ライフサイエンスコーパス: T-DNA

1 T-DNA and ALC loci were not linked, as indicated by rand

2 T-DNA inactivation of both Tric proteins further resulte

3 T-DNA insertion lines for ZML2 and its homolog ZML1 demo

4 T-DNA insertion mutants (atg mutants) of these genes dis

5 T-DNA insertion mutants are a tool used widely in Arabid

6 T-DNA insertion mutants have been widely used to define

7 T-DNA insertion mutants of CIAF1 lack Complex I and the

8 T-DNA insertions in At3g50740 cause a sugar-insensitive

9 T-DNA insertions in the genes represented by some cDNAs

10 T-DNA insertions in UKL1 and UKL2 reduced transcript exp

11 T-DNA insertions were identified in four of the five Ara

12 T-DNA inserts are stable; no transgene rearrangements we

13 T-DNA knock-out mutants of At3g57630 showed a truncated

14 T-DNA knockout of AtcpFHy/PyrP1 did not affect the flavi

15 T-DNA/plant DNA junctions from these transformed rice an

16 lanking sequence tags (FST) for over 325,000 T-DNA insertion lines.

17 336,000 T-DNA transformed lines were screened for mutants with d

18 id occur in plants homozygous for the dcl3-1 T-DNA insertion and was unaffected by loss of function o

19 KK3, all located within 20 kb of the mekk1-1 T-DNA insertion.

20 o add to these resources we sequenced 21 165 T-DNA lines, 15 569 of which were produced in this study

21 HC-Pro transgenic Arabidopsis and the arf8-6 T-DNA insertion mutant showed little effect on the P1/HC

22 A T-DNA insertion into the Arabidopsis gene (At5g14090) mo

23 A T-DNA insertional atos9-t mutation blocks the degradatio

24 A T-DNA knock-out line did not segregate homozygous mutant

25 A T-DNA mutagenesis screen performed on haploid spores ide

26 A T-DNA mutant eif4a1 line, with reduced eIF4A protein lev

27 A T-DNA-tagged null mutant mtppt-1 allele shows an embryo-

28 localization and the phenotypes of rh3-4, a T-DNA insertion mutant allele of RH3.

29 We next analyzed a T-DNA insertion in the MEKK2 promoter region and found t

30 erance of an ERF74 overexpression line and a T-DNA insertion mutant using flow cytometry, transactiva

31 distachyon, we identified a line carrying a T-DNA insertion in one of the two eukaryotic initiation

32 plant genetic transformation when carrying a T-DNA-containing plasmid.

33 grobacterium binary vector, we constructed a T-DNA that contains a CRISPR/Cas9 system using SpCas9 an

34 rk, Arabidopsis thaliana plants containing a T-DNA disruption of the bile acid sodium symporter BASS6

35 We found that single mutants containing a T-DNA in each of the five Arabidopsis CSLC genes had nor

36 d dark-brown phenotype was identified from a T-DNA-tagged rice mutant library.

37 reactive oxygen species were monitored in a T-DNA insertion mutant of AHK5.

38 Seedling growth was severely reduced in a T-DNA insertion mutant of ICE1, ice1-2, when grown on 1/

39 Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thal

40 RTP1 was identified by screening a T-DNA insertion mutant population and encoded an endopla

41 es underlying root growth variation; using a T-DNA line candidate approach, we identified one gene in

42 AAP2 T-DNA insertion lines showed changes in source-sink tran

43 ovement in response to root flooding in aco5 T-DNA insertion mutants.

44 KEY MESSAGE: This study addresses T-DNA insert stability and transgene expression consiste

45 This study addresses T-DNA insert stability and transgene expression consiste

46 s the major model proposed for Agrobacterium T-DNA integration into the plant genome.

47 proteins required for NHEJ, in Agrobacterium T-DNA integration.

