ライフサイエンスコーパス: TCRbeta

1 sing the T cell antigen receptor beta-chain (TCRbeta).

2 nd mRNA sequencing of their TCR beta-chains (TCRbeta).

3 splacing cholesterol, which is known to bind TCRbeta.

4 the Vbeta1412-RS with the 3'Dbeta112-RS on a TCRbeta allele lacking Dbeta segments (the Jbeta1(M6) al

5 x on the Jbeta1(omega) allele, an endogenous TCRbeta allele that lacks the Dbeta2-Jbeta2 cluster, cre

6 Vbeta segments on unrearranged TCRbeta alleles are accessible in CD4(-)/CD8(-) (double-

7 ey might be regulated, we analyzed mice with TCRbeta alleles containing preassembled functional Vbeta

8 y, Vbeta14(Rep) recombination also occurs on TCRbeta alleles lacking endogenous Vbeta to DJbeta rearr

9 earrangements occur on only 5% of endogenous TCRbeta alleles, the Vbeta14(Rep) cassette underwent rea

10 machinery cooperate to help enforce IgH and TCRbeta allelic exclusion and indicate that control of V

11 ndogenous VbetaDJbetaCbeta genes can enforce TCRbeta allelic exclusion and reveal another mechanism t

12 To elucidate mechanisms that enforce TCRbeta allelic exclusion in such cells, we analyzed Vbe

13 Thus, TCRbeta allelic exclusion is enforced genetically by the

14 ted grossly normal thymocyte development and TCRbeta allelic exclusion.

15 /lipid complex and high prevalence of Vbeta7 TCRbeta among the CD8(+) iNKT cells strongly point to a

16 TCRbeta analysis of the CD154(+) and CD154(-) fractions

17 In these individuals, T cell receptor beta (TCRbeta) analysis revealed that class II-restricted CD8(

18 We identified 15 TCRbeta and 4 TCRalpha antigen receptor sequences shared

19 TCRbeta and CD3 subunit protein sequence analyses among

20 ell as additional TCRs, consisting of the 2C TCRbeta and endogenous TCRalpha chains.

21 The mandatory requirement of TCRbeta and FcRgamma for FC function provides the first

22 elic expression and V-to-DJ recombination of TCRbeta and IgH genes.

23 tween alleles is more strictly regulated for TCRbeta and IgH loci, we evaluated the ability of ATM to

24 reduced D-to-J and V-to-DJ rearrangements of TCRbeta and IgH loci, whereas Rag2(C/C) mice show decrea

25 have been reported to increase the level of TCRbeta and Igmicro pre-mRNA, suggesting the hypothesis

26 b cells despite the absence of intracellular TCRbeta and reduced IL-7 signaling.

27 habeta T cell development, and by modulating TCRbeta and TCRalpha gene segment utilization.

28 In the present study, screening for TCRbeta and TCRalpha/delta translocations by FISH and li

29 of inter-chromosomal translocations between TCRbeta and TCRdelta D gene segments are also increased

30 stage following successful rearrangement of Tcrbeta, and is triggered by and dependent on concurrent

31 re interpreted in conjunction with TCRgamma, TCRbeta, and TCRdelta rearrangements.

32 Our findings indicate that single TCRbeta are sufficient to confer on TCRalphabeta chains

33 This regulation is not a peculiarity of TCRbeta, as we identified many wild-type genes, includin

34 f gammadelta T cells and CD3(+)CD4(+)CD44(hi)TCRbeta(+)CCR6(+) natural Th17 (nTh17) cells, but not by

35 Cells were found to express TCRalpha, TCRbeta, CD152 (CTLA-4), CD154 (CD40L), T-bet, GATA-3, a

36 a population, BATF-expressing NKT cells are TCRbeta/CD3epsilon(low), but express normal levels of CD

37 imal domains of TCRalpha-CD3deltaepsilon and TCRbeta-CD3gammaepsilon.

