ライフサイエンスコーパス: TTF-1

1 TTF-1 activates transcription of target genes, including

2 TTF-1 binds to and activates the transcription of genes

3 TTF-1 directly interacts with the conserved DNA binding

4 TTF-1 dose-dependently activated alpha(7) transcription

5 TTF-1 interacted with SRC-1 and CBP in vitro.

6 TTF-1 is a critical regulator of transcription for the s

7 TTF-1 is acetylated by nuclear receptor coactivators inc

8 TTF-1 is amplified in lung cancers, suggesting that it i

9 TTF-1 is an enhancer of class I promoter activity; Pax-8

10 TTF-1 is an essential transcription factor required for

11 TTF-1 knockdown and overexpression studies showed that T

12 TTF-1 protein synergistically stimulated the hSP-B promo

13 TTF-1 transactivated SP-C-chloramphenicol acetyltransfer

14 TTF-1 was subsequently found in lung tissue, where it re

15 ification of thyroid transcription factor 1 (TTF-1 or NKX2-1) biochemical activity as a transcription

16 addition to thyroid transcription factor 1 (TTF-1) and hepatocyte nuclear factor 3alpha (HNF-3alpha)

17 Thyroid transcription factor 1 (TTF-1) is a 42 kDa homeodomain (HD) containing the tissu

18 Thyroid transcription factor 1 (TTF-1) is a homeodomain-containing nuclear transcription

19 hat PPFP and thyroid transcription factor 1 (TTF-1) physically interact, and that these transcription

20 s (RARs) and thyroid transcription factor 1 (TTF-1) stimulated SP-B gene expression in respiratory ep

21 d binding of thyroid transcription factor 1 (TTF-1) to an upstream response element (TTF-1-binding el

22 15, and that thyroid transcription factor 1 (TTF-1) transactivates promoter activity.

23 Thyroid transcription factor 1 (TTF-1) was identified for its critical role in thyroid-s

24 orylation of thyroid transcription factor 1 (TTF-1), expressed selectively in developing lung epithel

25 Thyroid transcription factor 1 (TTF-1), hepatocyte nuclear factor 3alpha (HNF-3alpha), a

26 tion factor, thyroid transcription factor 1 (TTF-1), in rat FRTL-5 thyrocytes.

27 dependent on thyroid transcription factor 1 (TTF-1).

28 RAS, NKX2-1 [thyroid transcription factor 1 (TTF-1)], STK11, SMARCA4, GNAS, and ALK rearrangements we

29 Thyroid transcription factor 1 (TTF-1/Nkx-2.1) plays a critical role in lung morphogenes

30 f endogenous thyroid transcription factor 1 (TTF-1/Nkx2.1) to the miR-29ab1 promoter in HFL type II c

31 I (NFI) and thyroid transcription factor 1 (TTF-1; also called Nkx2.1).

32 the role of thyroid transcription factor-1 (TTF-1) and hepatocyte nuclear factor 3 (HNF3) in the dow

33 d that three thyroid transcription factor-1 (TTF-1) binding elements (TBEs) located within a 255 base

34 old, whereas thyroid transcription factor-1 (TTF-1) increased the activity of these constructs 12-18-

35 Thyroid transcription factor-1 (TTF-1) is expressed in respiratory epithelial cells, whe

36 Thyroid transcription factor-1 (TTF-1), a member of the Nkx2 family of homeodomain-conta

37 rs including thyroid transcription factor-1 (TTF-1).

38 pressing rat thyroid transcription factor-1 (TTF-1).

39 -1 (TSEP-1); thyroid transcription factor-1 (TTF-1); and Pax-8.

40 ulator gene, Thyroid Transcription Factor-1 (TTF-1, also known as NKX2-1), is used as a marker by pat

41 Thyroid transcription factor-1 (TTF-1/Nkx-2.1) is required for formation of the lung and

42 A binding of thyroid transcription factor-1 (TTF-1/Nkx2.1), a master regulator of lung development.

43 s including thyroid transcription factors 1 (TTF-1; refs 4,5) and 2 (TTF-2; refs 6,7) and Pax8 are ab

44 TSEP-1, TTF-1, and/or Pax-8 are involved in TSH/cAMP-induced neg

45 Only Gli1 induces Nkx2.1/TTF-1(+) ventral forebrain neurons.

46 activity does not appear to be mediated by a TTF-1-imposed alteration on nucleotide excision repair.

