ライフサイエンスコーパス: TCR-alpha

1 TCR alpha (TCRA) expression was examined in RNA samples

2 TCR alpha beta transgenic (Tg) mice have no such delay,

3 TCR alpha beta+ intestinal intraepithelial lymphocytes (

4 TCR-alpha was also found to be degraded in a proteasome-

5 TCR-alpha-/- x Ig mu-/- mice spontaneously developed col

6 TCR-alpha-deficient mice maintained germfree or colonize

7 The L51S mutation in the D10.G4.1 TCR alpha-chain reduces the affinity of the TCR to its l

8 The Y-Ae mAb and the 1H3.1 TCR-alpha beta (V alpha 1/V beta 6) are two immune recep

9 precise homologues of the subset of NK1.1(+) TCR-alpha/beta+ T cells, often referred to as NK T cells

10 ereas the unusual subset of CD8alpha/alpha(1)TCR-alpha/beta(1) resident IELs recognize nonclassical M

11 or D molecules, whereas the CD8alpha/alpha(1)TCR-alpha/beta(1) subset is independent of classical MHC

12 se findings demonstrate that CD8alpha/beta(1)TCR-alpha/beta(1) IELs are restricted by H-2K and H-2D m

13 nd H2-D(b), we show that the CD8alpha/beta(1)TCR-alpha/beta(1) subset is dependent on K or D molecule

14 ed numbers of invariant V alpha 14J alpha 18 TCR alpha-chain-positive natural T (iNKT) cells that do

15 rs of stimulation with 200 umol/L NiSO(4) ., TCR alpha- and beta-chains of sorted nickel-specific and

16 Sera from 87 TCR-alpha-/- mice were tested for the presence of ANCAs

17 class I MHC molecule H-2Kb, we constructed a TCR alpha chain transgenic mouse in a TCR alpha-deficien

18 cted a TCR alpha chain transgenic mouse in a TCR alpha-deficient background to define specific struct

19 In this study, using a TCR alpha knock-in mouse in which the knock-in alpha-cha

20 ected by the ability to coexpress additional TCR alpha-chains.

21 The human T cells are all TCR alpha beta(+) and display a restricted TCRV beta rep

22 e report that the presence of CD8alpha/alpha TCR-alpha/beta cells in iIELs is independent of classica

23 Thus, the presence of CD8alpha/alpha TCR-alpha/beta cells in iIELs is mainly dependent on the

24 erred from the low numbers of CD8alpha/alpha TCR-alpha/beta T cells in mice deficient in Qa-2 genes.

25 The T cell receptor alpha (TCR alpha) chain is also degraded by a similar set of re

26 However, in both C57BL/6 and TCR alpha transgenic mice, single amino acid replacement

27 L-7(-/-)) mice have reduced numbers of B and TCR alpha beta cells, but lack mature TCR gamma delta ce

28 ral blood GAD65-specific CD4(+) T cells, and TCR alpha-chain next-generation sequencing to bulk memor

29 Thus, the lack of allelic exclusion and TCR alpha secondary rearrangement play a key role in the

30 basis for the loss of the TCR CD3-delta and TCR-alpha subunits from the ER was investigated in lymph

31 ep-sequencing analysis of TCR-beta (TRB) and TCR-alpha (TRA) rearrangements of CD3(-)CD4(+)CD8(-) imm

32 sTCR affinity purified on anti-TCR-alpha and anti-TCR-beta mAb columns had identical CS

33 nalysis of the thymocytes derived from Atm-/-TCR alpha beta+ and control mice suggested that Atm is i

34 of the T-dependent immune responses in Atm-/-TCR alpha beta+ mice indicated that Atm is dispensable f

35 Analysis of the Atm-/-TCR alpha beta+ mice indicated that the transgenic TCR a

36 shown that the development of CD8alpha/beta TCR-alpha/beta cells in lymphoid organs as well as in in

37 Between TCR alpha and Dad1 are eight DNase I hypersensitive site

38 inally, we report that the chromatin between TCR alpha and Dad1 is DNase I hypersensitive in a variet

39 hich TCR delta segments are situated between TCR alpha segments.

