ライフサイエンスコーパス: TPH

1 TPH cells appear to be uniquely poised to promote B-cell

2 TPH is protected from dopamine-induced inactivation by r

3 TPH message levels doubled between early day and early n

4 TPH mRNA was elevated during pregnancy and lactation, an

5 TPH was induced by PRL in mammosphere cultures and by mi

6 TPH-IR (microCi/g) was higher in suicides than controls

7 TPH-IR, an index of the amount of TPH enzyme, in the DRN

8 in, synthesized by tryptophan hydroxylase-1 (TPH(1)), has been shown to play a key role in several ph

9 Overexpression of tryptophan hydroxylase-1 (TPH-1), an enzyme involved in 5-MTP synthesis, reduces r

10 We found that 1) TPH and SERT immunoreactivity in neurons of raphe magnus

11 followed by a gradual decline until P21; 3) TPH labeling in neurons of the ventrolateral medullary s

12 except for a significant fall at P12; and 4) TPH and SERT immunoreactivity in a number of other nucle

13 Redox cycling of a TPH-quinoprotein could also participate in the serotonin

14 on, H2O2, or tyrosinase alone does not alter TPH activity.

15 carbons (TPH) were 18 +/- 0.6 g/kg soil, and TPH carbon constituted approximately 40% of the dichloro

16 Apo-TPH exposed to NO cannot be reactivated by iron.

17 been proposed for specific polymorphisms at TPH (suicide-related behaviors and impulsivity), DRD3 (s

18 Because TPH mRNA are regulated by the endogenous pineal circadia

19 ranscriptomics highlight differences between TPH cells and T follicular helper cells, including alter

20 +P) using in situ hybridization and a 249 bp TPH cRNA probe generated with RT-PCR (n = 5 animals/grou

21 We have expressed brain TPH as a recombinant glutathione-S-transferase fusion pr

22 ins form a complex with phosphorylated brain TPH, thereby increasing its enzymatic activity and inhib

23 PD-1(hi)CXCR5(+) T follicular helper cells, TPH cells induce plasma cell differentiation in vitro th

24 d the percentage of PR-ir cells colocalizing TPH-ir in both raphe nuclei, regardless of sex and genot

25 lted in lower, more statistically comparable TPH values.

26 o (112 +/- 22) of a non-releasing composite (TPH) (p > 0.1).

27 n of nuclear ER-ir or PR-ir with cytoplasmic TPH-ir or GFAP-ir was observed in either sex or treatmen

28 DRN TPH-IR was higher in male suicide victims (MS) compared

29 l requires the serotonin biosynthetic enzyme TPH-1 in neurons and the serotonin receptor SER-7 in the

30 e presence of 5-hydroxytryptophan, epidermal TPH activity is completely absent.

31 ence of RANKL, osteoclast precursors express TPH(1) and synthesize serotonin.

32 We previously reported that, in Finns, TPH genotype was associated with suicidality, a pathophy

33 -dependent reduction in 5-HIAA, a marker for TPH inhibition, from baseline until week 4.

34 trahydrobiopterin (BH(4)) and tryptophan for TPH NDelta15 were not observed.

35 Cultures from TPH(1)(-/-) in the presence of macrophage colony-stimula

36 Removal of iron from TPH produces an apoenzyme with low activity that can be

37 ften close to a 10% of thrombin peak height (TPH) yet it can be larger or smaller depending on whethe

38 particularly mapped to peripheral T helper (TPH) cells displaying signs of sustained proliferation,

39 llotropes, phagraphene and tetra-penta-hepta(TPH)-graphene, have been obtained by dehydrogenative C-C

40 ending on whether the sample has low or high TPH value.

41 In general, the samples with high TPH are associated with elevated TCT.

42 Treatment of holo-TPH (iron-loaded) with the disulfide 5,5'-dithio-bis (2-

43 zed to generate a full-length model of human TPH.

44 determining the total petroleum hydrocarbon (TPH) content of soils is an indispensable process step.