47 at-beta, stimulated the phosphorylation of a TTF-1-flag fusion protein 6-7-fold in H441 pulmonary ade

48 Co-transfection with a TTF-1 expression vector moderately transactivated the -1

49 GATA-6, but not GATA-5, markedly activated TTF-1 gene transcription in HeLa cells.

50 In addition, TTF-1 interacted directly with RARalpha and TIF2 in the

51 Although TTF-1 is amplified in primary human lung cancers, it inh

52 ure, nuclear localization of CBP, SRC-1, and TTF-1 increased in ductular epithelium in association wi

53 taining demonstrated that both HNF-3beta and TTF-1 were detected in bronchiolar and alveolar type II

54 ts of cotransactivating with both GATA-6 and TTF-1 expression constructs were additive.

55 TF-1, supporting the concept that GATA-6 and TTF-1 might directly interact to influence target gene e

56 miR-29ab1 promoter in HFL type II cells, and TTF-1 increased miR-29ab1 promoter-driven luciferase act

57 ustered retinoic acid-responsive element and TTF-1 binding sites were identified in the enhancer regi

58 n which retinoic acid-responsive element and TTF-1 DNA binding sites overlap.

59 Protein-protein interactions between Erm and TTF-1 were demonstrated by mammalian two-hybrid assays a

60 REB, CREM, ATF-1, ATF-2 as well as c-Jun and TTF-1.

61 NFATc3 and TTF-1 activated the Sftpd promoter, synergized transcrip

62 roid-specific transcription factors Pax8 and TTF-1, leading to expression of the thyroid-specific tar

63 f H441 cells greatly stimulated both RAR and TTF-1 DNA binding to the hSP-B enhancer region in H441 c

64 RAR and TTF-1 synergistically stimulated the hSP-B promoter in H

65 In addition, RAR and TTF-1 were colocalized in mouse bronchiolar and alveolar

66 In the present studies, RAR and TTF-1 were colocalized in the nucleus of H441 cells.

67 protein-protein interactions between RAR and TTF-1 were demonstrated by the glutathione S-transferase

68 moter activity suggesting that Sp1, Sp3, and TTF-1 and HNF-3alpha interact cooperatively with SP-B pr

69 P-ribose) polymerases (PARP-2 and PARP-1) as TTF-1 interacting proteins that influence its transcript

70 sites, which were specifically identified as TTF-1 binding sites by gel retardation and antibody supe

71 factor NK2 homeobox 1 (NKX2-1; also known as TTF-1).

72 e findings identify miR-29 family members as TTF-1-driven mediators of SP-A expression and type II ce

73 Interactions between TTF-1 and GATA-6 required the amino-terminal and zinc fi

74 Likewise, mutation of the GBS blocked TTF-1 activation of the SP-A promoter.

75 nM) caused a time-dependent decrease in both TTF-1 and HNF3 in nuclear extracts and accumulation of b

76 protein-DNA interactions within the -320 bp TTF-1-responsive region of the SP-C gene, were identifie

77 nding sites was synergistically activated by TTF-1 and GATA6, and we show that TTF-1 and GATA6 physic

78 nding sites completely blocked activation by TTF-1, indicating both sites are required for TTF stimul

79 m is regulated in a combinatorial fashion by TTF-1, GATA6, and Foxa2.

80 activation of surfactant B gene promoters by TTF-1 in vitro.

81 ports the first miRNAs directly regulated by TTF-1 and clarifies how TTF-1 controls HMGA2 expression.

82 gene expression, which also is regulated by TTF-1 and Pax-8 in the thyroid, is decreased simultaneou

83 In lung cancers, TTF-1 displays seemingly paradoxical activities.

84 Transgenic mice carrying TTF-1 DNA-binding site mutations completely abolish expr

85 the EGFR-mutated NCI-H1975 and HCC827 cells, TTF-1 desensitized these cells to cisplatin; concomitant

86 In MLE cells, TTF-1-luciferase reporter constructs were activated by c

87 supershift assays were used to characterize TTF-1, HNF-3 (TGT3), and Sp1/Sp3 binding sites.

88 ed expression after co-transfection with CMV-TTF-1 in HeLa and MLE cells.

89 d SUMOylated BEND3 stabilizes NoRC component TTF-1-interacting protein 5 via association with ubiquit

90 zed these cells to cisplatin; concomitantly, TTF-1 conferred an increase in p-AKT.

91 -197 to -158 segment contained two consensus TTF-1 sites, which were specifically identified as TTF-1

92 s TTF-1 expression and controls constitutive TTF-1 levels.

