ライフサイエンスコーパス: TCRdelta

1 alyzed in mice that lack gammadelta T cells (TCRdelta(-/-)).

2 l receptor (TCR) loci: TCRbeta, TCRgamma and TCRdelta.

3 In the subsequent CD44lowCD25+ stage, most TCRdelta alleles are fully recombined, whereas TCRbeta r

4 we show that approximately half of the total TCRdelta alleles initiate rearrangements at the CD44high

5 egments rearranged to other gene segments of TCRdelta and alpha.

6 no gross deficiency in rearrangements of the TCRdelta and certain gamma loci in pre-T cells, and a fu

7 ns of TCRgamma genes occur concurrently with TCRdelta and D-Jbeta rearrangements, but before Vbeta ge

8 velopmental stage-specific activities of the TCRdelta and TCRalpha enhancers (Edelta and Ealpha), res

9 ias toward productive rearrangements of both TCRdelta and TCRgamma genes among CD44highCD25+ thymocyt

10 , as defined by expression of TCRgammadelta, TCRdelta and/or TCRgamma rearrangements but no complete

11 FSTL-1 Hypo/TCRdelta(-/-) and FSTL-1 Hypo/IL17ra(-/-) were also gene

12 responses to NP and flagellin in Tcrbeta(-/-)Tcrdelta(-/-) and NSG/B mice.

13 in similar levels of IL-17A in the lungs of TCRdelta(-/-) and wild-type C57BL/6 mice.

14 gammadelta cell-deficient mice (TCRdelta(-/-)) and KGF-deficient mice (KGF(-/-)), but no

15 Functional blocking anti-TCRdelta antibody was administered therapeutically, and

16 d restriction on the variable gene usage for TCRdelta assembly.

17 ark IgH clusters, and a novel lineage in the TCRdelta-associated Ig-like V segments.

18 This distinct lineage of TCRdelta-associated IgH-like V segments was termed "TAIL

19 -like rearrangements we found expressed with TCRdelta (but not IgH) rearrangements in our phylogeneti

20 coded by TRAV and TRAJ segments and those of TCRdelta by TRDV, TRDD, and TRDJ segments.

21 aratope structure; additionally, nurse shark TCRdelta CDR3 are more similar to IgH CDR3 in length and

22 D8+ thymocytes, and constituted up to 10% of TCRdelta+ cells in lymphoid organs.

23 ement in CD4(-)CD8(-) thymocytes to form the TCRdelta chain of the gammadelta TCR and V-Jalpha rearra

24 ed by the affinity of the CDR3 region of the TCRdelta chain, which was phosphoantigen independent (pA

25 functions with TCRdelta promoters to mediate TCRdelta-chain gene assembly in DN thymocytes.

26 TCRdelta-chain genes are assembled in CD4(-)CD8(-) (doub

27 mmadelta T-cell receptor (TCR) incorporating TCRdelta-chain variable-region-2 [Vdelta2((+))], which a

28 oration of D segments into IgH, TCRbeta, and TCRdelta chains also contributes to junctional diversifi

29 nctionally rearranged TCRbeta, TCRgamma, and TCRdelta chains by means of transgenes.

30 ose of mice transgenic for both TCRgamma and TCRdelta chains, and numbers of alphabeta thymocytes sim

31 lates strongly with a somatically recombined TCRdelta complementarity-determining region 3 (CDR3) mot

32 )/(-) females was actually higher than in B6.TCRdelta(-)/(-) controls.

33 po and reduced lung burden compared with the TCRdelta(-/-) controls.

34 romosomal translocations between TCRbeta and TCRdelta D gene segments are also increased in the core

35 The mouse TCRalpha/TCRdelta/Dad1 gene locus bears a locus control region (L

36 T-cell receptor delta (TCRdelta)-deficient mice showed a decrease in IL-17 prod

37 In contrast to wild-type mice, WD-fed TCRdelta-deficient and CCR6-deficient mice had reduced s

38 Strikingly, the lack of gammadelta TILs in TCRdelta-deficient but also in CCR2-deficient mice enhan

39 Analysis of TCRdelta-deficient mice indicated that gammadelta T cell

40 rmal hyperplasia in Il23r(-/-)Rag1(-/-)- and Tcrdelta-deficient mice, which can be prevented by neutr

41 FSTL-1 Hypo/TCRdelta(-/-) displayed a lung burden similar to that of

42 F-mediated chromatin loop directly regulated TCRdelta diversity and indirectly regulated TCRalpha div

43 in chicken and turkey is reminiscent of the TCRdelta duplication that is present in nonplacental mam

44 Here we report that deletion of the TCRdelta enhancer (Edelta), which initiates TCRdelta rea

45 s contribute to spontaneous keratitis in B10.TCRdelta(-)/(-) female mice, nor does it appear to depen

46 ice have dry eyes compared with resistant B6.TCRdelta(-)/(-) females and also rederived the B10.TCRde

47 Tear production in B10.TCRdelta(-)/(-) females was actually higher than in B6.T

48 om wild-type donors reduced keratitis in B10.TCRdelta(-)/(-) females.