40 Both TCR alpha-chains and TCR beta-chains made contact with t

41 notypes with limited TCR gene usage for both TCR alpha- and beta-chains in type II NKT cells reflects

42 fferential J region gene expression for both TCR alpha- and beta-chains.

43 s natural sequence variants of the canonical TCR-alpha and decreased affinity for self/CD1d.

44 All were CD3+, TCR-alpha beta+.

45 Although the presence of all CD8(+)TCR-alpha/beta(1) IELs is dependent on beta2-microglobul

46 The protective IEL appear to be CD8+, TCR-alpha/beta and are at least partially dependent upon

47 iated, FITC-sensitized mice were CD4+, CD8-, TCR-alpha/beta+, MHC restricted, and hapten specific.

48 We applied single-cell TCR alpha- and beta-chain sequencing to peripheral blood

49 CD4, CD8, T-cell receptor alpha/beta chain (TCR alpha beta), and TCR gamma delta.

50 of the T-cell antigen receptor alpha chain (TCR-alpha) in developing thymocytes.

51 high vs low expression of the AI4 clonotypic TCR alpha-chain on developing thymocytes in B6.H2g7 and

52 dilution cloning and analysis of coexpressed TCR alpha-chain genes confirmed that these TCRs were sel

53 reby indicating conservation of the combined TCR alpha delta locus in birds.

54 lls are innate-like T cells with a conserved TCR alpha-chain recognizing bacterial metabolites presen

55 centrally above CD1b, whereby the conserved TCR alpha-chain extensively contacts CD1b and GMM.

56 In contrast, TCR-alpha/beta CD8alpha/alpha cells are found mainly in

57 ed antibodies in the absence of conventional TCR alpha beta+ T cell help.

58 escribes a new method for expressing defined TCR-alpha and TCR-beta proteins from a single 2A peptide

59 We have developed TCR alpha-chain gene-deleted BXSB mice to directly exami

60 ressed both in 293T cells and in 2 different TCR alpha(-)/beta(-) T cell hybridomas.

61 l TCR Vbeta5.1 chains coupled with different TCR alpha-chains.

62 We also identified a diverged TCR-alpha gene segment that uses a divergent recombinati

63 age revealed that the combination of diverse TCR alpha and beta chains could be used for the recognit

64 a single, unique TCR-beta chain and diverse TCR-alpha chains, pinpointing malignant transformation t

65 This reporter gene was excised during TCR alpha-chain VJ-recombination, and the retained H2BeG

66 an increase of IL-4 secretion by CD4(+)DX5(+)TCR alpha beta(+)CD62L(low) T cells but not to increased

67 The variable region of each TCR alpha chain (V alpha) studied to date is found to be

68 g elements: the TCR delta enhancer (Edelta), TCR alpha enhancer (Ealpha), and T early alpha (TEA) pro

69 cDNAs encoding TCR alpha- and beta-chains specific for HLA-A2-restricte

70 equencing the hypervariable regions encoding TCR alpha and beta polypeptides from T cell clones recog

71 pe negative selection by using an endogenous TCR alpha chain created by secondary rearrangement maint

72 ely promoted by, expression of an endogenous TCR alpha-chain.

73 d by analyzing CD8beta levels and endogenous TCR alpha chain rearrangements.

74 expression of both transgenic and endogenous TCR alpha-chains inhibit diabetes transfer.

75 t is based on the knockout of the endogenous TCR alpha and beta genes, followed by the introduction o

76 h, based on the disruption of the endogenous TCR alpha chain only, followed by the transfer of genes

77 creased gene rearrangement at the endogenous TCR alpha locus, or receptor editing.

78 nly with each other but also with endogenous TCR alpha and beta chains, thereby generating alphabetaT

79 r (TCR)-transgenic mice devoid of endogenous TCR-alpha and TCR-beta chains.

80 ic transgenic mice that lack only endogenous TCR-alpha chains developed EAE with high incidence but r

81 alpha+ IEL, although they are deficient for TCR alpha beta+ CD8 alpha beta+ cells.