93 Conversely, TTF-1 counters epithelial to mesenchymal transition in c

94 that the ability of pioglitazone to decrease TTF-1 expression contributes to its pro-adipogenic actio

95 TSH/cAMP decreases TTF-1 complex formation with the silencer, thereby decre

96 d increases markers of lung differentiation (TTF-1 and Mucin 5B).

97 f TTF-1(+) lung cancer cells (designated EDM-TTF-1(+)) displayed an anti-angiogenic activity in the e

98 stimulating factor (GM-CSF) level in the EDM-TTF-1(+) conferred the antiangiogenic activities.

99 r 1 (TTF-1) to an upstream response element (TTF-1-binding element [TBE]).

100 Expression vectors encoding TTF-1 and the catalytic subunit of protein kinase A (PKA

101 II cells decreased DNA binding of endogenous TTF-1, blocked cAMP stimulation of surfactant proteins,

102 RAR DBD greatly enhanced TTF-1 homeodomain DNA binding activity to a hSP-B enhanc

103 activity but that Erm significantly enhanced TTF-1-mediated SP-C transcription.

104 g in its hyperacetylation, further enhancing TTF-1 DNA-binding and transcriptional activity.

105 hermore, in cells that lack TTF-1, exogenous TTF-1 expression dampened the inhibitory effect of TGF-b

106 mice with the highest transgene expression, TTF-1 caused severe inflammation, pulmonary fibrosis, re

107 the essential pulmonary transcription factor TTF-1 and suppressed by Egr-1 during pulmonary developme

108 by phosphorylating the transcription factor TTF-1.

109 issue-specific thyroid transcription factor (TTF-1) DNA-binding sites.

110 ortant lung-restricted transcription factors TTF-1, GATA6, and Foxa2.

111 of lung developmental transcription factors (TTF-1, NKX2-8, and PAX9) that we recently discovered as

112 Finally, TTF-1 knockdown conferred human lung cancer cells resist

113 at acetylation is an important mechanism for TTF-1 activity.

114 65 bp) and specifically to binding sites for TTF-1 and HNF3, which act as cell-specific enhancers of

115 The thyroid transcription factor 1 gene (TTF-1 or NKX2-1) is essential to lung development; howev

116 nfluences the transcription of target genes, TTF-1, and surfactant proteins.

117 irectly regulated by TTF-1 and clarifies how TTF-1 controls HMGA2 expression.

118 each of the HNF-3 binding sites in the human TTF-1 gene inhibited the binding of MLE cell nuclear pro

119 TA-6 strongly enhanced activity of the human TTF-1 gene promoter in vitro.

120 tructs containing the 5' region of the human TTF-1 gene were transfected into immortalized mouse lung

121 ar extracts, thyroid transcription factor I (TTF-1) homeodomain, hepatic nuclear factor (HNF)-3beta,

122 We recently identified TTF-1 in rat parafollicular C cells and parathyroid cell

123 [32P]orthophosphate into immunoprecipitated TTF-1 protein also was markedly increased by cyclic AMP

124 By contrast, the levels of immunoreactive TTF-1 protein were similar in nuclear extracts of contro

125 nd steroid receptor coactivator-1 (SRC-1) in TTF-1 regulation of SP-A expression.

126 The changes in TTF-1 inversely alter CaSR gene and calcitonin gene expr

127 Furthermore, alpha(7) was not detected in TTF-1-null mice and markedly increased in TTF-1-overexpr

128 abolished the cyclic AMP-induced increase in TTF-1 DNA-binding activity.

129 in TTF-1-null mice and markedly increased in TTF-1-overexpressing mice.

130 In cultured type II cells, cAMP increased TTF-1 acetylation.

131 mbers of the HNF-3/forkhead family influence TTF-1 gene expression, deletion constructs containing th

132 miR-200 antagonists inhibited TTF-1 and surfactant proteins and up-regulated TGF-beta2

133 ed incorporation of [32P]orthophosphate into TTF-1 protein; however, the DNA binding activity of TTF-

134 Furthermore, in cells that lack TTF-1, exogenous TTF-1 expression dampened the inhibitor

135 Mechanistically, TTF-1 induced a reduction in p-AKT (S473), which in turn

136 This suggests that cAMP-mediated TTF-1 phosphorylation facilitates interaction with CBP a

137 S. pombe Reb1p is 24-29% identical to mouse TTF-1 (transcription termination factor-1) and Saccharom

138 the VEGF promoter element contains multiple TTF-1-responsive sequences.

