ライフサイエンスコーパス: Tricine

1 as independent of the concentration of added tricine(+/-).

2 ium chloride (TEA(+)Cl(-)) or the zwitterion tricine(+/-).

3 zinc that is 200-fold higher than those for tricine and CaEDTA.

4 ned with monovalent HYNIC conjugate 15 using tricine and isonicotinic acid as coligands with HYNIC fo

5 emical yield) with Tc-99m in the presence of tricine and SnCl(2) with high specific activity (>100 MB

6 ynthesized and radiolabeled with 99mTc using tricine and trisodium triphenylphosphine-3,3',3"-trisulf

7 yl-6-hydrazinonicotinate hydrochloride using tricine as the transchelator.

8 We demonstrate that tricine, considered a preferred chelator for studying th

9 Western blot assay (muWestern) using a Tris tricine discontinuous buffer system suitable for analyse

10 Application of up to 3 mM tricine does not affect the murine b-wave in both genoty

11 at comigrates on reverse phase HPLC and Tris-tricine electrophoresis.

12 sured in low |Deltap K a| buffers containing tricine, EPPS and citrate, as well as otherwise identica

13 Tricine gel electrophoresis, followed by silver staining

14 on of peptide hydrolysis was accomplished by Tricine gel electrophoresis.

15 mass spectrometry (MALDI-TOF-TOF), and (iii) Tricine gel electrophoresis.

16 ation of tear lipocalin was confirmed by SDS tricine gradient PAGE.

17 common buffers (e.g., CHES/LiOH, TAPS/LiOH, Tricine/LiOH, MOPS/LiOH, MES/LiOH, and acetic acid/LiOH)

18 ntibody probe plug delivery scheme, the Tris tricine muWestern blot enables 40% higher separation res

19 The Tris tricine muWestern is completed in an enclosed, straight

20 thyl]methyl-2-aminoethanesulfonic acid), and tricine (N-tris[hydroxymethyl]methylglycine) also form c

21 nolamine/Capso, pH 9.6; (ii) triethanolamine/Tricine, pH 7.9; and (iii) Bis-Tris/Aces, pH 6.7.

22 mTc using a coordination chemistry involving tricine-phosphine coligands.

23 Tricine polyacrylamide gel electrophoresis analysis of o

24 and S at neutral pH by high resolution Tris-Tricine SDS-PAGE and matrix-assisted laser desorption io

25 However, Tricine SDS-PAGE in combination with MALDI-TOF-MS and ho

26 Tricine SDS-PAGE revealed stepwise truncations of the O

27 and beta-lg by staining acrylamide gel after tricine SDS-PAGE using cationic 'stains all' dye.

28 MP and B-lg by staining acrylamide gel after tricine SDS-PAGE using cationic 'stains all' dye.

29 sing the high resolving capacity of the Tris-Tricine-SDS buffer system of Schaegger and von Jagow we

30 omassie-stained, two-dimensional Blue Native/Tricine-SDS electrophoretic gels.

31 pparent molecular weight of LPS as judged by Tricine-SDS-PAGE and did not affect ability to react wit

32 rected photoaffinity analogs, proteases, and tricine-SDS-polyacrylamide gel electrophoresis.

33 ed peptides by separation on high-resolution tricine-SDS-polyacrylamide gels.

34 olypeptides determined by second-dimensional Tricine-SDS/PAGE including the 51 kDa, PSST and TYKY sub

35 g two-dimensional PAGE (blue native PAGE and tricine/SDS/PAGE) and subsequent western blots were deve

36 Tricine sodium dodecyl sulfate-polyacrylamide gel electr

37 almond proteins was quantified by performing tricine-sodium dodecyl sulfate-polyacrylamide gel electr

38 amide (HYNIC) was radiolabeled using (99m)Tc-tricine to a specific activity of 3.4-7.4 MBq/microg.

39 ne-3,3',3''-trisulfonate) and 8 ([(99m)Tc(6)(tricine)(TPPTS)]) were prepared in high yield and high s

40 (99)(m)Tc complexes 7 ([(99m)Tc(5)(tricine)(TPPTS)]: TPPTS = trisodium triphenylphosphine-3

41 Upon nonreducing Tris-Tricine-urea-SDS-PAGE, newly synthesized proinsulin from

42 s-linking reagents and were resolved using a Tricine/urea low-molecular-weight resolution gel system.

43 of this paper series; e.g., CHES, MES, MOPS, Tricine were used to demonstrate behavior of such comple