ライフサイエンスコーパス: TBSV

1 TBSV hijacks Rab1 and COPII vesicles to create enlarged

2 TBSV VRCs assembled on GUVs provide significant protecti

3 TBSV-driven usurping of Rab7 has proviral functions thro

4 on in yeast, which supports replication of a TBSV replicon RNA (repRNA), reduced repRNA accumulation

5 he remainder decreased the accumulation of a TBSV replicon RNA.

6 increased or decreased the accumulation of a TBSV replicon RNA.

7 d RdRp could perform de novo initiation on a TBSV plus-strand RNA template in the presence of the p33

8 Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detecta

9 ents with ssRNA revealed that p33 binds to a TBSV-derived sequence with higher affinity than to other

10 elective p33 binding in vitro also abolished TBSV RNA replication both in plant and in Saccharomyces

11 re required for reconstitution of the active TBSV VRCs in GUVs, further supporting that the novel GUV

12 In addition, TBSV-NPs do not show capsomeric vacancies after surpassi

13 ial and nonessential host genes could affect TBSV recombination and evolution.

14 ella effectors tested, 28 effectors affected TBSV replication.

15 SARS-CoV-2, and two HMPV proteins affecting TBSV recombination likely target shared host factors wit

16 O2, is involved in antiviral defense against TBSV.

17 rping the GTP-Rab5-positive endosomes allows TBSV to build a PE-enriched viral replication compartmen

18 This strategy likely allows TBSV to protect the replicating viral RNA from degradati

19 Altogether, this replication strategy allows TBSV to separate minus- and plus-strand syntheses in tim

20 tibility and restriction factors for BMV and TBSV have been identified using yeast as a model host.

21 The dependence of TMV, PVX, and TBSV on intact microfilaments for intercellular movement

22 the identified bacterial effectors with anti-TBSV activity could be powerful reagents in cell biology

23 is shown to be directly associated with anti-TBSV RNA silencing, while its inactivation does not infl

24 The assembled TBSV replicase performed a complete replication cycle, s

25 vide evidence that the peroxisome-associated TBSV and the mitochondria-associated carnation Italian r

26 strongly inhibits the peroxisome-associated TBSV and the mitochondria-associated CIRV replication in

27 was shown in a cell-free yeast extract-based TBSV replication assay, in which Pkc1p likely phosphoryl

28 ggests that there is a race going on between TBSV and its host to exploit the actin network and ultim

29 s within the CRAC and CARC sequences blocked TBSV replication in yeast and plant cells.

30 expression of DrrA in yeast or plants blocks TBSV replication through inhibiting the recruitment of R

31 n-dependent protein catabolism affected both TBSV replication and the cytotoxicity of a mutant huntin

32 with vir-condensate substructures driven by TBSV p33 replication-associated protein.

33 ction of the endoribonucleolytically cleaved TBSV RNA in yeast.

34 ing that the 3' portion of the miRNA-cleaved TBSV RNAs served as a template for negative-strand RNA s

35 o led to decreased production of the cleaved TBSV RNA, suggesting that in plants, RNase MRP is involv

36 tive mutants of plant Rab5 greatly decreases TBSV replication and prevents the redistribution of PE t

37 a cellular proviral dependency factor during TBSV replication.

38 fects the recruitment of host factors during TBSV replication.

39 lin have similar inhibitory functions during TBSV replication, although some of the details of their

40 As were amplified to very high levels during TBSV infection.

41 PI(4)P play important proviral roles during TBSV replication.IMPORTANCE Replication of positive-stra

42 SARS-CoV-2 N and HMPV M2-1 proteins enhance TBSV RNA replication and recombination by protecting the

43 neumovirus M2-1 protein are shown to enhance TBSV RNA replication and recombination by protecting the

44 al replication proteins that is critical for TBSV replication.IMPORTANCE One intriguing aspect of vir

45 irming that CypA is a restriction factor for TBSV.

46 in Saccharomyces cerevisiae, is required for TBSV replication in the yeast model host.

47 t cytosolic chaperone, which is required for TBSV replication.

48 ol, suggesting that sterols are required for TBSV replication.

49 stranded RNA that serves as the template for TBSV replication.