82 crossing with mice genetically deficient for TCR-alpha, we demonstrate that the T cell help for the a

83 nce of a role for mannosidases was found for TCR-alpha, and significant retrograde movement through t

84 nto the role of class I molecules in forming TCR alpha beta+ CD8+ IEL populations, we have analyzed t

85 Four TCR alpha/beta pairs cloned from single cells of the T c

86 TCR and to shield CD8(-)4(-) thymocytes from TCR alpha beta signals that impair thymocyte proliferati

87 When purified B cells from TCR-alpha-/- mice were mixed with MLN cells before cell

88 er of mesenteric lymph node (MLN) cells from TCR-alpha-/- x Ig mu-/- mice.

89 Administration of the purified Ig from TCR-alpha-/- mice into TCR-alpha-/- x Ig mu-/- mice led

90 Approximately 70% of sera from TCR-alpha-/- mice showed reactivity against human neutro

91 These data indicate that establishing full TCR-alpha LCR activity requires critical molecular event

92 pmental defects in Atm-/- mice, a functional TCR alpha beta transgene was introduced into these mutan

93 n, the appearance of a particular functional TCR alpha chain sequence in cells from mice bearing a tr

94 gonist selection process requires functional TCR alpha-CPM, whereas it is independent of CD8beta expr

95 of specific TCRs and the in vitro functional TCR-alpha- and -beta-chain-pairing assay suggests that e

96 g from unrepaired RAG endonuclease-generated TCR-alpha/delta locus coding ends in primary thymocytes.

97 development of IBD-like lesions in germfree TCR-alpha-deficient mice monoassociated with the protozo

98 development of IBD-like lesions in germfree TCR-alpha-deficient mice.

99 observed in the C. parvum-infected germfree TCR-alpha-deficient mice.

100 Recombination of germline TCR alpha and beta genes generates polypeptide receptors

101 cells with a retrovirus that encodes a given TCR-alpha/beta subunit, TCR-retrogenic mice can be gener

102 We find that K-/-D-/- mice have TCR alpha beta+ CD8 alpha alpha+ IEL, although they are

103 n for diverse Th cells in vivo and highlight TCR alpha-chain dominance in Ag-driven selection for bes

104 confirmed coexpression of highly homologous TCR alpha-chain gene (TCRA) and TCRB sequences.

105 We show that within the human TCR-alpha/delta locus, Ddelta2-Ddelta3 rearrangements oc

106 l killer T (NKT) cells with an invariant (i) TCR alpha chain (iNKT cells) recognize glycolipids from

107 ntigen structure that has a nearly identical TCR alpha chain but a different beta chain, highlighting

108 t subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains.

109 panel of anti-VSV CTL clones with identical TCR alpha-chains.

110 We identified TCR alpha beta pairs in the absence of accessory molecul

111 in and the peptide/MHC complex, we immunized TCR alpha-chain transgenic mice with the VSV peptide and

112 bset of the GFP-expressing cells, whereas in TCR alpha beta cells, endogenous IL-2 is more likely to

113 Rs, and similar motifs were apparent even in TCR-alpha chains of human lupus Th clones.

114 as well as the protection from CF and HF in TCR-alpha(-/-) mice.

115 cells, partially reconstituted CF and HF in TCR-alpha(-/-) recipient mice.

116 etermined in chronic colonic inflammation in TCR-alpha-deficient ((-/-)) mice, in which colitis is me

117 eeded to initiate intestinal inflammation in TCR-alpha-deficient mice.

118 age, and the colitis was more severe than in TCR-alpha-/- mice.

119 ny T cell-specific gene enhancers, including TCR-alpha, TCR-beta, and CD4.

120 ility to function over the large intervening TCR alpha locus and or E delta function requires proximi

121 However, deletion of intervening TCR alpha/delta locus sequences to restore the inserted

122 Transfer of these T cell clones into TCR alpha/beta(-/-) or IFN-gamma(-/-) mice significantly

123 the purified Ig from TCR-alpha-/- mice into TCR-alpha-/- x Ig mu-/- mice led to decrease in the numb

124 Analysis of the mice carrying the introduced TCR alpha chain and the transgenic TCR beta chain from t

125 The introduced TCR alpha chain gene is expressed in a majority of CD3 p

126 e thymocytes from the loss of the introduced TCR alpha chain gene.

