (G3BP1), which is mediated by

84 ere we report that GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) is critical for DNA

85 Here, the Ras-GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) was identified as a

86 Here, we identify SH3 domain-binding protein 4 (SH3BP4) as a binding prote

87 irst report the discovery of Src homology 3 (SH3) domain-binding glutamic acid-rich-like protein (SH3

88 rotein 3 (nsP3) forms a complex with Ras-GAP SH3-domain-binding protein (G3BP) and sequesters it into

89 vel, we identify Ras-GTPase-activing protein SH3-domain-binding protein 2 (G3BP2) as an alpha-parvin-

90 s SH3 domain, and a region N-terminal to the SH3 domain binds to the protein phosphatase, Ptc1p.

91 RBP, via its C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport adaptor inv

92 ced form of Brk (Alt Brk) which contains its SH3 domain blocks pY88 and acts as an endogenous cdk4 in

93 he mutation does not cause misfolding of the SH3 domains, but abolishes the interaction.

94 CD2AP binding sites, recognized by all three SH3 domains, but SH3-3 appeared non-functional in precip

95 el systems are well-conserved in other human SH3 domains, but that the corresponding tyrosines are kn

96 of the archetypal F-BAR domain linked to an SH3 domain by an intrinsically disordered region (IDR).

97 The ASPP2 SH3 domain can discriminate between PP1 isoforms using a

98 CB2 harboring the N-terminal Src homology 3 (SH3) domain (CB2SH3+) adopts a closed and autoinhibited

99 old protein in TLR4 signaling called SAM and SH3 domain containing protein 1 (SASH1).

100 of Src homology-2 (SH2) and Src homology-3 (SH3) domain containing proteins that controls the coordi

101 phorylation and nuclear translocation of the SH3-domain containing protein GRB2 and an SH3-domain lig

102 Multiple SH3 domain-containing adapter proteins can bind and poss

103 ctions of BCAP with Src homology 2 (SH2) and SH3 domain-containing adaptor proteins, notably growth f

104 Here we show that ubiquitin-associated and SH3 domain-containing B (UBASH3B), a protein tyrosine ph

105 ences of the Itch PRR toward its most common SH3 domain-containing partners and demonstrate that the

106 Two SH3 domain-containing PCH family members, Cdc15 and Imp2

107 previously showed that FCH/F-BAR and Double SH3 Domain-Containing Protein (FCHSD2) has a cancer-cell

108 SASH1 (SAM and SH3 domain-containing protein 1) is a tumor suppressor p

109 Proteome profiling identified sorbin and SH3 domain-containing protein 2 (SORBS2) and PDZ and LIM

110 p at the 4q35 locus involving the sorbin and SH3 domain-containing protein 2 gene, SORBS2, a skeletal

111 nd characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhab

112 The SH3 domain-containing proteins bind to another fragment

113 ted that the TRD3 binds WD40, WWI, WWII, and SH3 domain-containing proteins.

114 iverging into A and B subgroups, are BAR and SH3 domain-containing proteins.

115 csins but can also target a variety of other SH3 domain-containing proteins.

116 th beta-PIX and a 1:1 complex with the other SH3 domain-containing proteins.

117 proteins and also binds the Src homology 3 (SH3) domain-containing proteins CD2AP and SH3KBP1.

118 own to interact with several Src homology 3 (SH3) domain-containing proteins.

119 Nbp2p is an Src homology 3 (SH3) domain-containing yeast protein that is involved in

120 Vamana, an SH3-domain-containing protein, physically associates wit

121 idues and is generally considered to bind to SH3-domain-containing proteins.

122 IL, charged membrane lipids, BAR domain, and SH3 domain could exist in the biological system and that

123 This process is associated with the SH3 domain-dependent translocation of Dok-3/Grb2 complex

124 R constriction most likely by inhibiting the SH3-domain-dependent interactions of Hof1.

125 vity to allow covalent decoration of natural SH3 domains, depending on choice of catalyst but indepen

126 structures of tyrosine-phosphorylated human SH3 domains derived from the Abelson-family kinases ABL1

127 udies, focused on ketosteroid isomerase, the SH3 domain, dihydrofolate reductase, and cytochrome c, w

128 uncated protein lacking WD40-repeats and the SH3 domain; disease was hitherto attributed to loss of t

129 pecificity for certain domain types, such as SH3 domains distributed across different proteins.