49 did not significantly reduce disease in B10.TCRdelta(-)/(-) females.

50 s, only a few are predominantly used for the TCRdelta gene assembly, while most are for TCRalpha.

51 The status of TCRdelta gene rearrangements in the remaining alphabeta-

52 several in-frame VDJdelta rearrangements in TCRdelta gene-deficient mice are strikingly underreprese

53 This variation is prominent in TCRdelta gene-deficient mice but is also detectable in w

54 n contrast, the T-cell receptor delta chain (TCRdelta) gene is silent in human prostate.

55 Assembly of TCRalpha and TCRdelta genes from the TCRalpha/delta locus is tightly

56 n the rearrangement of endogenous Vdelta7(+) TCRdelta genes, which paired with the Vgamma2(+) TCRgamm

57 Nurse shark TCRdelta have long CDR3 loops compared with the other th

58 ssed with TCRgamma, canonically found in the TCRdelta heterodimer.

59 The configuration of the second TCRdelta in chicken and turkey is reminiscent of the TCR

60 rganization suggests an origin of the second TCRdelta in the former lineage involving gene duplicatio

61 The second TCRdelta is not found in another avian lineage, the pass

62 The repertoire of TCRdelta is polyclonal in all subsets, indicating that t

63 s features analogous to a recently described TCRdelta isoform in sharks.

64 D genes in the TCRbeta and TCRdelta loci are flanked by a 12RS and 23RS, and their

65 whereas focusing occurred at the TCRbeta and TCRdelta loci, including some TCRbeta sequence-sharing,

66 nformation is available for the TCRgamma and TCRdelta loci.

67 y associated with V(D)J recombination at the TCRdelta locus as the molecular origin of both lymphocyt

68 e results indicate that rearrangement at the TCRdelta locus can precede that of TCRbeta locus recombi

69 nged with a random productivity profile; the TCRdelta locus contained primarily nonproductive rearran

70 osome sequencing approach and found that the TCRdelta locus houses a complex of V segments from multi

71 open Jdelta1 and Ddelta2 coding ends at the TCRdelta locus in SCID thymocytes.

72 an genomes revealed the presence of a second TCRdelta locus in the Galliformes.

73 The TCRdelta locus is contained within the TCRalpha locus; T

74 This second TCRdelta locus is nonsyntenic to the conventional TCRalp

75 In this article, we report that in the TCRdelta locus, the Rag proteins are not the major deter

76 Confusing these observations, FSTL-1 Hypo/TCRdelta(-/-) lungs had an increased percentage of IL-17

77 The study tested whether susceptible B10.TCRdelta(-)/(-) mice have dry eyes compared with resista

78 in the absence of Vgamma1(+) T cells in B10.TCRdelta(-)/(-) mice may be insufficiently checked to pr

79 Rederived B10.TCRdelta(-)/(-) mice still developed keratitis.

80 d perivascular adipose tissue was blunted in Tcrdelta(-/-) mice (P<0.01).

81 d-type mice, both of which were abrogated in Tcrdelta(-/-) mice (P<0.01).

82 TCRdelta(-/-) mice also exhibited >60% reduction in plat

83 The cytokine response in the brain of TCRdelta(-/-) mice appears to be a mixed type1/type 2 re

84 TCRdelta(-/-) mice are characterized by reduced inflamma

85 swelling is reduced by approximately 50% in TCRdelta(-/-) mice compared with wild-type mice.

86 Immunopathological studies indicated that TCRdelta(-/-) mice develop little inflammatory response

87 Consistent with this, TCRdelta(-/-) mice did not develop oral tolerance upon o

88 TCRdelta(-/-) mice exhibited reduced inflammation and de

89 However, FSTL-1 Hypo/TCRdelta(-/-) mice had greater bacterial dissemination t

90 to be devoid of any DETC, we discovered that TCRdelta(-/-) mice have alphabeta TCR-expressing DETC wi

91 TCRdelta(-/-) mice have elevated viral loads and greater

92 l proliferation in wild-type mice but not in TCRdelta(-/-) mice or KGF(-/-) mice.

93 BL/6 (B6) wild-type, B6 TCRbeta(-/-), and B6 TCRdelta(-/-) mice received anti-CD4 and anti-CD8 mAbs,

94 TCRdelta(-/-) mice receiving a single irr-spz immunizati

95 r dermal gammadeltaT cell reconstitution and TCRdelta(-/-) mice reconstituted with Vgamma6 develop ps

96 Adoptive transfer of gammadelta T cells to TCRdelta(-/-) mice reduced the susceptibility of these m

97 Sporozoite-challenged TCRdelta(-/-) mice showed a significant (P < 0.01) incre

98 Approximately 15% of TCRdelta(-/-) mice survived primary infection with WN vi

99 A greater percentage of TCRdelta(-/-) mice than of immunocompetent mice progress

100 In contrast, transfer of CD8(+) T cells from TCRdelta(-/-) mice that survived primary challenge with

101 stored neutrophil and platelet influx in the TCRdelta(-/-) mice to wild-type levels and increased CXC