127 ficient surface expression of the introduced TCR alpha/beta heterodimer.

128 Invariant TCR alpha chains, self-lipid reactivity, and rapid effec

129 ive natural killer T cells with an invariant TCR alpha-chain (iNKT cells) are a conserved population

130 lymphocytes (IHL) are dominated by invariant TCR alpha-chain expressing CD1d-reactive NKT cells, whic

131 1d-restricted NKT cells expressing invariant TCR alpha-chain rearrangements (iNKT cells) have been re

132 1d-restricted NKT cells expressing invariant TCR alpha-chains (iNKT cells) produce both proinflammato

133 as invariant owing to their mostly invariant TCR alpha-chain usage (Valpha14-Jalpha18 in mice, Valpha

134 ons of natural killer T cells with invariant TCR alpha-chains (iNKT cells) have been identified in mi

135 g evolutionary conservation of the invariant TCR-alpha chain and restricting molecule MR1, this popul

136 mportance of the canonical Valpha14-Jalpha18 TCR alpha chain for antigen recognition by NKT cells is

137 mature T cells expressing Valpha14-Jalpha18 TCR alpha chain in CD1d-deficient mice and studied its r

138 s expressing the invariant Valpha14-Jalpha18 TCR alpha-chain recognize glycolipid Ags such as alpha-g

139 rogression of a rearranged Valpha14-Jalpha18 TCR-alpha chain gene into the Nur77(tg) (Nur77(tg);Valph

140 expressing the invariant Valpha14-Jalpha281 TCR alpha-chain, CD1d1-reactive clones with diverse TCRs

141 e inhibit the dislocation of the full-length TCR alpha chain from the ER, as well as a truncated, mut

142 eristic of CTLper clonotypes despite limited TCR alpha/beta-chain heterogeneity.

143 l peptide epitope was conferred by the lupus TCR-alpha chain even when it paired with a TCR-beta chai

144 The lupus TCR-alpha chains probably contact the nucleosomal peptid

145 raction with MR1, whereas the invariant MAIT TCR alpha chain controlled specificity through a small n

146 s on T cell responses mediated by the mature TCR alpha/beta complex.

147 The mouse TCR alpha-chain gene locus contains a cis-acting locus c

148 e manifestation of all key features of mouse TCR-alpha gene LCR function in T cells derived in vitro

149 n per se because T-cell receptor alpha-null (TCR-alpha(-/-)) and wild-type colon cultures respond sim

150 d a wide frequency range (<0.00001-1.62%) of TCR alpha-chains corresponding to GAD65-specific T cells

151 Assembly of TCR alpha and TCR gamma chain variable region genes exhi

152 contrast, the functional differentiation of TCR alpha beta cells and the development of TCR gamma de

153 y shown that phenotypic allelic exclusion of TCR alpha-chain is functional only in mature thymocytes.

154 Each clone was analyzed for expression of TCR alpha and beta genes by RT-PCR and for target cell s

155 Here, we show that premature expression of TCR alpha beta on early thymocytes curtails thymocyte ex

156 First, the early formation of TCR alpha beta appears to impair the formation and funct

157 ctional consequences of allelic inclusion of TCR alpha-chains, we develop a computational model for t

158 ates, did not promote a significant level of TCR alpha rearrangement beyond that observed in the abse

159 T cells that express intermediate levels of TCR alpha beta molecules (TCRint) and the DX5 Ag (believ

160 These cells express a range of TCR alpha- and beta-chains that show differential recogn

161 his decrease is caused by a rearrangement of TCR alpha chain locus, which deletes the introduced TCR

162 is involved in the expansion/recruitment of TCR alpha beta+ CD8+ IEL following microbial colonizatio

163 und striking similarities in CDR3 regions of TCR alpha and beta chains between our major group of CD8

164 ycoslyation sites in the constant regions of TCR alpha or beta chains could increase the functional a

165 s, we employed next-generation sequencing of TCR alpha-chains that contain the TRAV1-2 gene segment t

166 CR) alpha/beta and that the Vbeta1 subset of TCR alpha/beta T cells had a dominant role in protective

167 The usage of TCR alpha and beta V regions in the cell line was oligoc

168 R, as well as a truncated, mutant version of TCR alpha chain that lacks cysteine residues.

169 r pre-TCR signaling and before completion of TCR-alpha rearrangement.