130 On membrane binding, Nwk SH3 domains do not completely dissociate from the F-BAR

131 n single-molecule pulling experiments on src SH3 domain, exhibits upward curvature on a [Formula: see

132 The observed loop motions suggest that the SH3 domain exists in a binding-competent conformation in

133 solution, the isolated MLV IN CTD adopts an SH3 domain fold flanked by a C-terminal unstructured tai

134 in the recognition preferences of its three SH3 domains for c-CBL, ALIX, and RIN3.

135 ry expressing the entire complement of human SH3 domains for novel NS5A-host cell interactions.

136 n a microcrystalline protein (alpha-spectrin SH3 domain), for which we are able to identify and model

137 that the PI3K p85 subunit binds to BCAP via SH3 domains forming an inactive complex that is then act

138 (epsilon)-chloroacetyl lysine) to N-terminal SH3 domain from adapter protein Grb2.

139 nomer cross-interaction between H0 helix and SH3 domain from different subunits within a homodimer.

140 As a proof of principle, we use the SH3 domain from human Fyn kinase to build a sensor that

141 e core packing and ligand recognition of the SH3 domain from human Fyn tyrosine kinase.

142 In the current study, we tested whether SH3 domains from the F-BAR (FCH-Bin-Amphiphysin-Rvs) sub

143 e the binding pathway of the Src homology 3 (SH3) domain from the Fyn tyrosine kinase, which forms co

144 ise analysis identified SNX25, PDLIM3, and 3 SH3 domain genes (SORBS2, SH3RF3, and NPHP1) to be signi

145 horylation at different tyrosine residues in SH3 domains has been reported previously.

146 rising a metastable triple mutant of the Fyn SH3 domain, have been investigated using pressure-depend

147 s the first example of peptide-binding of an SH3 domain in a bacterial system.

148 embranes, highlighting the importance of the SH3 domain in regulating the function of endophilin.

149 We also show that the SH3 domain in the C-terminus plays an important role in

150 l regions of dense bodies via its C-terminal SH3 domains in an ATN-1(alpha-actinin)- and ALP-1(ALP/En

151 eals a requirement for the PACSIN1 F-BAR and SH3 domains in controlling these NMDAR-dependent process

152 nization exposes the binding sites of all 12 SH3 domains in the tetramer, allowing simultaneous bindi

153 the endophilin N-BAR domain (which lacks the SH3 domain including a linker region of the full length

154 F4alpha F domain that interacts with the Src SH3 domain, increase phosphorylation by Src and decrease

155 Domain swaps observed previously in other SH3 domains indicate a general propensity of this protei

156 cal shift perturbations in the (15)N-labeled SH3 domain induced by the C-terminal beta3 tail peptide

157 on a molecular level how the LCK SH3 and ITK SH3 domains interact with TSAD in human HEK293T cells, h

158 e the first evidence for the function of the SH3 domain interaction in synaptic vesicle (SV) organiza

159 the negative regulatory effects mediated by SH3 domain interactions.

160 e studies have highlighted a role for EH and SH3 domain Intersectin (Itsn) proteins in synaptic vesic

161 the Wiskott-Aldrich syndrome protein via its SH3 domain is critical for integrin activation and neutr

162 Thus, the SH3 domain is far less reliant on electrostatic enhancem

163 Although the N-terminal SH3 domain is important for p130Cas localization, it has

164 When the SH3 domain is overexpressed, paramyosin is mislocalized.

165 tation analysis indicates that the cortactin SH3 domain is responsible for binding to ACK1.

166 on between GK and the nearby Src homology 3 (SH3) domain, leading to a closed conformation, whereas a

167 a (SPRR2a), which acts as an src homology 3 (SH3) domain ligand, induces biliary epithelial cell (BEC

168 he SH3-domain containing protein GRB2 and an SH3-domain ligand in ZEB1 is required for SPRR2a-induced

169 d with (15)N CEST experiments recorded on an SH3 domain-ligand exchanging system and subsequently use

170 rdingly, NMR spectroscopy indicated that the SH3 domains may compete for these two adjacent binding s

171 sites in the region between the CRIB-PR and SH3 domains mediate the binding of 14-3-3.

