ライフサイエンスコーパス: TREC

1 TREC levels correlated with the frequency of phenotypica

2 TREC levels rose weeks after HSCT and could be detected

3 TREC NBS in California has achieved early diagnosis of S

4 TREC numbers can be measured in DNA isolated from the dr

5 TREC test specificity was excellent: only 0.08% of infan

6 TREC testing of newborns is now being performed in sever

7 TRECs peaked earlier and with higher values (P <.01) in

8 TRECs were present in all patients in both total lymphoc

9 ance judgments produced in the 2004 and 2005 TREC Genomics Track and observe significant correlations

10 In patient 8 TREC levels were borderline and KREC levels were abnorma

11 and KREC levels were abnormal; in patient 9 TRECs were undetectable, whereas KREC levels were normal

12 ble TRECs and might therefore be missed by a TREC-based newborn screening program.

13 vels and will therefore not be detected in a TREC-based newborn screening program.

14 nt superimposed onto the trapping rings of a TREC.

15 data from 3,030,083 newborns screened with a TREC test.

16 37 weeks' gestation had at least 1 abnormal TREC assay (TREC values < 25/microL), 11 of whom had sam

17 Infants with low or absent TRECs can thus be identified and referred for confirmato

18 Additionally, TRECs were detected in genomic DNA obtained from Guthrie

19 l division have the same potential to affect TREC concentration at any age in healthy people.

20 However, not all TREC decreases could be attributed to increased T-cell p

21 mance rankings generated by our approach and TREC.

22 over of memory CD4(+) and CD8(+) T-cells and TREC depletion in naive CD4(+) T-cells, although naive T

23 w cytometry), in vitro responses to PHA, and TREC levels, all measured at presentation, were compiled

24 Naive CD4(+) lymphocyte phenotype and TREC levels were not significantly different in patients

25 d with published data on Ki67 expression and TRECs within naive CD4+ T cells in healthy individuals.

26 The TREC test has clinical validity and TRECs, as predicted, are an excellent biomarker of poor

27 4 times that of the state-of-the-art aqueous TREC electrolyte potassium ferricyanide.

28 Recent research initiatives such as TREC Genomics and BioCreAtIvE strongly point to the meri

29 estation had at least 1 abnormal TREC assay (TREC values < 25/microL), 11 of whom had samples analyze

30 Because TRECs are a general surrogate marker for severe T lympho

31 Thymic function failure (sj/beta-TREC ratio <10) was independently associated with HIV pr

32 cell rearrangement excision circles (sj/beta-TREC ratio) overcomes this limitation but has only been

33 ance of thymic function, measured by sj/beta-TREC ratio, in HIV disease progression by analyzing a la

34 of thymic function, measured by the sj/beta-TREC ratio, on CD4 T-cell maintenance in prospective HIV

35 n peripheral blood samples using the sj/beta-TREC ratio.

36 ths after bone marrow transplantation (BMT), TREC levels remained low for 3 months after BMT.

37 t ADA deficiency had normal TREC levels, but TRECs were undetectable in blood samples taken from the

38 that thymectomy decreased CD4 or CD8 T cell TREC concentrations most when thymopoiesis was active be

39 no significant mean difference in PB T cell TREC levels between ages 40 and 80 years.

40 hymectomized MG patients had lower PB T cell TREC levels than did age-matched normal subjects (p < 0.

41 ell called the trapping ring electrode cell (TREC) has been conceived, simulated, developed, and test

42 applied to the Trapping Ring Electrode Cell (TREC).

43 ometry) and T-cell receptor excision circle (TREC) abundance.

44 notypic and T cell receptor excision circle (TREC) analysis confirmed a recent thymic origin of the e

45 Using T-cell receptor excision circle (TREC) analysis, we directly measured in vivo replicative

46 ening using T-cell receptor excision circle (TREC) analysis.

