ライフサイエンスコーパス: TCR-gamma

1 Damage to the TCR gamma-2 chain repertoire and depletion of CD56+ Vgam

2 ductive rearrangement and expression of both TCR gamma and delta genes.

3 ombinatorial diversity of relatively complex TCR gamma and delta loci may contribute to the remarkabl

4 nd HEB permit localized accessibility of the TCR gamma and delta loci to the recombination machinery.

5 and RAG2, each have the ability to activate TCR gamma and delta rearrangement in human kidney cells.

6 Proper TCR gamma and delta V(D)J rearrangement during thymocyte

7 cted a high frequency of clonally rearranged TCR gamma and TCRB genes (17/20 and 15/20 cases, respect

8 J rearrangements within the T cell receptor (TCR) gamma and delta loci.

9 ion of expression of single T cell receptor (TCR) gamma and delta variable (V) region gene segments w

10 However, here we show that both TCR-gamma and -beta recombination intermediates are read

11 ge commitment as a consequence of successful TCR-gamma and -delta gene rearrangement, we do not find

12 c-myc, cis, the T-cell receptor gamma locus (TCR-gamma), and c-pim-1.

13 Northern blot analysis with the TCR gamma cDNA probe revealed 1.9-kb transcripts in the

14 antigens and emphasize the importance of the TCR gamma CDR3 loop and adjacent residues.

15 ugh the regions between CDR2 and CDR3 of the TCR gamma chain and modulated by the affinity of the CDR

16 dependent upon the junctional region of the TCR gamma chain and upon pairing of V gamma 2 and V delt

17 e, we report that the germ-line gene for the TCR gamma chain in a chondrichthyan, the sandbar shark (

18 Assembly of TCR alpha and TCR gamma chain variable region genes exhibit allelic in

19 of TCR-beta chain genes in nine patients and TCR-gamma chain genes in two patients.

20 firmed by expressing a hybrid TCR containing TCR-gamma chain germ-line complementarity determining re

21 equencing platform, we sought to analyze the TCR gamma-chain (TRG) repertoire of gammadelta T cells w

22 ubsets segregated on the basis of use of the TCR gamma-chain or delta-chain indicated the existence o

23 DNA clones with characteristics of mammalian TCR gamma chains, including canonical residues considere

24 madelta T cells can express two TCR alpha or TCR gamma chains, respectively.

25 lls can be divided into subsets based on the TCR gamma-chains they express.

26 This assay detected TCR-gamma clonal GRs in 89 samples (87%) from 36 patient

27 ossibly mediated by STAT5, of the rearranged TCR-gamma complex during development of gammadelta T cel

28 ear regression model integrating whole blood TCR gamma constant region gene expression levels and age

29 TCR gamma constant region gene expression levels and cli

30 f transgene expression in thymic and splenic TCR gamma delta and NKT cells, as well as skin TCR gamma

31 d intestine synthesize IL-7, suggesting that TCR gamma delta cell development could occur in either s

32 Peripheral TCR gamma delta cells accumulate GFP RNA faster than end

33 TCR alpha beta cells and the development of TCR gamma delta cells are partially independent of the T

34 ndogenous IL-2 gene expression in transgenic TCR gamma delta cells may be explained by subset-specifi

35 a(-/-) mice, no development of graft-derived TCR gamma delta cells occurred, indicating that extrathy

36 Donor- and host-derived TCR gamma delta cells were recovered from thymus grafts,

37 TCR gamma delta cells, as well as alpha beta TCR-NKT cel

38 intraepithelial lymphocytes (IEL), including TCR gamma delta cells, can develop extrathymically.

39 In TCR gamma delta cells, IL-2-producing cells are a subset

40 te the role of thymic IL-7 in development of TCR gamma delta cells, newborn TCR beta-deficient (TCR b

41 sing the TCR alpha beta receptor and not the TCR gamma delta cells, which exclusively express CD8 alp

42 IL-7 was required for development of thymic TCR gamma delta cells, while peripheral IL-7 was suffici

43 netics of endogenous IL-2 RNA degradation in TCR gamma delta cells.

44 B and TCR alpha beta cells, but lack mature TCR gamma delta cells.

45 Analysis of TCR gamma delta gene usage in the CNS showed that the on

46 In contrast, TCR gamma delta IEL development occurred efficiently in

47 cating that extrathymic IL-7 did not support TCR gamma delta IEL generation from newborn thymic precu

48 demonstrated that extrathymic development of TCR gamma delta IEL required extrathymic IL-7 production

49 as sufficient for development of extrathymic TCR gamma delta IEL.

50 eric form of CD8 is exclusively expressed on TCR gamma delta IELs and on subsets of NK cells and TCR

51 These results suggest that CD8(+) TCR gamma delta IELs do not require class I MHC for deve

52 These structural requirements of TCR gamma delta recognition of prenyl pyrophosphates dis

53 TCR gamma delta T cells are considered important in the

54 rts describing thymic differentiation in two TCR gamma delta transgenic mouse models have suggested t

55 a/BoyEg mice were found to be susceptible to TCR gamma delta+ cell mediated GVHD-induced lethality ch

56 R gamma delta and NKT cells, as well as skin TCR gamma delta-dendritic epidermal T cells, indicate th

57 eptor alpha/beta chain (TCR alpha beta), and TCR gamma delta.