172 These syndapin I functions reflected direct, SH3 domain-mediated associations and functional interact

173 n of the cell wall integrity pathway through SH3 domain-mediated interaction with Bck1p, a component

174 ne mutants (Y260F, Y260F/Y297F), or SKAP-Hom SH3 domain mutant (W335K).

175 Interestingly, the SH3 domain mutant-rescued MEFs showed an enhanced cell m

176 hobic residues on both domains and keeps the SH3 domain near the end of the N-BAR domain, in agreemen

177 omains, the serine-rich region (SRR) and the SH3 domain (NebDelta163-165).

178 y way of miR-200c/141-associated EMT through SH3-domain networks and contributes to benign and malign

179 onfirmed that the peptide first binds to the SH3 domain noncovalently before establishing a covalent

180 f an SH2 domain flanked by N- and C-terminal SH3 domains (nSH3/cSH3).

181 ng point mutation of either the c-Src SH2 or SH3 domain obviates the RANK/alphavbeta3 association.

182 10 and ABI-1 and showed that it requires the SH3 domain of ABI-1.

183 The SH3 domain of Boi2, which is dispensable for bud growth

184 that choline kinase alpha interacts with the SH3 domain of c-Src.

185 ring under the control of the release of the SH3 domain of CB is essential for regulating gephyrin cl

186 n PakB-1-180 that directly interact with the SH3 domain of Dictyostelium actin-binding protein 1 (dAb

187 ne NMR data on the 7-kDa globular N-terminal SH3 domain of Drosophila signal transduction protein drk

188 However, the SH3 domain of endophilin also interacts with the proline

189 We show that the SH3 domain of GRB2 can bind to BCAP independently of BCA

190 ZDHHC19 and STAT3, which is mediated by the SH3 domain of GRB2.

191 LSP1 interacts with the SH3 domain of myosin1e, and the localization and dynamic

192 novel ligand (PDN1) that targets the unique SH3 domain of N1-Src and inhibits N1-Src in cells.

193 nd to N-WASP, by interacting with the second SH3 domain of Nck.

194 Nck1 partners, chimaerins bind to the third SH3 domain of Nck1.

195 We found that the SH3 domain of NOSTRIN is involved in the NOSTRIN-TRAF6 i

196 We demonstrate that only the SH3 domain of p120 selectively inhibits the RhoGAP activ

197 nance, we found eight hits toward the tandem SH3 domain of p47phox (p47phox(SH3A-B)) with K(D) values

198 binding of a Pro-rich peptide ligand to the SH3 domain of phosphatidylinositol 3-kinase unfolded in

199 Direct binding of the SH3 domain of PLC-gamma1 to Pak1 dissociates inactive Pa

200 porter Fps1p, which was shown to require the SH3 domain of Sho1p for binding via its N-terminal solub

201 The SH3 domain of Sho1p was found to be important for bindin

202 Associated molecule with the SH3 domain of signal transduction adaptor molecule (STAM

203 Furthermore, inhibition of the SH3 domain of Son of Sevenless, which is an upstream ada

204 Here, we report that Bag3 interacts with the SH3 domain of Src, thereby mediating the effects of Hsp7

205 exon leads to a 5 or 6 aa insertion into the SH3 domain of Src.

206 tinating enzyme associated molecule with the SH3 domain of STAM (AMSH) is crucial for the removal of

207 The DUB AMSH (associated molecule with the SH3 domain of STAM) has been shown to be involved in reg

208 , a proline-rich peptide in HD-PTP binds the SH3 domain of STAM2.

209 dopsis thaliana associated molecule with the SH3 domain of STAM3 (AMSH3) is a deubiquitinating enzyme

210 n Aim21, which interacts physically with the SH3 domain of the Arp2/3 complex regulator Bbc1.

211 The N-terminal SH3 domain of the Drosophila signal transduction protein

212 mma1 interaction enhances the binding of the SH3 domain of the phospholipase with Pak1.