47 T-cell receptor excision circle (TREC) and kappa-deleting recombination excision circle (

48 D using the T-cell receptor excision circle (TREC) assay began in Wisconsin in 2008; 5 infants with S

49 T-cell receptor excision circle (TREC) assays for the evaluation of thymus activity revea

50 elopment of T-cell receptor excision circle (TREC) assays of thymopoiesis, cytokine-flow cytometry as

51 TCR excision circle (TREC) content has been used as a proxy for thymic export

52 cell receptor rearrangement excision circle (TREC) levels of 2000 or more copies/mug DNA.

53 typing, and T cell receptor excision circle (TREC) levels were measured.

54 signal joint (sj) TCR delta excision circle (TREC) levels, a molecular marker for active thymopoiesis

55 signal from T cell receptor excision circle (TREC) loss during V(D)J recombination of the T cell rece

56 ession, and T cell receptor excision circle (TREC) measurements in T cells were evaluated at weeks 0

57 , including T-cell receptor excision circle (TREC) quantification, in patients with MHC-II deficiency

58 7 staining, T-cell receptor excision circle (TREC) quantitation in sorted CD4 and CD8 cells, and thym

59 results of T-cell receptor excision circle (TREC) testing newborns in pilot states indicate that thi

60 e counts of T cell receptor excision circle (TREC)-containing CD4 T cells (presumed recent thymic emi

61 numbers of T-cell receptor excision circle (TREC)-positive T cells, indicating increased thymopoiesi

62 evels of TCR rearrangement excision circles (TREC) and parameters of cell proliferation, including Ki

63 TCR excision circles (TREC) as a marker of thymic output exponentially decreas

64 heir lower T-cell receptor excision circles (TREC) content and shorter telomeres proved they had divi

65 T cell receptor excision circles (TREC) have been recently used to assess thymic output du

66 Assays for T cell receptor excision circles (TREC) have been utilized in human, primate, and mouse mo

67 T cell repertoire, low TCR excision circles (TREC) values, and a predominance of CD45RO(+) T cells.

68 Levels of T receptor excision circles (TREC) were comparable in purified CD4(+)CD25(+) and CD4(

69 ell receptor rearrangement excision circles (TREC) were substantially reduced in RA patients; TREC le

70 ell receptor rearrangement excision circles (TREC) within peripheral T cell populations provides insi

71 h level of T-cell receptor excision circles (TREC), demonstrating de novo T lymphopoiesis, and functi

72 of macaque T-cell receptor excision circles (TREC).

73 quantified T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles

74 itation of T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles

75 levels of T cell receptor excision circles (TRECs) and T cell lymphopenia at birth, who carried hete

76 levels of T-cell receptor excision circles (TRECs) and the lowest frequencies of Ki67(+) T cells, wh

77 eceptor gene rearrangement excision circles (TRECs) as a measure of recent thymic emigrant cells in p

78 to detect T-cell receptor excision circles (TRECs) began in Wisconsin in 2008, and SCID was added to

79 ell receptor rearrangement excision circles (TRECs) declined from 47,946 to 26,510 copies per 10(6) n

80 ell-receptor rearrangement excision circles (TRECs) decreased.

81 ceptor (TCR) rearrangement excision circles (TRECs) have been used as markers for recent thymic emigr

82 Assay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth permits po

83 ell receptor rearrangement excision circles (TRECs) in human thymus.

84 ell receptor rearrangement excision circles (TRECs) in measles patients were increased in CD8+ T cell

85 urement of T-cell receptor excision circles (TRECs) in peripheral blood is a means of quantifying rec

86 ication of T-cell receptor excision circles (TRECs) or tandem mass spectrometry (tandem-MS) analysis

87 ilution of T cell receptor excision circles (TRECs) or the dye CFSE, and clonal expansion has been do

88 rations of T-cell-receptor excision circles (TRECs) were 10 per 100 T cells.