48 combinase was delivered on the Agrobacterium T-DNA injected at the axillary bud site, resulting in th

49 is is typically achieved using Agrobacterium T-DNA, biolistics or by stably integrating nuclease-enco

50 repp12 and ala3 T-DNA mutants show defects in many auxin-regulated proce

51 Allelic T-DNA lines differed for most traits at AT1G11060 but no

52 A biosensor was capable of detecting 0.2 aM. T-DNA.

53 d polQ mutant Arabidopsis and rice, analyzed T-DNA/plant DNA junction sequences, and (for Arabidopsis

54 a putative function of AtVDACs, we analyzed T-DNA insertion lines in each of the corresponding genes

55 Missense (shot1-1) and T-DNA insertion (shot1-2) mutants suppress the hot1-4 he

56 pressing the wild type version of bZIP16 and T-DNA insertion mutants for bZIP68 and GBF1 demonstrated

57 ng term transgene expression consistency and T-DNA insert stability can be achieved in sugarcane, sug

58 ng term transgene expression consistency and T-DNA insert stability can be achieved in sugarcane, sug

59 The discrimination between glu-5-hmC-DNA and T-DNA by DB-JBP1 is about 2-fold less, but enough for DB

60 ith Agrobacterium DNA transfer machinery and T-DNA.

61 eutrons (used for the induction of rfc4) and T-DNA (used for the induction of ife, which is not linke

62 were characterized, along with wild-type and T-DNA knock-down plants.

63 ted T-DNA insertion events, we built another T-DNA with a promoterless hptII gene adjacent to the T-D

64 eport the characterization of an Arabidopsis T-DNA insertion mutant of the MED14 gene.

65 le of functionally complementing Arabidopsis T-DNA insertion mutants in this gene.

66 in the present study we isolated Arabidopsis T-DNA insertion knockout mutant lines for each of the ge

67 We obtained Arabidopsis T-DNA knockout lines for the two genes and analyzed thei

68 of the wild-type level in three Arabidopsis T-DNA insertion mutants (naa20-1, naa20-2, and naa25-1)

69 Two Arabidopsis T-DNA insertion mutant lines with insertions in the prom

70 Two Arabidopsis T-DNA insertion mutants were identified as null mutants

71 We used Arabidopsis T-DNA insertion lines to generate a double mutant in whi

72 Using Arabidopsis T-DNA insertion mutants, we found that their Arabidopsis

73 atmyb2 T-DNA insertion lines have enhanced expression of cytoki

74 sion of AtSLD1 in Arabidopsis plants, AtSLD1 T-DNA mutants showed large reductions in Delta8 unsatura

75 Although all six single AtUXS T-DNA mutants and the uxs1 usx2 uxs4 triple mutant show

76 Through examining all available T-DNA insertional mutants of Arabidopsis MCTPs, we furth

77 tumefaciens requires the import of bacterial T-DNA and virulence proteins into the plant cell that ev

78 Because T-DNA preferentially integrates into double-strand DNA b

79 nstrate that catalytically active Osa blocks T-DNA transfer into plants.

80 Whereas depletion of B14.7 by T-DNA insertion is lethal, Tim23-2 can be depleted witho

81 Disruption of AtPAT14 by T-DNA insertion resulted in an accelerated senescence ph

82 of the DUF579 family have been disrupted by T-DNA insertions contain less xylose in the secondary ce

83 nent), which on simultaneous inactivation by T-DNA insertion lines displayed a severely delayed and c

84 lt plant while knocking down miR408 level by T-DNA insertions or the artificial miRNA technique cause

85 Loss-of-function mutation by T-DNA insertion in AtPAT21 results in the complete failu

86 er to further investigate the route taken by T-DNA molecules on their way to integration.

87 Here, we isolated two mutants that carry T-DNA insertions at the At1g31870 locus and shows early

88 Plants carrying T-DNA insertions in three AtGATL genes (atgatl3, atgatl6

89 duce natural transformants carrying cellular T-DNA (cT-DNA) sequences of bacterial origin.

90 We recovered and characterized T-DNA and transposon-tagged knockout alleles of the MCCA

91 light and plastid signals by characterizing T-DNA insertion alleles of genes that are regulated by l

92 We recovered circular T-DNA molecules (T-circles) using a novel plasmid-rescue

93 Cognate T-DNA knock-down lines display reduced DHNA-CoA thioeste

94 or the formation of extrachromosomal complex T-DNA structures that subsequently may integrate.

95 am) and Musa acuminata (banana) also contain T-DNA-like sequences.

96 gh double knockdown mutant plants containing T-DNA insertions in both genes are embryonic lethal, und

97 Seeds containing T-DNA insertions in CTF7 exhibit mitotic defects in the

98 strated by the analysis of the corresponding T-DNA insertional lines.

99 RFA complex in tobacco resulted in decreased T-DNA expression, as determined by infection with A. tum

100 eased stable transformation due to decreased T-DNA integration.