139 ses epigenetic changes that permit access of TTF-1 and NF-kappaB to the SP-A promoter.

140 alpha. and HFH-8 inhibited the activation of TTF-1-luciferase by HNF-3beta.

141 study highlights the enigmatic activities of TTF-1 in lung cancer, and calls for future research to o

142 w miRNAs influence the oncogenic activity of TTF-1 and the role of TTF-1 in cholesterol metabolism.

143 The activity of TTF-1 is influenced by its interactions with other trans

144 rovides a mechanism by which the activity of TTF-1 on target genes is modulated.

145 , increasing the transcriptional activity of TTF-1 on the SP-C promoter.

146 rotein; however, the DNA binding activity of TTF-1 was decreased in nuclear extracts of TPA-treated t

147 Moreover, the transcriptional activity of TTF-1 was suppressed by cotransfection of a dominant neg

148 occludin, which represents another avenue of TTF-1-mediated metastasis suppression.

149 to the metastasis-critical signaling axis of TTF-1 to HMGA2, we used both reverse and forward strateg

150 ouse SP-A promoter is mediated by binding of TTF-1 at four distinct cis-active sites located in the 5

151 Binding of TTF-1 to GATA-6 required the homeodomain of TTF-1, but o

152 romoter activity, RNA levels, and binding of TTF-1 to these genes are, respectively, decreased or inc

153 dent and is mediated by increased binding of TTF-1/Nkx2.1 and NF-kappaB to a critical response elemen

154 the cohort of patients with coactivation of TTF-1 and NKX2-8 pathways appears to be resistant to sta

155 or prognosis associated with coactivation of TTF-1 and NKX2-8 was validated in 2 other independent cl

156 Coexpression of TTF-1 and PKA-cat increased fusion gene expression 3-4-f

157 RP-2 interacted via the C-terminal domain of TTF-1.

158 at TAZ binds to the NH(2)-terminal domain of TTF-1.

159 ither carboxyl- or amino-terminal domains of TTF-1.

160 Surprisingly, the EDM of TTF-1(+) lung cancer cells (designated EDM-TTF-1(+)) dis

161 the region -399 to -256 influence effects of TTF-1 on SP-A promoter activity.

162 Increased expression of TTF-1 altered alveolarization and caused chronic pulmona

163 In human lung cancer, the expression of TTF-1 and GM-CSF exhibits a statistically significant an

164 least in part by altering the expression of TTF-1 and its potential targets.

165 e expression without affecting expression of TTF-1 in doxycycline-treated double-transgenic mice.

166 sion 3-4-fold as compared with expression of TTF-1 in the absence of PKA-cat.

167 -like morphology and decreased expression of TTF-1, aquaporin-5 (AQP5), zonula occludens-1 (ZO-1), an

168 Increased expression of TTF-1, however, caused dose-dependent alterations in pos

169 s is critical for constitutive expression of TTF-1; TG decreases NFI binding to the NFI elements in a

170 GA2) mediates the antimetastatic function of TTF-1.

171 d the combined N terminus and homeodomain of TTF-1 were critical for this interaction.

172 TTF-1 to GATA-6 required the homeodomain of TTF-1, but optimal interactions with GATA-6 required the

173 We suggest that a PKA-induced increase of TTF-1 phosphorylation and TBE binding activity mediates

174 gene expression in a process independent of TTF-1.

175 ion occurred through a direct interaction of TTF-1 with the OCLN and CLDN1 promoters.

176 In contrast, higher expression levels of TTF-1 disrupted alveolar septation, causing emphysema.

177 Sites and levels of TTF-1 expression vary during lung morphogenesis and foll

178 er involves cytoplasmic trapping and loss of TTF-1 and HNF3 from the nucleus.

179 Finally, the conditioned media of TTF-1-transefected cells sensitized TTF-1(-) cells to ci

180 Modulation of TTF-1 gene expression by members of the HNF-3/forkhead f

181 A substitution mutation of TTF-1 (Thr9 two head right arrow Ala) abolished phosphor

182 Mutation of TTF-1-binding sites (TBE) 1, 3, and 4 in combination mar

183 Modest overexpression of TTF-1 caused type II cell hyperplasia and increased the

184 hibited the PKA-dependent phosphorylation of TTF-1 in vitro.