89 function, T-cell receptor excision circles (TRECs) were examined in CD4(+) and CD8(+) cells from a c

90 nd T-cell antigen receptor excision circles (TRECs) were measured before transplantation and sequenti

91 cell-receptor signal joint excision circles (TRECs) were not detected in reconstituting T cells in do

92 esting for T-cell receptor excision circles (TRECs), a DNA biomarker of normal T-cell development, ha

93 Finally, no TRA/delta excision circles (TRECs), a marker of TRA/delta locus rearrangements, were

94 ing signal joint TCR delta excision circles (TRECs), a molecular marker of thymus emigrants that have

95 ertoire, TCR rearrangement excision circles (TRECs), and hematopoietic chimerism were studied in the

96 T-cell receptor excision circles (TRECs), markers of T cells generated de novo, were quant

97 tive TCRalpha joins on TCR excision circles (TRECs), which are the products of secondary V/J recombin

98 ell receptor rearrangement excision circles (TRECs).

99 tection of T-cell receptor excision circles (TRECs).

100 urement of T cell receptor excision circles, TRECs), and studies of thymus biopsies in untreated and

101 tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome

102 ified therapeutic receptor-effector complex (TREC) suitable for gene therapy.

103 in the Text Retrieval Evaluation Conference (TREC), organized annually for the past 15 years, to supp

104 hermally regenerative electrochemical cycle (TREC) is an attractive approach which uses the temperatu

105 ermally regenerative electrochemical cycles (TRECs), which utilize reversible electrochemical process

106 mized mice resulted in patterns of decreased TREC frequency and increased total TREC number similar t

107 rd blood grafts had no evidence of decreased TREC induced by immunosuppressive prophylaxis drugs.

108 ients with MHC-II deficiency have detectable TRECs and might therefore be missed by a TREC-based newb

109 They also have significantly different TREC content indicating different division histories and

110 At discharge, TRECs in CD4+ T cells (P = 0.05) and circulating levels

111 uggested that IL-7 can also directly enhance TREC generation.

112 ield-collected samples from the pan-European TREC expedition.

113 Here we develop T cell exome TREC tool (T cell ExTRECT), a method for estimation of T

114 mpared to CD4(+), CD4(-) iNKTs contain fewer TRECs, express higher levels of IL-2Rbeta, and prolifera

115 At baseline, patients exhibited fewer TRECs than did uninfected control subjects.

116 evaluation of thymus activity revealed fewer TRECs in both transplant groups compared with healthy do

117 The median number of copies was 120 for TRECs (IQR = 92-168) and 136 (IQR = 91-183) for CD patie

118 In this study, a quantitative assay for TRECs was used to measure T-cell neogenesis in adult pat

119 peat DNA isolation with quantitative PCR for TRECs and a genomic control, and immunophenotyping was p

120 Here, we demonstrate a charging-free TREC consisting of an inexpensive soluble Fe(CN)6(3-/4-)

121 This charging-free TREC system may have potential application for harvestin

122 arily evaluated using the Genomics data from TREC (Text Retrieval Conference) 2006 Genomics Track.

123 were conducted on four medical datasets from TREC, using between 62 and 125 candidate retrieval syste

124 Furthermore, TRECs correlated positively with the number of CD4 and n

125 r the absolute quantification of rare genes, TRECs in our case, by droplet digital PCR (ddPCR), from

126 s isolated from peripheral blood had greater TREC quantities than their CD4(+)CD25(+) counterparts, s

127 Finally, 51 patients had TREC levels measured.

128 ell output by the thymus as revealed by high TREC values and a polyclonal T cell repertoire demonstra

129 ipients of matched sibling grafts had higher TREC levels.

130 ey have nationally or regionally implemented TREC-based NBS.

131 n the number of RTE generated per day and in TREC concentration during an 80-year lifespan.

132 s well as common practices and challenges in TREC-based NBS.

133 t is the major contributor to the decline in TREC concentration within CD4(+) T cells, whereas both i

134 ecreased thymic output induce the decline in TREC concentration within CD8(+) T cells.