101 ted T4SS in vitro, we show that Osa degrades T-DNA in the T-DNA-VirD2 complex before its translocatio

102 m, enter the nucleus, and eventually deliver T-DNA to plant chromatin.

103 wth attenuation in plants of a transfer DNA (T-DNA) insertion mutant of WRKY70 (wrky70) suggest that

104 een a collection of individual transfer DNA (T-DNA) insertion mutants.

105 revealed that double-stranded transfer DNA (T-DNA) intermediates can serve as substrates by as yet u

106 ion transgenic lines or in the transfer-DNA (T-DNA) insertion mutant lbd29-1, enhanced SCW developmen

107 The integration of transferred DNA (T-DNA) and the formation of complex insertions in the ge

108 arious plants by delivering transferred DNA (T-DNA) and virulence proteins into host plant cells.

109 f Agrobacterium tumefaciens transferred DNA (T-DNA) into the plant genome is the last step required f

110 nts by transferring a region of plasmid DNA, T-DNA, into host plant cells.

111 an oncogenic piece of DNA (transferred DNA, T-DNA) into plant cells at the infection site.

112 In the presence of target DNAs (T-DNA), hairpin probes hybridized with T-DNAs to form a

113 Although single or double T-DNA mutant tcp8, tcp14 or tcp15 lines were not more su

114 evidence suggests that double-stranded (ds) T-DNA, converted from T-strands, are potent substrates f

115 hat of wild-type plants, suggesting enhanced T-DNA integration in these mutants.

116 Etiolated T-DNA insertion mutants were screened for red fluorescen

117 Here, we show that complex extrachromosomal T-DNA structures form in A. tumefaciens-infected plants

118 t triple mutant c1,c3,fkf1-t carrying a FKF1 T-DNA insertion mutant.

119 200 times higher than the JBP1 affinity for T-DNA.

120 na NHEJ genes, and by biochemical assays for T-DNA integration.

121 hromosome, providing greater opportunity for T-DNA to integrate.

122 In this study, we searched for T-DNA-like genes in the sequenced genomes of dicots and

123 s for two additional rice varieties and four T-DNA tagged transformants from the Taiwan Rice Insertio

124 Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles o

125 ss the functional significance of the genes, T-DNA mutants were identified.

126 N. benthamiana and Arabidopsis thaliana GOX T-DNA insertion mutants are compromised for nonhost resi

127 Homozygous T-DNA insertion lines were thus obtained for five of the

128 We phenotyped multiple homozygous T-DNA A. thaliana mutants at each of two loci (AT1G11060

129 Here we identified T-DNA insertion mutants in three alpha subunit genes (al

130 ther multiple redundant pathways function in T-DNA integration, and/or that integration requires some

131 a (PolQ) may be a crucial enzyme involved in T-DNA integration.

132 er we examine callose deposition patterns in T-DNA insertion mutants (cs7) of the Callose Synthase 7

133 eased stable transformation due to increased T-DNA integration.

134 Three independent T-DNA insertional mutant lines deficient in 6-phosphofru

135 ons of Arabidopsis thaliana sequence-indexed T-DNA insertion mutants are among the most important res

136 so developed inexpensive methods for INTACT, T-DNA insertion mapping, and profiling of the complete n

137 Integrated T-DNA molecules typically exhibit deletions at their 3'

138 abidopsis) measured the amount of integrated T-DNA in mutant and wild-type tissue.

139 iguration and genomic position of integrated T-DNA molecules likely affect transgene expression, and

140 on of the donor template from pre-integrated T-DNA.

141 T-DNA mutants we observed were all intronic T-DNA mutants and the T-DNA fragments in both the trigge

142 demonstrate that the suppression of intronic T-DNA mutants is mediated by trans-interactions between

143 k shows that caution is needed when intronic T-DNA mutants are used.

144 itates proteasomal uncoating of the invading T-DNA from its associated proteins.

145 ucosinolate profile of an Arabidopsis ipmdh1 T-DNA knock-out mutant could be restored to wild-type le

146 f the host cell, Agrobacterium transfers its T-DNA--as a complex (T-complex) with the bacterial VirE2

147 tterns include illegitimate DNA end joining, T-DNA truncations, T-DNA repeats, binary vector sequence

148 ts failed to rescue the respective knockout (T-DNA insertion) mutants, indicating that pigment-bindin

149 Analysis of LDIP T-DNA knockdown and knockout mutants showed a decrease i

150 junctions carry a single copy of full-length T-DNA at the target site.