50 TPR-containing yeast proteins in a cell-free TBSV replication assay and identified the Cns1p cochaper

51 well described by the A subunit pentons from TBSV.

52 wever, the source of energy required to fuel TBSV replication is unknown.

53 l-free system was also capable of generating TBSV RNA recombinants with high efficiency.

54 ions in viral shell stability and identifies TBSV-NPs as malleable platforms based on protein cages f

55 applying a chloride channel blocker impeded TBSV replication in Nicotiana benthamiana protoplasts or

56 tin-conjugating enzyme function of Cdc34p in TBSV replication.

57 itide and the early endosomal compartment in TBSV replication.

58 nderstanding of the roles of host factors in TBSV replication, we have tested the effect of Rsp5p, wh

59 e functions of viral and cellular factors in TBSV replication.IMPORTANCE Understanding the mechanism

60 er our understanding on the role of GAPDH in TBSV replication, we used an in vitro TBSV replication a

61 To dissect the function of Hsp70 in TBSV replication, in this paper we use an Hsp70 mutant (

62 ing that in plants, RNase MRP is involved in TBSV RNA degradation.

63 e large family of host prolyl isomerases, in TBSV replication.

64 es the novel functions of Sac1 and PI(4)P in TBSV replication in the model host yeast and in plants.

65 To unravel a coopted cellular pathway in TBSV replication, the identified DrrA effector from Legi

66 tidylserine and phosphatidylethanolamine, in TBSV replication.

67 f the critical role of phosphatidylserine in TBSV replication and a novel role for phosphatidylethano

68 ning the relevance of these host proteins in TBSV replication.

69 e; APB) leads to a 3- to 5-fold reduction in TBSV replication in yeast.

70 cleaves the TBSV RNA in vitro, resulting in TBSV RNA degradation products similar in size to those o

71 at the co-opted GAPDH plays a direct role in TBSV replication by stimulating plus-strand synthesis by

72 otein, play partially complementary roles in TBSV replication in cells and in cell extracts.

73 that cytosolic Hsp70 plays multiple roles in TBSV replication, such as affecting the subcellular loca

74 We demonstrate a critical role for Sac1 in TBSV replicase assembly in a cell-free replicase reconst

75 our understanding of the role of sterols in TBSV replication, we demonstrate that the downregulation

76 e downregulation of Rsp5p leads to increased TBSV accumulation.

77 itor of Pkc-like kinases, leads to increased TBSV replication in yeast, in plant single cells, and in

78 sive temperature, TS Cns1p could not inhibit TBSV replication.

79 tive mutant of CypA was also able to inhibit TBSV replication in vitro due to binding to the replicat

80 ll, blocking Gef1p function seems to inhibit TBSV replication through altering Cu(2+) ion metabolism

81 Overexpression of Cns1p inhibited TBSV replication in yeast.

82 cleotide exchange factors) of Rab7 inhibited TBSV RNA replication in yeast.

83 nant CypA, Roc1, and Roc2 strongly inhibited TBSV replication in a cell-free replication assay.

84 -binding protein in yeast strongly inhibited TBSV replication.

85 in many cellular processes, which inhibited TBSV replication when overexpressed.

86 terestingly, recombinant Rsp5p also inhibits TBSV RNA replication in a cell-free replication assay, l

87 e find that overexpression of Rsp5p inhibits TBSV replication in Saccharomyces cerevisiae yeast, whil

88 e and retromer biogenesis, strongly inhibits TBSV and CIRV replication in yeast and in planta.

89 Moreover, APB treatment inhibits TBSV RNA accumulation in plant protoplasts and in Nicoti

90 -free system also replicated the full-length TBSV genomic RNA, which resulted in production of subgen

91 e host factors, while unlike the full-length TBSV RdRp, the truncated RdRp did not need the viral p33

92 gether, our data reveal that Gef1p modulates TBSV replication via regulating Cu(2+) metabolism in the

93 dual tomato bushy stunt virus nanoparticles (TBSV-NPs).

94 mechanical deformations performed on native TBSV-NPs induce an analogous result.

95 ospholipids, sterols, and the actin network, TBSV exerts supremacy over the host cell to support vira

96 plementary RNA as template to synthesize new TBSV replicon RNA.

97 io of TBSV recombinants to the nonrecombined TBSV RNA.