58 The presence of a subset of Thy1+TCR(gamma delta)IEL was also dependent on CD18 integrins

59 ytic activity and the expression of CD11c by TCR(gamma delta)IEL were up-regulated in the presence of

60 G8 T-cell receptor (TCR) gamma delta transgenic (Tg) mice (specific for the

61 In contrast, TCR-gamma delta + DETC were normal in number and appeara

62 as the absolute number of thymic dendritic, TCR-gamma delta and NK1.1 T cells were equivalent to con

63 Unlike TCR-alpha beta cells, TCR-gamma delta cells express a distinct member of the z

64 uced TCR expression, because intraepithelial TCR-gamma delta cells from the zeta-deficient mice did n

65 In contrast, intraepithelial TCR-gamma delta cells of G8.zeta-/- mice expressed high

66 the role of the Fc epsilon RI gamma-chain in TCR-gamma delta cells, a TCR-gamma delta transgenic mous

67 c epsilon RI gamma-chain associates with the TCR-gamma delta complex in the absence of the zeta-chain

68 d intrathymically, distinguishable by either TCR-gamma delta or -alpha beta surface expression.

69 n RI gamma-chain in TCR-gamma delta cells, a TCR-gamma delta transgenic mouse (G8) has been crossed w

70 These results demonstrate that TCR gamma/delta cells have the capacity to cause acute l

71 To elucidate the potential function of TCR gamma/delta cells in GVHD, we have used transgenic (

72 w transplantation (BMT) confirmed that G8 Tg TCR gamma/delta cells infiltrated GVHD target tissues (s

73 The infusion of G8 Tg (H-2Td) TCR gamma/delta cells into lethally irradiated [900 cGy

74 M CH4, T-cell receptor (TCR) alpha/beta, and TCR gamma/delta constant domain pairs, and we found that

75 -host disease (GVHD) generation, the role of TCR gamma/delta expressing cells in this process has rem

76 ost graft rejecting T cells that express the TCR gamma/delta heterodimer.

77 the rejection process was inhibited by anti-TCR gamma/delta MoAbs.

78 were absent in recipients treated with anti-TCR gamma/delta monoclonal antibodies (MoAbs) but not an

79 GVHD was prevented by the injection of anti-TCR gamma/delta monoclonal antibodies.

80 GVHD target organs are responsible for G8 Tg TCR gamma/delta+ cell mediated lethality.

81 ematopoietic progenitor cells; and (2) donor TCR gamma/delta+ cells can facilitate the alloengraftmen

82 These results show that (1) host TCR gamma/delta+ cells can reject repopulating donor cel

83 In contrast, injection of TCR gamma/delta+ cells into irradiated (900 cGy TBI) B6.

84 o studies have specifically examined whether TCR gamma/delta+ cells might be capable of eliminating B

85 These Tg TCR gamma/delta+ cells respond vigorously to target cell

86 The addition of donor G8 TCR gamma/delta+ cells to TCD donor BM was shown to sign

87 o the latter, one such candidate cell is the TCR gamma/delta+ T cell.

88 Increased numbers of T cell receptor (TCR)-gamma/delta cells have been observed in animal mode

89 Similarly, T cell receptor (TCR)-gamma/delta T cells were not required for maximal i

90 For TCR-gamma/delta (Vgamma2) transgenic mice, nearly all ga

91 The observed protection resulted from TCR-gamma/delta cell-mediated arrest of both viral repli

92 pha and CD8alpha/beta T cells disappear, but TCR-gamma/delta cells are unaffected by the absence of b

93 To define the role of TCR-gamma/delta cells in anti-HSV-1 immunity, TCR-alpha-

94 However, a direct role for TCR-gamma/delta cells in protective immunity for pathoge

95 Most of the TCR-gamma/delta cells in the iIELs also bear CD8alpha/al

96 In both models of HSV-1 infection, TCR-gamma/delta cells limited severe HSV-1-induced epith

97 Thus, this study demonstrates that TCR-gamma/delta cells may play an important regulatory r

98 The demonstration that TCR-gamma/delta cells play an important protective role

99 munity, TCR-alpha-/- mice treated with anti- TCR-gamma/delta monoclonal antibodies or TCR-gamma/delta

100 ast partially dependent upon the presence of TCR-gamma/delta T cells in the host.

101 ti- TCR-gamma/delta monoclonal antibodies or TCR-gamma/delta x TCR-alpha/beta double-deficient mice w

102 o detectable B or T cells (TCR-alpha/beta or TCR-gamma/delta); (2) at least 10-fold lower levels of i

103 mice lack TCR-alpha/beta+ cells but contain TCR-gamma/delta+ cells and a small population of a uniqu

104 D4+ TCR-alpha-/beta+ population and expanded TCR-gamma/delta+ population present in TCR-alpha-/- mice

105 ponsiveness was shown not to require CD8+ or TCR-gamma/delta+ T cells or IFN-gamma.

