ライフサイエンスコーパス: SICM

1 SICM can be used to analyze cell morphology at nanoscale

2 SICM employs a nanopipette tip that contains electrolyte

3 SICM enhancements have enabled surface charge detection

4 SICM has traditionally been a topography-mapping microsc

5 SICM is complemented with live-cell compatible super-res

6 SICM measurements, fitted to a simplified finite element

7 SICM results find heterogeneities across the bacterial s

8 SICM was also able to detect regions of high negative ch

9 The spatial resolution of AC-SICM is an order of magnitude larger than the tip size (

10 In addition, SICM has been integrated with a range of other technique

11 d carbon electrode for pH measurement and an SICM barrel for distance control, enabling simultaneous

12 etermining the tip-sample distance during an SICM experiment.

13 ged electroactive molecules (solute) from an SICM tip to a working electrode substrate to determine t

14 . C 20.60+/- 3.174x10(5), n=56; P<0.001) and SICM studies revealed a profound disruption to the openi

15 The fabricated probes, with pH and SICM sensing elements typically on the 100 nm scale, wer

16 the relation between its key properties and SICM currents, building foundations to further investiga

17 Duplex patch clamping and Angle SICM recordings show that I(Na) and I(KATP) functionally

18 our new, to our knowledge, angular approach SICM allows imaging of living cells on nontransparent su

19 Using this angular approach SICM, we obtained topographical images of cells grown on

20 ue to the limitations of currently available SICM systems that inherited their design from other scan

21 red scanning ion conductance microscopy (bio-SICM) approach that couples the imaging ability of SICM

22 urther optimization, we believe that the bio-SICM platform will provide a powerful analytical methodo

23 To establish the framework of the bio-SICM platform, we utilize the well-studied protein chann

24 Bias modulated (BM)-SICM is compared to conventional SICM imaging through me

25 Finally, BM-SICM with both amplitude and phase feedback is used for

26 roscopy and faradaic electrodes have brought SICM to the forefront as a tool for nanoscale chemical m

27 omyocytes at many locations across a cell by SICM and synchronize these data using the simultaneously

28 ally termed 'sepsis-induced cardiomyopathy' (SICM), is common and has long been a subject of interest

29 By combining SICM data with finite element method (FEM) simulations t

30 In conclusion, SICM, unlike other techniques, can reliably deliver prec

31 ted ultramicroelectrode (UME) for concurrent SICM and scanning electrochemical microscopy (SECM).

32 In contrast, SICM enables quantitative surface charge mapping that sh

33 feedback signal, as needed for conventional SICM modes.

34 ulated (BM)-SICM is compared to conventional SICM imaging through measurements of substrates with dis

35 Correlative SICM-FCM reveals changes in morphology and kinetics of e

36 Finally, we employ correlative SICM/SOFI microscopy for visualizing actin dynamics in l

37 rates direct-current and alternating-current SICM imaging modes on polydimethylsiloxane (PDMS) struct

38 Despite the lack of a consensus definition, SICM is widely recognized as a reversible condition char

39 ferential concentration mode of SICM (DeltaC-SICM) also enhances surface charge measurements and prov

40 ce on the nanopipette response in the DeltaC-SICM configuration compared to standard SICM modes.

41 we demonstrate a new method for determining SICM tip geometry that overcomes the limitations of EM i

42 Finally, SICM is able to detect differences in the surface charge

43 For SICM imaging, the assumption is made that positions of e

44 electrode as part of a conductance cell for SICM.

45 ds significantly increased opportunities for SICM beyond recording topography.

46 Results from SICM were used to interpret heterogeneous reactivity stu

47 However, SICM imaging remains insensitive to electrochemical prop

48 we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimally invasive surfa

49 tify surface charge density and show that HP-SICM cannot be quantitatively described by a steady-stat

50 of mass transport and nanoscale delivery in SICM and a new means of synchronously mapping electrode

51 ative understanding of molecular delivery in SICM.

52 y for substantially better quantification in SICM imaging and measurement.

53 s provides new insights on mass transport in SICM that will enhance quantitative applications and ena

54 The results show that MEA-SICM provides an ultrafast imaging platform for investig

55 We use the MEA-SICM setup to visualize the effect of blebbistatin, a my

56 e of electroosmotic flow (EOF) in mechanical SICM measurements.

57 ip of a scanning ion conductance microscope (SICM) and a conductive (working electrode) substrate (tw

58 et in a scanning ion conductance microscope (SICM) can exert localized forces on a sample surface.

