ライフサイエンスコーパス: Thermotoga

1 cina, Pseudomonas, Bartonella, Nitrosomonas, Thermotoga, and Aquifex showed a strong preference for L

2 a role in several lineages, such as Aquifex, Thermotoga, and Fusobacterium.

3 oplasmatales and M. thermoautotrophicus with Thermotoga, and Halobacteriales with Actinobacteria, sug

4 Studies of the Escherichia, Neisseria, Thermotoga, and Mycobacteria clustered regularly intersp

5 proteins identifies Aquifex as grouping with Thermotoga another bacterial hyperthemophile belonging t

6 of G4 structural stability and integrity in Thermotoga compared to Pseudothermotoga.

7 uctural differences were observed instead in Thermotoga compared to Thermoplasmatales and M. thermoau

8 es of the bacterial phylogenetic tree, i.e., Thermotoga, Deinococcus-Thermus, Cyanobacteria, spiroche

9 g DNA, a property that distinguishes it from Thermotoga endonuclease V.

10 The copper transport ATPase from Thermotoga maritima (CopA) provides a useful system for

11 Crystals of DAHPS from Thermotoga maritima (DAHPS(Tm)) were grown in the presen

12 of peptide chain release factor 1 (RF1) from Thermotoga maritima (gi 4981173) at 2.65 Angstrom resolu

13 In this study, we characterized LarE from Thermotoga maritima (LarE(Tm)) and show that it uses the

14 tructure of a type III PanK, the enzyme from Thermotoga maritima (PanK(Tm)), solved at 2.0-A resoluti

15 tructures of ODP from Td and the thermophile Thermotoga maritima (Tm) in the Fe[III](2)-O(2) (2-), Zn

16 crystal structures of complexes between the Thermotoga maritima (Tm) NadA K219R/Y107F variant and (i

17 tm0322) from the hyperthermophilic bacterium Thermotoga maritima (TM0322).

18 Mechanistic studies of a FDTS from Thermotoga maritima (TM0449) are presented here.

19 ed three-dimensional structure of GK-II from Thermotoga maritima (TM1585; PDB code 2b8n) revealed a n

20 Thermotoga maritima (Tma) EndoV recognizes and primarily

21 rofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) are presented.

22 rofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) has been examined by enzyme

23 R) and the dimeric, thermophilic enzyme from Thermotoga maritima (TmDHFR).

24 al analysis of GH36 alpha-galactosidase from Thermotoga maritima (TmGalA).

25 altodextrin periplasmic-binding protein from Thermotoga maritima (tmMBP) complexed with oligosacchari

26 dge gap, we have characterized the MetY from Thermotoga maritima (TmMetY).

27 N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in tran

28 of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPas

29 me bacteria, including the hyperthermophiles Thermotoga maritima and Aquifex aeolicus.

30 Co(2+)-requiring alkaline phosphatases from Thermotoga maritima and Bacillus subtilis have a His and

31 Alkaline phosphatases from organisms such as Thermotoga maritima and Bacillus subtilis require cobalt

32 ochemical properties of a TF:S7 complex from Thermotoga maritima and determined its crystal structure

33 We have created a series of Thermotoga maritima and Escherichia coli pseudouridine 5

34 We expressed and purified MpgII from Thermotoga maritima and found that the enzyme releases b

35 d the crystal structure of the PanK-III from Thermotoga maritima and identified it as a member of the

36 Co-cultivation of the hyperthermophiles Thermotoga maritima and Methanococcus jannaschii resulte

37 ze and engineer TP-shell interactions in the Thermotoga maritima and Myxococcus xanthus encapsulin sy

38 eus, and two from the Gram-negative bacteria Thermotoga maritima and Pseudomonas aeruginosa.

39 ze the in situ cell envelope architecture of Thermotoga maritima and show that the toga is made of ex

40 have characterized the ECF transporters from Thermotoga maritima and Streptococcus thermophilus.

