ライフサイエンスコーパス: SHG

1 SHG angular intensity pattern (SHG-AIP) of healthy and p

2 SHG confocal imaging of collagen fibres revealed a reduc

3 SHG endomicroscopic imaging of ex vivo murine and human

4 SHG microscopy is an emerging microscopic technique for

5 SHG results show that interfacial polarity probed by p-n

6 SHG spectra confirm that the interface is, in fact, pola

7 SHG spectra report the electronic structure of solutes a

8 SHG together with the Poisson-Boltzmann equation yielded

9 SHG-AIP with two symmetrical spots is found to be a sign

10 SHG-based three-dimensional reconstructions of Chlamydom

11 Additionally, SHG spectra show a strong resonance at long wavelengths

12 h multiphoton bandpass filters to obtain AF, SHG (collagen bandwidth), and eosin-labeled fluorescence

13 r-simultaneous image acquisition pairs of AF-SHG (+/-eosin coincubation), AF-eosin, and SHG-eosin wer

14 l meshwork for image acquisition pairs of AF-SHG (without eosin coincubation) and SHG-eosin.

15 ral SRS and SHG imaging reveals that not all SHG-active structures with solidlike morphologies can be

16 a universal 3-nitropyrolle nucleotide as an SHG-active label, we monitored the hybridization rate an

17 d truly non-invasive nature of live CARS and SHG imaging and their value and translation potential in

18 in three-dimensional cultures using CARS and SHG microscopy and demonstrate the live-imaging of the s

19 CARS and SHG/TPF imaging was performed at one wavenumber on the P

20 s of AF-SHG (without eosin coincubation) and SHG-eosin.

21 F-SHG (+/-eosin coincubation), AF-eosin, and SHG-eosin were captured.

22 However, combined hyperspectral SRS and SHG imaging reveals that not all SHG-active structures w

23 y exemplifies the merit of combining SRS and SHG microscopy for an enhanced label-free chemical analy

24 find that all of the reported materials are SHG-active at 1064 nm, with responses ranging from 2.8 t

25 As SHG offers a powerful, high-throughput screening approac

26 ssert that complex optical artifacts such as SHG verniers should be taken into account when applying

27 changes in fibrosis from the backward SHG (B(SHG)) alone, as only backward-propagating SHG is accessi

28 easure changes in fibrosis from the backward SHG (B(SHG)) alone, as only backward-propagating SHG is

29 eas, fSHG and bSHG (forward-SHG and backward-SHG) collagen parameters achieved their lowest values in

30 SHG vernier" patterns, regions of bifurcated SHG intensity, are illusory when sarcomeres are staggere

31 The high spatial resolution afforded by SHG microscopy allows for the use of a minibeam collimat

32 field imaging, followed by classification by SHG.

33 static electric fields easily detectable by SHG.

34 y important as collagen deposition imaged by SHG, remain poorly exploited to date.

35 at can also be detected at the bond level by SHG.

36 t agreement with the classifications made by SHG, with measurement times of approximately 1 min and s

37 , and anticocaine antibodies was measured by SHG, allowing binding affinities and rates of dissociati

38 /desolvation events were readily observed by SHG imaging and directly correlated to the phase transfo

39 ntrosymmetric crystal forms were observed by SHG microscopy.

40 tted to evaluation of collagen parameters by SHG.

41 es outside of the interfacial zone probed by SHG.

42 DCNNs based on CARS, SHG/TPF, or Raman images have discriminated between norm

43 DCNNs) were trained independently with CARS, SHG/TPF, and Raman images, taking into account both morp

44 ferentiation and tissue engineering, if CARS/SHG microscopy is to be used as a non-invasive, label-fr

45 These results demonstrate that CARS/SHG/TPF microscopy has a prospective use as a label-free

46 In both cases, SHG was able to resolve conformational changes in these

47 the mouse eye was also visible, with a clear SHG signal representing collagen fibers.

48 lso provide details for injecting PEG-coated SHG nanoprobes into zygote-stage zebrafish embryos, and

49 The glass of APSe(6) exhibits comparable SHG intensities to the top infrared NLO material AgGaSe(

50 sitional analysis reveal that the cumulative SHG intensity within each image volume and the average c

51 direct fitting of the polarization dependent SHG signal.