170 plice site, results in reduced expression of TCR-alpha mRNA.

171 ally correlates with increased expression of TCR-alpha.

172 We also report that de novo introduction of TCR-alpha LCR-linked transgenes into existing T cell lin

173 ion of exogenous TCR-beta chains, but not of TCR-alpha chains, assembly and functional cell surface e

174 hese clones differed only in the N region of TCR-alpha and -beta since the clones had the same Valpha

175 LN and colon and less often in the spleen of TCR-alpha-/- mice with chronic colitis.

176 s (IELs) in mice include two main subsets of TCR-alpha/beta(1) cells which differ functionally and on

177 n in the absence of stimulation and have pre-TCR alpha-chain and CD25 down-regulation, as well as inc

178 ked heterodimer composed of an invariant pre-TCR alpha (pTalpha) subunit and a variable beta subunit,

179 lon, and zeta proteins together with the pre-TCR alpha-chain (pT alpha) and a rearranged TCR beta-cha

180 udes a rearranged TCR beta-chain and the pre-TCR alpha-chain.

181 ptor (TCR) Vbeta gene rearrangements and pre-TCR-alpha expression were normal in ADA-deficient cultur

182 Second, the premature TCR alpha beta contact with intrathymic MHC molecules fu

183 over a long chromosomal distance to promote TCR alpha rearrangement and maximal TCR delta expression

184 ch delay, consequently expressing rearranged TCR alpha beta proteins early in the ontogeny.

185 ypermutation in a nonproductively rearranged TCR alpha-chain gene in a clonal T cell hybridoma that h

186 ding frame in the nonproductively rearranged TCR alpha-chain through the use of cryptic splice sites

187 line of NOD mice that express the rearranged TCR alpha- and beta-chain genes of a diabetogenic NOD CD

188 d from a mouse transgenic for the rearranged TCR alpha- and beta-chains of the D10.G4.1 (D10) Th2 clo

189 lpha) segment of the functionally rearranged TCR-alpha gene was Vdelta5, which was used by seven clon

190 Namely, the iT(reg) TCR alpha-chain and beta-chain are overlaid with the alp

191 High-level, copy number-related TCR-alpha LCR-linked reporter gene expression levels are

192 Using an in vitro system that reproduces TCR alpha enhancer activity efficiently, we show that lo

193 transgenic mice expressing an M3-restricted TCR-alpha/beta from a CD8(+) T cell hybridoma (D7) speci

194 bly, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes

195 ng an important role in maintaining separate TCR alpha and delta loci.

196 hese data argue that thymic selection shapes TCR-alpha V region bias in the preimmune repertoire; how

197 -cells is primarily the function of a shared TCR-alpha chain.

198 retrogenic mice engineered to express single TCR alpha- or beta-chains specific for the D(b)NP366 or

199 This indicates that at least some TCR alpha beta+ CD8 alpha alpha+ IEL require only noncla

200 ntiviral vectors encoding the HIV-1-specific TCR alpha and TCR beta chains cloned from a CTL clone sp

201 MAGE-A3-specific TCR alpha- and beta-chains were isolated and cloned into

202 nsgenic expression of the NK T cell-specific TCR alpha-chain Valpha14Jalpha18 leads to a complete res

203 me A2-restricted cross-reactive NLV-specific TCR-alpha/beta signature (TRAV3TRAJ31_TRBV12-4TRBJ1-1) i

204 we detected T cells with highly stereotyped TCR alpha-chains present among genetically unrelated pat

205 cytes, believed to be caused by premature Tg TCR alpha beta expression via unknown mechanism(s).

206 her in vivo transgene expression levels than TCR alpha beta cells.