151 Arf8-6 is a SALK line T-DNA insertion mutant, a class of mutations prone to in

152 using either artificial microRNAs or a LOG2 T-DNA insertion.

153 ation of a new tomato (Solanum lycopersicum) T-DNA mutant allowed for the isolation of the CALCINEURI

154 Arabidopsis reference genome sequence to map T-DNA flanking sequence tags (FST) for over 325,000 T-DN

155 Agrobacterium-mediated T-DNA integration into the plant genomes is random, whic

156 The MEF8 T-DNA insertion (mef8) line exhibited reduced editing at

157 a CMP or Ubi promoter, were used to monitor T-DNA insert stability and consistency of transgene enco

158 d Arabidopsis GTG1 and GTG2 and isolated new T-DNA insertion alleles of GTG1 and GTG2 in both Wassile

159 In the nhx5 nhx6 T-DNA insertional knockouts, the precursors of the 2S al

160 Analyses of T-DNA insertion mutants for each of these candidate PSS1

161 Analyses of T-DNA insertion victr alleles showed that VICTR is neces

162 tivity does not alter the characteristics of T-DNA integration events.

163 Characterization of T-DNA insertion mutant alleles at each AACT locus establ

164 ene network modeling and characterization of T-DNA mutants indicated that acyl-activating enzyme enco

165 s are present in a widely-used collection of T-DNA insertion lines, we analyzed 64 independent lines

166 MMA was proportional to the concentration of T-DNA and quantified by square wave voltammetry.

167 ation event, permitting stable expression of T-DNA-encoded transgenes.

168 ium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir) effector proteins

169 Integration of T-DNA into the plant genome establishes a permanent tran

170 T-DNA right border such that integration of T-DNA into the targeted exon sequence in-frame with the

171 somal replicons or off-target integration of T-DNA or replicon sequences.

172 t facilitate the transfer and integration of T-DNA.

173 ts suggest that a cautious interpretation of T-DNA phenotypes is warranted.

174 Here we show that mature leaves of T-DNA insertion lines with diminished expression of PRX3

175 inhibited the transient expression level of T-DNA and only reduced T-DNA integration by 50% suggests

176 ver, mutation of parp1 caused high levels of T-DNA integration and transgene methylation.

177 The mechanism of T-DNA integration remains controversial, although scient

178 al identified target genes by phenotyping of T-DNA mutants.

179 Plants of T-DNA insertion mutants, lacking FUM2, show marked diffe

180 may result from asymmetric polymerization of T-DNA's ends.

181 iews our current knowledge of the process of T-DNA integration and proposes ways in which this knowle

182 signaling, we examined ABA sensitivities of T-DNA mutants of a number of Arabidopsis thaliana DWD ge

183 cted phenomenon of epigenetic suppression of T-DNA insertion mutants in Arabidopsis.

184 We show that epigenetic suppression of T-DNA mutants is not a rare event, but certain structura

185 m attachment to plant cells and transport of T-DNA to the nucleus have been identified, but the T-DNA

186 Several DNA substrates (oncogenic T-DNA and plasmids RSF1010 and pCloDF13) induced the Vir

187 We observed one T-DNA line with normal pollen that nevertheless had a tr

188 how any decrease in stable transformation or T-DNA integration.

189 Analysis of single and higher-order T-DNA insertion jaz null mutants provided further eviden

190 ree triple mutants harboring non-overlapping T-DNA insertions in cytokinin AHK receptors.

191 ted by complementing homozygous lethal OXA2b T-DNA insertional mutants with a C-terminally truncated

192 of DNAPs, even for perfectly complementary P/T DNA sequences.

193 nd near the coding base of the template in P/T DNA complexes with Klenow fragment (KF) DNAP as the po

194 conformational changes in primer-template (P/T) DNA are involved in the selective incorporation of dN

195 of a portion of its tumor-inducing plasmid (T-DNA) into the plant genome.

196 Plants homozygous for pskr1 T-DNA insertions showed enhanced defense gene expression

197 Finally, pyl6 T-DNA mutant plants show an increased sensitivity to the

198 For a number of QTLs, T-DNA insertion knockout lines could validate assigned c

199 as identified in a Beauveria bassiana random T-DNA insertion library.