45 or improved the total petroleum hydrocarbon (TPH) degradation by ~70% than open circuit control react

46 redictor of the total petroleum hydrocarbon (TPH) removal for temperatures between 370 and 470 C and

47 sults show that total petroleum hydrocarbon (TPH) removal rate almost doubled in soils close to the a

48 Total petroleum hydrocarbons (TPH) as a lumped parameter can be easily and rapidly mea

49 atment reduced total petroleum hydrocarbons (TPH) to below regulatory standards (typically <1% by wei

50 Total petroleum hydrocarbons (TPH) were 18 +/- 0.6 g/kg soil, and TPH carbon constitut

51 ymes, 5'-end triphosphonucleotide hydrolase (TPH) instead of PPH activity.

52 substrate availability and TRP hydroxylase (TPH) enzymatic activity, leading to accumulation of exog

53 ther this depends on tryptophan hydroxylase (TPH) 1, the critical enzyme for peripheral 5-HT synthesi

54 , the mRNA levels of tryptophan hydroxylase (TPH) 1, TPH2 (both are involved in serotonin synthesis),

55 Using tryptophan hydroxylase (TPH) 2 deficient (Tph2-deficient) mice that lack brain s

56 for 5-HT synthesis, tryptophan hydroxylase (TPH) and aromatic amino acid decarboxylase (AADC) are ex

57 -synthesizing enzyme tryptophan hydroxylase (TPH) and serotonin transporter (SERT) with semiquantitat

58 biosynthetic enzyme tryptophan hydroxylase (TPH) are poorly understood.

59 Tryptophan hydroxylase (TPH) as the rate-limiting enzyme of serotonin synthesis

60 Rabbit brain tryptophan hydroxylase (TPH) has been expressed in insect cells (Spodoptera frug

61 class of peripheral tryptophan hydroxylase (TPH) inhibitors is described.

62 otonin biosynthesis, tryptophan hydroxylase (TPH) is a potential target for this autoregulation.

63 Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in the bios

64 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in serotonin biosynthes

65 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of 5-H

66 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of ser

67 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of the

68 pression of mRNA for tryptophan hydroxylase (TPH) was examined in ovariectomized (spayed) control, E-

69 biosynthetic enzyme tryptophan hydroxylase (TPH) was expressed in nearly 10% of neurons following tr

70 l Trp metabolism via tryptophan hydroxylase (TPH) with its downstream cascade, including serotonin an

71 hydroxylase (TH) and tryptophan hydroxylase (TPH), and draw an evolutionary scenario for this cell po

72 sis of CART and CART+tryptophan hydroxylase (TPH), CART+estrogen receptors (ER) or CART+progesterone

73 t in neuropsychiatry-tryptophan hydroxylase (TPH), dopamine transporter protein (SLC6A3), D3 dopamine

74 ymatic activities of tryptophan hydroxylase (TPH), indoleamine 2,3-dioxygenase (IDO), and monoamine o

75 synthesizing enzyme, tryptophan hydroxylase (TPH), or to the astrocytic marker, glial fibrillary acid

76 at the mRNA encoding tryptophan hydroxylase (TPH), the first enzyme in the melatonin synthetic pathwa

77 Exposure of tryptophan hydroxylase (TPH), the initial and rate-limiting enzyme in the biosyn

78 Tryptophan hydroxylase (TPH), the initial and rate-limiting enzyme in the biosyn

79 gic markers, such as tryptophan hydroxylase (TPH)- or 5-HT-positive cells and TPH2 RNA levels, were u

80 ing in the number of tryptophan hydroxylase (TPH)-positive neurons in the DR of wild-type (WT) mice.

81 xpressing VGluT3 and tryptophan hydroxylase (TPH).

82 olecule inhibitor of tryptophan hydroxylase (TPH).

83 synthesizing enzyme, tryptophan hydroxylase (TPH).

84 t -7/-10 kb in human tryptophan hydroxylase (TPH)2 were probed.

85 otonin biosynthesis (tryptophan hydroxylase [TPH] and aromatic amine decarboxylase).

86 etermine the amount of TPH immunoreactivity (TPH-IR) in the dorsal (DRN) and median (MRN) raphe nucle

87 arly day produced a modest increase (50%) in TPH message levels but had no effect at other times.

88 in the synthesis of 5-HT, and alterations in TPH could explain some of these findings.

89 ed sites, such as CCR2, CX3CR1, and CCR5, in TPH cells.

90 em nuclei exhibited a significant decline in TPH and SERT immunoreactivity during the critical period

91 oradiographs revealed a ninefold increase in TPH mRNA in E-treated macaques compared to spayed animal

92 mal-looking neurons and a marked increase in TPH-positive spindle-shaped cells.