38 s transient self-renewal (beta-selection) of TCRbeta(+) CD4(-)CD8(-) double-negative stage 3 (DN3) an

39 of the IEL subpopulations TCRgammadelta(+), TCRbeta(+)CD4(+), TCRbeta(+)CD4(+)CD8alpha(+), and TCRbe

40 ulations TCRgammadelta(+), TCRbeta(+)CD4(+), TCRbeta(+)CD4(+)CD8alpha(+), and TCRbeta(+)CD8alphaalpha

41 mice have greater numbers of IL-17-producing TCRbeta(+)CD4(+)cells in lymphoid organs and in the inte

42 We defined two precursor populations among TCRbeta(+)CD4(-)CD8(-) thymocytes by dependence on the k

43 metry subsequently confirmed the presence of TCRbeta(+) CD8(+) IL-17(+) T cells among tumor-infiltrat

44 a(+)CD4(+), TCRbeta(+)CD4(+)CD8alpha(+), and TCRbeta(+)CD8alphaalpha(+) cells in comparison with wild

45 h classifier, we extracted 4-mers from every TCRbeta CDR3 and represented each 4-mer using biophysico

46 ore, we observed that T cells with identical TCRbeta CDR3 nucleotide sequences were capable of recogn

47 l repertoire formation using high throughput TCRbeta CDR3 sequencing in immunodeficient mice receivin

48 ells expressing a conserved motif within the TCRbeta CDR3.

49 vels of IL-22 and larger numbers of IL-22(+) TCRbeta(+) cells and neutrophils in BALF.

50 ed expansion and enrichment of intracellular TCRbeta(+) cells within the DN population and increased

51 has a restricted TCR repertoire with a fixed TCRbeta chain and a TCRalpha chain minilocus.

52 Thus, transient modulation of the TCRbeta chain by H57-597 mAb exhibits potent, long-lasti

53 cholesterol and sphingomyelin binding to the TCRbeta chain causes TCR dimerization.

54 performed high-throughput sequencing of the TCRbeta chain complementarity-determining region 3 of li

55 we induced a CRISPR/Cas9-mediated KO of the TCRbeta chain in combination with a second-generation re

56 e photocholesterol specifically binds to the TCRbeta chain in vivo.

57 lecular level, we forced the expression of a TCRbeta chain isolated from a peptide-independent allore

58 We purified these cells to generate TCRbeta chain libraries pre-enriched for target antigen

59 The nature and CDR3 loop composition of the TCRbeta chain played a dominant role in determining pMHC

60 TCRbeta chain repertoire of peripheral alphabeta T cells

61 bled during thymocyte development influences TCRbeta chain selection and peripheral Vbeta repertoire.

62 an independent validation, we analysed 5,711 TCRbeta chain sequences from reactive CD4 T cells from 2

63 T cell repertoire as a whole, especially for TCRbeta chain sequences.

64 iple sclerosis, we used high-throughput deep TCRbeta chain sequencing to assess millions of individua

65 understanding of complementary TCRalpha and TCRbeta chain utilization is very limited for pathogen-

66 h peptide were established by the transgenic TCRbeta chain, and that this was compensated by addition

67 Using next-generation sequencing of the TCRbeta chain, clonally expanded T cells as a hallmark f

68 or knowledge of variable region usage in the TCRbeta chain, resulting in a comprehensive, unbiased TC

69 g high throughput parallel sequencing of the TcRbeta chain.

70 transgene-encoded Vbeta5 chain and a revised TCRbeta chain.

71 phenotype and location in mice with a fixed TCRbeta chain.

72 nsferable feature of the peptide-independent TCRbeta chain.

73 xpressed transgene P14 T-cell receptor beta (TCRbeta) chain and CD8beta or did not (WT and KO mice, r

74 Dbeta or Jbeta genes, in place of an intact TCRbeta-chain and in association with TCRalpha.

75 ich is composed of a successfully rearranged TCRbeta-chain and the Pre-Talpha-chain.

76 T cell production in a manner independent of TCRbeta-chain expression.

77 hains in disease pathogenesis and the paired TCRbeta-chain remains unknown.

78 In this study, we used TCRbeta-chain transgenic mice to generate polyclonal nTr

79 encing approach, we determined TCRalpha- and TCRbeta-chain usage, as well as alphabetaTCR pairs expre

80 a tandem, multistep process to quantify rare TCRbeta-chain variable sequences of ASTs in large polycl

81 most cells that fail to produce a functional TCRbeta-chain will die instead of adopting the alternati

82 oded pairwise interactions.Rather, identical TCRbeta chains can have altered peptide-MHC (pMHC) bindi

83 lly increasing the frequency of T cells with TCRbeta chains derived from both Tcrb alleles.

