ライフサイエンスコーパス: TFP

1 TFP also act as external sensor to regulate EPS producti

2 TFP are filamentous surface appendages primarily compose

3 TFP are known to bind to secreted exopolysaccharides (EP

4 TFP can also pause for extended periods between two exte

5 TFP causes loss of radiation-induced Nanog mRNA expressi

6 TFP deficiency causes a Reye-like syndrome, cardiomyopat

7 TFP did not alter the intrinsic radiation sensitivity of

8 TFP+AL together were completely ineffective in preservin

9 TFP-deficient M. catarrhalis bacteria exhibit diminished

10 TFP-dependent gliding motility has been seen in many Gra

11 abeled with a thiol-reactive derivative of 6-TFP and the spectra were recorded as a function of vario

12 ridone and 6-(trifluoromethyl)-2-pyridone (6-TFP) displayed the greatest sensitivity of the monofluor

13 We predict a TFP retraction speed for the tracking data we study that

14 injury in the NHBD than MPS alone, AL, or AL+TFP.

15 that surface sensing may be widespread among TFP-expressing bacteria.

16 inhibitor saracatinib reduces both basal and TFP-induced erbB3 and calmodulin-dependent protein kinas

17 nary structural relationship between DIF and TFP using double fixed-effects models combined with robu

18 mplex nonlinear relationship between DIF and TFP.

19 PilO and PilN act similarly in flagella and TFP by differentially regulating motility and microcolon

20 including T3SS, exotoxin A, protease IV, and TFP.

21 OF films of COF-PEO-3 (hydrazone-linked) and TFP-PEO-3 (beta-ketoenamine-linked), revealed a Young's

22 t a surface with low levels of piliation and TFP activity, which both progressively increase as the c

23 the proteolytic cleavage site between PR and TFP revealed the presence of an additional site and the

24 ence of a p1 peptide separating protease and TFP.

25 elationship between cAMP, the Chp system and TFP regulation.

26 To assess TFP diversity in Gram-positive bacteria and facilitate p

27 With this IDO inhibitor-assisted TFP system we observed efficient inhibition of primary a

28 inhibition of S100A4 function occurs only at TFP concentrations that promote S100A4 oligomerization.

29 similar to those found in the two bacterial TFPs.

30 To explore the relationship between TFP and DIF, we first applied traditional double fixed-e

31 s of expansion medium supplementation (bFGF, TFP, FBS) and self-assembled construct seeding density (

32 (TFP) on solid surfaces, which requires both TFP and exopolysaccharides (EPS).

33 rs and that biofilm formation is enhanced by TFP expression.

34 tter, levels of inhibition by 125-C9 than by TFP and W-13.

35 volved in the biosynthesis of M. catarrhalis TFP and determined that the TFP expressed by this organi

36 ATPase activity of the TFP motors to change TFP extension and retraction velocities and PilA concent

37 Combined, TFP and Low density interact to further enhance construc

38 , but are instead consistent with a constant TFP-generated force interacting with EPS, which function

39 On surface contact, TFP retraction activates the Chp chemosensory system pho

40 pilins, including 214 in operons containing TFP biosynthetic genes (TBG+ operons).

41 endent transcriptional regulator Vfr control TFP biogenesis gene expression, we explored the relation

42 We discovered that the Chp system controls TFP production through modulation of cAMP while control

43 uation of a new phenazine-based 2D COF (DAPH-TFP COF), as well as its composite with poly(3,4-ethylen

44 Devices containing DAPH-TFP COF were able to deliver both high-energy and high-p

45 The unmodified DAPH-TFP COF exhibited excellent electrical access to its red

46 In cells with Ca(2+) stores depleted, TFP further increased CSQ1 monomerization and CSQ1-STIM1

47 that loading of either monomeric or dimeric TFP cargo occurs with unprecedented high fidelity and ex

48 Molecular motors drive TFP extension and retraction, but whether and how these

49 membrane and a loss/gain of monomers during TFP extension/retraction produces substrate stiffness-de

50 ther bacterial species simultaneously encode TFP, the Chp system, FimL, FimV and adenylate cyclase ho

51 Genes encoding TFP are also found in the genomes of all nine Clostridiu

52 a promising dispersal agent, and establishes TFP inhibition as a viable mechanism for P. aeruginosa d

53 Extended TFP that are composed of thousands of copies of PilA ret

54 We delineated the molecular basis for TFP deficiency in two patients with a unique phenotype c

55 Here, we build a simple model for TFP-driven surface motility without complications from v

56 While PilG phosphorylation is required for TFP function and mechanochemical signaling, it is not re

57 rotease by the viral TFP suggests a role for TFP in regulating protease function during HIV-1 replica

58 This metal-free TFP strategy will spur advancement in photonics and biop

59 ever, are unable to assemble into functional TFP despite their ability to localize to the membrane.