98 y associated with duplex approximately 21-nt TBSV siRNAs, while P19/75-78 does not bind these molecul

99 n complex that contained approximately 21-nt TBSV-derived siRNAs and that exhibited ribonuclease acti

100 al preparations, suggesting that assembly of TBSV and CIRV replicases could take place in the purifie

101 ion of the lethal syndrome characteristic of TBSV infections.

102 cannot prevent RISC-mediated degradation of TBSV RNA and thus reduce viral pathogenicity.

103 s for ribonuclease activity and detection of TBSV-derived siRNAs.

104 y of p92(pol), with consequent inhibition of TBSV replicase activity.

105 teractions, is responsible for inhibition of TBSV replication, whereas the HECT domain, involved in p

106 east Cpr1p cyclophilin, a known inhibitor of TBSV replication in yeast.

107 nd Hsp90 chaperones as a strong inhibitor of TBSV replication.

108 rting the idea that Pkc1p is an inhibitor of TBSV RNA replication.

109 kc-related pathways are potent inhibitors of TBSV in several hosts.

110 ive mutant of Pkc1p revealed a high level of TBSV replication at a semipermissive temperature, furthe

111 2) yeast strain that supports a low level of TBSV replication.

112 uplicated AU-rich sequences, the majority of TBSV DI RNA recombinants were imprecise.

113 ase experiments showed that the mechanism of TBSV replication involves the use of dsRNA templates in

114 gionella effectors reduced the production of TBSV recombinants in yeast and plants.

115 VROs, which showed diminished protection of TBSV p33 and the viral RNA from degradation and also red

116 t either increased or decreased the ratio of TBSV recombinants to the nonrecombined TBSV RNA.

117 wide screens reveals that the replication of TBSV and brome mosaic virus (BMV), which belongs to a di

118 nce of cytosolic Hsp70 in the replication of TBSV and other plant viruses in a plant host.

119 transferase in yeast enhances replication of TBSV and other viruses, suggesting that abundant PE in s

120 capsid is essentially identical with that of TBSV, and the T=1 particles are well described by the A

121 The inhibitory effect of APB on TBSV replication can be complemented by exogenous stigma

122 ect inhibitory activity of LegC8 effector on TBSV replication using a cell-free replicase reconstitut

123 sitol-3-phosphate [PI(3)P] and ergosterol on TBSV replication.

124 ry effect of deletion of CCC2 copper pump on TBSV replication in yeast, while altered iron metabolism

125 ranscribed in vitro were mixed with parental TBSV transcripts and inoculated into protoplasts or plan

126 , uses a similar strategy to the peroxisomal TBSV to hijack the Rab5-positive endosomes into the vira

127 ber of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-in

128 Using purified recombinant TBSV and CIRV replication proteins, we showed that TBSV

129 replicase required two purified recombinant TBSV replication proteins, which were obtained from E. c

130 ilencing complex cleavage of the recombinant TBSV RNAs.

131 ion of an N-terminally truncated recombinant TBSV RdRp.

132 while altered iron metabolism did not reduce TBSV replication.

133 rthologs of ERG25, in N. benthamiana reduced TBSV RNA accumulation but had a lesser inhibitory effect

134 ol biosynthesis inhibitor lovastatin reduced TBSV replication by 4-fold, confirming the importance of

135 t observed in rpn11 mutant yeast by reducing TBSV recombination.

136 levels in yeast and plant cells replicating TBSV.

137 ained from yeast and plant cells replicating TBSV.

138 surrogate host and plant leaves replicating TBSV.

139 T-I or ESCRT-III deletion yeasts replicating TBSV RNA, demonstrating the requirement for these co-opt

140 med in ESCRT-III deletion yeasts replicating TBSV RNA.

141 ete replication cycle on added plus-stranded TBSV replicon RNA (repRNA) that led to the production of

142 ties of p33 to bind to sterol and to support TBSV replication in yeast and plant cells.

143 in Saccharomyces cerevisiae, which supports TBSV replication.