200 t expression level of T-DNA and only reduced T-DNA integration by 50% suggests that double-stranded T

201 ter-trap transformation vector that requires T-DNA integration into the plant genome to activate a pr

202 fer to immature embryos using two respective T-DNAs.

203 Here over 20,000 M. robertsii T-DNA mutants were screened in order to elucidate geneti

204 Analysis of rpa1 T-DNA insertion mutants demonstrates that although each

205 alt stress-related genes with available SALK T-DNA mutagenesis lines for phenotypic screening and ide

206 el mutant screen, combining a confirmed SALK T-DNA insertion collection with traditional forward gene

207 of more than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonge

208 analyzed 64 independent lines from the Salk T-DNA mutant collection.

209 ilencing induced by the dcl3-1 (SALK_005512) T-DNA insertion mutant line.

210 own promoter and terminator, within the same T-DNA.

211 e used a reverse genetics approach to screen T-DNA insertion mutants corresponding to all 47 of the A

212 We therefore screened T-DNA insertion mutants in these RLKs for root hair defe

213 imental procedures for efficiently screening T-DNA lines for the presence of chromosomal abnormalitie

214 ophysical and structural studies of the 2-Se-T DNAs reveal that the bulky 2-Se atom with a weak hydro

215 lyses showed that four out of five sequenced T-DNA/gDNA junctions carry a single copy of full-length

216 Single T-DNA mutants and double artificial microRNA knockdowns

217 -optimized Escherichia coli BirA in a single T-DNA construct.

218 Southern blot analyses indicated a single T-DNA insertion in the mutant, located on chromosome 10.

219 ith impaired pollen development and a single T-DNA insertion in the transcription factor gene bHLH142

220 pproximately 40% for Arabidopsis msr1 single T-DNA insertion mutants and by more than 50% for msr1 ms

221 lection, we phenotypically characterized six T-DNA lines with insertions in genes previously shown in

222 homology-dependent silencing induced by some T-DNA insertion mutant lines is siRNA-mediated.

223 Thus, some T-DNA insertion mutant lines induce 35S promoter homolog

224 Accordingly, homozygous ssSPTa T-DNA mutants were not recoverable, and 50% nonviable po

225 gration by 50% suggests that double-stranded T-DNA intermediates, as well as single-stranded T-DNA, p

226 of the T-complex include the single stranded T-DNA, bacterial virulence proteins (VirD2, VirE2, VirE3

227 A integration, a transferred single-stranded T-DNA is converted into a double-stranded form.

228 NA intermediates, as well as single-stranded T-DNA, play significant roles in the integration process

229 opose that termini of linear double-stranded T-DNAs are recognized and repaired by the plant's DNA do

230 A strong T-DNA insertion allele, ftsHi1-2, caused embryo-lethalit

231 The suppressed T-DNA mutants we observed were all intronic T-DNA mutant

232 e trigger T-DNA as well as in the suppressed T-DNA shared stretches of identical sequences.

233 In an effort to detect targeted T-DNA insertion events, we built another T-DNA with a pr

234 as9 system can efficiently generate targeted T-DNA insertions.

235 transformation can lead to precise targeted T-DNA integration in the rice genome.

236 that these constructs could produce targeted T-DNA insertions with frequencies ranging between 4 and

237 SPR/Cas9 system to demonstrate that targeted T-DNA integration can be achieved in the rice genome.

238 Previous reports have indicated that T-DNA insertion lines can have chromosomal translocation

239 Various studies suggest that T-DNA is protected inside host cells by VirE2, one of th

240 The T-DNA also carried a red fluorescent protein and a hygro

241 The T-DNA insertion mutant of MSRB8 exaggerates HR-associate

242 The T-DNA knockout mutant hcf222-2 showed a more severe defe

243 rved were all intronic T-DNA mutants and the T-DNA fragments in both the trigger T-DNA as well as in

244 The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-let

245 to the nucleus have been identified, but the T-DNA integration step during transformation is poorly u

246 The lesion caused by the T-DNA insertion localizes to the promoter region, result

247 racterization of a mutant that contained the T-DNA insertion in the promoter region of the TK1a gene.

248 in/F-box protein VBF pathway and exposes the T-DNA molecule to external enzymatic activity.