93 ones covalently modify cysteinyl residues in TPH, leading directly to the loss of catalytic activity,

94 ence that the decrease in bone resorption in TPH(1)(-/-) mice is cell-autonomous.

95 Bone resorption in TPH(1)(-/-) mice, as assessed by biochemical markers and

96 Single-nucleotide polymorphisms (SNPs) in TPH genes have been already described in associations wi

97 neurotoxic to 5-HT neurons, that inactivate TPH in vivo and are now known to produce NO and other re

98 detection (HPLC-EC) analysis of inactivated TPH revealed the formation of cysteinyl-dopamine residue

99 These data indicate that NO inactivates TPH by selective action on critical SH groups (i.e., cys

100 ort of O3 to TPH bound to soil and increased TPH removal.

101 These data indicate that E induces TPH gene expression in nonhuman primates and that the ad

102 t the benchmark dose of 3.4 kg O3/kg initial TPH, TPH carbon was reduced by nearly 6 gC/kg soil (40%)

103 eurons and CART fibers appeared to innervate TPH-positive serotonin neurons in the dorsal raphe.

104 oautoradiography, using an antibody to label TPH, was performed, slides exposed to film and autoradio

105 portable FT-NIR spectrometers to predict low TPH levels in various soil types through both-soil-speci

106 autoreceptor agonist, CGS 12066A, can lower TPH mRNA levels and promoter activity.

107 detail bone remodeling in growing and mature TPH(1) knockout mice (TPH(1)(-/-)).

108 in growing and mature TPH(1) knockout mice (TPH(1)(-/-)).

109 Exposure of dopamine-modified TPH to redox-cycling staining after SDS-PAGE confirmed t

110 It enhanced serotonin levels and modulated TPH, IDO, and MAO activities in a tissue-specific manner

111 More TPH may be an upregulatory homeostatic response to impai

112 Moreover, TPH was enzymatically active (112.8+/-36 pmol/mg per h)

113 The inactivation of native TPH by NO cannot be reversed by either iron or DTT.

114 For example, elevated TCT (above 15% of TPH) was associated with either very low or very high TP

115 TPH-IR, an index of the amount of TPH enzyme, in the DRN is higher in depressed suicides.

116 We sought to determine the amount of TPH immunoreactivity (TPH-IR) in the dorsal (DRN) and me

117 The analysis of TPH-IR area and density at each DRN rostrocaudal levels

118 ther dissect the regulation and catalysis of TPH.

119 Recently, we developed a class of TPH inhibitors based on xanthine-benzimidazoles, charact

120 This class of TPH inhibitors exhibits excellent potency in in vitro bi

121 the method and improve the comparability of TPH data, crucial GC-based quantification issues were ex

122 e control group, the lowest concentration of TPH was associated with higher serotonin levels (TT: Rs

123 The dephosphorylation of TPH by protein phosphatase-1 was inhibited by 14-3-3 pro

124 a quick-preferably on-site-determination of TPH content is valuable.

125 for rapid and quantitative determination of TPH content of soils from two different sites by diffuse

126 LA that continuously fueled the expansion of TPH cells expressing a pathogenic cytokine effector prog

127 es a concentration-dependent inactivation of TPH as well.

128 nzoic acid) (DTNB) causes an inactivation of TPH that is readily reversed by dithiothreitol (DTT).

129 hat cause dopamine-dependent inactivation of TPH.

130 ce is consistent with a greater induction of TPH 2, and 5-HTT by EB in DRN that play key roles in emo

131 The pharmacological inhibition of TPH activity blocked these effects.

132 Local inhibition of TPH in the gastrointestinal tract might reduce mucosal p

133 The inherent instability of TPH has prevented a crystallographic structure from bein

134 ies that distinguish between the isoforms of TPH will allow studies of the differential regulation of

135 are effective in preventing the labeling of TPH by [3H]dopamine.

136 ion is probably not mediated at the level of TPH gene transcription.

137 forskolin+TPA) yielded the greatest level of TPH induction (15.6%).