60 ower in a pilT mutant that lacked functional TFP.

61 However, TFP were not associated with the bacteria in the biofilm

62 ve determined the crystal structure of human TFP at 3.6- angstrom resolution.

63 approved drug trifluoperazine hydrochloride (TFP), which has been shown to inhibit FOXO1 nuclear expo

64 ation of the fluorescence of the immobilized TFP and the fluorogenic group provided a direct tool to

65 ts a novel genotype-phenotype correlation in TFP deficiency; that is, mutations in exon 9 of the alph

66 ry light chain (RLC) phosphorylation only in TFP-treated, but not in untreated, permeabilized smooth

67 ible to understand the interaction of intact TFP with HIV-1 protease under conditions of crystal grow

68 This predicted physical mechanism involving TFP was confirmed in vitro using pairwise mixtures of st

69 of wild-type M. catarrhalis and an isogenic TFP mutant to colonize the nasopharynx of the chinchilla

70 Here, we fluorescently label TFP in the pathogen Pseudomonas aeruginosa and track ful

71 bacterial species (>13,000 proteins) lacking TFP biosynthetic genes.

72 henyl)iodonium hexafluorophosphate (0.05 M), TFP (0.1 M), and PAIBN (0.02 M) was illuminated with a 6

73 By measuring TFP dynamics, we found that the retraction motor PilT wa

74 a TRIzol-based frozen precipitation method (TFP method), which shows rigor and reproducibility in hi

75 MPS+TFP more effectively protected renal function against re

76 ned to a perfusate group (G): G1=MPS, G2=MPS+TFP, G3=MPS+AL, and G4=MPS+TFP+AL.

77 ): G1=MPS, G2=MPS+TFP, G3=MPS+AL, and G4=MPS+TFP+AL.

78 hat the former is due to pulling by multiple TFP, whereas the latter is due to release by single TFP.

79 er of species were found to contain multiple TFP operons (pil, com and/or tad).

80 ey lacked EPS; however, they retained normal TFP and LPS.

81 Transframe octapeptide (TFP) Phe-Leu-Arg-Glu-Asp-Leu-Ala-Phe, the N terminus of

82 Assays examining the ability of TFP to block S100A4-mediated disassembly of myosin-IIA f

83 ginosa coordinates the motorized activity of TFP to power efficient surface motility.

84 s local concentration changes at the base of TFP during extension and retraction that change with sub

85 scaffold enabling spatial colocalization of TFP and Chp system components to coordinate signaling le

86 er, we identified specific configurations of TFP that lead to transitions between walking and crawlin

87 The LC17-induced contractions of TFP-treated muscles were abolished by prior addition of

88 l study by investigating the contribution of TFP to the early stages of M. catarrhalis colonization.

89 The successive control of TFP extension, attachment, retraction and detachment sug

90 through modulation of cAMP while control of TFP-dependent twitching motility is cAMP-independent.

91 Inherited deficiency of TFP is a recessive genetic disease, manifesting in hypok

92 example is the width of the distribution of TFP on the bacteria body.

93 These results suggest that the expression of TFP contributes to mucosal airway colonization.

94 itative measurements of multiple features of TFP dynamics.

95 In conclusion, the interaction of TFP and Low density seeding enhances construct material

96 olished motility and surface localization of TFP.

97 Finally, measurements of TFP dwell times on surfaces show that tension reinforced

98 Moreover, a surprisingly small number of TFP are needed to recapitulate movement signatures assoc

99 ormed by recent experimental observations of TFP.

100 ular self-sorting and selective packaging of TFP cargo into bacterial encapsulins during in vivo asse

101 e measurements reveal that the retraction of TFP is capable of deforming even stiff substrates.

102 e we discern a passive intercellular role of TFP during flagellar-mediated swarming of P. aeruginosa

103 The potential role of TFP in colonization by M. catarrhalis was further invest

104 oldest Eubacterial class and the ubiquity of TFP in this class suggests that a Clostridia-like ancest

105 including the T2SS, the T3SS, flagellum- or TFP-dependent motility, virulence in a mouse model of ac

106 ect on virus replication, mutation of the p1-TFP cleavage site led to noninfectious virus and the los

107 These findings implicate C. perfringens TFP in the ability of C. perfringens to adhere to and mo

108 as in tris(2,2,2-trifluoroethyl) phosphite (TFP), allows polymerization to proceed with a minimal am

109 , this metal-free triplet fusion photolysis (TFP) strategy can be further advanced by incorporating a

110 t two cell surface structures: type IV pili (TFP) and extracellular polysaccharides (EPS).

111 t MHQ inhibits the activity of Type IV Pili (TFP) and that TFP targeting can explain its dispersal ac

112 pendages known as flagella and type IV pili (TFP) are known to confer such motility.