84 Characterizing the TCRalpha and TCRbeta chains expressed by T cells responding to a give

85 ion of transcripts encoding the TCRalpha and TCRbeta chains from single cells.

86 Several TCRbeta chains paired with a transgenic TCRalpha chain t

87 riant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens i

88 sis with paired coexpression of TCRalpha and TCRbeta chains with single-cell resolution.

89 Replacing the germline regions of mouse TCRbeta chains with those of other jawed vertebrates pre

90 pervariable CDR3 regions on the TCRalpha and TCRbeta chains, and obtaining the paired sequences of th

91 When paired with certain Trbv TCRbeta chains, these TCRs recognize lipid antigens pres

92 n CD4(+)Vbeta5(-) T cells expressing revised TCRbeta chains.

93 mplementarity determining region 3 (CDR3) of TCRbeta chains.

94 ls sorted to remove cells bearing endogenous TCRbeta-chains can express newly generated TCRbeta molec

95 Sequence analysis of TCRbeta-chains of IGRP(+) cells reveals the repertoire c

96 with the prerearranged Vbeta in cell surface TCRbeta-chains were observed in Vbeta14(NT) and Vbeta8(T

97 lf of these lymphocytes expressed Vbeta14(+) TCRbeta-chains, even though similar steady-state levels

98 plenic alphabeta T cells expressed Vbeta8(+) TCRbeta-chains, only half of these lymphocytes expressed

99 cells expressed only Vbeta14(+) or Vbeta8(+) TCRbeta-chains, respectively, and lacked Vbeta rearrange

100 ctory aGVHD patients showed a more conserved TCRbeta clonal structure between different biopsy sites

101 HLA-C-restricted, CMV-specific TCRbeta clonotypes dominated the ex vivo T cell response

102 g revealed oligoclonal expansion of specific TCRbeta clonotypes in CD8(+)PD-1(+) compared with CD8(+)

103 body production, and the emergence of public TCRbeta clonotypes in circulating Tfh cells.

104 Furthermore, the most highly expanded TCRbeta clonotypes in the CD8(+) and the CD8(+)PD-1(+) p

105 ic traits of CD8(+) TILs and TCR beta chain (TCRbeta) clonotypic frequency in melanoma tumors to iden

106 We determined the genomic sequence of 244 TCRbeta coding junctions from 112 (63 male, 49 female) s

107 We performed deep sequencing of TCRbeta complementarity-determining region 3 (CDR3) regi

108 We found that T-cell receptor beta (TCRbeta) complementarity-determining region 3 repertoire

109 TCRbeta deep sequencing revealed oligoclonal expansion o

110 T-cell receptor beta (TCRbeta) deep sequencing revealed a striking contraction

111 When mutant SOD1 mice were bred onto a TCRbeta deficient background, disease progression was si

112 cient to induce disease in the resistant B10.TCRbeta(-)/(-)delta(-)/(-) strain.

113 inated by crossing B6x56R with CD4(-/)(-) or TCRbeta(-/-)delta(-/-) mice, and the effects on anti-dsD

114 mmadelta T cell homeostatic proliferation in TCRbeta(-/-)/delta(-/-) mice was not altered in the pres

115 sustained advantage following transfer into TCRbeta(-/-)/delta(-/-) mice, NK1.1(+) gammadelta T cell

116 ing of both immune receptor chains (VH+VL or TCRbeta/delta+TCRalpha/gamma) at the single-cell level f

117 is here using a series of public and private TCRbeta derived from autoimmune encephalomyelitis-associ

118 at patients with OS had marked reductions in TCRbeta diversity compared with control subjects, as exp

119 During this investigation, we focused on TCRbeta(+) DN thymocytes and found that there are at lea

120 least three functionally distinct subsets of TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN

121 nterleukin 7 (IL-7) promotes the survival of TCRbeta(-) DN thymocytes by inducing expression of the p

122 Here we found that IL-7 signaled TCRbeta(+) DN3 and DN4 thymocytes to upregulate genes en

123 Likewise, expression of Bcl-2 in TCRbeta(+) DN3E cells was Gads independent, but Gads was

124 Survival and proliferation of TCRbeta(+) DN3E were independent of Gads, but survival a

125 istinct subsets of TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

126 t of Gads, but survival and proliferation of TCRbeta(+) DN3L cells were Gads dependent.