135 rimarily induces an age-dependent decline in TREC concentrations within both CD4(+) and CD8(+) T cell

136 rus (HIV)-infected patients, this decline in TREC levels did at times correlate with an increased lev

137 rapid viral recrudescence and a decrease in TREC levels.

138 Decreases in TREC frequency were attributable to dilution secondary t

139 Following the evaluation process used in TREC 2017 LiveQA, we find that our approach exceeds the

140 c suppression) also had substantial gains in TRECs.

141 The increase in TRECs after antiretroviral therapy was greater in infant

142 mmune organs, accounting for the increase in TRECs in the total peripheral lymphoid pool; and (3) no

143 caques (n = 23) revealed stable or increased TREC levels at 20 to 34 weeks postinfection.

144 et of methods with data from two independent TREC Genomics Track evaluations.

145 T cell death also influences TREC concentration, but to a lesser extent.

146 The development of the porcine signal joint TREC assay should enable a more direct quantification of

147 hain reaction (PCR) to quantify signal-joint TRECs (sjTRECs) before and after transplantation.

148 Low TREC-containing CD4 T cell counts were associated with o

149 Omenn syndrome, and ZAP70 deficiency had low TREC levels.

150 Samples with initial low TREC numbers had repeat DNA isolation with quantitative

151 the most important factor that predicted low TREC levels even years after HSCT.

152 At week 0, NRs had lower TREC levels per 1x106 T cells and higher levels of T cel

153 ipheral effects of IL-7 on RTEs, we measured TREC content and peripheral naive T-cell subsets and tur

154 Most TREC systems still require external electricity for char

155 culating T cell numbers, particularly naive (TREC+) T cells.

156 with delayed-onset ADA deficiency had normal TREC levels, but TRECs were undetectable in blood sample

157 R222C mutation in the IL2RG gene, had normal TREC levels.

158 nts with conditions including TD have normal TREC levels and will therefore not be detected in a TREC

159 monstrate (1) that total TREC number and not TREC frequency accurately reflects quantitative changes

160 Tandem-MS but not TREC quantification identifies newborns with delayed- or

161 eoretical limit was achieved using the novel TREC technology; over 420,000 resolving power was observ

162 played thymic regeneration and attainment of TREC levels of 2000 or more copies/mug DNA (P = .005).

163 m), CD95(dim)) and its high concentration of TREC.

164 The counts of TREC(+) CD4 T cells in transplant recipients younger tha

165 In contrast, the counts of TREC(+) CD4 T cells were lower in transplant recipients

166 A decline of TREC levels and a decrease in the number of CD45RA(+) ce

167 enia." Even though complete harmonization of TREC-based NBS programs across Europe will remain challe

168 In summary, reduced levels of TREC can be observed beginning at 16 to 30 weeks post-SI

169 naive CD8(+) cells display higher levels of TREC than their CD103(-) naive counterparts, and these c

170 This suggests that the loss of TREC during HIV infection can arise from a combination o

171 feration did not increase, despite a loss of TREC within naive CD4+ T cells.

172 The loss of TREC-positive T cells could be a consequence of a primar

173 Simultaneous measurement of TREC and KREC copy numbers in Guthrie card samples readi

174 er relative change (n-fold) in the number of TREC+ T cells/mul than in naive T-cell counts was observ

175 ry of resolved GVHD had decreased numbers of TREC, compared with those with no GVHD.

176 Stability of TREC frequencies throughout the period of repertoire gen

177 We sought to report the first 2 years of TREC NBS in California.

178 on, whereas the other tracks the dynamics of TRECs.

179 ce, our analysis showed a lower frequency of TRECs with male-reactive V17J57 joins in male mice.

180 ction demonstrated that the highest level of TRECs (14 692 copies/10 000 cells) was present in the CD

181 Measurement of TRECs may serve as a useful tool for evaluating immune r

182 ll lymphopenia by quantitating the number of TRECs contained in a 3.2-mm punch (approximately 3 micro

183 as also associated with increased numbers of TRECs in CD3(+) T cells (P <.001) and with conversion to

184 hymic activity, which was estimated based on TREC levels and T-cell receptor (TCR) genes, as well as

185 only published results, a 37-item survey on TREC-based NBS was sent to representatives of 46 Europea

186 requency of in-frame, male-specific joins on TRECs in male and female HYbeta transgenic mice.