41 of HU from the hyperthermophilic eubacterium Thermotoga maritima are shown here to differ significant

42 rate-binding domains of HemK from E.coli and Thermotoga maritima are structurally similar, despite th

43 Using the hydrogenase maturase HydE from Thermotoga maritima as a template, we obtained several u

44 ion crystal structure of lysine bound to the Thermotoga maritima asd lysine riboswitch ligand-binding

45 ucible transcriptional repressor, HrcA, from Thermotoga maritima at 2.2A resolution.

46 of the PLP synthase complex (YaaD-YaaE) from Thermotoga maritima at 2.9 A resolution.

47 structure of the FliY catalytic domain from Thermotoga maritima bears strong resemblance to the midd

48 The open reading frame TM1643 of Thermotoga maritima belongs to a large family of protein

49 In Thermotoga maritima both candidate genes (in an original

50 ed to map conformational states of CorA from Thermotoga maritima by determining which residues suppor

51 -examined the completely sequenced genome of Thermotoga maritima by employing the combined use of the

52 haracterize the CheA-receptor interaction in Thermotoga maritima by NMR spectroscopy and validate the

53 We show here that the Cmr complex from Thermotoga maritima can cleave an ssRNA target that is c

54 owever, we show that both RimO and MiaB from Thermotoga maritima catalyze methyl transfer from SAM to

55 previously isolated by random sequencing of Thermotoga maritima cDNA clones.

56 The 2.6 A resolution crystal structure of Thermotoga maritima CheA (290-671) histidine kinase reve

57 cture of a soluble ternary complex formed by Thermotoga maritima CheA (TmCheA), CheW, and receptor si

58 investigate the two ATP-binding sites of the Thermotoga maritima CheA dimer (TmCheA) and the single s

59 The structure of Thermotoga maritima CheA domain P2 in complex with CheY

60 of nucleotide binding to the active site of Thermotoga maritima CheA was investigated using stopped-

61 ity of the phospho-accepting His (His-45) in Thermotoga maritima CheA.

62 thylation analyses utilizing S. enterica and Thermotoga maritima CheR proteins and MCPs indicate that

63 sed on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architect

64 rystal structures of a fragment of MetH from Thermotoga maritima comprising the domains that bind Hcy

65 ities, while the hyperthermophilic bacterium Thermotoga maritima contains only one, pyruvate ferredox

66 Thermotoga maritima CorA (TmCorA) is the only member of

67 The crystal structures of Thermotoga maritima CorA provide an excellent structural

68 The crystal structure of Thermotoga maritima CorA shows a homopentamer with two t

69 eolicus GyrA/ParC CTD with the GyrA CTD from Thermotoga maritima creates an enzyme that negatively su

70 scattering studies of thermostable CheA from Thermotoga maritima determine that the His-containing su

71 hile those of Mycobacterium tuberculosis and Thermotoga maritima did not.

72 ffector from the hyperthermophilic bacterium Thermotoga maritima discriminates between native and inv

73 ymerase derived from Thermus species Z05 and Thermotoga maritima DNA polymerases.

74 ed the 1.8-A resolution crystal structure of Thermotoga maritima DrrB, providing a second structure o

75 e systematically designed 24 variants of the Thermotoga maritima encapsulin cage, featuring pores of

76 In the case of the Thermotoga maritima encapsulin, the decameric cargo prot

77 The gene buk2 from Thermotoga maritima encodes a member of the ASKHA (aceta

78 nserved residues D43, E89, D110, and H214 in Thermotoga maritima endonuclease V catalysis.

79 was performed in nine conserved positions of Thermotoga maritima endonuclease V to identify amino aci

80 t seven conserved motifs of the thermostable Thermotoga maritima endonuclease V to probe for residues

81 Thermotoga maritima exo-beta-fructosidase (BfrA) secrete

82 , we showed that FMN-free diferrous FDP from Thermotoga maritima exposed to 1 equiv NO forms a stable

83 ors with a single enzyme 'model system', the Thermotoga maritima family 1 beta-glucosidase, TmGH1.