213 cture-function studies showed that the fifth SH3 domain of Tks5 binds to the N-terminus of XB130, whi

214 Finally, we demonstrate that the SH3 domain of UBASH3A mediates its binding to CBL-B, an

215 We demonstrate that the SH3 domains of CD2AP bind to PI3K and are necessary for

216 Tethering the SH3 domains of CD2AP or p110gamma to the membrane is suf

217 ogether, these results suggest that only the SH3 domains of CD2AP were necessary to enhance the E3 li

218 The SH3 domains of CD2AP were sufficient to drive the Cbl-3/

219 Despite these distinct roles, the SH3 domains of Cdc15 and Imp2 cooperate in the essential

220 These effects depend on the F-BAR and SH3 domains of CIP4 and on its ability to multimerize.

221 Here, we report crystal structures of tandem-SH3 domains of different STAC isoforms up to 1.2-A resol

222 SHP1 and THEMIS engage with the N-SH3 and C-SH3 domains of GRB2, respectively, a configuration that

223 edicted binding of the viral UL25 protein to SH3 domains of NCK Adaptor Protein-1.

224 f a Src family kinase inhibitor and when the SH3 domains of Nck are mutated.

225 The first and third SH3 domains of Nck are not required to recruit the WIP:N

226 the 50-residue linker between the first two SH3 domains of Nck enhances phase separation of Nck/N-WA

227 We identify the SH3 domains of nebulin and nebulette as novel ligands of

228 -Rak binding is direct, requires the SH2 and SH3 domains of Rak/Frk for efficient complex formation a

229 The SH3 domains of syndapin and endophilin bind the PXXP mot

230 report here that TbetaRI interacts with the SH3 domains of the adaptor protein CIN85 in response to

231 l as its complex with the ankyrin repeat and SH3 domains of the pro-apoptotic factor ASPP2.

232 ity, can be inhibited by the Src homology 3 (SH3) domain of a Ras-specific GAP (p120RasGAP).

233 expression and show that the Src-homology 3 (SH3) domain of ABL2 directly interacts with HSF1 protein

234 CD3e that binds to the first Src homology 3 (SH3) domain of Nck (Nck-SH3.1).

235 molecular recognition of the Src homology 3 (SH3) domain of the yeast protein Sho1 with its cognate p

236 e-rich protein, binds to the Src homology 3 (SH3) domains of GRB2 resulting in PI3K activation.

237 ne-rich region 1 of THEMIS to the C-terminal SH3-domain of GRB2.

238 catalytic domain or Src homology 2 (SH2) or SH3 domains or of the cysteine residue that undergoes LP

239 s crucial in the recognition between PRM and SH3 domain, our results suggest that attaching multiple

240 ovide additional specificity to the syndapin SH3 domain outside of the well described polyproline-bin

241 nc-89 loss-of-function mutants that lack the SH3 domain, paramyosin is found in accumulations.

242 ole of BAR sequence-mediated dimerization on SH3 domain partnership.

243 activities as well as a dramatic decrease of SH3 domain partnership.

244 We found that the ITK and LCK SH3 domains potentially have adjacent and overlapping bi

245 its Dyn2-binding C-terminal Src homology 3 (SH3) domain prevents Hsc70 binding, resulting in a stabi

246 les in establishment of a robust multivalent SH3 domain-PRM network in vivo, giving actin assembly on

247 rcoma) to a multivalent poly-Src homology 3 (SH3) domain protein that phase-separates when mixed with

248 suggesting that the spatial presentation of SH3 domains, rather than affinity, governs the recruitme

249 Thus, the data suggest that the SH3 domain recognizes its cognate peptide with a compone

250 o induce curvature; however, the role of the SH3 domain remains controversial.

251 igand interactions and cellular functions of SH3 domains requires detailed structural, atomic-resolut

252 ain responsible for membrane bending, and an SH3 domain responsible for the recruitment of dynamin an

253 d found that the peptide interacted with the SH3 domain RT-loop and surrounding residues.

254 t, iteratively with Y221, the Crk C-terminal SH3 domain (SH3C) is routinely phosphorylated on Y239 an

255 pY221 auto-clamp to interrupt SH2-N-terminal SH3 domain (SH3N) signaling.