127 t Gads was necessary for Bcl-2 expression in TCRbeta(+) DN3L cells.

128 f TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

129 n TCRbeta(+) DN4 cells, but proliferation of TCRbeta(+) DN4 cells was Gads dependent.

130 cl-2 expression was not dependent on Gads in TCRbeta(+) DN4 cells, but proliferation of TCRbeta(+) DN

131 cytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

132 -) mice were crossed with Bcl-xL-, Bcl2-, or TCRbeta-expressing transgenic mice, a modest level of co

133 e current study confirms that CD3epsilon and TCRbeta expression are present on the FC at the time of

134 TCRbeta expression in CD4(-)CD8(-) double-negative (DN)

135 a recombination efficiency governs monogenic TCRbeta expression, thereby restraining the expression o

136 encies of ATM-deficient cells with biallelic TCRbeta expression.

137 or related sequences belonging to 17 public TCRbeta families.

138 rs, the cumulative frequency of these public TCRbeta family members was a highly discriminatory indic

139 and establishes that FcRgamma is part of the TCRbeta-FCp33 complex uniquely expressed on FC.

140 on and that FcRgamma coprecipitates with the TCRbeta-FCp33 heterodimer.

141 o results predicted by the accepted model of TCRbeta feedback inhibition, we found that expression of

142 haracterized by a systemic deficit in CD4(+) TCRbeta(+) Foxp3(+) CD25(+) T regulatory cells, increase

143 TCRbeta from patients with RAG mutations had less juncti

144 on of GATA3 leads predominantly to biallelic TCRbeta gene (Tcrb) recombination.

145 pression of either a preassembled functional TCRbeta gene (Vbeta1(NT)) or the prosurvival BCL2 protei

146 Rbeta rearrangements needed for a productive TCRbeta gene further increased frequencies of ATM-defici

147 among T cell progenitors that have completed TCRbeta gene rearrangement without producing a functiona

148 Thymocytes undergoing TCRbeta gene rearrangements are maintained in a low or n

149 ination signal sequences (RSSs) flanking the TCRbeta gene segments.

150 d gender-specific V(D)J recombinase-mediated TCRbeta gene usage and coding joint processing at immune

151 TCRbeta gene usage studies of TRAG-3-specific CD4+ T cel

152 ression of a fully rearranged and functional TCRbeta gene, and most cells that fail to produce a func

153 is study, we demonstrate that a preassembled TCRbeta gene, but not a preassembled DbetaJbeta complex

154 typical B-cell marker, T-cell receptor beta (TCRbeta) gene rearrangement indicated a T-cell origin.

155 the assembly and expression of two distinct TCRbeta genes from a single allele.

156 the CDR3 sequence in millions of rearranged TCRbeta genes from T cells of 2 adults.

157 s method involves sequencing of TCRalpha and TCRbeta genes, and amplifying functional genes character

158 go RAG-dependent rearrangement of endogenous TCRbeta genes, driving surface expression of novel TCRs.

159 xpression of both prerearranged TCRalpha and TCRbeta genes, indicating a critical role for TCR signal

160 phocytes with biallelic expression of IgH or TCRbeta genes.

161 T-cell receptor-beta (TCRbeta) genes naturally acquire premature termination c

162 ling and T-cell antigen receptor beta-chain (TCRbeta) genotyping on sequential genital skin biopsies,

163 ere, we introduce a strictly monovalent anti-TCRbeta H57 Fab' ligand that, when coupled to a supporte

164 vo administration of mAbs specific for mouse TCRbeta (H57-597), TCRalpha or CD3 promptly reduced the

165 TCRbeta high-throughput sequencing in naive CTL of diffe

166 iCD8alpha IEL but not TCRgammadelta(+) IEL, TCRbeta(+) IEL, or intestinal epithelial cells, can prom

167 d developmentally specific V(D)J recombinase TCRbeta immune gene rearrangements and coding joint proc

168 that signaling pathways required to initiate TCRbeta-induced survival and proliferation are distinct

169 Mammalian TCRbeta loci contain 30 Vbeta gene segments upstream and

170 The genomic organization of TCRbeta loci enables Vbeta-to-DJbeta2 rearrangements on

171 the large IgH, Igkappa, TCRalpha/delta, and TCRbeta loci fold into compact structures that place the

172 tes containing unrearranged or prerearranged TCRbeta loci.