207 Here we demonstrate evidence that TCR alpha rearrangement is initiated by rearranging a 3'

208 ide sequence differences of TCR V-genes that TCR alpha chains probably diverged early during evolutio

209 among CD1d-restricted TCRs and suggest that TCR alpha-chain elements contribute to alpha-linked glyc

210 The TCR alpha enhancer (E alpha) located at the 3' end of th

211 The TCR alpha- and beta-chain genes from a tumor-infiltratin

212 The TCR alpha-chain is assembled by somatic recombination of

213 The TCR alpha/delta locus on chromosome 14q contains many V,

214 on synergistically with CREB to activate the TCR alpha enhancer in a chromatin environment.

215 fe than in younger animals, and affected the TCR alpha beta+ CD8+ T cells more than the gamma delta T

216 imultaneous deletion of both E delta and the TCR alpha enhancer (E alpha) demonstrated that residual

217 antly, null mutations in Lck, ZAP70, and the TCR alpha- and beta-chains abrogate Fas signaling.

218 nd generates DNA double-strand breaks at the TCR alpha-chain locus.

219 ions with SNPs over similar distances at the TCR alpha/delta locus.

220 e have developed transgenic mice bearing the TCR alpha- and beta-chains from the BDC-6.9 T-cell clone

221 higher than the beta-chain, and because the TCR alpha-chain V gene segment TRAV1-2 is used by two of

222 Analogous interactions between the TCR alpha-chain and residues in the C-terminal half of t

223 comprehensive interface exchange between the TCR alpha/beta constant domain pair and the IgG1 CH3 hom

224 Using both the TCR alpha- and beta-chains as tweezers to surround and g

225 the type of signal received through both the TCR alpha- and ss-chains.

226 hat immunization of superantigen changes the TCR alpha-chain expression on peripheral superantigen-sp

227 ion to CD8(+) T-cell function, we cloned the TCR alpha and beta chain genes from one effective and tw

228 Consequently, the TCR alpha- and beta-chains form a relatively flat ligand

229 se soluble regulatory factors containing the TCR alpha-chain.

230 for immunization of mice carrying either the TCR alpha- or beta-chain of a VSV8 (unmodified)/H-2K(b)-

231 which then rearrange the locus encoding the TCR alpha-chain (Tcra).

232 transgenic mouse expressing exclusively the TCR alpha-chain from a VSV peptide-specific CD8+ T cell

233 tinal IELs, but only on those expressing the TCR alpha beta receptor and not the TCR gamma delta cell

234 For the TCR alpha chain, both complete ER import and subunit ass

235 cognition, with little contribution from the TCR alpha-chain.

236 transgenic mice with a point mutation in the TCR alpha chain of the D10.G4.1 (D10) TCR and bred them

237 al collaboration of multiple proteins in the TCR alpha enhancer complex.

238 at eight DNase I-hypersensitive sites in the TCR alpha locus comprise an LCR that confers T-cell comp

239 In the TCR alpha-chain, allelic inclusion may be the rule rathe

240 Analysis of CDR3 diversity in the TCR alpha-chains identifies many preferred sequence feat

241 sisted folding at more than one point in the TCR alpha/beta assembly process, which allows specific r

242 Indeed, the TCR alpha subunit restores development of pT alpha-defic

243 vel picornavirus-like 2A peptide to link the TCR alpha- and beta-chains in a single retroviral vector

244 Although tightly linked, the TCR alpha and delta genes are expressed specifically in

245 tal effects, and that it maps in or near the TCR alpha-chain complex, Tcra.

246 Random genomic integration of the TCR alpha and beta chain and expression from nonendogeno

247 Single-cell analysis of the TCR alpha and beta chains showed restricted variable gen

248 ritical for the autonomous regulation of the TCR alpha and Dad1 genes.

249 to the distinct functional capacities of the TCR alpha and delta proteins during thymocyte developmen

250 We found that folding of the TCR alpha chain constant domain Calpha is dependent on a

251 suggest that secondary rearrangements of the TCR alpha chain gene play an important role in the forma

252 We show that degradation of the TCR alpha chain is impaired in the presence of lactacyst

253 hains, and for the observed influence of the TCR alpha chain on SAG reactivity.

254 delta genes are located in the midst of the TCR alpha gene locus.