52 Phasor projection of Polarization-dependent SHG (muMAPPS) that maps the features of the collagen arc

53 Polarization-dependent SHG measurement and synchrotron X-ray microdiffraction a

54 erimental characterization of myosin-derived SHG intensity profiles within intact zebrafish skeletal

55 tly spatially ordered to generate detectable SHG without the use of any fluorescent dye.

56 sin; allowing SHIM to characterize different SHG-generating components within a complex biological sa

57 evements in the field of frequency doubling (SHG) and tripling (third-harmonic generation, THG) in th

58 delivery, scanning, focusing, and efficient SHG signal collection.

59 ield-induced second harmonic generation (EFI-SHG) technique that can directly monitor the dynamic per

60 The EFI-SHG studies confirmed the motion of the water can remove

61 After the contact, the EFI-SHG system visualized briefly three relaxations of the t

62 xibility and material selectivity of the EFI-SHG.

63 Endomicroscopic SHG images of murine cervical tissue sections at differe

64 t chi(3) experiments and resonantly enhanced SHG experiments that probe the ligand-to-metal charge tr

65 e ability of malachite green as an excellent SHG-indicator of changes of individual cell membrane and

66 The as-prepared glass fibers exhibit SHG and difference frequency generation (DFG) responses

67 ing methods (including organic fluorophores, SHG chromophores, genetic indicators, hybrid, nanopartic

68 rp contrast with most materials designed for SHG purposes, which generally require the use of expensi

69 4, and all-trans-retinol, were evaluated for SHG effectiveness in Jurkat cells.

70 ifically, the measured scattering length for SHG was in excellent agreement with the value predicted

71 ssion based on an electrooptic mechanism for SHG, which depends on the complex-valued first- and seco

72 However, in the case of forward SHG, although the same changes impact upon absolute inte

73 n intratumoral areas, fSHG and bSHG (forward-SHG and backward-SHG) collagen parameters achieved their

74 wing confirmation of largely background-free SHG imaging of common crop leaves (soybean, maize, wheat

75 The agreement between Ka values derived from SHG measurements of the interactions between SERMs and a

76 taken into account when applying functional SHG imaging as a diagnostic readout for pathological mus

77 agen and myosin by ratiometric epi-generated SHG images at 920 nm and 860 nm.

78 difference between forward and epi-generated SHG provides an explanation for many of the wavelength-d

79 op a model for the forward and epi-generated SHG wavelength-dependence.

80 n the wavelength-dependency of epi-generated SHG.

81 Backscattered second harmonic-generated (SHG) NLO signals from these sections were collected as a

82 nfocal imaging of second harmonic-generated (SHG) signals can detect corneal collagen organization.

83 demonstrate that second harmonic generating (SHG) nanoprobes can be used for in vivo imaging, circumv

84 ently introduced second harmonic generating (SHG) nanoprobes.

85 Bright second harmonic generation (SHG) (up to 18 million counts/s) was observed from even

86 tric material is second harmonic generation (SHG) active at both 1064 and 532 nm, with efficiencies o

87 s labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers.

88 c material shows second-harmonic generation (SHG) activity at both 1064 and 532 nm with efficiencies

89 for quantifying second harmonic generation (SHG) activity of powders that largely decouples linear a

90 each detector in second harmonic generation (SHG) and three parameters for the transmittance of the i

91 myocardium using second harmonic generation (SHG) and two photon excited autofluorescence.

92 dalities such as second-harmonic generation (SHG) and two-photon excited fluorescence (TPEF) have onl

93 uct simultaneous second harmonic generation (SHG) and two-photon fluorescence measurements on two dif

94 ering (CARS) and second harmonic generation (SHG) are non-linear techniques that allow label-free, no

95 ) exhibits second-order harmonic generation (SHG) at both 1064 and 532 nm incident radiation with eff

96 Second-harmonic generation (SHG) by membrane-incorporated probes is a nonlinear opti

97 cence (TPEF) and second harmonic generation (SHG) can image the endogenous signals of tissue structur

98 , a custom-built second harmonic generation (SHG) confocal microscope was used to study dynamic sarco

99 n is paid to the second harmonic generation (SHG) effect.