59 coupled scanning ion-conductance microscope (SICM) integrated with TERS for near-field spectroscopy u

60 The scanning ion conductance microscope (SICM) is a powerful tool for imaging the topography of s

61 The scanning ion conductance microscope (SICM) is an emerging imaging technique for the investiga

62 The scanning ion conductance microscope (SICM) is an emerging tool for noncontact topography imag

63 both a scanning ion conductance microscope (SICM) probe and an injection probe.

64 own the scanning ion conductance microscope (SICM) to be a very promising tool to spatially resolve a

65 ectrode scanning ion conductance microscope (SICM).

66 elative scanning ion conductance microscopy (SICM) and fluorescence confocal microscopy (FCM) to stud

67 ng with scanning ion conductance microscopy (SICM) and other scanning probe microscopies.

68 taneous scanning ion conductance microscopy (SICM) and scanning electrochemical microscopy (SECM) mea

69 ased on scanning ion conductance microscopy (SICM) and, as a proof of concept, was applied to longitu

70 bes for scanning ion conductance microscopy (SICM) as a robust platform for collecting spatial inform

71 present scanning ion-conductance microscopy (SICM) as an alternative approach for topographical imagi

72 olution scanning ion conductance microscopy (SICM) extends the utility of SICM by enabling selective

73 using a scanning ion conductance microscopy (SICM) format.

74 Scanning ion conductance microscopy (SICM) has developed into a powerful tool for imaging a r

75 Scanning ion conductance microscopy (SICM) has emerged as a versatile tool for studies of int

76 Scanning ion conductance microscopy (SICM) is a nanopipette-based scanning probe microscopy t

77 Scanning ion conductance microscopy (SICM) is a powerful and versatile technique that allows

78 Scanning ion conductance microscopy (SICM) is a powerful technique for imaging the topography

79 Scanning ion conductance microscopy (SICM) is a scanned probe microscopy technique in which t

80 Scanning ion conductance microscopy (SICM) is a scanning probe technique that allows investig

81 Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that

82 Scanning ion conductance microscopy (SICM) is a topographic imaging technique capable of prob

83 Scanning ion conductance microscopy (SICM) is becoming a powerful multifunctional tool for pr

84 Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for qua

85 s work, scanning ion conductance microscopy (SICM) is used for the first time to visualize the surfac

86 nvasive scanning ion conductance microscopy (SICM) of cells and which must be overcome in order to fo

87 Scanning ion conductance microscopy (SICM) offers the ability to obtain very high-resolution

88 Scanning ion conductance microscopy (SICM) offers the ability to perform contact-free, high-r

89 tion pH-scanning ion conductance microscopy (SICM) probes is reported.

90 a novel scanning ion conductance microscopy (SICM) technique for assessing the volume of living cells

91 used in scanning ion conductance microscopy (SICM) to determine, in a noncontact manner, the topograp

92 use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge env

93 ed with scanning ion conductance microscopy (SICM) to show that functional beta(3)-ARs are mostly con

94 Scanning ion conductance microscopy (SICM) was used to interrogate ion currents emanating fro

95 combine scanning ion conductance microscopy (SICM) with a microelectrode array (MEA) to image the thr

96 With scanning ion conductance microscopy (SICM), a noncontact scanning probe technique, it is poss

97 e using scanning ion conductance microscopy (SICM), a scanning nanopipette probe technique that uses

98 (SECM), scanning ion conductance microscopy (SICM), electrochemical scanning tunneling microscopy (EC

99 py, and scanning ion conductance microscopy (SICM).

100 Here, we show how the hopping mode SICM method (HP-SICM) can be used for rapid and minimall

101 Using distance-modulated SICM, which induces an alternating ion current component

102 urthermore, the use of a distance modulation SICM scheme allows reasonably faithful probe positioning

103 we describe the designs that allow mounting SICM scan head on a standard patch-clamp micromanipulato

104 approach that couples the imaging ability of SICM with the sensitivity and chemical selectivity of pr

105 One of the great advantages of SICM lies in its ability to perform contact-free scannin

106 The application of SICM reaction imaging is demonstrated on several example

107 y also provide a framework for the design of SICM experiments, which may be convoluted by topographic

108 this review, we chronicle the development of SICM from the perspective of both the development of ins

109 ontributes significantly to the emergence of SICM as a multifunctional technique for simultaneously p