144 in this paper, the authors demonstrate that TBSV co-opts the guanosine triphosphate (GTP)-bound acti

145 Here, we demonstrate that TBSV p33 and p92 replication proteins can bind to sterol

146 We demonstrate that TBSV p33-driven retargeting of Rab7 into VROs results in

147 We demonstrate that TBSV p33-driven retargeting of the retromer into VROs re

148 Altogether, we demonstrated that TBSV is less limited while CIRV is more restricted in ut

149 We find that TBSV co-opts the cellular glycolytic ATP-generating pyru

150 We show that TBSV p33 and the CIRV p36 replication proteins sequester

151 nd CIRV replication proteins, we showed that TBSV could use the purified yeast ER and mitochondrial p

152 pids are the most efficient, suggesting that TBSV replicates within membrane microdomains enriched fo

153 Interaction between Rab7 and the TBSV p33 replication protein leads to the recruitment of

154 ate for negative-strand RNA synthesis by the TBSV RNA-dependent RNA polymerase (RdRp), followed by te

155 highly purified yeast RNase MRP cleaves the TBSV RNA in vitro, resulting in TBSV RNA degradation pro

156 The formation of the TBSV replicase required two purified recombinant TBSV re

157 GUV-based reconstitution of the TBSV replicase revealed the need for a complex mixture o

158 es essential coopted cellular factors of the TBSV replication process.

159 approach recapitulates critical steps of the TBSV replication process.

160 d transport both affected replication of the TBSV replicon and enhanced the cytotoxicity of the Parki

161 to the in vivo situation, replication of the TBSV replicon RNA took place in a membraneous fraction,

162 reduced or increased the accumulation of the TBSV replicon.

163 atitis C virus to specifically recognize the TBSV IRE.

164 In addition to faithfully replicating the TBSV replicon RNA, the cell-free system was also capable

165 The experiments demonstrated that the TBSV (-)RNA is present as a double-stranded RNA that ser

166 replication, in this work we showed that the TBSV p33 and p92 replication proteins could bind to ster

167 The data support the notion that the TBSV replication proteins are associated with sterol-ric

168 Thus, the TBSV/yeast system can be used as a cellular system senso

169 We found that this RNA sequence bound to the TBSV replicase proteins more efficiently than did contro

170 be direct, based on its interaction with the TBSV p33 replication protein, its copurification with th

171 el the mechanism of PE enrichment within the TBSV replication compartment, in this paper, the authors

172 nduced membrane contact sites and within the TBSV replication compartment.

173 yntaxin18-like Ufe1 SNARE protein within the TBSV replication compartments.

174 Then, TBSV utilizes the stable actin filaments as "trafficking

175 studies with tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed the inhibitory

176 us work with Tomato bushy stunt tombusvirus (TBSV) in model host yeast has revealed essential roles f

177 Tomato bushy stunt tombusvirus (TBSV) is a model virus that can replicate a small replic

178 d to inhibit Tomato bushy stunt tombusvirus (TBSV) replication in a Saccharomyces cerevisiae model ba

179 s works with Tomato bushy stunt tombusvirus (TBSV) revealed the recruitment of either peroxisomal or

180 Wild-type TBSV or p19-defective mutants initially show a similar i

181 However, unlike TBSV, there appears to be a novel zinc binding site with

182 Using TBSV, we uncover a race between the virus and its host w

183 Using TBSV, we uncovered the critical roles of Sac1 PI(4)P pho

184 By using TBSV RdRp, we show that the co-opted cellular Hsp70 chap

185 Altogether, the novel strategy of using TBSV as a cellular system sensor might assist in the ide

186 plicase complex of Tomato bushy stunt virus (TBSV) and affects asymmetric viral RNA synthesis.

187 study, we employed tomato bushy stunt virus (TBSV) and carnation Italian ringspot virus (CIRV) - Nico

188 ering (DI) RNAs of tomato bushy stunt virus (TBSV) and have investigated their potential to protect t

189 Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19)

190 e demonstrate that tomato bushy stunt virus (TBSV) and the closely related carnation Italian ringspot

191 e demonstrate that tomato bushy stunt virus (TBSV) and the closely related carnation Italian ringspot

192 The VRCs built by Tomato bushy stunt virus (TBSV) are enriched with phosphatidylethanolamine (PE) th

193 Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mo

194 Tomato bushy stunt virus (TBSV) co-opts cellular ESCRT (endosomal sorting complexe

195 rall, the works on Tomato bushy stunt virus (TBSV) have revealed intriguing and complex functions of

196 in degradation of Tomato bushy stunt virus (TBSV) in a Saccharomyces cerevisiae model host, we teste

197 the replication of Tomato bushy stunt virus (TBSV) in a yeast model host.