84 he hyperthermophilic and anaerobic bacterium Thermotoga maritima ferments a wide variety of carbohydr

85 interaction between the N-terminal domain of Thermotoga maritima FliG (FliG(N)) and peptides correspo

86 Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex str

87 e (6PGDH) from a hyperthermophilic bacterium Thermotoga maritima from its natural coenzyme NADP(+) to

88 The crystal structures of Thermotoga maritima GTase and its complex with the inhib

89 lease V from the hyperthermophilic bacterium Thermotoga maritima has been cloned and expressed in Esc

90 Thermotoga maritima HpkA is a transmembrane histidine ki

91 at HU from the hyperthermophilic eubacterium Thermotoga maritima HU bends DNA and constrains negative

92 presents a 2.3-kb locus with similarity to a Thermotoga maritima hypothetical protein, while another

93 ty, we expressed the gyrase of the bacterium Thermotoga maritima in a naive archaeon Thermococcus kod

94 report three crystal structures of ThiI from Thermotoga maritima in complex with a truncated tRNA.

95 ion of a sensor HK, one from the thermophile Thermotoga maritima in complex with ADPbetaN at 1.9 A re

96 heterologously produced the NfnAB complex of Thermotoga maritima in Escherichia coli, provided kineti

97 ve solved the crystal structure of FtsA from Thermotoga maritima in the apo and ATP-bound form.

98 ed five different structures of FGAR-AT from Thermotoga maritima in the presence of substrates, a sub

99 production of the GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants and demonst

100 Thermotoga maritima is a marine hyperthermophilic microo

101 work, a structure of the PurLQS complex from Thermotoga maritima is described revealing a 2:1:1 stoic

102 Structural characterization of Thermotoga maritima IscU by CD and high resolution NMR y

103 of the 174-nucleotide sensing domain of the Thermotoga maritima lysine riboswitch in the lysine-boun

104 Here we report that Thermotoga maritima MazG protein (Tm-MazG), the product

105 pe and C150/154/157A triple variant forms of Thermotoga maritima MiaB have revealed the presence of t

106 ts of seven distinct bisphosphonates against Thermotoga maritima mPPase to explore their mode of acti

107 dy the assembly and mechanical properties of Thermotoga maritima MreB in the presence of different nu

108 Here, we studied the assembly of Thermotoga maritima MreB triggered by ATP in vitro and c

109 A whole-genome alignment of Thermotoga maritima MSB8 and Thermotoga neapolitana NS-E

110 The 1,860,725-base-pair genome of Thermotoga maritima MSB8 contains 1,877 predicted coding

111 sequence of the hyperthermophilic bacterium Thermotoga maritima MSB8 presents evidence for lateral g

112 The hyperthermophilic bacterium Thermotoga maritima MSB8 was grown on a variety of carbo

113 , we explore these questions using the model Thermotoga maritima nanocompartment known to encapsulate

114 Molecular replacement with the Thermotoga maritima NifS model was used to determine pha

115 ng growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysacchar

116 ined, the crystal structure was reported for Thermotoga maritima PlsC, an enzyme in the same gene fam

117 The extremophile Thermotoga maritima possesses a remarkable array of carb

118 The hyperthermophilic eubacterium Thermotoga maritima possesses an operon encoding an Hsp7

119 Endonuclease V obtained from Thermotoga maritima preferentially cleaves purine mismat

120 n success, DXMS analysis was attempted on 24 Thermotoga maritima proteins with varying crystallizatio

121 C-terminal amphipathic helix in vitro using Thermotoga maritima proteins.

122 Here, we report the analysis of the Thermotoga maritima proteome, in which we compare the pr

123 The crystal structures of Thermotoga maritima Psi55S, and its complex with RNA, ha

124 cture of this C-terminal domain of FliN from Thermotoga maritima revealed a saddle-shaped dimer forme

125 ure of the FliM middle domain (FliM(M)) from Thermotoga maritima reveals a pseudo-2-fold symmetric to

126 main (P1) from the chemotaxis kinase CheA of Thermotoga maritima reveals a remarkable degree of struc

127 at 3.85 A resolution of the RNA component of Thermotoga maritima ribonuclease P.