138 while for the AP group the highest levels of TPH among the TT genotype were associated with lower lev

139 e significantly higher single-cell levels of TPH mRNA in serotonergic neurons of the dorsal raphe in

140 001, and in the GG genotype higher levels of TPH were associated with higher levels of serotonin (GG:

141 Heretofore, probes used for localization of TPH protein or mRNA could not distinguish between the TP

142 here was a marked reduction in the number of TPH-positive normal-looking neurons and a marked increas

143 We have found that phosphorylation of TPH by cAMP-dependent protein kinase increased the activ

144 ns play important roles in the regulation of TPH activity.

145 Regulation of TPH via microphthalmia-associated transcription factor a

146 hat Tyr235 (Y235), within the active site of TPH, appears to be involved as a tryptophan substrate or

147 the regulation and substrate specificity of TPH, as well as providing a basis to understand as yet t

148 addition of P has little additive effect on TPH gene expression.

149 roaches did not have a significant effect on TPH results.

150 d the hydroxylase activity of phosphorylated TPH by approximately 45%.

151 that 14-3-3 proteins bind to phosphorylated TPH with an affinity constant (Ka) of 4.5 x 10(7) M-1.

152 14-3-3 proteins interact with phosphorylated TPH.

153 (RMSEC/RMSECV) of 1043/1106 and 741/785 ppm TPH, respectively, and the average absolute prediction e

154 Once thought to be a single-gene product, TPH is now known to exist in two isoforms-TPH1 is found

155 educing agents and antioxidants that protect TPH from inactivation are effective in preventing the la

156 Highly purified TPH catalytic core, like the native enzyme from brain, i

157 statistical analyses show that the residual TPH has a strongly positive correlation with hydrocarbon

158 terval, resulting in the order CEN-LDHA-SAA1-TPH-D11S1310-(D11S1888/KCNC1 )-MYOD1-D11S902D11S921-D11S

159 the amino terminal autoregulatory sequence (TPH NDelta15).

160 pulation of DR-MOR neurons expressing solely TPH, which were not activated in hyperalgesia during spo

161 n of PD-1(hi)CXCR5(-) 'peripheral helper' T (TPH) cells that express factors enabling B-cell help, in

162 ss of catalytic activity, and establish that TPH could be a target for dopamine-quinones in vivo afte

163 Together, our results suggest that TPH-1 may serve as a target in the treatment of CKD.

164 -specific, because MEKK did not activate the TPH promoter in nonneuronal cell lines.

165 in or mRNA could not distinguish between the TPH isoforms because of extensive homology shared by the

166 The intron 7 variant in the TPH gene showed significant evidence for linkage to suic

167 The association of the TPH 17 779C (L) allele to suicidality in impulsive offen

168 The status of the TPH A779C allele as a marker for suicidality was replica

169 nalyses of a polymorphism in intron 7 of the TPH gene with suicidality, alcoholism, and the Karolinsk

170 The amplitude of the TPH mRNA rhythm was increased to 4-fold by culturing the

171 repression of MAP kinase stimulation of the TPH promoter.

172 od PetroFLAG) were performed to quantify the TPH content in samples collected from a site contaminate

173 st this hypothesis, we first showed that the TPH promoter can be activated 20-fold by mitogen-activat

174 A functional variant(s) in or close to the TPH gene may predispose individuals to suicidality and o

175 monly used parameters such as clotting time, TPH or Thrombin Production Rate (TPR).

176 in the soil improved mass transport of O3 to TPH bound to soil and increased TPH removal.

177 benchmark dose of 3.4 kg O3/kg initial TPH, TPH carbon was reduced by nearly 6 gC/kg soil (40%), whi

178 DTNB-treated TPH [sulfhydryl (SH)-protected] exposed to NO is returne

179 With supplemental P treatment, TPH mRNA signal was increased fivefold over spayed anima

180 uced specific activity compared to wild-type TPH ( approximately 5 % residual activity).

181 ignificantly increased compared to wild-type TPH (42 microM).

182 kinetic analyses were performed on wild-type TPH and a deletion construct that lacks the amino termin

183 ere not phosphorylated, and unphosphorylated TPH was not activated by 14-3-3 proteins.

184 ing renal inflammation and fibrosis, whereas TPH-1 deficiency exacerbates renal injury and fibrosis b

185 PR-ir or ER alpha-ir often colocalized with TPH-ir.

186 rom soil samples of two different sites with TPH reference values ranging from 350 to 30,000 ppm, as