102 merism was enhanced in both TCRbeta(-/-) and TCRdelta(-/-) mice treated with anti-CD4, anti-CD8, and

103 Increased rat chimerism was observed in TCRdelta(-/-) mice treated with anti-CD4, anti-CD8, and

104 In support of this result, TCRdelta(-/-) mice were also found to be more susceptibl

105 Treatment of TCRdelta(-/-) mice with rIL-22 significantly promoted wo

106 ossed with gammadelta T cell-deficient mice (TCRdelta(-/-) mice) yielding TRAMP x TCRdelta(-/-) mice,

107 t mice (TCRdelta(-/-) mice) yielding TRAMP x TCRdelta(-/-) mice, a proportion of which developed more

108 In Tcrbeta(-/-)Tcrdelta(-/-) mice, linked coengagement of TLR4-BCR by L

109 In TCRdelta(-/-) mice, Mesocestoides corti metacestodes pre

110 Male C57BL/6 wild-type and Tcrdelta(-/-) mice, which are devoid of gammadelta T cel

111 ely transferred into intracranially infected TCRdelta(-/-) mice.

112 hanced by anti-Thy1.2 and anti-NK1.1 mAbs in TCRdelta(-/-) mice.

113 IL-17A-producing cells compared with that of TCRdelta(-/-) mice.

114 gammadelta T-cell-deficient (TCRdelta(-/-)) mice have significantly reduced inflammat

115 gammadelta T-cell-deficient (TCRdelta(-/-)) mice on a C57BL/6 background were challen

116 stinal lamina propria and Peyer's patches of TCRdelta-/- mice compared with the orally immunized cont

117 In contrast, double-positive thymocytes from TCRdelta-/- mice display random proportions of TCRgamma

118 The TCRdelta-/- mice produced much lower levels of IgA antib

119 reconstitute the barrier function defect in TCRdelta-/- mice, while Vgamma5-/- mice also show enviro

120 , and fecal samples were markedly reduced in TCRdelta-/- mice.

121 es in gamma/delta T cell receptor-deficient (TCRdelta-/-) mice versus control mice of the same geneti

122 activation in vivo, we analyzed DETC in the TCRdelta(-/-) mouse.

123 Mice deficient in gammadelta T cells (TCRdelta(-/-)) or wild-type mice treated systemically wi

124 Edelta functions with TCRdelta promoters to mediate TCRdelta-chain gene assemb

125 The defective TCRdelta rearrangement of the 129-"Vdelta7" gene was ass

126 TCRdelta enhancer (Edelta), which initiates TCRdelta rearrangement, significantly improves alphabeta

127 Furthermore, the Ig-TCRdelta rearrangements are expressed with TCRgamma, can

128 among a growing number of taxa employing Ig-TCRdelta rearrangements that blend these distinct lineag

129 characteristics highly similar to canonical TCRdelta rearrangements.

130 d in conjunction with TCRgamma, TCRbeta, and TCRdelta rearrangements.

131 Those that dominate the adult TCRdelta repertoire are hyperacetylated in DN thymocytes

132 rmed "TAILVs." Our data illustrate a dynamic TCRdelta repertoire employing TCRdeltaVs, NARTCRVs, bona

133 Analysis of the nurse shark Ig-TCRdelta repertoire found that these rearrangements poss

134 ratified and essentially public, whereas the TCRdelta repertoire shows much higher levels of clonal d

135 The TCRdelta repertoire, in contrast, was highly skewed in t

136 followed as a consequence of the restricted TCRdelta repertoire.

137 e to D and J segments and dominate the adult TCRdelta repertoire.

138 ments contributes substantially to the adult TCRdelta repertoire.

139 uble-positive thymocytes to generate diverse TCRdelta repertoires and TCRalpha repertoires, respectiv

140 equencing approach to study the TCRgamma and TCRdelta repertoires of naive ex vivo PBMC, Leptospira-r

141 Unlike previous reports in which the TCRdelta(-/-) skin was found to be devoid of any DETC, w

142 ta(-)/(-) females and also rederived the B10.TCRdelta(-)/(-) strain to test for the role of an infect

143 portant gene loci, including one between the TCRdelta/TCRalpha gene segments and the ubiquitously exp

144 chain, and its size was severely reduced in TCRdelta(-/-) Tg-gammadelta mice.

145 tors, and platypus, that can use an atypical TCRdelta that appears to be a chimera of a TCR chain wit

146 devoid of T cells (B6.129P2-Tcrbeta(tm1Mom) Tcrdelta(tm1Mom)/J) show protection against pathogenic c

147 We demonstrate that the lack of TCRgamma and TCRdelta transcripts in the skink are due to large delet

148 Expression of these atypical TCRdelta transcripts with a VH domain paired with Cdelta

149 ke V segment, nestled within the nurse shark TCRdelta translocus, grouped with IgHV-like rearrangemen

150 among F4/80-expressing macrophages and among TCRdelta+ unconventional T cells.

151 the activation status in blood, the type of TCRdelta variant used in blood, and small but significan