128 es (F17 and R89) are essential for efficient Thermotoga maritima RNase P activity.

129 We report the crystal structure of the Thermotoga maritima RNase P holoenzyme in complex with t

130 at the cytoplasmic helix-turn-helix motif of Thermotoga maritima RodZ directly interacts with monomer

131 The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant struc

132 Here, we report a crystal structure of Thermotoga maritima SecA at 1.9 A resolution.

133 The recent crystal structure of the Thermotoga maritima SecA-SecYEG complex shows the ATPase

134 ur data support the in vivo relevance of the Thermotoga maritima SecA.SecYEG crystal structure that v

135 iety of contexts, including the structure of Thermotoga maritima sigmaA region 4 described herein.

136 itor binding to potently inhibited Sirt1 and Thermotoga maritima Sir2 and to moderately inhibited Sir

137 of the N and C-terminal globular domains of Thermotoga maritima SMC in Escherichia coli by replacing

138 The 2.17 A resolution crystal structure of a Thermotoga maritima soluble receptor (Tm14) reveals dist

139 occasionally found also in bacteria such as Thermotoga maritima that do not utilise a PEP-PTS system

140 f loop 2, which are analogous to residues in Thermotoga maritima that mediate cross-talk.

141 we report the structures of hTK1 and of the Thermotoga maritima thymidine kinase (TmTK) in complex w

142 The Thermotoga maritima TM0065 gene codes for a protein (TM-

143 o be a potent inhibitor (Ki = 8.2 nM) of the Thermotoga maritima TmGH1 beta-glucosidase.

144 olution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate a suite of catalysts for

145 The gene for the Thermotoga maritima Trk potassium transporter component

146 TruB apo enzyme, as well as the structure of Thermotoga maritima TruB in complex with RNA.

147 tform called OrthoRep, we rapidly evolve the Thermotoga maritima tryptophan synthase beta-subunit (Tm

148 287/288 from the hyperthermophilic bacterium Thermotoga maritima using all-atom molecular dynamics (M

149 RNase III of the hyperthermophilic bacterium Thermotoga maritima was analyzed using purified recombin

150 synthase from the thermophilic microorganism Thermotoga maritima was cloned, and the enzyme was overe

151 protein from the hyperthermophilic bacterium Thermotoga maritima was determined at 1.2-A resolution b

152 Ribosome recycling factor (RRF) of Thermotoga maritima was expressed in Escherichia coli fr

153 Previously, Tm0936 from Thermotoga maritima was shown to catalyze the deaminatio

154 NagA from Thermotoga maritima was shown to require a single divale

155 mophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expresse

156 ated interactions between FliM and FliG from Thermotoga maritima with X-ray crystallography and pulse

157 Thermotoga maritima XseA/B homologs TM1768 and TM1769 we

158 as inhibitors of a polytopic PGT (WecA from Thermotoga maritima) and a monotopic PGT (PglC from Camp

159 Bacillus subtilis, Sulfolobus tokodaii, and Thermotoga maritima) and two eukaryotic (Saccharomyces c

160 chaeoglobus fulgidus, and from the bacterium Thermotoga maritima) into the E. coli expression vector

161 les but later recolonized a hot environment (Thermotoga maritima) relied in their evolutionary strate

162 M0487 (a 102-residue alpha+beta protein from Thermotoga maritima), we predicted the complete, topolog

163 in vitro methylation of chemoreceptors from Thermotoga maritima, a hyperthermophile that has served

164 prokaryotes and eukaryotes, was cloned from Thermotoga maritima, a hyperthermophilic bacterium.

165 o acid sequence level to the enzyme found in Thermotoga maritima, a thermophilic eubacteria, and sugg

166 cture of an M42 aminopeptidase, TmPep1050 of Thermotoga maritima, along with the dodecamer structure.

167 a-glycosidases from Sulfolobus solfataricus, Thermotoga maritima, and Caldocellum saccharolyticum.