100 violet by direct second harmonic generation (SHG) enabled by a new beryllium-free zincoborate-phospha

101 of epi-generated second harmonic generation (SHG) excitation efficiency, and discriminate collagen an

102 ated by means of second harmonic generation (SHG) experiments and simulations.

103 Initial second harmonic generation (SHG) experiments showed crystalline K(4)GeP(4)Se(12) out

104 characterize AF, second harmonic generation (SHG) for collagen, and eosin-labeled fluorescence identi

105 cence (TPEF) and second-harmonic generation (SHG) from biological and inorganic media.

106 oassay utilizing second harmonic generation (SHG) has been developed and the utility of the method ha

107 sonance enhanced second harmonic generation (SHG) has been used to identify solvation mechanisms at d

108 Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences

109 high-resolution second harmonic generation (SHG) imaging of biological tissues and demonstrate its u

110 y integration of second harmonic generation (SHG) imaging with differential scanning calorimetry (DSC

111 hich is based on second-harmonic generation (SHG) imaging, enabled detection of homochiral microcryst

112 fluorescence and second harmonic generation (SHG) imaging.

113 Second Harmonic Generation (SHG) is a label-free imaging method used to monitor coll

114 Second harmonic generation (SHG) is an emergent biophysical method that sensitively

115 Second harmonic generation (SHG) is an inherently surface specific technique making

116 mpounds includes second harmonic generation (SHG) measurements, theoretical calculations, infrared an

117 Second harmonic generation (SHG) microendoscopy is an emerging technology for imagin

118 A low-cost second harmonic generation (SHG) microscope was constructed, and, for the first time

119 m a Mueller to a Second Harmonic Generation (SHG) microscope, providing a pixel-to-pixel matching of

120 ere imaged using second-harmonic generation (SHG) microscopy for both collagen and elastin.

121 Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for i

122 y observed in 3D Second Harmonic Generation (SHG) microscopy image data of normal (1) and high risk (

123 Second-harmonic generation (SHG) microscopy is a valuable imaging technique to probe

124 Second harmonic generation (SHG) microscopy measurements indicate that inkjet-printe

125 py combined with second-harmonic generation (SHG) microscopy to selectively detect ChC.

126 interferometric second harmonic generation (SHG) microscopy with femtosecond pulses.

127 rast provided by second harmonic generation (SHG) microscopy, it is possible to identify early molecu

128 trate the use of second harmonic generation (SHG) microscopy-guided synchrotron powder X-ray diffract

129 visualizable by second harmonic generation (SHG) microscopy.

130 Red staining and Second Harmonic Generation (SHG) Microscopy.

131 can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm

132 The second harmonic generation (SHG) of vertical and planar split-ring resonators (SRRs)

133 Second-harmonic generation (SHG) originates from the interaction between upconverted

134 luminescence and second-harmonic generation (SHG) phenomena; these have been covered in numerous prev

135 imaging based on second harmonic generation (SHG) provides rapid and highly selective detection of in

136 , exhibit strong second harmonic generation (SHG) response in both crystal and glassy forms.

137 ar optical (NLO) second harmonic generation (SHG) response in the wavelength range of 600-950 nm.

138 exhibits a large second harmonic generation (SHG) response of 13.5 x KDP (600 x alpha-SiO2), and the

139 ay a spontaneous second harmonic generation (SHG) response without any need for preprocessing, and th

140 ng to their weak second harmonic generation (SHG) response.

141 ar optical (NLO) second harmonic generation (SHG) response.

142 the creation of second harmonic generation (SHG) signals makes it an attractive technique for visual

143 ve in generating second harmonic generation (SHG) signals when adsorbed onto surfaces of colloidal mi

144 sonance-enhanced second harmonic generation (SHG) spectra were collected from 4-dimethylaminobenzonit

145 ization-resolved second harmonic generation (SHG) spectroscopy at the single-particle level.