173 and lacked Vbeta rearrangements on wild-type TCRbeta loci.

174 We have previously shown that of the two TCRbeta locus (Tcrb) D segments, Dbeta1 is flanked by an

175 -J recombination is outlined using the mouse TCRbeta locus as a model with frequent comparisons to th

176 ocyte development, molecular analyses of the TCRbeta locus in gammadelta cells and the TCRgamma and d

177 s) at which DNA cleavage is defective or how TCRbeta locus sequences contribute to these defects.

178 ps of DNA cleavage by the RAG proteins using TCRbeta locus V, D, and J RSS oligonucleotide substrates

179 sed to drive rearrangement of the endogenous TCRbeta locus, effecting cell rescue through the express

180 e transcription from a Vbeta gene within the TCRbeta locus.

181 sors, accompanied by reduced numbers of both TCRbeta(low) immature single-positive CD8(+) cells and d

182 , resulting in the appearance of CD4(+)CD8(-)TCRbeta(-/low) thymocytes indistinguishable from DP thym

183 Prior studies indicated that public TCRbeta may be preferentially deployed in autoimmunity.

184 Our findings suggest that TCRbeta-mediated feedback inhibition of Vbeta14 rearrang

185 1(omega) alleles were similarly regulated by TCRbeta-mediated feedback regulation.

186 In the absence of alphabeta T cells, TCRbeta(-/-) mice exposed to S. rectivirgula for 4 wk ha

187 TCRbeta(-/-) mice were highly susceptible to T cell-medi

188 delta T cells by crossing LATY136F mice with TCRbeta(-/-) mice.

189 were reduced in spinal cords of SOD1(G93A) (TCRbeta-/-) mice.

190 We hypothesized that if these TCRbeta modulate the likelihood of a TCRalphabeta hetero

191 that three of the identified CMV-associated TCRbeta molecules bind CMV in vitro, and, moreover, we u

192 s TCRbeta-chains can express newly generated TCRbeta molecules in adoptive hosts.

193 NMD but instead reflects retention of PTC(+) TCRbeta mRNA in the nuclear fraction of cells.

194 Analysis of TCRbeta mRNA kinetics after either transcriptional repre

195 n-gamma-producing NKT-like (CD1d-independent TCRbeta+,NK1.1+ and/or DX5+) cells.

196 cers were identified as predominantly DX5(+) TCRbeta(+) NKT cells, and a comparable response could be

197 tion of the NMD factor UPF3b does not impair TCRbeta NMD, thereby distinguishing it from classical NM

198 ombination with UPF3b, also has no effect on TCRbeta NMD.

199 The frequency of TCRbeta nucleotide sequences was significantly higher in

200 Mice lacking alphabeta T cells (TCRbeta(null)) were completely deficient in their abilit

201 ease the frequencies of cells with biallelic TCRbeta or IgH expression while decreasing the frequency

202 ression or aberrant V-to-DJ rearrangement of TCRbeta or IgH loci in mice lacking ATM.

203 TCRbeta or TCRalphabeta transgenes failed to rescue DNMA

204 evels in mice lacking mast cells or T cells (TCRbeta(-/-) or Rag1(-/-)).

205 oglobulin heavy, T-cell receptor (TCR)alpha, TCRbeta, or TCRgamma chains expressed in a population of

206 public, but not private, disease-associated TCRbeta paired with endogenously rearranged TCRalpha end

207 t a hydrophobic patch created after TCRalpha-TCRbeta pairing has a role in maintaining the conformati

208 etains information about individual TCRalpha-TCRbeta pairs, TCRs of interest can be expressed and use

209 s us to track type II NTK cells by the GFP(+)TCRbeta(+) phenotype in the thymus and liver.

210 K/Akt pathway, which is required for pTalpha/TCRbeta (pre-TCR)-induced survival, differentiation, and

211 ich cells expressing functionally rearranged TCRbeta proliferate and differentiate into CD4(+)CD8(+)

212 rearrangement without producing a functional TCRbeta protein.