255 We report, by sequencing of the TCR alpha- and beta-chain associated with CD4(+) Treg, t

256 lementarity-determining regions (CDR) of the TCR alpha- and ss-chains contacting up to five residues

257 quence requirements for CDR1 and CDR2 of the TCR alpha-chain in a human T cell response characterized

258 pmentally regulated allelic exclusion of the TCR alpha-chain is caused by competition between alpha-c

259 The V alpha segment of the TCR alpha-chain is suggested to have a primary role in s

260 Because conservation of the TCR alpha-chain of invariant T cells is much higher than

261 presence of cDNA reverse transcripts of the TCR alpha-chain within the hybridoma, suggesting a role

262 ancer (E alpha) located at the 3' end of the TCR alpha/delta locus functions over a long chromosomal

263 examined LD within an 850 kb section of the TCR alpha/delta locus on chromosome 14q by typing 24 V g

264 urces: a structure-based design study on the TCR alpha chain (nine mutations) and an in vitro selecti

265 The site on the TCR alpha chain responsible for these effects is CDR2.

266 lls more than the gamma delta T cells or the TCR alpha beta+ CD4+CD8- population.

267 ll receptor (TCR) alpha locus and placed the TCR alpha locus enhancer on the derivative 21 chromosome

268 Surprisingly, the TCR alpha beta+ CD8 alpha alpha+ IEL are significantly i

269 We demonstrate that the TCR alpha chain is required for maximum stabilization of

270 SAG-reactive T cells has suggested that the TCR alpha chain may modulate the level of activation thr

271 We find that the TCR alpha promoter is inactivated by a repressor complex

272 is encoded by a gene located adjacent to the TCR alpha and delta genes on mouse chromosome 14.

273 sion on CD8(+) T cell responses, mAbs to the TCR alpha-chain and T cells expressing two TCR species w

274 ngamma subunits were situated underneath the TCR alpha-chain and TCR beta-chain, respectively.

275 e CD3epsilondelta ECDs to sit underneath the TCR alpha-chain.

276 hat proposed for glycopeptides, in which the TCR alpha and beta chains survey a surface composed of b

277 HS1 colocalizes with the TCR alpha enhancer (Ealpha) and is T cell-specific; HS2,

278 3delta chain to be in close contact with the TCR alpha-chain.

279 tical for promoting accessibility within the TCR alpha locus.

280 J recombination and transcription within the TCR alpha/delta locus are regulated by three characteriz

281 The TCR-alpha chains of these murine lupus Th clones shared

282 anslation of the messenger RNAs encoding the TCR-alpha, -beta and CD3-gamma, -delta, -epsilon, and -z

283 We demonstrate that in this cell line the TCR-alpha and -beta chains as well as the CD3gamma, CD3d

284 orientation that prevents interaction of the TCR-alpha chain with the MHC class II beta chain helix.

285 T cells (as determined by expression of the TCR-alpha chain) remained small in size.

286 determinant in the optimal expression of the TCR-alpha chain.

287 ize the ubiquitously active sequences of the TCR-alpha LCR to an 800-bp region containing a prominent

288 globulin (beta2m)-deficient mice, all of the TCR-alpha/beta CD8alpha/alpha and CD8alpha/beta T cells

289 to one CAST genetic trait that mapped to the TCR-alpha locus and led to higher usage of the distal Va

290 We transfected the TCR-alpha and -beta chain genes of a representative, pat

291 and single-positive thymocytes express this TCR alpha chain.

292 increased numbers of circulating and tissue TCR-alpha beta, CD4- CD8- T cells.

293 We find that, similar to TCR alpha and gamma variable region genes, assembly of T

294 T cell maturation was strictly restricted to TCR-alpha beta T cells as the absolute number of thymic

295 pha beta+ mice indicated that the transgenic TCR alpha beta can rescue the defective T cell different

296 abeta and gammadelta T cells can express two TCR alpha or TCR gamma chains, respectively.

297 Using TCR alpha and beta repertoire sequencing for T-cell subs

298 for all the subjects 98% of the T cells were TCR-alpha beta-positive.

299 ndogenous IL-2 RNA upon stimulation, whereas TCR alpha beta cells express more IL-2 than GFP RNA.

300 op EAE spontaneously, T/R+ mice crossed with TCR-alpha and -beta knockout mice developed EAE with the