185 Phosphorylation of TTF-1 mediates PKA-dependent activation of surfactant pr

186 In the presence of TTF-1, TAZ synergistically activated the expression of m

187 was associated with increased recruitment of TTF-1, NF-kappaB, PCAF, and CBP, as well as enhanced ace

188 cAMP and IL-1 stimulated the recruitment of TTF-1, p65, CBP, and steroid receptor coactivator 1 to t

189 within 1.15 kb of the 5' flanking region of TTF-1.

190 on, demonstrating that precise regulation of TTF-1 is critical for homeostasis in the postnatal lung.

191 be under a direct and positive regulation of TTF-1.

192 -33a) is under direct positive regulation of TTF-1.

193 TG mediates the repression of TTF-1 gene expression by decreasing NFI RNA and protein

194 oncogenic activity of TTF-1 and the role of TTF-1 in cholesterol metabolism.

195 In order to determine the role of TTF-1 in lung formation, transgenic mice were generated

196 h has been conducted to investigate roles of TTF-1 in lung cancer biology.

197 ocated 96-101 base pairs from major start of TTF-1 gene transcription.

198 phosphorylation site at the NH2 terminus of TTF-1.

199 AZ mRNA and protein overlapped with those of TTF-1 and surfactant protein C (SP-C) in the respiratory

200 Through both gain- and loss-of-TTF-1 expression strategies, we show that TTF-1 positive

201 of the mouse WNT7b promoter containing only TTF-1 binding sites was synergistically activated by TTF

202 cell line with negligible endogenous NFI or TTF-1 activity.

203 nced hSP-A promoter activation by SRC-1 plus TTF-1.

204 ining 1.1 kb of the mouse alpha(7) promoter, TTF-1, and Egr-1.

205 , HNF-3 beta, HFH-4; the homeodomain protein TTF-1; and N-myc, are all expressed in the developing pu

206 Surfactant proteins, TTF-1, and aquaporin 5 expression were conditionally ind

207 with MLE cell nuclear extracts and purified TTF-1 homeodomain protein.

208 ncation/deletion studies showed that the RAR-TTF-1 interaction was mediated through the RAR DNA bindi

209 rt a model in which RAR/retinoid X receptor, TTF-1, and coactivators (p160 members and CBP) form an e

210 rt a model in which RAR/retinoid X receptor, TTF-1, coactivators, and CBP form a transcription activa

211 Recombinant TTF-1 was phosphorylated by purified PKA catalytic subun

212 Adenoviral E1A overexpression reduced TTF-1 +/- SRC-1 induction of SP-A promoter activity, sug

213 The unifying theory regarding TTF-1 is that it exhibits both pro-oncogenic and anti-me

214 order to test whether GATA factors regulated TTF-1 gene transcription, GATA-5 and -6 expression vecto

215 ts, at bp -264 to -153, positively regulates TTF-1 expression and controls constitutive TTF-1 levels.

216 Ligands or signals regulating TTF-1 levels in lung or neural tissue are unknown.

217 Surprisingly, pioglitazone repressed TTF-1 expression in PPFP-expressing thyrocytes.

218 media of TTF-1-transefected cells sensitized TTF-1(-) cells to cisplatin, implicating that the TTF-1-

219 lung-specific transcription pathways (SFTPB, TTF-1), cell adhesion, and signal transduction.

220 tathionine transferase pull-down assay shows TTF-1 direct interaction with the SRC-1 histone acetyltr

221 g adenocarcinomas, we observed that silenced TTF-1 expression down-regulated occludin, which we suppo

222 gists to identify lung adenocarcinomas since TTF-1 is expressed in 60 ~ 70% of lung ADs.

223 ) transcription in vitro by binding specific TTF-1 regulatory elements in the mouse alpha(7) promoter

224 Here, we demonstrate that TTF-1 transactivated the expression of the epithelial ti

225 g cotransfection assays, we demonstrate that TTF-1, GATA6, and Foxa2 can trans-activate the WNT7b pro

226 atin immunoprecipitation, we determined that TTF-1 binds to the promoter of SREBF2, the host gene of

227 n summary, this study provides evidence that TTF-1 may reprogram lung cancer secreted proteome into a

228 We found that TTF-1 DNA binding activity was increased in nuclear extr

229 We hypothesize that TTF-1 similarly coordinates Ca2+-dependent gene expressi

230 Our data indicate that TTF-1 interacts with PPFP to inhibit the pro-adipogenic

231 tivated by TTF-1 and GATA6, and we show that TTF-1 and GATA6 physically interact in vivo.