110 Hybridization of SICM with coincident characterization techniques such as

111 This differential concentration mode of SICM (DeltaC-SICM) also enhances surface charge measurem

112 k for understanding the contact-free mode of SICM and also extend previous findings with regard to SI

113 lustrates the feasibility and performance of SICM-TERS as a versatile tool for label-free nanoscale s

114 Despite the increasing popularity of SICM, several aspects of the imaging process are still u

115 pathophysiology and clinical presentation of SICM, and to provide insights to aid clinicians in manag

116 this report, we develop the use and scope of SICM, showing how it can be used for mapping spatial dis

117 been undertaken to enhance understanding of SICM as an electrochemical cell and to enable the interp

118 and provides improved insights on the use of SICM for controlled delivery to interfaces generally.

119 nce microscopy (SICM) extends the utility of SICM by enabling selective chemical imaging of specific

120 annel capillary probes consisting of an open SICM barrel, and a solid carbon SECM electrode enabled c

121 Collectively, our SICM-FCM findings at single CCP level, backed up by elec

122 P-SICM shows the ability to differentiate transport throug

123 ies conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent

124 We describe P-SICM investigations of both wild type and tricellulin ov

125 etric-scanning ion conductance microscopy (P-SICM) for ion-conductance measurement in polymer membran

126 etric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate

127 etric scanning ion conductance microscopy (P-SICM).

128 e, we combine hopping mode techniques with P-SICM to allow simultaneous nanometer-scale conductance a

129 em illustrates the quantitative nature of pH-SICM imaging, because the dissolution process changes th

130 The capability of the pH-SICM probe was demonstrated by detecting both pH and top

131 These pH-SICM probes were fabricated rapidly from laser pulled th

132 and it provides a framework for quantitative SICM studies.

133 sign elements include a full sample-scanning SICM, optics for TERS, and noise-isolating methods.

134 The suitability of the probes for SECM-SICM imaging is demonstrated by both feedback-mode and s

135 cal-scanning ion conductance microcopy (SECM-SICM) has been used to map the electroactivity of surfac

136 monstrates the value of high-resolution SECM-SICM for low-current amperometric imaging of nanosystems

137 This probe geometry enables successful SECM-SICM imaging on features as small as 180 nm in size.

138 We show that the EOF in small SICM nanopipettes is comparable to the flow induced by c

139 ltaC-SICM configuration compared to standard SICM modes.

140 erfaces, this work further demonstrates that SICM should generally become an important characterizati

141 The SICM is used to position the nanopipette above the cell

142 The SICM method can also be used for rapid estimation of the

143 The SICM-TERS system demonstrates direct-current and alterna

144 si-reference counter electrode (QRCE) in the SICM nanopipet probe and a second QRCE in the bulk solut

145 si-reference counter electrode (QRCE) in the SICM nanopipette probe and a similar electrode in bulk s

146 btilis cell wall structure can influence the SICM current response, a more comprehensive FEM model, a

147 Through control of the SICM bias applied between a quasi-reference counter elec

148 molecules highly tunable via control of the SICM bias to promote or restrict migration from the pipe

149 The heart of the SICM is a nanometer-scale electrolyte filled glass pipet

150 n is highly dependent on the geometry of the SICM probe, which is generally not known.

151 ts have allowed a critical assessment of the SICM response as a means of probing surface topography.

152 l experimental practice, the response of the SICM tip to surface features occurs over much greater la

153 Despite the rising popularity of the SICM, the image formation process and the fundamental li

154 ngs by changing the applied voltage over the SICM nanopipette.

155 The data can be routinely obtained using the SICM apparatus itself and our method thus opens the way

156 antitatively probe sample stiffness with the SICM.

157 In recent years, the SICM has been increasingly applied to mechanical measure

158 Practical implementations of these SICM advantages, however, are often complicated due to t

159 Modern approaches to SICM realize an important tool in analytical, bioanalyti

160 also extend previous findings with regard to SICM resolution.

161 nstrate the utility of this understanding to SICM by topographically mapping a live cell's cytoskelet

162 subtilis, not detected in the topographical SICM response and attributed to the extracellular polyme

163 Using SICM-FCM, which we have developed, we show how p.R465W m

164 topography scans of the cardiomyocytes using SICM and then determined the electrophoretic mobility of

165 le transport properties can be measured with SICM.

166 r mapping surface charge and topography with SICM, which increases the data acquisition rate by an or