113 Bacterial flagella and type IV pili (TFP) are surface appendages that enable motility and mec

114 s for surface exploration uses type-IV pili (TFP) as linear actuators to enable directional crawling.

115 ously shown that expression of type IV pili (TFP) contributes to mucosal colonization.

116 Type IV pili (TFP) function as mechanosensors to trigger acute virulen

117 Type IV pili (TFP) function through cycles of extension and retraction

118 In Gram-negative bacteria, type IV pili (TFP) have long been known to play important roles in suc

119 he extension and retraction of type IV pili (TFP) on solid surfaces, which requires both TFP and exop

120 lity to walk on surfaces using type IV pili (TFP), a motility mechanism known as twitching(1,2).

121 ) and their associated toxins, type IV pili (TFP), and flagella.

122 face association of individual Type IV Pili (TFP), and is informed by recent experimental observation

123 e III secretion system (T3SS), type IV pili (TFP), and multiple secreted toxins and degradative enzym

124 lity has been shown to require type IV pili (TFP), exopolysaccharide (EPS; a component of fibrils) an

125 Type IV pili (TFP), filamentous surface appendages primarily comprised

126 ility mechanism is mediated by type IV pili (TFP), linear actuator appendages that propel the bacteri

127 oss surfaces by using multiple Type IV Pili (TFP), motorized appendages capable of force generation v

128 , attachment and retraction of type IV pili (TFP), which pull the bacterium towards the site of attac

129 the production and function of type IV pili (TFP).

130 asures surface stiffness using type IV pili (TFP).

131 s lacks flagella but possesses type IV pili (TFP).

132 ells to QS is affected by the type IV pilus (TFP) retraction motors and the minor pilins.

133 some gram-negative bacteria, type IV pilus (TFP)-dependent gliding motility was necessary for effici

134 ce of agar plates by a unique type IV pilus (TFP)-mediated social motility that had not been previous

135 ts that a Clostridia-like ancestor possessed TFP, which evolved into the forms seen in many Gram-nega

136 d frameshift site, and hence did not produce TFP, was noninfectious.

137 Here we show that C. perfringens produces TFP and moves with an unusual form of gliding motility i

138 entified gene products involved in producing TFP in each strain.

139 essments of green total factor productivity (TFP) are supported, and possible lock-ins of the global

140 specification of total factor productivity (TFP) suggests an overall decline in productivity of -3.5

141 bilization of a visible fluorescent protein (TFP) modified to display a single cysteine residue was d

142 Thiocyanate-forming protein (TFP) from field-penny cress, Thlaspi arvense (Brassicace

143 ely detected as an 8-kDa transframe protein (TFP) in virions.

144 e-bound mitochondrial trifunctional protein (TFP) catalyzes beta-oxidation of long chain fatty acyl-C

145 We found that the FAO trifunctional protein (TFP) interacts with the NADH-binding domain of complex I

146 Human mitochondrial trifunctional protein (TFP) is a heterooctamer of four alpha- and four beta-sub

147 Using teal fluorescent proteins (TFP) engineered with a specific native C-terminal dockin

148 Remarkably, TFP binding results in the assembly of five Ca(2+)-S100A

149 rming of P. aeruginosa that does not require TFP extension or retraction.

150 esults in the assembly of five Ca(2+)-S100A4/TFP dimers into a tightly packed pentameric ring.

151 rative formation of a similarly sized S100A4/TFP oligomer in solution.

152 Results show TFP (1 ng/mL TGF-beta1, 5 ng/mL bFGF, 10 ng/mL PDGF) sup

153 ereas the latter is due to release by single TFP.

154 nd depolymerization dynamics are stochastic; TFP are made at random times and extend, pause, and retr

155 sh a regulatory link between the Chp system, TFP and other cAMP-dependent virulence systems.

156 a the application of the tetrafluoropyridyl (TFP) phenol-protecting group.

157 A tetrafluorphenyl (TFP) ester of trimethyl psoralen (trioxalen) was synthes

158 the activity of Type IV Pili (TFP) and that TFP targeting can explain its dispersal activity.

159 ld be distributed all over the cell and that TFP-TFP interactions between cells should be a dominant

160 h the crystal structure and demonstrate that TFP binds to the target binding cleft of S100A4 in solut

161 Here, we report that TFP and some flagella share proteins PilO, PilN, and Pil

162 he unanticipated structural requirement that TFP motors need to have a minimal amount of effective an

163 We show that TFP-mediated crawling in Pseudomonas aeruginosa consiste

164 Our simulations showed that TFP of swarming P. aeruginosa should be distributed all

165 slow swarm expansion, we show in vitro that TFP help alter collective motion to avoid toxic compound

166 The TFP and AL combination should not be used together with

167 The TFP method showed lower cycle threshold (CT) values by 2

168 their levels in the recovered sncRNAs by the TFP method and by the commercial kit.