256 nd that mutants that lack either H0 helix or SH3 domain show significantly faster dissociation kineti

257 ning two ADAP-binding SKAP55 Src homology 3 (SH3) domains stabilized SLP-76 microclusters and promote

258 rogen bonds (13.0 +/- 0.6 kcal/mol), and the SH3 domain stabilizes the structure of the N-terminal he

259 Cytosolic proteins containing SH2 and SH3 domains, such as Crk and Crk-like (CrkL), are broadl

260 ology 2 (SH2) domain and alphavbeta3 via its SH3 domain, suggesting the kinase links the two receptor

261 me cases causes dimerization of endophilin's SH3 domains, suggesting that the spatial presentation of

262 formation is dependent on the number of Nck SH3 domains, suggesting they are phase separated polymer

263 in the protein center, while the C-terminal SH3 domain tandem binds RIM, Munc13, and Ca2+ channels r

264 We find that different SH3 domains target distinct proline-rich sequences overl

265 The SH3 domain tends to generate homodimers through a domain

266 dc42- and SH3-binding CRIB-PR domain, and an SH3 domain that binds downstream cytoskeletal effectors.

267 The process involves a SH3 domain that binds to a region of the RecB subunit in

268 s applied to the folding reaction of the Fyn SH3 domain that exchanges between a highly populated, NM

269 domain downstream of the N-terminal SH2 and SH3 domains that regulate catalytic function and membran

270 daptor protein Nck has three SRC-homology 3 (SH3) domains that bind multiple proline-rich segments in

271 CD2AP contains three Src homology 3 (SH3) domains that mediate multiple protein-protein inter

272 We show, using simulations of src SH3 domain, that mechanical force enhances the populatio

273 a remarkable allosteric network linking the SH3 domain, the myristic acid binding pocket, and the ac

274 theless, an amino acid stretch preceding the SH3 domain, the so-called N-cap, reshapes the free-energ

275 ent surface uniformity and conserved fold of SH3 domains, they display different binding mechanisms a

276 nts the ability of p130(cas) to bind the Src SH3 domain through an RPLPSPP motif in the p130(cas) SBD

277 We predict that the helix binds to the SH3 domain through hydrophobic and salt-bridge interacti

278 s, we studied the binding properties of each SH3 domain to the known interactor Casitas B-lineage lym

279 es splicing to insert short sequences in the SH3 domain to yield N1- and N2-Src.

280 We applied the strategy to SH3 domains to determine the properties of their binding

281 inases, acts in conjunction with the SH2 and SH3 domains to stabilize the inactive conformation.

282 induced aggregation of membrane-targeted Nck SH3 domains to test these predictions and to determine h

283 g GTPase complex through its Src homology 3 (SH3) domain under conditions of amino acid starvation an

284 SH3 domains usually interact with a proline-rich consens

285 N-terminal deleted mutant and/or Tks5 fifth SH3 domain W1108A mutant.

286 residue) with the cortactin-Src homology 3 (SH3) domain was critical for maintaining the level of ac

287 hesis that it targets Src homology domain 3 (SH3) domains was tested, but mutation of the putative SH

288 Conformational states of the metastable drkN SH3 domain were characterized using single-molecule fluo

289 logy 2 domain, followed by a Src homology 3 (SH3) domain, whereas CrkII possesses in addition a C-ter

290 ized with aPKC, Dlg is phosphorylated in its SH3 domain which disrupts autoinhibition and allows GukH

291 TOCA1 was recruited to pedestals by its SH3 domain, which bound directly to proline-rich sequenc

292 hich binds the PP1 catalytic domain, and its SH3 domain, which engages the PP1 C-terminal tail.

293 n addition a C-terminal linker region plus a SH3 domain, which operate as regulatory moieties.

294 into a putative interaction mode of the p120 SH3 domain with the DLC1 RhoGAP domain that is atypical

295 Cocrystallization of the nebulette SH3 domain with the interacting XIRP2 peptide PPPTLPKPKL

296 ing motifs are mapped and shown to bind both SH3 domains with micromolar affinity.

297 tion, and whether it affects interactions of SH3 domains with other cellular ligands, remain unclear.

298 nds the amphiphysin-2 (BIN1) Src homology-3 (SH3) domain with an unusually high affinity (Kd 24 nm).

299 the specific binding of the Src homology 3 (SH3) domain with short proline-rich peptides.

300 in metazoans as identified by an N-terminal SH3 domain, YxxP motifs, and a C-terminal FAT-like domai