146 mental thin-film second harmonic generation (SHG) spectroscopy confirms these trends in calculated re

147 cells, we apply second harmonic generation (SHG) spectroscopy using SHG-active antimicrobial compoun

148 ribe resonant UV second harmonic generation (SHG) studies of the strongly chaotropic thiocyanate ion

149 A second-harmonic generation (SHG) study revealed Kleinman symmetry-forbidden nonlinea

150 ence (TPEF), and second-harmonic generation (SHG) to investigate the consequences of early septic liv

151 ng time-resolved second-harmonic generation (SHG) to study a ferroelectric (FE)/ferromagnet (FM) oxid

152 d measurement by second-harmonic generation (SHG) together with the anisotropic-bond model of nonline

153 ring (QCM-D) and second harmonic generation (SHG) using solid-supported lipopolysaccharide-containing

154 Second harmonic generation (SHG) was integrated with Raman spectroscopy for the anal

155 In this study, second-harmonic generation (SHG) was used to study RNA and DNA oligonucleotide confo

156 SONICC relies on second harmonic generation (SHG), a nonlinear optical effect that only arises from n

157 n microscopy and second harmonic generation (SHG), and there are advantages over confocal microscopy

158 = 1.369 mum) for second harmonic generation (SHG), the largest NLO susceptibility reported to date fo

159 cence (2PAF) and second harmonic generation (SHG), were used to obtain images of the trabecular meshw

160 ential sensitive second harmonic generation (SHG), which is a label-free and interface-selective meth

161 ree technique of second harmonic generation (SHG).

162 e was studied by second harmonic generation (SHG).

163 by measuring the second harmonic generation (SHG).

164 The observed second-harmonic generation (SHG; a nonlinear optical process that converts two photo

165 fluorescence and second-harmonic-generation (SHG) imaging.

166 (TPE) and Second Harmonic signal Generation (SHG).

167 This study highlights SHG as a high-throughput screening platform that reveals

168 ed graphitic sheets with stereoscopic holes (SHG) act as effective tri-functional catalysts for the o

169 We discuss how SHG can be used to obtain more structural information on

170 we observed an instantaneous drop of ~50% in SHG signal from the anodic pole of the cell.

171 While image analysis of collagen in SHG images was consistently addressed until now, cellula

172 e aim to take advantage of the difference in SHG between aligned and partially aligned molecules to p

173 We hypothesize that this loss in SHG signal is due to disruption in the interfacial natur

174 g of collagen fibres revealed a reduction in SHG intensity after CTS, with regions of disorganisation

175 ons and larger M cations result in increased SHG efficiencies.

176 2-keV (1.033-A) X-rays resulted in increased SHG in the region extending approximately 3 mum beyond t

177 Collagen shows increased SHG intensity at 920 nm, while little difference is dete

178 indicating a possible electric-field-induced SHG (EFISH) mechanism for generating the observed signal

179 tude larger than known magnetization-induced SHG(8-11) and comparable to the SHG of the best (in term

180 The X-ray-induced SHG activity was observed with no measurable loss for lo

181 Finally, an examination of the known SHG active AMCO3F (A = alkali metal, M = alkaline earth

182 V absorption edge, below 190 nm, and a large SHG response, 2.8xKDP.

183 he C-site is critical for generating a large SHG response.

184 Ba3(ZnB5O10)PO4, exhibits large SHG responses at 1064 and 532 nm and a short 180 nm abso

185 is article, we explore the use of lipophilic SHG probes to detect minute perturbations in the plasma

186 isson-Boltzmann equation to the TAT liposome SHG data, was shown to be in good agreement with an appr

187 nized noncentrosymmetric structure emits low SHG signal intensity if it has no predominant local pola

188 K(4)GeP(4)Se(12) also exhibits a measurable SHG response with no poling.

189 s the second-harmonic generation microscopy (SHG).

190 maged by conventional two-photon microscopy, SHG nanoprobes appear to generate a signal with an inver

191 SHG signal, the observed giant nonreciprocal SHG originates only from the layered antiferromagnetic o

192 e also show that ChCs exhibit a nonvanishing SHG signal, corroborating the noncentrosymmetry of the c

193 low colocalized with SHG (Mcoeff=1), but not SHG signal-voids.

194 effects, collectively that form the observed SHG contrast.