168 P superfamilies against the entire genome of Thermotoga maritima, and make over a 100 new fold predic

169 ic organism, the hyperthermophilic bacterium Thermotoga maritima, and those of close homologs from me

170 r to the KH1 domain of the NusA protein from Thermotoga maritima, another cold-shock associated RNA-b

171 9 family proteins from a variety of sources (Thermotoga maritima, Bacillus subtilis, Acinetobacter ba

172 f an enzyme of unknown activity, Tm0936 from Thermotoga maritima, by docking high-energy intermediate

173 glucose-6-phosphate dehydrogenase (Gpd) from Thermotoga maritima, demonstrated robust activity over a

174 arrel domain from the thermophilic bacteria, Thermotoga maritima, enabled an NMR-based site-specific

175 te of CspTm, a small cold-shock protein from Thermotoga maritima, engineered to contain a single tryp

176 -isopentenyladenosine of tRNA in E. coli and Thermotoga maritima, has been demonstrated to harbor two

177 HK853 and its response regulator RR468 from Thermotoga maritima, here we report a pH-mediated confor

178 hemical studies demonstrate that TM1635 from Thermotoga maritima, originally annotated as a putative

179 Surprisingly, even Thermotoga maritima, previously considered to have only

180 ith Lactobacillus casei, and 23 and 40% with Thermotoga maritima, respectively.

181 Unlike endonuclease V from Thermotoga maritima, Salmonella endonucleae V can only t

182 sophile Escherichia coli and the thermophile Thermotoga maritima, subunit dissociation activates at t

183 nate lyase from the thermophilic eubacterium Thermotoga maritima, the archaebacterial lyase contains

184 he genome of the hyperthermophilic bacterium Thermotoga maritima, TM0504 encodes a putative signaling

185 otein from the hyperthermostable eubacterium Thermotoga maritima, TmHU as an efficient gene transfer

186 ding by the EcfS subunit for riboflavin from Thermotoga maritima, TmRibU.

187 In Thermotoga maritima, two PhoU homologues have been ident

188 conserved in simulations of the mPPases from Thermotoga maritima, Vigna radiata and Clostridium leptu

189 domains from a hyperthermophilic bacterium, Thermotoga maritima, was cloned and expressed in Escheri

190 it of RNase P from a thermophilic bacterium, Thermotoga maritima, was overexpressed in and purified f

191 e conserved hypothetical protein TM0979 from Thermotoga maritima, we demonstrate the capabilities of

192 rototyping it using the simple microorganism Thermotoga maritima, we show our model accurately simula

193 atabolite-linked transcriptional networks in Thermotoga maritima, we used full-genome DNA microarray

194 he heterodimeric ABC exporter TM287/288 from Thermotoga maritima, which contains a non-canonical ATP

195 f a larger fragment of the FliG protein from Thermotoga maritima, which encompasses the middle and C-

196 omes of several unusual organisms, including Thermotoga maritima, whose genome reveals extensive pote

197 ion of P1 and P3P4 from the hyperthermophile Thermotoga maritima.

198 UvrB from Bacillus caldotenax and UvrC from Thermotoga maritima.

199 tal structure of most of the FliN protein of Thermotoga maritima.

200 sequence of the hyperthermophilic bacterium Thermotoga maritima.

201 structure of the C-terminal 70% of FliG from Thermotoga maritima.

202 d loop of the group I NifS-like protein from Thermotoga maritima.

203 opic labeling of a sigma70-like subunit from Thermotoga maritima.

204 o the proteome of the thermophilic bacterium Thermotoga maritima.

205 genome of Mycoplasma genitalium, and 23% in Thermotoga maritima.

206 the crystal structure to 2.2 A of MinC from Thermotoga maritima.

207 n was detected in the thermophilic bacterium Thermotoga maritima.

208 minators of this type, with the exception of Thermotoga maritima.

209 he genome of the hyperthermophilic bacterium Thermotoga maritima.

210 liG-C from the hyperthermophilic eubacterium Thermotoga maritima.

211 Here, we describe a UDG from the thermophile Thermotoga maritima.

212 sequences in the hyperthermophilic bacterium Thermotoga maritima.