213 e of the two alleles predicting a functional TCRbeta protein.

214 IgH and TCRbeta proteins drive proliferation of prolymphocytes t

215 two of six public, but none of five private TCRbeta provoked spontaneous early-onset autoimmunity in

216 ision, RAG reexpression mediates extrathymic TCRbeta rearrangement and results in a population of pos

217 the Pten gene prior to the formation of the TCRbeta rearrangement, produced early in development.

218 apoptosis were able to undergo a successful TCRbeta rearrangement, they exhibited a highly abnormal

219 ith clonal TCRalpha but no comparable clonal TCRbeta rearrangement, yielding events that would not no

220 production from progenitor cells undergoing TCRbeta rearrangement.

221 gammadelta lineage occurred before complete TCRbeta rearrangements in most cases.

222 subject to feedback inhibition, we analyzed TCRbeta rearrangements in Vbeta14(Rep) mice containing a

223 DJbeta complex that decreases the number of TCRbeta rearrangements needed for a productive TCRbeta g

224 idiopathic CP and a positive correlation of TCRbeta rearrangements with disease severity scores.

225 ifferentiation of thymocytes with productive TCRbeta rearrangements.

226 We find that total TCRbeta receptor diversity is at least 4-fold higher tha

227 ly devised assay, we characterized 48 unique TCRbeta recombination signal sequence (RSS) end insertio

228 Dicer promotes survival of cells attempting TCRbeta recombination.

229 ncing DNA double-strand breaks (DSBs) during TCRbeta recombination.

230 equencing revealed a significant increase in TCRbeta repertoire diversity and reduced clonality in bo

231 several primary recipients to increase their TCRbeta repertoire diversity.

232 y, we systematically analyzed changes of the TCRbeta repertoire driven by EAE and pregnancy using TCR

233 comprehensive evaluation of the naive CD8(+) TCRbeta repertoire in mice.

234 nt representation of a highly diverse public TCRbeta repertoire in the disease response.

235 Preselection-Tcrbeta repertoire is impaired and antigen-specific IgG

236 antify where limitations imposed on the Treg TCRbeta repertoire results in a population of Tregs that

237 In this study, we have employed deep TCRbeta repertoire sequencing with normalization based o

238 About 8-11% of the Treg TCRbeta repertoire was estimated to be the minimum requi

239 In this study, the Treg TCRbeta repertoire was reshaped and further narrowed.

240 ection of CD8alphaalpha precursors and their TCRbeta repertoire, but not in the maintenance of CD8alp

241 nts on the same allele can contribute to the TCRbeta repertoire.

242 phaalpha precursors and limits their private TCRbeta repertoire.

243 us to investigate the T cell receptor beta (TCRbeta) repertoire in the CP and control groups.

244 echanisms for ensuring generation of diverse TCRbeta repertoires.

245 scovered a substantial number of public CDR3-TCRbeta segments that were identical in mice and humans.

246 ell development at the T cell receptor beta (TCRbeta) selection checkpoint and during positive select

247 lyzed sharing and similarity of CMV-specific TCRbeta sequences and identified 63 public or related se

248 on events in different cells, while abundant TCRbeta sequences are primarily derived from large clone

249 Using a chimeric gene that contains TCRbeta sequences conferring this upregulatory response,

250 We identified TCRbeta sequences crucial for NIPS but found that NIPS i

251 We used GLIPH2 to analyze 19,044 unique TCRbeta sequences from 58 individuals latently infected

252 sequencing was used to identify >18 x 10(6) TCRbeta sequences from the CNSs, periphery, and thymi of

253 can be used to capture and pair TCRalpha and TCRbeta sequences from total T-cell RNA, enabling revers

254 In this study, we assessed TCRalpha and TCRbeta sequences of mouse tTreg and thymic conventional

255 Mapping experiments revealed the identity of TCRbeta sequences that elicit a switch to UPF3b dependen

256 f TCRbeta transcripts, and we identified non-TCRbeta sequences that elicit NIPS.