187 As a general paradigm, TRECs for enhancement of other G-protein signaling appea

188 ) were substantially reduced in RA patients; TREC levels in young adult patients matched those of con

189 poiesis resulted in a significant fall in PB TREC(+) T cells postthymectomy.

190 ted why previous attempts at cloning the pig TREC using known sjTREC sequences were unsuccessful.

191 of T-cell receptor excision circle-positive (TREC(+)) cells in the peripheral blood and dramatic incr

192 Unlike in the detection of primate TRECs, initially the use of similar primers close to the

193 deoxyadenosine and real-time PCR to quantify TREC levels.

194 evaluate whether tandem-MS and quantitative TREC analyses of DBSs could identify newborns who had de

195 Here we use the topographic and recognition (TREC) mode of an atomic force microscope to visualize UC

196 in some patients was associated with reduced TREC levels, but infusion of mature donor CD4(+) T cells

197 development of the porcine signal joint (sj) TREC assay, and provide a likely reason for previous dif

198 We further apply this model for studying TREC concentration during HIV-1 infection.

199 IV-infected macaques (n = 4) determined that TREC levels decreased between 24 and 48 weeks postinfect

200 his approach is complicated by the fact that TREC levels also are determined by turnover within the n

201 8(+) stage in native thymus, suggesting that TREC generation occurred following the cellular division

202 The TREC Genomics Track has recently been introduced to meas

203 The TREC permits the ability to maintain coherent ion motion

204 The TREC test has clinical validity and TRECs, as predicted,

205 Exactly 71,000 infants were screened by the TREC assay.

206 on efficiency of 2.0% can be reached for the TREC operating between 20 and 60 degrees C.

207 Furthermore, the TREC did not display tachyphylaxis to prolonged agonist

208 culture system, exogenous IL-7 increased the TREC frequency in fetal as well as infant thymus, indica

209 In a statewide screening program, use of the TREC assay performed on NBS cards was able to identify i

210 The performance of the TREC is compared to a closed cylindrical cell at differe

211 This review will provide an overview of the TREC screening assay and an update of the findings from

212 a number of reranking experiments using the TREC 2005 genomics track test collection in which scores

213 newborn population-based screening using the TREC assay, including the evaluation and care of infants

214 Routine screening of all newborns with the TREC test, implemented as part of an integrated public h

215 division remains relatively unchanged, then TREC concentration indeed reflects thymic output.

216 A high-throughput TREC quantitative PCR assay with DNA isolated from routi

217 decreased TREC frequency and increased total TREC number similar to those in IL-7-treated thymus-inta

218 ymus-intact mice resulted in increased total TREC numbers, consistent with RTE accumulation.

219 These results demonstrate (1) that total TREC number and not TREC frequency accurately reflects q

220 ships will help in the move toward universal TREC-based screening for all newborns, resulting in more

221 During phase 2 (after 16 to 30 weeks), TREC levels declined in both T-cell populations.

222 During phase 1 (16 to 30 weeks), TREC levels remained stable or increased within both the

223 tifies newborns with PNP deficiency, whereas TREC or KREC measurement alone can fail.

224 and FoxP3 expression on CD4(+) T cells, with TREC levels per 1x106 CD4(+) T cells decreasing signific

225 whether age alone inversely correlated with TREC levels.

226 ivalent of at least 37 weeks' gestation with TREC values of less than 25/microL.

227 ng bpref measured with NT Evaluation or with TREC evaluations.

228 positively correlates in these patients with TREC levels.

229 ll turnover should be examined together with TREC concentration as a measure of RTE.

230 After the age of 70 years, TRECs only slightly declined, but homeostatic proliferat