232 In this report, we show that TTF-1 is present in the parafollicular C cells of multip

233 of-TTF-1 expression strategies, we show that TTF-1 positively regulates vascular endothelial growth f

234 kdown and overexpression studies showed that TTF-1 inhibits PPFP target gene expression and impairs a

235 rophoretic mobility shift assays showed that TTF-1, GATA6, and Foxa2 can bind to a specific subset of

236 Our study shows that TTF-1 sensitizes the KRAS-mutated A549 and NCI-H460 lung

237 Collectively, these data suggest that TTF-1 transcriptionally regulates occludin, which repres

238 of HMGA2 without the 3'-UTR, suggesting that TTF-1 keeps the prometastasis gene HMGA2 in check via up

239 ulating cholesterol metabolism suggests that TTF-1 may be a modulator of cholesterol homeostasis in t

240 We show, therefore, that TTF-1 is a Ca2+-modulated transcription factor that coor

241 The TTF-1-dependent upregulation of VEGF was moderately sens

242 The TTF-1-induced VEGF upregulation occurs in both compartme

243 ugh the RAR DNA binding domain (DBD) and the TTF-1 homeodomain.

244 at the lysine residue at position 182 in the TTF-1 HD is acetylated in respiratory epithelial cells.

245 ected mutagenesis of the GATA element in the TTF-1 promoter region inhibited transactivation by GATA-

246 Coactivation of the TTF-1 and NKX2-8 pathways identified a cluster of patien

247 ancer cell lines showing coactivation of the TTF-1 and NKX2-8 pathways were shown to exhibit resistan

248 Site-specific mutagenesis of either of the TTF-1 binding sites completely blocked activation by TTF

249 identified elements in the 5' region of the TTF-1 gene that bound MLE cell nuclear proteins consiste

250 ther increase the secreted VEGF level of the TTF-1(+) lung cancer cells.

251 anscription by suppressing expression of the TTF-1, TTF-2, and Pax-8 genes.

252 However, mutation of the TTF-1-binding sites alone or in combination decreased GA

253 roteins and inhibited transactivation of the TTF-1-luciferase constructs after cotransfection with HN

254 t the region between bp -264 and -153 on the TTF-1 promoter contains two nuclear factor I (NFI) eleme

255 (-) cells to cisplatin, implicating that the TTF-1-driven chemosensitization activity may be dually p

256 cell nuclear extracts reduced binding to the TTF-1 binding element upstream of SP-A gene.

257 translation and directly interacted with the TTF-1-binding element in the sp-C promoter.

258 f cytokeratin 18, E-cadherin, thyroglobulin, TTF-1 and Pax-8 proteins.

259 TAZ binds to TTF-1, increasing the transcriptional activity of TTF-1

260 endent gene expression in all cells in which TTF-1 and the CaSR are expressed, i.e., parathyroid cell

261 ion, transgenic mice were generated in which TTF-1 was expressed in respiratory epithelial cells of w

262 ation of favorable prognosis associated with TTF-1(+) lung adenocarcinomas.

263 GATA-6 is co-expressed with TTF-1 in the respiratory epithelium in vivo and respirat

264 ells (MLE-15 cells), being co-expressed with TTF-1 mRNA.

265 -C gene transcription when co-expressed with TTF-1, supporting the concept that GATA-6 and TTF-1 migh

266 e lung epithelial cell (MLE15) extracts with TTF-1 and was identified by mass spectrometry.

267 munoprecipitated from the cell extracts with TTF-1.

268 PARP-2 and PARP-1 interact with TTF-1 and regulate the expression of surfactant protein

269 e hypothesis that NFI isoforms interact with TTF-1 to differentially regulate SP-C transcription, we

270 thesis that NFI family members interact with TTF-1 to regulate type II cell function.

271 Although GATA-4 also interacted with TTF-1 in two-hybrid assays, GATA-4 was not as active as

272 trated that the TAZ directly interacted with TTF-1.

273 tion, which results from an interaction with TTF-1.

274 Cotransfection of NFI family members with TTF-1 induced synergistic activation of the SP-C promote

275 timally manage chemotherapy of patients with TTF-1(+) lung ADs.

276 GST-GATA-6 directly co-precipitated with TTF-1 after in vitro translation and directly interacted

277 rgistic activation of the sp-C promoter with TTF-1.

278 in the activation of the sp-C promoter with TTF-1.

279 ns, CBP and SRC-1 acted synergistically with TTF-1 to increase SP-A promoter activity.

280 ter, binding and acting synergistically with TTF-1.

281 expression vectors were co-transfected with TTF-1 luciferase expression vector.