195 te of the art and the physical background of SHG microscopy, and then describe the optical modificati

196 e nanoscale governs the coherent building of SHG signal within the focal volume and is a key advance

197 rtifacts arise due to the phase coherence of SHG signal generation and the Guoy phase shift of the la

198 The voltage dependence of SHG by four different probes, three retinoids (all-trans

199 The resulting contrast and detectability of SHG nanoprobes provide unique advantages for molecular i

200 concept, we imaged the phase distribution of SHG signal from the complex collagen architecture of juv

201 ental results show a 2.6-fold enhancement of SHG nonlinearity, which is in good agreement with simula

202 mplexity and often inefficient excitation of SHG and TPEF signals.

203 Our findings demonstrate the feasibility of SHG endomicroscopy technology for staging normal pregnan

204 acent to the iris and cornea that is free of SHG signal and consistent with the location of Schlemm's

205 ge zebrafish embryos, and in vivo imaging of SHG nanoprobes during gastrulation and segmentation.

206 Imaging of SHG signals provides a sensitive means for detection of

207 y describes the successful implementation of SHG as a primary screening platform to identify fragment

208 The detection limit of SHG was estimated to be 4 ppm crystallinity in the powde

209 Moreover, the maximum of SHG intensity is observed at slightly off-resonance exci

210 Making use of the coherent nature of SHG, we monitored in real-time the transfer of 2 and 3 b

211 te understanding of the structural origin of SHG signals from tissues.

212 Our work demonstrates the broad potential of SHG for studying oligonucleotides and their conformation

213 cteria while also demonstrating the power of SHG to examine these dynamics.

214 Linear scaling of SHG response with film thickness is observed for benzoic

215 The unique sensitivity of SHG furthered our study by revealing distinct conformati

216 bution of second-order nonlinear emitters on SHG-AIP is highlighted.

217 These surface-bound and oriented SHG-active MOFs have the potential for use as single non

218 tion-dependent second-harmonic generation (P-SHG) microscopy.

219 From the P-SHG parameters of vertebrae tissue, a three-dimensional

220 for the preparation and use of a particular SHG nanoprobe label, barium titanate (BT), for in vivo i

221 SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are s

222 nt magnets, chiral magnets and photomagnets, SHG-active magnetic materials, pyro- and ferroelectrics,

223 goal, we implemented a circular polarization SHG imaging approach and used it to quantify the intensi

224 Unlike commonly used fluorescent probes, SHG nanoprobes neither bleach nor blink, and the signal

225 s revealed by various validation procedures, SHG signal intensities are a reliable relative measure o

226 (B(SHG)) alone, as only backward-propagating SHG is accessible for true in vivo applications.

227 Quantitative SHG microscopy enabled discrimination of crystal form on

228 This renders SHG microscopy highly sensitive to molecular distributio

229 A comparison with polarization-resolved SHG highlights the crucial role of relative fibril polar

230 ntal implementation of polarization-resolved SHG microscopy combined with mechanical assays, to simul

231 nce that anisotropy of polarization-resolved SHG mostly reflects the micrometer-scale disorder in the

232 4 M and higher concentrations, the resonant SHG signal increases discontinuously, indicating a struc

233 o the structural state of muscle sarcomeres, SHG functional imaging can give insight into the integri

234 nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while

235 ransparency window and achieving the sizable SHG response.

236 ds before being converted to the more stable SHG-inactive polymorphic forms.

237 These can be performed in any standard SHG-microscope that allows polarization control of the i

238 h- and low-intensity regions in the standard SHG images.

239 that gamma-NaAsSe(2) has the highest static SHG coefficient known to date, 337.9 pm/V, among materia

240 In addition to a strong SHG activity, the trimer also shows a strong third harmo

241 (250 nm) of reported materials with a strong SHG response (>10 x KDP).

242 Di-4 proved superior with both strong SHG signal and limited bleaching artifacts.

243 metastructures and vertical SRRs with strong SHG nonlinearity majorly result from magnetic dipole and

244 bit is up to approximately 15 times stronger SHG than that of commercially used AgGaSe(2).