198 RNA replication of Tomato bushy stunt virus (TBSV) in yeast cell-free extracts and in plant extracts.

199 er (+)RNA viruses, tomato bushy stunt virus (TBSV) induces major changes in infected cells.

200 busvirus, of which tomato bushy stunt virus (TBSV) is the type member.

201 hat replication of Tomato bushy stunt virus (TBSV) leads to the formation of double-stranded RNA (dsR

202 in replication of Tomato bushy stunt virus (TBSV) model (+)RNA virus.

203 he closely related Tomato bushy stunt virus (TBSV) or Cucumber necrosis virus (CNV) in a yeast model

204 tiana benthamiana, Tomato bushy stunt virus (TBSV) P19 suppressor mutants are very susceptible to RNA

205 activation of the Tomato bushy stunt virus (TBSV) RdRp requires a soluble host factor(s).

206 have reconstituted Tomato bushy stunt virus (TBSV) replicase using artificial giant unilamellar vesic

207 (PE) vesicle-based Tomato bushy stunt virus (TBSV) replication assay.

208 hosphatase reduced tomato bushy stunt virus (TBSV) replication in yeast (Saccharomyces cerevisiae) an

209 tors that modulate tomato bushy stunt virus (TBSV) replication in yeast surrogate host.

210 s interacting with Tomato bushy stunt virus (TBSV) replication proteins in a genome-wide scale, we ha

211 us host factors in Tomato bushy stunt virus (TBSV) replication, we have developed an artificial giant

212 t their effects on tomato bushy stunt virus (TBSV) RNA recombination.

213 t their effects on tomato bushy stunt virus (TBSV) RNA recombination.

214 Previous works on tomato bushy stunt virus (TBSV) showed that the p33 replication protein subverts t

215 ously we described Tomato bushy stunt virus (TBSV) vectors, which retained their capsid protein gene

216 Tomato bushy stunt virus (TBSV), a model (+)RNA virus, assembles membranous VROs,

217 the replication of tomato bushy stunt virus (TBSV), a positive-strand RNA virus of plants.

218 ing replication of Tomato bushy stunt virus (TBSV), a small model plant virus, we screened 800 yeast

219 A recombination in Tomato bushy stunt virus (TBSV), a small model plant virus.

220 ing replication of Tomato bushy stunt virus (TBSV), a small model positive-stranded RNA virus, we ove

221 the replication of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed

222 ion of the RdRp of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we used N-terminal

223 Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded

224 te associated with Tomato bushy stunt virus (TBSV), a tombusvirus, undergoes frequent recombination i

225 the p19 protein of tomato bushy stunt virus (TBSV), that prevents the onset of PTGS in the infiltrate

226 pical tombusvirus, Tomato bushy stunt virus (TBSV), we show that recombinant p33 replicase protein bi

227 cation proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus.

228 similar to that of Tomato bushy stunt virus (TBSV), with major differences lying on the exposed loops

229 The Tomato bushy stunt virus (TBSV)-encoded p19 protein (P19) is widely used as a robu

230 plicase complex of Tomato bushy stunt virus (TBSV).

231 ic virus (BMV) and tomato bushy stunt virus (TBSV).

232 ato virus X (PVX), tomato bushy stunt virus (TBSV)], is inhibited by disruption of microfilaments.

233 Using in vitro TBSV replicase assembly on giant unilamellar vesicles co

234 PDH in TBSV replication, we used an in vitro TBSV replication assay based on recombinant p33 and p92(

235 of GUVs have pronounced effects on in vitro TBSV replication, including (-) and (+)RNA synthesis.

236 hat exhibited ribonuclease activity that was TBSV sequence-preferential, ssRNA-specific, divalent cat

237 ral proteins target shared host factors with TBSV, including the autophagy pathway.

238 ation likely target shared host factors with TBSV.

239 ranous VROs through direct interactions with TBSV p33.

240 By two weeks postinoculation with TBSV, all untransformed N. benthamiana plants and transf

241 Also, as was seen with TBSV, CNV appears to have a calcium binding site between