169 mercial kit, plasma sncRNAs extracted by the TFP method enriched more sncRNAs.

170 ubstitution of the acidic amino acids in the TFP by neutral amino acids and d or retro-d configuratio

171 most effective solution for HBD kidneys, the TFP additive (perfusate G2) more effectively reversed th

172 aggregated into microcolonies, mimicking the TFP-regulated surface response.

173 reactions and protection/deprotection of the TFP group, substitution patterns that do not conform to

174 formation including a catalytic role of the TFP involved.

175 l by manipulating the ATPase activity of the TFP motors to change TFP extension and retraction veloci

176 directly links the integral component of the TFP structural complex FimV, a peptidoglycan binding pro

177 Specifically, the TFP-PEO-3 film-based dielectric capacitors display simul

178 We predict that the TFP are broadly distributed over the bacteria pole in bo

179 f M. catarrhalis TFP and determined that the TFP expressed by this organism are highly conserved and

180 e necessary for S-motility: We show that the TFP of leading "locomotive" cells initiate the collectiv

181 cts between S100A4 dimers occurs through the TFP moieties.

182 is unclear how M. xanthus manages to use the TFP-EPS technology common to many bacteria to achieve it

183 Like their TFP homologs, flagellar PilO/PilN heterodimers formed a

184 Thus, TFP physically affect P. aeruginosa swarming by actively

185 al structure of human Ca(2+)-S100A4 bound to TFP.

186 ll-studied CaM antagonists: trifluoperazine (TFP) and W-13.

187 molecule inhibitors such as trifluoperazine (TFP).

188 ique biosensor-based assay, trifluoperazine (TFP) was identified as an inhibitor that disrupts the S1

189 (saracatinib), calmodulin [trifluoperazine (TFP)], or proviral integration site of Moloney murine le

190 ype, and we have identified trifluoperazine (TFP) as a compound that interferes with this phenotype c

191 Importantly, trifluoperazine (TFP), a Nupr1 inhibitor, alleviated UUO-induced renal fi

192 if allopurinol (AL) and/or trifluoperazine (TFP) added to the Belzer machine preservation solution (

193 depleted Ca(2+) stores, or trifluoperazine (TFP), a blocker of CSQ folding and aggregation, enhanced

194 smooth muscle treated with trifluoperazine (TFP).

195 zene (TFB) or 1,3,5-triformylphloroglucinol (TFP), our approach enables the efficient formation of a

196 mylbenzene (TFB) or triformylphloroglucinol (TFP).

197 Animals coexpressing the TRIM5(TFP) and TRIM5(CypA) alleles took significantly longer t

198 Mutant viruses with a C-terminally truncated TFP (19, 32, or 50 residues) had essentially a wild-type

199 Two TFP molecules bind within the hydrophobic target binding

200 al bacteria attached end to end and that two TFP-associated proteins, PilT and PilC, are needed for t

201 nt signatures associated with twitching: Two TFP can already produce movements reminiscent of recentl

202 Thus, the quick and unbiased TFP method enriches plasma sncRNA ranging from 30 to 60

203 nhibition of the HIV-1 protease by the viral TFP suggests a role for TFP in regulating protease funct

204 To do this, we dynamically visualized TFP extension, attachment and retraction events at high

205 ilT motors very rapidly and almost only when TFP encounter the surface, suggesting contact sensing.

206 crease in density (</=-0.03 g/cm(3)) whereas TFP induced an increase in both thickness (</=0.05 nm) a

207 While TFP slow swarm expansion, we show in vitro that TFP help

208 ith CHO (k = 2 x 10(6) M(-1) s(-1)) and with TFP (k = 2 x 10(8) M(-1) s(-1)).

209 pecies, regardless of their association with TFP biosynthetic operons.

210 e cells without TFP separate from cells with TFP.

211 osphorylation events; and when combined with TFP, it even more effectively reduces proliferation and

212 y entropy and appears to be competitive with TFP and W-13, which is consistent with occupation of sim

213 CoA dehydrogenase physically interacted with TFP, thereby creating a multifunctional energy protein c

214 That is, with TFP, Low density increases type II collagen content by o

215 To examine the interaction of S100A4 with TFP, we determined the 2.3 A crystal structure of human

216 Continuous treatment with TFP and a single dose of radiation reduced the number of

217 1, a lysosome marker, whereas treatment with TFP, an inducer of autophagy, resulted in decreased Col-

218 airwise mixtures of strains with and without TFP where cells without TFP separate from cells with TFP

219 ins with and without TFP where cells without TFP separate from cells with TFP.

220 um containing 1% methylcellulose, M. xanthus TFP-driven motility was induced in isolated cells and in