245 rodynamic simulations which demonstrate that SHG is also determined by the local field distribution i

246 Given that SHG signal is sensitive to the structural state of muscl

247 Our results indicate that SHG is a highly sensitive probe of subtle magnetic order

248 These results indicate that SHG microendoscopy provides a means for developing a qua

249 Our results show that SHG combined with molecular design and synthesis of surf

250 The results show that SHG imaging has great potential as a tool for measuring

251 Our results show that SHG not only can monitor the movement of small molecules

252 We also show that SHG-AIP provides information on the three-dimensional st

253 We determined that "SHG vernier" patterns, regions of bifurcated SHG intensi

254 The SHG activity of a crystal is highly sensitive to the spe

255 The SHG intensity and forward-backward ratios decrease with

256 The SHG intensity is the largest for silicates without secon

257 The SHG primary immunoassay has provided the first kinetic m

258 ble substrate affixed to a stretcher and the SHG live-cell imaging technique are unique tools for rea

259 Collectively, the SHG measurements and analysis all indicate that incorpor

260 relative fibril polarity in determining the SHG signal intensity.

261 oped the pixel-based approach to extract the SHG signal polarization anisotropy from the same polariz

262 However, the SHG measurements demonstrated three decade improvements

263 We observe a decrease in the SHG anisotropy parameter when the tendon is stretched in

264 gen and glucose deprivation, a change in the SHG response to the polarization was measured.

265 structures, we show that the changes in the SHG signals upon ligand binding are the result of struct

266 It is the SHG sensitivity to the electrostatic field generated by

267 resonances in the bow-tie nanoantennas, the SHG signal is enhanced; this despite the fact that the l

268 ith SHG signal-voids (Mcoeff=1), but not the SHG signal.

269 Mcoeff=1) with SHG signal-voids, but not the SHG signal.

270 , these data illustrate the potential of the SHG approach for detecting and measuring protein conform

271 Monte Carlo simulations of the SHG axial directional and attenuation responses allow th

272 , this approach allows for estimation of the SHG creation attributes (directionality and relative con

273 timate that the setup and calibration of the SHG instrument from its component parts will require 2-4

274 Additionally, histograms of the SHG intensities as functions of particle size and orient

275 Functionalization of the SHG nanoprobes takes approximately 3 d, whereas zebrafis

276 by measuring the intensity modulation of the SHG signal as a function of the angular rotation of the

277 demonstrate that different magnitudes of the SHG signal changes are due to different and specific lig

278 amorphous vitreous solvents, analysis of the SHG spatial profiles following X-ray microbeam exposure

279 NLO-active structural units in producing the SHG responses.

280 tion-induced SHG(8-11) and comparable to the SHG of the best (in terms of nonlinear susceptibility) t

281 mmetric, and thus does not contribute to the SHG signal, the observed giant nonreciprocal SHG origina

282 l's resting potential, the voltage where the SHG is minimal, and the amplitude of the signal at that

283 focus of a laser-scanning microscope, these SHG nanocrystals convert two photons into one photon of

284 without any need for preprocessing, and this SHG activity appears to be stable over several months.

285 pe was constructed, and, for the first time, SHG microscopy was used for imaging agrochemical materia

286 n the other hand can potentially be added to SHG and TPEF to visualize a much broader range of marker

287 ic mice and sham-treated mice in contrast to SHG (AUC = 0.49).

288 with resolution similar to that of bench-top SHG microscopy.

289 ect of the N, S heteroatom doping and unique SHG architecture, which provide a large surface area and

290 Using SHG, we identified a fragment binder to KRas(G12D) and u

291 Using SHG, we quantify periplasmic and cytoplasmic accumulatio

292 in membrane symmetry could be detected using SHG, we exposed cells to nanosecond-pulsed electric fiel

293 ively probe the ferroelectric response using SHG.

294 harmonic generation (SHG) spectroscopy using SHG-active antimicrobial compound malachite green as the

295 on crop leaves (soybean, maize, wheatgrass), SHG microscopy was used to image active ingredient cryst

296 to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscl

297 d quantitatively colocalized (Mcoeff=1) with SHG signal-voids, but not the SHG signal.

298 and quantitatively, AF-low colocalized with SHG (Mcoeff=1), but not SHG signal-voids.

299 AF-high colocalized with SHG signal-voids (Mcoeff=1), but not the SHG signal.

300 ne materials are type-I phase-matchable with SHG coefficients chi((2)) of 151.3 and 149.4 pm V(-1) fo