106 urface alpha/beta T cell receptor alpha/beta(TCR), gamma/deltaTCR, or CD3.

107 All cases with assessable DNA had a TCR gamma gene rearrangement, and lacked Epstein-Barr vi

108 be explained by a defect in T cell receptor (TCR)-gamma gene rearrangement, a defect in TCR-gamma gen

109 obulin (Ig) heavy chain and T-cell receptor (TCR)-gamma gene rearrangements were performed on paraffi

110 IL-7Ralpha(-/-) mice is due to insufficient TCR-gamma gene expression.

111 mma1/Jgamma2 consensus primers were used for TCR-gamma gene rearrangement amplification and PCR produ

112 We conclude that nonradioactive PCR-SSCP for TCR-gamma gene rearrangement analysis is a useful adjunc

113 data supports the model that IL-7R promotes TCR-gamma gene rearrangement by regulating accessibility

114 hibitor trichostatin A released the block of TCR-gamma gene rearrangement.

115 mental potential is not a result of enhanced TCR-gamma gene rearrangement/expression in IL-7R(+) pro-

116 (TCR)-gamma gene rearrangement, a defect in TCR-gamma gene transcription leading to death of gamma/d

117 tive method to detect T-cell receptor gamma (TCR-gamma) gene rearrangements by polymerase chain react

118 ns to generate a diverse repertoire of avian TCR gamma genes early in ontogeny.

119 7 on transcription of endogenous, rearranged TCR-gamma genes in alpha/beta lineage cells.

120 the results show that the poor expression of TCR-gamma genes in IL-7Ralpha(-/-) mice is responsible f

121 sed in IL-7R alpha -/- thymocytes, but these TCR-gamma genes, and Vgamma5, are not transcribed in thy

122 sary for the normal expression of rearranged TCR-gamma genes.

123 say to assess the size and V-family usage of TCR-gamma GRs in 102 concurrent and/or sequential morpho

124 Substitution of the TCR gamma junctional region (N and J) sequences from an

125 nd J) sequences from an Ag-reactive TCR with TCR gamma junctional region sequences from an Ag-nonreac

126 cells to examine the effects of changing the TCR gamma junctional region sequences on reactivity to p

127 t of the TCR beta locus (but did not inhibit TCR gamma locus rearrangement).

128 on C gamma gene were identified in the avian TCR gamma locus.

129 present studies sought to define the chicken TCR gamma locus.

130 n that rearrangement of the T cell receptor (TCR)-gamma locus is virtually abrogated, whereas other r

131 loci, being arrested in the T cell receptor (TCR)-gamma locus, aberrant in the immunoglobulin heavy c

132 ranscription element in the T cell receptor (TCR)-gamma locus, called HsA, between the Vgamma5 and Vg

133 Thus, IL-7 controls recombination at the TCR-gamma locus by regulating locus accessibility.

134 IL-7 signal, we directly tested whether the TCR-gamma locus is accessible to cleavage by recombinant

135 Thus, the failure to rearrange the TCR-gamma locus is due to a failure to initiate cleavage

136 s, supporting a mechanistic role for IL-7 in TCR-gamma locus rearrangement.

137 ding from IL-7Ralpha to rearrangement of the TCR-gamma locus requires the gammac receptor chain and t

138 ignal, no initiation of recombination of the TCR-gamma locus was observed, whereas recombination inte

139 Instead, the TCR-gamma locus was shown to be methylated in IL-7Ralpha

140 Production of sterile transcripts from the TCR-gamma locus, a process that generally precedes rearr

141 , we analyze the recombination defect of the TCR-gamma locus.

142 T cell-specific locus control region for the TCR-gamma locus.

143 Substitution of only the TCR gamma N nucleotide region with that from this Ag-non

144 nduce the appearance of transcripts for cis, TCR-gamma, or c-fos, suggesting a role for Stat5 in thei

145 Four-color TCR-gamma PCR analysis can uncover multiple distinct clo

146 a comprehensive analysis of T cell receptor (TCR) gamma rearrangements in T cell precursors of the mo

147 TCR gamma repertoire diversification was initiated on em

148 rearranged, and sterile Vgamma4 and Vgamma6 TCR-gamma transcripts are expressed in IL-7R alpha -/- t

149 delta cells in these mice, since a high copy TCR-gamma transgene exhibited sufficient residual expres

150 we examined transcription of a prerearranged TCR-gamma transgene in IL-7Ralpha(-/-) mice, as well as

151 The TCR gamma translocon contains at least 5 V region genes,

152 esis, we compared the sequence of rearranged TCR gamma variable region 5 genes in gammadelta+ IEL and

153 PCR showed that TCR gamma variable region 5 was rearranged in gammadelta