213 ay in the marine hyperthermophilic bacterium Thermotoga maritima.

214 gulator from the hyperthermophilic bacterium Thermotoga maritima.

215 ecombinant proteins from the model bacterium Thermotoga maritima.

216 he carbohydrate and nucleotide metabolism of Thermotoga maritima.

217 in ThyX from the hyperthermophilic bacterium Thermotoga maritima.

218 protein in the hydrogen-producing bacterium Thermotoga maritima.

219 raction modes of chemoreceptor and CheW from Thermotoga maritima.

220 e sugar kinome in the thermophilic bacterium Thermotoga maritima.

221 otoga sp. strain RQ2 is probably a strain of Thermotoga maritima.

222 eW and the P4-P5 fragment of CheA, both from Thermotoga maritima.

223 exonuclease activity in endonuclease V from Thermotoga maritima.

224 amined a deflavinated FDP (deflavo-FDP) from Thermotoga maritima.

225 catalysis by DHFR from the hyperthermophile Thermotoga maritima.

226 e central metabolic network of the bacterium Thermotoga maritima.

227 5 angstrom (A), obtained for components from Thermotoga maritima.

228 we have characterized an ExoVII homolog from Thermotoga maritima.

229 ichia coli, and purified untagged MreB1 from Thermotoga maritima.

230 This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Therm

231 Thermotoga neapolitana (Tne) DNA polymerase belongs to t

232 epresentative from the thermophilic organism Thermotoga neapolitana (TnIYD).

233 Thermotoga neapolitana 1,4-beta-d-glucan glucohydrolase

234 Construction of a Thermotoga neapolitana adenylate kinase (AK) library usi

235 acillus subtilis adenylate kinase (BsAK) and Thermotoga neapolitana adenylate kinase (TnAK) with iden

236 Backbone conformational dynamics of Thermotoga neapolitana adenylate kinase in the free form

237 me alignment of Thermotoga maritima MSB8 and Thermotoga neapolitana NS-E has revealed numerous large-

238 Characterization in Thermotoga neapolitana of a catabolic gene cluster encod

239 1,4-beta-D-glucan glucohydrolase (GghA) from Thermotoga neapolitana.

240 thermostable two-domain endo-acting ABN from Thermotoga petrophila (TpABN) revealed how some GH43 ABN

241 report on the ligand-bound structure of the Thermotoga petrophila fluoride riboswitch, which adopts

242 The fluoride sensing riboswitch from Thermotoga petrophila is a complex regulatory RNA propos

243 the thermostable beta-glucosidase (TPG) from Thermotoga petrophlia showed potential to enhance tea ar

244 st to the SlpA/OmpM superfamily of proteins, Thermotoga possess a highly diverse bipartite OM-tetheri

245 Thermotoga sp. RQ2 differs from T. maritima in its genes

246 Thermotoga sp. strain RQ2 is probably a strain of Thermo

247 extent and consequences of gene flow within Thermotoga species and strains.

248 rees C <= Topt < 80 degrees C) species, with Thermotoga species exhibiting higher G4 stability, indic

249 The hyperthermophilic bacterium Thermotoga species strain RQ7 harbors an 846-bp plasmid,

250 e primary enzyme for attacking cellobiose in Thermotoga spp.

251 Hyperthermophilic Thermotoga spp. are excellent candidates for the biosynt

252 The Fe-AAdh gene is highly conserved in Thermotoga spp., Pyrococcus furiosus and Thermococcus ko

253 dization study was initiated to compare nine Thermotoga strains to the sequenced T. maritima MSB8.

254 .L1917 was acquired from other bacteria like Thermotoga subterranea and Cbu.L1951 from lower eukaryot

255 P motif; V substitutes for R only in HU from Thermotoga, Thermus and Deinococcus.

256 tinctive differences in G4 stability between Thermotoga (Topt >= 80 degrees C) and Pseudothermotoga (

257 phosphate suggests that the novel pathway in Thermotoga utilizes a phosphorylated derivative of inosi