257 implicate positive selection for promiscuous TCRbeta sequences that likely evade negative selection,

258 sequencing data, we found that abundant CDR3-TCRbeta sequences were clustered within networks generat

259 ments of the naive CD8(+) T-cell repertoire, TCRbeta sequences with convergent features were (i) pres

260 ose CMV status from the resulting catalog of TCRbeta sequences with high specificity and sensitivity

261 -ligands, in the abundance of "public" human TCRbeta sequences.

262 , we identified a total of 1052 CMV-specific TCRbeta sequences.

263 ng a combination approach of high-throughput TCRbeta sequencing and multiparametric flow cytometry, w

264 TCRbeta sequencing revealed a significant increase in TC

265 s using fluorescence-activated cell sorting, TCRbeta sequencing, and RNA-Seq, in reactive and hyporea

266 cells was analyzed at various ages employing TCRbeta sequencing.

267 is study, we performed T cell receptor beta (TCRbeta) sequencing of virus-specific CD8 T cells during

268 When this gene is in-frame, Trbv5 evades TCRbeta-signaled feedback inhibition and recombines by i

269 d on these findings, we propose CMV-specific TCRbeta signatures as a biomarker for an antiviral T cel

270 In this study, we use a fixed TCRbeta system to show that the TCR repertoire of the Fo

271 Although postrevision CD4(+)Vbeta5(-)TCRbeta(+) T cells accumulate with age in Vbeta5 transge

272 results in the appearance of CD4(+)Vbeta5(-)TCRbeta(+) T cells, coinciding with Rag1, Rag2, and TdT

273 n occur at three T-cell receptor (TCR) loci: TCRbeta, TCRgamma and TCRdelta.

274 t are completely devoid of T cells (B6.129P2-Tcrbeta(tm1Mom) Tcrdelta(tm1Mom)/J) show protection agai

275 lar TCR-beta protein and decreased levels of tcrbeta transcript are expressed by T cells cultured in

276 sites up-regulated an alternatively spliced TCRbeta transcript that skipped the mutations independen

277 t up-regulation of the alternatively spliced TCRbeta transcript.

278 PTC-bearing TCRbeta transcripts are dramatically down-regulated to p

279 smic fraction mRNA ratio that results in few TCRbeta transcripts escaping to the cytoplasmic fraction

280 d that NIPS is not exclusively a property of TCRbeta transcripts, and we identified non-TCRbeta seque

281 dominant clone varied between 11% and 99% of TCRbeta transcripts.

282 repertoire breadth to a non-self-antigen, a TCRbeta transgenic mouse model (EF4.1) expressing a limi

283 pertoire of T(reg) cells in Foxp3-sufficient TCRbeta-transgenic mice, suggesting that these self-reac

284 cluding radial chromosome translocations and TCRbeta translocations, compared with cells lacking Atm

285 urther insight into this question, we used a TCRbeta transmembrane domain mutant model that is defect

286 Here we show that cholesterol bound to the TCRbeta transmembrane region keeps the TCR in a resting,

287 l receptor (TCR) chains, TCRalpha (TRAC) and TCRbeta (TRBC), were deleted in T cells to reduce TCR mi

288 ffector memory-RA(+) subsets with restricted TCRbeta usage and nearly monoclonal CDR3 containing nove

289 that FOXP3(+) Tregs possess highly exclusive TCRbeta usage from conventional T cells, in blood, and a

290 We studied the T-cell receptor beta-chain (TCRbeta) usage and phenotypes of peanut-activated, CD154

291 tepwise assembly and subsequent selection of TCRbeta V region exons during thymocyte development.

292 ombined the DNA of one T cell receptor beta (TCRbeta) V-to-DJ-joined allele in a functional configura

293 d/or TCRgamma rearrangements but no complete TCRbeta variable diversity joining rearrangement in surf

294 ghly biased, with a predominant usage of the TCRbeta variable gene 2 (TRBV2) in vaccinees as well as

295 TCRbeta variable gene segments remained largely in germl

296 can assemble with both chicken TCRalpha and TCRbeta via conserved polar transmembrane sites.

297 nd, consistently, public, disease-associated TCRbeta were observed to be commonly oligoclonal.

298 A tilting of the pre-TCRbeta when bound to the pMHC ligand recognition surfac

299 drive oncogene expression differ markedly in TCRbeta (which are exclusively enhancer driven) and TCRa

300 Using T-cell receptor-beta (TCRbeta), which naturally acquires PTCs at high frequenc