249 Nn F1 offspring selected for the T-DNA develop normally under sterile conditions.

250 the number of unique insertion sites in the T-DNA collection by 21 078, bringing the overall total t

251 ter-directed cytokinin oxidase 1 gene in the T-DNA insertion lines reduces the endogenous cytokinin l

252 ve during early stages of development in the T-DNA insertional mutant.

253 vent, but certain structural features in the T-DNA mutants are needed in order for the suppression to

254 itro, we show that Osa degrades T-DNA in the T-DNA-VirD2 complex before its translocation.

255 , we could select double mutants lacking the T-DNA already in the first offspring generation.

256 In the mutant, the T-DNA insertion caused a 40 % rise in transcript levels

257 ransmitted to T2 plants independently of the T-DNA construct.

258 ed opr3, potentially through splicing of the T-DNA containing intron.

259 Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided i

260 immediately flanking the right border of the T-DNA insertion, which encoded an uncharacterized Broad

261 e present an overview of the location of the T-DNA inserts of all lines, with one exception.

262 e presence of a translocation, all 11 of the T-DNA lines showing an abnormal pollen phenotype were fo

263 able to complement the growth defects of the T-DNA lines.

264 This mutant contained a copy of the T-DNA tag inserted at the location where the expression

265 Two assays were used to screen the T-DNA lines for translocations: pollen viability and gen

266 DNA gel blot analysis showed that the T-DNA inserts are stable; no transgene rearrangements we

267 our genetic mapping experiments was that the T-DNA junctions on the 5'- and 3'-sides of a targeted ge

268 There the T-DNA is uncoated from its protein components before und

269 induction of ife, which is not linked to the T-DNA present in the line) can result in the duplication

270 th a promoterless hptII gene adjacent to the T-DNA right border such that integration of T-DNA into t

271 indicating that this activity can unmask the T-DNA molecule.

272 ght, heat and aluminum stresses, whereas the T-DNA insertion mutant erf74 and the erf74;erf75 double

273 romosomal translocations associated with the T-DNA insertion site, but the prevalence of these rearra

274 orted into host cells concomitantly with the T-DNA to effectuate transformation.

275 results revealed that exon 5, along with the T-DNA, is removed in this mutant, resulting in a truncat

276 o of the lines in our survey displayed this 'T-DNA borders separate' phenomenon.

277 Here, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrom

278 Thus, T-DNA integration does not require known NHEJ proteins,

279 Our data suggest that prior to T-DNA integration, a transferred single-stranded T-DNA i

280 Like pskr1 mutants, a tpst T-DNA insertion line exhibited enhanced MAMP-triggered s

281 Furthermore, a transfer (T)-DNA insertional alg3-T2 mutation, which causes accumu

282 Repeated selfing of pgd2 transfer (T-)DNA alleles yielded no homozygous mutants, although s

283 airy roots, due to expression of transferred T-DNA genes.

284 and the T-DNA fragments in both the trigger T-DNA as well as in the suppressed T-DNA shared stretche

285 gitimate DNA end joining, T-DNA truncations, T-DNA repeats, binary vector sequences, and other unknow

286 over conventional Agrobacterium tumefaciens T-DNA.

287 s mediated by trans-interactions between two T-DNA insertions.

288 l the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, center

289 When the two T-DNA insertion mutants, yuc1-1 and ag-TD, were crossed

290 Compared to the segregation of uncoupled T-DNAs in conventionally produced progeny, the incorpora

291 Using T-DNA insertion lines, we isolated a novel deletion muta

292 Functional studies using T-DNA insertion mutants reveal that they can act as anti

293 Disruption of AtNDB2 expression via T-DNA insertion led to a 90% decrease of external NADH o

294 CML38-knockout mutants generated via T-DNA insertion were insensitive to AtRALF1, and simulta

295 the past decade, phenotypes identified with T-DNA-induced mutants have played a critical role in adv

296 acid (ABA) but is increased in mutants with T-DNA insertions in the FLDH 5' flanking region.

297 Plants with T-DNA disruptions in RBL10 have greatly decreased 16:3 (

298 DNAs (T-DNA), hairpin probes hybridized with T-DNAs to form a duplex DNA, and the ring of hairpin DNA

299 Compared with the wild type, WRKY22 T-DNA insertion mutants wrky22-1 and wrky22-2 had lower

300 Furthermore, the zml1 and zml2 T-DNA insertion lines displayed a high irradiance-sensit